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Cerca Filipe F ICBAS-UP - Instituto de Ciências Biomédicas de Abel Salazar - Universidade do - - 2014
Staphylococcus epidermidis is the most commonly isolated etiological agent of nosocomial infections mainly due to its ability to establish biofilms on indwelling medical devices. Detachment of bacteria from S. epidermidis biofilms and subsequent growth in the planktonic form is a hallmark in the pathogenesis of these infections leading to dissemination. ...
Benner Christopher - - 2014
Insulin producing beta cell and glucagon producing alpha cells are colocalized in pancreatic islets in an arrangement that facilitates the coordinated release of the two principal hormones that regulate glucose homeostasis and prevent both hypoglycemia and diabetes. However, this intricate organization has also complicated the determination of the cellular source(s) ...
Edmunds Lia R LR Children's Hospital of Pittsburgh of UPMC, United - - 2014
myc-/- rat fibroblasts (KO cells) differ from myc+/+ (WT) cells and KO cells with enforced Myc re-expression (KO-Myc cells) with respect to mitochondrial structure and function, utilization of glucose and glutamine as energy-generating substrates, and ATP levels. Specifically, KO cells demonstrate low levels of glycolysis and oxidative phosphorylation, dysfunctional mitochondria ...
Doughty Michael J MJ Department of Vision Sciences, Glasgow Caledonian University, Glasgow, - - 2014
To objectively assess the morphology of superficial conjunctival surface cells showing squamous metaplasia as seen in contact lens wearers. Impression cytology samples were taken from interpalpebral bulbar conjunctiva from 23 young adult white European women with an average of 6 years of successful daily soft contact lens wear and 23 ...
Nakajima Takeshi T Senju Laboratory of Ocular Sciences, Kobe, SENJU Pharmaceutical Co., Ltd., 1-5-5, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, - - 2014
Purpose: Activation of calpains (calpain 2 and Lp82) in rodent lenses readily causes proteolysis and cataract formation. In contrast, primate lenses are quite resistant to activation of calpains. The hypothesis is that high levels of human endogenous calpain inhibitor, calpastatin (CS), prevent calpain activation in human lenses. The purpose of ...
Wu Daoming D College of Resources and Environment, South China Agricultural University, Guangzhou 510642, - - 2014
Al-induced cell rigidity is one of the symptoms of Al toxicity, but the mechanism by which plants tolerate this toxicity is still unclear. In this study, we found that overexpression of OsPIN2, an auxin transporter gene, could alleviate Al-induced cell rigidity in rice root apices. A freeze-thawing experiment showed that ...
Shamloo Amir A Center of Excellence in Energy Conversion (CEEC), School of Mechanical Engineering, Sharif University of Technology, P.O. Box: 11155-9567, Tehran, - - 2014
This study investigates the role of cell-cell and cell-ligand interactions in cytoskeleton organization of endothelial cells and their directional migration within a microfluidic device. The migration of endothelial cells in response to a biochemical factor was studied. Mathematical analysis of the cell migration pathways and cellular cytoskeleton revealed that directional ...
Ou Xijun X Immunology Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore - - 2014
The adaptor Downstream-of-Kinase (DOK) 3 functions as a negative regulator and attenuates B-cell receptor-mediated calcium signaling. Although DOK3 is dispensable for early B-cell development, its role in plasma cell (PC) differentiation is unknown. Here, we show that Dok3(-/-) mice have increased populations of T follicular-helper (Tfh) and germinal center (GC) ...
Shemesh Jonathan J Department of Biomedical Engineering andRussell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 32000, - - 2014
Microfluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be applied for single-cell analysis; however, to investigate encapsulated cells effectively over prolonged time periods, an array of droplets must remain stationary on a versatile substrate for optimal cell compatibility. We present here a platform of unique geometry and ...
Lee Juyeon J School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea; Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, - - 2014
Elmo is an evolutionarily conserved mammalian ortholog of C. elegans CED-12 with proposed roles during removal of apoptotic cells, cell migration, neurite outgrowth, and myoblast fusion [1-4]. Elmo mediates these cellular processes by interacting with various proteins located in the plasma membrane, cytoplasm and nucleus, and by modulating their activities ...
Haupaix Nicolas N CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer, Observatoire Océanologique, 06230, Villefranche-sur-mer, France; Sorbonne Universités, UPMC Univ Paris 06, Laboratoire de Biologie du Développement de Villefranche-sur-mer, Observatoire Océanologique, 06230, Villefranche-sur-mer, France; Université de Nice Sophia Antipolis, Nice, - - 2014
Recent evidence suggests that ascidian pigment cells are related to neural crest-derived melanocytes of vertebrates. Using live-imaging, we determine a revised cell lineage of the pigment cells in Ciona intestinalis embryos. The neural precursors undergo successive rounds of anterior-posterior (A-P) oriented cell divisions, starting at the blastula 64-cell stage. A ...
Plum Jakob J Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, - - 2014
The GABA-mimetic anti-epileptic drug substance vigabatrin is used against infantile spasms. In vitro and in vivo experiments have shown that vigabatrin is transported via the proton coupled amino acid transporter (PAT1) mediating at least parts of the intestinal absorption of the drug. However, such evidence does not preclude the involvement ...
Hartwig Christina C Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address: - - 2014
Adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) are well-established second messengers, whereas the physiological role of the cyclic pyrimidine nucleotides cytidine 3',5'-cyclic monophosphate (cCMP) and uridine 3',5'-cyclic monophosphate (cUMP) is poorly understood. Certain mammalian nucleotidyl cyclases (NCs) and bacterial NC toxins can generate cCMP and cUMP. Human HEK293 ...
Seol Hyang Sook HS Department of Pathologym University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, South Korea; Asan Center for Cancer Genome Discovery, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, South - - 2014
The role of microRNAs (miRNAs) in carcinogenesis as tumor suppressors or oncogenes has been widely reported. Epigenetic change is one of the mechanisms of transcriptional silencing of miRNAs in cancer. To identify lung cancer-related miRNAs that are mediated by histone modification, we conducted microarray analysis in the Calu-6 non-small cell ...
Watanabe Tadashi T Department of Cell Biology, Kyoto Pharmaceutical University, Misasagi-Shichonocho 1, Yamashinaku, Kyoto 607-8412, - - 2014
Primary effusion lymphoma (PEL) is a subtype of non-Hodgkin's B-cell lymphoma and is an aggressive neoplasm caused by Kaposi's sarcoma-associated herpesvirus (KSHV) in immunosuppressed patients. In general, PEL cells are derived from post-germinal center B-cells and are infected with KSHV. To evaluate potential novel anti-tumor compounds against KSHV-associated PEL, seven ...
de Winter Lenneke L Bioprocess Engineering, AlgaePARC, Wageningen University, w***ww.algaePARC.com, PO Box 8129, 6700 EV, Wageningen, The Netherlands; Wetsus - Center of Excellence for Sustainable Water Technology, PO Box 1113, 8900 CC Leeuwarden, The Netherlands. Electronic address: - - 2014
The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian clock. The results were compared to ...
Song Yuxian Y The State Key Laboratory of Pharmaceutical Biotechnology; Division of Immunology; Medical School; Nanjing University; Nanjing, PR - - 2014
MicroRNAs (miRNAs) dysregulation is critically involved in lung cancer. Regulating miRNAs by natural agents may be a new strategy for cancer treatment. We previously found that a novel small-molecule compound diaporine A (D261), a natural product of endophytic fungus 3lp-10, had potential anti-cancer activites. In the present study, the inhibitory ...
Barazzuol L - - 2014
Abstract Purpose: Prognosis for patients with glioblastoma (GBM) remains poor, and new treatments are needed. Here we use a combination of two novel treatment modalities, carbon ions and a histone deacetylase inhibitor (HDACi). We compare these to conventional X-rays, measuring the increased effectiveness of carbon ions as well as radiosensitisation ...
Tsai Meng-Lin - - 2014
We realized photovoltaic operation in large-scale MoS2 monolayers by the formation of a type-II heterojunction with p-Si. The MoS2 monolayer introduces a built-in electric field near the interface between MoS2 and p-Si to help photo-generated carrier separation. Such a heterojunction photovoltaic device achieves a power conversion efficiency of 5.23%, which ...
Singh Ajay Vikram - - 2014
This study reports a facile biomineralization route to gold microplates (GMPs) synthesis using bovine serum albumin (BSA) as reductant and stabilizing agent. Adding BSA to HAuCl4 solution yields spontaneous versatile anisotropic and partially hollow GMPs upon ageing. We hypothesize that, the instantaneous protein denaturation at low pH enabled access to ...
Kolacsek Orsolya O Research Centre for Natural Sciences, HAS, Institute of Molecular Pharmacology, Budapest, Hungary ; - - 2014
The Sleeping Beauty (SB) and piggyBac (PB) DNA transposons represent an emerging new gene delivery technology, potentially suitable for human gene therapy applications. Previous studies pointed to important differences between these transposon systems, depending on the cell types examined and the methodologies applied. However, efficiencies cannot always be compared due ...
Wang W-T WT Molecular Oncology Unit, YCR/CRUK Sheffield Cancer Research Centre, Department of Oncology, University of Sheffield School of Medicine, Sheffield, - - 2014
While DNA damage response pathways are well characterized in cancer cells, much less is known about their status in normal cells. These pathways protect tumour cells from DNA damage and replication stress and consequently present potential therapeutic targets. Here we characterize the response of human telomerase reverse transcriptase (hTERT)-immortalized normal ...
Reinke Emily N - - 2014
ABSTRACT The treatment of chronic myelogenous leukemia with specific tyrosine kinase inhibitors typically results in clinical success although therapeutic failure frequently occurs. In order to investigate the biological consequences of treating CML cells with such drugs, we previously reported that the anti-oxidant selenoprotein GPx-1 was induced by imatinib in both ...
Kanjilal Baishali B U.S. Army Research Institute of Chemical Defense, Pharmacology and Immunology, Gunpowder, United - - 2014
Abstract The US military has placed major emphasis on developing therapeutics against nerve agents. Current efforts are hindered by the lack of effective in vitro cellular models to aid in the preliminary screening of potential candidate drugs/antidotes. The development of an in vitro cellular model to aid in discovering new nerve agent ...
Zhang Xiu-Zhen XZ 1 School of Life Sciences, Shandong University of Technology , Zibo, China - - 2014
Abstract This study aims at evaluating the anticancer effects of berberine hydrochloride (berberine) and d-limonene, alone and in combination, on human gastric carcinoma cell line MGC803 to determine whether berberine and d-limonene work synergistically and elucidate their mechanisms. MGC803 cells were treated with berberine and d-limonene, alone and in combination, ...
García-Gómez Candela C Department of Ecology, Faculty of Sciences, University of Málaga, Bvd. Louis Pasteur s/n, 29010 Málaga, Spain. - - 2014
The effects of increased CO2 and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA + UVB) irradiances, simulating the solar radiation at different depths, at present (390 ppmv, LC) and predicted CO2 levels for the year 2100 (1000 ppmv, HC). ...
MacKay Joanna L JL Department of Chemical and Biomolecular Engineering, University of California-Berkeley, Berkeley, California 94720, - - 2014
The GTPases RhoA and Rac1 are key regulators of cell spreading, adhesion, and migration, and they exert distinct effects on the actin cytoskeleton. While RhoA classically stimulates stress fiber assembly and contraction, Rac1 promotes branched actin polymerization and membrane protrusion. These competing influences are reinforced by antagonistic crosstalk between RhoA ...
Hartwig Johannes J Institute for Cell Biology, Center for Molecular Biology of Inflammation, Münster University, D-48149 Münster, - - 2014
The control over the acquisition of cell motility is central for a variety of biological processes in development, homeostasis, and disease. An attractive in vivo model for investigating the regulation of migration initiation is that of primordial germ cells (PGCs) in zebrafish embryos. In this study, we show that, following ...
Bubendorfer Sebastian S Max Planck Institute for Terrestrial Microbiology and LOEWE Research Center for Synthetic Microbiology (SYNMICRO), 35043 Marburg, Germany;Institute for Microbiology and Molecular Biology, IFZ Interdisciplinary Research Centre, Justus Liebig University Giessen, 35392 Giessen, Germany;Institute for Medical Microbiology and Hospital Epidemiology, Hannover Medical School, 30625 Hannover, Germany; - - 2014
As numerous bacterial species, Shewanella putrefaciens CN-32 possesses a complete secondary flagellar system. A significant subpopulation of CN-32 cells induces expression of the secondary system under planktonic conditions, resulting in formation of one, sometimes two, filaments at lateral positions in addition to the primary polar flagellum. Mutant analysis revealed that ...
Rodriguez-Villalon Antia A Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne, - - 2014
The phloem performs essential systemic functions in tracheophytes, yet little is known about its molecular genetic specification. Here we show that application of the peptide ligand CLAVATA3/EMBRYO SURROUNDING REGION 45 (CLE45) specifically inhibits specification of protophloem in Arabidopsis roots by locking the sieve element precursor cell in its preceding developmental ...
Liu Zhiqiang Z Department of Lymphoma and Myeloma, Division of Cancer Medicine, the University of Texas MD Anderson Cancer Center, Houston, TX, United - - 2014
Hedgehog (Hh) signaling plays an important role in the oncogenesis of B-cell malignancies such as multiple myeloma (MM). However, the source of Hh ligand sonic hedgehog (SHH) and its target cells remain controversial. Previous studies showed that stromally-induced Hh signaling was essential for the tumor cells and CD19(+)CD138(-) MM stem ...
Fatfat Maamoun - - 2014
Metals including iron, copper and zinc are essential for physiological processes yet can be toxic at high concentrations. However the role of these metals in the progression of cancer is not well defined. Here we study the anti-tumor activity of the metal chelator, TPEN, and defined its mechanism of action. ...
Lee Chi-Chiu CC Department of Food Science and Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 202, Taiwan, - - 2014
This study reports on a cytotoxic toxin derived from the venom of the crown-of-thorns starfish Acanthaster planci (CAV). The protein toxin was isolated through both ion-exchange and gel-filtration chromatography, and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrum analyzes. The CAV was identified as plancitoxin I protein. ...
Zhang Pei-Pei PP Tianjin Medical University, - - 2014
MicroRNAs (miRNAs) have been shown to play important roles in carcinogenesis. However, their underlying mechanisms of action in hepatocellular carcinoma (HCC) are poorly understood. Recent evidence suggests that epigenetic silencing of miRNAs through tumor suppression by CpG island hypermethylation may be a common hallmark of human tumors. Here, we demonstrated ...
Götze Silke S Max Planck Institute of Molecular Physiology, Dortmund, - - 2014
Histone deacetylase inhibitors (HDIs) specifically affect cancer cells by inducing cell cycle arrest, activate apoptotic pathways and re-activate epigenetically silenced tumor suppressor genes, but their pleiotropic mode of action is not fully understood. Despite the clinical effects of HDIs in the treatment of hematological malignancies, their potency against solid tumors ...
Choi So Ra SR Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, - - 2014
Cutaneous squamous cell carcinoma (SCC) is a very common resectable cancer; however, cutaneous SCC is highly resistant to chemotherapy if metastasis develops. Activating transcription factor 3 (ATF3) has been suggested as a marker of advanced or metastatic cutaneous SCC. Autophagy is one of the most important mechanisms in cancer biology ...
Korea Charalampia G CG Novartis Vaccines and Diagnostics, Siena, - - 2014
The opportunistic pathogen Staphylococcus aureus is one of the major causes of healthcare-associated infections. S. aureus is primarily an extracellular pathogen, but recently it has been reported to invade and replicate in several host cell types. The ability of S. aureus to persist within cells has been implicated in resistance ...
Liu Bin B Laboratory of Hepatobiliary Surgery, Guangdong Medical College, Zhanjiang Key Laboratory of Hepatobiliary Diseases, Zhanjiang, Guangdong 524001, P.R. - - 2014
The present study compared the effect of resveratrol on HCT116 and Caco‑2 human colon cancer cells. Annexin V/propidium iodide staining, MTT assay and western blot analysis revealed that resveratrol induced cycle arrest in the two cell lines, which was evidenced by cell cycle analysis and changes in the expression of the ...
Konya Christine C Division of Rheumatology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA *Christine Konya and Ziv Paz contributed equally to the writing of this - - 2014
To describe our current understanding of the role of T cells in the pathophysiology of systemic lupus erythematosus (SLE). Over the last few years, the dominant role of T cells in autoimmunity and SLE was established. Genome-wide-association studies led to the discovery of multiple single-nucleotide polymorphisms associated with SLE. Most ...
Huang Meng M Department of Pharmacy, Faculty of Science, National University of Singapore, Singapore 117543, Republic of - - 2014
Meisoindigo has been a routine therapeutic agent in the clinical treatment of chronic myelogenous leukemia (CML) in China since the 1980s. In the present study, the in vitro antileukemic activity of meisoindigo was investigated in acute promyelocytic leukemia (APL) cells, acute myeloid leukemia (AML) cells, and myelomonocytic leukemia cells (NB4, NB4.007/6, HL-60 and ...
Li Xin-Xiang XX Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. - - 2014
The CXC chemokine receptor 7 (CXCR7) has been reported to be involved in cell growth, metastasis and apoptosis in certain cancers. However, the function and molecular mechanisms of CXCR7 in human colorectal cancer (CRC) are still undefined. In the present study, sixty-eight cases of CRC tissues and corresponding adjacent non-cancer ...
Li Hongyu H Department of Gastroenterology, General Hospital of Shenyang Military Area, Shenyang, Liaoning 110840, P.R. - - 2014
The aim of the present study was to investigate the effects of PTEN and KAI1 gene overexpression on the proliferation, metastasis and radiosensitivity of ASPC‑1 pancreatic cancer cells under hypoxic conditions. Recombinant vectors that overexpress PTEN and KAI1 genes were transfected into hypoxic ASPC‑1 cells. Protein expression levels were detected ...
Meniche Xavier X Department of Microbiology and Physiological Systems - - 2014
Mycobacteria are surrounded by a complex multilayered envelope and elongate at the poles. The principles that organize the coordinated addition of chemically diverse cell wall layers during polar extension remain unclear. We show that enzymes mediating the terminal cytosolic steps of peptidoglycan, arabinogalactan, and mycolic acid synthesis colocalize at sites ...
Ghaffari Hadi H Department of Studies in Biotechnology, University of Mysore, Mysore, Karnataka, - - 2014
Oxidative stress plays a key role in several ailments including neurodegenerative conditions. The aim of the study was to demonstrate the effect of rosmarinic acid (RA) in preventing oxidative stress related death of neuronal cell lines. In the present study, we demonstrated direct neuroprotective effect of RA using H2O2-induced oxidative ...
McLane Joshua S JS Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY. 12180-3590, USA; Department of Biology, Rensselaer Polytechnic Institute, Troy, NY. 12180-3590, - - 2014
The stromal tissue surrounding most carcinomas is comprised of an extracellular matrix densely packed with collagen-I fibers, which are often highly aligned in metastatic disease. Here we developed an in vitro model to test the effect of an aligned fibrous environment on cancer cell morphology and behavior, independent of collagen ...
Sun Jing J Department of Periodontology, School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, 44-1# West Wenhua Road, Jinan, 250012, Shandong, People's Republic of - - 2014
Periodontal ligament-associated protein-1 (PLAP-1) is a newly discovered member of the extracellular matrix family of proteins known as proteoglycans and is a negative regulator that plays a crucial role in the homeostasis of periodontal tissues. It can protect the periodontal ligament from excessive osteogenesis. However, the molecular mechanisms of PLAP-1 ...
Sehrawat Anuradha A Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA, - - 2014
We showed previously that zerumbone (ZER), a sesquiterpene isolated from subtropical ginger, inhibited in vitro (MCF-7 and MDA-MB-231cells) and in vivo (MDA-MB-231 cells) growth of human breast cancer cells in association with apoptosis induction. Here, we investigated the role of Notch receptors in anticancer effects of ZER (cell migration inhibition ...
Li Bo B Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, - - 2014
Clear cell renal cell carcinoma (ccRCC), the most common form of kidney cancer, is characterized by elevated glycogen levels and fat deposition. These consistent metabolic alterations are associated with normoxic stabilization of hypoxia-inducible factors (HIFs) secondary to von Hippel-Lindau (VHL) mutations that occur in over 90% of ccRCC tumours. However, ...
Tang Qin Q 1] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. - - 2014
Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) ...
Zhang Ming M University of Toronto, - - 2014
Glucagon-like peptide-1 (GLP-1) is an incretin hormone that regulates glucose homeostasis. Due to their direct stimulation of insulin secretion from pancreatic beta cells, GLP-1 receptor (GLP-1R) agonists are now important therapeutic options for the treatment of type 2 diabetes. To better understand the mechanisms that control the insulinotropic actions of ...
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