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Results 201 - 250 of 29278
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Lush Mark E ME Stowers Institute for Medical Research, Kansas City, - - 2014
Damage or destruction of sensory hair cells in the inner ear leads to hearing or balance deficits that can be debilitating, especially in older adults. Unfortunately, the damage is permanent, as regeneration of the inner ear sensory epithelia does not occur in mammals. Zebrafish and other non-mammalian vertebrates have the ...
Li Xiaochen X Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. - - 2014
Transcription factor Krüppel-like factors 5 (KLF5) is overexpressed in a wide range of tumor tissues and acts as a prognostic factor in cancer. However, the role of KLF5 in non-small cell lung cancer is not clear. Hypoxia plays a vital part in the development of cancer via hypoxia-inducible factor 1 (HIF-1). Our ...
Chaudhuri Suhnrita S Department of Laboratory Medicine, School of Tropical Medicine, Cellular and Molecular Immunology Lab, 108, C. R. Avenue, Kolkata, 700073, West Bengal, - - 2014
T-cell-mediated immune responses are typically low in conditions of malignant glioma which has been known to cause marked immunesuppression and dysregulate major T-cell signaling molecules. Thus, T-cell-based immunotherapies are currently in vogue in the treatment of malignant glioma. The novel glycopeptide, T11TS/S-LFA-3/S-CD58 has previously been shown by our group to ...
Hescot Ségolène S Inserm U693, Fac Med Paris Sud, 63 rue Gabriel Péri, 94276, Le Kremlin-Bicêtre Cedex, - - 2014
Mitotane (o,p'DDD) is the most effective treatment of advanced adrenocortical carcinoma (ACC) but its mechanism of action remains unknown. Previous studies suggested that o,p'DDA may represent the active metabolite of mitotane. We aimed at reevaluating the potential role and pharmacological effects of o,p'DDA. Functional consequences of o,p'DDA exposure were studied ...
Chen Jiao - - 2014
Nanomaterial-based photothermal therapy has shown great potential for efficient cancer treatment. Here, we report a new hyperthermia agent, Au-silica nanowire nanohybrid (Au-SiNW nanohybrid) with tunable optical properties, for photothermal therapy. The unique feature of the synthetic method is no need of surface modification of SiNWs for the direct deposition of ...
Abramov Vyacheslav V Institute of Immunological Engineering, Lyubuchany, 142380, - - 2014
Lactobacillus crispatus 2029 isolated upon investigation of vaginal lactobacilli of healthy women of reproductive age was selected as a probiotic candidate. The aim of the present study was elucidation of the role of L. crispatus 2029 in resistance of the female reproductive tract to genitourinary pathogens using cervicovaginal epithelial model. ...
Vicente M Graca Henriques - - 2014
A series of tri[(p-carboranylmethylthio)tetrafluorophenyl]porphyrin conjugates of linear and branched polyamines, glucose, arginine, tri(ethylene glycol), and peptide Tyr-D-Arg-Phe-β-Ala (TAPA) were synthesized. These conjugates were investigated for their BBB permeability in human hCMEC/D3 brain endothelial cells as model, and their cytotoxicity and uptake in human glioma T98G cells. For comparison purposes, a ...
Walczak Elisabeth M EM Cellular and Molecular Biology Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, - - 2014
Wnt/beta-catenin (βcat) signaling is critical for adrenal homeostasis. To elucidate how Wnt/βcat signaling elicits homeostatic maintenance of the adrenal cortex, we characterized the identity of the adrenocortical Wnt-responsive population. We find that Wnt-responsive cells consist of Sonic Hedgehog-producing (Shh) adrenocortical progenitors and differentiated, steroidogenic cells of the zona glomerulosa, but ...
Yang Zhen Z Department of Physics and Texas Center for Superconductivity, University of Houston, Houston, TX 77204-5002;School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, - - 2014
Extraction of intracellular molecules is crucial to the study of cellular signal pathways. Disruption of the cellular membrane remains the established method to release intracellular contents, which inevitably terminates the time course of biological processes. Also, conventional laboratory extractions mostly use bulky materials that ignore the heterogeneity of each cell. ...
Chlon Timothy M TM Cincinnati Children's Hospital Medical Center, Cancer and Blood Diseases Institute, Cincinnati, - - 2014
DNA repair plays a crucial role in embryonic and somatic stem cell biology and cell reprogramming. The Fanconi Anemia pathway, which promotes error-free repair of DNA double strand breaks, is required for somatic cell reprogramming to iPSC. Thus, cells from Fanconi Anemia patients, which lack this critical pathway, fail to ...
Schutte Stacey C SC Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA - - 2014
Endometrial stromal and epithelial cell cross talk is known to influence many of the dynamic changes that occur during the menstrual cycle. We modified our previous model and embedded telomerase-immortalized human endometrial stromal cells and Ishikawa adenocarcinoma epithelial cells in a collagen-Matrigel hydrogel to create a tissue-engineered model of the ...
Gauglhofer Christine C Department of Medicine I, Division: Institute of Cancer Research, Comprehensive Cancer Center, Vienna Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, - - 2014
Recently, we found upregulation of fibroblast growth factor receptor 4 (FGFR4) in a subset of hepatocellular carcinoma (HCC). Here, we provide mechanistic insight into the role of FGFR4-mediated signalling for the aggressive behaviour of HCC cells. To overexpress FGFR4, hepatoma/hepatocarcinoma cells were transfected with a construct coding for FGFR4. For ...
Bhatia Sangeeta N SN Institute for Medical Engineering & Science at MIT, Department of Electrical Engineering and Computer Science, David H. Koch Institute at MIT, and the Howard Hughes Medical Institute, Cambridge, MA 02139, USA. Division of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA. - - 2014
Despite the tremendous hurdles presented by the complexity of the liver's structure and function, advances in liver physiology, stem cell biology and reprogramming, and the engineering of tissues and devices are accelerating the development of cell-based therapies for treating liver disease and liver failure. This State of the Art Review ...
Eastburn Dennis J DJ Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94158, - - 2014
Cell sorting is a central tool in life science research for analyzing cellular heterogeneity or enriching rare cells out of large populations. Although methods like FACS and FISH-FC can characterize and isolate cells from heterogeneous populations, they are limited by their reliance on antibodies, or the requirement to chemically fix ...
Giffin Jennifer L JL Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada N1G - - 2014
The onset of spermatogenesis during prepubertal development is accompanied by dynamic changes in testicular microstructure. Computer-assisted analysis of scrotal ultrasonograms may allow us to track these changes in a noninvasive manner; however, the echotextural characteristics of different histomorphological variables remain unclear. Hence the objective of this study was to compare ...
Kamishibahara Yu Y Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma 374-0193, Japan. Electronic address: - - 2014
Rho kinase (ROCK) is one of the major downstream mediators of Rho. Rho plays crucial regulatory roles in the cellular proliferation and differentiation. Because a ROCK inhibitor, Y-27632, is known to inhibit the dissociation-induced cell death in human embryonic stem (ES) cells, we investigated the effects of this ROCK inhibitor ...
Coelho Ribas João Luiz JL Department of Pharmacology, Federal University of Parana 81531-990, Curitiba-Paraná, - - 2014
The non-steroidal anti-inflammatory drugs are emerging contaminants in aquatic ecosystems. This study aimed to evaluate toxic effects of some representative drugs of this pharmaceutical group on primary culture of monocytic lineage of Hoplias malabaricus anterior kidney. The effects of diclofenac, acetaminophen and ibuprofen in cell viability, lipopolysaccharide (LPS)-induced NO production ...
Kurz David J DJ Center for Molecular Cardiology, Institute of Physiology, University of Zurich, Switzerland; Cardiology, Triemli Hospital, Zurich, Switzerland. Electronic address: - - 2014
Cellular senescence, a programmed state induced by multiple deleterious triggers, is characterised by permanent cell-cycle exit and altered gene expression and cell morphology. In humans it is considered a tumor suppressor mechanism, mediating removal of damaged or mutated cells from the cell-cycle pool, and may also contribute to the ageing ...
Shoshani Ofer O Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA, - - 2014
Over a decade of intensive investigation of the possible plasticity of mammalian cells has eventually substantiated that mammalian species are endowed with a remarkable capacity to change mature cell fates. We review below the evidence for the occurrence of processes such as dedifferentiation and transdifferentiation within mammalian tissues in vivo, ...
Moreau Patrice L PL Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, - - 2014
The viability of Escherichia coli starved of nitrogen (N) or phosphorus (P) decreased by up to 7 orders of magnitude during prolonged incubation under aerobic conditions when exposed to high levels of the antibiotic gentamicin, whereas viability of cells starved of carbon (C) was barely affected. However, the initial rate ...
Sen Nandini N Department of Pediatrics, Stanford University, Stanford, CA 94025, - - 2014
Although pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins. We used this method to investigate varicella-zoster virus (VZV) infection of tonsil T cells, which ...
Astorgues-Xerri Lucile L INSERM U728 and Medical Oncology Department, Beaujon University Hospital (AP-HP - PRES Paris 7 Diderot), 100 bd du Général Leclerc, 92110 Clichy, - - 2014
Galectin-1 (Gal1), a carbohydrate-binding protein is implicated in cancer cell proliferation, invasion and tumour angiogenesis. Several Gal1-targeting compounds have recently emerged. OTX008 is a calixarene derivative designed to bind the Gal1 amphipathic β-sheet conformation. Our study contributes to the current understanding of the role of Gal1 in cancer progression, providing ...
Reyes Juan F JF Neuronal Survival Unit, Department of Experimental Medical Science, Wallenberg Neuroscience Center, Lund University, - - 2014
The transfer of α-synuclein (α-syn) between cells has been proposed to be the primary mechanism of disease spreading in Parkinson's disease. Several cellular models exist that monitor the uptake of recombinant α-syn from the culture medium. Here we established a more physiologically relevant model system in which α-syn is produced ...
Porcù Elena E Department of Woman's and Child's Health, Laboratory of Oncohematology, University of Padova, Via Giustiniani 2, Padova 35128, - - 2014
Tubulin is a major molecular target for anticancer drugs. The dynamic process of microtubule assembly and disassembly can be blocked by various agents that bind to distinct sites on tubulin, usually its β-subunit. Among the antimitotic agents that perturb microtubule dynamics, noscapinoids represent an emerging class of agents. In particular, ...
Nishibuchi Ikuno I Department of Cellular Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan; Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan; Department of Radiation Oncology, Hiroshima Prefectural Hospital, Hiroshima, - - 2014
The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we examined the roles of ...
Henderson D J DJ Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United - - 2014
Malformations affecting the outflow of the heart are a major cause of morbidity and mortality in childhood. While many of these malformations occur sporadically, studies of families with congenital heart defects, alongside animal studies, have revealed that phenotypically discrete heart malformations can have diverse causes, resulting from disruption of a ...
Cacchioli Antonio - - 2014
First evidence of in vitro cytocompatibility of SiC/SiO2 core shell nanowires is reported. Different internalization mechanisms by adenocarcinomic alveolar basal epithelial cells, monocytic cell line derived from an acute monocytic leukemia, breast cancer cells and normal human-derma fibroblasts are shown. The internalization occurs mainly for macropinocytosis and sporadically by direct ...
Zheng Jie - - 2014
Malignant gliomas represent the most common primary brain tumors. The prognosis of patients with malignant gliomas is poor in spite of current intensive therapy and novel therapeutic modalities are needed. Here we report that norcantharidin is effective in growth inhibition of glioma cell lines in vitro. Glioma cell lines (U87 ...
Breit Megan N - - 2014
Exportin 1 (XPO1, also known as CRM1), is a chaperone protein responsible for the export of over 200 target proteins out of the nucleus. The expression and activity of XPO1 is upregulated in several human cancers and its expression is also linked to the development of chemotherapy resistance. Recent studies ...
Miragoli M M Clinical Institute Humanitas IRCCS, Rozzano, - - 2014
Infarct borderzone (BZ) expansion following myocardial infarction is accompanied by ventricular dilatation, remodeling, and heart failure. We showed that myofibroblasts (MFBs), which are present in the BZ, tend to form functional gap junctions with adjacent cardiomyocytes (CMs) in-vitro and such heterocellular pairing depolarizes CMs, induces discontinuity in conduction, and slows ...
Brézillon Stéphane S Laboratoire de Biochimie médicale et de Biologie Moléculaire, UFR de Médecine, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095, Reims Cedex, - - 2014
We recently identified vibrational spectroscopic markers characteristic of standard glycosaminoglycan (GAG) molecules. The aims of the present work were to further this investigation to more complex biological systems and to characterize, via their spectral profiles, cell types with different capacities for GAG synthesis. After recording spectral information from individual GAG ...
La Regina Giuseppe - - 2014
We synthesized 3-aroyl-1-arylpyrrole (ARAP) derivatives as potential anticancer agents having different substituents at the pendant 1-phenyl ring. Both the 1-phenyl ring and 3-(3,4,5-trimethoxyphenyl)carbonyl moieties were mandatory to achieve potent inhibition of tubulin polymerization, binding of colchicine to tubulin and cancer cell growth. ARAP 22 showed strong inhibition of the P-glycoprotein-overexpressing ...
Jin Hyeon-Ok HO KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 139-709, Republic of - - 2014
Piperlongumine (PL) has been shown to selectively induce apoptotic cell death in cancer cells via reactive oxygen species (ROS) accumulation. In this study, we characterized a molecular mechanism for PL-induced cell death. Cell viability and cell death were assessed by MTT assay and Annexin V-FITC/PI staining, respectively. ROS generation was ...
Li Yafeng Y State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou, Fujian 350002, China. - - 2014
Metal-organic frameworks (MOFs) have been used in the area of photovoltaic devices via a novel approach proposed here for the first time. Hierarchical ZnO parallelepipeds were prepared from a MOF precursor, which could then act as an effective light scattering layer in dye-sensitized solar cells, leading to significantly improved cell ...
Sun Xun X State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou, Fujian 350002, China. - - 2014
NiS nanosheets on ITO substrates were synthesized with in situ growth via a one-step hydrothermal route, and exhibited an efficiency of 7.08% which was comparable to the solar cell based on the Pt electrode (7.01%) under the similar conditions.
Heavey Susan S Thoracic Oncology Research Group; Institute of Molecular Medicine; Trinity Centre for Health Sciences; St. James's Hospital; Dublin, - - 2014
Chemoresistance is a major therapeutic challenge to overcome in NSCLC, in order to improve the current survival rates of<15% at 5 years. We and others have shown increased PI3K signaling in NSCLC to be associated with a more aggressive disease, and a poorer prognosis. In this study, targeted inhibition of ...
I┼čeri Ozlem Darcansoy OD Institute of Transplantation and Gene Sciences, Baskent University , Ankara , Turkey - - 2014
Abstract Context: Propranolol, atenolol, and ICI118,551 are non-selective β-adrenergic receptor (AR), β1-AR, and β2-AR antagonists, respectively. Objective: We investigated the efficacy of propranolol, atenolol, and ICI118,551 on proliferation, migration, and invasion of non-stimulated breast (MCF7), colon (HT-29), and hepatocellular (HepG2) cancer cells. Materials and methods: β-AR expression profiling of cells ...
Shokoohinia Yalda Y Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences , Kermanshah , Iran - - 2014
Abstract Context: Prangos ferulacea (L.) Lindl. (Apiaceae) is a perennial plant found in the Middle-East, where it is commonly used as an antispasmodic and anti-inflammatory agent. It is a rich source of coumarins. Objective: To purify several coumarins from P. ferulacea and to screen their cytotoxicity and anti-herpes activity. Materials ...
Hung Chien-Jen CJ Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan ; - - 2014
In this study, we investigated the fine regulation of cell-to-cell movement of Bamboo mosaic virus (BaMV). We report that the coat protein (CP) of BaMV is phosphorylated in planta at position serine 241 (S241), in a process involving Nicotiana benthamiana casein kinase 2α (NbCK2α). BaMV CP and NbCK2α colocalize at ...
Kaur Gurpreet G Department of Environment Studies, Panjab University, Chandigarh, 160014, - - 2014
Plant responses to abiotic stress significantly affect the development of cells, tissues and organs. However, no studies correlating Pb-induced mitotic inhibition and DNA damage and the alterations in redox homeostasis during root division per se were found in the literature. Therefore, an experiment was conducted to evaluate the impact of ...
Li Heping - - 2014
Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancer in developing countries. The prognosis and survival rate of ESCC are very poor. Recently, microRNAs (miRNAs) have emerged as important regulators of cancer cell biological processes. To better understanding the molecular mechanisms by which they regulate the ...
He Juan J College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China. - - 2014
A comprehensive elucidation of the unexpected adverse events that occur in skeletal myoblast transplantation is fundamental for the optimization of myocardial therapeutic effects. However, a well-defined method to study the interactions between skeletal myoblasts and cardiomyocytes during the healing process is out of reach. Here, we describe a microfluidic method ...
Kis M M Department of Medical Physics, University of Szeged, Rerrich Béla tér 1, Szeged, 6720, - - 2014
The development of photosynthetic membranes of intact cells of Rhodobacter sphaeroides was tracked by light-induced absorption spectroscopy and induction and relaxation of the bacteriochlorophyll fluorescence. Changes in membrane structure were induced by three methods: synchronization of cell growth, adjustment of different growth phases and transfer from aerobic to anaerobic conditions ...
Liu Yin - - 2014
Organically modified rectorite (OREC) miro/nanoparticles can be synthesized by organic modification from calcium rectorite (Ca2+-REC or REC), a common form of rectorite in nature. Although OREC has potential applications in food packing and drug delivery, its cytotoxicity is not clear. In the present study, we investigated and compared the cytotoxicity ...
Zheng Hui H Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, - - 2014
Accumulating evidence indicates that the mesenchymal-epithelial transition (MET) and epithelial-mesenchymal transition (EMT) are basic mechanisms for cell fate conversion and may help us understand both physiologic and pathologic processes such as development and carcinogenesis. Here, we further suggest that mammalian cells fall into two grand divisions, mesenchymal or epithelial; interconversions ...
Banfalvi Gaspar G Department of Microbial Biotechnology and Cell Biology, University of Debrecen, 1 Egyetem Square, Debrecen, 4010, Hungary, - - 2014
To visualize characteristic chromatin distortions we have distinguished first among regularly occurring intermediates of chromatin structures in mammalian (Indian muntjac, CHO, murine preB, rat liver, rat myeloid leukemia, K562 human erythroid leukemia) and Drosophila nuclei. Fluorescence microscopy of chromatin structures isolated from nuclei of reversibly permeable cells revealed a common ...
Chen Mei-Hsiu - - 2014
Estradiol plays an important role in the regulation of collagen metabolism. Deficiency of estradiol has been reported to be associated with the degeneration of many connective tissues. However, the association of estradiol and hypertrophy of the ligamentum flavum was seldom explored. Therefore, we studied the effects of estradiol on cultured ...
Ediger Benjamin N BN Department of Pathology and Laboratory Medicine, Children's Hospital of PhiladelphiaDepartment of Medicine and Institute for Diabetes, Obesity and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, - - 2014
Isl-1 is essential for the survival and ensuing differentiation of pancreatic endocrine progenitors. Isl-1 remains expressed in all adult pancreatic endocrine lineages; however, its specific function in the postnatal pancreas is unclear. Here we determine whether Isl-1 plays a distinct role in the postnatal β-cell by performing physiological and morphometric ...
Dermawan Josephine Kam Tai JK Lerner Research Institute/Molecular Genetics, Cleveland - - 2014
Erlotinib is a tyrosine kinase inhibitor approved for the treatment of patients with advanced non-small cell lung cancer (NSCLC). In these patients, erlotinib prolongs survival but its benefit remains modest since many tumors express wild-type (wt) epidermal growth factor receptor (EGFR) or develop a second-site EGFR mutation. To test drug ...
He Ping P Center for Advanced Therapeutic Strategies for Brain Disorders, The Roskamp Institute, Sarasota, FL 34243, - - 2014
Normally, sonic hedgehog (Shh) signaling induces high levels of Patched 1 (Ptc1) and its associated transcription factor Gli1 with genesis of specific neuronal progeny. But their roles in the neural stem cells (NSCs), including glial precursor cells (GPCs), of Alzheimer's disease (AD) are unclear. Here, we show that Ptc1 and ...
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