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Xue Rui R Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, - - 2014
A series of cardiac glycosides were isolated and identified from the anti-tumor fraction of root of Streptocaulon juventas in previous studies. In the present research, the cytotoxic activities of the 43 cardiac glycosides on three cell lines, human lung A549 adenocarcinoma cell, large cell lung cancer NCI-H460 cell and normal ...
Stokich Blake B Department of Biological Science, Eastern Illinois University, Charleston, Il - - 2014
A simple method to cryogenically preserve hepatocyte monolayers is currently not available but such a technique would facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. We investigated the effect of trehalose and dimethyl sulfoxide (Me2SO) in cryopreservation of human hepatocellular carcinoma (HepG2) cells ...
Sahin Başak B Department of Biomedical Engineering, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2V2, - - 2014
Cutaneous T-cell lymphomas (CTCLs) arise from specific molecular aberrations that lead to uncontrolled cell proliferation. RNA interference (RNAi) with short interfering RNAs (siRNAs) is a feasible approach to interrupt aberrant signal processing in CTCL cells, but functional biomaterial carriers are needed to effectively deliver siRNAs intracellularly. Towards this goal, we ...
Filla Mark S MS Department of Ophthalmology & Visual Sciences, Medical Science Center, 1300 University Ave., Madison, WI 53706. Electronic address: - - 2014
In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human ...
Gopalan Vinod V Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith University, Gold Coast, Queensland, - - 2014
miR-498 is a non-coding RNA located intergenically in 19q13.41. Due to its predicted targeting of several genes involved in control of cellular growth, we examined the expression of miR-498 in colon cancer cell lines and a large cohort of patients with colorectal adenocarcinoma. Two colon cancer cancer cell lines (SW480 ...
Pravin Narayanaperumal N Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India. Electronic address: - - 2014
A series of carboplatin type Cu(II) and Zn(II) metalloinsertors (1-8) having β-diketone analogues and biologically significant cyclobutane-1,1-dicarboxylic acid have been synthesized and characterized by spectral and analytical methods. The binding and cleavage propensity of these metalloinsertors on DNA and their cytotoxic effects in live cells have been explored. From the ...
Choi Jae Sue JS Department of Food and Life Science, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of - - 2014
Obesity is a complex, multifactorial, and chronic disease that increases the risk for type 2 diabetes, coronary heart disease and hypertension, and has become a major worldwide health problem. Developing novel anti-obesity drugs from natural products is a promising solution to the global health problem of obesity. While screening anti-obesity ...
Shutova Maria S MS Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, - - 2014
Class II myosins generate contractile forces in cells by polymerizing into bipolar filaments and pulling on anchored actin filaments. Nonmuscle myosin II (NMII) plays central roles during cell adhesion, migration, cytokinesis, and tissue morphogenesis. NMII is present in virtually all mammalian cell types as tissue-specific combinations of NMIIA, NMIIB, and ...
Chowdhury Sayan Mullick SM Department of Biomedical Engineering, Stony Brook University, Stony Brook, - - 2014
We report use of PEG-DSPE coated oxidized graphene nanoribbons (O-GNR-PEG-DSPE) as agent for delivery of anti-tumor drug Lucanthone (Luc) into Glioblastoma Multiformae (GBM) cells targeting base excision repair enzyme APE-1 (Apurinic endonuclease-1). Lucanthone, an endonuclease inhibitor of APE-1, was loaded onto O-GNR-PEG-DSPEs using a simple non-covalent method. We found its ...
Chung Yuan-Chiang - - 2014
In the process of epithelial mesenchymal transition EMT, the disassembly of junctional adhesion complexes such as E-cadherin is a remarkable sign during changes in cell morphology and polarity. However, E-cadherin expression is dynamic, and is regulated by the cellular endocytic system; it is also involved in cell signaling mechanisms. In ...
Manning Alyssa J AJ Department of Biochemistry, Box 357350, The University of Washington, Seattle, WA - - 2014
Epithelia form the building blocks of many tissue and organ types. Epithelial cells often form a contiguous 2-dimensional sheet that is held together by strong adhesions. The mechanical properties conferred by these adhesions allow the cells to undergo dramatic three-dimensional morphogenetic movements while maintaining cell-cell contacts during embryogenesis and post-embryonic ...
Zheng Lingling L State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China. - - 2014
An oligothiophene derivative named DR3TBDTT with high hydrophobicity was synthesized and functioned as the hole transporting material without an ion additive. 8.8% of power conversion efficiency was obtained for CH3NH3PbI3-xClx based planar solar cells with improved stability, compared to devices using Li-TFSI doped spiro-MeOTAD.
Du Yantao Y Zhejiang Provincial Key Laboratory of Pathophysiology, Medical School of Ningbo University, Zhejiang, China; Ningbo Institute of Medical Sciences, Medical School of Ningbo University, Ningbo, Zhejiang, - - 2014
MicroRNAs (miRNAs) are the class of small noncoding RNAs, and play an important role in the regulation of gene expression at the posttranscriptional level. In this study, we explored the effect of miR-129-1-3p on the growth and cell cycle of human gastric cancer cell line BGC-823. The miR-129-1-3p mimics or ...
Calastretti Angela A Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Milan, - - 2014
The critical role of PTEN in regulating the PI3K/Akt/mTOR signaling pathway raises the possibility that targeting downstream effectors of the PI3K pathway, such as Bcl-2, might be an effective anti-proliferative strategy for PTEN-deficient prostate cancer cells. Four prostate cancer cell lines (LNCaP, PC3, DU145, 22Rv1) were assayed for their levels ...
Mi W W Weis Center for Research, Danville, PA, - - 2014
Protein geranylgeranylation (GGylation) is an important biochemical process for many cellular signaling molecules. Previous studies have shown that GGylation is essential for cell survival in many types of cancer. However, the molecular mechanism mediating the cell survival effect remains elusive. In this report, we show that the Hippo pathway mediates ...
Nirogi Ramakrishna R Discovery Research, Suven Life Sciences Limited , Serene Chambers, Banjara Hills, Hyderabad , - - 2014
Abstract Metaphenoxy Benzyl Chloride (3-PBC; CAS # 53874-66-1) is a commonly used intermediate in organic synthesis and is a widely used chemical building block. There is likelihood of human contact with 3-PBC in the process of organic or pesticide synthesis and even through as impurity in the product. Further, this ...
Zhang Linwen L Department of Chemistry, W. M. Keck Institute for Proteomics Technology and Applications, The George Washington University, Washington, DC 20052, USA. - - 2014
Advances in single cell analysis techniques have demonstrated cell-to-cell variability in both homogeneous and heterogeneous cell populations strengthening our understanding of multicellular organisms and individual cell behaviour. However, additional tools are needed for non-targeted metabolic analysis of live single cells in their native environment. Here, we combine capillary microsampling with ...
Nune K C KC Biomaterials and Biomedical Engineering Research Laboratory, Center for Structural and Functional Materials, Institute for Materials Research and Innovation, University of Louisiana at Lafayette, P.O. Box 44130, Lafayette, LA, 70504, - - 2014
The cellular activity, biological response, and consequent integration of scaffold-cell construct in the physiological system are governed by the ability of cells to adhere, proliferate, and biomineralize. In this regard, we combine cellular biology and materials science and engineering to fundamentally elucidate the interplay between cellular activity and interconnected three-dimensional ...
Broustas Constantinos G CG Center for Radiological Research, Columbia University College of Physicians and Surgeons, New York, New - - 2014
Mouse embryonic stem cells null for Rad9 are sensitive to deleterious effects of ionizing radiation exposure. Likewise, integrin β1 is a known radioprotective factor. Previously, we showed that RAD9 downregulation in human prostate cancer cells reduces integrin β1 protein levels and ectopic expression of Mrad9 restores inherent high levels. We ...
Liu Yang Y Key Laboratory of Microsystems and Microstructures Manufacturing, Ministry of Education, Harbin Institute of Technology, Harbin, 150080, China. shaoqinliu@hit.edu.cn - - 2014
A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode ...
Reissmann Siegmund S Friedrich Schiller University, Biological and Pharmaceutical Faculty, Institute of Biochemistry and Biophysics, Dornburger Strasse 25, 07743, Jena, Germany; Jena Bioscience GmbH, Loebstedter Strasse 80, 07749, Jena, - - 2014
The penetration of polar or badly soluble compounds through a cell membrane into live cells requires mechanical support or chemical helpers. Cell-penetrating peptides (CPPs) are very promising chemical helpers. Because of their low cytotoxicity and final degradation to amino acids, they are particularly favored in in vivo studies and for ...
Kim Jaeyun J School of Engineering and Applied Sciences and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, 01238, USA; School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Republic of - - 2014
Three-dimensional macroporous scaffolds have extensively been studied for cell-based tissue engineering but their use is mostly limited to mechanical support for cell adhesion and growth on the surface of macropores. Here, a templated fabrication method is described to prepare cell-friendly inverse opal-like hydrogels (IOHs) allowing both cell encapsulation within the ...
Wang Sz S Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu, 210009, - - 2014
Intervertebral disc degeneration (IDD) is a chronic, complex process associated with low back pain; mechanisms of its occurrence have not yet been fully elucidated. Its process is not only accompanied by morphological changes, but also by systematic changes in its histological and biochemical properties. Many cellular and molecular mechanisms have ...
Iyengar Garud G Industrial Engineering and Operations Research, Columbia University, New York, NY - - 2014
Signaling receptors on the cell surface are mobile and have evolved to efficiently sense and process mechanical or chemical information. We pose the problem of identifying the optimal strategy for placing a collection of distributed and mobile sensors to faithfully estimate a signal that varies in space and time. The ...
Le Anne A Department of Pathology and Oncology,Graduate Program in Pathobiology, dangvchi@exchange.upenn.edu - - 2014
Although aerobic glycolysis provides an advantage in the hypoxic tumor microenvironment, some cancer cells can also respire via oxidative phosphorylation. These respiring ("non-Warburg") cells were previously thought not to play a key role in tumorigenesis and thus fell from favor in the literature. We sought to determine whether subpopulations of ...
Hart Jonathan R JR Departments of Molecular and Experimental Medicine - - 2014
In a fluorescence polarization screen for the MYC-MAX interaction, we have identified a novel small-molecule inhibitor of MYC, KJ-Pyr-9, from a Kröhnke pyridine library. The Kd of KJ-Pyr-9 for MYC in vitro is 6.5 ± 1.0 nM, as determined by backscattering interferometry; KJ-Pyr-9 also interferes with MYC-MAX complex formation in ...
Kadzik Rachel S RS Departments of Cell and Developmental Biology - - 2014
Changing the morphology of a simple epithelial tube to form a highly ramified branching network requires changes in cell behavior that lead to tissue-wide changes in organ shape. How epithelial cells in branched organs modulate their shape and behavior to promote bending and sculpting of the epithelial sheet is not ...
Jha Bijay Kumar BK Department of Microbiology, Keimyung University School of Medicine, Daegu, Republic of - - 2014
Encystation of Acanthamoeba castellanii is associated with resistance to chemotherapeutic agents. Blocking the encystation could potentiate the efficacy of chemotherapeutic agents and biocides. During encystation, autophagy is highly stimulated and required for proper encystation of Acanthamoeba. In this study, the cytotoxic effect of chloroquine, a well-known autophagy inhibitory drug, was ...
Shen Xinghua X Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150081, - - 2014
Alpha-lipoic acid (α-LA) is a potent antioxidant that acts as an essential cofactor in mitochondrial dehydrogenase reactions. α-LA has been shown to possess anti-inflammatory and cytoprotective properties, and is used to improve symptoms of diabetic neuropathy. However, the role of α-LA in stem cell differentiation and the underlying molecular mechanisms ...
Limmer Stefanie S Institut für Neuro- und Verhaltensbiologie, Badestrasse 9, 48149 Münster, - - 2014
Reporting in this issue of Developmental Cell, Spéder and Brand (2014) show that gap junctions are required in blood-brain barrier glial cells to reactivate proliferation of quiescent neuroblasts. Gap junctions allow synchronous Ca(2+) waves and control insulin-like protein Dipl6 expression and secretion to trigger neuroblast division.
Mendes Raquel V RV Instituto de Medicina Molecular e Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, - - 2014
The stereotypic left-right (LR) asymmetric distribution of internal organs is due to an asymmetric molecular cascade in the lateral plate mesoderm (LPM) that is originated at the embryonic node. In chicken embryos, molecular asymmetries at Hensen's node are created by leftward cell movements that occur transiently. What terminates these movements, ...
Lei Shaorong S Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. - - 2014
MacroH2A is the most frequently altered histone, which participates in cancer progression. Increasing evidence demonstrates that cancer progression could be regulated by macroH2A by affecting the cell cycle. In the present study, it was demonstrated that macroH2A suppresses melanoma cell progression and the molecular mechanisms underlying this process were examined. ...
Li Jizong J Animal Infectious Disease Laboratory, Ministry of Agriculture, Jiangsu Co-operation and Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, - - 2014
A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1-2 in Dulac cells were 100-fold ...
Caro-Astorga J J Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga - Andalucia Tech, 29071 Málaga, Spain; CIBER de Enfermedades Raras (CIBERER), 29071 Málaga, - - 2014
Histamine (Hia) is the most multifunctional biogenic amine. It is synthetized by histidine decarboxylase (HDC) in a reduced set of mammalian cell types. Mast cells and histaminergic neurons store Hia in specialized organelles until the amine is extruded by exocytosis; however, other immune and cancer cells are able to produce ...
Fuchigami Takao T Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan; Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, - - 2014
Ameloblastoma is an odontogenic benign tumor that occurs in the jawbone, which invades bone and reoccurs locally. This tumor is treated by wide surgical excision and causes various problems, including changes in facial countenance and mastication disorders. Ameloblastomas have abundant tumor stroma, including fibroblasts and immune cells. Although cell-to-cell interactions ...
Jahed Zeinab Z Molecular Cell Biomechanics Laboratory, Departments of Bioengineering and Mechanical Engineering, University of California Berkeley, 208A Stanley Hall, Berkeley, CA 94720-1762, - - 2014
Metallic nanopillar/nanowires are emerging as promising platforms for biological applications, as they allow for the direct characterization and regulation of cell function. Herein we study the response of cells to a versatile nanopillar platform. Nanopillar arrays of various shape, size, and spacing and different nanopillar-substrate interfacial strengths were fabricated and ...
Di Palma Serena S Institute of Molecular Life Sciences, University of Zürich, Zürich, - - 2014
The development of new biotechnologies for the analysis of individual cells in heterogeneous populations is an important direction of life science research. This review provides a critical overview of relevant and recent advances in the field of single-cell mass cytometry, focusing on the latest applications in the study of cell ...
Piao Songzhe S Department of Urology, Seoul National University Hospital, Seoul, Republic of Korea; Department of Urology, Yanbian University Hospital, Yanji, Jilin Province, - - 2014
To evaluate the in vitro and in vivo effects of escin on human castration-resistant prostate cancer (CRPC) cells, PC-3 and DU-145. The inhibition of cell proliferation and its mechanism were assessed through a cytotoxicity assay, flow cytometry, and Western blot. The in vivo efficacy of escin in CRPC cells was assessed using a ...
Schwab Carlton L CL Department of Obstetrics Gynecology and Reproductive Sciences, Yale School of Medicine, 333 Cedar Street, New Haven, CT - - 2014
Uterine serous carcinoma (USC) represents an aggressive variant of endometrial cancer and accounts for a large proportion of deaths annually. HER2/neu amplification is associated with USC in approximately 30-35% of cases. The objective of this study was to determine the sensitivity of a panel of primary USC cell lines to ...
Tafavogh Siamak - - 2014
Neuroblastoma Tumor (NT) is one of the most aggressive types of infant cancer. Essential to accurate diagnosis and prognosis is cellular quantitative analysis of the tumor. Counting enormous numbers of cells under an optical microscope is error-prone. There is therefore an urgent demand from pathologists for robust and automated cell ...
Dragoi Ana-Maria AM Department of Microbial Pathogenesis, Yale School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut, - - 2014
Shigella flexneri is an intracellular pathogen that disseminates in the intestinal epithelium by displaying actin-based motility. We found that although S. flexneri displayed comparable actin-based motilities in the cytosols of HeLa229 and HT-29 epithelial cell lines, the overall dissemination process was much more efficient in HT-29 cells. Time-lapse microscopy demonstrated ...
Song Wei-Tao WT Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008, - - 2014
Glaucoma is one of the leading eye diseases due to the death of retinal ganglion cells. Increasing evidence suggests that retinal Müller cells exhibit the characteristics of retinal progenitor cells and can differentiate to neurons in injured retinas under certain conditions. However, the number of ganglion cells differentiated from retinal ...
Russell E G EG School of Biochemistry and Cell Biology, University College Cork, College Road, Cork, - - 2014
A panel of novel ellipticine isomers were designed and synthesised with the aim of evaluating their anti-cancer effects on selected leukaemia cell lines. A preliminary NCI 60-cell screen demonstrated that these compounds display promising anti-tumour activity across a number of different cell types. We have consequently examined the effect of ...
Liu Chao C Department of Cariology and Endodontics, Institue and Hospital of Stomatology, Nanjing University Medical School, Nanjing, 210008, - - 2014
We evaluated the efficiency of transfecting adenoviral vectors encoding enhanced green fluorescent protein (AdCMV-EGFP) into rat submandibular gland cells and the effects of gene transfer on cell proliferation and secretory function. Isolated submandibular gland cells were transfected with different titers (or multiplicity of infection, MOI) of AdCMV-EGFP. The transfection efficiency ...
Schönhofen Patrícia P Department of Biochemistry, Laboratory of Cellular Biochemistry, ICBS/UFRGS, Porto Alegre, RS, 90035-003, - - 2014
Cannabidiol (CBD), one of the most abundant Cannabis sativa-derived compounds, has been implicated with neuroprotective effect in several human pathologies. Until now, no undesired side effects have been associated with CBD. In this study, we evaluated CBD's neuroprotective effect in terminal differentiation (mature) and during neuronal differentiation (neuronal developmental toxicity ...
Geng Ya-di YD State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, - - 2014
Icariside II is considered one of the most important natural flavonoids with multiple bioactivities from traditional Chinese medicine Yin Yanghuo (YYH) or Horny Goat Weed (Epimedium koreanum Nakai). Previous studies show that Icariside II exhibits potent cytotoxicity against a broad spectrum of human cancer cells through various signaling transduction pathways. ...
Ou Young Y Cumming School of Medicine, Department of Biochemistry and Molecular Biology, University of Calgary, 406 HMRB, 3330 Hospital Drive NW, AB, T2N 4N1, Calgary, - - 2014
In vertebrates, a variety of cell types generate a primary cilium. Cilia are implicated in determination and differentiation of a wide variety of organs and during embryonic development. However, there is little information on the presence or function of primary cilia in the mammalian testis. Therefore, the objective of this ...
Harrath Abdel Halim AH Zoology Department College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia, - - 2014
Ex-fissiparous planarians produce infertile cocoons or, in very rare cases, cocoons with very low fertility. Here, we describe the features of programmed cell death (PCD) occurring in the hyperplasic ovary of the ex-fissiparous freshwater planarian Dugesia arabica that may explain this infertility. Based on TEM results, we demonstrate a novel ...
Kumbıçak Umit U Department of Molecular Biology and Genetics, Faculty of Science and Art, Nevşehir University, 50300, Nevşehir, - - 2014
In the present study, in vitro cytotoxic and genotoxic effect of copper-zinc alloy nanoparticles (Cu-Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ...
Huang Huiyi H Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, - - 2014
The purpose of this study was to investigate the relationship between N-acetylglucosaminyltransferase V (GnT-V) and radiation sensitivity of prostate cancer (PCa) cells both in vitro and in vivo. Firstly, the GnT-V expression was studied in 84 cases of PCa tissues, in which higher level of GnT-V was detected more frequently ...
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