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Results 201 - 250 of 25374
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Nguyen Trinh Thi My TT Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, - - 2014
Vanillin is derived from lignocellulosic biomass and, as one of the major biomass conversion inhibitors, inhibits yeast growth and fermentation. Vanillin was recently shown to induce the mitochondrial fragmentation and formation of mRNP granules such as processing bodies and stress granules in Saccharomyces cerevisiae. Furfural, another major biomass conversion inhibitor, ...
Hirako Yoshiaki Y Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address: - - 2014
Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of hemidesmosomes was observed when these cells ...
Faye M D MD 1] Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada [2] Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Ottawa, - - 2014
Rhabdomyosarcoma (RMS), a neoplasm characterised by undifferentiated myoblasts, is the most common soft tissue tumour of childhood. Although aggressive treatment of RMS could provide long-term benefit, resistance to current therapies is an ongoing problem. We report here that insulin-like growth factor 2-binding protein 1 (IGF2BP1), an oncofetal protein, is expressed ...
Ding L-W LW Cancer Science Institute of Singapore, National University of Singapore, Singapore, - - 2014
LNK (SH2B3) is an adaptor protein studied extensively in normal and malignant hematopoietic cells. In these cells, it downregulates activated tyrosine kinases at the cell surface resulting in an antiproliferative effect. To date, no studies have examined activities of LNK in solid tumors. In this study, we found by in ...
Chong Eui-Seok ES Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University, 120 Neung-dong-ro, Gwangjin-gu, Seoul 143-701, - - 2014
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying experimental conditions such as the frequency, flow rate, and translational speed ...
Schrems Angelika A Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna, 1190 Austria. - - 2014
We present a rapid and robust technique for the sampling of membrane-associated proteins from the surface of a single, live cell and their subsequent deposition onto a solid-supported lipid bilayer. As a proof of principle, this method has been used to extract green fluorescent protein (EGFP) labelled K-ras proteins located ...
Cheong Soon Keen SK ARC Centre of Excellence in Vision Science, The University of Sydney Eye Hospital Campus, Sydney, New South Wales 2001, - - 2014
We studied the functional connectivity of cells in the lateral geniculate nucleus (LGN) with the primary visual cortex (V1) in anesthetized marmosets (Callithrix jacchus). The LGN sends signals to V1 along parallel visual pathways called parvocellular (P), magnocellular (M), and koniocellular (K). To better understand how these pathways provide inputs ...
Park Kyun Joo KJ Department of Chemical & Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. - - 2014
Single microbial cell encapsulation in hydrogels is an important task to find valuable biological resources for human welfare. The conventional microfluidic designs are mainly targeted only for highly dispersed spherical bioparticles. Advanced structures should be taken into consideration for handling such aggregated and non-spherical microorganisms. Here, to address the challenge, ...
Shaltiel Indra A IA Divisions of Cell Biology and Molecular Oncology, The Netherlands Cancer Institute, 1066 CX, Amsterdam, The - - 2014
The basic machinery that detects DNA damage is the same throughout the cell cycle. Here, we show, in contrast, that reversal of DNA damage responses (DDRs) and recovery are fundamentally different in G1 and G2 phases of the cell cycle. We find that distinct phosphatases are required to counteract the ...
Frasca G G Laboratoire Matière et Systèmes Complexes, CNRS UMR 7057, Université Paris 7, Paris, France. cyprien.gay@univ-paris-diderot.fr - - 2014
In recent decades, significant advances have been made in the description and modelling of tissue morphogenesis. By contrast, the initial steps leading to the formation of a tissue structure, through cell-cell adhesion, have so far been described only for small numbers of interacting cells. Here, through the use of remote ...
Yang Qingyuan Q Department of Clinical Laboratory Medicine, Tenth People's Hospital of Tongji University, Shanghai, China, - - 2014
Neurotrophin receptor-interacting melanoma antigen-encoding gene homolog (NRAGE) is generally recognized as a tumor suppressor as it induces cell apoptosis and suppresses cell metastasis. However, it has recently been reported that NRAGE is overexpressed in lung cancer, melanoma, and colon cancer, implicating a complicated role of NRAGE as we have expected. ...
Gallo Margareth B C MB Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA, - - 2014
As a result of a program to find antitumor compounds of endophytes from medicinal Asteraceae, the steroid (22E,24R)-8,14-epoxyergosta-4,22-diene-3,6-dione (a) and the diterpene aphidicolin (b) were isolated from the filamentous fungi Papulaspora immersa and Nigrospora sphaerica, respectively, and exhibited strong cytotoxicity against HL-60 cells. A proteomic approach was used in an ...
Nilsson Ola O Program in Developmental Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, - - 2014
With age, growth plate cartilage undergoes programmed senescence, eventually causing cessation of bone elongation and epiphyseal fusion. Estrogen accelerates this developmental process. We hypothesized that senescence occurs because progenitor cells in the resting zone are depleted in number and that estrogen acts by accelerating this depletion. To test this hypothesis, ...
Lima Bruno Bezerra BB Departamento de Fisiologia & Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará (UFC), - - 2014
Clostridium difficile toxins A and B (TcdA and TcdB) are homologous glycosyltransferases that inhibit a group of small GTPases within host cells, but several mechanisms underlying their pathogenic activity remain unclear. Here, we evaluated the effects of TcdA on the Wnt/β-catenin pathway, the major driving force behind the proliferation of ...
Lorkowski Martin M Abt. Mikrobiologie, Universität Osnabrück, - - 2014
The invasion of polarized epithelial cells by Salmonella enterica requires the cooperative activity of the Salmonella Pathogenicity Island (SPI)1-encoded type III secretion system (T3SS) and the SPI4-encoded adhesin SiiE. The invasion of polarized cells is more efficient than of non-polarized cells and we observed the formation of clusters of bacteria ...
Sengupta Dipanwita D The Philips Institute of Oral and Craniofacial Molecular - - 2014
Prevotella intermedia is an oral bacterium implicated in a variety of oral diseases. Although internalization of this bacterium by non-phagocytic host cells is well established, the molecular players mediating the process are not well known. Here, the properties of a leucine-rich repeat (LRR) domain protein, designated as AdpF, are described. ...
Hsu Chia-Wei - - 2014
Oral cavity squamous cell carcinoma (OSCC), which is a leading cause of cancer-related death worldwide, is frequently associated with poor prognosis and mortality. The discovery of body fluid-accessible biomarkers may help improve the detection of OSCC. In the present work, we established primary cell cultures derived from OSCC and adjacent ...
Gehring Gerrit G Institute for Molecular Biology, Hannover Medical School, Hannover, - - 2014
Filoviruses such as Ebola virus and Marburg virus cause a severe haemorrhagic fever syndrome in humans for which there is no specific treatment. Since filoviruses use a complex route of cell entry that depends on numerous cellular factors, we hypothesized that there may be drugs already approved for human use ...
Prusevich Polina P Department of Pharmacology, ∥Center for Epigenetics and Department of Medicine, ⊥Department of Ophthalmology, and ¶The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine , Baltimore, Maryland 21205, United - - 2014
Lysine-specific demethylase 1 (LSD1) is an epigenetic enzyme that oxidatively cleaves methyl groups from monomethyl and dimethyl Lys4 of histone H3 (H3K4Me1, H3K4Me2) and can contribute to gene silencing. This study describes the design and synthesis of analogues of a monoamine oxidase antidepressant, phenelzine, and their LSD1 inhibitory properties. A ...
Amir Ariel A Department of Physics and Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, MA - - 2014
Cell walls define a cell's shape in bacteria. The walls are rigid to resist large internal pressures, but remarkably plastic to adapt to a wide range of external forces and geometric constraints. Currently, it is unknown how bacteria maintain their shape. In this paper, we develop experimental and theoretical approaches ...
Ishifune Chieko C Department of Immunology and Parasitology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, - - 2014
The gastrointestinal tract comes into direct contact with environmental agents, including bacteria, viruses, and foods. Intestine-specific subsets of immune cells maintain gut homeostasis by continuously sampling luminal antigens and maintaining immune tolerance. CD11c(+)CX3CR1(+) cells sample luminal antigens in the small intestine and contribute to the trafficking of bacteria to lymph ...
Zhang Hong H State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology (DUT), 2 Linggong Rd., Dalian, 116024, P. R. China. tinglima@dlut.edu.cn - - 2014
The ionic liquid N-butyl-N'-(4-pyridylheptyl)imidazolium bis(trifluoromethane)sulfonimide (BuPyIm-TFSI) was used as a dual-functional additive to improve the electrical properties of the hole-transporting material (HTM) for perovskite solar cells. BuPyIm-TFSI improved the conductivity of HTM and reduced the dark current of the solar cell simultaneously, thereby greatly increasing the power conversion efficiency.
Chevrier Stéphane S Molecular Immunology Division, 2 Bioinformatics Division, 3 Immunology Division, The Walter and Eliza Hall Institute, Parkville, Victoria 3052, - - 2014
The transcriptional network regulating antibody-secreting cell (ASC) differentiation has been extensively studied, but our current understanding is limited. The mechanisms of action of known "master" regulators are still unclear, while the participation of new factors is being revealed. Here, we identify Zbtb20, a Bcl6 homologue, as a novel regulator of ...
Las Guy G Department of Medicine, Boston University School of Medicine, Boston, MA, - - 2014
Mesenchymal stem cells (MSC) are capable of protecting cells harboring mitochondrial damage. This protection is associated with the transfer of mitochondria through tunneling nanotubes (TNT) from MSC to the injured cells. In this issue of The EMBO Journal, the group of Anurag Agrawal shows that mitochondrial transfer is dependent on ...
Yu Lan L From the College of Basic Medical Sciences and Center for Diabetic Systems Medicine, Guilin Medical University, Guilin, - - 2014
The aim of this study was to characterize cells expressing insulin and amylase in adult human pancreas. We applied serial section and immunohistochemistry to pancreas samples from 5 adult nondiabetic subjects (2 men and 3 women; mean age, 65.8 years; random plasma glucose level, 5.1 mM). Cells expressing insulin and ...
Ma Yingxin Y aMed-X Research Institute and School of Biomedical Engineering bInstitute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of - - 2014
CD38 is a multifunctional enzyme that can act as a NADase, generate cyclic adenosine diphosphate-ribose (cADPR) - a key Ca-mobilizing second messenger - and transport cADPR into cells. There have only been a small number of studies on the functions of CD38 in the central nervous system. In this study, ...
Takeuchi Susumu S Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo 113-8603, - - 2014
Pemetrexed (PEM) is currently recommended as one of the standard anticancer drugs for malignant pleural mesothelioma (MPM). However, the mechanism of the sensitivity of MPM to PEM remains unclear. We analyzed the antitumor effects of PEM in six MPM cell lines by MTS assay. To identify genes associated with drug ...
Zhang Chu-Yue CY Department of Obstetrics and Gynecology, International Peace Maternity and Child Health Hospital of China Welfare Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, P.R. - - 2014
Studies have suggested that p16ink4a may be a surrogate biomarker for the diagnosis of cervical cancer; however, the function of p16ink4a in human cervical cancer cells remains largely unknown. Therefore, in this study, we aimed to investi-gate the role of p16ink4a in human cervical cancer cells. Immunocytochemistry was used to ...
Abramova M V Igotti MV Institute of Cytology, Russian Academy of Sciences, St-Petersburg, Russia; St. Petersburg State University, Russia. Electronic address: - - 2014
HDAC inhibitors (HDIs) induce irreversible cell cycle arrest and senescence in E1A+Ras expressing cells. Furthermore, HDIs activate Gadd45a/NF-?B signaling pathway to suppress apoptosis thereby promoting the cell survival. Here, to clarify the role of Gadd45a in realization of the antiapoptotic program, we compared wild-type E1A+Ras cells and the cells with ...
Baumgartner Brett K BK University of Utah Dept. of Human Genetics 15 N. 2030 E. Rm 2100 Salt Lake City, UT - - 2014
Pancreatic exocrine and endocrine lineages arise from multipotent pancreatic progenitor cells (MPCs). Exploiting the mechanisms that govern expansion and differentiation of these cells could enhance efforts to generate β-cells from stem cells. Although our prior work indicates that the canonical Wnt signaling component β-catenin is required qualitatively for exocrine acinar ...
Haeger Anna A Radboud Institute for Molecular Life Sciences, Department of Cell Biology, Post 283, PO Box 9101, 6500HB Nijmegen, The Netherlands. Electronic address: - - 2014
Cancer invasion is a multi-step process which coordinates interactions between tumor cells with mechanotransduction towards the surrounding matrix, resulting in distinct cancer invasion strategies. Defined by context, mesenchymal tumors, including melanoma and fibrosarcoma, develop both single-cell and collective invasion types, however, the mechanical and molecular programs underlying such plasticity of ...
de la Cruz Olga Hernández OH Genomics Sciences Program, Autonomous University of Mexico City, Mexico City, 03100, - - 2014
Actin cytoskeleton is an essential structure involved in cell migration and invasion in parasites. In Entamoeba histolytica, the protozoan parasite causing of human amoebiasis, the mechanisms underlying the expression of migration-related genes are poorly understood. Here, we investigated the biological effects of ectopic overexpression of EhPC4 (positive coactivator 4) in ...
Wang Jing J Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, Institute of Neuroscience, the Second Affiliated Hospital, Soochow University, Suzhou, - - 2014
TAR DNA-binding protein 43 (TDP-43), an essential pathological protein in both amyotrophic later sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), is expressed abnormally in Alzheimer's disease (AD). However, whether and how TDP-43 contributes the pathogenesis of AD remains unknown. We have shown here a colocalization between TDP-43 and the intracellular ...
Ahn Jun-Ho JH Division of Life Sciences, College of Natural Sciences, Incheon National University, Incheon 406-772, Republic of - - 2014
An activating mutation of BRAF (BRAF-V600E) has been reported in a subset of malignant brain tumors. Thus, the aim of the present study was to identify the antiproliferative effect of the new oncogenic B-Raf targeting drug UAI-201 on 6 types of glioma cell lines with differing B-Raf mutational status MAIN ...
Weavers Helen H Department of Zoology, Downing Street, Cambridge CB2 3EJ, - - 2014
The normal development of an organ depends on the coordinated regulation of multiple cell activities. Focusing on tubulogenesis, we review the role of specialised cells or groups of cells that are selected from within tissue primordia and differentiate at the outgrowing tips or leading edge of developing tubules. Tip or ...
Buckley Clare C MRC Centre for Developmental Neurobiology, King's College London, London SE1 1UL, - - 2014
The lumen of the zebrafish neural tube develops precisely at the midline of the solid neural rod primordium. This process depends on cell polarisation and cell rearrangements, both of which are manifest at the midline of the neural rod. The result of this cell polarisation and cell rearrangement is an ...
Buetfering Christina C Department of Clinical Neurobiology at the Medical Faculty of Heidelberg University and German Cancer Research Center (DKFZ), Heidelberg, - - 2014
Grid cells in the medial entorhinal cortex (MEC) generate metric spatial representations. Recent attractor-network models suggest an essential role for GABAergic interneurons in the emergence of the grid-cell firing pattern through recurrent inhibition dependent on grid-cell phase. To test this hypothesis, we studied identified parvalbumin-expressing (PV(+)) interneurons that are the ...
Chen Peipei P Institut National de la Santé et de la Recherche Medicale, U1001; Faculty of Medicine, Paris Descartes University, 75014, Paris, - - 2014
Probing oriented bacterial cell growth on the nanoscale: A novel open-top micro-channel is developed to facilitate the AFM imaging of physically trapped but freely growing bacteria. The growth curves of individual Escherichia coli cells with nanometer resolution and their kinetic nano-mechanical properties are quantitatively measured.
Głowacka Iwona E IE Bioorganic Chemistry Laboratory, Faculty of Pharmacy, Medical University of Łódź, Łódź, - - 2014
A new series of 1-amino-3-(1H-1,2,3-triazol-1-yl)propylphosphonates (R)- and (S)-16 were obtained from enantiomerically pure (R)- and (S)-1-tert-butoxycarbonyl (Boc)-amino-3-azidopropylphosphonates and N-propargylated nucleobases in good yields. All 1,2,3-triazolylphosphonates (R)- and (S)-16 were evaluated for their activities against a broad range of DNA and RNA viruses. Compound (R)-16g (B = 3-acetylindole) was moderately active against vesicular ...
Wang Benjamin L BL 1] Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. - - 2014
Phenotyping single cells based on the products they secrete or consume is a key bottleneck in many biotechnology applications, such as combinatorial metabolic engineering for the overproduction of secreted metabolites. Here we present a flexible high-throughput approach that uses microfluidics to compartmentalize individual cells for growth and analysis in monodisperse ...
Mizokami Atsushi A Department of Integrative Cancer Therapy and Urology, Kanazawa University Graduate School of Medical Sciences 13-1 Takaramachi, Kanazawa, Ishikawa, 920-8640 - - 2014
Androgen blockade-naïve prostate cancer (PCa) develops into CRPC during androgen deprivation therapy (ADT) by various genetic actions. The androgen-AR signaling axis plays a key role in this development. PCa cells mainly adapt themselves to the environment of lower androgen concentrations and change into androgen-hypersensitive cells or androgen-independent cells. Androgens of ...
Zhang Jidong J Department of Biological Science, Graduate School of Science and Technology,. Electronic address: - - 2014
Male gonad development is initiated by the aggregation of pre-Sertoli cells (SCs), which surround germ cells to form cords. Several attempts to reconstruct testes from dissociated testicular cells have been made; however, only very limited morphogenesis beyond seminiferous cord formation has been achieved. Therefore, we aimed to reconstruct seminiferous tubules ...
Bandín Sandra S Department of Cell Biology, Faculty of Biology, University Complutense, 28040 Madrid, - - 2014
Pax6 and Pax7 are transcription factors essential for the development of the CNS. In addition, increasing data, mainly obtained in amniotes, support that they are expressed in subsets of neurons in the adult, likely playing a role in maintaining neuron type identity. In the present study we analyzed the detailed ...
Woo Seung-Hyun SH Howard Hughes Medical Institute, Molecular and Cellular Neuroscience, The Scripps Research Institute, La Jolla, California 92037, - - 2014
How we sense touch remains fundamentally unknown. The Merkel cell-neurite complex is a gentle touch receptor in the skin that mediates slowly adapting responses of Aβ sensory fibres to encode fine details of objects. This mechanoreceptor complex was recognized to have an essential role in sensing gentle touch nearly 50 ...
Maksimovic Srdjan S 1] Department of Dermatology, Columbia University, New York, New York 10032, USA - - 2014
Touch submodalities, such as flutter and pressure, are mediated by somatosensory afferents whose terminal specializations extract tactile features and encode them as action potential trains with unique activity patterns. Whether non-neuronal cells tune touch receptors through active or passive mechanisms is debated. Terminal specializations are thought to function as passive ...
Hai Yanan Y State Key Laboratory of Oncogenes and Related Genes, Stem Cell Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, - - 2014
Spermatogenesis is a complex process by which spermatogonial stem cells (SSCs) self-renew and differentiate into spermatozoa under the elaborate coordination of testicular microenvironment, namely, niche. Sertoli cells, which locate around male germ cells, are the most critical component of the niche. Significant progress has recently been made by peers and ...
Schweigmann H H Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, 35392 Giessen, - - 2014
16α-hydroxy-dehydroepiandrosterone sulfate (16α-OH-DHEAS) mainly originates from the fetus and serves as precursor for placental estriol biosynthesis. For conversion of 16α-OH-DHEAS to estriol several intracellular enzymes are required. However, prior to enzymatic conversion, 16α-OH-DHEAS must enter the cells by carrier mediated transport. To identify these carriers, uptake of 16α-OH-DHEAS by the ...
Fan Rong R Department of Gastroenterology, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin Er Rd, Shanghai, 200025, - - 2014
To detect the expressions of microRNA-218 (miR-218) in an imatinib mesylate-sensitive human gastrointestinal stromal tumor (GIST) cells (GIST882) and an imatinib mesylate-resistant cell line (GIST430) and explore the roles of miR-218 and GIST cells in the sensitivity of gastrointestinal stromal tumor to imatinib mesylate and its potential signaling pathways, with ...
Lammel Tobias T Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Medio Ambiente, Carretera de la Coruña Km 7.5, 28040, Madrid, - - 2014
Graphene and its derivatives are an emerging class of carbon nanomaterial with great potential for a broad range of industrial and consumer applications. However, their increasing production and use is expected to result in release of nano-sized graphene platelets into the environment, where they may interact with chemical pollutants modifying ...
Jantas D D Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12 Street, PL 31-343 Krakow, Poland. Electronic address: - - 2014
Recent studies have documented that metabotropic glutamate receptors from group II and III (mGluR II/III) are a potential target in the symptomatic treatment of Parkinson's disease (PD), however, the neuroprotective effects of particular mGluR II/III subtypes in relation to PD pathology are recognized only partially. In the present study, we ...
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