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Deng Huiling H Department of Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. - - 2014
Hypoxia is a primary mediator for cell survival, and has been reported to inhibit cardiomyocyte proliferation in fetal and neonatal hearts. CyclinA2 is a key regulator of cell proliferation. Whether CyclinA2 affects cardiomyocyte proliferation in hypoxic conditions remains unexamined. This study was designed to investigate the roles of CyclinA2 expression ...
Shen Jian J Department of Cardiothoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. - - 2014
The aim of the present study was to investigate cobra neurotoxin (cobrotoxin) activity in A549 cell lines transplanted into nude mice, and to explore its molecular mechanism. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the growth inhibition rate of cobrotoxin in human lung A549 adenocarcinoma cells and HFL1 ...
Braunger Barbara M BM Institute of Human Anatomy and Embryology, University of Regensburg, Universitaetsstr. 31, Regensburg, D-93053, - - 2014
Purpose: To identify stem cells in the chamber angle of the monkey eye by detection of 5-bromo-2'-deoxyuridine (BrdU) long-term retention. Methods: Four cynomolgus monkeys were treated 4 weeks with BrdU via subcutaneous pumps. The eyes of two animals were processed immediately thereafter (group 1) while in the other animals BrdU ...
Yan Yulan Y Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. - - 2014
Newcastle disease virus (NDV) is a member of the genus Avulavirus in the Paramyxoviridae family and its antitumor properties depend on its ability to kill malignant cells while not affecting normal cells. The present study investigated a recombinant avirulent NDV LaSota strain (wild‑type NDV strain) expressing the rabies virus glycoprotein (rL‑RVG), examined ...
Li Xuezhen X Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. - - 2014
Notch signaling has been reported to be oncogenic or tumor suppressive, depending on the tissue context. To investigate the effects of Notch2 knockdown on U87 human glioma cell proliferation in vitro and in vivo, and the associated mechanisms, U87 cells were stably transfected with p green fluorescent protein (GFP)‑V‑RS Notch2 short ...
Yamasaki Shinya S Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, Osaka, - - 2014
The purpose of this study was to assess how peripheral blood cells (PBCs) contribute to meniscus repair, using a parabiotic rat model. Wild-type (WT) and green fluorescent protein (GFP) transgenic rats were conjoined at the torso. After 4 weeks, the anterior part of the medial meniscus of both groups of ...
Fick J M JM Department of Applied Physics, University of Eastern Finland, Kuopio FI-70211, Finland. Electronic address: - - 2014
To determine if increasing cartilage cross-links through in vitro glycation of cartilage explants can alter the biomechanical response of chondrocytes to compressive deformation. Bovine osteochondral explants were either incubated with cell culture solution supplemented with (n = 7) or without (n = 7) ribose for 42 h in order to induce glycation. Deformation-induced changes in cell volume, ...
Belal Mahmoud Helmy MH 1 Department of Oral Medicine, Periodontology & Oral Diagnosis, Faculty of Oral & Dental Medicine, Fayoum University , Bahaariy Region, Al-Fayoum, Egypt - - 2014
Abstract Objective: Clinical application of lasers in periodontal therapy has continued to expand in last decades; however there are still some controversies. The present study aimed to compare the conditioning effects of the carbon dioxide (CO2) or erbium-doped: yttrium, aluminum and garnet (Er:YAG) laser on periodontally diseased root surfaces following ...
Sakagami Hiroshi H Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University, School of Dentistry, - - 2014
The present article reviewed the biological activities and possible dental application of three major polyphenols, i.e., lignin-carbohydrate complexes, tannins, and flavonoids, citing mostly our in vitro studies together with those from other groups. All these polyphenols showed much lower tumor-selective cytotoxicity against oral squamous cell carcinoma cells vs. normal oral ...
Peng Cheng C Department of Urology, 456 Hospital of PLA, No. 25 Wuyingshan Road, Jinan, 250031, - - 2014
Multiple studies have shown that CC motif chemokine ligand 19 (CCL19) promotes cell proliferation in several human cancers. In this study, we investigated the clinical significance of CCL19 and its specific receptor CCR7 and its function in our large collection of prostate samples. Between August 2000 and December 2013, 108 ...
Chen Lei L 1] State Key Laboratory of Palaeobiology and Stratigraphy, and Key Laboratory of Economic Stratigraphy and Palaeogeography, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, China [2] College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, - - 2014
Phosphorites of the Ediacaran Doushantuo Formation (∼600 million years old) yield spheroidal microfossils with a palintomic cell cleavage pattern. These fossils have been variously interpreted as sulphur-oxidizing bacteria, unicellular protists, mesomycetozoean-like holozoans, green algae akin to Volvox, and blastula embryos of early metazoans or bilaterian animals. However, their complete life cycle ...
Rosseti Isabela Bueno - - 2014
Candida species are the fourth most common cause of nosocomial bloodstream infections. It has been observed an increase in the frequency of infections which have become refractory to standard antifungal therapy. Recently, it was demonstrated the effect of different organochalcogenide compounds reducing both growth and germ tube formation by Candida ...
Hussein Shaymaa Subhi SS Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, - - 2014
Mosaicism is present in more than 50% of the cases with small supernumerary marker chromosomes (sSMCs) and karyotype 47,XX,+mar/46,XX or 47,XY,+mar/46,XY. Recently we provided first evidence that the mitotic stability of sSMC is dependent on their structure, i.e. their shape. Thus, here we performed a long term in vitro study ...
Navarini Natalia Festugatto NF Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua José Rocha Junqueira, 13, 13045-610, Campinas, São Paulo, Brazil, - - 2014
During tumor development, benign neoplastic cells are influenced by the expression of cytokines, growth factors, and proteases present in the tumor microenvironment. Epidermal growth factor (EGF) is the most studied growth factor and is considered important for cell proliferation and migration. Metalloproteinases (MMPs) are also involved in tumor progression. The ...
Chen Li L Department of Toxicology, School of Public Health, Wuhan University, Wuhan, Hubei 430071, P.R. - - 2014
This study was designed to investigate the direct effects of fatty acids (FAs) on the cell viability and the expression levels of genes involved in lipid metabolism in LO2 human liver cells. Palmitate (PA), oleate (OA) and docosahaexenoic acid (DHA) were used to represent saturated, mono-unsaturated and polyunsaturated FAs, respectively. ...
Centola Matteo M University Hospital Basel, Departments of Surgery and of Biomedicine, Basel, Switzerland ; - - 2014
Enzymatic isolation of chondrocytes from a cartilage biopsy is the first step to establish in vitro models of chondrogenesis or to generate cell-based grafts for cartilage repair. Such process is based on manually operated procedures and typically results in yields lower than 20% of the total available cells. In this ...
Tsuyama Naohiro N Department of Radiation Life Sciences, Fukushima Medical University, 1 Hikarigaoka, Fukushima-shi, Fukushima 960-1295, Japan - - 2014
Ionizing radiation (IR) induces cellular stress responses, such as signal transduction, gene expression, protein modification, and metabolite change that affect cellular behavior. We analyzed X-irradiated human Epstein-Barr virus-transformed B lymphoblastoid cells and normal fibroblasts to search for metabolites that would be suitable IR-responsive markers by Liquid Chromotography-Mass spectrometry (LC-MS). Mass ...
Zhang Zhuoli Z From the Department of Radiology (Z.Z., W.L., D.P., K.L., A.Y.S., A.C.G., Y.G., A.C.L.), Robert H. Lurie Comprehensive Cancer Center (Z.Z., K.K., A.C.L.), and Department of Biomedical Engineering (A.C.L.), Northwestern University, 737 N Michigan Ave, 16th Floor, Chicago, IL 60611; Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (Z.Z., Y.H., A.C.L.); and Department of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, China - - 2014
Purpose To test the following hypotheses in a murine model of pancreatic cancer: (a) Vaccination with antigen-loaded iron-labeled dendritic cells reduces T2-weighted signal intensity at magnetic resonance (MR) imaging within peripheral draining lymph nodes (LNs) and (b) such signal intensity reductions are associated with tumor size changes after dendritic cell ...
Erichsen Julie Ladeby JL Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, - - 2014
Human neural stem cells (NSCs) from the developing embryo or the subventricular zone of the adult brain can potentially elicit brain repair after injury or disease, either via endogenous cell proliferation or by cell transplantation. Profound knowledge of the diverse signals affecting these cells is, however, needed to realise their ...
Zhao Hongmin H Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University , Harbin, People's Republic of China - - 2014
Cisplatin is a most active drug for the treatment of ovarian cancer; however, acquired cisplatin resistance is easily seen in patients with ovarian cancer. The aim of this study is to clarify the molecular mechanism of cisplatin resistance and try to reverse cisplatin resistance in ovarian cancer lines in vitro ...
Ma Yongjiang Y Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, P.R. - - 2014
Piperine is an important active component of the Chinese herb Large leaf moss. The aim of this study was to investigate the effects of piperine on oxidative stress. An oxidative stress model was developed in rabbit atrial cells treated with low concentrations of hydrogen peroxide (H2O2). A primary cell culture ...
Wang Benquan B Department of Surgery The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. - - 2014
Drug resistance is a major impediment to successful chemotherapy in pancreatic cancer (PC) patients. We investigated the effect of Wnt/β-catenin signaling inhibition by wnt-c59 on chemoresistance in a trichostatin A-resistant Panc-1 cell line (Panc-1/TSA). Panc-1/TSA cells were treated with the Wnt/β‑catenin signaling inhibitor wnt-c59 (10 µmol · l-1) and/or trichostatin A (TSA; 10 µmol · l-1) for ...
Mahaldashtian Maryam M a Department of Molecular & Cellular Biology, Faculty of Biology , Damghan University , Semnan , - - 2014
There is a fast growing tendency in the use of herbal remedies in developing countries. One of the traditional medicines used for male infertility treatment is date palm (Phoenix dactylifera) pollen (DPP). Isolated spermatogonial stem cells and sertoli cells using enzymatic digestion were grown in Dulbecco's modified Eagle's medium supplemented ...
Habibi B B Department of Physiology, School of Medicine, Tehran University of Medical Science, Poursina Ave, Tehran, Islamic Republic of Iran, - - 2014
Pre-pubertal varicocele can result in hypotrophy of testes, progressive deterioration of Sertoli cells and spermatogonia cell number, decrease in seminiferous diameter and cause to sperm damage. Because of detrimental time-dependent effects of varicocele, this study describes the effects of varicocele on the levels of interleukin-6 (IL-6) and interferon-gamma in serum ...
Tian Yuanhu Y Department of Hepatobiliary Surgery, Chinese People's Liberation Army General Hospital, Beijing 100853, China; Department of General Surgery, Peking University Third Hospital, Beijing 100191, - - 2014
The possible role of substance P (SP) during wound healing has been the primary research focus in recent years, but its effect on the healing process after bile duct injury is little understood. This study aimed to investigate the effects of SP on growth of fibroblast-like cells derived from rabbit ...
Kou Wei W The School of Basic Medical Sciences, Lanzhou University, Lanzhou, China. E-mail. - - 2014
To investigate the radiosensitizing effects of Radix Angelicae Sinensis-Radix Hedysari (RAS-RH [an ultra-filtration extract]) and its underlying mechanisms in human liver cancer cells H22. This study was conducted between September 2010 and August 2012 in the Gansu University of Traditional Chinese Medicine, Lanzhou, China. The groups were assigned as the ...
Sun Zhenchang Z Department of Oncology, the First Affiliated Hospital, Zhengzhou University, - - 2014
The silent Twist gene may increase the sensitivity of cervical cancer cells chemotherapy to paclitaxel, thus was a new idea to improve the efficacy of cancer chemotherapy. The aim was to explore chemotherapy sensitivity of silent Twist gene increased cervical cancer cells to paclitaxel through study the proliferation and apoptosis ...
Tian Ke K Department of Orthopedics, the First Affiliated Hospital, Zhengzhou University,Zhengzhou, - - 2014
MicroRNA has proved to be low expression in many tumor cells. In addition, it was also proved that as a kind of cancer suppressor gene, miR-199a-3p in miRNA can affect the growth and invasion ability of tumor cells. This paper aims to discuss the effect of miRNA to osteosarcoma cell. ...
Zhihua Zhao Z Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, - - 2014
This paper aims to study the effect of telomerase inhibitors zidovudine (AZT) on cell morphology,survival rate and telomerase activity of in-vitro culture myeloma cell line (U266).It has provided experiment basis for applying telomerase inhibitors in multiple myeloma treatment. The myeloma cell line in logarithmic phase (U266) could be divided into ...
Xuekun Huang H Department of Otolaryngology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou city, Guangdong Province, China, - - 2014
Gammadelta-T-cells play an important role in the mucosal immune system of the respiratory tract. Th17 cells plays an important role in promoting inflammation and autoimmune diseases. Th17 cells mainly secrete IL-17. However, little information is available on the relation between gammadelta-T-cells and Th17 cells in allergic rhinitis (AR). To explore ...
Aas Ida Bergva IB Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, 0454 Oslo, - - 2014
Previously, our group has shown that the interbranchial lymphoid tissue (ILT) is a distinct structure largely consisting of T cells embedded in a meshwork of epithelial cells, with no direct resemblance to previously described lymphoid tissues. In this study, we aim to focus on the T cell population and the ...
Xu Jue J Department of Otolaryngology, The First Hospital Affiliated to Huzhou University Medical College, Huzhou, Zhejiang 313000, P.R. - - 2014
Chibby (Cby) inhibits Wnt/β-catenin-mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of β-catenin) to bind to β-catenin. This suggests that Cby could be a tumor suppressor protein. In the present study, we examined Cby expression in laryngeal squamous cell carcinoma (LSCC) and its function and mechanism ...
Song Yan-Qiang - - 2014
BackgroundThe biological processes and molecular mechanisms underlying miR-107 remain unclear in gastric cancer(GC). In this study, we aimed to investigate the expression, biological functions and mechanisms of miR-107 in GC.MethodsQuantitative real-time RT-PCR was used to test miR-107 expression. MTT and colony formation assays were conducted to explore the potential function ...
Kumar Sujeet S Department of Pharmaceutical Chemistry, KLE University's College of Pharmacy, Bangalore 560 010, Karnataka, - - 2014
A series of 2,5,6-substituted imidazo[2,1-b][1,3,4]thiadiazole derivatives have been prepared and were tested for antiproliferative activity on cancer cells at the National Cancer Institute. Results showed that molecules with a benzyl group at position 2, exhibited an increase in activity for the introduction of a formyl group at the 5 position. ...
Kim Tae-Kang TK Departments of Pathology and Laboratory Medicine, Memphis, TN 38163, - - 2014
Melatonin and its metabolites including 6-hydroxymelatonin (6(OH)M), N(1)-acetyl-N(2)-formyl-5-methoxykynuramine (AFMK) and 5-methoxytryptamine (5MT) are endogenously produced in human epidermis. This production depends on race, gender and age. The highest melatonin levels are in young African-Americans [30 to 50 years old (yo)], old Caucasian males (60 to 90 yo) and Caucasian females. ...
Wang W - - 2014
High mobility group box 1(HMGB1) has been reported to be associate with tumor clinical stage and pathological grade in bladder cancer (BC). In this study, we investigated the underlying mechanism through lentivirus-mediated HMGB1 knockdown. HMGB1 was strongly inhibited in BC cells stably transfected with shRNA against HMGB1. MTT and colony ...
Cools S S Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, - - 2014
Fourteen multi- and eight primiparous high-yielding dairy cows were followed from the first till the fourth ovulation postpartum. Cows were randomly divided into two groups and supplemented with soybean (group I; n = 11) or rapeseed meal (group II; n = 11). Both groups were subjected to a biopsy sampling of the corpus luteum ...
Mattfeldt T T Institute of Pathology, University of Ulm, Um, - - 2014
In the present study, 53 cases of squamous cell carcinomas of the head and neck were characterized by a quantitative histological texture analysis based on principles of spatial statistics. A planar tessellation of the epithelial tumour component was generated by a skeletonization algorithm. The size distribution of the virtual cells ...
Wu Changzhu - - 2014
Although several strategies are now available to enzymatically crosslink linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative crosslinking of a hyperbranched polyglycerol (hPG) functionalized with ...
Tang Linlin L Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, - - 2014
Invasion, either directly or via metastasis formation, is the main cause of death in cancer patients. Development of efficient anti-invasive agents is an important research challenge. 6, 6'-bis (2, 3-dimethoxybenzoyl)-a, a-D-trehalose (DMBT), one of brartemicin analogs, was found to be the most potent anti-invasive agent, but the underlying mechanisms are ...
Liu Hong-Chun HC Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: - - 2014
Observe how specific small RNA interference (siRNA) aimed at TPX2 gene suppresses TPX2 gene expression in esophageal cancer EC9706 cells and the effect on esophageal cancer cell growth and invasion ability. Transfect TPX2 siRNA into EC9706 cells via lipofectamin 2000. The experiments were divided into three groups, a negative control, ...
Zeng Zhao-Jun ZJ Molecular Biology Research Center, School of Life Sciences, Central South University, 110, Xiangya Road, Changsha, Hunan 410078, PR China; State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, PR - - 2014
Ganciclovir (GCV) affects the molecular mechanism of cell death and DNA damage by the rAAV (recombinant adeno-associated virus)-mediated Tet-On/HSV-tk/GCV suicide gene system in human breast cancer cell line MCF-7. A rAAV/TRE/Tet-On/HSV-tk combining a Tet-On regulating system and a suicide gene HSV-tk was used to transfect human breast cancer cell line ...
Brossard Dominique D Université de Caen/Basse-Normandie, Esplanade de la Paix, 14032 Caen Cedex, France; UFR des Sciences Pharmaceutiques, Centre d'Etudes et de Recherche sur le Médicament de Normandie (CERMN), CNRS INC3M - SFR ICORE 146, Bd Becquerel, F-14032 Caen Cedex, - - 2014
We previously reported that the cinnamylpiperazinyl group in the side chain of the chenodeoxycholic acid showed apoptosis-inducing activity on multiple myeloma cancer cell line KMS-11. In the present study, we synthesized and tested the pro-apoptotic potency of fifteen new piperazinyl bile carboxamide derived from cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic and lithocholic ...
Chen Daixing D Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, - - 2014
Intestinal barrier damage is an important event during the occurrence and progression of severe acute pancreatitis. The expression of occludin, one of the main components of the intestinal barrier proteins, is regulated by various factors related to intestinal barrier formation and the remodeling process. The αSNAP, as a novel membrane ...
Toossi Mohammad Taghi Bahreyni MT Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran ; Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, - - 2014
Radiation damages initiated by radiation-induced bystander effect (RIBE) are not limited to the first or immediate neighbors of the irradiated cells, but the effects have been observed in the cells far from the irradiation site. It has been postulated that bystander cells, by producing bystander factors, are actively involved in ...
Lombardi Leonardo Augusto LA Morphology and Genetics Department, Paulista Medical School, Federal University of São Paulo, São Paulo, SP, - - 2014
to evaluate the immunohistochemical expression of proliferative, apoptotic and steroidogenic enzyme markers in the ovaries of rats with polycystic ovary syndrome (PCOS). twenty rats were divided into two groups: GCtrl - estrous phase, and PCOS - with polycystic ovaries. The GCtrl animals were subjected to a lighting period from 7 ...
Song Jianghong J Department of Ultrasound Diagnosis, Affiliated Hospital of Yan'an University, Yan'an, Shaanxi Province, P.R. - - 2014
Purpose: To explore the regulatory roles of antisense oligonucleotide (ASODN) of vascular endothelial growth factor receptor 2/ VEGFR2 (kinase insert domain-containing receptor, KDR) on the proliferation of PC-3 human prostate cancer cell line. Methods: Different concentrations of synthetic sense and antisense KDR oligonucleotides were transfected into PC-3 human prostate cancer ...
Liu Min Li ML Department of Pathology, Yan'an University School of Medicine, Yan'an 716000, Shaannxi Province, - - 2014
Purpose: This study aimed to investigate the effects of resveratrol on cell proliferation, apoptosis and protein expression of survivin in human gastric cancer (SGC7901) cell line. Methods: SGC7901 cell proliferation and G0/G1 phase of the cell cycle induced by resveratrol (treatment group) and phosphate buffer solution (PBS) (control group) were ...
González-Morán María Genoveva MG Facultad de Ciencias, Laboratorio de Biología de la Reproducción Animal, Departamento de Biología Comparada, Universidad Nacional Autónoma de México. Mexico, D.F. 04510, Mexico. Electronic address: - - 2014
In this work, the presence of estrogen receptor alpha (ER-α) was determined in different cell subpopulations in the left growing and right regressing ovaries of Gallus domesticus from 13-day-old chicken embryos to one-month-old chickens by immunohistochemistry. Results revealed positive ER-α immunostainig in both ovaries during development, but the percentage, staining ...
Zou Ting T Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, - - 2014
In this study, we established cell models for Parkinson's disease using rotenone. An RNA interference vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with ...
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