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Gruenbaum Yosef Y Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem Givat Ram, Jerusalem 91904 - - 2014
In humans the superfamily of intermediate filament (IF) proteins is encoded by more than 70 different genes, which are expressed in a cell- and tissue-specific manner. IFs assemble into approximately 10 nm-wide filaments that account for the principal structural elements at the nuclear periphery, nucleoplasm, and cytoplasm. They are also ...
Marc Robert E RE Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah School of Medicine Salt Lake City, UT, - - 2014
The mammalian AII retinal amacrine cell is a narrow-field, multistratified glycinergic neuron best known for its role in collecting scotopic signals from rod bipolar cells and distributing them to ON and OFF cone pathways in a crossover network via a combination of inhibitory synapses and heterocellular AII::ON cone bipolar cell ...
Racioppi Claudia C Cellular and Developmental Biology Laboratory, Stazione Zoologica Anton Dorhn, Villa Comunale, 80121 Napoli, - - 2014
During the development of the central nervous system (CNS), combinations of transcription factors and signalling molecules orchestrate patterning, specification and differentiation of neural cell types. In vertebrates, three types of melanin-containing pigment cells, exert a variety of functional roles including visual perception. Here we analysed the mechanisms underlying pigment cell ...
McCall Jennifer R JR UNCW Center for Marine Science, 5600 Marvin K Moss Lane, Wilmington, NC 28409, USA. - - 2014
Brevetoxins are a family of ladder-framed polyether toxins produced during blooms of the marine dinoflagellate, Karenia brevis. Consumption of shellfish or finfish exposed to brevetoxins can lead to the development of neurotoxic shellfish poisoning. The toxic effects of brevetoxins are believed to be due to the activation of voltage-sensitive sodium ...
Smedler Erik E Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet Stockholm, - - 2014
Multicellular organisms rely on intercellular communication to regulate important cellular processes critical to life. To further our understanding of those processes there is a need to scrutinize dynamical signaling events and their functions in both cells and organisms. Here, we report a method and provide MATLAB code that analyzes time-lapse ...
Ishikawa Takahiro T Motor Disorders Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo, - - 2014
The cerebellum generates its vast amount of output to the cerebral cortex through the dentate nucleus (DN) that is essential for precise limb movements in primates. Nuclear cells in DN generate burst activity prior to limb movement, and inactivation of DN results in cerebellar ataxia. The question is how DN ...
Roet Kasper C D KC Laboratory for Neuroregeneration, Netherlands Institute for Neuroscience, an Institute of The Royal Academy of Arts and Sciences, Amsterdam, The - - 2014
In their natural habitat, the peripheral nerve, Schwann cells (SCs) form nicely aligned pathways (also known as the bands of Büngner) that guide regenerating axons to their targets. Schwann cells that are implanted in the lesioned spinal cord fail to align in pathways that could support axon growth but form ...
Pinto-Teixeira Filipe F Center for Genomics and Systems Biology, New York University Abu Dhabi, P.O. Box 129188, Abu Dhabi, United Arab - - 2014
Asymmetric division of neural progenitor cells is a crucial event in the generation of neuronal diversity and involves the segregation of distinct proteins into daughter cells, thereby promoting unique differentiation programs. Although it was known that Notch signaling acts postmitotically to orchestrate differentiation of daughter cells from asymmetrically dividing precursor ...
La Noce M M Dipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia, Secondo Ateneo di Napoli, 5 via L. Armanni, 80138 Napoli, - - 2014
Neural crest cells, delaminating from the neural tube during migration, undergo an epithelial-mesenchymal transition and differentiate into several cell types strongly reinforcing the mesoderm of the craniofacial body area - giving rise to bone, cartilage and other tissues and cells of this human body area. Recent studies on craniomaxillofacial neural ...
Quiñones-Valles César C Engineering and Biomedical Physics Department, Center for Research and Advanced Studies of the National Polytechnic Institute at Monterrey, Apodaca, Nuevo León, México; Genetic Engineering Department, Center for Research and Advanced Studies of the National Polytechnic Institute at Irapuato, Irapuato, Guanajuato, - - 2014
The division of Caulobacter crescentus, a model organism for studying cell cycle and differentiation in bacteria, generates two cell types: swarmer and stalked. To complete its cycle, C. crescentus must first differentiate from the swarmer to the stalked phenotype. An important regulator involved in this process is CtrA, which operates ...
Cai Ming M Shanghai Key Laboratory of Human Sport Competence Development and Maintenance, Shanghai University of sport, Shanghai, - - 2014
To investigate the effects of hypoxic conditioned media from rat cerebral cortical cells on the proliferation and differentiation of neural stem cells (NSCs) in vitro, and to study the roles of PI3-K/Akt and JNK signal transduction pathways in these processes. Cerebral cortical cells from neonatal Sprague-Dawley rat were cultured under ...
Collart-Dutilleul Pierre-Yves PY BioNano Laboratory EA 4203, Université Montpellier 1, Montpellier 34193, - - 2014
The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at their leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are the primary area within the cell of actin microfilaments (filopodia) formation. In this work, we report on the ...
Lowdon Rebecca F RF Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University in St Louis, St Louis, Missouri 63108, - - 2014
Developmental history shapes the epigenome and biological function of differentiated cells. Epigenomic patterns have been broadly attributed to the three embryonic germ layers. Here we investigate how developmental origin influences epigenomes. We compare key epigenomes of cell types derived from surface ectoderm (SE), including keratinocytes and breast luminal and myoepithelial ...
Zong Haibin H Functional Laboratory, School of Basic Medical Sciences, Xinxiang Medical College, Xinxiang 453003, Henan Province, - - 2013
Schwann cells and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposomes. Results showed that neurotrophin-3 was ...
Boubriak Olga A - - 2013
The intervertebral disc is an avascular tissue, maintained by a small population of cells that obtain nutrients mainly by diffusion from capillaries at the disc-vertebral body interface. Loss of this nutrient supply is thought to lead to disc degeneration, but how nutrient supply influences viable cell density is unclear. We ...
Fu Chunhua C Department of Surgery, University of Florida College of Medicine, Gainesville, FL, - - 2012
To evaluate direct versus indirect monocyte chemoattractant protein (MCP)-1/CCR2 signaling and to identify the cellular producers and effectors for MCP-1 during neointimal hyperplasia (NIH) development in vein grafts. Genomic analysis revealed an overrepresentation of 13 inflammatory pathways in wild-type vein grafts compared with CCR2 knockout vein grafts. Further investigation with ...
Bailey Caleb M - - 2012
Cancer cells must regulate plasticity and invasion to survive and metastasize. However, the identification of targetable mechanisms to inhibit metastasis has been slow. Signaling programs that drive stem and progenitor cells during normal development offer an inroad to discover mechanisms common to metastasis. Using a chick embryo transplant model, we ...
Gigi Roy - - 2012
Thromboprophylaxis reduces the risk of surgery-related deep vein thrombosis, but anticoagulants were associated with systemic osteoporosis, a known risk factor for poor fracture healing. Rivaroxaban (XARELTO®) is a novel anticoagulant with specific ability to inhibit factor Xa, a serine endopeptidase, which plays a key role in coagulation. The present study ...
Plochmann Kathrin - - 2012
The cellular receptor of foamy viruses (FVs) is unknown. The broad spectrum of permissive cells suggests that the cellular receptor is a molecular structure with almost ubiquitous prevalence. Here, we investigated the ability of heparan sulfate (HS), a glycosaminoglycan (GAG) present on the extracellular matrix of many cells, to bind ...
O'Keefe David D DD Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, - - 2012
Mechanisms that govern cell-fate specification within developing epithelia have been intensely investigated, with many of the critical intercellular signaling pathways identified, and well characterized. Much less is known, however, about downstream events that drive the morphological differentiation of these cells, once their fate has been determined. In the Drosophila wing-blade ...
Welzel Petra B - - 2012
Macroporous scaffolds with adaptable mechanical and biomolecular properties can be instrumental in enabling cell-based therapies. To meet these requirements, a cryostructuration method was adapted to prepare spongy hydrogels based on chemically cross-linked star-shaped poly(ethylene glycol) (starPEG) and heparin. Subzero temperature treatment of the gel forming reaction mixtures and subsequent lyophilization ...
Kurokawa Suguru - - 2012
In vivo studies have shown that selenium is supplied to testis and brain by apoER2-mediated endocytosis of Sepp1. Although cultured cell lines have been shown to utilize selenium from Sepp1 added to the medium, the mechanism of uptake and utilization has not been characterized. Rat L8 myoblast cells were studied. ...
Baik Jong Youn - - 2012
Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Since Chinese hamster ovary (CHO) cells are capable of producing heparan sulfate (HS), a related polysaccharide naturally, ...
Smillie I R A - - 2012
There is a need to develop rice plants with improved photosynthetic capacity and efficiency in order to enhance potential grain yield. Alterations in internal leaf morphology may be needed to underpin some of these improvements. One target is the production of a 'Kranz-like' anatomy, commonly considered to be required to ...
Walensky Loren D LD Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA. - - 2012
In this issue of Molecular Cell, Dumitru et al. (2012) report that hES cells localize a conformationally activated form of proapoptotic BAX to the trans Golgi network, a previously unanticipated launch pad for mitochondrial assault in response to DNA damage.
Pan Yan Y State Key Laboratory of Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing, - - 2012
To investigate the inhibitory effects of heparin on PC-3M cells proliferation in vitro and B16-F10-luc-G5 cells metastasis in Balb/c nude mice and identify the protein expression patterns to elucidate the action mechanism of heparin. Human prostate cancer PC-3M cells were incubated with heparin 0.5 to 125 μg/mL for 24 h. ...
Puvirajesinghe Tania M TM Centre for Glycobiology, Department of Biochemistry and Cell Biology, Institute of Integrative Biology, The University of Liverpool, Liverpool L69 7ZB, - - 2012
Array methodologies have become powerful tools for interrogation of glycan-protein interactions but have critically lacked the ability to generate cell response data. Here, we report the development of a slide-based array method exemplified by measurement of activation of fibroblast growth factor signaling by heparin saccharides. Heparan sulfate-deficient Swiss 3T3 cells ...
Kuo Yung-Chih - - 2012
The potential of constructs comprising induced pluripotent stem (iPS) cells and biopolymers can be high for neurological surgery practice, if the systematic activity of neuronal regeneration is clarified. This study shows a guided differentiation of iPS cells toward neurons in neuron growth factor (NGF)-grafted poly(ε-caprolactone) (PCL)-poly(β-hydroxybutyrate) (PHB) scaffolds. The porosity ...
Kang Dong Gyun - - 2012
Periplasmic secretion has been used in attempts to construct an efficient whole-cell biocatalyst with greatly reduced diffusion limitations. Previously, we developed recombinant Escherichia coli that expresses organophosphorus hydrolase (OPH) in the periplasmic space using the twin-arginine translocation (Tat) pathway to degrade environmental toxic organophosphate compounds (OPs). This system has the ...
Suga Naoko - - 2012
The function of CD44-v3 and heparin/heparan sulfate (HS) signaling was investigated during trophoblast cell migration to identify their role in the renewal of syncytial layer damage caused by increased hemodynamic turbulence in the intervillous space and maintenance of syncytial integrity in pre-eclampsia. We evaluated the effect of heparin/HS/CD44-v3-mediated processes during ...
Lee Sung-Bau - - 2012
In the current issue of Molecular Cell, Yu et al. (2012) establish H3K56 monomethylation (H3K56me1) as a new mammalian chromatin mark, imposed by the G9a methyltransferase and recognized by the replication clamp PCNA.
da Rosa Nathalia Gonsales - - 2012
Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant B-domain-deleted FVIII (rBDDFVIII) by the human cell line SK-HEP-1, modified by a lentiviral vector ...
Meng Shengnan - - 2012
Flavonoids are polyphenolic compounds with various claimed health benefits, but the extensive metabolism by uridine-5'-diphospho-glucuronosyltransferases (UGTs) and sulfotransferases (SULTs) in liver and intestine led to poor oral bioavailabilities. The effects of structural changes on the sulfonation of flavonoids have not been systemically determined, although relevant effects of structural changes on ...
Li Zhigang - - 2012
There are few reports that describe the physiological changes in a vein graft used to reconstruct lacrimal canaliculus. This article aims to observe the phasic changes in cell proliferation in an autogenous vein graft used to repair the lacrimal canaliculus. Lacrimal canaliculus disjunction was made in a rabbit model by ...
Ruf Wolfram W Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. - - 2012
The generation of procoagulant Tissue Factor (TF) is crucial for thrombosis. TF contains a surface exposed allosteric disulfide bond that stabilizes the carboxyl-terminal domain involved in ligand interactions with coagulation factors VIIa and X. TF procoagulant activation typically occurs following cellular perturbations that also cause the appearance of procoagulant phosphatidylserine ...
Takahashi Azusa - - 2012
Epithelial cells are pivotal in the separation of the body from the outside environment. Orally administered drugs must pass across epithelial cell sheets, and most pathological organisms invade the body through epithelial cells. Tight junctions (TJs) are sealing complexes between adjacent epithelial cells. Modulation of TJ components is a potent ...
Chitnis Ajay B - - 2012
The posterior lateral line (pLL) in zebrafish has emerged as an excellent system to study how a sensory organ system develops. Here we review recent studies that illustrate how interactions between multiple signaling pathways coordinate cell fate,morphogenesis, and collective migration of cells in the posterior lateral line primordium. These studies ...
Thu Mya S MS Frank Laboratory and Laboratory of Diagnostic Radiology Research, Department of Radiology and Imaging Sciences, US National Institutes of Health, Bethesda, Maryland, - - 2012
We report on a new straightforward magnetic cell-labeling approach that combines three US Food and Drug Administration (FDA)-approved drugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling nanocomplexes that effectively label cells for in vivo magnetic resonance imaging (MRI). We observed that the ferumoxytol-heparin-protamine (HPF) nanocomplexes were stable in serum-free ...
Fischer Sarah - - 2012
BACKGROUND: Implantation of silicone materials like iris diaphragms into the eye can be complicated by cell migration and attachment. We studied polydimethylsiloxane (PDMS) foils coated with isocyanate terminated, star-shaped poly(ethylene glycol-stat-propylene glycol) (NCO-sP(EO-stat-PO)) equipped with heparin towards the inhibition of cell attachment without influencing cell viability. METHODS: Mouse fibroblasts L929 ...
Guo Yingshu - - 2012
A disulfide-bound molecular beacon (MB) is reported to respond sensitively to changing levels of glutathione in vitro. Importantly, this successful application of a MB has exciting potential for monitoring cellular thiol.
Welsh J - - 2012
Introduction:  Cancers are associated with varying degrees of an increased risk of venous thromboembotic events (VTE) occurring. This increased risk is tumour driven and associated with tumour expression of tissue factor (TF) and tumour-derived microparticles (MP). In this study, cancer cell lines from phenotypically distinct tumours were assessed for cell ...
Karplus P Andrew PA Department of Biochemistry and Biophysics, Oregon State University, 2011 Ag Life Sciences Building, Corvallis, OR 97331, - - 2012
In this issue of Molecular Cell, Day et al. (2012) reveal a surprising benefit of peroxiredoxin inactivation at high H(2)O(2), showing that in Schizosaccharomyces pombe turning off peroxide defenses preserves the pool of reduced thioredoxin for repairing proteins vital to survival.
Baik Jong Youn JY College of Nanoscale Science and Engineering, University at Albany-State University of New York, 257 Fuller Road, Albany, NY 12203, - - 2012
Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Chinese hamster ovary (CHO) cells, commonly used mammalian host cells for production of foreign pharmaceutical proteins in ...
Chu Hai-Bo - - 2012
The purpose of this study is to describe the infiltration of mast cells as well as T and B cells in the walls of thrombotic varicose great saphenous veins. Sections were obtained from venous segments of patients with varicose veins and stained with toluidine blue for mast cells, while immunohistochemistry ...
Fontes A M - - 2012
Hemophilia A is the most common X-linked bleeding disorder; it is caused by deficiency of coagulation factor VIII (FVIII). Replacement therapy with rFVIII produced from human cell line is a major goal for treating hemophilia patients. We prepared a full-length recombinant FVIII (FVIII-FL), using the pMFG-P140K retroviral vector. The IRES ...
Dudas B - - 2012
Glycosaminoglycans (GAGs) are basic building blocks of the ground substance of the extracellular matrix and present at the cellular level as an important component of the glycocalyx covering the cell membrane. In addition to the general role of GAGs in maintaining the integrity of the cell and extracellular matrix by ...
Orlova N A - - 2012
Prophylaxis and treatment of inherited clotting disorder hemophilia A requires regular administration of factor VIII. Recombinant factor VIII, which is produced in CHO or BHK cells, is equivalent to the plasma-derived one and is prevalent in current clinical practice in developed countries. Development of a biosimilar recombinant FVIII requires the ...
Lenaerts Liesbeth - - 2012
Application of human adenovirus type 5 (Ad5) derived vectors for cancer gene therapy has been limited by the poor cell surface expression, on some tumor cell types, of the primary Ad5 receptor, the coxsackie-adenovirus-receptor (CAR), as well as the accumulation of Ad5 in the liver following interaction with blood coagulation ...
Melo-Silveira Raniere Fagundes - - 2012
Xylan is one of most abundant polymer after cellulose. However, its potential has yet to be completely recognized. Corn cobs contain a considerable reservoir of xylan. The aim of this work was to study some of the biological activities of xylan obtained from corn cobs after alkaline extraction enhanced by ...
Guzmán-Urióstegui Alberto - - 2011
Carpospore output and development in the marine red alga Hydropuntia cornea J. Agardh. were increased by adding polyamines (PAs) (putrescine, spermidine and spermine) singly or in combinations at 10(-9), 10(-6) and 10(-3) M. Cell divisions after spore release and development of apical axis between 17 and 21 days characterized carpospore development. PAs ...
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