Search Results
Results 401 - 450 of 1877
< 4 5 6 7 8 9 10 11 12 13 14 >
Cushnie T P T - - 2007
AIM: The flavonol galangin, an antimicrobial constituent of the traditional medicines propolis and Helichrysum aureonitens, is being assessed as part of an ongoing investigation into the antibacterial activity of flavonoids. The present study sought to establish whether galangin has any aggregatory effect on bacterial cells. METHODS AND RESULTS: In preparatory ...
Kitts David D - - 2007
Pure ginsenoside standards (saponins Rh2, PD, and PT), along with an Rh2-enhanced North American ginseng (Panax quinquefolius) leaf extract (LFRh2), were tested for cytotoxic activity in cultured THP-1 leukemia cells. Thermal treatment of ginseng leaf resulted in production of both Rh2 and Rg3 content that was confirmed by liquid chromatography ...
aalster and tzohary
This is probably the most abundant very small (3-5 um diameter) single-celled species in the lake, at times reaching 3000-5000 cells/ml. Erkenia can occur in the water at all times of the year although it tends to be more abundant in winter. In addition to the small cell size and ...
Kawahara Shuhei - - 2007
PURPOSE: To investigate the intracellular events in retinal glial cells exposed to indocyanine green (ICG) and brilliant blue G (BBG). METHODS: The human Müller cell line MIO-M1 was exposed to a low dose (0.25 mg/mL) and a clinical dose (2.5 mg/mL) of ICG and a clinical dose (0.25 mg/mL) of ...
Nascimento Juliana Minardi - - 2007
Bothrops snake venoms cause renal damage, with renal failure being the main cause of death in humans bitten by these snakes. In this work, we investigated the cytoskeletal rearrangement and cytotoxicity caused by Bothrops alternatus venom in cultured Madin-Darby canine kidney (MDCK) cells. Incubation with venom (10 and 100 microg/mL) ...
aalster and tzohary
This small (cells: 4-9.5um) chlorophyte, is usually rare in the plankton of Lake Kinneret, but formed significant blooms exceeding 1000 cells/mL in winter 1997 and again in winter 2000. The cells are triangular with one rounded side and organized in fours so that each coenobium of 4 triangles forms a ...
aalster and tzohary
This is the most abundant and common pennate diatom of Lake Kinneret. The species was first described in 1996 based on material from Lake Kinneret, to the best of our knowledge until today is was not reported to occur anywhere else. It appears in many morphotypes, hence its name. It ...
Heiser Philip - - 2007
Cytotoxic effects on neuronal as well as on immune cells have been reported for both typical and atypical antipsychotic drugs. We evaluated the effects of different concentrations of a typical (haloperidol) and two atypical (clozapine, olanzapine) antipsychotics on the survival of human neuronal (SH-SY5Y cells) and immune cells (U937 cells) ...
Antoci Valentin V - - 2007
Antibiotic concentrations associated with antibiotic bone cements may cause skeletal cell toxicity and prevent fracture healing. We investigated toxicity effects of dose and treatment time after exposure to three antibiotics commonly used in orthopaedic local drug delivery systems. We hypothesized a threshold exists for toxicity of osteoblasts and chondrocytes after ...
Urbanska Aleksandra Malgorzata - - 2007
Targeted delivery of live microencapsulated bacterial cells has strong potential for application in treating various diseases, including diarrhea, kidney failure, liver failure, and high cholesterol, among others. This study investigates the potential of microcapsules composed of two natural polymers, alginate and chitosan (AC), and the use of these artificial cells ...
Simpkins Cuthbert O - - 2007
BACKGROUND: We previously demonstrated that the degradation of a suspension of Jurkat cells in Ringer's lactate (RL) was inhibited by the addition of a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid/Tris buffer. Given the ability of histidine to buffer protons in the physiologic range (pKa = 6.0), we hypothesized that this amino acid would have ...
Dutta Avijit - - 2007
Leishmaniasis remains a major health problem of the tropical and subtropical world. The visceral form causes the most fatalities if left untreated. Dramatic increases in the rates of infection and drug resistance and the non-availability of safe vaccines have highlighted the need for identification of novel and inexpensive anti-leishmanial agents. ...
Dharmu Indra - - 2007
The hemolymph-derived achatinin(H) (lectin) from Achatina fulica showed a marked cytotoxic effect on MCF7, a human mammary carcinoma cell line. IC(50) values as measured by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay for achatinin(H) ranged from 6 to 10 microg/ml in the MCF7 cells. MCF7 cells showed significant morphological changes leading to cell ...
Ogola Henry - - 2007
In this study, we investigated the impact of mastitis infection on the quality of milk composition in small-scale dairy bovine herds. The purpose of this study was to find a milk quality somatic cell count (SCC) standard that could be used as an integral component of a control program. In ...
Moore A B - - 2007
Due to dietary exposure of women to genistein, a soy-derived phytoestrogen, and the estrogen responsiveness of uterine leiomyomas 'fibroids', we evaluated the effects of genistein (0.001-50 microg/ml) on human uterine leiomyoma (UtLM) cells versus uterine smooth muscle cells (UtSMCs) in vitro. Light microscopy was used to determine the effects of ...
Sadik, K.
The improvement of East African highland bananas (EAHBs) using conventional breeding methods is difficult due to their biology and therefore focus on improving them has shifted to exploring methods for establishment of embryogenic cell suspensions, which can then be targeted, for genetic transformation. Shoots of four cultivars namely 'Musakala', 'Kibuzi', ...
Corona Giulia G Molecular Nutrition Group, School of Chemistry, Food and Pharmacy, University of Reading, Whiteknights Campus, Reading RG2 6AP, - - 2007
We investigated the anti-proliferative effects of an olive oil polyphenolic extract on human colon adenocarcinoma cells. Analysis indicated that the extract contained hydroxytyrosol, tyrosol and the various secoiridoid derivatives, including oleuropein. This extract exerted a strong inhibitory effect on cancer cell proliferation, which was linked to the induction of a ...
Ohkuma, Taeko
Effeets of isoprothiolane (IPT : diisopropyl 1,3-dithiolane-2-ylidenemalonate)on hyphal mass of <special>Pyricularia oryzae</special> Cav. were investigated. Preliminary to the exploration of the IPT effects, the IPT-tolerant isolate, designated as PI13, was obtained through selection of a tolerant colony which had originated from a singlele protoplast derived from the IPT-sensitve isolate P2. ...
He Weihong - - 2008
Polybrominated diphenyl ethers (PBDEs) are an important class of flame retardants. Because of their detection in human breast milk and structural similarity to polychlorinated biphenyls (PCBs), concern has been raised about their potential toxicity, particularly neurotoxic effects in newborns and children. The aim of the current study was to evaluate ...
Oh Jaeryung - - 2007
PURPOSE: We investigated the effects of short-term exposure to triamcinolone on cultured choroidal fibroblast (CFB) cells and retinal pigment epithelial (RPE) cells. METHODS: To evaluate the effect of triamcinolone on cell proliferation, CFB and RPE cells were divided into three groups: a short-term exposure group; a longterm exposure group, and ...
Kogoshi Hanae - - 2007
gamma-Secretase inhibitors (GSI) suppress the growth of acute T-lymphoblastic leukemia (T-ALL) cells with NOTCH1 mutations. Recently, clinical trials of GSI for refractory T-ALL have commenced. In the present study, we examined the effects of three types of GSI; GSI-I, GSI-IX, and GSI-XII on the growth of four B-cell malignant lymphoma ...
Banfalvi Gaspar - - 2007
Murine pre-B-cells grown in the presence of lower (1 microM) or higher (5 microM) concentration of cadmium chloride were separated into 13 fractions by centrifugal elutriation. The rate of DNA synthesis after cadmium treatment determined in permeable cells was dependent on cell culture density during cadmium treatment. Cell cycle analysis ...
Lyew Joelle - - 2007
Spatial features of pollen tube growth and the composition of the extracellular matrix (ECM) of transmitting tissue in carpels of Kadsura longipedunculata, a member of the basal angiosperm taxon Schisandraceae, were characterized to identify features of transmitting tissue that might have been important for pollen-carpel interactions during the early history ...
Lawuyi Bisi - - 2007
This article demonstrates the potential of encapsulated, engineered Lactococcus lactis as a vehicle for the oral delivery of therapeutic proteins. Using alginate-poly-l-lysine-alginate membrane-encapsulated L. lactis engineered to secrete the reporter protein Staphylococcal aureus nuclease, we show comparable viability and protein secretion between free and immobilized cells. After 12 h, microcapsules ...
Gajda Barbara - - 2007
The aim of the experiment was to investigate the effect of vitrification on viability and the cell cycle of bovine cumulus cells and fibroblasts after culture with or without serum starvation. In all vitrified-thawed bovine somatic cells, the number of samples that reached the confluence stage was high (50 to ...
Sierosławska A - - 2007
The in vitro influence of microcystin-LR (MC-LR) on rainbow trout phagocytic cell functions was studied. The cells isolated from the fish blood were exposed on MC-LR at the concentrations of 1, 5, 10, 20 microg/mL. The viability of the cells after 2, 4 and 24 hour incubation with MC-LR was ...
Troken A - - 2007
The ultimate goal in the tissue engineering of the synovial joint is to fabricate biologically derived analogues that can replace severely degenerated or traumatized synovial joint components. A number of challenges must be addressed before reaching this ultimate goal. In this report, the relevance of cell seeding density in the ...
Qin Zhiqiang - - 2007
Staphylococcus epidermidis has become a significant pathogen causing infections due to biofilm formation on surfaces of indwelling medical devices. Biofilm-associated bacteria exhibit enhanced resistance to many conventional antibiotics. It is therefore, important to design novel antimicrobial reagents targeting S. epidermidis biofilms. In a static chamber system, the bactericidal effect of ...
González Norma V - - 2007
The sister chromatid exchange (SCE) frequency, the cell-cycle progression analysis, and the single cell gel electrophoresis technique (SCGE, comet assay) were employed as genetic end-points to investigate the geno- and citotoxicity exerted by dicamba and one of its commercial formulation banvel (dicamba 57.71%) on Chinese hamster ovary (CHO) cells. Log-phase ...
Shibata Kaori - - 2007
The toxic effects of man-made mineral fibers (MMMFs) have been evaluated by cell magnetometry using alveolar macrophages (AMs). Recently, on the other hand, the murine macrophage cell line, RAW 264.7, became available and has been used as an in vitro model of AMs. The objective of this study was to ...
Jardin Barbara Ann - - 2007
The development of insect cells expressing recombinant proteins in a stable continuous manner is an attractive alternative to the BEV system for recombinant protein production. High cell density fed batch and continuous perfusion processes can be designed to maximize the productivity of stably transformed cells. A cell line (Sf-9SEAP) expressing ...
Sutton Dwayne J - - 2007
The underlying mechanism for the biological activity of inorganic mercury is believed to be the high affinity binding of divalent mercuric cations to thiols of sulfhydryl groups of proteins. A comprehensive analysis of published data indicates that inorganic mercury is one of the most environmentally abundant toxic metals, is a ...
Calabretta Michelle K MK Department of Chemistry, University of Houston, Houston, Texas 77204, - - 2007
Poly(amidoamine) (PAMAM) dendrimer derivatives have been investigated for their biological applications, especially for delivery of drugs, including antimicrobial drugs to eukaryotic cells, but their effects on bacterial cells are largely unexplored. Herein we report that amino-terminated PAMAM dendrimers are highly toxic to the common Gram-negative pathogen Pseudomonas aeruginosa. The concentration ...
CHIBA, Hitomi
We investigated the effects of epidermal growth factor (EGF) on cell migration, and the association of focal adhesion kinase (FAK) and paxillin to cytoskeleton. A cell line of squamous cell carcinoma (SCC) of the uterine cervix, named Caski, was used. We examined the effects of different concentrations of EGF (0, ...
Tokue, Takanori
The MeOH extract of a marine sponge of the genus Spongia showed neurotrophic activity in pheochromocytoma (PC-12) cells. Purification of the extract afforded a new sesterterpene, deacetoxyscalarin (1), along with five known sesterterpenes (2-6). Among them, 2-6 induced neurite outgrowth in PC-12 cells at the concentration of 50 μg/mL but ...
Wong S F - - 2007
Cell disruption or lysis is a crucial step to obtain cellular components for various biological studies. We subjected different concentrations of Candida albicans to 5, 10, 15 and 20 cycles of disruption. The degree of cell lysis was observed using light microscopy and the yields obtained were measured and analysed. ...
Vijaya Padma Viswanadha - - 2007
Ginger (Zingiber officinale Roscoe, Zingiberaceae) is a commonly used medicinal herb throughout the world. Although some studies have demonstrated its antitumour activities on cancer cells in vitro and in vivo, the exact mechanism is not fully elucidated. Hence, the present study was designed to examine the in vitro cytotoxic activities ...
Lund Kaleb C - - 2007
Nucleoside analogs are associated with various mitochondrial toxicities, and it is becoming increasingly difficult to accommodate these differences solely in the context of DNA polymerase gamma inhibition. Therefore, we examined the toxicities of zidovudine (AZT) (10 and 50 microM; 2.7 and 13.4 microg/ml), didanosine (ddI) (10 and 50 microM; 2.4 ...
Abranches Elsa - - 2007
Embryonic stem (ES) cells have been shown to differentiate in vitro into a wide variety of cell types having significant potential for tissue regeneration. Therefore, the operational conditions for the ex vivo expansion and differentiation should be optimized for large-scale cultures. The expansion of mouse ES cells has been evaluated ...
Rumampuk, Natalie D. C.
Toxic effects of triphenyltin (TPT) on the marine alga Eucheuma denticulatum obtained from Nain Island, North Sulawesi, Indonesia have been examined in laboratory condition. The algal samples were first acclimated in laboratory prepared seawater for three days. The algae were then divided into 12 culture chambers for treatments with different ...
Park Kwan Soon - - 2007
A fish pond ecosystem on the west coast of Korea was studied for biotic and abiotic factors. The occurrence rate of phytoplankton at the embanked fish farm was 84 times higher to that of shore waters. Phytoplankton species which occurred during the study period were categorized into Cyanophyta, Dinophyta and ...
Khlusov I A - - 2007
The effects of Xe on cell viability and redox balance in the culture of Wistar rat thymocytes were studied in vitro during 24-h storage under hypothermic conditions. The results indicate that after bubbling of cell suspensions (5 x 10(6) cell/ml, 4 ml medium), the weight of Xe in flasks was ...
Miller Adam M - - 2007
PURPOSE: To challenge human trabecular meshwork (TM) cells using lactate to mimic cell stress and observe the effects on cell viability, NF-kappaB, and membrane type 1 matrix metalloproteinase (MT1-MMP) expression and the ectodomain shedding of soluble (s)CD44. METHODS: Human TM cells grown in 10% fetal calf serum (FCS) were incubated ...
Srivastava Anita - - 2007
In recent years, considerable attention has been paid to anthocyanins due to their abilities to inhibit oxidative stress and cell proliferation. The regulations of apoptosis and the phase II enzymes glutathione-S-transferase (GST) and quinone reductase (QR) are other potential mechanisms through which flavonoids such as anthocyanins may prevent cancer. Our ...
Szurman Peter - - 2007
Epiretinal deposits of triamcinolone acetonide (TA) can be detrimental to retinal cells in vitro as several laboratory studies have shown. This contrasts with the good clinical experience of intravitreal TA use. We investigated the effect of TA crystals on retinal cells concerning the critical dose range, a potential cell recovery, ...
Okoli Arinze S - - 2007
Helicobacter hepaticus infects the bowel and biliary tree of several animals, producing inflammation. Colonisation of mouse livers can induce hepatocellular carcinomas. The effects of H. hepaticus on the proliferation and global protein expression of human HEp-2 cells were studied by examining the changes in the protein profiles of cells exposed ...
Spitzer Martin S - - 2007
To compare the antiproliferative and cytotoxic properties of bevacizumab (Avastin), pegaptanib (Macugen) and ranibizumab (Lucentis) on human retinal pigment epithelium (ARPE19) cells, rat retinal ganglion cells (RGC5) and pig choroidal endothelial cells (CEC). Monolayer cultures of ARPE19, RGC5 and CEC were used. Bevacizumab (0.1-0.3 mg/ml), pegaptanib (0.025-0.08 mg/ml) or ranibizumab ...
Al-Saeedi Fatma - - 2007
BACKGROUND: [Methyl-3H]-choline is a promising new positron emission tomography (PET) agent used for cancer imaging whose mechanism has still not fully been elucidated. In this study, whether [methyl-3H]-choline determined by measuring the activity of choline kinase (ChoK) and phospholipase D (PLD) in rapidly proliferating and confluent breast cancer MCF-7 cells ...
Hirasawa Hiroyo - - 2007
PURPOSE: To examine the intracellular uptake and extracellular binding of indocyanine green (ICG) and trypan blue (TB) by human retinal pigment epithelium (RPE) cells in vitro at clinically relevant concentrations. METHODS: Human RPE cells (ARPE19 cells) were exposed to 0.01%, 0.05%, and 0.25% ICG or 0.06%, 0.15%, and 0.25% TB ...
Klarić Maja Segvić - - 2007
Individual and combined effects of the mycotoxins fumonisin B(1), beauvericin and ochratoxin A on cell viability, lipid peroxidation (TBARS) and intracellular glutathione (GSH) were studied on porcine kidney epithelial cells (PK15). Cells were treated with 0.05, 0.5 and 5 microg/ml of each mycotoxin or the combinations of two or all ...
< 4 5 6 7 8 9 10 11 12 13 14 >