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Results 1 - 50 of 2018
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Sima Jin J Department of Urology, Aerospace Central Hospital, No. 15 Yuquan Road, Beijing, 100049, - - 2014
The objective of the study was to investigate the impact of Numb on cell growth, cell migration, and invasion in human clear cell renal cell carcinoma (ccRCC). Endogenous expression of Numb was evaluated in the ccRCC cell lines (786-O, Caki-1, and Caki-2) and control reference human renal proximal tubular epithelial ...
Dhyani Anamika A Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, Brazil. Electronic address: - - 2014
ANKHD1 (Ankyrin repeat and KH domain-containing protein 1) is highly expressed and plays an important role in the proliferation and cell cycle progression of multiple myeloma (MM) cells. ANKHD1 downregulation modulates cell cycle gene expression and upregulates p21 irrespective of the TP53 mutational status of MM cell lines. The present ...
Ahn Ji Hyeon JH Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South - - 2014
Hippophae rhamnoides L. (HL) exerts antioxidant activities against various oxidative stress conditions. In this study, we investigated effects of extract from HL leaves (HLE) on cell proliferation and neuroblast differentiation in the subgranular zone (SGZ) of the dentate gyrus (DG) of aged gerbils. Aged gerbils (24 months) were divided into ...
Wang Bo B 1 College of Veterinary Medicine, Northwest A&F University , Yangling, 712100, Shaanxi, China - - 2014
Abstract The essence of the reprogramming activity of somatic cell nuclear transfer (SCNT) embryos is to produce normal fertilized embryos. However, reprogramming of somatic cells is not as efficient as the reprogramming of sperm. In this report, we describe the effect of an inducible, specific miR-34 microRNA expression in donor ...
Choi Y B YB Seoul National University, Seoul, South - - 2014
Donggyeong dog is a breed considered a natural monument in Korea since 2012. Nevertheless, this breed is only found in Gyeong-ju and is classified as endangered. The use of assisted reproductive technologies (ART) could be applied to preserve these endangered dogs. Among various ART, somatic cell nuclear transfer (SCNT) may ...
Martins L T LT University of Fortaleza (UNIFOR), Fortaleza, CE, - - 2014
The generation of transgenic cell lines through standard cell transfection/antibiotic selection procedures may have a negative effect on cell viability, which in turn may compromise SCNT cloning efficiency. The aim of this study was to evaluate goat cloning efficiency by using transfected and nontransfected and transgenic and nontransgenic somatic cells ...
Sepulveda-Rincon L P LP Child Health, Obstetrics and Gynaecology, University of Nottingham, Nottingham, United - - 2014
The first lineage specification occurs during pre-implantation mammalian development. At the blastocyst stage, 2 cell lineages can be distinguished: the inner cell mass (ICM) and the trophectoderm (TE). The exact timing when embryo cells are skewed to these lineages is not clearly determined in mammalian species. In murine embryos, it ...
Song K K Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, - - 2014
Immortalization of somatic cells by oncogenes is effective for evaluation of gene targeting tools or molecular cell pathway because of relatively longer life span than normal cells. The BMI1 gene has been used to immortalize human and murine somatic cells, and we confirmed that BMI1 increased cell-life span of porcine ...
Dores C C Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, - - 2014
In vertebrates, the primary cilium is a nearly ubiquitous organelle present in somatic cells, but little is known about its function in the male gonad. We investigated the role of primary cilia in testis cells using in vitro formation of seminiferous tubules and in vitro culture of testicular somatic cells ...
Cai L L VETEMBIO, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of - - 2014
Granulocyte colony-stimulating factor (G-CSF), also known as colony-stimulating factor 3 (CSF3), is required for the proliferation, differentiation, and survival of cells. In humans, G-CSF is a biomarker of human oocyte developmental competence for embryo implantation. Furthermore, G-CSF concentration increases during the menstrual cycle and levels were significantly higher during ovulatory ...
Wang Xinjie X Integrated TCM and Western Medicine Department, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China, - - 2014
The aim of this study was to study RAS-siRNA blocking RAS pathway and suppressing cell growth in human oesophageal squamous cell carcinoma in nude mice. The methods in this study was to construct RAS-siRNA expression vector, establish 40 oesophageal squamous cell carcinoma xenograft animal models and divided them into five ...
Zhang Xuefeng X Department of Pathology, Duke University Medical Center, Durham, NC, - - 2014
The diagnosis of Barrett's esophagus in the United States requires both endoscopically evident columnar-lined esophagus and the presence of goblet cells by histology. Currently, there is no consensus on how patients with nongoblet columnar-lined esophagus should be followed. In this study, we investigated whether biomarkers can be used to predict ...
Gu Yanling Y College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of - - 2014
Bioremediation of hexavalent chromium by Aspergillus niger was attributed to the reduction product (trivalent chromium) that could be removed in precipitation and immobilized inside the fungal cells and on the surface of mycelium. The site location of reduction was conducted with assays of the permeabilized cells, cell-free extracts, and cell ...
Sánchez-Quesada Cristina C Immunology Division, Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Campus las Lagunillas s/n, 23071 Jaén, Spain. jgaforio@ujaen.es csquesad@ujaen.es - - 2014
Uvaol (UV) and erythrodiol (ER) are two triterpenic dialcohols present in the minor fraction of virgin olive oil, in leaves and in the drupe of olives. These triterpenes possess the same chemical structure and differ only in the location of a methyl group. It has been reported that they have ...
Goodwin Daryn D School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, 4072, Queensland, - - 2014
In this study we aimed to address the poor drug-like properties of Gonadotropin-Releasing Hormone (GnRH) peptide through modification with lipids and carbohydrates. GnRH peptide was conjugated to 2-amino-D,L-octanoic acid (C8) and 2-amino-D,L-dodecanoic acid (C12) in monomer and dimer, along with (6-9) or without (2-5 and 11) a glucose moiety. Peptides ...
Liu Wen-Xin WX Department of Gynecologic Neoplasms, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. - - 2014
The aim of the present study was to determine the effect of cyclin‑dependent kinase 7 (CDK7) silencing on the sensitivity of the HEC‑1‑A endometrial carcinoma cell line to cisplatin [cis‑dichlorodiammineplatinum (II), or DDP]. Four CDK7 siRNA fragments were designed and synthesized based on the gene sequence of CDK7 and transfected into ...
Torbidoni Ana Vanesa AV Department of Hemato-Oncology, Pediatric Hospital "Prof. Dr. Juan P. Garrahan", Buenos Aires, - - 2014
The identification of molecules expressed selectively on the surface of retinoblastoma cells would allow applying targeted therapies. The Ganglioside, N-Glycolyl-GM3 (NeuGc-GM3), is an attractive candidate, as it has been detected in other paediatric neuroectodermic tumours, and it is not expressed in human normal tissues. The 14F7 antibody recognizes specifically the ...
Wang Jia J Department of Microbiology, Dalian Medical University, Dalian 116044, - - 2014
The shiitake mushroom Lentinula edodes has health benefits and is used to treat various diseases due to its immunomodulatory and antineoplastic properties. In the present study, the Latcripin-13 domain, isolated from L. edodes, was expressed in Escherichia coli Rosetta-gami(DE3) in the form of inclusion bodies. The Latcripin-13 domain was purified ...
Kamao Hiroyuki H Ophthalmology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192, Japan - - 2014
Purpose For the transplantation of human induced pluripotent stem cell-derived retinal pigment epithelium (hiPSC-RPE), determination of the maturation status of these cells is essential, and the degree of pigmentation (dPG) can serve as a good indicator of this status. The aim of this study was to establish a method of ...
Sun Yongming Y Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. - - 2014
Resveratrol has been shown to have antineoplastic effects in vivo and in vitro. However, the effect of resveratrol on the hypoxia-enhanced proliferation and invasion of osteosarcoma cells remains unclear. In this study, we investigated the role of resveratrol on regulating proliferation and invasion of osteosarcoma cells under hypoxic conditions. Saos-2 cells were ...
Magoulas George E GE Laboratory of Synthetic Organic Chemistry, Department of Chemistry, University of Patras, 26504, Patras, - - 2014
To synthesize pegylated stoichiometrically and structurally well-defined conjugates of fullerene (C60) with doxorubicin (DOX) and investigate their antiproliferative effect against cancer cell lines. Stoichiometric (1:1 and 1:2) pegylated conjugates of C60 with DOX were synthesized using the Prato reaction to create fulleropyrrolidines equipped with a carboxyl function for anchoring a ...
Deng Ying-Hu YH Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. - - 2014
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The ...
Kikuta J J Department of Orthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, - - 2014
In this study, we first investigated the expressions of Jagged1, Notch2, the receptor activator of nuclear factor-kappa B ligand (RANKL), and interleukin (IL)-6 in areas of root resorption during experimental tooth movement in rats in vivo. We then assessed the effects of compression force (CF) with or without GSI (an inhibitor ...
Goldman Elisheva Brina EB Hebrew University, Institute of Dental Sciences, Jerusalem, Israel ; - - 2014
Impaired salivary glands' (SG) function leading to oral diseases is relatively common with no adequate solution . Previously, tissue engineering of SG had been proposed to overcome this morbidity, however not yet clinically available. Multiwall inorganic (WS2) nanotubes (INT-WS2) and fullerene-like nanoparticles (IF-WS2) have many potential medical applications. A yet ...
Deng Huiling H Department of Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. - - 2014
Hypoxia is a primary mediator for cell survival, and has been reported to inhibit cardiomyocyte proliferation in fetal and neonatal hearts. CyclinA2 is a key regulator of cell proliferation. Whether CyclinA2 affects cardiomyocyte proliferation in hypoxic conditions remains unexamined. This study was designed to investigate the roles of CyclinA2 expression ...
Shen Jian J Department of Cardiothoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. - - 2014
The aim of the present study was to investigate cobra neurotoxin (cobrotoxin) activity in A549 cell lines transplanted into nude mice, and to explore its molecular mechanism. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the growth inhibition rate of cobrotoxin in human lung A549 adenocarcinoma cells and HFL1 ...
Joy Beena B Agroprocessing and Natural Product Division, National Institute for Interdisciplinary Sciences and Technology (CSIR), Thiruvananthapuram, Kerala, 695019, India, - - 2014
Photodynamic therapy (PDT) involves the use of photochemical reactions mediated through the interaction of photosensitizing agent for the treatment of malignant tumor. The present study was carried out to evaluate the photosensitizing potential of embelin isolated from Embelia ribes. For PDT study, cells were grouped into four groups. Two types ...
Kim Myong Ki MK Department of Natural Medicine Resources, Semyung University , Chungbuk , South Korea - - 2014
Abstract This study investigated the neuroprotective effects of Hericium erinaceus mycelium enriched with garlic extract (HGE) on rat pheochromocytoma nerve cells (PC12). The survival rates of the PC12 nerve cells and the neurite-bearing cells after the addition of HGE were estimated as 3.5 × 10(3) viable cells/ml and 2.3 × 10(3) viable cells/ml, respectively, ...
Solarska-Ściuk Katarzyna K Department of Molecular Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: - - 2014
The development of nanotechnology opens up new ways for biomedical applications of unmodified and modified diamond nanoparticles which are one of the most popular nanomaterials used in biology, biotechnology, medicine, cosmetics and engineering. They have been applied as diagnostic and therapeutic agents because they can be targeted to and localized ...
Yang Zuishu Z School of Food Science and Pharmacy, Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, P.R. - - 2014
Ruditapes philippinarum is a member of the Veneridae family of marine bivalve molluscs. RPOI‑1 (Ruditapes philippinarum oligopeptide) is a tetrapeptide that can be extracted from Ruditapes philippinarum by means of enzymolysis. This study showed that RPOI‑1 strongly inhibits proliferation and induces apoptosis in DU‑145 human prostate cancer cells. When cells were treated with ...
Yan Xiangzhen X RadboudUMC, Dentistry, Biomaterials, Nijmegen, Netherlands ; - - 2014
This study aimed to evaluate the in vivo biocompatibility and periodontal regenerative potential of enzymatically solidified chitosan hydrogels with or without incorporated periodontal ligament cells (PDLCs). To this end, chitosan hydrogels, with (n=8; CHIT+CELL) or without (n=8; CHIT) fluorescently-labeled PDLCs, were prepared and transplanted into rat intrabony periodontal defects; untreated ...
Shao Yuan Y Department of Otorhinolaryngology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. - - 2014
The present study aimed to investigate the effect of A disintegrin and metalloproteinase 10 (ADAM10) gene silencing on the proliferation, migration and invasion of the human tongue squamous cell carcinoma cell line TCA8113. RNA interference was used to knock down the expression of ADAM10 in the TCA8113 cell line and ...
Sheng Guojun G Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe 650-0047, Hyogo, Japan. Electronic address: - - 2014
The epiblast is a single cell-layered epithelium which generates through gastrulation all tissues in an amniote embryo proper. Specification of the epiblast as a cell lineage in early development is coupled with that of the trophoblast and hypoblast, two lineages dedicated to forming extramebryonic tissues. The complex relationship between molecular ...
Tourchi Ali A Division of Pediatric Urology, Brady Urological Institute, The Johns Hopkins Medical Institutions, Baltimore, - - 2014
To investigate the association between impaired autophagy in smooth muscle cells and the development of congenital ureteropelvic junction (UPJ) obstruction (UPJO). Tissue specimens were obtained from 40 patients with unilateral UPJO and were divided into 3 sections as renal pelvis, site of obstruction, and the ureter distal to obstruction. Control ...
Braunger Barbara M BM Institute of Human Anatomy and Embryology, University of Regensburg, Universitaetsstr. 31, Regensburg, D-93053, - - 2014
Purpose: To identify stem cells in the chamber angle of the monkey eye by detection of 5-bromo-2'-deoxyuridine (BrdU) long-term retention. Methods: Four cynomolgus monkeys were treated 4 weeks with BrdU via subcutaneous pumps. The eyes of two animals were processed immediately thereafter (group 1) while in the other animals BrdU ...
Marudhupandi Thangapandi T Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences Annamalai University, Parangipettai 608502, Tamil Nadu, India; Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Chennai 600119, India. Electronic address: - - 2014
The present study was conducted to evaluate the anticancer activity of fucoidan isolated from brown seaweed Turbinaria conoides. Extracted fucoidan contained 53±0.69% of fucose and 38±0.42% of sulphate, respectively. Functional groups and structural characteristics of the fucoidan were analyzed by FT-IR and NMR. In vitro anticancer effect was studied on ...
Yan Yulan Y Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. - - 2014
Newcastle disease virus (NDV) is a member of the genus Avulavirus in the Paramyxoviridae family and its antitumor properties depend on its ability to kill malignant cells while not affecting normal cells. The present study investigated a recombinant avirulent NDV LaSota strain (wild‑type NDV strain) expressing the rabies virus glycoprotein (rL‑RVG), examined ...
Li Xuezhen X Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. - - 2014
Notch signaling has been reported to be oncogenic or tumor suppressive, depending on the tissue context. To investigate the effects of Notch2 knockdown on U87 human glioma cell proliferation in vitro and in vivo, and the associated mechanisms, U87 cells were stably transfected with p green fluorescent protein (GFP)‑V‑RS Notch2 short ...
Yamasaki Shinya S Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, Osaka, - - 2014
The purpose of this study was to assess how peripheral blood cells (PBCs) contribute to meniscus repair, using a parabiotic rat model. Wild-type (WT) and green fluorescent protein (GFP) transgenic rats were conjoined at the torso. After 4 weeks, the anterior part of the medial meniscus of both groups of ...
Fick J M JM Department of Applied Physics, University of Eastern Finland, Kuopio FI-70211, Finland. Electronic address: - - 2014
To determine if increasing cartilage cross-links through in vitro glycation of cartilage explants can alter the biomechanical response of chondrocytes to compressive deformation. Bovine osteochondral explants were either incubated with cell culture solution supplemented with (n = 7) or without (n = 7) ribose for 42 h in order to induce glycation. Deformation-induced changes in cell volume, ...
Belal Mahmoud Helmy MH 1 Department of Oral Medicine, Periodontology & Oral Diagnosis, Faculty of Oral & Dental Medicine, Fayoum University , Bahaariy Region, Al-Fayoum, Egypt - - 2014
Abstract Objective: Clinical application of lasers in periodontal therapy has continued to expand in last decades; however there are still some controversies. The present study aimed to compare the conditioning effects of the carbon dioxide (CO2) or erbium-doped: yttrium, aluminum and garnet (Er:YAG) laser on periodontally diseased root surfaces following ...
Ebenezar A V Rajesh AV Professor, Department of Conservative Dentistry & Endodontics, Madha Dental College , Chennai, India - - 2014
Aim: To investigate the viability of PDL cells in an avulsed tooth using a storage medium with and without L-dopa. Materials and Methods: Twenty freshly extracted non-impacted human teeth with closed apices and no caries beyond the CEJ were obtained for this study. The teeth were then randomly divided into ...
Sakagami Hiroshi H Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University, School of Dentistry, - - 2014
The present article reviewed the biological activities and possible dental application of three major polyphenols, i.e., lignin-carbohydrate complexes, tannins, and flavonoids, citing mostly our in vitro studies together with those from other groups. All these polyphenols showed much lower tumor-selective cytotoxicity against oral squamous cell carcinoma cells vs. normal oral ...
Peng Cheng C Department of Urology, 456 Hospital of PLA, No. 25 Wuyingshan Road, Jinan, 250031, - - 2014
Multiple studies have shown that CC motif chemokine ligand 19 (CCL19) promotes cell proliferation in several human cancers. In this study, we investigated the clinical significance of CCL19 and its specific receptor CCR7 and its function in our large collection of prostate samples. Between August 2000 and December 2013, 108 ...
Chen Lei L 1] State Key Laboratory of Palaeobiology and Stratigraphy, and Key Laboratory of Economic Stratigraphy and Palaeogeography, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, China [2] College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, - - 2014
Phosphorites of the Ediacaran Doushantuo Formation (∼600 million years old) yield spheroidal microfossils with a palintomic cell cleavage pattern. These fossils have been variously interpreted as sulphur-oxidizing bacteria, unicellular protists, mesomycetozoean-like holozoans, green algae akin to Volvox, and blastula embryos of early metazoans or bilaterian animals. However, their complete life cycle ...
Rosseti Isabela Bueno - - 2014
Candida species are the fourth most common cause of nosocomial bloodstream infections. It has been observed an increase in the frequency of infections which have become refractory to standard antifungal therapy. Recently, it was demonstrated the effect of different organochalcogenide compounds reducing both growth and germ tube formation by Candida ...
Hussein Shaymaa Subhi SS Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, - - 2014
Mosaicism is present in more than 50% of the cases with small supernumerary marker chromosomes (sSMCs) and karyotype 47,XX,+mar/46,XX or 47,XY,+mar/46,XY. Recently we provided first evidence that the mitotic stability of sSMC is dependent on their structure, i.e. their shape. Thus, here we performed a long term in vitro study ...
Navarini Natalia Festugatto NF Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua José Rocha Junqueira, 13, 13045-610, Campinas, São Paulo, Brazil, - - 2014
During tumor development, benign neoplastic cells are influenced by the expression of cytokines, growth factors, and proteases present in the tumor microenvironment. Epidermal growth factor (EGF) is the most studied growth factor and is considered important for cell proliferation and migration. Metalloproteinases (MMPs) are also involved in tumor progression. The ...
Chen Li L Department of Toxicology, School of Public Health, Wuhan University, Wuhan, Hubei 430071, P.R. - - 2014
This study was designed to investigate the direct effects of fatty acids (FAs) on the cell viability and the expression levels of genes involved in lipid metabolism in LO2 human liver cells. Palmitate (PA), oleate (OA) and docosahaexenoic acid (DHA) were used to represent saturated, mono-unsaturated and polyunsaturated FAs, respectively. ...
Centola Matteo M University Hospital Basel, Departments of Surgery and of Biomedicine, Basel, Switzerland ; - - 2014
Enzymatic isolation of chondrocytes from a cartilage biopsy is the first step to establish in vitro models of chondrogenesis or to generate cell-based grafts for cartilage repair. Such process is based on manually operated procedures and typically results in yields lower than 20% of the total available cells. In this ...
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