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Wang Yanyan - - 2010
Many processes in living cells have electrochemical characteristics that are suitable for measurement by potentiometric biosensors. Potentiometric biosensors allow non-invasive, real time monitoring of the extracellular environment changes by measuring the potential at cell/sensor interface. This can be used as an indicator for overall cell cytotoxicity. The present work employs ...
Martín-Navarro Carmen M - - 2010
Three different cell lines (murine macrophages, HeLa and osteosarcoma cells) were assayed in order to check for the manifestation of the cytopathic effects of three strains of Acanthamoeba recently isolated in our laboratory from contact lens cases: CLC-16, CLC-41.r and CLC-51-l. Adhesion and cytotoxicity assays were carried out with these ...
Priestley Catherine C - - 2010
Potassium bromate (KBrO3) is a well-established rodent kidney carcinogen and its oxidising activity is considered to be a significant factor in its mechanism of action. Although it has also been shown to be clearly genotoxic in a range of in vivo and in vitro test systems, surprisingly, it is not ...
Geys Jorina - - 2010
BACKGROUND: Reliable in vitro studies that generate consistent toxicity data on nanomaterials on a high-throughput scale will be of invaluable significance in the next few years. AIM: In this study, we checked the influence of several steps of the experimental design on the outcome: we investigated the role of cell ...
Chicca Andrea - - 2010
In the present study, a further investigation of the cytotoxic activity of an acetylenic constituent of Echinacea pallida roots, namely, pentadeca-(8 Z,13 Z)-dien-11-yn-2-one, was performed, revealing a concentration-dependent cytotoxicity on several human cancer cell lines, including leukemia (Jurkat and HL-60), breast carcinoma (MCF-7), and melanoma (MeWo) cells. As part of ...
Szurko Agnieszka - - 2009
Photosensitizers with desirable combinations of chemical, photophysical and biological properties are essential for improving the efficacy of photodynamic therapy (PDT) against various cancers. Chlorins seem to be promising candidates for photodynamic therapy (PDT) owing to their photophysical properties. This paper reports spectroscopic and biological properties of a novel synthetic chlorin ...
Hui Ivan Chun-Fai IC Liver Cancer and Hepatitis Research Laboratory, Department of Pathology, LKS Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong, - - 2010
The mammalian target of rapamycin (mTOR), which phosphorylates p70S6K and 4EBP1 and activates the protein translation process, is upregulated in cancers and its activation may be involved in cancer development. In this study, we investigated the tumour-suppressive effects of rapamycin and its new analogue CCI-779 on hepatocellular carcinoma (HCC). Rapamycin ...
Huang Dorothy Yu - - 2009
Microcystins are bioactive metabolites produced by cyanobacteria in water. These cyclic heptapeptides have caused public health concern worldwide. By interfering with cellular phosphorylation and signaling, microcystins can cause acute and chronic liver diseases. Therefore, the World Health Organization (WHO) has set the provisional drinking water guideline value at 1.0 microg/L ...
Chen Lixia - - 2010
Curcumin, a polyphenolic compound, is the active component of Curcuma longa and has been extensively investigated as an anticancer drug that modulates multiple pathways. Eukaryotic initiation factors (eIFs) have been known to play important roles in translation initiation, which controls cell growth and proliferation. Little is known about the effects ...
Zakharova Ol'ga A - - 2010
Four new n-butylamino and two sulfur-containing derivatives of polyfluoro-1,4-naphthoquinone were synthesized and their mutagenic and antioxidant properties in Salmonella cells, as well as the cytotoxicity in human myeloma (RPMI 8226), human mammary adenocarcinoma (MCF-7), mouse fibroblasts (LMTK) and primary mouse fibroblast cells (PMF) were studied. 2-n-Butylamino-3,5,6,7,8-pentafluoro-1,4-naphthoquinone (1) showed efficient inhibition ...
Hao Gangping - - 2010
Treatment of suspension cells of Ginkgo biloba with fungal endophytes resulted in accumulation of flavonoids, increased abscisic acid (ABA) production and activation of phenylalanine ammonia-lyase (PAL). Fluridone, an inhibitor of ABA biosynthesis, was effective in inhibiting fungal endophytes-induced ABA biosynthesis, increase of PAL activity and flavonoids accumulation. Moreover, exogenous application ...
Costea Daniela Elena - - 2010
BACKGROUND: The high incidence of oral cancer in Sudan has been associated with the use of toombak, the local type of smokeless tobacco. However, its specific effects on human oral cells are not known. We aimed to investigate the effects of toombak on primary normal human oral keratinocytes, fibroblasts, and ...
Umeyama Akemi - - 2010
A screening of 30 crude extracts of marine sponges against human promyelocytic leukemia cells (HL-60) yielded an EtOAc extract of the sponge Callyspongia sp. (Callyspongiidae) with significant activity. Further bioassay-guided fractionation of the EtOAc extract led to the isolation of three polyacetylene metabolites: a new polyacetylene diol, callyspongidiol (1), along ...
Wielgus Albert R - - 2010
The water-soluble nanoparticle hydroxylated fullerene [fullerol, nano-C60(OH)(22-26)] has several clinical applications including use as a drug carrier to bypass the blood ocular barriers. We have previously found that fullerol is both cytotoxic and phototoxic to human lens epithelial cells (HLE B-3) and that the endogenous antioxidant lutein blocked some of ...
Zhou Yaping - - 2010
We use quartz crystal microbalance (QCM), 3-(4,5-dimethylthizaol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and fluorescence/optical microscopic inspections to investigate the cytotoxicity of paramagnetic selenium-ferroferric oxide (Se-Fe(3)O(4)) nanocomposites on human osteoblast-like MG-63 cells. The nearly spherical Se-Fe(3)O(4) nanocomposites of 40-50nm diameter, as characterized by transmission electron microscopy (TEM), are synthesized through reduction of selenious ...
Draux Florence - - 2009
Identifying early cellular events in response to a chemotherapy drug treatment, in particular at low doses that will destroy the highest possible number of cancer cells, is an important issue in patient management. In this study, we employed Fourier transform infrared spectroscopy as a potential tool to access such information. ...
Feyerabend Frank - - 2010
Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REEs) are used to improve the mechanical properties of the alloys, but in more or less undefined mixtures. For some elements of this group, data on toxicity and influence on cells are sparse. ...
Koulaouzidou Elisabeth A - - 2009
PURPOSE: To comparatively evaluate the effects of three dual activated adhesive resin cements on cell proliferation of rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC5). METHODS: The cements tested were RelyX ARC, RelyX Unicem and Panavia F. The cements were prepared according to manufacturers' instructions and placed in contact ...
Feng Tao - - 2009
Eight new monoterpenoid indole alkaloids, alstoyunines A-H (1-8), along with 17 known analogues, were isolated from Alstonia yunnanensis. The structures of the new alkaloids were established by means of extensive spectroscopic methods. Alstoyunines C (3), E (5), and F (6) showed selective inhibition of Cox-2 (>75%). Alstoyunine F (6) showed ...
Lin Rong-Jyh - - 2009
Three new butanolides, tenuifolide A (1), isotenuifolide A (2), and tenuifolide B (3), a new secobutanolide, secotenuifolide A (4), and one new sesquiterpenoid, tenuifolin (5), along with 16 known compounds were isolated from the stems of Cinnamomum tenuifolium. Their structures were determined by spectroscopic analyses. Compound 4 was found to ...
Kmetic Ivana - - 2009
Lindane, a toxic insecticide from the persistent organic pollutants (POP's) group, may act as an endocrine disrupter affecting crucial tissues of reproductive system. In this study a Chinese Hamster Ovary cell line (CHO-K1) was applied to assess the potential of lindane cytotoxicity at the cellular level. The methods of Trypan ...
Ishihara Mariko - - 2009
We recently demonstrated that the cytotoxicity of nineteen 1,2,3,4-tetrahydroisoquinoline derivatives depends on the molecular size (surface area, volume, width measured at 3-dimensional configuration), but not on most of the other electronic factors (Ishihara et al., Anticancer Res 29: 2265-2272, 2009). However, the information regarding cytotoxicity and molecular size in these ...
Ning Xuanxuan - - 2009
Discovery and development of new pharmaceuticals from marine organisms are attracting increasing interest. Several agents derived from marine organisms are under preclinical and clinical evaluation as potential anti-cancer drugs. We extracted and purified a novel anti-tumor protein from the coelomic fluid of Meretrix meretrix Linnaeus by ammonium sulphate fractionation, ion ...
Lu Zhenyu - - 2009
Eight drimane sesquiterpenes (1-8), six isochromane derivatives (9-14), and three known compounds, daldinin B (15), 9alpha-hydroxy-6beta-[(2E,4E,6E)-octa-2,4,6-trienoyloxy]-5alpha-drim-7-en-11,12-olide (16), and pergillin (17), were isolated from the EtOAc extract of the marine-derived fungus Aspergillus ustus 094102. The structures of the new compounds were elucidated on the basis of spectroscopic analysis. The cytotoxic effects ...
Lawnicka H - - 2009
The resorcylic acid lactone L-783,277, isolated from a Phoma sp. (ATCC 74403), is a potent and specific inhibitor of MEK (Map kinase kinase) that exerts very interesting pharmacological activities including anti-neoplastic properties. However, the role of this compound in the regulation of endocrine-related cancer cell growth and tumor progression remains ...
Misiek Mathias - - 2009
We report on the structure elucidation of arnamial, a new Delta(2,4)-protoilludane everninate ester from the fungus Armillaria mellea, and on the apoptotic activity of arnamial as well as the cytotoxic activity of structurally related compounds on selected human cancer cells. Arnamial showed cytotoxicity against Jurkat T cells, MCF-7 breast adenocarcinoma, ...
Nobre H?lio Vitoriano HV - - 2010
In this study, the cytoprotective effects of caffeine (CAF) and 8-(3-chlorostyryl)-caffeine (CSC), A(2A) receptor antagonists, were tested against 6-OHDA-induced cytotoxicity, in rat mesencephalic cells. Both drugs significantly increased the number of viable cells, after their exposure to 6-OHDA, as measured by the MTT assay. While nitrite levels in the cells ...
Nzaramba M Ndambe - - 2009
Some tuber-bearing wild potato species are reportedly higher in potential health-promoting traits, such as antioxidant activity (AOA) and total phenolic content (TP), than commercial cultivars; therefore, they could be used as parental material in breeding for high AOA and TP. However, using wild species might result in progenies that are ...
Hildebrand Heike - - 2010
Palladium/magnetite nanoparticulate catalysts were developed for efficient elimination of halogenated organic pollutants from contaminated wastewater. Particle recovery from treated water can be ensured via magnetic separation. However, in worst-case scenarios, this catalyst removal step might fail, leading to particle release into the environment. Therefore, a toxicological study was conducted to ...
Rana Indrajeetsinh - - 2010
Cytotoxicity of the two pesticides, thiram and endosulfan, have been studied in Ehrlich ascites tumor cells. Thiram cytotoxicity was much lower than that of endosulfan with LC(50) (1h exposure) of 4.02 and 1.12mM, respectively. The cytotoxic action of the pesticides on the cells were characterised by glutathione depletion, induction of ...
Houck Keith A - - 2009
The complexity of human biology has made prediction of health effects as a consequence of exposure to environmental chemicals especially challenging. Complex cell systems, such as the Biologically Multiplexed Activity Profiling (BioMAP) primary, human, cell-based disease models, leverage cellular regulatory networks to detect and distinguish chemicals with a broad range ...
Smolarz Katarzyna - - 2009
The world's largest brackish water sea area, the Baltic Sea, is considered to be one of the most polluted seas of the world. Many new pollutants are constantly entering the environment, such as brominated flame-retardants (BFRs). BFRs represent a group of compounds that structurally resemble hydrophobic organic contaminants, but only ...
Gualtieri Maurizio - - 2010
Air pollution in Milan causes health concern due to the high concentrations of particulate matter (PM10 and PM2.5). The aim of this study was to investigate possible seasonal differences in PM10 and PM2.5 chemical composition and their biological effects on pro-inflammatory cytokine release and cytotoxicity. The PM was sampled during ...
Tseng Chih-Hua - - 2009
A number of 6-arylindeno[1,2-c]quinoline derivatives were synthesized and evaluated for their antiproliferative activities against the growth of five cancer cell lines including human hepatocelluar carcinoma (Hep G2, Hep 3B and Hep2.2.1), non-small cell lung cancer (A549 and H1299), and normal diploid embryonic lung cell line (MRC-5). The preliminary results indicated ...
Dopp E - - 2010
Arsenic is a known human carcinogen, inducing tumors of the skin, urinary bladder, liver and lung. Inorganic arsenic, existing in highly toxic trivalent and significantly less toxic pentavalent forms, is methylated to mono- and di-methylated species mainly in the liver. Due to the low toxicity of pentavalent methylated species, methylation ...
Stratton Thomas R - - 2009
Quaternized copolymers of 4-vinylpyridine and poly(ethylene glycol) methyl ether methacrylate are known to have antibacterial properties and have displayed biocompatibility in red blood cell hemolysis assays. The results from hemolysis assays have shown substantial promise, but the technique is rudimentary and only a first step toward the determination of biocompatibility. ...
Rüweler Milena - - 2009
Resveratrol (3,4',5-trans-trihydroxystilbene) and other hydroxystilbenes exhibit in vitro antioxidant as well as prooxidant effects. The antioxidant properties are assumed to enable these compounds to protect cells from oxidative damage. The prooxidant effects are held likely to be responsible for their cytotoxic, anti-proliferative or pro-apoptotic effects observed in vitro. Regarding antioxidant/prooxidant ...
Harris W - - 2009
The main aim of this study was to determine whether sub-lethal concentrations of the organophosphate compound phenyl saligenin phosphate (PSP) could disrupt the activity of the Ca(2+)-activated enzyme tissue transglutaminase (TGase 2) from cultured cell lines of neuronal (N2a) and hepatic (HepG2) origin. The results indicated that PSP added directly ...
Silveira-Lacerda Elisângela de Paula Ede - - 2010
The aim of present study was to verify the in vitro antitumor activity of a ruthenium complex, cis-(dichloro)tetraammineruthenium(III) chloride (cis-[RuCl(2)(NH(3))(4)]Cl) toward different tumor cell lines. The antitumor studies showed that ruthenium(III) complex presents a relevant cytotoxic activity against murine B cell lymphoma (A-20), murine ascitic sarcoma 180 (S-180), human breast ...
Johnston Nikki - - 2009
OBJECTIVES: Studies using combined multichannel intraluminal impedance with pH monitoring reveal a role for nonacidic reflux in laryngopharyngeal symptoms and injury. We have discovered that pepsin is taken up by laryngeal epithelial cells by receptor-mediated endocytosis. This finding reveals a novel mechanism by which pepsin could cause cell damage, potentially ...
Sepet Elif - - 2009
OBJECTIVES: To examine the cytotoxic effects of MTA and Ca(OH)2 on 3T3 fibroblasts at different time intervals. METHODS: Confluent cells were cultured with Ca(OH)2 and MTA in six-well plates. Wells with only fibroblasts served as controls. Cell number and viability were determined after 24 and 48 hours and 7 days ...
Knauer Sebastian - - 2009
The unfavorable therapeutic index of the fungal cytotoxin illudin M was to be improved by covalent attachment of the redox modulator and phenyl isobiostere ferrocene. Esters of illudin M with ferrocenoic and 1,1'-ferrocenedioic acid were prepared, structurally characterised (X-ray), and tested for cytotoxicity [MTT assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], induction of apoptosis ...
Awale Suresh - - 2009
The 70% ethanol extract of Soymida febrifuga was found to kill PANC-1 human pancreatic cancer cells preferentially under nutrition-deprived conditions at a concentration of 10 microg/mL. Phytochemical investigation led to the isolation of 27 compounds including four new compounds [(3R)-6,4'-dihydroxy-8-methoxyhomoisoflavan (1), (2R)-7,4'-dihydroxy-5-methoxy-8-methylflavan (2), 7-hydroxy-6-methoxy-3-(4'-hydroxybenzyl)coumarin (3), and 6-hydroxy-7-methoxy-3-(4'-hydroxybenzyl)coumarin (4)]. 2',4'-Dihydroxychalcone (8) ...
Yao Yao - - 2009
Two new piperazine-2,5-dione derivatives, gliocladride A (1) and B (2), along with deoxymycelianamide (3) were isolated from the mycelial mass of marine fungus Gliocladium sp. Their structures were established on the basis of spectral data, and the stereochemical assignments were made by chiral HPLC analysis of the hydrolyzed compounds and ...
Correa Gabriela Tavares Bekner - - 2009
The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of ...
Liu Hongbing - - 2009
Seven new drimane sesquiterpenoids (1-3, 6-9), along with the known compounds deoxyuvidin B (4), strobilactone B (5), and RES-1149-2 (10), were obtained from cultures of the fungus Aspergillus ustus, which was isolated from the marine sponge Suberites domuncula. Their structures were established by means of spectroscopic analyses including one- and ...
Harorli Osman-Tolga - - 2009
OBJECTIVE: Resin based dental materials are not stable in the oral environment and may release their components into biological media. These components may include substances such as triethylene glycol dimethacrylate (TEGDMA), which is a major co-monomer of dental resin materials. This release can trigger host immune and inflammatory responses against ...
R?ti Andrea - - 2009
The antiproliferative effect of 5-fluorouracil (5-FU) in the presence of low dose non-steroidal anti-inflammatory drugs (NSAIDs) on high cyclooxygenase-2 (COX-2)-expressing HCA-7 and low COX-2-expressing HT-29 colon carcinoma cell lines was investigated. Pharmacogenetic parameters were studied to characterize the 5-FU sensitivity of the two cell lines. Thymidylate synthase (TS) and methylenetetrahydrofolate ...
Durand Grégory - - 2009
Nitrones have been employed as spin trapping reagent as well as pharmacological agent against neurodegenerative diseases and ischemia-reperfusion induced injury. The structure-activity relationship was explored for the two types of nitrones, i.e., cyclic (DMPO) and linear (PBN), which are conjugated to a fluorinated amphiphilic carrier (FAC) for their cytoprotective properties ...
Pledgie-Tracy Allison - - 2010
Polyamine analogues have demonstrated significant activity against human breast cancer cell lines as single agents as well as in combination with other cytotoxic drugs. This study evaluates the ability of a polyamine analogue N (1),N (11)-bis(ethyl)norspermine (BENSpm) to synergize with six standard chemotherapeutic agents, 5-fluorouracil (FU), fluorodeoxyuridine, cis-diaminechloroplatinum(II) (C-DDP), paclitaxel, ...
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