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Results 201 - 250 of 29388
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Shen Liming L College of Life Science, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060, People's Republic of - - 2014
Nephrogenic systemic fibrosis (NSF) is a fibrosing disorder disease developed in patients with underlying renal insufficiency following exposure to gadolinium-based contrast agents (GBCAs). Previous studies have demonstrated that GdCl3 can promote NIH3T3 fibroblast cell proliferation, which provide a new clue to the role of GBCAs in the development of NSF. ...
Ohashi Haruka H Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu, Tokyo, 183-8509, Japan, - - 2014
Irreversible shifts in ecosystems caused by large herbivores are becoming widespread around the world. We analyzed data derived from the 2009-2010 Sika Deer Impact Survey, which assessed the geographical distribution of deer impacts on vegetation through a questionnaire, on a scale of 5-km grid-cells. Our aim was to identify areas ...
Lee Young Ho YH Division of Rheumatology, Department of Internal Medicine Korea University Anam Hospital, Korea University College of Medicine, 126-1 5 ga, Anam-dong, Seongbuk-gu, Seoul, 136-705, Korea, - - 2014
The aims of this study were to identify candidate single nucleotide polymorphisms (SNPs) and mechanisms of Alzheimer's disease (AD) and to generate SNP to gene to pathway hypotheses. An AD genome-wide association study (GWAS) dataset that included 370,542 SNPs in 1,000 cases and 1,000 controls of European descent was used ...
Jablonska Olga O University of Warmia and Mazury in Olsztyn Department of Zoology Oczapowskiego 1A 10-719 Olsztyn - - 2014
The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a by-product of human industrial activity, was found to affect ovarian steroidogenesis in animals, but the mechanism of its action is still unclear. The aims of the study were to examine the effect of TCDD on (1) progesterone (P4) and oestradiol (E2) production by granulosa cells ...
Ustün Alkan Fulya F Istanbul University Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine Avcilar 34320 Istanbul - - 2014
The present study evaluated the effects of doxorubicin (DOX) and deracoxib (DER), as single agents and in combination treatments, on antioxidant parameters in the canine mammary carcinoma cell line CMT-U27. The cells were exposed to DOX and DER for 24, 48 and 72 h. The viability and malondialdehyde (MDA), nitric ...
Shen Xiao-Fei XF Research Unit of Infection and Immunity, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China; Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, - - 2014
Urinary tract infection (UTI), primarily caused by uropathogenic Escherichia coli (UPEC), is one of the most common infectious diseases worldwide. Emerging antibiotic resistance requires novel treatment strategies. Luteolin, a dietary polyphenolic flavonoid, has been confirmed as a potential antimicrobial agent. Here, we evaluated the sub-MICs of luteolin for potential properties ...
Kim Miri M Department of Conservative Dentistry, Ulsan University, Asan Medical Center, Seoul, - - 2014
OrthoMTA (BioMTA, Seoul, Korea) and Endocem MTA (Maruchi, Wonju-si, Korea) were recently developed to overcome the disadvantages of ProRoot MTA (Dentsply, Tulsa, OK). This study aimed to compare the biological properties of OrthoMTA and Endocem MTA with those of ProRoot MTA using the preosteoblastlike cell line MC3T3-E1. The setting times ...
Marshall Heather D HD Department of Immunobiology, Yale University School of Medicine, New Haven 06520, CT, - - 2014
Regulatory T cells are crucial for preventing autoimmunity, but how their function is restrained to allow optimal effector T cells responses in appropriate contexts is unclear. In a recent paper in the Journal of Experimental Medicine, Campbell and colleagues demonstrate that virus-induced type I interferon acts directly on Treg cells ...
Gurkan-Alp A Selen AS Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Tandogan, 06100, Ankara, Turkey, - - 2014
A series of novel 2-(4-phenoxyphenyl)-1H-benzimidazole derivatives was synthesized and tested in vitro on human chronic myelogenous leukemia (CML) cell line K562. Benzimidazoles containing 5-amidino (10), 5-N-isopropylamidino (11), 5-bromo (13), and 5,6-dimethyl (14) derivatives exhibited remarkable cytotoxic activity. The quantitative analysis of apoptosis by flow-cytometry demonstrated that the percentages of early ...
Mahara Atsushi A a Department of Biomedical Engineering , National Cerebral and Cardiovascular Center Research Institute , Suita , Osaka 565-8565 , - - 2014
We previously developed an antibody-conjugated cell rolling column that successfully separates stem cell subpopulations depending on the cell surface marker density, but a large amount of the injected cells were retained in the column because of non-specific interactions. In this study, an amphiphilic copolymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (nBMA)-co-N-vinyl formamide ...
Rivera Jacqueline F - - 2014
Type 2 diabetes (T2D) is characterized by a deficiency in β cell mass, increased β cell apoptosis, and extracellular accumulation of islet amyloid derived from islet amyloid polypeptide (IAPP), which β cells coexpress with insulin. IAPP expression is increased in the context of insulin resistance, the major risk factor for ...
Zhang Kai - - 2014
Successful single-cell isolation is a primary step for subsequent chemical and biological analyses of single cells. Conventional single-cell isolation methods often encounter operational complexity, limited efficiency, deterioration of cell viability, incompetence in the isolation of single-cell into nanoliter liquid, and/or inability to select single adherent cell with specific phenotype. Here, ...
Kim Jinyoung - - 2014
Islet amyloid accumulation is a hallmark of human type 2 diabetes (T2D). In contrast to human islet amyloid polypeptide (hIAPP), murine islet amyloid polypeptide (mIAPP) does not exhibit amyloidogenic propensity. Because autophagy is important in the clearance of amyloid-like proteins, we studied transgenic mice with β cell-specific expression of hIAPP ...
Rotger Carmen - - 2014
Cell internalization is a major issue in drug design. Although squaramide-based compounds are driving much attention because their interesting bioactivities, cell uptake and trafficking within the cell of this type of compounds are still unknown. In order to monitor the cell internalization process of cycloscuaramide compounds we have prepared two ...
Cao Yang Y Department of Endocrinology, First Affiliated Hospital of Harbin Medical University, No. 24 Youzheng Street, Harbin, 150001, Heilongjiang Province, - - 2014
In recent years, numerous novel anti-diabetic drugs have emerged. Among them, glucagon-like peptide-1 receptor (GLP-1R) agonists developed on the basis of the incretin theory are the most popular and surprising. Thus far, the clinical and experimental efficiency and safety data seem to be good. However, questions about the side effects ...
Zhu Junya J Department of Gastroenterology, Affiliated Hospital of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, - - 2014
SYF2, also known as CCNDBP1-interactor or p29, is reported in pre-mRNA splicing and cell cycle progression. However, the role of SYF2 in esophageal squamous cell carcinoma (ESCC) development remains elusive. In the present study, Western blot and immunohistochemistry assays demonstrated that SYF2 was overexpressed in ESCC tumor tissues and cell ...
Grześkowiak Bartosz F BF Institute of Experimental Oncology and Therapy Research, KLinikum rechts der Isar der Technischen Universität München, Ismaninger Str. 22, 81675, Munich, - - 2014
To explore the potential of magnetofection in delivering pDNA to primary mouse embryonic fibroblasts (PMEFs) and porcine fetal fibroblasts (PFFs) and investigate an effect of magnetic cell labeling on transfection efficacy. The formulation and a dose of the magnetic vector were optimized. The efficacy of the procedure was quantified by ...
Shigihara Nayumi - - 2014
Pancreatic islets in patients with type 2 diabetes mellitus (T2DM) are characterized by loss of β cells and formation of amyloid deposits derived from islet amyloid polypeptide (IAPP). Here we demonstrated that treatment of INS-1 cells with human IAPP (hIAPP) enhances cell death, inhibits cytoproliferation, and increases autophagosome formation. Furthermore, ...
Stupak Robert R a Department of Chemical Technology , Kaunas University of Technology , Kaunas , - - 2014
Disruption of Neisseria denitrificans cells by microfluidizer was optimized using factorial experiments design. pH, pretreatment time, cell concentration, NaCl, EDTA and Triton X-100 concentrations showed significant impact on disruption process and was optimized using central composite design and response-surface methodology (RSM). Investigation revealed optimum conditions: 90 min pretreatment at pH 9.0 ...
Shi Hui H State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, China. kmwang@hnu.edu.cn - - 2014
A novel label-free tailed hairpin-shaped activatable aptamer probe (THAAP) was developed by rationally integrating an aptamer and a split G-quadruplex into one sequence. Based on target recognition-triggered in situ catalysis of split DNAzyme, the THAAP strategy achieved a simple, fast, washing-free, specific and quantitative colorimetric assay of human leukemic CCRF-CEM ...
Tang Yong - - 2014
Akt plays an important role in cell growth, proliferation, and survival. The specific roles of the three Akt isoforms in somatic cell reprogramming have not been investigated. Here we report that during iPSC generation, enhanced Akt1 activity promotes complete reprogramming mainly through increased activation of Stat3 in concert with LIF, ...
Feigal Ellen G EG California Institute for Regenerative Medicine, San Francisco, California, USA; - - 2014
Regenerative medicine is a rapidly evolving field that faces novel scientific and regulatory challenges. In September 2013, the International Workshop on Regulatory Pathways for Cell Therapies was convened to discuss the nature of these challenges and potential solutions and to highlight opportunities for potential convergence between different regulatory bodies that ...
Shin Joongho J Montefiore Medical Center, United - - 2014
The histone deacetylase inhibitor (HDACi) sodium-butyrate promotes differentiation of colon cancer cells as evidenced by induced expression and enzyme activity of the differentiation marker intestinal alkaline phosphatase (ALPi). Screening of a panel of 33 colon cancer cell lines identified cell lines sensitive (42%) and resistant (58%) to butyrate-induction of ALP ...
Hudik Elodie E - - 2014
The majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, ...
Keam Simon P SP Molecular, Structural and Computational Biology Division, Victor Chang Cardiac Research Institute, 405 Liverpool Street, Darlinghurst, NSW, 2010, Australia Faculty of Engineering and Information Technology, Centre of Health Technologies, University of Technology Sydney, 235 Jones Street, Ultimo, NSW, 2007, - - 2014
The Piwi-piRNA pathway is active in animal germ cells where its functions are required for germ cell maintenance and gamete differentiation. Piwi proteins and piRNAs have been detected outside germline tissue in multiple phyla, but activity of the pathway in mammalian somatic cells has been little explored. In particular, Piwi ...
Pryor Sophie E SE Newlife Birth Defects Research Centre, Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, - - 2014
The role of planar cell polarity (PCP) signalling in neural crest (NC) development is unclear. The PCP dependence of NC cell migration has been reported in Xenopus and zebrafish, but NC migration has not been studied in mammalian PCP mutants. Vangl2(Lp/Lp) mouse embryos lack PCP signalling and undergo almost complete ...
McGraw Hillary F HF Oregon Health & Science University, Department of Cell and Developmental Biology, Portland, OR 97239, - - 2014
Canonical Wnt signaling plays crucial roles during development and disease. How Wnt signaling is modulated in different in vivo contexts is currently not well understood. Here, we investigate the modulation of Wnt signaling in the posterior lateral line primordium (pLLP), a cohort of ∼100 cells that collectively migrate along the ...
Jayaprakash Kartheyaene - - 2014
Porphyromonas gingivalis is an important bacterial etiological agent involved in periodontitis. The bacterium expresses two kinds of cysteine proteases called gingipains: arginine gingipains (RgpA/B) and lysine gingipain (Kgp). This study evaluated the interaction between P. gingivalis and THP-1 cells, a widely used monocytic cell line, in vitro with a focus ...
Suram Anitha A Department of Microbiology and Molecular Genetics, NJMS Cancer Center, Rm G1226 Rutgers Biomedical and Health Sciences, 205 South Orange Ave., Newark, NJ, 07103, - - 2014
Telomeres, the ends of our linear chromosomes, can function as 'replicometers', capable of counting cell division cycles as they progressively erode with every round of DNA replication. Once they are critically short, telomeres become dysfunctional and consequently activate a proliferative arrest called replicative senescence. For many years, telomeres were thought ...
Irvin Chaoyu C Department of Medicine, Division of Allergy & Immunology, and Division of Pulmonary and Critical Care Medicine, National Jewish Health, Denver, - - 2014
TH2 cells can further differentiate into dual-positive TH2/TH17 cells. The presence of dual-positive TH2/TH17 cells in the airways and their effect on asthma severity are unknown. We sought to study dual-positive TH2/TH17 cells in bronchoalveolar lavage (BAL) fluid from asthmatic patients, examine their response to glucocorticoids, and define their relevance ...
Currò Monica M Department of Biomedical Sciences and Morphofunctional Imaging, University of Messina, Messina, - - 2014
Recent findings underscore that some natural compounds are responsible for specific biochemical effects, i.e., the activation of redox-sensitive intracellular pathways and modulation of different stress proteins, such as heat shock proteins and sirtuins. Resveratrol, a natural polyphenol widely present in plants, has been shown to display various beneficial effects, including ...
Li Chunyi C State Key Laboratory for Molecular Biology of Special Economic Animals, Changchun, China; Institute of Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China. Electronic address: - - 2014
Deer antler is the only mammalian organ that can fully grow back once lost from its pedicle - the base from which it grows. Therefore, antlers probably offer the most pertinent model for studying organ regeneration in mammals. This paper reviews our current understanding of the mechanisms underlying regeneration of ...
Lee Kwang Bok KB Department of Orthopedic Surgery, Chonbuk National University Medical School and Hospital, Jeonju, 561-181, Republic of - - 2014
The development of esophageal squamous carcinomas (ESC) results from numerous genetic alterations. Our previous study demonstrated that p63 is highly expressed in human ESC cells and stimulates their growth; however, the mechanism by which p63 regulates ESC cell adhesion and invasion remains unclear. In the present study, we further elucidated ...
Tian Xiao-Peng XP State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University, Guangzhou, - - 2014
Paclitaxel is a main ingredient in the combination chemotherapy treatment of advanced human cervical squamous cell carcinomas. We investigated the roles and underlying molecular mechanisms of PinX1 in cervical squamous cell carcinomas (CSCC) cells response to paclitaxel and its clinical significances. The expression dynamics of PinX1 was first examined by ...
Kramann Nadine N Institute of Neuropathology, University Medical Center Göttingen, - - 2014
Subpial cortical demyelination (SCD) accounts for the greatest proportion of demyelinated cortex in multiple sclerosis (MS). SCD is already found in biopsy cases with early MS and in marmosets with experimental autoimmune encephalomyelitis (EAE), but the pathogenesis of SCD is not well understood. The objective of this study was to ...
Jiang Xing-Ran XR Department of Etiology and Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, - - 2014
Human riboflavin transporter 2 (RFT2, also termed as SLC52A3) was recently identified as a susceptibility gene to esophageal squamous cell carcinoma (ESCC), however, its expression and biologic function has remained unclear in ESCC. In this study, we demonstrated that RFT2 was frequently overexpressed in tumor samples compared with normal adjacent ...
Liu Quan Q Department of Neurosurgery, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, P.R. - - 2014
Histone deacetylases (HDACs) constitute a family of enzymes that play important roles in the epigenetic regulation of gene expression and contribute to the growth, differentiation and apoptosis of cancer cells. However, the biological function of HDAC5 in glioma cells has not been fully understood. In the present study, we found that ...
Neufurth Meik M ERC Advanced Investigator Grant Research Group at The Institute for Physiological Chemistry, University Medical Center of The Johannes Gutenberg University Mainz, Duesbergweg 6, D-55128 Mainz, - - 2014
Sodium alginate hydrogel, stabilized with gelatin, is a suitable, biologically inert matrix that can be used for encapsulating and 3D bioprinting of bone-related SaOS-2 cells. However, the cells, embedded in this matrix, remain in a non-proliferating state. Here we show that addition of an overlay onto the bioprinted alginate/gelatine/SaOS-2 cell ...
Thomas Dilip D Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland; Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, - - 2014
Development of cell delivery platforms have been driven based on an empirical cytoprotective design. While cell-matrix and cell-cell interactions that influence biochemical effects beyond survival has been limited and overshadowed in an effort to incrementally improve biomimicking properties of the tissue-engineered constructs. Here we demonstrate fabrication of a shape controlled ...
Gorelik Roman R Laboratoire d'Enzymologie et Biochimie Structurales, Gif-sur-Yvette, - - 2014
The mechanism by which cells control directional persistence during migration is a major question. However, the common index measuring directional persistence, namely the ratio of displacement to trajectory length, is biased, particularly by cell speed. An unbiased method is to calculate direction autocorrelation as a function of time. This function ...
Breier A - - 2014
The development of the most common multidrug resistance (MDR) phenotype is associated with a massive overexpression of P-glycoprotein (P-gp) in neoplastic cells. In the current study, we used three L1210 cell variants: S cells - parental drug-sensitive cells; R cells - drug-resistant cells with P-gp overexpression due to selection with vincristine; T cells - ...
Xia D - - 2014
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin superfamily that has been reported to be involved in a number of neurological and psychological situations. Recently, high expression level of BDNF is observed in diverse human malignancies, delineating a role of BDNF in tumorigenesis. Nevertheless, its effect on B-cell lymphoma remains unclear. ...
Santana Bonilla Alejandro A Institute for Materials Science and Max Bergmann Center of Biomaterials, Dresden University of Technology, 01062 Dresden, Germany. - - 2014
Molecular-based quantum cellular automata (m-QCA), as an extension of quantum-dot QCAs, offer a novel alternative in which binary information can be encoded in the molecular charge configuration of a cell and propagated via nearest-neighbor Coulombic cell-cell interactions. Appropriate functionality of m-QCAs involves a complex relationship between quantum mechanical effects, such ...
Liu Fang F Department of Gastroenterology, China-Japan Friendship Hospital, No.2 Yinghua East Road, Beijing, 100029, People's Republic of - - 2014
Accumulated evidences supported IL-8 play an important role during colorectal carcinogenesis. However, few direct-related evidences are available. In this paper, we found that high level of IL-8 was constitutively present both in familial adenomatous polyposis (FAP) tissue and carcinoma tissue. Using primary epithelial cells from FAP samples, we study IL-8 ...
Li Heng H School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of - - 2014
Comparative studies of immobilized and free cells of Gibberella intermedia CA3-1 in bioconversion of 3-cyanopyridine to nicotinic acid were performed. Entrapping method was chosen based on the advantages in enzymatic activity recovery, mechanical strength and preparation procedure. Four entrapment matrices were investigated and sodium alginate was screened to be the ...
Schmid Lothar L Lehrstuhl für Experimentalphysik I, Soft Matter Group, Universität Augsburg, Universitätsstr. 1, 86159 Augsburg, Germany. - - 2014
We describe a versatile microfluidic fluorescence-activated cell sorter that uses acoustic actuation to sort cells or drops at ultra-high rates. Our acoustic sorter combines the advantages of traditional fluorescence-activated cell (FACS) and droplet sorting (FADS) and is applicable for a multitude of objects. We sort aqueous droplets, at rates as ...
Pérez-Mancera Pedro A PA Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge, CB2 0RE, - - 2014
The core aspect of the senescent phenotype is a stable state of cell cycle arrest. However, this is a disguise that conceals a highly active metabolic cell state with diverse functionality. Both the cell-autonomous and the non-cell-autonomous activities of senescent cells create spatiotemporally dynamic and context-dependent tissue reactions. For example, ...
Cisneros Luis H LH Center for Convergence of Physical Science and Cancer Biology, Arizona State University, Tempe, AZ 85287, - - 2014
We introduce and solve a 'null model' of stochastic metastatic colonization. The model is described by a single parameter θ: the ratio of the rate of cell division to the rate of cell death for a disseminated tumour cell in a given secondary tissue environment. We are primarily interested in ...
Distler Leonie - - 2014
In peritoneal dialysis (PD), glucose degradation products (GDPs), which are formed during heat sterilization of dialysis fluids, lead to structural and functional changes in the peritoneal membrane, which eventually results in the loss of its ultrafiltration capacity. To determine the molecular mechanisms behind these processes, the present study tested the ...
Subrizi Astrid A Centre for Drug Research, Division of Pharmaceutical Biosciences Faculty of Pharmacy, University of Helsinki, Helsinki, Finland, - - 2014
To measure the cytoprotective effects of rhHsp70 against oxidative stress and study its cellular uptake, intracellular and intraocular distribution in the retinal pigment epithelium. Human retinal pigment epithelial cells (ARPE-19) were pre-treated with rhHsp70 for 24 h, 48 h, and 72 h before being exposed to 1.25 mM hydrogen peroxide. Non-treated cells served as ...
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