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Results 201 - 250 of 30333
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Máté Gábor G Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, - - 2014
In this study, the citrinin (CTN)-induced accumulation of reactive oxygen species (ROS) and the regulation of the activities of antioxidant enzymes were investigated in acute toxicity tests in Schizosaccharomyces pombe. 30% of the CTN was accumulated by the cells in 1000 μM CTN solution. In comparison with the control, exposure ...
Unterlauft Jan Darius JD Department of Ophthalmology and Eye Hospital, University of Leipzig, - - 2014
The death of retinal ganglion cells (RGC) leads to visual impairment and blindness in ocular neurodegenerative diseases, primarily in glaucoma and diabetic retinopathy; hence, mechanisms that contribute to protecting RGC from ischemia/hypoxia are of great interest. We here address the role of retinal glial (Müller) cells and of pigment-epithelium-derived factor ...
Franke Jana J Institute of Pharmacology, Pharmacy and Toxicology, University of Leipzig, An den Tierkliniken 15, 04103 Leipzig, - - 2014
Altered airway cell proliferation plays an important role in the pathogenesis of human bronchial asthma and chronic obstructive pulmonary disease (COPD) as well as the equine recurrent airway obstruction (RAO) with consistent changes, i.e. narrowing the airway wall, explained by proliferation and differentiation of fibroblasts. In permanent cell lines, it ...
Lee Do-Hyun DH Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of - - 2014
By virtue of the biocompatibility and physical properties of hydrogel, picoliter-sized hydrogel microcapsules have been considered as a biometric signature that contains several features that are similar to that of the encapsulated single cells, including phenotype, viability, and intracellular content. To maximize the experimental potential of encapsulating cells in hydrogel ...
Chen Yi-Hsin YH Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei, 106, - - 2014
In this study, we compared the detachment ratio of HaCaT and Hs68 cells from pH-responsive chitosan surface by raising medium pH from 7.20 to 7.65 for 60 min. The detachment ratio of elongated Hs68 cells was over 75%, but that of round-shaped HaCaT cells was less than 50%, even extending the incubation time ...
Ni Xi-Lu XL School of Life Science, Northwest University, Xi'an, 710069, China; State Key Laboratory of Seedling Bioengineering, Ningxia Forestry Institute, Yinchuan, 750004, - - 2014
Some species of Allium in Liliaceae have fistular leaves. The fistular lamina of A. fistulosum undergoes a process from solid to hollow during development. The aims were to reveal the process of fistular leaf formation was involved in programmed cell death (PCD), and to compare the cytological events in the ...
Lachambre Simon S CPBS, UMR 5236 CNRS-Université de Montpellier, 1919 Route de Mende, 34293, - - 2014
Cells, especially those of the immune system, can form long and thin connections termed tunneling nanotubes (TNTs). These structures can reach >100 μm in length and, in T-cells, contain actin but no tubulin and are not open ended. T-cell TNTs were found to form following cell contact and to enable ...
Lin Zhaoyu Z Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China; Department of Oral & Maxillofacial Surgery, Sun Yat-sen University, Guangzhou, - - 2014
Epithelial-to-mesenchymal transition (EMT) is implicated in embryonic development and various pathological events. TGFβ has been reported to induce EMT in tumor cells, which is a critical step in the process of metastasis leading to cancer spreading and treatment failure. However, the involvement of microRNA during the EMT process in tongue ...
Zhang Chen - - 2014
PPM1D (protein phosphatase, Mg2+/Mn2+ dependent, 1D) has been reported to be involved in multiple human tumors. This study was designed to investigate the functional role of PPM1D in lung cancer cells. Expression levels of PPM1D were analyzed in A549 and H1299 cells by real-time PCR and Western blotting. Lentivirus-mediated short ...
Chesta María E ME Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), UNC-CONICET, Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 5000, Córdoba, - - 2014
Cultured CAD cells (which lack the microtubule-associated proteins: MAP1B, MAP2, Tau, STOP, and Doublecortin) proliferate in the presence of fetal bovine serum and, in its absence, cease dividing and generate processes similar to the neurites of normal neurons. The reintroduction of serum induces neurite retraction, and proliferation resumes. The neurite ...
Gutiérrez-Iglesias Gisela G Laboratorio de Medicina Regenerativa y Estudios en Cáncer, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, - - 2014
Ceramide is glycosylated to glucosylceramide or lactosylceramide, and this glycosylation is a novel multidrug-resistance (MDR) mechanism. In this work, a short-chain ceramide (C6), lactosylceramide (LacCer), and an inhibitor of ceramide glycosylation (D-threo-1-phenyl-2-decanoylamino-3-1-propanol, PDMP) were evaluated on the proliferation of cervical cancer cells. The participation of glucosylceramide synthase (GCS), P-glycoprotein (P-gp), ...
Wang Han H Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, 77843, - - 2014
Microfluidic live cell arrays with integrated concentration gradient or mixture generators have been utilized in screening cellular responses to various biomolecular cues. Microfluidic network-based gradient generators that can create concentration gradients by repeatedly splitting and mixing different solutions using networks of serpentine channels are commonly used. However, in this method ...
Li Xiao-Ling XL College of Life Sciences and Technology, Jinan University, Guangzhou, China, - - 2014
Human islet amyloid polypeptide (hIAPP) aggregation is linked to loss of pancreatic beta cells in type 2 diabetes, in part due to oxidative stress. Currently, little is known about the effects of selenium-enriched Spirulina on beta cells with the presence of hIAPP. In this study, INS-1E rat insulinoma cells were ...
Zhu Zhitu - - 2014
Single cell transcriptome defined as the entire RNA or polyadenylated products of RNA polymerase II on a cell can describe the gene regulation networks responsible for physiological functions, behaviours, and phenotypes in response to signals and microenvironmental changes. Single cell transcriptome/sequencing has the special power to investigate small groups of ...
Kawamoto Toru T Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Tokyo, - - 2014
Pertuzumab is a humanized monoclonal antibody that binds to HER2 at an epitope that prevents HER2 from dimerizing with ligand-activated HER-family receptors. To assess the potential of pertuzumab as a new therapy, the expression status of HER family members was determined in biliary tract carcinoma (BTC), and the antitumor activity ...
Whipple C A CA Departments of Medicine, Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, and Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, - - 2014
Background:Melanoma, the most lethal form of skin cancer, is responsible for over 80% of all skin cancer deaths and is highly metastatic, readily spreading to the lymph nodes or metastasising to other organs. The frequent genetic mutation found in metastatic melanoma, BRAF(V600E), results in constitutive activation of the mitogen-activated protein ...
Geraghty R J RJ Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, - - 2014
Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and ...
Takahashi Akihisa A a  Advanced Scientific Research Leaders Development Unit, Gunma University, - - 2014
DNA double-strand breaks (DSBs) induced by ionizing radiation pose a major threat to cell survival. The cell can respond to the presence of DSBs through two major repair pathways: homologous recombination (HR) and non-homologous end joining (NHEJ). Higher levels of cell death are induced by high-linear energy transfer (LET) radiation ...
Yamashita Takehiro T Ophthalmology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima, 890-8520, - - 2014
To determine the symmetry of the retinal thicknesses (RT) between the 32 pairs of superior and inferior sectors by posterior pole asymmetrical analysis (PPAA) of the spectral domain optical coherence tomographic (SD-OCT) images in healthy eyes. In addition, to determine their association with the position of the peak retinal nerve ...
Sakimoto Kelsey K KK Department of Chemistry, University of California , Berkeley, California 94720, United - - 2014
Studying bacteria-nanostructure interactions is crucial to gaining controllable interfacing of biotic and abiotic components in advanced biotechnologies. For bioelectrochemical systems, tunable cell-electrode architectures offer a path toward improving performance and discovering emergent properties. As such, Sporomusa ovata cells cultured on vertical silicon nanowire arrays formed filamentous cells and aligned parallel ...
Bi Hui-Chang HC Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China - - 2014
The cell metabolome comprises abundant information that may be predictive of cell functions in response to epigenetic or genetic changes at different stages of cell proliferation and metastasis. An unbiased UPLC-MS-based metabolomics study revealed a significantly altered metabolome for human pancreatic carcinoma PANC-1 cells with gain-of-function noncoding microRNA-1291 (miR-1291), which ...
Dvashi Zeev Z Ophthalmology, Kaplan Medical Center, Rehovot, affiliated with Hadassah-Hebrew University of Jerusalem, Pasternak ST.1, Rehovot, Israel, 76100, - - 2014
PURPOSE. Oxidative stress and cellular senescence are known to contribute to the development of age-related macular degeneration (AMD), however, the mechanism is not fully understood. This study investigated the role of transforming growth factor-β activated kinase 1 (TAK1) in the senescence of retinal pigment epithelial (RPE) cells as a model ...
An Zhenyi Z Center for Autophagy Research; University of Texas Southwestern Medical Center; Dallas, TX USA; Department of Internal Medicine; University of Texas Southwestern Medical Center; Dallas, TX - - 2014
In response to starvation, cells undergo increased levels of autophagy and cell cycle arrest but the role of autophagy in starvation-induced cell cycle arrest is not fully understood. Here we show that autophagy genes regulate cell cycle arrest in the budding yeast Saccharomyces cerevisiae during nitrogen starvation. While exponentially growing ...
Huang Wei W Department of Surgery, and McKusick-Nathans Institute for Genetic Medicine, Johns Hopkins University School of Medicine, 733 N. Broadway, 470 Miller Research Building, Baltimore, MD 21205, - - 2014
As the developing zebrafish pancreas matures, hormone-producing endocrine cells differentiate from pancreatic Notch-responsive cells (PNCs) that reside within the ducts. These new endocrine cells form small clusters known as secondary (2°) islets. We use the formation of 2° islets in the pancreatic tail of the larval zebrafish as a model ...
Ahmed Hany Mohamed Aly HM School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia. Electronic address: - - 2014
The attachment and spreading of mammalian cells on endodontic biomaterials are an area of active research. The purpose of this review is to discuss the cell attachment properties of Portland cement (PC)-based materials by using scanning electron microscope (SEM). In addition, methodological aspects and technical challenges are discussed. A PubMed ...
Xue Rui R Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, - - 2014
A series of cardiac glycosides were isolated and identified from the anti-tumor fraction of root of Streptocaulon juventas in previous studies. In the present research, the cytotoxic activities of the 43 cardiac glycosides on three cell lines, human lung A549 adenocarcinoma cell, large cell lung cancer NCI-H460 cell and normal ...
Stokich Blake B Department of Biological Science, Eastern Illinois University, Charleston, Il - - 2014
A simple method to cryogenically preserve hepatocyte monolayers is currently not available but such a technique would facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. We investigated the effect of trehalose and dimethyl sulfoxide (Me2SO) in cryopreservation of human hepatocellular carcinoma (HepG2) cells ...
Sahin Ba┼čak B Department of Biomedical Engineering, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2V2, - - 2014
Cutaneous T-cell lymphomas (CTCLs) arise from specific molecular aberrations that lead to uncontrolled cell proliferation. RNA interference (RNAi) with short interfering RNAs (siRNAs) is a feasible approach to interrupt aberrant signal processing in CTCL cells, but functional biomaterial carriers are needed to effectively deliver siRNAs intracellularly. Towards this goal, we ...
Filla Mark S MS Department of Ophthalmology & Visual Sciences, Medical Science Center, 1300 University Ave., Madison, WI 53706. Electronic address: - - 2014
In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human ...
Gopalan Vinod V Cancer Molecular Pathology, School of Medicine and Griffith Health Institute, Griffith University, Gold Coast, Queensland, - - 2014
miR-498 is a non-coding RNA located intergenically in 19q13.41. Due to its predicted targeting of several genes involved in control of cellular growth, we examined the expression of miR-498 in colon cancer cell lines and a large cohort of patients with colorectal adenocarcinoma. Two colon cancer cancer cell lines (SW480 ...
Pravin Narayanaperumal N Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India. Electronic address: - - 2014
A series of carboplatin type Cu(II) and Zn(II) metalloinsertors (1-8) having β-diketone analogues and biologically significant cyclobutane-1,1-dicarboxylic acid have been synthesized and characterized by spectral and analytical methods. The binding and cleavage propensity of these metalloinsertors on DNA and their cytotoxic effects in live cells have been explored. From the ...
Manning Alyssa J AJ Department of Biochemistry, Box 357350, The University of Washington, Seattle, WA - - 2014
Epithelia form the building blocks of many tissue and organ types. Epithelial cells often form a contiguous 2-dimensional sheet that is held together by strong adhesions. The mechanical properties conferred by these adhesions allow the cells to undergo dramatic three-dimensional morphogenetic movements while maintaining cell-cell contacts during embryogenesis and post-embryonic ...
Choi Jae Sue JS Department of Food and Life Science, College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of - - 2014
Obesity is a complex, multifactorial, and chronic disease that increases the risk for type 2 diabetes, coronary heart disease and hypertension, and has become a major worldwide health problem. Developing novel anti-obesity drugs from natural products is a promising solution to the global health problem of obesity. While screening anti-obesity ...
Shutova Maria S MS Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, - - 2014
Class II myosins generate contractile forces in cells by polymerizing into bipolar filaments and pulling on anchored actin filaments. Nonmuscle myosin II (NMII) plays central roles during cell adhesion, migration, cytokinesis, and tissue morphogenesis. NMII is present in virtually all mammalian cell types as tissue-specific combinations of NMIIA, NMIIB, and ...
Chowdhury Sayan Mullick SM Department of Biomedical Engineering, Stony Brook University, Stony Brook, - - 2014
We report use of PEG-DSPE coated oxidized graphene nanoribbons (O-GNR-PEG-DSPE) as agent for delivery of anti-tumor drug Lucanthone (Luc) into Glioblastoma Multiformae (GBM) cells targeting base excision repair enzyme APE-1 (Apurinic endonuclease-1). Lucanthone, an endonuclease inhibitor of APE-1, was loaded onto O-GNR-PEG-DSPEs using a simple non-covalent method. We found its ...
Chung Yuan-Chiang - - 2014
In the process of epithelial mesenchymal transition EMT, the disassembly of junctional adhesion complexes such as E-cadherin is a remarkable sign during changes in cell morphology and polarity. However, E-cadherin expression is dynamic, and is regulated by the cellular endocytic system; it is also involved in cell signaling mechanisms. In ...
Zheng Lingling L State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, China. - - 2014
An oligothiophene derivative named DR3TBDTT with high hydrophobicity was synthesized and functioned as the hole transporting material without an ion additive. 8.8% of power conversion efficiency was obtained for CH3NH3PbI3-xClx based planar solar cells with improved stability, compared to devices using Li-TFSI doped spiro-MeOTAD.
Du Yantao Y Zhejiang Provincial Key Laboratory of Pathophysiology, Medical School of Ningbo University, Zhejiang, China; Ningbo Institute of Medical Sciences, Medical School of Ningbo University, Ningbo, Zhejiang, - - 2014
MicroRNAs (miRNAs) are the class of small noncoding RNAs, and play an important role in the regulation of gene expression at the posttranscriptional level. In this study, we explored the effect of miR-129-1-3p on the growth and cell cycle of human gastric cancer cell line BGC-823. The miR-129-1-3p mimics or ...
Calastretti Angela A Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Milan, - - 2014
The critical role of PTEN in regulating the PI3K/Akt/mTOR signaling pathway raises the possibility that targeting downstream effectors of the PI3K pathway, such as Bcl-2, might be an effective anti-proliferative strategy for PTEN-deficient prostate cancer cells. Four prostate cancer cell lines (LNCaP, PC3, DU145, 22Rv1) were assayed for their levels ...
Mi W W Weis Center for Research, Danville, PA, - - 2014
Protein geranylgeranylation (GGylation) is an important biochemical process for many cellular signaling molecules. Previous studies have shown that GGylation is essential for cell survival in many types of cancer. However, the molecular mechanism mediating the cell survival effect remains elusive. In this report, we show that the Hippo pathway mediates ...
Nirogi Ramakrishna R Discovery Research, Suven Life Sciences Limited , Serene Chambers, Banjara Hills, Hyderabad , - - 2014
Abstract Metaphenoxy Benzyl Chloride (3-PBC; CAS # 53874-66-1) is a commonly used intermediate in organic synthesis and is a widely used chemical building block. There is likelihood of human contact with 3-PBC in the process of organic or pesticide synthesis and even through as impurity in the product. Further, this ...
Zhang Linwen L Department of Chemistry, W. M. Keck Institute for Proteomics Technology and Applications, The George Washington University, Washington, DC 20052, USA. - - 2014
Advances in single cell analysis techniques have demonstrated cell-to-cell variability in both homogeneous and heterogeneous cell populations strengthening our understanding of multicellular organisms and individual cell behaviour. However, additional tools are needed for non-targeted metabolic analysis of live single cells in their native environment. Here, we combine capillary microsampling with ...
Nune K C KC Biomaterials and Biomedical Engineering Research Laboratory, Center for Structural and Functional Materials, Institute for Materials Research and Innovation, University of Louisiana at Lafayette, P.O. Box 44130, Lafayette, LA, 70504, - - 2014
The cellular activity, biological response, and consequent integration of scaffold-cell construct in the physiological system are governed by the ability of cells to adhere, proliferate, and biomineralize. In this regard, we combine cellular biology and materials science and engineering to fundamentally elucidate the interplay between cellular activity and interconnected three-dimensional ...
Broustas Constantinos G CG Center for Radiological Research, Columbia University College of Physicians and Surgeons, New York, New - - 2014
Mouse embryonic stem cells null for Rad9 are sensitive to deleterious effects of ionizing radiation exposure. Likewise, integrin β1 is a known radioprotective factor. Previously, we showed that RAD9 downregulation in human prostate cancer cells reduces integrin β1 protein levels and ectopic expression of Mrad9 restores inherent high levels. We ...
Liu Yang Y Key Laboratory of Microsystems and Microstructures Manufacturing, Ministry of Education, Harbin Institute of Technology, Harbin, 150080, China. shaoqinliu@hit.edu.cn - - 2014
A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode ...
Reissmann Siegmund S Friedrich Schiller University, Biological and Pharmaceutical Faculty, Institute of Biochemistry and Biophysics, Dornburger Strasse 25, 07743, Jena, Germany; Jena Bioscience GmbH, Loebstedter Strasse 80, 07749, Jena, - - 2014
The penetration of polar or badly soluble compounds through a cell membrane into live cells requires mechanical support or chemical helpers. Cell-penetrating peptides (CPPs) are very promising chemical helpers. Because of their low cytotoxicity and final degradation to amino acids, they are particularly favored in in vivo studies and for ...
Kim Jaeyun J School of Engineering and Applied Sciences and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, 01238, USA; School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Republic of - - 2014
Three-dimensional macroporous scaffolds have extensively been studied for cell-based tissue engineering but their use is mostly limited to mechanical support for cell adhesion and growth on the surface of macropores. Here, a templated fabrication method is described to prepare cell-friendly inverse opal-like hydrogels (IOHs) allowing both cell encapsulation within the ...
Wang Sz S Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu, 210009, - - 2014
Intervertebral disc degeneration (IDD) is a chronic, complex process associated with low back pain; mechanisms of its occurrence have not yet been fully elucidated. Its process is not only accompanied by morphological changes, but also by systematic changes in its histological and biochemical properties. Many cellular and molecular mechanisms have ...
Iyengar Garud G Industrial Engineering and Operations Research, Columbia University, New York, NY - - 2014
Signaling receptors on the cell surface are mobile and have evolved to efficiently sense and process mechanical or chemical information. We pose the problem of identifying the optimal strategy for placing a collection of distributed and mobile sensors to faithfully estimate a signal that varies in space and time. The ...
Le Anne A Department of Pathology and Oncology,Graduate Program in Pathobiology, dangvchi@exchange.upenn.edu - - 2014
Although aerobic glycolysis provides an advantage in the hypoxic tumor microenvironment, some cancer cells can also respire via oxidative phosphorylation. These respiring ("non-Warburg") cells were previously thought not to play a key role in tumorigenesis and thus fell from favor in the literature. We sought to determine whether subpopulations of ...
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