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Völker Timo T Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35043 Marburg - - 2014
The catalysis of bioorthogonal transformations inside living organisms is a formidable challenge-yet bears great potential for future applications in chemical biology and medicinal chemistry. We herein disclose highly active organometallic ruthenium complexes for bioorthogonal catalysis under biologically relevant conditions and inside living cells. The catalysts uncage allyl carbamate protected amines ...
Lee Young-Hoon YH School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, - - 2014
We have previously shown that microRNAs (miRNAs) miR- 760, miR-186, miR-337-3p, and miR-216b stimulate premature senescence through protein kinase CK2 (CK2) downregulation in human colon cancer cells. Here, we examined whether these four miRNAs are involved in the replicative senescence of human lung fibroblast IMR-90 cells. miR-760 and miR-186 were ...
Liu Shunli S Department of Burn and Plastic, General Hospital of Jinan Military Region, 25 Shifan Road, Jinan, 250031, - - 2014
Cancer stem cells (CSCs) in most human tumors are commonly identified and enriched using similar strategies for identifying normal stem cells, including flow cytometry assays for side population, high aldehyde dehydrogenase (ALDH) activity, and CD133 positivity. Thus, development of a method for isolating a specific cancer using cancer-specific characteristic appears ...
Ding Zhongyang Z Department of Surgery, Nanjing Medical University affiliated Wuxi People's Hospital, Wuxi, 214023, - - 2014
Akt2 overexpression correlates with chemoresistance of colorectal cancer (CRC). However, the cellular functions and precise signals elicited by Akt2 in LSCC have not been elucidated. Here, we transfected a CRC cell line HCT116 with Akt-2 targeted shRNA in order to establish a cell line with Akt2 knockdown. In vitro experiments ...
Hocine Nora - - 2014
Abstract Purpose: To improve risk assessments associated with chronic exposure to Strontium-90 (Sr-90), for both the environment and human health, it is necessary to know the energy distribution in specific cells or tissue. Monte Carlo (MC) simulations codes are extremely useful tools for calculating deposition energy. The present work is ...
Sweeney Sinbad S Lung Cell Biology, Section of Pharmacology and Toxicology, National Heart and Lung Institute , Imperial College London, London , UK - - 2014
Abstract There can be significant variability between bioreactivity studies of nanomaterials that are apparently the same, possibly reflecting differences in the models used and differing sources of experimental material. In this study, we have generated two crystal forms of titanium dioxide nanoparticles (nano-TiO2), pure anatase and pure rutile to address ...
Ha Tae Kwang TK Department of Biological Sciences, KAIST, 335 Gwahak-ro, Yuseong-gu, Daejeon, 305-701, South Korea, - - 2014
Lithium chloride (LiCl), which is a specific inhibitor of glycogen synthase kinase-3β, is known to induce cell cycle arrest at the G2/M phase and to regulate apoptosis. To determine the potential of LiCl as a chemical additive to enhance specific productivity (q p) of recombinant Chinese hamster ovary (rCHO) cells ...
Bian Xiao-Jie XJ Department of Urology, Fudan University Shanghai Cancer Center, No. 270, Dong'an Road, Shanghai, 200032, - - 2014
Dicer and Argonaute2 (Ago2) are critical components responsible not only for RNA interference but also for microRNA synthesis. The present study investigated the roles of Dicer and Ago2 in prostate cancer (Pca). First, the expression levels of Dicer and Ago2 in Pca tissues were determined by immunohistochemistry (IHC) and compared ...
He Chunbai - - 2014
Real-time measurement of intracellular pH in live cells is of great importance for understanding physiological/pathological processes and developing intracellular drug delivery systems. We report here the first use of nanoscale metal-organic frameworks (NMOFs) for intracellular pH sensing in live cells. Fluorescein isothiocyanate (FITC) was covalently conjugated to a UiO NMOF ...
Sattlegger Evelyn E Institute of Natural and Mathematical Sciences, Massey University, Albany, New - - 2014
Classic functions of the actin cytoskeleton include control of cell size and shape and the internal organization of cells. These functions are manifest in cellular processes of fundamental importance throughout biology such as the generation of cell polarity, cell migration, cell adhesion, and cell division. However, studies in the unicellular ...
Johnson Abby L AL University of - - 2014
Vitamin D3 receptor (VDR) signaling within the mammary gland regulates various postnatal stages of glandular development including puberty, pregnancy, involution, and tumorigenesis. Previous studies have shown that vitamin D3 treatment induces cell autonomous growth inhibition and differentiation of mammary epithelial cells in culture. Further, mammary adipose tissue serves as a ...
Charwat Verena V BioSensor Technologies, AIT Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. - - 2014
We have developed a lab-on-a-chip system for continuous and non-invasive monitoring of microfluidic cell cultures using integrated high-frequency contactless impedance spectroscopy. Electrically insulated microfabricated interdigitated electrode structures were embedded into four individually addressable microchambers to reliably and reproducibly detect cell-substrate interactions, cell viability and metabolic activity. While silicon nitride passivated ...
Saito Mikako M Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan, - - 2014
The concentration-dependent effect of spermine was investigated on the spermine-induced generation of multilayer myotube sheets (MMTS) from mouse embryoid bodies (EBs). During spermine treatment for 24 h, a monolayer cell sheet that had already grown radially from the periphery of an EB was exfoliated. The exfoliation was inhibited by z-VAD.fmk, indicating ...
Oakes Patrick W PW Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, University of Chicago, Chicago, - - 2014
Cells generate mechanical stresses via the action of myosin motors on the actin cytoskeleton. Although the molecular origin of force generation is well understood, we currently lack an understanding of the regulation of force transmission at cellular length scales. Here, using 3T3 fibroblasts, we experimentally decouple the effects of substrate ...
Wang Shunqiang S Department of Mechanical Engineering & Mechanics, Lehigh University, Bethlehem, PA 18015, USA. - - 2014
While substrates with nanopillars (NPs) have emerged as promising platforms for isolation of circulating tumor cells (CTCs), the influence of diameter and spacing of NPs on CTC capture is still unclear. In this paper, CTC-capture yield and cell behaviors have been investigated by using antibody functionalized NPs of various diameters ...
Diekmann Ulf U Institute of Clinical Biochemistry, Hannover Medical School , Carl-Neuberg-Str.1 , Hannover, Lower Saxony, Germany , 30625 , +495115322836 , +495115323584 ; - - 2014
Differentiation of pluripotent cells into endoderm related cell types initially requires in vitro gastrulation into the definitive endoderm. Most differentiation protocols are initiated from colonies of pluripotent cells complicating their adaption due to insufficiently defined starting conditions. The protocol described here was initiated from a defined cell number of dispersed ...
Alex Jimi M JM Laboratory for Drug Design and Synthesis, Centre for Chemical and Pharmaceutical Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, - - 2014
A series of 17 analogs of 1-acetyl-4,5-dihydro(1H)pyrazoles (JP-1 to JP-17) bearing two aromatic rings at positions 3 and 5, either of which ought to be heterocyclic, were synthesized and evaluated for their anti-proliferative potential against breast cancer (MCF-7 and T-47D) and lung cancer (H-460 and A-549) cell lines for the ...
Haque I I 1] Cancer Research Unit, V.A. Medical Center, Kansas City, MO, USA [2] Division of Hematology and Oncology, Department of Medicine, University of Kansas Medical Center, Kansas City, MO, - - 2014
The matricellular protein CCN5/WISP-2 represents a promising target in triple-negative breast cancer (TNBC) because treatment or induced activation of CCN5 in TNBC cells promotes cell growth arrest at the G0/G1 phase, reduces cell proliferation and delays tumor growth in the xenograft model. Our studies found that the p27(Kip1) tumor suppressor ...
Korir Michelle L ML Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, - - 2014
Group B Streptococcus (GBS) causes severe disease in neonates, the elderly and immunocompromised individuals. GBS is highly diverse and can be classified by serotype and multilocus sequence typing. Sequence type (ST)-17 strains more frequently cause invasive neonatal disease. Attachment and invasion of host cells is a key step in GBS ...
Bonde Sara S Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, - - 2014
The endeavor of exploiting arrays of vertical one-dimensional (1D) nanostructures (NSs) for cellular applications has recently been experiencing a pronounced surge of activity. The interest is rooted in the intrinsic properties of high-aspect-ratio NSs. With a height comparable to a mammalian cell, and a diameter 100-1000 times smaller, NSs should ...
Thomas A A IBR West Extension, School of Cancer Sciences, College of Medicine and Dentistry, University of Birmingham, Birmingham, - - 2014
Topoisomerase inhibitors are in common use as chemotherapeutic agents although they can display reduced efficacy in chemotherapy-resistant tumours, which have inactivated DNA damage response (DDR) genes, such as ATM and TP53. Here, we characterise the cellular response to the dual-acting agent, Alchemix (ALX), which is a modified anthraquinone that functions ...
García-Ocaña Adolfo - - 2014
Adult human pancreatic β cells are refractory to current therapeutic approaches to enhance proliferation. This reluctance to expand is problematic, especially for people with diabetes who lack sufficient numbers of functional insulin-producing β cells and could therefore benefit from therapies for β cell expansion. In this issue of the JCI, ...
Brand Toni M TM Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, - - 2014
The EGFR antibody cetuximab is used to treat numerous cancers, but intrinsic and acquired resistance to this agent is a common clinical outcome. In this study, we show that overexpression of the oncogenic receptor tyrosine kinase AXL is sufficient to mediate acquired resistance to cetuximab in models of non-small cell ...
Alzoubi Kousi K Department of Physiology, University of Tübingen, Tuebingen, - - 2014
Sulforaphane, an isothiocyanate from cruciferous vegetable, counteracts malignancy. The effect is at least in part due to stimulation of suicidal death or apoptosis of tumour cells. Mechanisms invoked in sulforaphane-induced apoptosis include mitochondrial depolarization and altered gene expression. Even though lacking mitochondria and nuclei, erythrocytes may, similar to apoptosis of ...
Moirangthem Dinesh Singh - - 2014
Cephalotaxus spp. are known to possess anticancer potential. In this present work, for the first time the effects of C. griffithii needle (CGN) extracts on human cancer cells were examined. The CGN was successively extracted with petroleum ether (PE), acetone and methanol. The extracts were tested for its effect on ...
Tadokoro Tomomi T Department of Cell Biology, Duke University Medical Center, Durham, NC 27710; - - 2014
The pseudostratified airway epithelium of the lung contains a balanced proportion of multiciliated and secretory luminal cells that are maintained and regenerated by a population of basal stem cells. However, little is known about how these processes are modulated in vivo, and about the potential role of cytokine signaling between ...
Orticello M M Institute of Biology, Molecular Medicine and Nanobiotechnology, CNR National Research Council, Rome, - - 2014
Mitotic proteins are attractive targets to develop molecular cancer therapeutics due to the intimate interdependence between cell proliferation and mitosis. In this work, we have explored the therapeutic potential of the kinetochore (KT) protein Hec1 (Highly Expressed in Cancer protein 1) as a molecular target to produce massive chromosome missegregation ...
Edens Lance E - - 2014
Polymers and oligomers with conjugated phenylene ethynylene or thiophene ethynylene backbones have been shown to be potent antimicrobials. The mechanisms by which they act have been unclear, though AFM imaging of Escherichia coli cells before and after exposure to two such biocides, PPE-Th polymer and EO-OPE-1(C3), show their effects on ...
Han Rong R College of Pharmaceutical Science, Soochow University, Suzhou 215123, - - 2014
Aim:Crotoxin (CrTX) is the primary toxin in South American rattlesnake (Crotalus durissus terrificus) venom, and exhibits antitumor and other pharmacological actions in vivo and in vitro. Here, we investigated the molecular mechanisms of the antitumor action of CrTX in human lung carcinoma cells in vitro.Methods:Human lung squamous carcinoma SK-MES-1 cells ...
Pastukhov Oleksandr O Institute of Pharmacology, University of Bern, Friedbühlstrasse 49, CH-3011 Bern, - - 2014
Ceramide kinase (CerK) catalyzes the generation of ceramide-1-phosphate (C1P) which may regulate various cellular functions, including inflammatory reactions and cell growth. Here, we studied the effect of a recently developed CerK inhibitor, NVP-231, on cancer cell proliferation and viability, and investigated the role of cell cycle regulators implicated in these ...
Sarmento L M LM Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, - - 2014
Checkpoint kinase 1 (CHK1) is a key component of the ATR (ataxia telangiectasia-mutated and Rad3-related)-dependent DNA damage response pathway that protect cells from replication stress, a cell intrinsic phenomenon enhanced by oncogenic transformation. Here, we show that CHK1 is overexpressed and hyperactivated in T-cell acute lymphoblastic leukemia (T-ALL). CHEK1 mRNA ...
Atkinson Samantha S School of Biological Sciences, Illinois State University, Normal, IL, 61790, - - 2014
Aligned microtubule arrays spatially organize cell division, trafficking, and determine the direction of cell expansion in plant cells. In response to changes in environmental and developmental signals, cells reorganize their microtubule arrays into new configurations. Here, we tested the role of microtubule nucleation during hormone-induced microtubule array reorientation. We have ...
Gabilondo Hugo H Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049, Madrid, - - 2014
Studies in the Drosophila embryonic NB4-2 lineage have suggested that the transcription factor Klumpfuss (Klu) functions within embryonic neuroblast lineages to differentiate between the identities of two adjacent ganglion mother cells (GMCs). However, because of the limited lineage markers available, these observations have been made only for the NB4-2 lineage. ...
Wong Ian Y IY 1] BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA - - 2014
During cancer progression, malignant cells in the tumour invade surrounding tissues. This transformation of adherent cells to a motile phenotype has been associated with the epithelial-mesenchymal transition (EMT). Here, we show that EMT-activated cells migrate through micropillar arrays as a collectively advancing front that scatters individual cells. Individual cells with ...
Liu Xiaofang X Stem Cell and Regenerative Medicine Laboratory, Beijing Institute of Transfusion Medicine, Beijing, 100850, - - 2014
Cell-cell contacts and interactions between pancreatic β-cells and/or other cell populations within islets are essential for cell survival, insulin secretion, and functional synchronization. Three-dimensional (3D) culture systems supply the ideal microenvironment for islet-like cluster formation and functional maintenance. However, the underlying mechanisms remain unclear. In this study, mouse insulinoma 6 ...
Cheung Kevin J KJ Department of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University, 855 N. Wolfe St, 452 Rangos Bldg, Baltimore, MD 21205, USA; Department of Oncology, School of Medicine, Johns Hopkins University, 855 N. Wolfe St, 452 Rangos Bldg, Baltimore, MD 21205, USA. Electronic address: - - 2014
Metastasis begins when tumors invade into surrounding tissues. In breast cancer, the study of cell interactions has provided fundamental insights into this complex process. Powerful intravital and 3D organoid culture systems have emerged that enable biologists to model the complexity of cell interactions during cancer invasion in real-time. Recent studies ...
Plácido Ana I AI Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga; Faculty of Medicine, Pólo I, 3004-504, Coimbra, - - 2014
Cerebral amyloid angiopathy resulting from the deposition of misfolded amyloid beta (Aβ) peptide in the walls of brain's blood vessels is exhibited by the majority of Alzheimer's disease (AD) patients, suggesting that alterations in protein quality control contribute to AD-associated vascular dysfunction. The present work addressed the role of ER ...
Horiguchi Kotaro K Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, 476 Miyashita-cho, Hachioji, Tokyo, 192-8508, Japan, - - 2014
S100β-positive cells, which do not express the classical pituitary hormones, appear to possess multifunctional properties and are assumed to be heterogeneous in the anterior pituitary gland. The presence of several protein markers has shown that S100β-positive cells are composed of populations such as stem/progenitor cells, epithelial cells, astrocytes and dendritic ...
Fan Jian J Department of Laboratory Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, Zhejiang Province, 310003, - - 2014
Histone deacetylases (HDACs) play a critical role in the proliferation, differentiation, and apoptosis of cancer cells. An obstacle for the application of HDAC inhibitors as effective anti-cancer therapeutics is that our current knowledge on the contributions of different HDACs in various cancer types remains scarce. The present study reported that ...
Aureli Massimo M Department of Medical Biotechnology and Translational Medicine, University of Milano, Via Fratelli Cervi 93, 20090, Segrate, - - 2014
The aim of radiotherapy is to eradicate cancer cells with ionizing radiation; tumor cell death following irradiation can be induced by several signaling pathways, most of which are triggered as a consequence of DNA damage, the primary and major relevant cell response to radiation. Several lines of evidence demonstrated that ...
Fribley Andrew M AM Carmen and Ann Adams Department of Pediatrics, Children's Hospital of Michigan, Detroit, MI 48201; Molecular Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI 48201; Wayne State University School of Medicine. Electronic address: - - 2014
The survival rate for patients with oral squamous cell carcinoma (OSCC) has not seen marked improvement in recent decades despite enhanced efforts in prevention and the introduction of novel therapies. We have reported that pharmacological exacerbation of the unfolded protein response (UPR) is an effective approach to killing OSCC cells. ...
Ma Shuwei S Pharmaceutical Engineering, Institute of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, PR China. Electronic address: - - 2014
In this report, the transport of ginkgolides with different lipophilicities was investigated using an hCMEC/D3 cell monolayer as a blood-brain barrier (BBB) cell model in vitro in an attempt to explain ginkgolide transport path mediated by lipophilicity. The log P values of ginkgolides were determined by measuring the distribution of ...
Nakajima Yuki Y Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, - - 2014
A series of experiments was conducted to investigate migration, proliferation and differentiation of gonadal germ cells (GGCs) collected from the gonads of 7-day-old chick embryos under cross-sex germline chimera conditions. The migratory and proliferative abilities of exogenous GGCs were examined by transferring 50 fluorescently labeled GGCs collected from White Leghorn ...
Wouters An - - 2014
Regions within solid tumours often experience oxygen deprivation, which is associated with resistance to chemotherapy and irradiation. The aim of this study was to evaluate the radiosensitising effect of gemcitabine and its main metabolite dFdU under normoxia versus hypoxia and to determine whether hypoxia-inducible factor 1 (HIF-1) is involved in ...
Li Jipeng - - 2014
MicroRNAs (miRNAs) have been identified as important posttranscriptional regulators involved in various biological and pathological processes of cells, but their association with tumor chemoresistance has not been fully understood. We detected miR-27a expression in two lung adenocarcinoma cell lines, A549 and A549/CDDP, and then investigated the effects of miR-27a on ...
Zhai Ronglin R Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, - - 2014
We aimed to study the post-translational regulation of CD151 by the microRNA miR-152. CD151 is highly expressed in gastric cancer (GC) and has been shown to accelerate GC by enhancing invasion and metastasis; however, its regulation is still unclear. Our results showed decreased expression of miR-152 in GC tissue samples ...
Ninomiya Hiromasa H Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1, Komaba, Meguro-ku, Tokyo, 153-8902, - - 2014
Improvement of methods to produce endoderm-derived cells from pluripotent stem cells is important to realize high-efficient induction of endodermal tissues such as pancreas and hepatocyte. Difficulties hampering such efforts include the low efficiency of definitive endoderm cell induction and establishing appropriate defined culture conditions to ensure a safe cell source ...
Pfisterer Karin K Molecular Immunology Unit, Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna A-1090, - - 2014
The spatial and temporal organization of T cell signaling molecules is increasingly accepted as a crucial step in controlling T cell activation. CD222, also known as the cation-independent mannose 6-phosphate/insulin-like growth factor 2 receptor, is the central component of endosomal transport pathways. In this study, we show that CD222 is ...
Zhang Wenjie - - 2014
Saponins of several herbs are known to induce apoptosis in many cancer cells. The present study aimed to investigate the growth inhibitory effect of Paris saponin VII (PS VII), a kind of steroidal saponins from Chonglou (Rhizoma Paridis Chonglou), on the human cervical cancer cell line Hela and the relative ...
Xu Bo B Organic Chemistry, Center of Molecular Devices, Department of Chemistry, School of Chemical Science and Engineering, KTH Royal Institute of Technology, 10044, Stockholm, - - 2014
Two carbazole-based small molecule hole-transport materials (HTMs) are synthesized and investigated in solid-state dye-sensitized solar cells (ssDSCs) and perovskite solar cells (PSCs). The HTM X51-based devices exhibit high power conversion efficiencies (PCEs) of 6.0% and 9.8% in ssDSCs and PSCs, respectively. These results are superior or comparable to those of ...
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