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Results 301 - 350 of 29048
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Crowell Elizabeth Faris - - 2014
The midbody remnant (MBR) that is generated after cytokinesis abscission has recently attracted a lot of attention, since it may have crucial consequences for cell differentiation and tumorigenesis in mammalian cells. In those cells, it has been reported that the MBR is either released into the extracellular medium, or retracted ...
Fendyur Anna A Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Room 36-824, Cambridge, Massachusetts 02139, United - - 2014
Understanding how newly engineered micro- and nanoscale materials and systems that interact with cells impact cell physiology is crucial for the development and ultimate adoption of such technologies. Reports regarding the genotoxic impact of forces applied to cells in such systems that can both directly or indirectly damage DNA emphasize ...
Kang Pil Jung PJ Department of Molecular Genetics and Molecular Cellular Developmental Biology Program, The Ohio State University, Columbus, OH - - 2014
Cell polarization occurs along a single axis that is generally determined by a spatial cue, yet the underlying mechanism is poorly understood. Using biochemical assays and live-cell imaging, we show that cell polarization to a proper growth site requires activation of Cdc42 by Bud3 in haploid budding yeast. Bud3 catalyzes ...
Kumaresan Pappanaicken R PR Division of - - 2014
Clinical-grade T cells are genetically modified ex vivo to express chimeric antigen receptors (CARs) to redirect their specificity to target tumor-associated antigens in vivo. We now have developed this molecular strategy to render cytotoxic T cells specific for fungi. We adapted the pattern-recognition receptor Dectin-1 to activate T cells via ...
Jung Hye-Won HW Department of Life Science, Kyonggi University, Suwon, 443-760, Republic of - - 2014
Cannabinoids display various pharmacological activities, including tumor regression, anti-inflammatory and neuroprotective effects. To investigate the molecular mechanisms underlying the pharmacological effects of cannabinoids, we used a yeast two-hybrid system to screen a mouse brain cDNA library for proteins interacting with type 1 cannabinoid receptor (CB1R). Using the intracellular loop 3 ...
Patiño Sandra Fernanda Suárez SF Laboratório de Imunologia Viral, Instituto Butantan, São Paulo, Brazil; Departamento de Engenharia Química, Universidade Federal de São Carlos, São Carlos, - - 2014
The transient transfection process has been developed to allow rapid production of recombinant proteins. In this paper, we describe the transient expression of recombinant rabies virus glycoprotein (RVGP) in Drosophila melanogaster Schneider 2 (S2) cells. Different cell transfection reagents were evaluated, together with the effects of different cell cultivation procedures ...
Acarregui Argia A NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, - - 2014
Since the introduction of the cell immunoisolation as an alternative to protect transplanted cells from host immune attack much effort has been made to lead this technology into a realistic clinical proposal. Several promising approaches have been investigated to resolve the biotechnological and biosafety challenges related with cell microencapsulation. Here, ...
Hegmans Joost Pjj JP Erasmus MC Cancer Institute, Department of Pulmonary Medicine, Ee 2253a, Dr. Molewaterplein 50, 3045 PE Rotterdam, The Netherlands. Electronic address: - - 2014
We have to strengthen our 'chess-playing skills' when using immunotherapeutic approaches in cancer treatment: know the cancerous opponent, study its evolution and have an eye for its weaknesses. Besides tumor cells, other pieces on the board are stromal cells, endothelial cells and different immune cells. Some of these immune cells, ...
Rolando Monica M Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France; Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche (UMR) 3525, Paris, - - 2014
Intracellular bacterial pathogens modulate the host response to persist and replicate inside a eukaryotic cell and cause disease. Legionella pneumophila, the causative agent of Legionnaires' disease, is present in freshwater environments and represents one of these pathogens. During coevolution with protozoan cells, L. pneumophila has acquired highly sophisticated and diverse ...
Ma Wei-Feng WF Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, PR - - 2014
A series of novel 2,4-diaminopyrimidines containing piperidine and piperazine moieties were synthesized via an efficient one-pot methodology. The bioassay tests demonstrated that compounds 27 and 28 displayed much stronger antitumor activities against four human cancer cell lines (HepG2, A549, MDA-MB-231 and MCF-7) than positive control fluorouracil. Particularly, compound 28 showed ...
Wang Rui R Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, China; Co-first - - 2014
Chemoresistant tumors usually fail to respond to radiotherapy. However, the mechanisms involved in chemo- and radiotherapy cross resistance are not fully understood. Previously, we have identified microRNA (miR)-451 as a tumor suppressor in lung adenocarcinoma (LAD). However, whether miR-451 plays critical roles in chemo- and radiotherapy cross resistance in LAD ...
Han Tao T Department of Orthopedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, P.R. - - 2014
The aim of the present study was to establish a new cisplatin-resistant human osteosarcoma cell line and investigate its biological characteristics. The human osteosarcoma cell line SOSP-9607 was exposed to cisplatin by stepwisely increasing the concentrations in the medium to select for the drug-resistant subline, SOSP-9607/CDDP cells. The morphological features ...
Hu Bing B Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 202032, P.R. - - 2014
Nü-zhen-zi, the fruit of Ligustrum lucidum Ait., is one of the most frequently used liver Yin tonifying Chinese herbs for the treatment of liver cancer. However, the effect of Ligustrum lucidum fruit on hepatocarcinoma cells remains unknown. In the present study, we evaluated the effects of a Ligustrum lucidum fruit extract (LLFE) on ...
Jeon Nam Joong NJ 1] Division of Advanced Materials, Korea Research Institute of Chemical Technology, 141 Gajeong-Ro, Yuseong-Gu, Daejeon 305-600, Korea - - 2014
Organolead trihalide perovskite materials have been successfully used as light absorbers in efficient photovoltaic cells. Two different cell structures, based on mesoscopic metal oxides and planar heterojunctions have already demonstrated very impressive advances in performance. Here, we report a bilayer architecture comprising the key features of mesoscopic and planar structures ...
Ozerkan Dilşad D Department of Biology, Faculty of Arts and Sciences, Kastamonu University, 37100, Kastamonu, Turkey, - - 2014
Carbon tetrachloride (CCl4) is widely used to induce liver toxicity in in vitro/in vivo models. Lipid peroxidation (LPO) begins with toxicity and affects cell viability. Recently, the beneficial effects of melatonin and Vitamin D on cell proliferation in human normal and cancer cells were found. This study was planned to ...
Torii Koji K Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, - - 2014
Treatment of peritoneal carcinomatosis arising from gastric cancer remains a considerable challenge. In recent years, the anticancer effect of nonequilibrium atmospheric pressure plasma (NEAPP) has been reported in several cancer cell lines. Use of NEAPP may develop into a new class of anticancer therapy that augments surgery, chemotherapy, and radiotherapy. ...
Ash S A SA School of Medicine and Medical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland Department of Anaesthesia, Mater Misericordiae University Hospital, Eccles Street, Dublin 7, - - 2014
While volatile agents have been implicated in metastasis-enhancing effects on cancer cells, the effects of xenon are unknown. We investigated xenon- and sevoflurane-mediated effects on migration and expression of angiogenesis biomarkers in human breast adenocarcinoma cells. MDA-MB-231 and MCF-7 cells were exposed to xenon 70% with O2 25%, CO2 5%; ...
Yan Guoxin G Department of Stomatology, Wuxi No.2 People's Hospital, Wuxi 214002, - - 2014
Ras homolog gene family member A (RhoA) has been identified as a critical regulator of tumor aggressive behavior. In this study, we assessed the role of RhoA in the mechanisms underlying growth, migration, and invasion of squamous cell carcinoma of tongue (TSCC). Stable RhoA knockdown of TSCC cell lines SCC-4 ...
Park Seung Bin SB Department of Obstetrics and Gynecology, Dong-A University, College of Medicine, Busan, - - 2014
Our aim was to investigate whether celecoxib, a cyclooxygenase 2 (COX-2) inhibitor, decreases the in vitro proliferation of leiomyoma cells if the inflammatory pathway is blocked. Menstruation is an inflammation of uterus that produces cytokines and prostanoids, but the inflammatory mechanism underlying the growth of leiomyoma remains unexplained. Using in ...
Kang Woo Kyu WK Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 305-764, Republic of - - 2014
Silent Information Regulator 2 (Sir2), a conserved NAD(+)-dependent histone deacetylase, has been implicated as one of the key factors in regulating stress response and longevity. Here, we report that the role of Sir2 in oxidative stress resistance and chronological lifespan is dependent on growth phase in yeast. In exponential phase, ...
Liao Yun - - 2014
Mechanisms governing the metastasis of endometrial cancer (EC) are poorly defined. Recent data support a role for Enhancer-of-split and hairy-related protein 1 (SHARP1), a basic helix-loop-helix transcription repressor, in regulating invasiveness and angiogenesis of several human cancers. However, the role of SHARP1 in metastasis of EC remains unclear. Human EC ...
Dickinson Sally E SE Arizona Cancer Center, University of Arizona, Tucson, AZ; Department of Pharmacology, University of Arizona, Tucson, AZ. Electronic address: - - 2014
One of the primary components of the East Indian sandalwood oil (EISO) is α-santalol, a molecule that has been investigated for its potential use as a chemopreventive agent in skin cancer. Although there is some evidence that α-santalol could be an effective chemopreventive agent, to date, purified EISO has not ...
Li Wen W Department of Biological and Chemical Sciences, Illinois Institute of Technology, 3101S. Dearborn ST., Chicago, IL 60616, United - - 2014
It has been well established that an aligned matrix provides structural and signaling cues to guide cell polarization and cell fate decision. However, the modulation role of cells in matrix remodeling and the feedforward effect on stem cell differentiation have not been studied extensively. In this study, we report on ...
Chi Wenying W Department of Anesthesiology, Weifang Medical University, Weifang City 261053, Shangdong Province, PR - - 2014
MicroRNAs (miRNAs) have emerged as a major regulator in neurological diseases, and understanding their molecular mechanism in modulating cerebral ischemic injury may provide potential therapeutic targets for ischemic stroke. However, as one of 19 differentially expressed miRNAs in mouse brain with middle cerebral artery occlusion (MCAO), the role of miR-134 ...
Li Guicai G Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 226001 Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR - - 2014
Chitosan is found to promote the regeneration of peripheral nerve system in our previous studies, whereas the regeneration speed is not satisfied with clinical request. Micropatterning could promote cell orientation and growth, however, the effect of porous chitosan micropatterning on nerve regeneration is rarely reported. In this study, the porous ...
Ram Babul Moni BM Centre for DNA Fingerprinting and Diagnostics, Laboratory Block, Tuljaguda complex, Nampally, Hyderabad, Andhra Pradesh, 500001, India. Electronic address: - - 2014
Cyclosporine A (CsA), a widely used immunosuppressant shows cytotoxic effects by either inducing apoptosis or redirecting the cell towards non apoptotic cell death. However, there still remains a lacuna in understanding the mechanism of CsA induced non apoptotic cell death. In the present study we investigated calcineurin dependent or independent ...
Butler Michael M Department of Microbiology, University of Manitoba, Winnipeg, Manitoba R3T2N2, Canada. Electronic address: - - 2014
Non-human mammalian cells such as CHO have been used predominantly for the production of biopharmaceuticals including monoclonal antibodies (Mabs). Although the glycosylation profile of these products is 'human-like' there is still the possibility of immunogenic epitopes such as α-Gal and Neu5Gc. Human cell lines have now been designed for high ...
Kegler K K Department of Pathology, University of Veterinary Medicine, Bünteweg 17, D-30559 Hannover, - - 2014
Congenital teratomas arising in the coccygeal region have not been reported in domestic animals. This report describes a congenital coccygeal teratoma in a male Burmese cat. A round to oval expansile mass with solid and cystic areas was found ventral to the 5th to 7th caudal vertebrae. Microscopically, the tumour ...
Zhu Yuhua Y State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, - - 2014
L-Glutamine (Gln) is an essential amino acid for intestinal growth and integrity. However, the underlying molecular mechanisms are not fully known. In the present study, porcine intestinal epithelial cells (IPEC-1) were used to test the hypothesis that autophagy is induced by Gln deprivation and inhibited by Gln supplementation. After a ...
Pan Banzhou - - 2014
Docetaxel resistance remains a major obstacle in the treatment of non-small cell lung cancer (NSCLC). High-mobility group box 1 (HMGB1) has been shown to promote autophagy protection in response to antitumor therapy, but the exact molecular mechanism underlying HMGB1-mediated autophagy has not been clearly defined. Lung adenocarcinoma (LAD) cells were ...
Pietsch Constanze C Institute of Natural Resource Sciences (IUNR), Zurich University of Applied Sciences (ZHAW), Gruental, P.O. Box, 8820, Waedenswil, Switzerland, - - 2014
The worldwide distribution and high bioaccumulation potential of pentachlorophenol (PCP) in aquatic organisms imply a high toxicological impact in aquatic systems. Firstly, our investigations show that, similar to mammalian cell lines, PCP can be metabolized to tetrachlorohydroquinone (TCHQ) in the permanent cell line derived from rainbow trout liver cells (RTL-W1). ...
Liu Nianli N Department of Molecular Medicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China; Research Center of Cancer Prevention & Treatment, Translational Medicine Research Center of Liver Cancer, Hunan Provincial Tumor Hospital (Affiliated Tumor Hospital of Xiangya Medical School of Central South University), Changsha 410013, - - 2014
Newcastle disease virus (NDV) oncolysis is believed to be facilitated by a defective Type I interferon (IFN) response. We compared hepatocellular carcinoma (HCC)-derived cell lines and found that TRAIL-resistant cells were more susceptible to NDV oncolysis than were TRAIL-sensitive cells. In examining the IFN response, we found that basal expression ...
Bear James E JE UNC-Chapel Hill, UNC Lineberger Cancer Center and the Department of Cell Biology and Physiology, United States; Howard Hughes Medical Institute, UNC-Chapel Hill, United States. Electronic address: - - 2014
Cell migration directed by spatial cues, or taxis, is a primary mechanism for orchestrating concerted and collective cell movements during development, wound repair, and immune responses. Compared with the classic example of amoeboid chemotaxis, in which fast-moving cells such as neutrophils are directed by gradients of soluble factors, directed migration ...
Feng Guangxue G Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117585, Singapore; Environmental Research Institute, National University of Singapore, Singapore 117411, - - 2014
Noninvasive fluorescence cell tracking provides critical information on the physiological displacement and translocation of actively migrating cells, which deepens our understanding of biomedical engineering, oncological research, stem cell transplantation and therapies. Non-viral fluorescent protein transfection based cell tracing has been widely used but with issues related to cell type-dependent expression, ...
Nicolella Heloiza Diniz HD Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600, Franca, São Paulo, - - 2014
Salvia officinalis (sage) is a perennial woody subshrub native to the Mediterranean region that is commonly used as a condiment and as an anti-inflammatory, antioxidant and antimicrobial agent due to its biological activities. Manool is the most abundant micro-metabolite found in Salvia officinalis essential oils and extracts. We therefore decided ...
Fedorchak Gregory R GR Department of Biomedical Engineering & Weill Institute for Cell and Molecular Biology; Cornell University, Ithaca, - - 2014
Cells respond to mechanical forces by activating specific genes and signaling pathways that allow the cells to adapt to their physical environment. Examples include muscle growth in response to exercise, bone remodeling based on their mechanical load, or endothelial cells aligning under fluid shear stress. While the involved downstream signaling ...
Lipiec Ewelina E The Henryk Niewodniczanski Institute of Nuclear Physics, PAN, 31-342 Kraków, Poland. - - 2014
SR-FTIR in combination with Principal Component Analysis (PCA) was applied to investigate macromolecular changes in a population of melanocytes and their extracted nuclei induced by environmentally relevant fluxes of UVR (Ultraviolet Radiation). Living cells and isolated cellular nuclei were investigated post-irradiation for three different irradiation dosages (130, 1505, 15 052 Jm(-2) ...
Balsam Joshua J Division of Biology, Office of Science and Engineering, FDA, Silver Spring, MD 20993, - - 2014
Here we describe a novel low-cost flow cytometer based on a webcam capable of low cell number detection in a large volume which may overcome the limitations of current flow cytometry. Several key elements have been combined to yield both high throughput and high sensitivity. The first element is a ...
Takahashi Ryutaro R Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, - - 2014
One of hallmarks of cancer, global gene expression alteration is closely associated with the development and malignant characteristics associated with adult T-cell leukemia (ATL) as well as other cancers. Here we show that aberrant overexpression of Ellis Van Creveld (EVC) family is responsible for cellular Hedgehog (HH) activation, which provides ...
Müller Werner E G WE ERC Advanced Investigator Grant Research Group at Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, D-55128 Mainz, GERMANY. - - 2014
Bioprinting/3D cell printing procedures for the preparation of scaffolds/implants have the potential to revolutionize regenerative medicine. Besides biocompatibility and biodegradability, the hardness of the scaffold material is of critical importance to allow sufficient mechanical protection and, to the same extent, allow migration, cell-cell and cell-substrate contact formation of the matrix-embedded ...
Hazane-Puch Florence F Unité de Biochimie Hormonale et Nutritionnelle, Département de Biochimie, Toxicologie et Pharmacologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire de Grenoble, CS 10217, 38043 Grenoble, France. - - 2014
Selenium (Se) is an essential trace element with a narrow safety zone and unclear effects on skin photoageing. The aim of this work was to investigate the photoageing properties of sodium selenite or selenomethionine (SeMet) after a long term (6 days) Se supplementation in normal human skin fibroblasts (NHSF) subjected ...
Dandekar Smita S NYU Langone Medical Center, NYU Cancer Institute, New York, NY, - - 2014
While childhood acute lymphoblastic leukaemia (ALL) is now highly curable, the dismal prognosis for children who relapse warrants novel therapeutic approaches. Previously, using an integrated genomic analysis of matched diagnosis-relapse paired samples, we identified overactivation of the Wnt pathway as a possible mechanism of recurrence. To validate these findings and ...
Kumar Sanjay S Biomolecular Science Centre, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Bld 20, 4110 Libra Drive, Orlando, FL, 32816, USA, - - 2014
Hypermethylated in cancer 1 (HIC1) is a novel tumor suppressor gene (tsg) frequently silenced by epigenetic modification, predominantly by methylation in different tumors. HIC1 functionally co-operates with p53 in cultured cells as well as in transgenic animals to suppress tumors and has binding site on its promoter. Its over expression ...
Mora-Bermúdez Felipe F Max Planck Institute of Molecular Cell Biology and Genetics , Dresden , - - 2014
Mitotic spindle orientation is crucial for symmetric vs. asymmetric cell division and depends on astral microtubules. Here, we show that distinct subpopulations of astral microtubules exist, which have differential functions in regulating spindle orientation and division symmetry. Specifically, in polarized stem cells of developing mouse neocortex, astral microtubules reaching the ...
Spivey Eric C - - 2014
Due to their short lifespan, rapid division and ease of genetic manipulation, yeasts are popular model organisms for studying aging in actively dividing cells. To study replicative aging over many cell divisions, individual cells must be continuously separated from their progeny via a laborious manual microdissection procedure. Microfluidics-based soft-lithography devices ...
Kanazawa Takeharu T Department of Otolaryngology‑Head and Neck Surgery, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329‑0498, - - 2014
Galanin and its receptors, GALR1 and GALR2, are tumor suppressors and represent therapeutic targets in head and neck squamous cell carcinoma (HNSCC). In the present study, it was demonstrated that the re‑expression of GALR1 in GALR1 and GALR2‑negative HNSCC cells suppresses tumor cell proliferation. This is mediated via extracellular‑regulated protein ...
Plosky Brian S BS Molecular Cell, Cell Press, 600 Technology Square, 5(th) Floor, Cambridge, MA 02139, USA. Electronic address: - - 2014
Very few specific functions have been assigned to ultraconserved regions. In this issue of Molecular Cell, Liz et al. (2014) describe how a lncRNA transcribed from an ultraconserved region can negatively regulate miRNA maturation.
Nilsson Bo B Dept. of Immunology, Genetics and Pathology (IGP), Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden. Electronic address: - - 2014
Cell therapies in which the cells come into direct contact with blood and other body fluids are emerging treatment procedures for patients with various diseases, such as diabetes mellitus, liver insufficiency, and graft-versus-host disease. However, despite recent progress, these procedures are associated with tissue loss caused by thromboinflammatory reactions. These ...
Deng Haijing H Pathology Department, Hebei Medical University, Shi Jiazhuang, - - 2014
The synthetic tetrapeptide N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) has been shown to be a modulator of molecular aspects of the fibrosis pathway. This study reveals that Ac-SDKP exerts an anti-fibrotic effect on human type II alveolar epithelial cells (A549), which are a source of myofibroblasts once exposed to TGF-β1, by decreasing the expression ...
Karam Jean-Pierre JP LUNAM Université, UMR S-1066F-49933 Angers, France; INSERM U1066, MINT "Micro et Nanomédecines Biomimétiques "F-49933 Angers, France; National Institute for Cardiovascular Research (INRC) I-40126 Bologna, Italy; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, 40126 Bologna, - - 2014
The challenge of tissue engineering of the infarcted heart is how to improve stem cell engraftment, survival, homing, and differentiation for myocardial repair. We here propose to integrate human adipose-derived stem cells (ADSCs) and pharmacologically active microcarriers (PAMs), a threedimensional (3D) carrier of cells and growth factors, into an injectable ...
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