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Ling Yong Y School of Pharmacy, Nantong University, Nantong, 226001, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, PR - - 2014
Novel series of FTS-diamine/phenylpropenoic acid hybrids were designed and synthesized. Their in vitro growth inhibitory assays showed that most compounds displayed strong anti-proliferation activity against seven cancer cells. Especially, the new hybrid 12f, by the conjugation of 10a with ferulic acid, could selectively suppress the proliferation of tumor cells and ...
Battistelli M M DiSTeVA, Campus Scientifico Enrico Mattei, Università degli Studi di Urbino Carlo Bo, Via Ca' le Suore 2, 61029, Urbino, PU, Italy, - - 2014
Chondrocyte death and loss of extracellular matrix are the central features in articular cartilage degeneration during osteoarthritis pathogenesis. Cartilage diseases and, in particular, osteoarthritis are widely correlated to apoptosis but, chondrocytes undergoing apoptosis "in vivo" more often display peculiar features that correspond to a distinct process of programmed cell death ...
Woodenberg Wynston Ray WR School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, 4001, South Africa, - - 2014
Plant cell walls are dynamic entities that may change with development, differ between plant species and tissue type and play an important role in responses to various stresses. In this regard, the present investigation employed immunocytochemistry to determine wall composition and possible changes during development of immature and mature embryos ...
Tazo Yuki Y Department of Pathology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0374, - - 2014
Alternative splicing variants of survivin have different biological roles on cell kinetics. Here, we focused on the effects of different variants, including wild type (wt), survivin-ΔEx3, and survivin-2B, on apoptosis and cell proliferation in endometrial carcinomas (Em Cas). Expression of survivin-wt, survivin-ΔEx3, and survivin-2B with reference to cell death and ...
Su Li-Ya LY Clinical Medicine Research Center of the Affiliated Hospital, Inner Mongolia Medical University, No. 1 Tongdao North Street, Hohhot, Inner Mongolia, 010050, - - 2014
Recently, we reported that anticancer bioactive peptide (ACBP), purified from goat spleens immunized with human gastric cancer extracts, significantly inhibited gastric cancer cells in vitro and gastric tumors in vivo via repressing cell growth and promoting apoptosis, making it a promising potential biological anticancer drug. However, it is not known ...
Xi Di D Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, - - 2014
The tri-hybrid peptide-LHP7 has the potent activity against Gram-positive and Gram-negative as well as fungi, but its mechanism of action has remained elusive. The effluences of LHP7 on the Staphylococcus aureus cell membrane and targets of intracellular action were investigated. LHP7 exhibited an inhibitory effect on the S. aureus growth, ...
Li Bin B Department of Paediatrics, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, JS, 226001, People's Republic of - - 2014
RAF kinase inhibitor protein (RKIP) is a negative regulator of the RAS-mitogen-activated protein kinase/extracellular signal-regulated kinase signaling cascade. We investigated the expression of RKIP in chronic myelogenous leukemia (CML) K562 cells and the effects of RKIP on the characteristics of K562 cells. The recombinant plasmid pcDNA3.1-RKIP was established and transfected ...
Canaud Guillaume G Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, - - 2014
For several decades, acute kidney injury (AKI) was generally considered a reversible process leading to complete kidney recovery if the individual survived the acute illness. Recent evidence from epidemiologic studies and animal models, however, have highlighted that AKI can lead to the development of fibrosis and facilitate the progression of ...
Bühligen Franziska F Helmholtz Centre for Environmental Research - UFZ, Department of Environmental Microbiology, Permoserstraße 15, 04318 Leipzig, - - 2014
Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the same brewing yeast strain but different ...
Lamberti Dante D Fondazione Pasteur Cenci Bolognetti, Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology Sapienza University of - - 2014
GDNF is a Sertoli-cell-derived factor that controls the balance between self-renewal and differentiation of the spermatogonial stem cells. Although research in recent years has concentrated on the impact of GDNF on target germ cells rather little attention has been paid to the molecular control of GDNF expression in Sertoli cells. ...
Zhao Jing J Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States; Vascular and Medical Institute, University of Pittsburgh, Pittsburgh, PA, United - - 2014
The lysophosphatidic acid receptor 1 (LPA1), a G-protein coupled receptor, regulates cell proliferation, migration, and cytokine release. Here, we investigate the molecular signature of LPA1 trafficking to the cell surface. The overexpressed LPA1 with a C-terminal V5 tag (LPA1-V5) is majorly expressed on the cell surface, while two deletion mutants ...
Nishimura Toshiya T Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, and Graduate School of Advanced Study, SOKENDAI, Okazaki, - - 2014
Vertebrate reproduction depends on the function of 2 distinct gametes, sperm and eggs, which develop in 2 different organs, the testis and the ovary. Testes and ovaries are composed of germ cells, supporting cells and interstitial cells. In this review, we describe the origin and the fate of these cell ...
Oliveira Pedro F PF CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, - - 2014
Otto Warburg observed that cancerous cells prefer fermentative instead of oxidative metabolism of glucose, although the former is in theory less efficient. Since Warburg's pioneering works, special attention has been given to this difference in cell metabolism. The Warburg effect has been implicated in cell transformation, immortalization, and proliferation during ...
Wang Fang F Department of Medical Genetics, Second Military Medical University, Shanghai, 200433, China; Center of Reproductive Medicine, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, - - 2014
Many protein-coding oncofetal genes are highly expressed in murine and human fetal liver and silenced in adult liver. The protein products of these hepatic oncofetal genes have been used as clinical markers for the recurrence of hepatocellular carcinoma (HCC) and as therapeutic targets for HCC. Herein, we examined the expression ...
Leblond Pierre P Pediatric oncology unit, Centre Oscar Lambret, Lille, - - 2014
The poor prognosis of children with high-grade glioma (HGG) and high-risk neuroblastoma, despite multidisciplinary therapeutic approaches, demands new treatments for these indications. F14512 is a topoisomerase II inhibitor containing a spermine moiety that facilitates selective uptake by tumor cells via the Polyamine Transport System (PTS) and increases topoisomerase II poisoning. ...
Kyjacova L L Department of Genome Integrity, Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech - - 2014
Fractionated ionizing radiation combined with surgery or hormone therapy represents the first-choice treatment for medium to high-risk localized prostate carcinoma. One of the main reasons for the failure of radiotherapy in prostate cancer is radioresistance and further dissemination of surviving cells. In this study, exposure of four metastasis-derived human prostate ...
Abeille F F Univ. Grenoble Alpes, F-38000 Grenoble, - - 2014
Microfluidic bioreactors are expected to impact cell therapy and biopharmaceutical production due to their ability to control cellular microenvironments. This work presents a novel approach for continuous cell culture in a microfluidic system. Microcarriers (i.e., microbeads) are used as growth support for anchorage-dependent mammalian cells. This approach eases the manipulation ...
Lin Shu-Ping - - 2014
In order to gain insight into how interfacial effects influence cell responses, chemically modified anodized TiO2 nanotubes (ATNs) were used to simultaneously investigate the effects of nanoscale substrate structure and angstrom-scale chemicals on cell morphological change and cell growth. Two small chemicals were used to modify the ATNs, namely, 3-aminopropyltrimethoxysilane ...
Zhu Zhen Z Bio Engineering Laboratory (BEL), Department of Biosystems Science and Engineering (D-BSSE), ETH Zürich, Mattenstrasse 26, 4058, Basel, Switzerland, - - 2014
We present a microfluidic device, which enables single cells to be reliably trapped and cultivated while simultaneously being monitored by means of multifrequency electrical impedance spectroscopy (EIS) in the frequency range of 10 kHz-10 MHz. Polystyrene beads were employed to characterize the EIS performance inside the microfluidic device. The results demonstrate that ...
Vu Lucas T LT Virginia Tech, Chemical Engineering, Blacksburg, Virginia, United States ; - - 2014
The migration of cells is a complex process that is dependent on the properties of the surrounding environment. In vivo, the extra-cellular environment is complex with a wide range of physical features, topographies and protein compositions. There have been a number of approaches to design substrates that can recapitulate the ...
Hirabayashi Ayumi A Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. - - 2014
A purpose-designed porphyrin-peptide hybrid effectively degraded amyloid β monomer and oligomers associated with Alzheimer's disease. Degradation was achieved using light irradiation in the absence of any additives and under neutral conditions. Moreover, the hybrid effectively neutralized the cytotoxicity of amyloid β in PC12 cells upon photoirradiation.
Khurana Varun V Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City , Kansas City, MO , USA - - 2014
Abstract Purpose: The main goal of this study is to investigate the existence of sodium-dependent vitamin C transport system (SVCT2) and to define time-dependent uptake mechanism and intracellular regulation of ascorbic acid (AA) in human corneal epithelial (HCEC) and human retinal pigment epithelial (D407) cells. Methods: Uptake of [(14)C] AA ...
Mooranian Armin A Biotechnology and Drug Development Research Laboratory, School of Pharmacy, CHIRI Biosciences Research Precinct, Curtin University , Perth, WA , - - 2014
Introduction: In a recent study, we confirmed good chemical and physical compatibility of microencapsulated pancreatic β-cells using a novel formulation of low viscosity sodium alginate (LVSA), Poly-L-Ornithine (PLO), and the tertiary bile acid, ursodeoxycholic acid (UDCA). This study aimed to investigate the effect of UDCA on the morphology, swelling, stability, ...
Kopsachilis Nikolaos N Moorfields Eye Hospital , London , UK - - 2014
Abstract Purpose: To investigate the toxic effect of air on primary human retinal pigment epithelium cells (RPE) over time. Methods: RPE cultures were retrieved from six donor eyes and cultivated at 37 °C in 5% CO(2) and 95% humidified air. The RPE were divided in six groups with each group containing ...
Xi Dongmei D Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China. - - 2014
A novel strategy was proposed for Ramos cell detection by combining the expression of enhanced green fluorescent protein (eGFP) with the cell aptamer recognition and the triplex molecular beacons. This system was successfully applied to cancer cell detection with high sensitivity and specificity.
Chen Yuk-Kwan YK 1] Department of Oral Pathology, Faculty of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan [2] Division of Dentistry, Department of Oral Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan [3] Oral and Maxillofacial Imaging Center, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, - - 2014
Previous studies have demonstrated that spheroid type cells grown under suspension culture conditions have cancer stem cell (CSC) traits in a number of cancers, but this phenomenon has not yet been reported in the VX2 rabbit oral cancer model. Hence, this study aimed to study the spheroid cells from VX2 ...
Guimarães Gustavo Narvaes GN Department of Morphology, Division of Histology and Embryology, Piracicaba Dental School, University of Campinas, Av. Limeira 901, Caixa Postal 052 CEP, Piracicaba, São Paulo, 13414-903, - - 2014
Parathyroid hormone (PTH) plays a key role in the development and homeostasis of mineralized tissues such as bone and dentine. We have reported that PTH (1-34) administration can increase dentine formation in mice and that this hormone modulates in vitro mineralization of odontoblast-like cells. The purpose of the present study ...
Jiang Jun J Department of Oncology, The Central Hospital of Wuhan, 430014, Wuhan, - - 2014
Non-small cell lung cancer (NSCLC) is the major cause of cancer death worldwide. Increasing evidence shows that microRNAs (miRNAs), evolutionally conserved non-coding RNAs, are widely involved in the development and progression of NSCLC. Aberrant alteration of miRNAs expression has been implicated in NSCLC initiation and progression. Herein, we studied the ...
Ma Rui - - 2014
In this study, a nano-calcium silicate (n-CS)/polyetheretherketone (PEEK) bioactive composite was prepared using a process of compounding and injection-molding. The mechanical properties, hydrophilicity and in vitro bioactivity of the composite, as well as the cellular responses of MC3T3-E1 cells (attachment, proliferation, spreading and differentiation) to the composite, were investigated. The ...
Kosourov Sergey S Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku, - - 2014
We have investigated two approaches to enhance and extend H2 photoproduction yields in heterocystous, N2-fixing cyanobacteria entrapped in thin alginate films. In the first approach, periodic CO2 supplementation was provided to alginate-entrapped, N-deprived cells. N-deprivation led to the inhibition of photosynthetic activity in vegetative cells and the attenuation of H2 ...
Pauls Eduardo E AIDS Research Institute-IrsiCaixa and AIDS Unit, Hospital Germans Trias i Pujol, Autonomous University of Barcelona, 08916 Badalona, - - 2014
Proliferating cells are preferentially susceptible to infection by retroviruses. Sterile α motif and HD domain-containing protein-1 (SAMHD1) is a recently described deoxynucleotide phosphohydrolase controlling the size of the intracellular deoxynucleotide triphosphate (dNTP) pool, a limiting factor for retroviral reverse transcription in noncycling cells. Proliferating (Ki67(+)) primary CD4(+) T cells or ...
Saito Katsuya K Department of Neurosurgery, Keio University School of Medicine, Tokyo, Japan (K.S., S.T., K.Y., M.T.); Neuro-immunology Research Group, Keio University School of Medicine, Tokyo, Japan (Y.I., S.O., M.T.); Department of Physiology, Keio University School of Medicine, Tokyo, Japan (S.O.); Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan (K.N., Y.K.); Division of Gene Regulation, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan - - 2014
A cDNA library made from 2 glioma cell lines, U87MG and T98G, was screened by serological identification of antigens by recombinant cDNA expression (SEREX) using serum from a glioblastoma patient. Elongation factor Tu GTP binding domain containing protein 1 (EFTUD1), which is required for ribosome biogenesis, was identified. A cancer ...
Seo Young Ah YA Harvard School of Public Health, United - - 2014
The zinc (Zn) transporter ZnT2 imports Zn into secretory vesicles to export Zn from the mammary epithelial cell. Mutations in ZnT2 substantially impair Zn secretion into milk and result in neonatal Zn deficiency. Basal levels of the lactogenic hormone prolactin (PRL) are high during lactation and transiently increase in response ...
Kanaji Nobuhiro N Kagawa - - 2014
Background: This study assessed the effect of extended exposure to cigarette smoke extract (CSE) on tissue repair functions in lung fibroblasts. Methods: Human fetal (HFL-1) and adult lung fibroblasts were exposed to CSE for 14 days. Senescence-associated β-galactosidase (SA β-gal) expression, cell proliferation and tissue repair functions including chemotaxis and ...
Renaudin Xavier - - 2014
The aim of this study was to identify novel substrates of the FANCcore complex, which inactivation leads to the genetic disorder Fanconi anemia (FA), which associates bone marrow failure, developmental abnormalities and predisposition to cancer. Eight FANC proteins participate in the nuclear FANCcore complex, an E3 ubiquitin-ligase that monoubiquitinates FANCD2 ...
Zhang Zhichao Z Department of Food Science and Engineering, Jinan University, Guangzhou 510632, People's Republic of China. tpxchun@jnu.edu.cn - - 2014
Part of quercetin is coerced into the colon after ingestion and interacts with the gut microbiota. The interaction between quercetin and gut microbiota will influence human health. The cytotoxicity of quercetin and its metabolites from human gut bacteria in vitro fermentation was investigated in this study. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) (MTT) and ...
Orekhov Alexander N AN Institute for Atherosclerosis Research, Skolkovo Innovative Center, Skolkovo, Moscow, - - 2014
Pericytes which are also known as Rouget cells or perivascular cells are considered to represent a likely distinct pool of vascular cells that are extremely branched and located mostly in the periphery of the vascular system. The family of pericytes is a heterogeneous cell population that includes pericytes and pericyte-like ...
Barile Lucio L Laboratory of Molecular and Cellular Cardiology, Departments of Cardiology and Heart Surgery, Fondazione Cardiocentro Ticino, Lugano, - - 2014
Recent evidence suggests cardiac progenitor cells (CPC) may improve cardiac function after injury. The underlying mechanisms are indirect, but their mediators remain unidentified. Exosomes and other secreted membrane vesicles, hereafter collectively referred to as extracellular vesicles (EVs), act as paracrine signalling mediators. Here we report that EVs secreted by human ...
Zhu Shunqin S Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, P.R. - - 2014
Artemisinin, a natural product from the Chinese medicinal plant, Artemisia annua L., is commonly used in the treatment of malaria, and has recently been reported to have potent anticancer activity in various types of human tumors. Yet, the effect of artemisinin on neuroblastoma is still unclear. In the present study, we ...
Moreno Eduardo E Institute of Cell Biology, IZB, University of Bern, Bern CH-3012, Switzerland. Electronic address: - - 2014
Metazoans have evolved ways to engage only the most appropriate cells for long-term tissue development and homeostasis. In many cases, competitive interactions have been shown to guide such cell selection events. In Drosophila, a process termed cell competition eliminates slow proliferating cells from growing epithelia. Recent studies show that cell ...
Cai Danfeng D Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Molecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, CA 93106-9625, - - 2014
The traditional view of directional cell migration within a tissue is that it requires a gradient of a soluble attractive chemical that is stable in space and time due to the presence of a source and a sink. However, advances in live imaging technology and the ability to study cell ...
Magalhães Ana Cathia AC Neuroscience Center, University of Helsinki, Viikinkaari 4, 00014 Helsinki, Finland. Electronic address: - - 2014
Immunohistochemistry (IHC) studies in paraffin-embedded sections can be challenging due to epitope masking. To counteract this problem the microwave oven is widely used for heat-induced epitope retrieval (HIER). However, with this method important parameters cannot be specifically controlled, such as the intensity and duration of heating. New method: We describe ...
Schwartz Benedikt B Max Planck Institute for Molecular Genetics, Department of Developmental Genetics, Ihnestrasse 63-73, 14195 Berlin, Germany; Free University Berlin, Dept. of Biology, Chemistry and Pharmacy, Takustrasse 3, 14195 Berlin, - - 2014
Mesoderm formation in the mouse embryo initiates around E6.5 at the primitive streak and continues until the end of axis extension at E12.5. It requires the process of epithelial-to-mesenchymal transition (EMT), wherein cells detach from the epithelium, adopt mesenchymal cell morphology, and gain competence to migrate. It was shown previously ...
Ojha Rani R Department of Urology, Post Graduate Institute of Medical Education and Research, Chandigarh, India, - - 2014
The mechanisms that underlie tumor formation and progression have not been elucidated in detail in cancer biology. Recently, the identification of a tumor cell subset defined as cancer stem cells (CSC), which is enriched for tumor initiating capacity, has engendered new perspectives toward selective targeting of tumors. In this study, ...
Kim Wook W Department of Molecular Science and Technology, Ajou University, Suwon 443-749, South - - 2014
Pancreatic polypeptide (PP) is a major agonist for neuropeptide Y4 receptors (NPY4R). While NPY4R has been identified in various tissues, the cells on which it is expressed and its function in those cells has not been clearly delineated. Here we report that NPY4R is present in all somatostatin-containing cells of ...
Han V Z VZ Center for Integrative Brain Research, Seattle Children's Research Institute Seattle, WA 98101. Electronic address: - - 2014
In the mammalian cerebellum, deep cerebellar nuclear (DCN) cells convey all information from cortical Purkinje cells (PCs) to premotor nuclei and other brain regions. However, how DCN cells integrate inhibitory input from PCs with excitatory inputs from other sources has been difficult to assess, in part due to the large ...
Beninati Simone S Department of Biology, University "Tor Vergata", Rome, Italy. Electronic address: - - 2014
In this study, we have evaluated the potential antineoplastic effects of α-mangostin (α-M), the most representative xanthone in Garcinia mangostana pericarp, on melanoma cell lines. This xanthone markedly inhibits the proliferation of high-metastatic B16-F10 melanoma cells. Furthermore, by deeply analyzing which steps in the metastatic process are influenced by xanthone ...
Fukui Yasuhisa Y Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan town, 35053, Miaoli County, Taiwan, ROC. Electronic address: - - 2014
Signet ring cell carcinomas are highly malignant dedifferentiated adenocarcinomas. There are no cell-cell interactions between these round-shaped cells. They contain huge numbers of vacuoles, filled with mucins, which are secreted from the cells. The mechanism behind this phenotype has recently begun to be elucidated. In highly differentiated adenocarcinomas the ErbB2/ErbB3 ...
Durzyńska Julia J Department of Molecular Virology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, 61‑614 Poznań, - - 2014
The human Igf-1 gene not only produces insulin‑like growth factor-I (IGF-I), but also different carboxy‑terminal extensions, known as E peptides, through alternative splicing. We and others have shown that human Eb peptide (hEb) derived from Igf-1 has intrinsic biological activity and is localized to nuclei of transfected cells. Since hEb ...
Lu Y F YF Department of Endocrinology, Tangshan Workers Hospital, Tangshan 063000, P.R. - - 2014
We determined the expression and function of B cell translocation gene 1 (BTG1) in thyroid carcinoma. Thyroid samples were obtained from cancer lesions (n=83) and adjacent normal tissue (n=35) in thyroid cancer patients immediately after endoscopic biopsy. BTG1 expression was determined by immunohistochemistry and western blotting. The effect of BTG1 ...
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