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Andrés Zaida Z Instituto de Recursos Naturales y Agrobiologia de Sevilla, Consejo Superior de Investigaciones Cientificas, 41012 Seville, - - 2014
Stomatal movements rely on alterations in guard cell turgor. This requires massive K(+) bidirectional fluxes across the plasma and tonoplast membranes. Surprisingly, given their physiological importance, the transporters mediating the energetically uphill transport of K(+) into the vacuole remain to be identified. Here, we report that, in Arabidopsis guard cells, ...
Abdelraouf Kamilia K Depatment of Pharmacological and Pharmaceutical - - 2014
Polymyxin B is increasingly used as a treatment of last resort against multidrug resistant Gram-negative infections. Using a mammalian kidney cell line, we demonstrated polymyxin B uptake into proximal tubular epithelial cells was saturable and occurred primarily through the apical membrane, suggesting the involvement of transporters in the renal uptake ...
Kinoshita Taisuke T Keio University, - - 2014
Reactivation of the endogenous telomerase reverse transcriptase (TERT) catalytic subunit and telomere elongation occur during the reprogramming of somatic cells to induced pluripotent stem (iPS) cells. However, the role of TERT in the reprogramming process is unclear. To clarify its function, the reprogramming process was examined in TERT-KO somatic cells. ...
Yeh Tzyy-Harn TH School of Pharmacy - - 2014
Abstract In some cancer cells, translocation of cytochrome c (Cyt c) from mitochondria to the cytoplasma is inhibited. This inhibition prevents cells from undergoing apoptotic cell death and can lead to uncontrolled cell growth. Increasing cytoplasmic concentration of Cyt c can induce apoptosis in cancer cells as a strategy of ...
Dowling Mark R MR Division of Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, - - 2014
Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic ...
Cussat-Blanc Sylvain S Brandeis - - 2014
Abstract All multicellular living beings are created from a single cell. A developmental process, called embryogenesis, takes this first fertilized cell down a complex path of reproduction, migration, and specialization into a complex organism adapted to its environment. In most cases, the first steps of the embryogenesis take place in ...
Millen Kathleen J KJ Seattle Children's Research Institute, Seattle, WA - - 2014
Model organism studies have demonstrated that cell fate specification decisions play an important role in normal brain development. Their role in human neurodevelopmental disorders, however, is poorly understood, with very few examples described. The cerebellum is an excellent system to study mechanisms of cell fate specification. Although signals from the ...
Li Guijun - - 2014
Recently, ultrathin crystalline silicon solar cells have gained tremendous interest because they are deemed to dramatically reduce material usage. However, the resulting conversion efficiency is still limited by the incomplete light absorption in such ultrathin devices. In this letter, we propose ultrathin a-Si/c-Si tandem solar cells with an efficient light ...
Hong In-Kee IK Kangwon National University, Korea, Republic - - 2014
Despite the importance of multiple tetraspanin proteins in cancer invasion and metastasis, little is known about the role and significance of tetraspanin CD81 in these processes. In the present study, we examined CD81 effects on melanoma cell invasiveness and metastasis. Transfection of CD81 into melanoma cells, lacking endogenous CD81 expression, ...
Matkar Smita S Abramson Family Cancer Research Institute, Department of Cancer Biology, University of Pennsylvania Perelman School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, - - 2014
It is unclear whether the antiproliferative/proapoptotic activity of oncogenes can be pharmacologically reactivated in cancer cells. In this issue of Cancer Cell, Liu and colleagues report that a proteasome inhibitor reactivates an MLL-AF4 controlled antitumor program to kill leukemia cells in an oncogene dose- and cell type-dependent manner.
Watanabe Kazuhide K Department of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA 92697, - - 2014
Epithelial cells possess remarkable plasticity, having the ability to become mesenchymal cells through alterations in adhesion and motility (epithelial-to-mesenchymal transition [EMT]). However, how epithelial plasticity is kept in check in epithelial cells during tissue development and regeneration remains to be fully understood. Here we show that restricting the EMT of ...
Li Qiongshu Q Department of Immunology, College of Basic Medical Science, Jilin University, Changchun, Jilin 130021, P.R. - - 2014
Mucin1 (MUC1) is a transmembrane glycoprotein that plays a key role as an oncogene in the tumorigenesis of many human adenocarcinomas. However, the role of MUC1 in human hepatocellular carcinoma (HCC) progression remains unclear. In the present study, we silenced MUC1 to investigate its effect on the human HCC cell ...
Wang Hanghui H Department of Ultrasound, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, P.R. - - 2014
The specific and efficient delivery of small interfering RNA (siRNA) into cancer cells in vivo remains a major obstacle. In this study, we investigated whether ultrasound-targeted microbubble destruction (UTMD) combined with dual targeting of HSP72 and HSC70 in prostate cancer cell lines improve the specific and efficient cell uptake of siRNA, ...
Barry C C UCL Research Department of Cell & Developmental Biology, Gower Street, London, WC1E 6BT, UK. Electronic address: - - 2014
The ability to self-localise and to navigate to remembered goals in complex and changeable environments is crucial to the survival of many mobile species. Electrophysiological investigations of the mammalian hippocampus and associated brain structures have identified several classes of neurons which represent information about an organism's position and orientation. These ...
Hayashi Katsuhiko K Graduate School of Medicine, Kyoto University, Kyoto, Japan; CiRA, Graduate School of Medicine, Kyoto University, Kyoto, Japan; PRESTO, Japan Science and Technology Agency, Saitama, - - 2014
Pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are able to differentiate into all cell lineages of the embryo proper, including germ cells. This pluripotent property has a huge impact on the fields of regenerative medicine, developmental biology and reproductive engineering. Establishing the ...
Godinho Susana A SA 1] Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana-Farber Cancer Institute and Pediatric Hematology/Oncology, Children's Hospital, Boston, Massachusetts 02115, USA [2] Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA [3] Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK (S.A.G.); Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA - - 2014
Centrosome amplification has long been recognized as a feature of human tumours; however, its role in tumorigenesis remains unclear. Centrosome amplification is poorly tolerated by non-transformed cells and, in the absence of selection, extra centrosomes are spontaneously lost. Thus, the high frequency of centrosome amplification, particularly in more aggressive tumours, ...
Czuczman Mark A MA 1] Cell Biology Program, Hospital for Sick Children, Toronto, Ontario M5G0A4, Canada [2] Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S1A8, - - 2014
Efferocytosis, the process by which dying or dead cells are removed by phagocytosis, has an important role in development, tissue homeostasis and innate immunity. Efferocytosis is mediated, in part, by receptors that bind to exofacial phosphatidylserine (PS) on cells or cellular debris after loss of plasma membrane asymmetry. Here we ...
Treutlein Barbara B 1] Departments of Bioengineering and Applied Physics, Stanford University School of Medicine and Howard Hughes Medical Institute, Stanford, California 94305, USA - - 2014
The mammalian lung is a highly branched network in which the distal regions of the bronchial tree transform during development into a densely packed honeycomb of alveolar air sacs that mediate gas exchange. Although this transformation has been studied by marker expression analysis and fate-mapping, the mechanisms that control the ...
Mouradian Michael M Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, - - 2014
Docosahexaenoic acid (DHA; C22:6n-3) depresses mammary carcinoma proliferation and growth in cell culture and in animal models. The current study explored the role of interrupting bioenergetic pathways in BT-474 and MDA-MB-231 breast cancer cell lines representing respiratory and glycolytic phenotypes, respectively and comparing the impacts of DHA with a non-transformed ...
Wang Jungang J College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of - - 2014
Clostridium straminisolvens (CSK1) is a novel cellulolytic bacterium isolated from a cellulose-degrading bacterial community MC1. In this study, the influence of the following cell disruption and elution methods on CSK1cellulase release was investigated: (1) freezing-thawing, (2) ultrasonication, (3) elution, (4) freezing-thawing following elution, (5) ultrasonication following elution, and lastly (6) ...
Kolos E C EC Biomedical Engineering, School of AMME J07, University of Sydney, Sydney, NSW, 2006, Australia, - - 2014
Tissue engineering offers a novel route for repairing damaged or diseased tissue by incorporating the patient's own healthy cells or donated cells into temporary scaffolds that act as a matrix for cell cultivation. Tissue scaffolds that are biocompatible and are porous with interconnected porous channels for cell ingrowth with a ...
Morimoto Kazuya K Department of Urology, Osaka City University Graduate School of Medicine, Osaka, - - 2014
NEDD9 is one of the Crk-associated substrate (Cas) family proteins that mediate downstream signaling processes including cytoskeletal organization, cell-cycle and tumorigenesis. While NEDD9 plays a crucial role in epithelial-mesenchymal transition (EMT), the functional mechanism underlying NEDD9-mediated EMT in prostate cancer (PCa) remains uncertain. The expression levels of NEDD9 and its ...
Roy Sudipa S SS Department of Biochemistry, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, - - 2014
Proteasome inhibition is associated with substantial antitumor effects in preclinical models of multiple myeloma (MM) as well as in patients. However, results of recent clinical trials to evaluate the effect of the proteasome inhibitor Bortezomib (Velcade(®), also called PS-341) in MM patients have shown limited activity when used as a ...
Hosogi Shigekuni S Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan; Japan Institute for Food Education and Health, Heian Jogakuin (St. Agnes') University, Kyoto, - - 2014
The purpose of the present study was to clarify roles of cytosolic chloride ion (Cl(-) ) in regulation of lysosomal acidification [intra-lysosomal pH (pHlys )] and autophagy function in human gastric cancer cell line (MKN28). The MKN28 cells cultured under a low Cl(-) condition elevated pHlys and reduced the intra-lysosomal ...
Gričar Jožica J Slovenian Forestry Institute, Vecna pot 2, SI-1000 Ljubljana, - - 2014
Despite its major economic importance and the vulnerability of Picea abies (L.) H. Karst. to climate change, how its radial growth at intra-annual resolution is influenced by weather conditions in forest stands with a high production capacity has scarcely been explored. Between 2009 and 2011, phenological variation in seasonal cambial ...
Pan Shuhua S College of Life Science, Zhejiang Sci-Tech University, Xiasha Higher Education Zone, Hangzhou, 310018, Zhejiang Province, - - 2014
Oleanolic acid (OA) derivatives exhibit numerous pleiotropic effects in many cancers. The present study aimed to investigate the molecular mechanisms of 5'-amino-oleana-2,12-dieno[3,2-d]pyrimidin-28-oic acid (compound 4) and oleana-2,12-dieno[2,3-d]isoxazol-28-oic acid (compound 5) inducing apoptosis in human leukemia K562 cell. We investigated the effects of the compounds on K562 cell growth, apoptosis and ...
Zhang Yuzhen Y Department of Cardiology, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou, - - 2014
Objectives: Cellular senescence may play an important role in the pathology of heart aging. We aimed to explore whether induced pluripotent stem cells (iPSCs) could inhibit cardiac cellular senescence via a paracrine mechanism. Methods: We collected iPSC culture supernatant, with or without oxidative stress, as conditioned medium (CM) for the ...
Shojaee Sousan - - 2014
Abstract Background: Despite the wide range of investigations on the therapeutic potentials of herbal extracts in cancer cell lines, there is not usually enough data on their probable effects on normal cells. Recently, both anti-tumoral and anti-oxidant properties of Scutellaria litwinowii extracts have been reported on different cancer cell lines. ...
Qiu Ying Y Department of Dermatology, Shandong Ji-ning No.1 People's Hospital, Ji-ning City, Shandong Province, 272011, P.R. China. Electronic address: - - 2014
Here we studied the role of mitochondrial permeability transition pore (mPTP) opening in curcumin's cytotoxicity in melanoma cells. In cultured WM-115 melanoma cells, curcumin induced mitochondrial membrane potential (MPP) decrease, cyclophilin-D (CyPD)-adenine nucleotide translocator 1 (ANT-1) (two mPTP components) mitochondrial association and cytochrome C release, indicating mPTP opening. The mPTP ...
Sun Qinru Q Department of Human Anatomy and Embryology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, - - 2014
Amyloid β (Aβ) plays a critical role in the pathogenesis of Alzheimer disease (AD). Studies indicate that Aβ causes reactive oxygen species (ROS) generation, mitochondrial dysfunction and neurons loss in vivo and in vitro. Taurine, a naturally occurring β-amino acid in the brain, has been demonstrated to have neuroprotective properties. ...
Zhao Yu Y Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, People's Republic of China. Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing, People's Republic of - - 2014
Advances in three-dimensional (3D) printing have enabled the direct assembly of cells and extracellular matrix materials to form in vitro cellular models for 3D biology, the study of disease pathogenesis and new drug discovery. In this study, we report a method of 3D printing for Hela cells and gelatin/alginate/fibrinogen hydrogels ...
Yuan Xiao-Hua XH Maternity Department, Shaanxi Provincial People's Hospital, Xi'an, 710068, - - 2014
Glucocorticoids have been widely used in clinical application for anti-inflammatory and immunosuppressive function. Previous study reported that glucocorticoids adversely affect the reproductive system and can directly act on ovary. Here, we found that progesterone production induced by dexamethasone requiring activation of caspase-3 which may mediate differentiation and apoptosis of granulosa ...
Chen Jui-Chieh JC National Institute of Cancer Research, National Health Research Institutes, Zhunan, - - 2014
Accumulating evidence is revealing an important role of microRNA (miRNA) in tumor progression and chemotherapeutic resistance. Dicer is a cytoplasmic endoribonuclease type III crucial for production of mature miRNAs. The aberrant expression of Dicer has also been reportedly associated with clinical aggressiveness, prognosis, and patient survival in various cancer types. ...
Mochizuki Toshiaki T Developmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna, Okinawa, 904-0495, - - 2014
The vertebrate lens is a transparent, spheroidal tissue, located in the anterior region of the eye that focuses visual images on the retina. During development, surface ectoderm associated with the neural retina invaginates to form the lens vesicle. Cells in the posterior half of the lens vesicle differentiate into primary ...
Campbell Jonathan J JJ Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK. jjc55@cam.ac.uk - - 2014
Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring. These processes are mediated in part through Jak/Stat signalling pathways, which can be deregulated in breast cancer. Synthetic in vitro analogues ...
Glass Rainer R Neurosurgical Research, Department of Neurosurgery, University Clinics Munich, 81377, Munich, Germany, - - 2014
Primary brain tumours (gliomas) initiate a strong host response and can contain large amounts of immune cells (myeloid cells) such as microglia and tumour-infiltrating macrophages. In gliomas the course of pathology is not only controlled by the genetic make-up of the tumour cells, but also depends on the interplay with ...
Hallwass Mariana M Laboratory of Virology, Department of Plant Sciences, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands; Instituto de Ciências Biológicas, Departamento de Biologia Celular, Universidade de Brasília, ICC - Sul Asa Norte 70910-900, Brasília, DF, - - 2014
Although the Sw-5 gene cluster has already been cloned, and Sw-5b identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr)-determinant still has not been identified. Whereas Nicotiana tabacum 'SR1' plants transformed with a copy of Sw-5b gene were immune without producing ...
Wei Ling L State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, - - 2014
The transcription factor SoxE is mainly expressed in the gonad and involved in the regulation of gonad development and sex determination in animals. Here, we used the silkworm ovary-derived BmN4-SID1 cell line to survey the roles of the silkworm SoxE protein (BmSoxE) and predict its candidate binding targets. RNAi-mediated silencing ...
Bu Ye - - 2014
Protein tyrosine phosphatases (PTPs) such as SHP-1, SHP-2 and CD45 are involved in hematopoiesis, but the function of many PTPs is not well characterized in vivo. Here we have identified Ptpn9a, an ortholog of human PTPN9, as a crucial regulator of erythroid cell development in zebrafish embryos. ptpn9a, but not ...
Wang Juan-Juan JJ Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, People's Republic of - - 2014
O-mannosylation dependent on protein O-mannosyltransferase (Pmt) family is an essential posttranslational modification process in eukaryotes but their connection to the biocontrol potential of a filamentous entomopathogen against arthropod pests has not been understood. Here, we characterized the functions of three Pmt orthologues (Pmt1, Pmt2 and Pmt4) in the Pmt family ...
Padmanabhan Jagannath - - 2014
Nanopatterning of biomaterials is rapidly emerging as a tool to engineer cell function. Bulk metallic glasses (BMGs), a class of biocompatible materials, are uniquely suited to study nanopattern-cell interactions as they allow for versatile fabrication of nanopatterns through thermoplastic forming (TPF). Work presented here employs nanopatterned BMG substrates to explore ...
Almeida M B MB Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, - - 2014
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and ...
Smeltzer Jacob J Hematology, Mayo - - 2014
Transformation of follicular lymphoma (FL) is a critical event associated with a poor prognosis. The role of the tumor microenvironment in previous transformation studies has yielded conflicting results. To define cell subtypes associated with transformation, we examined tissue specimens at diagnosis from patients with FL that later transformed and, using ...
Maiseyeu Andrei - - 2014
A new quantum dot (QD) PEGylated micelle laced with phosphatidylserine (PS) for specific scavenger receptor-mediated uptake by macrophages is reported. The size and surface chemistry of PS-QD micelles were characterized by standard methods and the effects of their physicochemical properties on specific targeting and uptake were comprehensively studied in a ...
Martirosyan Varsik V Department of Biotechnology and Biophysics, Life Sciences International Postgraduate Educational Center , Yerevan , - - 2014
Abstract The aim of the present work is to study the time-dependent effects of mechanical vibration (MV) at infrasound (IS) frequency at 4 and 8 Hz on E. coli K-12 growth by investigating the cell proliferation, using radioactive [(3)H]-thymidine assay. In our previous work it was suggested that the aqua medium ...
Napso Tina T Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Mount Carmel, 31905 Haifa, - - 2014
Zebularine, a potent DNA methyltransferase inhibitor, is potentially able to influence gene regulation and thereby alters cell behavior. This study illustrates the effect of zebularine on human squamous cell carcinoma (SCC-9 and SCC-25) in vitro. The results indicated that zebularine significantly (P<0.05) reduced viability and DNA synthesis of treated cancer cells, ...
García-Martín A A Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz and Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (RETICEF)-Instituto de Salud Carlos III, Madrid, Spain, - - 2014
Parathyroid hormone (PTH)-related protein (PTHrP) is an important regulator of bone remodelling, apparently by acting through several sequence domains. We here aimed to further delineate the functional roles of the nuclear localization signal (NLS) comprising the 88-107 amino acid sequence of PTHrP in osteoblasts. PTHrP mutants from a human PTHrP ...
Zhang Dongyun - - 2014
Cell migration is a dynamic process that is central to a variety of physiological functions as well as disease pathogenesis. The modulation of cell migration by p27 has been reported, but the exact mechanism(s) whereby p27 intersects with downstream effectors that control cell migration have not been elucidated. By systematically ...
Yang Haiping H Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, - - 2014
Bronchopulmonary dysplasia (BPD) is a major threat to the health of premature infants yet its pathogenesis is not fully understood. Epithelial-mesenchymal transition (EMT) of lung epithelial cells may lead to BPD. To investigate the potential occurrence of EMT in a newborn rat model of BPD. Newborn rats were exposed to ...
Daien Claire I CI Department of Rheumatology, Lapeyronie hospital and Montpellier I University, Montpellier, France; Members of TNF-alpha laboratory, UMR5535, IGMM, CNRS, Montpellier, - - 2014
Objective: Regulatory IL-10 producing B cells (B10) have been shown to prevent and cure collagen-induced arthritis in mice. In human, very little is known about B10 cells in rheumatoid arthritis (RA). Several B cell subsets such as CD24(hi) CD38(hi) , CD24(hi) CD27(+) and CD5(+) B cells were suggested to be ...
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