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Nascimento Emmani B M EB Department of Human Biology, Maastricht University, P.O. Box 616; 6200MD Maastricht, the - - 2014
ASC-1, PAT2, and P2RX5 are newly identified cell-surface proteins that may distinguish brown/beige from white adipocytes in mouse and human adipose tissue (Ussar et al., this issue).
Xu Ping P Bristol-Myers Squibb, 519Route173West, Bloomsbury, NJ, - - 2014
A unique and nontraditional approach using glutamine and asparagine supplements for CHO-GS cell lines was studied. In our experiments, we found that a decrease in pH and an increase in cell death occurred in production phase of a GS cell line, leading to reduced antibody expression and lower antibody yields. ...
Marusyk Andriy A 1] Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215, USA [2] Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA [3] Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, - - 2014
Cancers arise through a process of somatic evolution that can result in substantial sub-clonal heterogeneity within tumours. The mechanisms responsible for the coexistence of distinct sub-clones and the biological consequences of this coexistence remain poorly understood. Here we used a mouse xenograft model to investigate the impact of sub-clonal heterogeneity ...
Dominguez Natalia N Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, - - 2014
Chromogranins (Cgs) are acidic proteins that have been described in the large, dense core vesicles (LDCVs) of adrenal chromaffin cells and that have been shown to promote LDCV formation, even in nonsecretory cells. Catecholamines (CAs) are adsorbed by Cgs in vitro, and the absence of Cgs modifies the storage and ...
Severa Godwin G Hawaii Natural Energy Institute, University of Hawaii, Honolulu, Hawaii, - - 2014
This work evaluates the ability of an ionic liquid-methanol co-solvent system to extract lipids and recycle fermentable sugars recovered from oil-bearing Rhodosporidium toruloides grown in batch culture on defined media using glucose and xylose as carbon sources. Growth on the recycled mixed carbon substrate was successful with glucose consumed before ...
Lohr Verena - - 2014
In human vaccine manufacturing some pathogens such as Modified Vaccinia Virus Ankara, measles, mumps virus as well as influenza viruses are still produced on primary material derived from embryonated chicken eggs. Processes depending on primary cell culture, however, are difficult to adapt to modern vaccine production. Therefore, we derived previously ...
Peng Lei L School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P.R. - - 2014
Amyloid-β peptides such as Aβ1-42 (Aβ42) play a pivotal role in the progression of Alzheimer's disease (AD). Aβ42 is neurotoxic and can activate microglial cells. These cells in turn migrate toward senile (neuritic) plaques and help to clear Aβ deposits through an endocytotic mechanism. It is of potential significance to ...
Lu Yi-Ching YC Department of Physiology and Pharmacology, Institute of Biomedical Sciences, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kuei-Shan, Tao-Yuan 33302, Taiwan, Republic of China. - - 2014
Nanoparticles may serve as carriers in targeted therapeutics; interaction of the nanoparticles with a biological system may determine their targeting effects and therapeutic efficacy. Epigallocatechin-3-gallate (EGCG), a major component of tea catechins, has been conjugated with nanoparticles and tested as an anticancer agent. We investigated whether EGCG may enhance nanoparticle ...
Jiang Wenting - - 2014
The poor permeability of glioma parenchyma represents a major hurdle for anti-glioblastoma drug delivery. In this study, gracilaria lemaneiformis polysaccharid (GLP), which has a high binding affinity to αvβ3 integrin overexpressed in glioma cells, was employed to functionalize selenium nanoparticles (SeNPs) to achieve anti-glioblastoma efficacy. GLP-SeNPs showed satisfactory size distribution, ...
Golson Maria L ML Tennessee Valley Healthcare System Department of Veteran Affairs, Nashville, - - 2014
The forkhead box transcription factor FoxM1, a positive regulator of the cell cycle, is required for β-cell mass expansion postnatally, during pregnancy, and after partial pancreatectomy. Upregulation of full-length FoxM1, however, is unable to stimulate increases in β-cell mass in unstressed mice or after partial pancreatectomy, likely due to lack ...
Hosokawa Masahito - - 2014
A straightforward in vivo monitoring technique for biomolecules would be an advantageous approach for understanding their spatiotemporal dynamics in living cells. However, the lack of adequate probes has hampered the quantitative determination of the chemical composition and metabolomics of cellular lipids at single-cell resolution. Here, we describe a method for ...
Qiu Yongcai - - 2014
Nitrogen-doped graphene (NG) is a promising conductive matrix material for fabricating high performance Li/S batteries. Here we report a simple, low-cost, and scalable method to prepare an additive-free nanocomposite cathode in which sulfur nanoparticles are wrapped inside the NG sheets (S@NG). We show that the Li/S@NG can deliver high specific ...
Hamm Rebecca R Institute of Pharmacy and Biochemistry, Department of Pharmaceutical Biology, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, - - 2014
Resistance of cancer cells towards chemotherapy is the major cause of therapy failure. Hence, the evaluation of cellular defense mechanisms is essential in the establishment of new chemotherapeutics. Archazolid B, a novel vacuolar H(+)-ATPase inhibitor, displayed cytotoxicity in the low nanomolar range on a panel of different tumor cell lines. ...
Zhao Ziqi - - 2014
3D multicellular spheroids (MCSs) mimic the structure and function of real tissue much better than the conventional 2D cell monolayers, however, their application was severely hindered by difficulties in their generation. An ideal method for MCS fabrication should produce spheroids with narrow size distribution and allow for control over their ...
Feldman Steven A SA National Cancer Institute, Surgery Branch , 10 Center Drive, MSC 1201 , Bldg. 10, ACRF, 1B37A , Bethesda, Maryland, United States , 20892 , 3014964269 , 3014804968 ; - - 2014
Efforts to improve the biosafety of γ-retroviral mediated gene therapy have resulted in a shift towards the use of self-inactivating (SIN) γ-retroviral vectors. However, scale-up and manufacturing of such vectors requires significant optimization of transient transfection-based processes or development of novel platforms for the generation of stable producer cell clones. ...
Chiu M M Department of Biomedical Biotechnological and Translational Sciences (SBiBiT), University of Parma, Parma, - - 2014
Background:A subset of human hepatocellular carcinomas (HCC) exhibit mutations of β-catenin gene CTNNB1 and overexpress Glutamine synthetase (GS). The CTNNB1-mutated HCC cell line HepG2 is sensitive to glutamine starvation induced in vitro with the antileukemic drug Crisantaspase and the GS inhibitor methionine-L-sulfoximine (MSO).Methods:Immunodeficient mice with subcutaneous xenografts of the CTNNB1-mutated ...
Wong Hui Chyn HC Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, - - 2014
3,19-(3-Chloro-4-fluorobenzylidene)andrographolide (SRJ23), a new semisynthetic derivative of andrographolide (AGP), exhibited selectivity against prostate cancer cells in the US National Cancer Institute (NCI) in vitro anti-cancer screen. Herein, we report the in vitro growth inhibition and mechanisms of cell cycle arrest and apoptosis induced by SRJ23. 3-(4,5-Dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay ...
So Eui Young - - 2014
BRAT1 (BRCA1-associated ATM activator 1) interacts with both BRCA1, ATM and DNA-PKcs, and has been implicated in DNA damage responses. However, based on our previous results, it has been shown that BRAT1 may be involved in cell growth and apoptosis, besides DNA damage responses, implying that there are undiscovered functions ...
Sun Yang Y Division of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, One Blackfan Circle, 6th Floor, Boston, MA, 02115, - - 2014
Lymphangioleiomyomatosis (LAM) is a female-predominant lung disease that can lead to respiratory failure. LAM cells typically have inactivating tuberous sclerosis 2 (TSC2) mutations, leading to mTORC1 hyperactivation. The gender specificity of LAM suggests that female hormones contribute to disease progression. Clinical findings indicate that estradiol exacerbates LAM behaviors and symptoms. ...
Shah Mansi K MK College of Pharmacy and Health Sciences, St. John's University , Queens, NY , - - 2014
Abstract Enhanced oral bioavailability of poorly aqueous soluble drugs encapsulated in solid lipid nanoparticles (SLNs) via lymphatic delivery has been documented. Since no in-vitro lymphoid tissue is currently available, human excised Caco-2 cell monolayer could be alternative tissue for development of an in-vitro model to be used as a screening ...
Cheng Xin-Bing XB Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, PR - - 2014
Entrapment of free lithium in a 3D fibrous Li7 B6 framework allows LiB nanostructured anodes with stable interfaces between the electrolyte and the electrode, which retards the formation of lithium dendrites. A lithium-sulfur cell with a nanostructured anode is created with a high Coulombic efficiency and high capacity retention rate ...
Baldini Enke E E Baldini, Department of Experimental Medicine, "Sapienza" University of Rome, Rome, - - 2014
Aurora kinases are serine/threonine kinases that play an essential role in cell division. Their aberrant expression and/or function induce severe mitotic abnormalities resulting in either cell death or aneuploidy. Aurora kinases are often found overexpressed in several malignancies among which the anaplastic thyroid carcinoma (ATC). We previously demonstrated the in ...
Hawkins Kate K Stem Cell Research Group, The University of Manchester, Core Technology Facility, 46 Grafton Street, Manchester, M13 9NT, - - 2014
Mouse embryonic stem (mES) cells and epiblast stem cells (EpiSCs) represent the naïve and primed pluripotent states respectively. These cells self-renew via distinct signalling pathways and can transition between the two states in the presence of appropriate growth factors. Manipulation of signalling pathways has therefore allowed the isolation of novel ...
Miyai Tomohiro T Laboratory for Immune Regeneration,Graduate School of Frontier Biosciences, Osaka University, Yamada-oka, Suita, Osaka 565-0871, - - 2014
The immune system is influenced by the vital zinc (Zn) status, and Zn deficiency triggers lymphopenia; however, the mechanisms underlying Zn-mediated lymphocyte maintenance remain elusive. Here we investigated ZIP10, a Zn transporter expressed in the early B-cell developmental process. Genetic ablation of Zip10 in early B-cell stages resulted in significant ...
Jadidian Armon A Department of Otolaryngology-Head and Neck Surgery, University of Florida College of Medicine, Gainesville, Florida, - - 2014
Mitoquinone (MitoQ) attenuates aminoglycoside (AG)-induced upregulation of the proapoptotic molecules Bak and harakiri (Hrk) and decreases the percentage of apoptotic House Ear Institute Organ of Corti 1 (HEI-OC1) cells. The primary mechanism of AG ototoxicity is the formation of reactive oxygen species, which leads to hair cell death via apoptotic ...
Nguyen Khanh-Quynh N KQ Rutgers Biomedical and Health Sciences, United - - 2014
MERTK, a member of the TAM (TYRO3, AXL, and MERTK) receptor tyrosine kinases, has complex and diverse roles in cell biology. On the one hand, knockout of MERTK results in age-dependent autoimmunity characterized by failure of apoptotic clearance, while on the other, MERTK overexpression in cancer drives classical oncogene pathways ...
Jang Wooyoung W Department of Neurology, Gangneung Asan Hospital, University of Ulsan College of Medicine, Bangdong-ri, Sacheon-myeon, Gangneung-si, Gangwon-do 210-711, Republic of Korea. Electronic address: - - 2014
Dysregulation of the autophagy pathway has been suggested as an important mechanism in the pathogenesis of Parkinson's disease (PD). Therefore, modulation of autophagy may be a novel strategy for the treatment of PD. Recently, an active form of vitamin D3 has been reported to have neuroprotective properties. Therefore, we investigated ...
Gulan Ljiljana L Faculty of Natural Sciences, University of Pristina, Lole Ribara 29, Kosovska Mitrovica 38200, Serbia - - 2014
The survey of natural radioactivity in Kosovo and Metohija involves 180 indoor (220)Rn measurements. They were performed either in living rooms or in bedrooms of 127 individual, rural type houses, using a passive method with application of CR-39 solid-state nuclear track detectors. Detectors were deployed at a distance of >10 ...
Tao Jie J Jie Tao, Xin-Sen Xu, Kai Qu, Rui-Tao Wang, Fan-Di Meng, Ji-Chao Wei, Shun-Bin Dong, Lin Wang, Chang Liu, Department of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, - - 2014
To investigate the effect of knockdown of Forkhead box M1 (FoxM1) on the proliferation and invasion capacities of human gallbladder carcinoma (GBC)-SD cells. Four FoxM1 shRNAs were transfected into GBC-SD cells with Lipofectamine 2000 to select the appropriate shRNA for down-regulation of FoxM1. A recombinant lentivirus for shFoxM1 (Lv-shFoxM1), which ...
Zych Jaiesa J 1 Instituto Carlos Chagas , Fiocruz-Paraná, Curitiba, Brazil - - 2014
Although fibroblasts and multipotent stromal/stem cells, including adipose-derived stromal cells (ADSCs), have been extensively studied, they cannot be clearly distinguished from each other. We, therefore, investigated the cellular and molecular characteristics of ADSCs and fibroblasts. ADSCs and fibroblasts share several morphological similarities and surface markers, but were clearly found to ...
Rock Jason R JR Department of Anatomy, University of California, San Francisco, CA 94143, USA. Electronic address: - - 2014
Mammalian lungs are comprised of conducting airways and alveoli. How the distinct epithelial linings of these two zones are differentially specified and maintained is not fully understood. In this issue of Developmental Cell, two groups find critical roles for the Hippo pathway in regulation of lung progenitor cell differentiation.
Chen Jieyan V JV Department of Biomedical Sciences, Florida State University, 1115 West Call Street, Tallahassee, FL 32306-4300, - - 2014
In this issue of Developmental Cell, Fong et al. (2014) present evidence for a model of centriole duplication whereby the cartwheel-the starting building block in centriole biogenesis-assembles within the lumen of the mother centriole before templating the daughter centriole to ensure a single duplication event per cell cycle.
Bergström G G Division of Biotechnology, Dept. of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden. - - 2014
Macroporous gelatin beads (CultiSpher™ microcarriers) provide a convenient method for rapidly and reliably introducing cells cultured ex situ into a microfluidic device, where the spheres create a 3D environment for continued cell proliferation. We demonstrate the usefulness of this technique with a proof-of-concept viability analysis of cardiac cells after treatment ...
Lodhia Kunal A KA Department of Oncology, University of Oxford, Oxford, - - 2014
It is common practice to irrigate the operative site following tumor resection during major head and neck surgery. A variety of irrigation solutions are used, but there are few data on their relative efficacies in this context. The effect of different irrigation solutions on cell survival was assessed by clonogenic ...
Wei Jianfeng - - 2014
We investigated the effects of all-trans retinoic acid (ATRA) and arsenic trioxide (ATO), alone and in combination, on apoptosis and intracellular calcium concentration in hepatocellular carcinoma (HepG2) cells. We used HepG2 cells to test the effects of ATRA and ATO, individually and in combination, on cell proliferation, apoptosis, and intracellular-free ...
Subrahmanyam Priyanka B PB Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum Cancer Center, Baltimore, MD - - 2014
NKT cells are a unique subset of T cells that recognize glycolipid Ags presented in the context of CD1d molecules. NKT cells mount strong antitumor responses and are a major focus in developing effective cancer immunotherapy. It is known that CD1d molecules are constantly internalized from the cell surface, recycled ...
Chiu Yi-Kai YK Genomics Research Center, Academia Sinica, Taipei 115, Taiwan; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, - - 2014
Ag-primed B cells that result from an immune response can form either memory B cells or Ab-secreting plasma cells; however, the molecular machinery that controls this cellular fate is poorly understood. In this study, we show that activated B cell factor-1 (ABF-1), which encodes a basic helix-loop-helix transcriptional repressor, participates ...
Rahmani Mohamed M Internal Medicine, Virginia Commonwealth - - 2014
Purpose: To explore the efficacy and define mechanisms of action of co-administration of the PI3K/mTOR inhibitor BEZ235 and pan-HDAC inhibitor panobinostat in DLBCL cells. Experimental Design: Various DLBCL cells were exposed to panobinostat and BEZ235 alone or together after which apoptosis and signaling/survival pathway perturbations were monitored by flow cytometry ...
Bracken Cameron P CP Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA, Australia Discipline of Medicine, University of Adelaide, Adelaide, SA, - - 2014
The microRNAs of the miR-200 family maintain the central characteristics of epithelia and inhibit tumor cell motility and invasiveness. Using the Ago-HITS-CLIP technology for transcriptome-wide identification of direct microRNA targets in living cells, along with extensive validation to verify the reliability of the approach, we have identified hundreds of miR-200a ...
Chi Liyi L Department of Neurology, The 451st Hospital of People's Liberation Army, Xi'an, Shaanxi 710054, P.R. - - 2014
Berberine (BBR) is a botanical alkaloid that has been reported to have effects in cardiovascular diseases; however, the mechanisms involved are not yet fully understood. In the present study, the protective effects of BBR were evaluated, and the underlying molecular mechanisms were investigated. The effects of a combination of atorvastatin ...
Zhang Hong - - 2014
S-allyl mercaptocysteine (SAMC), a water-soluble component derived from garlic, has been found to exert multi-antitumor activities. This study was to investigate the responsible molecular mechanisms of SAMC in human breast cancer cell lines. Sulforhodamine B assay was used to determine cell viability, flow cytometry was applied for the analysis of ...
Tahata Shinichi S Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, - - 2014
The cytocidal effect of pyrrolidine dithiocarbamate (PDTC) was investigated by focusing on cell viability, cell cycle arrest and apoptosis induction in small-cell lung cancer (SCLC) cell lines (NCI-H196 and NCI-H889). PDTC exhibited a much stronger dose-dependent cytotoxic activity against NCI-H196 compared to NCI-H889, while no such activity was observed in ...
Kurt Birguel B Institute of Physiology, University of Regensburg, Regensburg, Germany - - 2014
On the basis of previous observations that deletion of the von Hippel-Lindau protein (pVHL) in juxtaglomerular (JG) cells of the kidney suppresses renin and induces erythropoietin expression, this study aimed to characterize the events underlying this striking change of hormone expression. We found that renin cell-specific deletion of pVHL in ...
Sansanwal Poonam P California Pacific Medical Center Research Institute, San Francisco, California; poonam.sansanwal@gmail.com - - 2014
Nephropathic cystinosis, characterized by accumulation of cystine in the lysosomes, is caused by mutations in CTNS. The molecular and cellular mechanisms underlying proximal tubular dysfunction and progressive renal failure in nephropathic cystinosis are largely unclear, and increasing evidence supports the notion that cystine accumulation alone is not responsible for the ...
Deakin Nicholas O NO Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY - - 2014
Polarized cell migration is essential for normal organism development and is also a critical component of cancer cell invasion and disease progression. Directional cell motility requires the coordination of dynamic cell-extracellular matrix interactions as well as repositioning of the Golgi apparatus, both of which can be controlled by the microtubule ...
Wang Chao-Wen CW Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan - - 2014
Stationary phase (stat-phase) is a poorly understood physiological state under which cells arrest proliferation and acquire resistance to multiple stresses. Lipid droplets (LDs), organelles specialized for cellular lipid homeostasis, increase in size and number at the onset of stat-phase. However, little is known about the dynamics of LDs under this ...
Sim Jingwei J Physiological Laboratory, University of Cambridge, Downing Street, Cambridge CB2 3EG, - - 2014
Oligodendrocyte precursor cells are the primary source of myelinating oligodendrocytes in the neonatal CNS. In a recent issue of Cell, Yuen and coworkers (2014) show that hypoxia-inducible factors and Wnt7a/7b act in developing white matter, promoting angiogenesis in anticipation of the metabolic demands of oligogenesis, before finally allowing precursor differentiation.
Jun Jin-Gon JG Department of Anatomy and Cell Biology, Hyogo College of - - 2014
Neurons influence renal function and help to regulate fluid homeostasis, blood pressure and ion excretion. Intercalated cells (ICCs) are distributed throughout the renal collecting ducts and help regulate acid/base equilibration. Because ICCs are located among principal cells, it has been difficult to determine the effects that efferent nerve fibers have ...
Bokhari Amber A AA Obstetrics and Gynecology, Uniformed Services - - 2014
Increased expression of transforming growth factor-β (TGF-β) isoforms in human endometrial cancer correlates with decreased survival and poor prognosis. Progesterone has been shown to exert a chemoprotective effect against endometrial cancer and previous animal models have suggested that these effects are accompanied by changes in TGF-β. The goal of this ...
Mansoor Tayyab A TA Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon , 1649-003 Lisbon, - - 2014
6-Acetonyldihydrochelerythrine (1), a benzophenanthridine alkaloid, isolated from the methanol extract of Zanthoxylum capense, displayed potent cytotoxic activity in human HCT116 and SW620 colon carcinoma cells, to a higher extent than 5-fluorouracil (5-FU), the cornerstone chemotherapeutic agent in colon cancer. Cytotoxicity of 1 was evaluated by MTS, lactate dehydrogenase (LDH), and ...
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