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Axelrod Jeffrey D JD Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA - - 2014
A diverse group of researchers working on both plant and animal systems met at a Company of Biologists workshop to discuss 'Coordinating Cell Polarity'. The meeting included considerable free discussion as well as presentations exploring the ways that groups of cells in these various systems achieve coordinated cell polarity. Here, ...
Tubin Slavisa - - 2014
Abstract Purpose: This in vitro study evaluated the ability of prostate adenocarcinoma (ADC) cells to induce radiation-induced bystander effect (RIBE) exploring the factors that may be responsible and affect its intensity. The idea was to mimic a strong, clinically applicable RIBE that could lead to the development of innovative approaches ...
Faller K K Neurology Unit, Small Animal Hospital, School of Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Scotland, United - - 2014
A 4-year old spayed male domestic shorthair cat was presented with a history of circling and behavioral changes. Neurologic examination showed mild proprioceptive deficits. The lesion was localized in the forebrain, and magnetic resonance imaging revealed the presence of a large midline intracranial mass extending from the frontal lobe to ...
Park Eun-Sil ES Department of Biology, Laboratory of Aging and Infertility Research, Northeastern University, Boston, Massachusetts 02115, - - 2014
Several laboratories have independently isolated mitotically active germ cells, termed female germline stem cells (fGSCs) or oogonial stem cells (OSCs), from adult mouse ovaries. However, a recent study from Liu and colleagues using Ddx4-Cre;Rosa26 reporter mice concluded that such germ cells do not exist. Given the disparity in conclusions drawn ...
Qin Qin - - 2014
BackgroundSurvivin is overexpressed in cancer cells and plays a crucial role in apoptosis evasion. YM155, a small-molecule inhibitor of survivin, could enhance the cytotoxicity of various DNA-damaging agents. Here, we evaluated the radiosensitizaion potential of YM155 in human esophageal squamous cell carcinoma (ESCC).MethodsCell viability was determined by CCK8 assay. The ...
Kulkarni Rajan P RP Department of Bioengineering, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California Los Angeles, 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, California 90095, USA. - - 2014
We highlight recent reports using microfluidic systems to perform single-cell analysis. It has been demonstrated on numerous occasions that population averages are often not representative of single-cell behavior. These differences in behavior can be caused by stochastic fluctuations in temporal response, changes in the surrounding instructive environment, or hard-coded genetic ...
Li Yubin Y Department of Biosynthesis & The Key Laboratory of Smart Drug Delivery, Ministry of Education, School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of - - 2014
Poly(amidoamine) (PAMAM) dendrimers are proposed as one of the most promising nanomaterials for biomedical applications because of their unique tree-like structure, monodispersity and tunable properties. In this study, we found that PAMAM dendrimers could induce the formation of autophagosomes and the conversion of microtubule-associated protein 1 light chain 3 (LC3) ...
Trost A A Ophthalmology/Optometry, Paracelsus Medical University, Müllner Hauptstrasse 48, 5020, Salzburg, Austria, - - 2014
Doublecortin (DCX) is predominantly expressed in neuronal precursor cells and young immature neurons of the developing and adult brain, where it is involved in neuronal differentiation, migration and plasticity. Moreover, its expression pattern reflects neurogenesis, and transgenic DCX promoter-driven reporter models have been previously used to investigate adult neurogenesis. In ...
Feng Xiujing X Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, 210014, - - 2014
Production of human α-lactalbumin (hα-LA) transgenic cloned dairy goats has great potential in improving the nutritional value and perhaps increasing the yield of dairy goat milk. Here, a mammary-specific expression vector 5A, harboring goat β-lactoglobulin (βLG) promoter, the hα-LA gene, neo(r) and EGFP dual markers, was constructed. Then, it was ...
Chen Zhi-Jie - - 2014
The discovery of hydrogen sulfide (H2S) as a novel gasotransmitter for cell signaling and other pathophysiological processes has spurred tremendous interest in developing analytical methods for its detection in biological systems. Herein, we report the development of a highly responsive and selective genetically encoded H2S probe, hsGFP, for the detection ...
Egger Michael M University of Louisville, Surgery, Louisville, Kentucky, United States ; - - 2014
Melanoma exhibits variable resistance to the alkylating agent temozolomide (TMZ). We evaluated the potential of adenovirus expressing forkhead human transcription factor like 1 triple mutant (Ad-FKHRL1/TM) to sensitize melanoma cells to TMZ. Four melanoma cell lines were treated with Ad-FKHRL1/TM and TMZ, alone or in combination. Apoptosis was assessed by ...
Zhu Jiaoyan J Institute of Polymer Optoelectronic Materials & Devices, State Key Laboratory of Luminescent Materials & Devices, South China University of Technology, Guangzhou 510640, People's Republic of - - 2014
Interface states influence the operation of nanocrystal (NC) solar cell carrier transport, recombination and energetic mechanisms. In a typical CdTe NC solar cell with a normal structure of a ITO/p-CdTe NCs/n-acceptor (or without)/Al configuration, the contact between the ITO and CdTe is a non-ohm contact due to a different work ...
Koekemoer T C TC Department of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, Port Elizabeth, 6031, South - - 2014
To investigate anti-proliferatory activity of a selected N,N-[(8-hydroxyquinoline)methyl]-substituted benzylamine (JLK1486) on melanoma cells and to characterize its mechanism of cell population growth inhibition. In vitro cultures of B16F10 (mouse melanoma) cells were used as a model to characterize anti-proliferatory activity of JLK1486 using MTT growth assay, trypan blue viability assessment, ...
Chera Simona S Department of Genetic Medicine &Development, Faculty of Medicine, University of Geneva, 1 rue Michel-Servet, 1211 Geneva-4, - - 2014
Total or near-total loss of insulin-producing β-cells occurs in type 1 diabetes. Restoration of insulin production in type 1 diabetes is thus a major medical challenge. We previously observed in mice in which β-cells are completely ablated that the pancreas reconstitutes new insulin-producing cells in the absence of autoimmunity. The ...
Chowdhury Jamil J ARC Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, - - 2014
In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance. For the plant cell to block penetration attempts, it must adapt its cell wall to withstand the physical and chemical ...
Fernandez-Garcia Elisa E Nanomaterials and Nanotechnology Research Center (CINN), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Oviedo (UO), El Entrego, Spain - - 2014
Ceramic/metal composites, cermets, arise from the idea to combine the dissimilar properties in the pure materials. This work aims to study the biocompatibility of new micro-nanostructured 3 Y-TZP/Ti materials with 25, 50 and 75 vol.% Ti, which have been successfully obtained by spark slasma sintering technology, as well as to correlate their ...
Ha Tae Kwang TK Department of Biological Sciences, KAIST, 335 Gwahak-ro, Yuseong-gu, Daejeon, 305-701, South Korea, - - 2014
Lithium chloride (LiCl), which is a specific inhibitor of glycogen synthase kinase-3β, is known to induce cell cycle arrest at the G2/M phase and to regulate apoptosis. To determine the potential of LiCl as a chemical additive to enhance specific productivity (q p) of recombinant Chinese hamster ovary (rCHO) cells ...
He Yingzi Y Department of Otolaryngology, Affiliated Eye and ENT Hospital, Fudan University, Shanghai, 200031, People's Republic of - - 2014
Aminoglycoside-induced hair cell loss is a major cause of hearing impairment in children and deserves more attention in medical research. Epigenetic mechanisms have been shown to protect hair cells from ototoxic drugs. In this study, we focused on the role of dimethylated histone H3K4 (H3K4me2) in hair cell survival. To ...
Charwat Verena V BioSensor Technologies, AIT Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. - - 2014
We have developed a lab-on-a-chip system for continuous and non-invasive monitoring of microfluidic cell cultures using integrated high-frequency contactless impedance spectroscopy. Electrically insulated microfabricated interdigitated electrode structures were embedded into four individually addressable microchambers to reliably and reproducibly detect cell-substrate interactions, cell viability and metabolic activity. While silicon nitride passivated ...
Turrioni Aps - - 2014
SUMMARY Objective : The aim of this study was to investigate the effects of transdentinal irradiation with different light-emitting diode (LED) parameters on odontoblast-like cells (MDPC-23). Methods and Materials : Human dentin discs (0.2 mm thick) were obtained, and cells were seeded on their pulp surfaces with complete culture medium ...
Wang Shunqiang S Department of Mechanical Engineering & Mechanics, Lehigh University, Bethlehem, PA 18015, USA. - - 2014
While substrates with nanopillars (NPs) have emerged as promising platforms for isolation of circulating tumor cells (CTCs), the influence of diameter and spacing of NPs on CTC capture is still unclear. In this paper, CTC-capture yield and cell behaviors have been investigated by using antibody functionalized NPs of various diameters ...
Ding Zhongyang Z Department of Surgery, Nanjing Medical University affiliated Wuxi People's Hospital, Wuxi, 214023, - - 2014
Akt2 overexpression correlates with chemoresistance of colorectal cancer (CRC). However, the cellular functions and precise signals elicited by Akt2 in LSCC have not been elucidated. Here, we transfected a CRC cell line HCT116 with Akt-2 targeted shRNA in order to establish a cell line with Akt2 knockdown. In vitro experiments ...
Diekmann Ulf U Institute of Clinical Biochemistry, Hannover Medical School , Carl-Neuberg-Str.1 , Hannover, Lower Saxony, Germany , 30625 , +495115322836 , +495115323584 ; - - 2014
Differentiation of pluripotent cells into endoderm related cell types initially requires in vitro gastrulation into the definitive endoderm. Most differentiation protocols are initiated from colonies of pluripotent cells complicating their adaption due to insufficiently defined starting conditions. The protocol described here was initiated from a defined cell number of dispersed ...
Sweeney Sinbad S Lung Cell Biology, Section of Pharmacology and Toxicology, National Heart and Lung Institute , Imperial College London, London , UK - - 2014
Abstract There can be significant variability between bioreactivity studies of nanomaterials that are apparently the same, possibly reflecting differences in the models used and differing sources of experimental material. In this study, we have generated two crystal forms of titanium dioxide nanoparticles (nano-TiO2), pure anatase and pure rutile to address ...
Schmitt J J Department of Biology, University of York, York, YO10 5DD, - - 2014
Background:Malignancy alters cellular complex lipid metabolism and membrane lipid composition and turnover. Here, we investigated whether tumorigenesis in cancer-derived prostate epithelial cell lines influences protein kinase C-linked turnover of ethanolamine phosphoglycerides (EtnPGs) and alters the pattern of ethanolamine (Etn) metabolites released to the medium.Methods:Prostate epithelial cell lines P4E6, LNCaP and ...
Kaistha B P BP Department of Medicine, Division of Gastroenterology, Endocrinology and Metabolism, Philipps University Marburg, Marburg, - - 2014
Background:Pancreatic ductal adenocarcinoma (PDAC) is among the most aggressive human malignancies with an overall 5-year survival rate of <5%. Despite significant advances in treatment of the disease during the past decade, the median survival rate (∼6 months) has hardly improved, warranting the need to identify novel targets for therapeutic approaches.Methods:Quantitative ...
Dinić Jelena J Institute for Biological Research, Department of Neurobiology, University of Belgrade, Belgrade, - - 2014
Diarylheptanoids belong to polyphenols, a group of plant secondary metabolites with multiple biological properties. Many of them display antioxidative, cytotoxic, or anticancer actions and are increasingly recognized as potential therapeutic agents. The aim of this study was to evaluate antioxidant and cytoprotective activity of two diarylheptanoids: platyphylloside 5(S)-1,7-di(4-hydroxyphenyl)-3-heptanone-5-O-β-D-glucopyranoside (1) and ...
Marinowic D R DR Biomedical Research Institute, Pontifícia Universidade Católica do Rio Grande do Sul. Brazil, Porto Alegre, 90610-000, Brazil, - - 2014
Human umbilical cord blood is an attractive source of stem cells; however, it has a heterogeneous cell population with few mesenchymal stem cells. Cell reprogramming induced by different methodologies can confer pluripotency to differentiated adult cells. The objective of this study was to evaluate the reprogramming of fibroblasts and their ...
He Chunbai - - 2014
Real-time measurement of intracellular pH in live cells is of great importance for understanding physiological/pathological processes and developing intracellular drug delivery systems. We report here the first use of nanoscale metal-organic frameworks (NMOFs) for intracellular pH sensing in live cells. Fluorescein isothiocyanate (FITC) was covalently conjugated to a UiO NMOF ...
Saito Mikako M Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan, - - 2014
The concentration-dependent effect of spermine was investigated on the spermine-induced generation of multilayer myotube sheets (MMTS) from mouse embryoid bodies (EBs). During spermine treatment for 24 h, a monolayer cell sheet that had already grown radially from the periphery of an EB was exfoliated. The exfoliation was inhibited by z-VAD.fmk, indicating ...
Liu Shunli S Department of Burn and Plastic, General Hospital of Jinan Military Region, 25 Shifan Road, Jinan, 250031, - - 2014
Cancer stem cells (CSCs) in most human tumors are commonly identified and enriched using similar strategies for identifying normal stem cells, including flow cytometry assays for side population, high aldehyde dehydrogenase (ALDH) activity, and CD133 positivity. Thus, development of a method for isolating a specific cancer using cancer-specific characteristic appears ...
Bian Xiao-Jie XJ Department of Urology, Fudan University Shanghai Cancer Center, No. 270, Dong'an Road, Shanghai, 200032, - - 2014
Dicer and Argonaute2 (Ago2) are critical components responsible not only for RNA interference but also for microRNA synthesis. The present study investigated the roles of Dicer and Ago2 in prostate cancer (Pca). First, the expression levels of Dicer and Ago2 in Pca tissues were determined by immunohistochemistry (IHC) and compared ...
Hocine Nora - - 2014
Abstract Purpose: To improve risk assessments associated with chronic exposure to Strontium-90 (Sr-90), for both the environment and human health, it is necessary to know the energy distribution in specific cells or tissue. Monte Carlo (MC) simulations codes are extremely useful tools for calculating deposition energy. The present work is ...
Park Ji Hun JH Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 305-701 - - 2014
Single-cell encapsulation promises the cytoprotection of the encased cells against lethal stressors, reminiscent of the sporulation process in nature. However, the development of a cytocompatible method for chemically mimicking the germination process (i.e., shell degradation on-demand) has been elusive, despite the shell degradation being pivotal for the practical use of ...
Bellini Marco M Institute of Chemistry of Organometallic Compounds, ICCOM-CNR, Polo Scientifico Area CNR, Via Madonna del Piano 10, 50019 Sesto Fiorentino - - 2014
Invited for this month's cover are the groups of Hansjörg Grützmacher at ETH in Zürich and Francesco Vizza at ICCOM-CNR in Florence. The image shows in an allegoric form an organometallic fuel cell releasing energy and fine chemicals produced through the electrooxidation of renewable diols. The Communication itself is available ...
Völker Timo T Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35043 Marburg - - 2014
The catalysis of bioorthogonal transformations inside living organisms is a formidable challenge-yet bears great potential for future applications in chemical biology and medicinal chemistry. We herein disclose highly active organometallic ruthenium complexes for bioorthogonal catalysis under biologically relevant conditions and inside living cells. The catalysts uncage allyl carbamate protected amines ...
Sattlegger Evelyn E Institute of Natural and Mathematical Sciences, Massey University, Albany, New - - 2014
Classic functions of the actin cytoskeleton include control of cell size and shape and the internal organization of cells. These functions are manifest in cellular processes of fundamental importance throughout biology such as the generation of cell polarity, cell migration, cell adhesion, and cell division. However, studies in the unicellular ...
Oakes Patrick W PW Institute for Biophysical Dynamics, James Franck Institute and Department of Physics, University of Chicago, Chicago, - - 2014
Cells generate mechanical stresses via the action of myosin motors on the actin cytoskeleton. Although the molecular origin of force generation is well understood, we currently lack an understanding of the regulation of force transmission at cellular length scales. Here, using 3T3 fibroblasts, we experimentally decouple the effects of substrate ...
Johnson Abby L AL University of - - 2014
Vitamin D3 receptor (VDR) signaling within the mammary gland regulates various postnatal stages of glandular development including puberty, pregnancy, involution, and tumorigenesis. Previous studies have shown that vitamin D3 treatment induces cell autonomous growth inhibition and differentiation of mammary epithelial cells in culture. Further, mammary adipose tissue serves as a ...
Alamri A A Oral Ecology Research Group, Faculty of Dentistry, Laval University, Quebec, QC, Canada; Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi - - 2014
Smoking cigarettes increases the risk of oral tissue damage leading to periodontal disease. Gingival fibroblasts, the predominant cell type inhabiting gingival connective tissue, play a critical role in remodeling and maintaining gingival structure. The objective of this study was to investigate the effect of long-term exposure to cigarette smoke on ...
Alex Jimi M JM Laboratory for Drug Design and Synthesis, Centre for Chemical and Pharmaceutical Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, - - 2014
A series of 17 analogs of 1-acetyl-4,5-dihydro(1H)pyrazoles (JP-1 to JP-17) bearing two aromatic rings at positions 3 and 5, either of which ought to be heterocyclic, were synthesized and evaluated for their anti-proliferative potential against breast cancer (MCF-7 and T-47D) and lung cancer (H-460 and A-549) cell lines for the ...
Lee Young-Hoon YH School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, - - 2014
We have previously shown that microRNAs (miRNAs) miR- 760, miR-186, miR-337-3p, and miR-216b stimulate premature senescence through protein kinase CK2 (CK2) downregulation in human colon cancer cells. Here, we examined whether these four miRNAs are involved in the replicative senescence of human lung fibroblast IMR-90 cells. miR-760 and miR-186 were ...
Edens Lance E - - 2014
Polymers and oligomers with conjugated phenylene ethynylene or thiophene ethynylene backbones have been shown to be potent antimicrobials. The mechanisms by which they act have been unclear, though AFM imaging of Escherichia coli cells before and after exposure to two such biocides, PPE-Th polymer and EO-OPE-1(C3), show their effects on ...
Bonde Sara S Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, - - 2014
The endeavor of exploiting arrays of vertical one-dimensional (1D) nanostructures (NSs) for cellular applications has recently been experiencing a pronounced surge of activity. The interest is rooted in the intrinsic properties of high-aspect-ratio NSs. With a height comparable to a mammalian cell, and a diameter 100-1000 times smaller, NSs should ...
Thomas A A IBR West Extension, School of Cancer Sciences, College of Medicine and Dentistry, University of Birmingham, Birmingham, - - 2014
Topoisomerase inhibitors are in common use as chemotherapeutic agents although they can display reduced efficacy in chemotherapy-resistant tumours, which have inactivated DNA damage response (DDR) genes, such as ATM and TP53. Here, we characterise the cellular response to the dual-acting agent, Alchemix (ALX), which is a modified anthraquinone that functions ...
Sarmento L M LM Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, - - 2014
Checkpoint kinase 1 (CHK1) is a key component of the ATR (ataxia telangiectasia-mutated and Rad3-related)-dependent DNA damage response pathway that protect cells from replication stress, a cell intrinsic phenomenon enhanced by oncogenic transformation. Here, we show that CHK1 is overexpressed and hyperactivated in T-cell acute lymphoblastic leukemia (T-ALL). CHEK1 mRNA ...
Orticello M M Institute of Biology, Molecular Medicine and Nanobiotechnology, CNR National Research Council, Rome, - - 2014
Mitotic proteins are attractive targets to develop molecular cancer therapeutics due to the intimate interdependence between cell proliferation and mitosis. In this work, we have explored the therapeutic potential of the kinetochore (KT) protein Hec1 (Highly Expressed in Cancer protein 1) as a molecular target to produce massive chromosome missegregation ...
Haque I I 1] Cancer Research Unit, V.A. Medical Center, Kansas City, MO, USA [2] Division of Hematology and Oncology, Department of Medicine, University of Kansas Medical Center, Kansas City, MO, - - 2014
The matricellular protein CCN5/WISP-2 represents a promising target in triple-negative breast cancer (TNBC) because treatment or induced activation of CCN5 in TNBC cells promotes cell growth arrest at the G0/G1 phase, reduces cell proliferation and delays tumor growth in the xenograft model. Our studies found that the p27(Kip1) tumor suppressor ...
García-Ocaña Adolfo - - 2014
Adult human pancreatic β cells are refractory to current therapeutic approaches to enhance proliferation. This reluctance to expand is problematic, especially for people with diabetes who lack sufficient numbers of functional insulin-producing β cells and could therefore benefit from therapies for β cell expansion. In this issue of the JCI, ...
Han Rong R College of Pharmaceutical Science, Soochow University, Suzhou 215123, - - 2014
Aim:Crotoxin (CrTX) is the primary toxin in South American rattlesnake (Crotalus durissus terrificus) venom, and exhibits antitumor and other pharmacological actions in vivo and in vitro. Here, we investigated the molecular mechanisms of the antitumor action of CrTX in human lung carcinoma cells in vitro.Methods:Human lung squamous carcinoma SK-MES-1 cells ...
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