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Guo Yingshu - - 2012
A disulfide-bound molecular beacon (MB) is reported to respond sensitively to changing levels of glutathione in vitro. Importantly, this successful application of a MB has exciting potential for monitoring cellular thiol.
Welsh J - - 2012
Introduction:  Cancers are associated with varying degrees of an increased risk of venous thromboembotic events (VTE) occurring. This increased risk is tumour driven and associated with tumour expression of tissue factor (TF) and tumour-derived microparticles (MP). In this study, cancer cell lines from phenotypically distinct tumours were assessed for cell ...
Karplus P Andrew PA Department of Biochemistry and Biophysics, Oregon State University, 2011 Ag Life Sciences Building, Corvallis, OR 97331, - - 2012
In this issue of Molecular Cell, Day et al. (2012) reveal a surprising benefit of peroxiredoxin inactivation at high H(2)O(2), showing that in Schizosaccharomyces pombe turning off peroxide defenses preserves the pool of reduced thioredoxin for repairing proteins vital to survival.
Baik Jong Youn JY College of Nanoscale Science and Engineering, University at Albany-State University of New York, 257 Fuller Road, Albany, NY 12203, - - 2012
Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Chinese hamster ovary (CHO) cells, commonly used mammalian host cells for production of foreign pharmaceutical proteins in ...
Chu Hai-Bo - - 2012
The purpose of this study is to describe the infiltration of mast cells as well as T and B cells in the walls of thrombotic varicose great saphenous veins. Sections were obtained from venous segments of patients with varicose veins and stained with toluidine blue for mast cells, while immunohistochemistry ...
Fontes A M - - 2012
Hemophilia A is the most common X-linked bleeding disorder; it is caused by deficiency of coagulation factor VIII (FVIII). Replacement therapy with rFVIII produced from human cell line is a major goal for treating hemophilia patients. We prepared a full-length recombinant FVIII (FVIII-FL), using the pMFG-P140K retroviral vector. The IRES ...
Dudas B - - 2012
Glycosaminoglycans (GAGs) are basic building blocks of the ground substance of the extracellular matrix and present at the cellular level as an important component of the glycocalyx covering the cell membrane. In addition to the general role of GAGs in maintaining the integrity of the cell and extracellular matrix by ...
Orlova N A - - 2012
Prophylaxis and treatment of inherited clotting disorder hemophilia A requires regular administration of factor VIII. Recombinant factor VIII, which is produced in CHO or BHK cells, is equivalent to the plasma-derived one and is prevalent in current clinical practice in developed countries. Development of a biosimilar recombinant FVIII requires the ...
Lenaerts Liesbeth - - 2012
Application of human adenovirus type 5 (Ad5) derived vectors for cancer gene therapy has been limited by the poor cell surface expression, on some tumor cell types, of the primary Ad5 receptor, the coxsackie-adenovirus-receptor (CAR), as well as the accumulation of Ad5 in the liver following interaction with blood coagulation ...
Melo-Silveira Raniere Fagundes - - 2012
Xylan is one of most abundant polymer after cellulose. However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhanced by ...
Guzmán-Urióstegui Alberto - - 2011
Carpospore output and development in the marine red alga Hydropuntia cornea J. Agardh. were increased by adding polyamines (PAs) (putrescine, spermidine and spermine) singly or in combinations at 10(-9), 10(-6) and 10(-3) M. Cell divisions after spore release and development of apical axis between 17 and 21 days characterized carpospore development. PAs ...
Casini Paola - - 2011
Hyaluronan is a crucial glycosaminoglycan of the vertebrate embryonic extracellular matrix able to influence cell behaviour, both by assembling the pericellular matrices and by activating signal transducing receptors such as CD44. We showed that the hyaluronan synthases, Has1 and Has2, and CD44 display a dynamic expression pattern during cranial neural ...
Ma Nan - - 2011
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MRC-5 cells in modified SAGM (small airway growth medium). The BMSC-derived type II alveolar epithelial cells morphologically resemble ...
Lorenzini Antonello - - 2011
We have examined the tolerance of the spindle assembly checkpoint (SAC), as measured by the appearance of tetraploid cells in the presence of a microtubule inhibitor, in a series of primary cell strains derived from species with diverse lifespan and body size. We find that the integrity of the SAC ...
Patthey Cédric - - 2011
During early vertebrate development, the embryonic ectoderm becomes subdivided into neural, neural plate border (border) and epidermal regions. The nervous system is derived from the neural and border domains which, respectively, give rise to the central and peripheral nervous systems. To better understand the functional nervous system we need to ...
Tong Yiai - - 2011
Huntington's disease (HD), caused by an expanded triplet repeat in the huntingtin (Htt) gene, results in extensive neuropathology, but study of the Htt gene in CNS development through gene knockout is problematic as the knockout leads to embryonic lethality in mice. Here, we report that the knockdown of Htt expression ...
Ivec Martin - - 2011
OBJECTIVE: To determine the influence of delayed compaction and fragmentation on the developmental capacity of morulas. DESIGN: Prospective study. SETTING: University IVF center. PATIENT(S): Intracytoplasmic sperm injection (ICSI) cycles with compact embryos on day 4 or day 5. INTERVENTION(S): The embryos were divided into day 4 (n = 329) and ...
Topczewski Jacek - - 2011
Out of the several signaling pathways controlling craniofacial development, the role of planar cell polarity (PCP) signaling is relatively poorly understood. This pathway, originally identified as a mechanism to maintain cell polarity within the epithelial cells of the Drosophila wing, has been linked to the proper development of a wide ...
Bugeon Laurence L Imperial College London, London, SW7 2AZ, - - 2011
Controversy persists regarding the role of Notch signaling in myelopoiesis. We have used genetic approaches, employing two Notch zebrafish mutants deadly seven (DES) and beamter (BEA) with disrupted function of notch1a and deltaC, respectively, and Notch1a morphants to analyze the development of leukocyte populations in embryonic and mature fish. Myelomonocytes ...
Chawengsaksophak Kallayanee - - 2011
Disruptions in the regulatory pathways controlling sex determination and differentiation can cause disorders of sex development (DSD), often compromising reproductive function. Although extensive efforts have been channeled into elucidating the regulatory mechanisms controlling the many aspects of sexual differentiation, the majority of DSD phenotypes are still unexplained at the molecular ...
Ma Yan-Lei - - 2011
The increase in proliferation and the lack of differentiation of cancer cells resemble what occur in the embryonic stem cells during physiological process of embryogenesis. There are also striking similarities in the behaviour between the invasive placental cells and invasive cancer cells. In the present study, microarrays were used to ...
Moir L M - - 2011
Lymphangioleiomyomatosis (LAM) is characterized by the abnormal growth of smooth muscle-like cells (LAM cells) and cystic destruction of the lung parenchyma. LAM cell-derived matrix metalloproteinases (MMPs) are thought to play a prominent role in the tissue destruction. The aim of this study was to determine whether doxycycline, a known MMP ...
Rishniw Mark - - 2011
The following on molecular aspects of esophageal development contains commentaries on esophageal striated myogenesis and transdifferentiation; conversion from columnar into stratified squamous epithelium in the mouse esophagus; the roles for BMP signaling in the developing esophagus and forestomach; and evidence of a direct conversion from columnar to stratified squamous cells ...
Taleb Nadine N Departments of Pediatrics and Human Genetics, McGill University, Montreal, QC, Canada. - - 2011
RFX6 has recently been found to be essential for the development of the endocrine pancreas through studies in both humans and mice. Interestingly, this gene maintains a specific expression in the postnatal hormone producing cells of the pancreas. Moreover in humans, different types of diabetes mellitus affect obligate carriers of ...
Mo Wei-Chuan - - 2011
The hypogeomagnetic field (HGMF; magnetic fields <200 nT) is one of the fundamental environmental factors of space. However, the effect of HGMF exposure on living systems remains unclear. In this article, we examine the biological effects of HGMF on the embryonic development of Xenopus laevis (African clawed frog). A decrease in ...
Cuervo Patricia - - 2011
Protozoan parasites are responsible for an impressive disease burden in developing and less-developed countries. The development of vaccines and effective new therapies for dealing with these organisms are among the main gaps to be filled in the control of protozoan parasite diseases. Programmed cell death (PCD) pathways have gained attention ...
Pu Yunqiao - - 2011
Diminishing fossil fuel resources as well as growing environmental and energy security concerns, in parallel with growing demands on raw materials and energy, have intensified global efforts to utilize wood biopolymers as a renewable resource to produce biofuels and biomaterials. Wood is one of the most abundant biopolymer composites on ...
Gribbins Kevin - - 2011
Sceloporus bicanthalis is a viviparous lizard that lives at higher elevations in Mexico. Adult male S. bicanthalis were collected (n = 36) from the Nevado de Toluca, Mexico (elevation is 4200 m) during August to December, 2007 and January to July, 2008. Testes were extracted, fixed in Trumps, and dehydrated ...
Rao Mahendra S - - 2011
The cell therapy branch of the regenerative medicine field has been innovative in developing new models of delivery and development and identifying alternative sources of funding. We discuss the implications of these changes for pharmaceutical companies and the opportunities they offer to a new entrepreneur.
Yu Tian T Department of Craniofacial Development, King's College London, London, - - 2011
Fibroblast growth factors (FGFs) and regulators of the FGF signalling pathway are expressed in several cell types within the cerebellum throughout its development. Although much is known about the function of this pathway during the establishment of the cerebellar territory during early embryogenesis, the role of this pathway during later ...
Moawad Adel R - - 2011
Abstract The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocytes vitrified and thawed at the germinal vesicle (GV) stage, matured in vitro, and then ...
He Hong-Juan - - 2011
The programmed cell death (PCD) is important in maintaining the cell number homoeostasis of tissues and organs in metazoan. This process is regulated by the inhibitors of apoptosis proteins (IAPs). The function and mechanism of IAPs have been well studied in animal embryonic development and human cancers. However, the roles ...
Zhao Fan F Department of Chemistry, Brandeis University, Waltham, MA 02454, - - 2011
Here we report the first example of using β-galactosidase to trigger the formation of cell compatible, supramolecular nanofibers, which ultimately may lead to a new approach for the development of soft nanotechnology.
Polosukhin Vasiliy V - - 2011
Goblet cell hyperplasia is a common feature of chronic obstructive pulmonary disease (COPD) airways, but the mechanisms that underlie this epithelial remodelling in COPD are not understood. Based on our previous finding of hypoxia-inducible factor-1α (HIF-1α) nuclear localization in large airways from patients with COPD, we investigated whether hypoxia-inducible signalling ...
Maldonado C A - - 2011
Bronchiolar Clara cells are integral components of lung homeostasis, predominantly distributed in distal airways. In addition to the 16 kDa Clara cell protein, a major secretory product with anti-inflammatory effects, rat Clara cells express the glycan-binding protein galectin-3 and secrete it into the airways. Given the essential role of galectin-3 ...
Cheung Napo K M - - 2011
Embryos of aquatic animal model fish have proven to be useful organisms for developmental biology and for early life stage toxicity tests. By virtue of their transparent chorions, imaging of normal and abnormal development can be detected and related to exposure or to alterations due to environmental factors. However, the ...
Hamdaoui Nabila - - 2011
The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-dependent chloride channel in the plasma membrane of epithelia whose mutation is the cause of the genetic disease cystic fibrosis (CF). The most frequent CFTR mutation is deletion of Phe(508) and this mutant protein (delF508CFTR) does not readily translocate to the ...
Giangreco Adam - - 2011
Tracheal epithelial remodelling, excess mucus production, and submucosal gland hyperplasia are features of numerous lung diseases, yet their origins remain poorly understood. Previous studies have suggested that NF-κB signalling may regulate airway epithelial homeostasis. The purpose of this study was to determine whether deletion of the NF-κB signalling pathway protein ...
Suzuki Daniela E - - 2011
In the developing cerebellum, proliferation of granular neuroprogenitor (GNP) cells lasts until the early postnatal stages when terminal maturation of the cerebellar cortex occurs. GNPs are considered cell targets for neoplastic transformation, and disturbances in cerebellar GNP cell proliferation may contribute to the development of pediatric medulloblastoma. At the molecular ...
Pan Lin - - 2011
The let-7 family of microRNAs (miRNAs) are known to act as tumor suppressors and down-regulated in lung cancer. Recently, the RNA-binding protein Lin-28 was demonstrated to inhibit biogenesis of let-7 miRNAs by blocking both Drosha- and Dicer-mediated cleavage and accelerating decay of let-7 precursors. We selected NCI-H446 lung small cell ...
Roberge-White Emily - - 2011
Dictyostelium discoideum has served as a model for development and differentiation for over 70 years. Also regulated in Dictyostelium is the process of dedifferentiation, which consists of multiple cellular events that are separately regulated, providing an excellent model system for studying the return of partially differentiated cells to a more pluripotent ...
Moser Sandra C - - 2011
[This corrects the article on p. e1000451 in vol. 5.].
Wu Lingyan - - 2011
Peroxisome proliferator-activated receptor-γ (PPARγ) is an anti-inflammatory molecule. To assess its biological function in lung alveolar epithelial cells, a CCSP-rtTA/(tetO)(7)-dnPPARγ bitransgenic mouse model was generated. In this model, a dominant negative (dn) PPARγ-Flag fusion protein was overexpressed in lung alveolar type II (AT II) epithelial cells in an inducible manner ...
Eneling Kristina K Membrane Signaling Networks, Atherosclerosis Research Unit, Department of Medicine, CMM, Karolinska Institutet, Karolinska University Hospital-Solna, Stockholm, - - 2011
Salt-inducible kinase 1 (SIK1) in epithelial cells mediates the increases in active sodium transport (Na(+), K(+)-ATPase-mediated) in response to elevations in the intracellular concentration of sodium. In lung alveolar epithelial cells increases in active sodium transport in response to β-adrenergic stimulation increases pulmonary edema clearance. Therefore, we sought to determine ...
Feng Shipeng - - 2011
microRNAs play an important roles in cell growth, differentiation, proliferation and apoptosis. They can function either as tumor suppressors or oncogenes. We found that the overexpression of miR-192 inhibited cell proliferation in A549, H460 and 95D cells, and inhibited tumorigenesis in a nude mouse model. Both caspase-7 and the PARP ...
Park Je Won - - 2011
A new reduced hydroxamate, 2,3-dihydrotrichostatin A, was created from trichostatin A by employing a recombinant strain of Streptomyces venezuelae as a microbial catalyst. Compared with trichostatin A, 2,3-dihydrotrichostatin A showed similar antifungal activity against Saccharomyces cerevisiae, but, interestingly, approximately twice the cytostatic activity against human small-cell lung cancer cells. The ...
Marmai Cecilia C Department of Medicine and Cardiovascular Research Institute, University of California, San Francisco, California 94143-0111, - - 2011
Prior work has shown that transforming growth factor-β (TGF-β) can mediate transition of alveolar type II cells into mesenchymal cells in mice. Evidence this occurs in humans is limited to immunohistochemical studies colocalizing epithelial and mesenchymal proteins in sections of fibrotic lungs. To acquire further evidence that epithelial-to-mesenchymal transition occurs ...
Hsu Yu-Chih YC Department of Biochemistry, Developmental Genomics Group, Center of Excellence in Bioinformatics and Life Science, State University of New York at Buffalo, 701 Ellicott St. Buffalo, NY 14203, - - 2011
The Nuclear factor I (NFI) transcription factor family consists of four genes (Nfia, Nfib, Nfic and Nfix) that regulate the development of multiple organ systems in mice and humans. Nfib is expressed in both lung mesenchyme and epithelium and mice lacking Nfib have severe lung maturation defects and die at ...
Morello V - - 2011
Lung cancer is the leading cause of cancer death worldwide. The epidermal growth factor receptor (EGFR) represents the main target for non-small cell lung cancer (NSCLC) therapy, as its overexpression or constitutive activation contributes to malignancy and correlates with poor prognosis. Our previous work demonstrated that in epithelial cells β1 ...
Kuo Curtis - - 2011
Airway remodelling which includes increases in extracellular matrix (ECM) is characteristic feature of asthma, and is correlated to disease severity. Rhinovirus infections are associated with increased risk of asthma development in young children and are the most common cause of asthma exacerbations. In this paper we examined whether viral infections ...
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