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Schweigmann H H Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, 35392 Giessen, - - 2014
16α-hydroxy-dehydroepiandrosterone sulfate (16α-OH-DHEAS) mainly originates from the fetus and serves as precursor for placental estriol biosynthesis. For conversion of 16α-OH-DHEAS to estriol several intracellular enzymes are required. However, prior to enzymatic conversion, 16α-OH-DHEAS must enter the cells by carrier mediated transport. To identify these carriers, uptake of 16α-OH-DHEAS by the ...
Fan Rong R Department of Gastroenterology, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin Er Rd, Shanghai, 200025, - - 2014
To detect the expressions of microRNA-218 (miR-218) in an imatinib mesylate-sensitive human gastrointestinal stromal tumor (GIST) cells (GIST882) and an imatinib mesylate-resistant cell line (GIST430) and explore the roles of miR-218 and GIST cells in the sensitivity of gastrointestinal stromal tumor to imatinib mesylate and its potential signaling pathways, with ...
Lammel Tobias T Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Medio Ambiente, Carretera de la Coruña Km 7.5, 28040, Madrid, - - 2014
Graphene and its derivatives are an emerging class of carbon nanomaterial with great potential for a broad range of industrial and consumer applications. However, their increasing production and use is expected to result in release of nano-sized graphene platelets into the environment, where they may interact with chemical pollutants modifying ...
Jantas D D Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12 Street, PL 31-343 Krakow, Poland. Electronic address: - - 2014
Recent studies have documented that metabotropic glutamate receptors from group II and III (mGluR II/III) are a potential target in the symptomatic treatment of Parkinson's disease (PD), however, the neuroprotective effects of particular mGluR II/III subtypes in relation to PD pathology are recognized only partially. In the present study, we ...
Scheiblich Hannah H Division of Cell Biology, University of Veterinary Medicine Hannover, Germany. Electronic address: - - 2014
Microglia are the resident immune cells of the brain, which become rapidly activated and migrate to the site of insult in brain infection and disease. Activated microglia generate large amounts of the highly reactive messenger molecule nitric oxide (NO). NO is able to raise cyclic GMP levels via binding to ...
Yaniv Yael Y Laboratory of Cardiovascular Science, Biomedical Research Center, Intramural Research Program, National Institute on Aging, NIH, Baltimore, Maryland, USA.; Biomedical Engineering Faculty, Technion-IIT, Haifa, Israel.. Electronic address: - - 2014
A reduction of complexity of heart-beat interval variability (BIV) that is associated with an increased morbidity and mortality in cardiovascular disease states is thought to derive from the balance of sympathetic and parasympathetic neural impulses to the heart. But rhythmic clock-like behavior intrinsic to pacemaker cells within the sinoatrial node ...
Xu Peng-Fei PF Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, - - 2014
Development of vertebrate embryos involves tightly regulated molecular and cellular processes that progressively instruct proliferating embryonic cells about their identity and behavior. Whereas numerous gene activities have been found to be essential during early embryogenesis, little is known about the minimal conditions and factors that would be sufficient to instruct ...
Wang Junjie J Department of Applied Chemistry, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. - - 2014
Novel small-molecule hole transporting materials (HTMs) with a linear π-conjugated structure have been synthesized. The perovskite solar cell based on as the HTM exhibits an encouraging power conversion efficiency of 9.1% under AM 1.5 G (100 mW cm(-2)) illumination, which is the first demonstration of an effective perovskite solar cell ...
Serizawa Masakuni M Drug Discovery and Development Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, - - 2014
Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are effective for non-small cell lung cancers (NSCLCs) with EGFR-activating mutations. However, most responders develop resistance. Efatutazone, a novel peroxisome proliferator-activated receptor gamma (PPARγ) agonist, is currently under clinical evaluation; it has antiproliferative effects and induces cellular morphological changes and differentiation. This study ...
Toft-Kehler Anne Katrine AK Department of Neuroscience and Pharmacology, the Panum Institute, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, - - 2014
The viability of retinal ganglion cells (RGC) is essential for the maintenance of visual function. RGC homeostasis is maintained by the surrounding retinal glial cells, the Müller cells, which buffer the extracellular concentration of neurotransmitters and provide the RGCs with energy. This study evaluates if glucose-deprivation of Müller cells interferes ...
Chen Yinning Y a Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou 510-650 , - - 2014
Further chemical investigation on the marine sponge Callyspongia sp. collected from South China Sea led to the isolation of a new diketopiperazine, named callysponine A (1), as well as four known diketopiperazines, namely cyclo-(Gly-Pro) (2), cyclo-(Thr-Pro) (3), cyclo-(Ile-Pro) (4) and cyclo-(Pro-Pro) (5). The new structure was determined on the basis ...
Kandylis Panagiotis P Food Biotechnology Group, Department of Chemistry, University of Patras, GR-26500, Patras, Greece, - - 2014
A biocatalyst was prepared by immobilization of Saccharomyces cerevisiae AXAZ-1 yeast cells on potato pieces. This biocatalyst was subjected to freeze-drying, and the effect of several protective agents and storage at 5 °C, up to 9 months, on viability and fermentative activity of yeasts cells were studied. From several protective agents tested, ...
Svensson D D Department of Experimental Medical Science, Lund University, Lund, - - 2014
High levels of the antimicrobial peptide, LL-37, are detected in gingival crevicular fluid from patients with chronic periodontitis. LL-37 not only shows antimicrobial activity but also affects host-cell viability. The objective of the present study was to identify endogenous mechanisms that antagonize the detrimental effects of LL-37 on osteoblast viability, ...
Kang Yong-Kook YK Development and Differentiation Research Center, KRIBB, 111 Gwahang-ro, Yuseong-gu, Daejeon 305-806, South - - 2014
The histone methyltransferase SETDB1 contributes to the silencing of local chromatin and the target specificity appears to be determined through various proteins that SETDB1 interacts with. This fundamental function endows SETDB1 with specialized roles in embryonic cells. Keeping the genomic and transcriptomic integrity via proviral silencing and maintaining the pluripotency ...
Zheng Yong Y Hubei Key Laboratory of Genetic Regulation and Integrative Biology; College of Life Sciences; Huazhong Normal University; Wuhan, PR China; Department of Biology; University of Missouri; St. Louis, MO USA; Donald Danforth Plant Science Center; St. Louis, MO - - 2014
Patatin-containing phospholipase A (pPLA) hydrolyzes membrane glycerolipids, producing free fatty acids and lysoglycerolipids. Ten pPLAs in the Arabidopsis thaliana genome are grouped into 3 subfamilies, and pPLAIIIs differ from pPLAI and IIs in their catalytic motifs and overexpression (OE) of pPLAIIIs reduces cell elongation and cellulose content. To probe the ...
Yamamoto Ryohei R Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Ourai Kita, Izumisano, Osaka 598-8531, - - 2014
Oxidative base damage occurs spontaneously due to reactive oxygen species generated as byproducts of respiration and other pathological processes in mammalian cells. Many oxidized bases are mutagenic and/or toxic, and most are repaired through the base excision repair pathway. Human endonuclease VIII-like protein 1 (hNEIL1) is thought to play an ...
Chen Xueran X Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Jinan, Shandong, China ; - - 2014
The roles of DHHC-containing proteins in embryonic cell fate specification are not well defined, nor are the underlying mechanisms of their activity well understood. Here, we compared the embryonic function of zinc finger DHHC-type containing (Zdhhc13) in zebrafish embryos and in an <i>in vitro </i>cell model. Zdhhc13, a critical regulator ...
Seager Anna L AL DNA Damage Research Group, Institute of Life Science, College of Medicine, Swansea University, Swansea SA2 8PP, - - 2014
Micronucleus (MN) induction is an established cytogenetic end point for evaluating structural and numerical chromosomal alterations in genotoxicity testing. A semi-automated scoring protocol for the assessment of MN preparations from human cell lines and a 3D skin cell model has been developed and validated. Following exposure to a range of ...
Lewis Christina L CL Food Protection Team, Combat Feeding Directorate, US Army Natick Soldier Research, Development, and Engineering Center, Natick, MA 01760, - - 2014
Monitoring cell growth and measuring physical features of foodborne pathogenic bacteria is important for better understanding the conditions in which these organisms survive and proliferate. To address this challenge, buoyant masses of live and dead, Escherichia coli O157:H7 and Listeria innocua were measured using, Archimedes, a commercially available suspended microchannel ...
Xiao Jie J Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, - - 2014
Our previous studies have shown that quercetin inhibits Cox-2 and Bcl-2 expressions, and induces human leukemia HL-60 cell apoptosis. In order to investigate the role of AMP-activated protein kinase (AMPK) on quercetin-induced apoptosis of HL-60 cells, we used flow cytometry to detect cell apoptosis. The expressions of LKB1, phosphorylated AMPK ...
Feng Yuxiong Y 1Cancer Program, Whitehead Institute for Biomedical - - 2014
Epithelial-to-mesenchymal transition (EMT) promotes both tumor progression and drug resistance, yet few vulnerabilities of this state have been identified. Using selective small molecules as cellular probes, we show that induction of EMT greatly sensitizes cells to agents that perturb endoplasmic reticulum (ER) function. This sensitivity to ER perturbations is caused ...
Wang Ting - - 2014
By definition, all epithelial cells have apical-basal polarity, but it is unclear how epithelial polarity is acquired and how polarized cells engage in tube formation. Here, we show that hepatocyte polarization is linked to cytokinesis using the rat hepatocyte cell line Can 10. Before abscission, polarity markers are delivered to ...
Tokuda Emi E Integrated Center for Mass Spectrometry, Kobe University Graduate School of - - 2014
Downregulation of cell-cell adhesion and upregulation of cell migration play critical roles in the conversion of benign tumors to aggressive invasive cancers. In this study, we show that changes in cell-cell adhesion and cancer cell migration/invasion capacity depend on the level of phosphatidylinositol 4-phosphate (PI4P) in the Golgi apparatus in ...
Calista D D Department of Dermatology, "M. Bufalini" Hospital, Viale Ghirotti, 286, 47522, Cesena, - - 2014
Topical photodynamic therapy (PDT) consists of the local administration of a photosensitizer, which is accumulated and retained in cancerous cells, but not in normal cells, and activated by exposure to specific wavelengths of light. Sensitising drugs, usually precursors of haematoporphyrin, absorb photons of light and transfer their energy to the ...
Zhai Bo B The Hepatosplenic Surgery Center, The First Affiliated Hospital of Harbin Medical - - 2014
Sorafenib is the standard first-line systemic drug for advanced hepatocellular carcinoma (HCC), but the acquired resistance to sorafenib results in limited benefits. Activation of Akt is thought to be responsible for mediating the acquired resistance to sorafenib. The present study aims to examine the underlying mechanism and seek potential strategies ...
Ogata Makiko M Diabetes Center, Tokyo Women's Medical University, Tokyo, Japan. Electronic address: - - 2014
Glucagon-like peptide (GLP)-1 analog based therapies are used not only for their insulinotropic effects, but also for their pleiotropic effects that improve pancreatic β cell function. Liraglutide is a long acting derivative of human GLP-1(7-37), which is a cleavage product encompassing amino acids 7-37 of GLP-1. In this study, we ...
Wu Deyao D Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng First People's Hospital, Yancheng, Jiangsu 224001, P.R. - - 2014
microRNA (miRNA)-133b has been revealed to be downregulated in head and neck/oral, bladder, human non‑small cell lung, colorectal and esophageal squamous cell cancer. The present study examined the expression of miR‑133b in renal cell carcinoma (RCC) cell lines and the effects of miRNA‑133b on RCC cell proliferation, migration and invasion. ...
Chen Bingpeng B Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. - - 2014
NIN1/RPN12 binding protein 1 homolog (Saccharomyces cerevisiae) (NOB1) encodes a chaperone protein that joins the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the biogenesis of the 26S proteasome, which plays a role in maintaining cellular homeostasis by controlling protein degradation. In order to investigate the role ...
Yu Guanghui G a National Engineering Laboratory for Animal Breeding, Ministry of Agricultural Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , China Agricultural University , Beijing , - - 2014
The nucleus and mitochondria are on correlative dependence; they interact in the process of protein transportation and energy metabolism. The compatibility of nucleus and mitochondria is essential for interspecies somatic cell nuclear transfer (iSCNT) and xenomitochondrial cybrid. In order to test the compatibility of nucleus and mitochondria among human, mouse, ...
Sarsour Ehab H EH Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242-1181, - - 2014
Cancer is an age-associated disease. Although the mechanisms of age-associated increase in cancer incidence are not completely understood, it is believed that the tumor stromal environment significantly influences epithelial malignancy. Fibroblasts are a major cell type in the stroma and, under normal conditions, fibroblasts reside in the quiescent state. Cellular ...
Poudineh Mahla M Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON M5S 3G4, Canada. - - 2014
Biomarkers such as proteins and nucleic acids released from human cells, bacteria, and viruses offer a wealth of information pertinent to diagnosis and treatment ranging from cancer to infectious disease. The release of these molecules from within cells is a crucial step in biomarker analysis. Here we show that purely ...
Rao Namrata V NV Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, - - 2014
Vibrio vulnificus, an important food-borne pathogen, is known to enter viable but nonculturable (VBNC) state under low temperature and low nutrition stress conditions. Present study examined the time required for induction of VBNC state and temperature which induces resuscitation of V. vulnificus YJ016. The change in cell morphology and gene ...
Koste L L III Medical Clinic at the University Medical Center of the Johannes Gutenberg University Mainz, Mainz, - - 2014
Adoptive T-cell transfer for cancer immunotherapy requires genetic modification of T cells with recombinant T-cell receptors (TCRs). Amphotropic retroviral vectors (RVs) used for TCR transduction for this purpose are considered safe in principle. Despite this, TCR-coding and packaging vectors could theoretically recombine to produce replication competent vectors (RCVs), and transduced T-cell ...
Bertassoni Luiz E LE Biomaterials Research Unit, Faculty of Dentistry, University of Sydney, Sydney, NSW 2010, Australia. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, - - 2014
Fabrication of three dimensional (3D) organoids with controlled microarchitectures has been shown to enhance tissue functionality. Bioprinting can be used to precisely position cells and cell-laden materials to generate controlled tissue architecture. Therefore, it represents an exciting alternative for organ fabrication. Despite the rapid progress in the field, the development ...
Hamid Qudus Q Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA, - - 2014
Three-dimensional tissue platforms are rapidly becoming the method of choice for quantification of the heterogeneity of cell populations for many diagnostic and drug therapy applications. Microfluidic sensors and the integration of sensors with microfluidic systems are often described as miniature versions of their macro-scale counterparts. This technology presents unique advantages ...
Szymanski John M JM Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15219, - - 2014
Natural biopolymers such as alginate have become important materials for a variety of biotechnology applications including drug delivery, cell encapsulation and tissue engineering. This expanding use has spurred the development of new approaches to engineer these materials at the nano- and microscales to better control cell interactions. Here we describe ...
van Gestel Jordi J 1] Theoretical Biology Group, Centre for Ecological and Evolutionary Studies, University of Groningen, Groningen, The Netherlands [2] Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The - - 2014
In nature, most bacteria live in surface-attached sedentary communities known as biofilms. Biofilms are often studied with respect to bacterial interactions. Many cells inhabiting biofilms are assumed to express 'cooperative traits', like the secretion of extracellular polysaccharides (EPS). These traits can enhance biofilm-related properties, such as stress resilience or colony ...
Gupta Archana - - 2014
Exosomes are membrane-bound nanovesicles that are shed by cells of various lineages under normal as well as pathological conditions. Previously thought to be 'extracellular debris', exosomes have recently generated immense interest following their discovery as mediators of intercellular communication by delivering functional proteins, mRNA transcripts as well as miRNAs to ...
Yang Qingbo Q Missouri University of Science and Technology, Rolla, MO, - - 2014
Zinc oxide (ZnO) nanoparticles (NPs) are being widely utilized in industry due to their versatile properties. The in vitro cytotoxicity findings and the potential for exposures to ZnO NP from different sources via different routes of entry into the body have raised public health concerns. Although recent studies have shown ...
Li Wei W Department of General Surgery, Jiangxi Provincial Corps Hospital, Chinese People's Armed Police Force, Nanchang, Jiangxi 330003, P.R. - - 2014
The present study examined the effects of cisplatin (DDP) on gastric carcinoma cells by inhibiting the expression of excision repair cross‑complementing 1 (ERCC1) using RNA interference (RNAi). mRNA and protein expression of ERCC1 were measured in various gastric carcinoma cell lines using reverse transcription polymerase chain reaction (RT‑PCR) and western blot analysis. ...
Eeckhout Sharon S Research Group Pteridology, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, - - 2014
Background and AimsInnovations in vegetative and reproductive characters were key factors in the evolutionary history of land plants and most of these transformations, including dramatic changes in life cycle structure and strategy, necessarily involved cell-wall modifications. To provide more insight into the role of cell walls in effecting changes in ...
Gudi Radhika R Medical University of South Carolina, United - - 2014
Centriole duplication is the process by which two new daughter centrioles are generated from the proximal end of pre-existing mother centrioles. Accurate centriole duplication is important for many cellular and physiological events including cell division and ciliogenesis. Centrosomal protein 4.1-associated protein (CPAP), centrosomal protein of 152 kDa (CEP152) and centrobin ...
Aoki-Nakano Mizuho M Research Center for Particle Therapy, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, - - 2014
We investigated the relative biological effectiveness (RBE) of therapeutic proton beams at six proton facilities in Japan with respect to cell lethality of HSG cells. The RBE of treatments could be determined from experimental data. For this purpose, we used a cell survival assay to compare the cell-killing efficiency of ...
Yamashita Makiko M Department of Neuropathology and Cell - - 2014
TDP-43 is the major component protein of inclusions found in brains of patients with amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD-TDP). However, the molecular mechanisms by which TDP-43 causes neuronal dysfunction and death remain unknown. Here we report distinct cytotoxic effects of full-length TDP-43 and its C-terminal fragment ...
Doolaanea Abd Almonem AA Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM) , Kuantan , Malaysia - - 2014
Abstract The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of ...
Yumoto Kenji K Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan, - - 2014
Monitoring single cell proliferation in vivo is difficult, but optimizing this technique is essential in order to expand our knowledge of the regulation of tumor proliferation. In this study, we used a lipophilic fluorescent dye, DiD, that rapidly and stably integrates into the phospholipid cell membrane. We cultured DiD-stained prostate ...
Satoh Daisuke D 1. Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama, - - 2014
The slit diaphragm (SD), the specialized intercellular junction between renal glomerular epithelial cells (podocytes), provides a selective-filtration barrier in renal glomeruli. Dysfunction of the SD results in glomerular diseases that are characterized by disappearance of SD components, such as nephrin, from the cell surface. Although the importance of endocytosis and ...
Lee Jea Hwang JH Korea University, Korea, Republic - - 2014
During endoplasmic reticulum (ER)-associated degradation, p97(VCP) is recruited to the ER membrane through interactions with transmembrane proteins, such as selenoprotein S (SelS), selenoprotein K (SelK), hrd1, and gp78. SelS has a single-spanning transmembrane domain and protects cells from ER stress-induced apoptosis through interaction with p97(VCP). The cytosolic tail of SelS ...
Kwa Timothy T Department of Biomedical Engineering, University of California, Davis, Genome and Biomedical Sciences Building, 451 Health Sciences Drive Room 2619, Davis, CA 95616, United States. - - 2014
We report the development of a microsystem integrating anti-TNF-α aptasensors with vacuum-actuatable microfluidic devices that may be used to monitor intercellular communications. Actuatable chambers were used to expose to mitogen a group of ~600 cells while not stimulating another group of monocytes only 600 μm away. Co-localizing groups of cells ...
David Laurent L INSERM, UMR 1064, 44093 Nantes Cedex 1, France; Faculté de Médecine, Université de Nantes, 44035 Nantes Cedex 1, France; iPSC Core Facility, SFR F. Bonamy, Université de Nantes, France. Electronic address: - - 2014
Two studies by Sakurai et al. (2014) and Hu et al. (2014) in this issue of Cell Stem Cell add a new level of understanding to the mesenchymal-to-epithelial transition taking place during reprogramming, showing how this morphological transformation is promoted by Tet enzymes and blocked by kinase-dependent cytoskeletal organization.
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