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Results 301 - 350 of 1346
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Kim In Sik - - 2007
Hottuynia cordata Thunb (Saururaceae; HC) is known as a therapeutic drug that has been used in traditional oriental medicine for the treatment of allergy. Mast cells play an important role in a variety of inflammatory diseases, and specifically asthma and atopy. In the present study, we investigated the effect of ...
Gupta, S K
Saccharomyces cerevisiae cells were immobilized on three supports—calcium alginate beads, raw and hexamethylene diamine treated bagasse—for their comparative assesment in terms of cell retention on to the carrier and metabolic and physiological activities of the immobilized cells. The chosen carrier-activated bagasse adsorbed 0.41 g of cells per g of carrier ...
Cunha M A A - - 2007
Xylose-to-xylitol conversion was investigated in a bench-scale bioreactor using Candida guilliermondii cells entrapped within polyvinyl alcohol-hydrogel beads in a system operated in repeated-batch mode with cell recycling. Yeast-viable cells were immobilized in the support using the freezing-thawing method. Bioconversion assays were performed in a stirred tank reactor operated at 400-rpm ...
Bock, A.
The use of a perfusion system prevented unspecific inhibitions effects on cell growth for batch cultivations of adherent MDCK cells used for an equine influenza A vaccine production process. It also increased viable cell numbers. An enzyme sensor for monitoring and closed loop control of metabolites operated stable for at ...
Mandoc, M.M.
In organic semiconductors the recombination mechanism is of the Langevin type, controlled by the mobility of the charge carriers. As a result, in organic solar cells the mobility simultaneously controls both the carrier extraction and the losses via carrier recombination. The authors demonstrate that the balance between carrier losses by ...
Breguet, Véronique
Since life began, nature has been using the envelopment of systems for their protection or for providing a particular reaction space, the enveloping wall displaying additional specific membrane functions. The abundance of examples is quite overwhelming and extends from arboreal fruits and plant seeds to plant seed spores, from the ...
Wu Cheng - - 2007
The use of cell-free systems enables biochemical determination of factors and mechanisms contributing to translational processes. The preparation and use of cell-free translation systems from the fungi Saccharomyces cerevisiae and Neurospora crassa are described. Examples provided illustrate the use of these systems, in conjunction with luciferase assays, [(35)S]Met incorporation, and ...
Smeaton Michael B - - 2007
The repair of DNA by nucleotide excision repair (NER) and non-homologous end joining (NHEJ) is essential for maintenance of genomic integrity and cell viability. Examination of NHEJ and NER in vitro using cell-free extracts has led to a deeper understanding of the biochemical mechanisms that underlie these processes. Current methods ...
Wei Shenghua - - 2007
Dihydroxyacetone (DHA) is of great interest in the fine chemical and pharmaceutical industry; therefore, the discovery of suitable biocatalysts for the efficient production of it is very necessary. In the experiment, Gluconobacter oxydans was immobilized in polyvinyl alcohol (PVA). Various parameters of the immobilized cells were investigated. The results have ...
Chen C Y - - 2007
Cyanide is highly toxic to living organisms, particularly in inactivating the respiration system by tightly binding to terminal oxidase. To protect the environment and water bodies, wastewater containing cyanide must be treated before discharging into the environment. Biological treatment is a cost-effective and environmentally acceptable method for cyanide removal compared ...
Sheeja K - - 2007
The stimulatory effect of Andrographis paniculata extract and andrographolide on cytotoxic T lymphocyte (CTL) production was determined in BALB/c mice by Winn's neutralization assay using CTL sensitive EL4 thymoma cells as target cell. Extract and andrographolide showed a significant increase in CTL production in both the in vivo and in ...
Khaw Teik Seong - - 2007
The specific ethanol production rate of raw starch by arming yeast cells displaying alpha-amylase and glucoamylase increased significantly when the cells and starch granules settled together. The specific ethanol production rate also increased when the size distribution of starch granules was almost same as that of the yeast cells. These ...
Weber Christian - - 2007
The use of commercially available plastic syringes is introduced as disposable small-scale fixed bed bioreactors for the cultivation of implantable therapeutic cell systems on the basis of an alginate-encapsulated human mesenchymal stem cell line. The system introduced is fitted with a noninvasive oxygen sensor for the continuous monitoring of the ...
Bouallagui Zouhaier - - 2006
An immobilized whole cell system was successfully performed to produce the most powerful antioxidant, hydroxytyrosol. Bioconversion of tyrosol into hydroxytyrosol was achieved via the immobilization of Pseudomonas aeruginosa resting cells in calcium alginate beads. Immobilization was advantageous as it allows immobilized cells to tolerate a greater tyrosol concentration than free ...
Hughson, Jason D.
Presented here is an investigation and comparison of two entrapment methods of algae. Conventional calcium alginate bead entrapment is compared to a recently described modified bead entrapment method, utilizing poly(ethylene glycol) and calcium alginate, which results in void or capillary formation within the beads. It is shown that algal growth ...
Park Jung-Soon - - 2007
A self-assembled multilayer (SAMu) including the alginate layer was prepared for detecting Pseudomonas aeruginosa cells in a solution and its potential was evaluated with a BIAcore system. After layer-by-layer formation, the refractive units (RU) values monitored with the biosensor increased by the interaction between the layers. The responses by the ...
Choi J W - - 2007
The immobilized cell using self-assembled synthetic oligopeptide was applied to the electrochemical detection of pathogen infection. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for the immobilization of HeLa cell on gold (Au) substrate. Layer formation and immobilization of the cell were investigated with surface plasmon resonance (SPR) and ...
Khare, S K
Aspergillus oryzae cells were entrapped within agarose beads. These entrapped cells, having α-galactosidase activity of 0.72 IU/ml, were used for hydrolyzing flatulence-causing galacto-oligosaccharides present in soymilk. The total of 52% raffinose, 70% stachyose, and 66.3% total oligosaccharides of soymilk was observed to be hydrolyzed in 8 h at 50°C by ...
Moreno-Garrido I - - 2007
Populations of calcium-alginate immobilized marine diatom Phaeodactylum tricornutum were exposed to two sediments containing different levels of surfactant (LAS). Toxic responses were compared for free and immobilized algae. Although there is a direct relation between LAS content in sediment and inhibition, immobilized algae suffered less inhibition than free cells, over ...
Mizunuma Takato - - 2007
We studied the immobilization of a mycelium (Aspergillus niger) using the working hypothesis as follows: (a) when polycation was added to the cell suspension, a few parts of it would bind on the surface of a hypha, allowing to gather the hyphae in part but not all; (b) upon further ...
Mundra Piyushkumar - - 2007
Response surface methodology (RSM), based on multivariate non-linear model, was applied to study the interactions and optimization of the immobilization parameters for cell entrapment, namely alginate concentration, cell loading and bead diameter using Erwinia rhapontici NCPPB 1578 that produced palatinose. ANOVA analysis and statistical parameters calculations showed that RSM could ...
Ezure Toru - - 2006
We established a novel cell-free protein synthesis system derived from Trichoplusia ni (HighFive) insect cells by a simple extraction method. Luciferase and beta-galactosidase were synthesized in this system with active forms. We analyzed and optimized (1) the preparation method of the insect cell extract, (2) the concentration of the reaction ...
Hart, Kimberly Ann, 1974-
In north central Texas, USA, free-water surface wetlands have been constructed to treat pre-treated wastewater effluent from the Trinity River. Water quality and vegetation data from the first two years of operation (June 2003 to May 2005) were used to determine cell-to-cell and system-wide removal efficiency of total suspended solids ...
Sarada R - - 2006
Haematococcus pluvialis is one of the potent organisms for production of astaxanthin, a high value ketocarotenoid. Astaxanthin is accumulated in thick-walled cyst cells of Haematococcus. The thick cell wall is made up of sporopollenin-like material, algaenan, which hinders solvent extraction of astaxanthin. In the present study, an improved method for ...
Wang, Henry Y.
Many antibiotic fermentations are sensitive to high concentrations of their own product possibly due to product regulation and toxicity mechanisms. In this paper we discuss the feasibility of using affinity adsorption with biospecific ligands for in situ product removal to alleviate this problem. The concept of using whole cells containing ...
Chau Andrew S AS Schering-Plough Research Institute, 2015 Galloping Hill Road, 4700, Kenilworth, NJ 07033, - - 2006
Posaconazole and itraconazole were more potent inhibitors of ergosterol synthesis, in both intact cells and cell extracts from Absidia corymbifera and Rhizopus oryzae, than voriconazole and fluconazole. Similarly, expression of CYP51 from R. oryzae in Saccharomyces cerevisiae significantly increased resistance to fluconazole and voriconazole but not to posaconazole and itraconazole.
Yoo Sung Keun - - 2007
A bio-MEMS based cell-chip that can detect a specific toxicity was fabricated by patterning and immobilizing bioluminescent bacteria in a microfluidic chip. Since the emitted light intensity of bioluminescent bacteria changed in response to the presence of chemicals, the bacteria were used as the toxicity indicator in this study. A ...
Baruch Limor - - 2006
The use of chitosan in complexation with alginate appears to be a promising strategy for cell microencapsulation, due to the biocompatibility of both polymers and the high mechanical properties attributed by the use of chitosan. The present work focuses on the optimization and characterization of the alginate-chitosan system to achieve ...
Ibrahim, H; Department of ...
<i>Terminalia macroptera</i> Guill. & Perr. (Combretaceae) is used for stomach ailments and skin diseases. Macroscopical, microscopical and quantitative evaluation of the leaves, whole and powdered was carried out. Microscopic features include the following parenchyma cells, stomata (anisocytic, anomocytic, actionocytic), trichomes (uniseriate, multicellular 2-4 cells), rosettes or prism of calcium oxalate ...
Verbelen Pieter J - - 2006
In several yeast-related industries, continuous fermentation systems offer important economical advantages in comparison with traditional systems. Fermentation rates are significantly improved, especially when continuous fermentation is combined with cell immobilization techniques to increase the yeast concentration in the fermentor. Hence the technique holds a great promise for the efficient production ...
Perullini Mercedes - - 2007
In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as calcium-alginate, cannot fully protect entrapped cells. ...
Landwehr Jens - - 2006
Compounds that inhibit 5-lipoxygenase (5-LO), the key enzyme in the biosynthesis of leukotrienes (LTs), possess potential for the treatment of inflammatory and allergic diseases as well as of atherosclerosis and cancer. Here we present the design and the synthesis of a series of novel 2-amino-5-hydroxyindoles that potently inhibit isolated human ...
Lebleu Valerie S - - 2006
A culture system for performing cell cycle analyses on cells in undisturbed steady-state populations was designed and tested. In this system, newborn cells are shed continuously from an immobilized, perfused culture rotating about the horizontal axis. As a result of this arrangement, the number of newborn cells released into the ...
Mikami Satoshi - - 2006
Since a large number of eukaryotic proteins are glycoproteins, an efficient and easily available cell-free system for the production of recombinant glycoproteins is needed. We have successfully developed an efficient cell-free translation system derived from a monoclonal antibody-producing hybridoma for this purpose. While extracts from HeLa cells were very inefficient ...
Campbell Derek W - - 2006
There is a significant need for devices capable of measuring water contaminant concentrations in situ--continuously, rapidly, and without reagents, extraction, or other pretreatment. Toward this goal, we constructed and tested fiber optic biosensors for measurement of 1,2-dichloroethane (DCA) in aqueous solutions. The biocomponent was the haloalkane dehalogenase, DhlA, in whole ...
Kim Tae-Wan - - 2006
In this study, as a part of our efforts to improve the robustness and economical feasibility of cell-free protein synthesis, we developed a simple method of preparing the cell extracts used for catalyzing cell-free protein synthesis reactions. We found that the high-speed centrifugation, pre-incubation, and dialysis steps of the conventional ...
Oh In Kyung - - 2006
The surface of adenovirus (ADV) was modified with folate-poly(ethylene glycol) (FOL-PEG) conjugate to overcome the inherent problems associated with viral gene therapy. The ADV coding the green fluorescent protein (GFP) gene was used to immobilize PEG or FOL-PEG conjugate on the viral surface to comparatively evaluate their extents of retargeting ...
Katzen Federico - - 2006
A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is ...
Lin, Jian-Er
Non-immobilized, immobilized and co-immobilized Arthrobacter (ATCC 33790) cells were examined for their ability to degrade PCP in a mineral medium. Non-immobilized cells could completely remove PCP from the aqueous phase and mineralize 77% of the added PCP within 135 h. Alginate-encapsulated cells mineralized 86% of the PCP with a similar ...
Swain M R - - 2007
There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in fermentable ...
Stano Ján - - 2006
Permeabilized tomato cells were cross-linked with glutaraldehyde in the absence of a carrier. The immobilized cells demonstrated significantly lower aminopeptidase (AP) activities than untreated control cells. However, when immobilized with pectate and alginate gels, the tomato cells retained their AP activities. A new method for the determination of the activity ...
Cheng Jing-Sheng - - 2006
A proteomic approach was used to study the responses of Taxus cuspidata cells to local microenvironments in different zones of immobilized support matrices. Analysis of protein spots by 2-DE revealed significant differences in the abundance of 31 spots, 28 spots, and 23 spots in outer, middle, and central zone cells ...
Efremenko E - - 2006
Spores of the filamentous fungus Rhizopus oryzae were entrapped in macroporous poly(vinyl alcohol) cryogel (PVA-cryogel). To prepare immobilised biocatalyst capable of producing L(+)-lactic acid (LA), the fungus cells were cultivated inside the carrier beads. The growth parameters and metabolic activity of the suspended (free) and immobilised cells producing LA in ...
Meuwly F - - 2006
Packed-bed bioreactors (PBR) have proven to be efficient systems to culture mammalian cells at very high cell density in perfusion mode, thus leading to very high volumetric productivity. However, the immobilized cells must be continuously supplied with all nutrients in sufficient quantities to remain viable and productive over the full ...
Adams Lynn S - - 2006
Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability. We then modeled the interactions between botanical extracts in combination isobolographically. ...
Xie J T - - 2006
AIM: Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we investigated the effects of Ganoderma lucidum ...
Silva Ana Isabel - - 2006
This paper presents the concept and most of the research undertaken all over the world for the development of a bio-artificial pancreas (BAP) device over the last 30 years. The devices studied, meant to mimic the insulin secretion of the natural organ, were diverse and have been reviewed. Allogeneic or ...
DeSousa Sandro Rogério - - 2006
Separation of microbial cells by flotation recovery is usually carried out in industrial reactors or wastewater treatment systems, which contain a complex mixture of microbial nutrients and excretion products. In the present study, the separation of yeast cells by flotation recovery was carried out using a simple flotation recovery systems ...
SAKAMOTO, Tsukasa
The in vitro cultivation of the germinal cells from daughter cyst of Echinococcus multilocularis was investigated. The medium consisted essentially of equivalent HANKS' balanced salt solution with 0.5% lactalbumin and 0.1% yeast extract and medium 199 to which 20% calf serum was added. The results obtained are as follows. The ...
Olsson Marie E - - 2006
The effects of extracts from five cultivars of strawberries on the proliferation of colon cancer cells HT29 and breast cancer cells MCF-7 were investigated, and possible correlations with the levels of several antioxidants were analyzed. In addition, the effects of organic cultivation compared to conventional cultivation on the content of ...
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