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Brézillon Stéphane S Laboratoire de Biochimie médicale et de Biologie Moléculaire, UFR de Médecine, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095, Reims Cedex, - - 2014
We recently identified vibrational spectroscopic markers characteristic of standard glycosaminoglycan (GAG) molecules. The aims of the present work were to further this investigation to more complex biological systems and to characterize, via their spectral profiles, cell types with different capacities for GAG synthesis. After recording spectral information from individual GAG ...
La Regina Giuseppe - - 2014
We synthesized 3-aroyl-1-arylpyrrole (ARAP) derivatives as potential anticancer agents having different substituents at the pendant 1-phenyl ring. Both the 1-phenyl ring and 3-(3,4,5-trimethoxyphenyl)carbonyl moieties were mandatory to achieve potent inhibition of tubulin polymerization, binding of colchicine to tubulin and cancer cell growth. ARAP 22 showed strong inhibition of the P-glycoprotein-overexpressing ...
Jin Hyeon-Ok HO KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-709, Republic of - - 2014
Piperlongumine (PL) has been shown to selectively induce apoptotic cell death in cancer cells via reactive oxygen species (ROS) accumulation. In this study, we characterized a molecular mechanism for PL-induced cell death. Cell viability and cell death were assessed by MTT assay and Annexin V-FITC/PI staining, respectively. ROS generation was ...
Li Yafeng Y State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou, Fujian 350002, China. - - 2014
Metal-organic frameworks (MOFs) have been used in the area of photovoltaic devices via a novel approach proposed here for the first time. Hierarchical ZnO parallelepipeds were prepared from a MOF precursor, which could then act as an effective light scattering layer in dye-sensitized solar cells, leading to significantly improved cell ...
Sun Xun X State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou, Fujian 350002, China. - - 2014
NiS nanosheets on ITO substrates were synthesized with in situ growth via a one-step hydrothermal route, and exhibited an efficiency of 7.08% which was comparable to the solar cell based on the Pt electrode (7.01%) under the similar conditions.
Heavey Susan S Thoracic Oncology Research Group; Institute of Molecular Medicine; Trinity Centre for Health Sciences; St. James's Hospital; Dublin, - - 2014
Chemoresistance is a major therapeutic challenge to overcome in NSCLC, in order to improve the current survival rates of<15% at 5 years. We and others have shown increased PI3K signaling in NSCLC to be associated with a more aggressive disease, and a poorer prognosis. In this study, targeted inhibition of ...
I┼čeri Ozlem Darcansoy OD Institute of Transplantation and Gene Sciences, Baskent University , Ankara , Turkey - - 2014
Abstract Context: Propranolol, atenolol, and ICI118,551 are non-selective β-adrenergic receptor (AR), β1-AR, and β2-AR antagonists, respectively. Objective: We investigated the efficacy of propranolol, atenolol, and ICI118,551 on proliferation, migration, and invasion of non-stimulated breast (MCF7), colon (HT-29), and hepatocellular (HepG2) cancer cells. Materials and methods: β-AR expression profiling of cells ...
Shokoohinia Yalda Y Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences , Kermanshah , Iran - - 2014
Abstract Context: Prangos ferulacea (L.) Lindl. (Apiaceae) is a perennial plant found in the Middle-East, where it is commonly used as an antispasmodic and anti-inflammatory agent. It is a rich source of coumarins. Objective: To purify several coumarins from P. ferulacea and to screen their cytotoxicity and anti-herpes activity. Materials ...
Hung Chien-Jen CJ Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan ; - - 2014
In this study, we investigated the fine regulation of cell-to-cell movement of Bamboo mosaic virus (BaMV). We report that the coat protein (CP) of BaMV is phosphorylated in planta at position serine 241 (S241), in a process involving Nicotiana benthamiana casein kinase 2α (NbCK2α). BaMV CP and NbCK2α colocalize at ...
Kaur Gurpreet G Department of Environment Studies, Panjab University, Chandigarh, 160014, - - 2014
Plant responses to abiotic stress significantly affect the development of cells, tissues and organs. However, no studies correlating Pb-induced mitotic inhibition and DNA damage and the alterations in redox homeostasis during root division per se were found in the literature. Therefore, an experiment was conducted to evaluate the impact of ...
Li Heping - - 2014
Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancer in developing countries. The prognosis and survival rate of ESCC are very poor. Recently, microRNAs (miRNAs) have emerged as important regulators of cancer cell biological processes. To better understanding the molecular mechanisms by which they regulate the ...
He Juan J College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China. - - 2014
A comprehensive elucidation of the unexpected adverse events that occur in skeletal myoblast transplantation is fundamental for the optimization of myocardial therapeutic effects. However, a well-defined method to study the interactions between skeletal myoblasts and cardiomyocytes during the healing process is out of reach. Here, we describe a microfluidic method ...
Kis M M Department of Medical Physics, University of Szeged, Rerrich Béla tér 1, Szeged, 6720, - - 2014
The development of photosynthetic membranes of intact cells of Rhodobacter sphaeroides was tracked by light-induced absorption spectroscopy and induction and relaxation of the bacteriochlorophyll fluorescence. Changes in membrane structure were induced by three methods: synchronization of cell growth, adjustment of different growth phases and transfer from aerobic to anaerobic conditions ...
Liu Yin - - 2014
Organically modified rectorite (OREC) miro/nanoparticles can be synthesized by organic modification from calcium rectorite (Ca2+-REC or REC), a common form of rectorite in nature. Although OREC has potential applications in food packing and drug delivery, its cytotoxicity is not clear. In the present study, we investigated and compared the cytotoxicity ...
Zheng Hui H Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, - - 2014
Accumulating evidence indicates that the mesenchymal-epithelial transition (MET) and epithelial-mesenchymal transition (EMT) are basic mechanisms for cell fate conversion and may help us understand both physiologic and pathologic processes such as development and carcinogenesis. Here, we further suggest that mammalian cells fall into two grand divisions, mesenchymal or epithelial; interconversions ...
Banfalvi Gaspar G Department of Microbial Biotechnology and Cell Biology, University of Debrecen, 1 Egyetem Square, Debrecen, 4010, Hungary, - - 2014
To visualize characteristic chromatin distortions we have distinguished first among regularly occurring intermediates of chromatin structures in mammalian (Indian muntjac, CHO, murine preB, rat liver, rat myeloid leukemia, K562 human erythroid leukemia) and Drosophila nuclei. Fluorescence microscopy of chromatin structures isolated from nuclei of reversibly permeable cells revealed a common ...
Chen Mei-Hsiu - - 2014
Estradiol plays an important role in the regulation of collagen metabolism. Deficiency of estradiol has been reported to be associated with the degeneration of many connective tissues. However, the association of estradiol and hypertrophy of the ligamentum flavum was seldom explored. Therefore, we studied the effects of estradiol on cultured ...
Ediger Benjamin N BN Department of Pathology and Laboratory Medicine, Children's Hospital of PhiladelphiaDepartment of Medicine and Institute for Diabetes, Obesity and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, - - 2014
Isl-1 is essential for the survival and ensuing differentiation of pancreatic endocrine progenitors. Isl-1 remains expressed in all adult pancreatic endocrine lineages; however, its specific function in the postnatal pancreas is unclear. Here we determine whether Isl-1 plays a distinct role in the postnatal β-cell by performing physiological and morphometric ...
Dermawan Josephine Kam Tai JK Lerner Research Institute/Molecular Genetics, Cleveland - - 2014
Erlotinib is a tyrosine kinase inhibitor approved for the treatment of patients with advanced non-small cell lung cancer (NSCLC). In these patients, erlotinib prolongs survival but its benefit remains modest since many tumors express wild-type (wt) epidermal growth factor receptor (EGFR) or develop a second-site EGFR mutation. To test drug ...
He Ping P Center for Advanced Therapeutic Strategies for Brain Disorders, The Roskamp Institute, Sarasota, FL 34243, - - 2014
Normally, sonic hedgehog (Shh) signaling induces high levels of Patched 1 (Ptc1) and its associated transcription factor Gli1 with genesis of specific neuronal progeny. But their roles in the neural stem cells (NSCs), including glial precursor cells (GPCs), of Alzheimer's disease (AD) are unclear. Here, we show that Ptc1 and ...
Lacerda Lourran P LP Universidade Federal do Piauí (UFPI), Núcleo de Pesquisa Aplicada a Saúde e ao Meio-Ambiente (NUPBSAM), Laboratório de Citogenética Vegetal e Animal, Campus Senador Helvídio Nunes de Barros (CSHNB), Picos, PI, - - 2014
In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout ...
Li Kai-Chun - - 2014
Head and neck squamous cell carcinoma (HNSCC) is a common cancer worldwide. Emerging evidence indicates that alteration of epigenetics might be a key event in HNSCC progression. Abnormal expression of histone methyltransferase G9a, which contributes to transcriptional repression of tumor suppressors, has been implicated in promoting cancerous malignancies. However, its ...
Bun Philippe P Macromolecular Complexes in Living Cells, Unité Mixe de Recherche 7592, Institut Jacques Monod, Centre National de la Recherche Scientifique, University Paris VII, Paris, France. Electronic address: - - 2014
Cell polarization is a fundamental biological process implicated in nearly every aspect of multicellular development. The role of cell-extracellular matrix contacts in the establishment and the orientation of cell polarity have been extensively studied. However, the respective contributions of substrate mechanics and biochemistry remain unclear. Here we propose a believed ...
Mendez M G MG Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, - - 2014
Vimentin intermediate filament expression is a hallmark of epithelial-to-mesenchymal transitions, and vimentin is involved in the maintenance of cell mechanical properties, cell motility, adhesion, and other signaling pathways. A common feature of vimentin-expressing cells is their routine exposure to mechanical stress. Intermediate filaments are unique among cytoskeletal polymers in resisting ...
Schwabe Anne A Systems Bioinformatics, VU University, Amsterdam, The - - 2014
Cell-to-cell variability in the molecular composition of isogenic, steady-state growing cells arises spontaneously from the inherent stochasticity of intracellular biochemical reactions and cell growth. Here, we present a general decomposition of the total variance in the copy number per cell of a particular molecule. It quantifies the individual contributions made ...
Ni Aiguo A University of North Carolina-Chapel Hill, Ophthalmology, Chapel Hill, North Carolina, United States ; - - 2014
Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by defined sets of transcription factors. We previously described reprogramming of monolayer-cultured adult mouse ciliary body epithelial cells (CE) cells by Oct4 and Klf4 but not with Oct4 alone. Here, we report Oct4 alone is sufficient to reprogram CE ...
Onen Hi H Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey - - 2014
The most widespread neoplasm of the pleura is malignant pleural mesothelioma (MPM) with low prevalence rate. The mechanistic target of rapamycin signaling pathway, inhibited by RAD001, was shown to be deregulated in MPM development and considered a novel target for the MPM therapy. The EF24, a curcumin analog, also affects ...
Uriu Koichiro K Theoretical Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, Japan. Electronic address: - - 2014
Collective cell movement is a crucial component of embryonic development. Intercellular interactions regulate collective cell movement by allowing cells to transfer information. A key question is how collective cell movement itself influences information flow produced in tissues by intercellular interactions. Here, we study the effect of collective cell movement on ...
Lea Michael A MA Michael A Lea, Jacob Pourat, Rupali Patel, Charles desBordes, Department of Biochemistry and Molecular Biology, New Jersey Medical School, Rutgers University, Newark, NJ 07103, United - - 2014
To determine if other molecules reported to modulate AMP-dependent protein kinase (AMPK) activity would have effects resembling those of metformin and phenformin on colon cancer cell proliferation and metabolism. Studies were performed with four human colon cancer cell lines, Caco-2, HCT116, HT29 and SW1116. The compounds that were studied included ...
Minonzio Greta G Swiss Stem Cell foundation, In Pasquée, 6925 Gentilino, - - 2014
In recent years, there has been a shift towards tissue-engineering strategies using stem cells for plastic and reconstructive surgical procedures. Therefore, it is important to develop safe and reproducible protocols for the extraction of adipose-derived stromal cells (ASCs) to allow cells to be stored in liquid nitrogen for future needs. ...
Ladkau Nadine N Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund University, Emil-Figge-Straße 66, 44227 Dortmund, - - 2014
Whole-cell biocatalysis has emerged as an important tool for the synthesis of value-added fine and bulk chemicals as well as pharmaceuticals. Especially, the rapid development of recombinant DNA technologies resulted in a shift from the exploitation of natural enzymes and pathways to the design of recombinant cell factories comprising heterologous ...
Chen Y Y Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610000, - - 2014
PIWIL2 is widely expressed in various tumours and implicated in playing a role in tumourigenesis. For a more thorough study of PIWIL2 functions in tumour cells, we aimed to establish PIWIL2-specific transcript knock-down/knockout HepG2 cell lines using transcription activator-like effector nuclease (TALEN) technology. Furthermore, we proposed to use the cell ...
Thompson Gemma C GC Laboratory of Molecular Neuroscience, The Brain and Mind Research Institute, The University of Sydney, Camperdown, NSW, 2050 - - 2014
Cell segmentation and counting is often required in disciplines such as biological research and medical diagnosis. Manual counting, although still employed suffers from being time consuming and sometimes unreliable. As a result, several automated cell segmentation and counting methods have been developed. A main component of automated cell counting algorithms ...
Roth Alexander D AD William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, - - 2014
Aligned fibers have been shown to facilitate cell migration in the direction of fiber alignment while oxygen (O2 )-carrying solutions improve the metabolism of cells in hypoxic culture. Therefore, U251 aggregate migration on poly(ε-caprolactone) (PCL) aligned fibers was studied in cell culture media supplemented with the O2 storage and transport ...
Fujita Koji K Endocrine Research Unit and Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, - - 2014
There is increasing evidence that osteocytes regulate multiple aspects of bone remodeling through bidirectional communication with osteoblasts. This is potentially mediated through cell-cell contact via osteocytic dendritic processes, through the activity of secreted factors, or both. To test whether cell-cell contact affects gene expression patterns in osteoblasts and osteocytes, we ...
Papo Michele Boscolo MB Dipartimento di Biomedicina Comparata e Alimentazione, Università degli Studi di Padova, Agripolis, Viale dell'Università, 16 - 35020 Legnaro (PD), - - 2014
Benzo(a)pyrene is an important polycyclic aromatic hydrocarbon (PAH) commonly present in the marine environment and responsible for carcinogenic, teratogenic and mutagenic effects in various animal species. In the present study, we investigated by both histochemical and immunohistochemical approaches the effect of an acute exposure to different concentrations of B(a)P in ...
Li Qingling Q Department of Dermatology; Xiangya Hospital, Central South University, Changsha, 410008, - - 2014
Although laboratory studies have implicated the high mobility group box 1 (HMGB1) in melanoma, its clinical relevance remains unclear. We analyzed nearly 100 cases of human melanoma and found that HMGB1 was highly overexpressed in melanoma samples relative to normal skin and nevi tissues. Significantly, higher levels of HMGB1 correlated ...
Vaitkuviene Aida A Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania; Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, - - 2014
The aim of this study was to assess the impact of nanocrystalline diamond (NCD) thin coatings on neural cell adhesion and proliferation. NCD was fabricated on fused silica substrates by microwave plasma enhanced chemical vapor deposition method. Different surface terminations were performed through exposure to reactive hydrogen and by UV ...
Albert Joan J Departament de Química Inorgànica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain; Institut de Biomedicina (IBUB), Universitat de Barcelona, Barcelona, Spain. Electronic address: - - 2014
Twelve cyclometallated palladium(II) complexes containing primary aromatic amines [benzylamine (a), (R)-1-(1-naphthyl)ethylamine (b) and 2-phenylaniline (c)] as anionic bidentate (C,N)(-) ligands have been evaluated against a panel of human adenocarcinoma cell lines (A549 lung, MDA-MB231 and MCF7 breast, and the cisplatin resistant HCT116 colon). The results revealed a remarkable antiproliferative activity ...
Liu Yang-Sui YS Yang-Sui Liu, Huan-Song Li, Dun-Feng Qi, Jun Zhang, Xin-Chun Jiang, Kui Shi, Xiao-Jun Zhang, Xin-Hui Zhang, The Central Hospital of Xuzhou City, Xuzhou Hospital Affiliated to Medical College of Southeast University, Xuzhou 221009, Jiangsu Province, - - 2014
To investigate the effect of zinc protoporphyrin IX on the response of hepatoma cells to cisplatin and the possible mechanism involved. Cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was determined by a flow cytometric assay. Western blotting was used to measure protein expression. Heme oxygenase (HO)-1 activity was ...
Boutin Camille C Institute of Neuroscience, Developmental Neurobiology, Université catholique de Louvain, B1200 Brussels, - - 2014
In the nervous system, cilia dysfunction perturbs the circulation of the cerebrospinal fluid, thus affecting neurogenesis and brain homeostasis. A role for planar cell polarity (PCP) signaling in the orientation of cilia (rotational polarity) and ciliogenesis is established. However, whether and how PCP regulates cilia positioning in the apical domain ...
Wawer Mathias J MJ Center for the Science of - - 2014
High-throughput screening has become a mainstay of small-molecule probe and early drug discovery. The question of how to build and evolve efficient screening collections systematically for cell-based and biochemical screening is still unresolved. It is often assumed that chemical structure diversity leads to diverse biological performance of a library. Here, ...
Liu Hongyi H Department of Surgical Oncology, General Hospital of Chinese People's Liberation Army, No. 28, Fuxing Rd, 100853, Beijing, - - 2014
The purposes of this study were to determine the expression profile of Raf kinase inhibitor protein (RKIP) in human gastric cancer cells and its effect on the biological characteristics of SGC-7901 cell lines, to examine the modulatory effect of microRNA-224 (miR-224) on RKIP. The research will provide novel strategies for ...
Yang Weihan W State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
Abnormal expression of solute carrier family 34 (sodium phosphate), member 2 (SLC34A2) in the lung may induce abnormal alveolar type II (AT II) cells to transform into lung adenocarcinoma cells, and may also be important in biological process of lung adenocarcinoma. However, at present, the effects and molecular mechanisms of ...
Sooresh Aishwarya A Department of Materials Science and Engineering, Texas A&M University, College Station, TX , United States - - 2014
Abstract Development of new methods and compounds to eradicate insect vectors are desperately needed. To that end, our team has previously described the synthesis and characterization of a conjugate comprised of a silver nanoparticle core encapsulated by the pyrethroid pesticide, deltamethrin (pesticide encapsulated silver nanoparticle termed "PENS"). For this current ...
Liu Yu Y 1School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan, P. R. - - 2014
The mammalian target of rapamycin (mTOR), a phosphoinositide-3-kinase-related protein kinase, acts as a rheostat capable of integrating a variety of environmental cues in the form of nutrients, energy, and growth factors to modulate organismal processes and homeostasis. Recently, there is a growing appreciation of mTOR in adaptive immunity for its ...
Tiwari Reeta R Department of Life Sciences, University of Mumbai , Mumbai , India , - - 2014
Betulinic acid (BA) has been shown to cause apoptosis in neuroblastoma and melanoma cell lines. We evaluated the cytotoxicity of BA in two breast cancer cell lines MCF-7 and T47D differing in their p53 status. Treatment with BA resulted in a dose dependent inhibition of cell proliferation and induction of ...
Tanaka Kosei K Organization of Advanced Science and Technology, Kobe University; Kobe, - - 2014
Some rare inositol stereoisomers are known to exert specific health-promoting effects, including scyllo-inositol (SI), which is a promising therapeutic agent for Alzheimer disease. We recently reported a Bacillus subtilis cell factory that performed the efficient production of SI from the cheapest and most abundant isomer myo-inositol (MI). In the cell ...
Ferreira Ana Rita AR Health Sciences Department, University of Aveiro , Aveiro , Portugal - - 2014
Abstract Epithelial cells form the epithelium, one of the basic tissues of the human body. These cells present specializations from tissue to tissue, determining different structures and functions. Tissues formed by epithelial cells are characterized by the few extracellular matrix found between adjacent cells. In this way, to preserve tissue ...
Fernandes A R AR Cellular and Neural Engineering Group, Institute for Science and Technology in Medicine, Keele University, Keele, Staffordshire, UK. - - 2014
Evaluating the uptake and handling of biomedically relevant nanoparticles by cells of the nervous system critically underpins the effective use of nanoparticle platforms for neuro-regenerative therapies. The lack of biologically relevant and 'neuromimetic' models for nanomaterials testing (that can simulate the cellular complexity of neural tissue) currently represents a bottleneck. ...
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