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Limmer Stefanie S Institut für Neuro- und Verhaltensbiologie, Badestrasse 9, 48149 Münster, - - 2014
Reporting in this issue of Developmental Cell, Spéder and Brand (2014) show that gap junctions are required in blood-brain barrier glial cells to reactivate proliferation of quiescent neuroblasts. Gap junctions allow synchronous Ca(2+) waves and control insulin-like protein Dipl6 expression and secretion to trigger neuroblast division.
Mendes Raquel V RV Instituto de Medicina Molecular e Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, - - 2014
The stereotypic left-right (LR) asymmetric distribution of internal organs is due to an asymmetric molecular cascade in the lateral plate mesoderm (LPM) that is originated at the embryonic node. In chicken embryos, molecular asymmetries at Hensen's node are created by leftward cell movements that occur transiently. What terminates these movements, ...
Lei Shaorong S Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. - - 2014
MacroH2A is the most frequently altered histone, which participates in cancer progression. Increasing evidence demonstrates that cancer progression could be regulated by macroH2A by affecting the cell cycle. In the present study, it was demonstrated that macroH2A suppresses melanoma cell progression and the molecular mechanisms underlying this process were examined. ...
Li Jizong J Animal Infectious Disease Laboratory, Ministry of Agriculture, Jiangsu Co-operation and Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, - - 2014
A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1-2 in Dulac cells were 100-fold ...
Caro-Astorga J J Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga - Andalucia Tech, 29071 Málaga, Spain; CIBER de Enfermedades Raras (CIBERER), 29071 Málaga, - - 2014
Histamine (Hia) is the most multifunctional biogenic amine. It is synthetized by histidine decarboxylase (HDC) in a reduced set of mammalian cell types. Mast cells and histaminergic neurons store Hia in specialized organelles until the amine is extruded by exocytosis; however, other immune and cancer cells are able to produce ...
Fuchigami Takao T Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan; Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, - - 2014
Ameloblastoma is an odontogenic benign tumor that occurs in the jawbone, which invades bone and reoccurs locally. This tumor is treated by wide surgical excision and causes various problems, including changes in facial countenance and mastication disorders. Ameloblastomas have abundant tumor stroma, including fibroblasts and immune cells. Although cell-to-cell interactions ...
Jahed Zeinab Z Molecular Cell Biomechanics Laboratory, Departments of Bioengineering and Mechanical Engineering, University of California Berkeley, 208A Stanley Hall, Berkeley, CA 94720-1762, - - 2014
Metallic nanopillar/nanowires are emerging as promising platforms for biological applications, as they allow for the direct characterization and regulation of cell function. Herein we study the response of cells to a versatile nanopillar platform. Nanopillar arrays of various shape, size, and spacing and different nanopillar-substrate interfacial strengths were fabricated and ...
Di Palma Serena S Institute of Molecular Life Sciences, University of Zürich, Zürich, - - 2014
The development of new biotechnologies for the analysis of individual cells in heterogeneous populations is an important direction of life science research. This review provides a critical overview of relevant and recent advances in the field of single-cell mass cytometry, focusing on the latest applications in the study of cell ...
Piao Songzhe S Department of Urology, Seoul National University Hospital, Seoul, Republic of Korea; Department of Urology, Yanbian University Hospital, Yanji, Jilin Province, - - 2014
To evaluate the in vitro and in vivo effects of escin on human castration-resistant prostate cancer (CRPC) cells, PC-3 and DU-145. The inhibition of cell proliferation and its mechanism were assessed through a cytotoxicity assay, flow cytometry, and Western blot. The in vivo efficacy of escin in CRPC cells was assessed using a ...
Schwab Carlton L CL Department of Obstetrics Gynecology and Reproductive Sciences, Yale School of Medicine, 333 Cedar Street, New Haven, CT - - 2014
Uterine serous carcinoma (USC) represents an aggressive variant of endometrial cancer and accounts for a large proportion of deaths annually. HER2/neu amplification is associated with USC in approximately 30-35% of cases. The objective of this study was to determine the sensitivity of a panel of primary USC cell lines to ...
Tafavogh Siamak - - 2014
Neuroblastoma Tumor (NT) is one of the most aggressive types of infant cancer. Essential to accurate diagnosis and prognosis is cellular quantitative analysis of the tumor. Counting enormous numbers of cells under an optical microscope is error-prone. There is therefore an urgent demand from pathologists for robust and automated cell ...
Song Wei-Tao WT Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, 410008, - - 2014
Glaucoma is one of the leading eye diseases due to the death of retinal ganglion cells. Increasing evidence suggests that retinal Müller cells exhibit the characteristics of retinal progenitor cells and can differentiate to neurons in injured retinas under certain conditions. However, the number of ganglion cells differentiated from retinal ...
Russell E G EG School of Biochemistry and Cell Biology, University College Cork, College Road, Cork, - - 2014
A panel of novel ellipticine isomers were designed and synthesised with the aim of evaluating their anti-cancer effects on selected leukaemia cell lines. A preliminary NCI 60-cell screen demonstrated that these compounds display promising anti-tumour activity across a number of different cell types. We have consequently examined the effect of ...
Liu Chao C Department of Cariology and Endodontics, Institue and Hospital of Stomatology, Nanjing University Medical School, Nanjing, 210008, - - 2014
We evaluated the efficiency of transfecting adenoviral vectors encoding enhanced green fluorescent protein (AdCMV-EGFP) into rat submandibular gland cells and the effects of gene transfer on cell proliferation and secretory function. Isolated submandibular gland cells were transfected with different titers (or multiplicity of infection, MOI) of AdCMV-EGFP. The transfection efficiency ...
Schönhofen Patrícia P Department of Biochemistry, Laboratory of Cellular Biochemistry, ICBS/UFRGS, Porto Alegre, RS, 90035-003, - - 2014
Cannabidiol (CBD), one of the most abundant Cannabis sativa-derived compounds, has been implicated with neuroprotective effect in several human pathologies. Until now, no undesired side effects have been associated with CBD. In this study, we evaluated CBD's neuroprotective effect in terminal differentiation (mature) and during neuronal differentiation (neuronal developmental toxicity ...
Geng Ya-di YD State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, - - 2014
Icariside II is considered one of the most important natural flavonoids with multiple bioactivities from traditional Chinese medicine Yin Yanghuo (YYH) or Horny Goat Weed (Epimedium koreanum Nakai). Previous studies show that Icariside II exhibits potent cytotoxicity against a broad spectrum of human cancer cells through various signaling transduction pathways. ...
Huang Hai - - 2014
Several studies have shown that secreted clusterin (sCLU) up-regulation in multi-drug resistant osteosarcoma (OS) cells relates to enhanced drug resistance. Furthermore, sCLU silencing directed against sCLU induces significant reduction of cellular growth and sensitizes OS cells to chemotherapy. However, the molecular mechanisms underlying the effect of sCLU on OS cells ...
Ou Young Y Cumming School of Medicine, Department of Biochemistry and Molecular Biology, University of Calgary, 406 HMRB, 3330 Hospital Drive NW, AB, T2N 4N1, Calgary, - - 2014
In vertebrates, a variety of cell types generate a primary cilium. Cilia are implicated in determination and differentiation of a wide variety of organs and during embryonic development. However, there is little information on the presence or function of primary cilia in the mammalian testis. Therefore, the objective of this ...
Harrath Abdel Halim AH Zoology Department College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia, - - 2014
Ex-fissiparous planarians produce infertile cocoons or, in very rare cases, cocoons with very low fertility. Here, we describe the features of programmed cell death (PCD) occurring in the hyperplasic ovary of the ex-fissiparous freshwater planarian Dugesia arabica that may explain this infertility. Based on TEM results, we demonstrate a novel ...
Kumbıçak Umit U Department of Molecular Biology and Genetics, Faculty of Science and Art, Nevşehir University, 50300, Nevşehir, - - 2014
In the present study, in vitro cytotoxic and genotoxic effect of copper-zinc alloy nanoparticles (Cu-Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ...
Huang Huiyi H Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, - - 2014
The purpose of this study was to investigate the relationship between N-acetylglucosaminyltransferase V (GnT-V) and radiation sensitivity of prostate cancer (PCa) cells both in vitro and in vivo. Firstly, the GnT-V expression was studied in 84 cases of PCa tissues, in which higher level of GnT-V was detected more frequently ...
De Rybel Bert B Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the - - 2014
Coordination of cell division and pattern formation is central to tissue and organ development, particularly in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge upon tissue development. We identify a genetic network that reinforces ...
Bae Joonbeom J Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Republic of - - 2014
Lymphocyte activation gene-3 (LAG-3; CD223), a structural homolog of CD4, binds to MHC class II molecules. Recent research indicated that signaling mediated by LAG-3 inhibits T cell proliferation, and LAG-3 serves as a key surface molecule for the function of regulatory T cells. Previous reports demonstrated that the majority of ...
Song Guiyun G Department of Stem Cell Transplantation and Cellular Therapy, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, - - 2014
Clofarabine (Clo), fludarabine (Flu), and busulfan (Bu) are used in pre-transplant conditioning therapy for patients with myeloid leukemia. To further improve their efficacy in FLT3-ITD-positive AML, we investigated their synergism with sorafenib (Sor). Exposure of FLT3-ITD-positive MV-4-11 and MOLM 13 cells to [Bu+Clo+Flu+Sor] resulted in synergistic cytotoxicity; no such synergism ...
Chen B B Department of Urology and Chawnshang Chang Liver Cancer Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, - - 2014
Docetaxel-based therapy is one of the first-line options for castration-resistant prostate cancer (CRPC). However, a large proportion of CRPC patients show different extents of docetaxel resistance. The current study aims to investigate the role of testicular nuclear receptor 4 (TR4) in docetaxel resistance in CRPC. TR4 expression level in prostate ...
de Groot Martje W G D M MW Neurotoxicology Research Group, Toxicology Division, Institute for Risk Assessment Sciences (IRAS), Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.177, NL 3508 TD Utrecht, The Netherlands. Electronic address: - - 2014
Increasing exposure to extremely low frequency electromagnetic fields (ELF-EMF), generated by power lines and electric appliances, raises concern about potential adverse health effects of ELF-EMF. The central nervous system is expected to be particularly vulnerable to ELF-EMF as its function strongly depends on electrical excitability. We therefore investigated effects of ...
Cápiro Natalie L - - 2014
Contemporary microbial monitoring of aquifers relies on groundwater samples to enumerate non-attached cells of interest. One-dimensional column studies quantified the distribution of bacterial cells in solid and the aqueous phases as a function of microbial species, growth substrate availability and porous medium (i.e., Appling soil versus Federal Fine Ottawa sand ...
Matharu Zimple - - 2014
We developed cell-culture/biosensor platform consisting of aptamer-modified Au electrodes integrated with reconfigurable microfluidics for monitoring of TGF-β1 - an important inflammatory and pro-fibrotic cytokine. Aptamers were thiolated, labeled with redox reporters and self-assembled on gold surfaces. The biosensor was determined to be specific for TGF-β1 with experimental detection limit of ...
Eldhose Binil B School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala-686560, - - 2014
Despite increased use of early detection methods and more aggressive treatment strategies, the worldwide incidence of colorectal cancer is still on the rise. Consequently, it remains urgent to identify novel agents with enhanced efficacy in prevention and/or therapeutic protocols. Our studies focused on the use of Plumbagin, a natural phytochemical ...
Gires Olivier O Department of Otorhinolaryngology, Head and Neck Surgery, Grosshadern Medical Center, Ludwig-Maximilians-University of Munich, Marchioninistr. 15, 81377, Munich, Germany, - - 2014
Formation of metastasis is the most important and lethal step in cancer progression. Circulating and disseminated cancer cells (CTCs/DTCs) in blood and bone marrow are considered as potential metastases-inducing cells. Their detection and characterization has, therefore, become a field of major interest in translational and clinical research in oncology. The ...
Kim Seong Chul SC The Ohio State - - 2014
The molecular mechanisms for lung cell repair are largely unknown. Previous studies identified tripartite motif protein 72 (TRIM72) from striated muscle and linked its function to tissue repair. In this study, we characterized TRIM72 expression in lung tissues and investigated the role of TRIM72 in repair of alveolar epithelial cells. ...
Nisenholz Noam N Institute of Dental Sciences and Fritz Haber Center for Molecular Dynamics, Hebrew University of Jerusalem, 91120, Israel. - - 2014
The spreading area of cells has been shown to play a central role in the determination of cell fate and tissue morphogenesis; however, a clear understanding of how spread cell area is determined is still lacking. The observation that cell area and force generally increase with substrate rigidity suggests that ...
Li Yan Y Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, BC, Canada V5Z - - 2014
Context: Inhibin β subunits and activin receptors are expressed by human trophoblast cells. Though activin A has been shown to enhance human trophoblast cell invasion, whether two additional activin isoforms, activin B and AB, exert similar effects remains unknown. Moreover, whether the expression of mesenchymal adhesion molecule neural cadherin (N-cadherin) ...
Liu Yonghua Y Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People's Republic of - - 2014
Histone deacetylase 4 (HDAC4), a member of the class IIa HDACs subfamily, has emerged as a critical regulator of cell growth, differentiation, and migration in various cell types. It was reported that HDAC4 stimulated colon cell proliferation via repression of p21. Also, HDAC4 contributes to platelet-derived growth factor-BB-induced proliferation and ...
Lucía Zarzosa Ana A Center for Regenerative Therapies Dresden (CRTD), Technische Universität, Dresden, Fetscherstrasse 105, 01307 Dresden, - - 2014
How animals adjust the size of their organs is a fundamental, enduring question in biology. Here we manipulate the amount of neural crest (NC) precursors for the dorsal root ganglia (DRG) in axolotl. We produce embryos with an under- or over-supply of pre-migratory NC in order to find out if ...
Cerrone Federico F School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, - - 2014
A mathematically based fed-batch bioprocess demonstrated the suitability of using a relatively cheap and renewable substrate (butyric acid) for Pseudomonas putida CA-3 high cell density cultivation. Butyric acid fine-tuned addition is critical to extend the fermentation run and avoid oxygen consumption while maximising the biomass volumetric productivity. A conservative submaximal ...
Gerhardt Matthias - - 2014
The membrane and actin cortex of a motile cell can autonomously differentiate into two states, one typical of the front, the other of the tail. On the substrate-attached surface of Dictyostelium cells, dynamic patterns of front-like and tail-like states are generated that are best suited to monitor transitions between these ...
Zajkoska Petra P Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, - - 2014
This work demonstrates the first example of the immobilisation of MAO-N whole cells to produce a biocatalyst that remained suitable for repetitive use after 11 months of storage and stable up to 15 months after immobilisation. The production of Escherichia coli expressing recombinant MAO-N was scaled up to bioreactors under regulated, previously ...
Garvican Elaine R - - 2014
Autologous mesenchymal stem cell (MSC) injection into naturally-occurring equine tendon injuries has been shown to be safe and efficacious and protocols inform translation of the technique into humans. Efficient transfer of cells from the laboratory into tissue requires well-validated transport and implantation techniques. Cell viability in a range of media ...
Nagai Yu Y 1 Department of Orthopedic Surgery, Surugadai Nihon University Hospital , Tokyo, Japan - - 2014
Abstract Objective: The purpose of this study was to investigate the effectiveness of photodynamic therapy (PDT) with Na-pheophorbide A in anticancer treatment, using osteosarcoma cells in vitro. Background data: It has been reported that PDT with chlorophyll derivatives inhibits the proliferation of various cancer cells. However, there have been no ...
Jang Wooyoung W Department of Neurology, Hanyang University College of Medicine, Seoul, South - - 2014
The neurotoxicity of levodopa (L-DOPA) on neural stem cells (NSCs) and treatment strategies to protect NSCs from this neurotoxicity remain to be elucidated. Recently, an active form of vitamin D3 has been reported to display neuroprotective properties. Therefore, we investigated the protective effect of 1,25-dyhydroxyvitamin D3 (calcitriol) on L-DOPA-induced NSC ...
Hu Yan-Jian YJ Department of Gastroenterology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. - - 2014
Tumor invasion and metastasis are the main causes of mortality in patients with hepatocellular carcinoma (HCC). Thus, the effective inhibition of these tumorigenic processes is critical in order for HCC therapy to be effective. Previous studies have demonstrated that Notch1 is associated with metastasis in several human malignancies. However, the ...
Guan Tao - - 2014
Overlapping cells segmentation is one of the challenging topics in medical image processing. In this paper, we propose to approximately represent the cell contour as a set of sparse contour points, which can be further partitioned into two parts: the strong contour points and the weak contour points. We consider ...
Luca T T Fondazione Mediterranea "G.B. Morgagni", 95125, Catania, - - 2014
Overexpression or constitutive activation of epidermal growth factor receptors (EGFR) is involved in growth of human cancers. We investigated effects of EGFR and HER-2 blockade in colon cancer cell lines using cetuximab and trastuzumab, with the aim of developing novel approaches to cancer therapy. We studied effects of treatment on ...
Nair Nishanth Ulhas - - 2014
In cell differentiation, a cell of a less specialized type becomes one of a more specialized type,even though all cells have the same genome. Transcription factors and epigenetic marks like histonemodifications can play a significant role in the differentiation process. In this paper, we present a simple analysis of cell ...
Elias Silvia T ST Faculty of Health Sciences, University of Brasília, SQN 205, bloco H apto 201, Asa Norte, Brasilia, DF, 70843-080, - - 2014
Antineoplastic effects of molecules derived from plants have recently gained increasing attention as an additive to traditional therapies. The aim of this study was to evaluate the cytotoxic activity of plant extracts from the Brazilian Cerrado biome associated with radiotherapy in head and neck carcinoma cells (HNSCC). Fifteen extracts derived ...
Buchheit Cassandra L CL 1] Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA. - - 2014
Epithelial cells require attachment to the extracellular matrix (ECM) for survival. However, during tumour progression and metastasis, cancerous epithelial cells must adapt to and survive in the absence of ECM. During the past 20 years, several cellular changes, including anoikis, have been shown to regulate cell viability when cells become ...
da Rosa Marina Rolo Pinheiro MR Department of Oral and Maxillofacial Pathology, School of Dentistry, Federal University of Pará-UFPA, Belém, - - 2014
Ameloblastoma is an odontogenic tumor characterized by local invasiveness and frequent recurrence. The surrounding stroma, composed of different cell types and extracellular matrix (ECM), may influence ameloblastoma invasive behavior. Furthermore, tumor and stromal cells secrete matrix metalloproteases (MMPs), which, in turn, can modulate the matrix and promote the release of ...
Fan Yibo Y Department of Medical Oncology, The First Hospital of China Medical University, No.155, North Nanjing Street, Heping District, Shenyang, 110001, - - 2014
Trypsin is a digestive enzyme that is widely used for cell detachment, which is the first stage of tumor metastasis. Recent studies show that adhesion-related kinases are involved in cell detachment. Proline-rich tyrosine kinase 2 (Pyk2) is a crucial kinase in the regulation of cell adhesion and detachment. However, the ...
Rivas Eva-María EM CEI Campus Moncloa, UCM-UPM, Madrid, Spain; Departamento de Microbiología III. Facultad de Biología, Universidad Complutense de Madrid, C/ José Antonio Nováis, 2, 28040, Madrid, - - 2014
We propose a model, based on the Gompertz equation, to describe the growth of yeasts colonies on agar medium. This model presents several advantages: i) one equation describes the colony growth which previously needed two separate ones (linear increase of radius and of the squared radius); ii) a similar equation ...
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