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Ma Xuefei X Laboratory of Molecular Cardiology; National Heart, Lung, and Blood Institute; National Institutes of Health; Bethesda, MD - - 2014
Three different genes each located on a different chromosome encode the heavy chains of nonmuscle myosin II in humans and mice. This review explores the functional consequences of the presence of three isoforms during embryonic development and beyond. The roles of the various isoforms in cell division, cell-cell adhesion, blood ...
Krüger Nadine N Institute of Virology, University of Veterinary Medicine Hannover, Hannover, - - 2014
Henipaviruses are associated with pteropodid reservoir hosts. The glycoproteins G and F of an African henipavirus (strain M74) have been reported to induce syncytium formation in kidney cells derived from a Hypsignathus monstrosus bat (HypNi/1.1), but not in the non-chiropteran cells BHK-21 and Vero76. Here we show that syncytia are ...
Pacheco Jorge M JM Departamento de Matemática e Aplicações , Universidade do Minho , Braga 4710-057 , Portugal ; Centro de Biologia Molecular e Ambiental , Universidade do Minho , Braga 4710-057 , Portugal ; ATP-Group, CMAF , Instituto para a Investigação Interdisciplinar , Lisboa 1649-003 , - - 2014
The accumulation of somatic mutations, to which the cellular genome is permanently exposed, often leads to cancer. Analysis of any tumour shows that, besides the malignant cells, one finds other 'supporting' cells such as fibroblasts, immune cells of various types and even blood vessels. Together, these cells generate the microenvironment ...
Felser Andrea A Clinical Pharmacology & Toxicology, University Hospital Basel, Switzerland; Department of Biomedicine, University of Basel, - - 2014
Benzbromarone is uricosuric, structurally related to amiodarone and a known mitochondrial toxicant. The aim of the current study was to improve our understanding in the molecular mechanisms of benzbromarone-associated hepatic mitochondrial toxicity. In HepG2 cells and primary human hepatocytes, ATP levels started to decrease in the presence of 25-50μM benzbromarone ...
Ahlberg Sebastian S Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, Berlin, - - 2014
Bacterial infections decreased considerably after the discovery of antibiotics. Nevertheless, because of the rising rate of infections caused by antibiotic-resistant bacteria strains, the search for new bactericidal agents has again become a crucial topic in clinical medicine. Silver nanoparticles (AgNP) have a huge potential in dermatology and wound care management ...
Medan Violeta V Dept. de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, Buenos Aires 1428, Argentina; Instituto de Fisiología, Biología Molecular y Neurociencias, CONICET, Argentina. Electronic address: - - 2014
The Mauthner-cell (M-cell) system of teleost fish has a long history as an experimental model for addressing a wide range of neurobiological questions. Principles derived from studies on this system have contributed significantly to our understanding at multiple levels, from mechanisms of synaptic transmission and synaptic plasticity to the concepts ...
Shen Baixin B Department of Urology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, - - 2014
To investigate the role and therapeutic potential of Nuclear factor erythroid-related factor 2 (Nrf2) in oxidative stress induced by di-N-butylphthalate (DBP) in testicular Leydig cells. Levels of reactive oxygen species (ROS) and Nrf2 in testicles from offspring of mice fed with DBP were studied. Basal ROS and Nrf2 level in ...
Wijdeven Ruud H RH Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The - - 2014
Chemical biology is a young and rapidly developing scientific field. In this field, chemistry is inspired by biology to create various tools to monitor and modulate biochemical and cell biological processes. Chemical contributions such as small-molecule inhibitors and activity-based probes (ABPs) can provide new and unique insights into previously unexplored ...
Ishitsuka Asako A Department of Dermatology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, - - 2014
Sphingosine kinase (SK), a key enzyme in sphingosine-1-phosphate (S1P) synthesis, is known to be overexpressed in various types of cancer cells. The effects of anticancer agents on SK1/S1P signaling have not yet been fully assessed in melanoma cells. In the present study, we investigated the effects of the combination of ...
Bu Qiang Q Department of Urology, Danyang People's Hospital, Zhenjiang, Jiangsu, P.R. - - 2014
Alterations in microRNA (miRNA) expression have been shown to be involved in the tumor response to chemotherapy. However, the possible role of miR‑101 in cisplatin sensitivity in human bladder cancer cells remains unclear. In this study, quantitative polymerase chain reaction and western blotting were utilized to determine the expression profiles ...
Milowska Katarzyna K Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, - - 2014
We have investigated whether polyamidoamine (PAMAM), phosphorus (pd) and viologen-phosphorus (vpd) dendrimers can prevent damage to embryonic mouse hippocampal cells (mHippoE-18) caused by rotenone, which is used as a pesticide, insecticide, and as a nonselective piscicide, that works by interfering with the electron transport chain in mitochondria. Several basic aspects, ...
Xie Chengyao C Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, China; Institute of Pathology and Pathophysiology, China Medical University, Shenyang, - - 2014
The aim of this study was to investigate c-Myc and β-catenin-mediated drug resistance in A549/DDP lung adenocarcinoma cells. Cisplatin sensitivity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) toxicity assay. β-Catenin and c-Myc protein expression following cisplatin treatment were determined using western blotting and immunofluorescence. Flow cytometry was performed to detect ...
Priyaja P P National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, 682016, - - 2014
Pyocyanin is a redox active phenazine pigment produced by Pseudomonas aeruginosa, with broad antibiotic activity having pharmacological, aquaculture, agriculture and industrial applications. In the present work cytotoxicity induced by pyocyanin is demonstrated in a human embryonic lung epithelial cell line (L-132), a rainbow trout gonad cell line (RTG-2) and a ...
Peiris Hassendrini N HN The University of Queensland Centre for Clinical Research, Brisbane, - - 2014
Context: Myostatin is a highly conserved secretory protein that negatively regulates muscle development by affecting both proliferation and differentiation of muscle cells. In human placentae the expression of myostatin is negatively correlated with gestational age and in placental explants, myostatin acts to facilitate glucose uptake. Myostatin expression is known to ...
D'Amato C C Cattedra di Oncologia Medica, Dipartimento di Medicina Clinica e Chirurgia, Università di Napoli "Federico II", Via S. Pansini 5, Napoli 80131, - - 2014
Background:Multiple lines of evidence support that the Hedgehog (Hh) signalling has a role in the maintenance and progression of different human cancers. Therefore, inhibition of the Hh pathway represents a valid anticancer therapeutic approach for renal cell carcinoma (RCC) patients. NVP-LDE225 is a Smoothened (Smo) antagonist that induces dose-related inhibition ...
Han S S Department of Neurosurgery, The First Hospital of China Medical University, Nanjing Street 155, Heping District, Shenyang 110001, - - 2014
Background:Insulin-like growth factor binding protein-2 (IGFBP-2) is significantly increased in the serum of patients with malignant gliomas. High plasma IGFBP-2 levels are correlated with poor prognosis in glioma patients. However, the exact role of exogenous IGFBP-2 in gliomas is unclear.Methods and results:Using the MTT cell viability assay, cell cycle analysis, ...
Pal Anoop K - - 2014
In vitro toxicity assessment of engineered nanomaterials (ENM), the most common testing platform for ENM, requires prior ENM dispersion, stabilization, and characterization in cell culture media. Dispersion inefficiencies and active aggregation of particles often result in polydisperse and multimodal particle size distributions. Accurate characterization of important properties of such polydisperse ...
Benitez Rafael R Department of Mathematics, University Center of Plasencia, University of Extremadura, Avda. Virgen del Puerto 2, 10600, Plasencia, - - 2014
This review reports on the use of the atomic force microscopy in the investigation of the mechanical properties of cells. It is shown that the technique is able to deliver information about the cell surface properties (e.g., topography), the Young modulus, the viscosity, and the cell the relaxation times. Another ...
Kroesen Bart-Jan BJ Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The - - 2014
MicroRNAs (miRNAs) are instrumental to many aspects of immunity, including various levels of T-cell immunity. Over the last decade, crucial immune functions were shown to be regulated by specific miRNAs. These "immuno-miRs" regulate generic cell biological processes in T-cells, such as proliferation and apoptosis, as well as a number of ...
Do Kwangseok K Department of Advanced Materials Chemistry, Korea University Sejong Campus, Sejong-ro 2511, Sejong City 339-700, Republic of Korea. - - 2014
Novel star-shaped hole transporting materials with a triazine unit have been synthesized. When the new was used as a hole transporting material in perovskite solar cells, the power conversion efficiency reached 12.51% under AM 1.5 G (100 mW cm(-2)) illumination, showing competitive photovoltaic performance with the widely used based solar ...
Oda Yukako - - 2014
When the surface view of each epithelial cell is compared with a polygon, its sides correspond to cell-cell junctions, while its vertices correspond to tricellular contacts, whose roles in epithelial cell morphogenesis have not been well studied. Here, we show that tricellulin, which is localized at tricellular contacts, regulates F-actin ...
Rolla Simona S Center for Experimental Research and Medical Studies, Azienda Ospedaliera Città della Salute e della Scienza di Torino, Turin, Italy; Department of Molecular Biotechnology and Health Sciences, Turin, - - 2014
Th1 and Th17 cells have been considered as effectors in mouse EAE and in the human counterpart, MS. Recently, IL-22, a Th17-related, proinflammatory cytokine, has been associated with a new Th cell subset, defined as Th22, involved in chronic inflammatory conditions, such as psoriasis; the role of IL-22 in MS ...
Xu Shuangbing S Huazhong University of Science and Technology, - - 2014
Cyclin-dependent kinases (CDKs) are the catalytic subunits of a family of mammalian heterodimeric serine/threonine kinases that play critical roles in the control of cell-cycle progression, transcription, and neuronal functions. However, the functions, substrates, and regulation of many CDKs are poorly understood. To systematically investigate these features of CDKs, we conducted ...
Maruthamuthu Venkat V Institute for Biophysical Dynamics, University of Chicago, Chicago, - - 2014
Knowing how epithelial cells regulate cell-matrix and cell-cell adhesions is essential to understand key events in morphogenesis as well as pathological events such as metastasis. During epithelial cell scattering, epithelial cell islands rupture their cell-cell contacts and migrate away as single cells on the extracellular matrix (ECM) within hours of ...
Fukumoto Motoi M Department of Pathology, Institute of Development, Aging and - - 2014
Standard fractionated radiotherapy for the treatment of cancer consists of daily irradiation of 2-Gy X-rays, 5 days a week for 5 to 8 weeks. To understand the characteristics of radioresistant cancer cells and to develop more effective radiotherapy, we established a series of novel, clinically relevant radioresistant (CRR) cells, which ...
Lee Sang-Soon SS From the *Department of Plastic and Reconstructive Surgery, Korea University Medical Center; and †Korea Electronics Technology - - 2014
The use of smartphones is expanding rapidly around the world, thus raising the concern of possible harmful effects of radiofrequency generated by smartphones. We hypothesized that Wi-Fi signals from smartphones may have harmful influence on adipose-derived stem cells (ASCs). An in vitro study was performed to assess the influence of ...
Choi Hyeju H Department of Advanced Material Chemistry, Korea University, 2511, Sejong-ro, Sejong City 339-700 (Republic of Korea), Fax: - - 2014
A new type of hole transporting material (HTMs) with an incorporated planar amine or triphenylamine as a core unit have been prepared. The two amine derivatives were demonstrated to be efficient hole transporting materials in fabricating solid-state organic-inorganic hybrid solar cells. Perovskite-based solar cells with a planar amine derivative gave ...
Wu Jingfang J aDepartment of Otorhinolaryngology bResearch Center, Affiliated Eye and ENT Hospital of Fudan University cInstitute of Stem Cell and Regeneration Medicine, Institutions of Biomedical Science dState Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, - - 2014
The ability of nonmammalian vertebrates to regenerate hair cells (HCs) after damage-induced HC loss has stimulated and inspired research in the field of HC regeneration. The protein pRb encoded by retinoblastoma gene Rb1 forces sensory progenitor cells to exit cell cycle and maintain differentiated HCs and supporting cells (SCs) in ...
O'Callaghan Carol C Departments of aPharmacology and Therapeutics bMedicine, University College Cork, Cork, - - 2014
We recently documented p38δ differential expression and function in oesophageal squamous cell carcinoma (OESCC). This study expands upon these findings and investigates whether p38δ status in OESCC can influence response(s) to cytotoxic drugs. The antiproliferative effect of conventional cisplatin and 5-fluorouracil (CF) treatment was compared with the recently reviewed triple ...
Temizel Emine E Department of Chemistry, Faculty of Arts and Science, Fatih University, Istanbul, 34500, - - 2014
Porphyrin photosensitizers are mostly used components in Photodynamic Therapy (PDT). The poor solubility of porphyrins in aqueous medium is the problem to be solved for the in vivo applications. The delivery of photosensitizers to the tumor cells using liposome vehicles can help to overcome this problem. In this work, we ...
Michels Judith J Equipe 11 labellisée par la Ligue Nationale contre le Cancer, Centre de Recherche des Cordeliers, INSERM U1138, F-75006 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, F-75005 Paris, France; Metabolomics and Cell Biology Platforms, Gustave Roussy, F-94805 Villejuif, France; Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, F-75015 Paris, - - 2014
The selection of human cancer cell lines in cis-diamminedichloroplatinum(II) (CDDP, best known as cisplatin) is accompanied by stereotyped alterations that contribute to the acquisition of a CDDP-resistant state. Thus, CDDP resistance often leads to the upregulation of the DNA repair enzyme poly (ADP-ribose) polymerase-1 (PARP1) with the consequent intracellular accumulation ...
Cosker François F From the Biomedical Institute of the Brazilian Semiarid (INCT-IBISAB-CNPq), Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil 60430-270. Electronic address: - - 2014
The nitroderivative 1-nitro-2-phenylethane (NPE) was recently described as a compound possessing heme-dependent soluble guanylyl cyclase (sGC) stimulating properties in vascular smooth muscle cells. In this study, we tested such pharmacological property of NPE in mice pancreatic acinar cells subjected to the bile salt taurocholate, a type of pathological stimulus that ...
Liu Wei-Ting WT Department of Pharmacology, College of Medicine, National Cheng-Kung University, Tainan, - - 2014
Malignant gliomas are among the most devastating cancers as they are resistant to many kinds of treatment. Despite recent advances in the diagnosis and treatment, the prognosis of patients remains very poor and the development of new drug is urgently needed. Here, we report that a synthetic quinazolinone analog 2-(naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone ...
Floen Miranda J MJ Basic Biomedical Sciences, The University of South Dakota Sanford School of Medicine, Sioux Falls, SD - - 2014
The most common form of newborn chronic lung disease, bronchopulmonary dysplasia (BPD), is thought to be caused by oxidative disruption of lung morphogenesis which results in decreased pulmonary vasculature and alveolar simplification. Although cellular redox status is known to regulate cellular proliferation and differentiation, redox-sensitive pathways associated with these processes ...
Jobin Marie-Lise ML CBMN-Univ Bordeaux, UMR 5248, Allée Geoffroy St Hilaire, 33600 Pessac, France. Electronic address: - - 2014
Cell-penetrating peptides (CPPs) are small molecules of major interest due to their ability to efficiently transport large cargos across cell membranes in a receptor- and energy-independent way and without being cytotoxic to cells. Since their discovery 20 years ago their potential interest in drug delivery and therapeutic diagnosis became undeniable. ...
Liu Ting T Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, - - 2014
Inflammasome-mediated caspase-1 activation is involved in cell death and the secretion of the proinflammatory cytokine interleukin-1β (IL-1β). Although the dynamics of caspase-1 activation, IL-1β secretion, and cell death have been examined with bulk assays in population-level studies, they remain poorly understood at the single-cell level. In this study, we conducted single-cell ...
Cencetti Francesca F Dipartimento di Scienze Biomediche Sperimentali e Cliniche "Mario Serio", Università di Firenze, Viale G,B. Morgagni 50, 50134, Firenze, ITALY; Istituto Interuniversitario di - - 2014
Regulation of motility of skeletal muscle precursor cells, such as satellite cells, is critically important for their proper recruitment at the site of tissue damage and ultimately for its correct repair. Here we show that lysophosphatidic acid (LPA), well-recognized as powerful bioactive agent, strongly stimulates cell migration of activated murine ...
Dana Dibyendu D Department of Chemistry and Biochemistry, Queens College and the Graduate Center of the City University of New York, Queens, New York 11367-1597, USA. - - 2014
A hybrid-design approach is undertaken to develop a highly potent and selective inhibitor of human cathepsin L. Studies involving human breast carcinoma MDA-MB-231 cells establish that this inhibitor can successfully block intracellular cathepsin L activity, and retard the cell-migratory potential of these highly metastatic cells.
Deng Xiao-Mei - - 2014
Plasma cells, which secrete auto-antibodies, are considered to be the arch-criminal of autoimmune diseases such as systemic lupus erythematosus, but there are many cytokines involved in inducing the differentiation of B-cell subsets into plasma cells. Here, we emphasize IL-21, which has emerged as the most potent inducer of plasma cell ...
Vassilopoulos A A 1] Genetics of Development and Disease Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA [2] Department of Radiation Oncology, Feinberg School of Medicine, Northwestern University, Chicago, IL, - - 2014
The tyrosine kinase WEE1 controls the timing of entry into mitosis in eukaryotes and its genetic deletion leads to pre-implantation lethality in mice. Here, we show that besides the premature mitotic entry phenotype, Wee1 mutant murine cells fail to complete mitosis properly and exhibit several additional defects that contribute to ...
Ogasawara Sachiko S Department of Pathology, Kurume University School of - - 2014
Human combined hepatocellular-cholangiocarcinoma (CHC) expresses several hepatic stem/progenitor cell (HSPC) markers, suggesting this neoplasm originates from HSPCs. We examined the significance of HSPC marker in CHC using a human CHC cell line. We used a human CHC cell line (KMCH-1) previously established in our laboratory. The original tumor was classified ...
Ninagawa Satoshi S Department of Biophysics, Graduate School of Science, and Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606-8502, Japan Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, - - 2014
Glycoproteins misfolded in the endoplasmic reticulum (ER) are subjected to ER-associated glycoprotein degradation (gpERAD) in which Htm1-mediated mannose trimming from the oligosaccharide Man8GlcNAc2 to Man7GlcNAc2 is the rate-limiting step in yeast. In contrast, the roles of the three Htm1 homologues (EDEM1/2/3) in mammalian gpERAD have remained elusive, with a key ...
Akiyoshi Sayaka S Graduate School of Systems Life Sciences, Kyushu University, Fukuoka 812-8581, - - 2014
In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the C. elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and ...
Saavedra-Ávila Noemí Alejandra NA http://orcid.org/0000-0003-3064-7254 Immunology Unit, Department of Experimental Medicine, Faculty of Medicine, University of Lleida, 25008 Lleida, Spain;Institut de Recerca Biomèdica de Lleida, 25198 Lleida, - - 2014
Type 1 diabetes is an autoimmune condition caused by the lymphocyte-mediated destruction of the insulin-producing β cells in pancreatic islets. We aimed to identify final molecular entities targeted by the autoimmune assault on pancreatic β cells that are causally related to β cell viability. Here, we show that cyclin D3 ...
Yang Zhang Z University of Copenhagen, - - 2014
The Chinese hamster ovary cell (CHO) is the major host cell factory for recombinant production of biological therapeutics primarily due to its "human-like" glycosylation features. CHO is used for production of several O-glycoprotein therapeutics including erythropoietin, coagulation factors, and chimeric receptor IgG1-Fc-fusion proteins, however, some O-glycoproteins are not produced efficiently ...
Nowotarski Stephanie H SH Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, - - 2014
Cells have evolved an elegant tuning mechanism to maintain tissue integrity, in which increasing mechanical tension stimulates actin assembly at cell-cell junctions. The mechanosensitive junctional protein α-catenin acts through vinculin and Ena/VASP proteins to reinforce the cell against mechanical stress.
Xu Huiming H Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, - - 2014
Tremendous efforts have been made to elucidate the molecular mechanisms that control the specification of definitive endoderm cell fate in gene-knockout mouse models and ESCs differentiation models, however the impact of unfolded protein response (UPR) due to the stress of the endoplasmic reticulum (ER) on the endodermal specification is not ...
Wang Wen-Horng WH Department of Medicinal Chemistry and Molecular - - 2014
The Syk protein-tyrosine kinase, a well characterized regulator of immune cell function, plays an increasingly recognized role in tumorigenesis as a promoter of cell survival in both hematological and nonhematological malignancies. We show here that the expression of Syk in MCF7 or MDA-MB-231 breast cancer cells or in DG75 B ...
Cronin Therese T Center for Advanced Retinal and Ophthalmic Therapeutics, F.M. Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA, USA Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland jebennet@mail.med.upenn.edu - - 2014
In this report, we describe the development of a modified adeno-associated virus (AAV) capsid and promoter for transduction of retinal ON-bipolar cells. The bipolar cells, which are post-synaptic to the photoreceptors, are important retinal targets for both basic and preclinical research. In particular, a therapeutic strategy under investigation for advanced ...
Wu Chia-Yen CY Department of Physiology, Tzu Chi University, No. 701, Sec. 3, Chung Yang Rd., Hualien, 97004, - - 2014
Hair cells are highly sensitive to environmental insults and other therapeutic drugs. The adverse effects of drugs such as aminoglycosides can cause hair cell death and lead to hearing loss and imbalance. The objective of the present study was to evaluate the protective activity of L-ascorbic acid, N-acetylcysteine (NAC) and ...
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