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Results 51 - 100 of 1998
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Xu Jue J Department of Otolaryngology, The First Hospital Affiliated to Huzhou University Medical College, Huzhou, Zhejiang 313000, P.R. - - 2014
Chibby (Cby) inhibits Wnt/β-catenin-mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of β-catenin) to bind to β-catenin. This suggests that Cby could be a tumor suppressor protein. In the present study, we examined Cby expression in laryngeal squamous cell carcinoma (LSCC) and its function and mechanism ...
Song Yan-Qiang - - 2014
BackgroundThe biological processes and molecular mechanisms underlying miR-107 remain unclear in gastric cancer(GC). In this study, we aimed to investigate the expression, biological functions and mechanisms of miR-107 in GC.MethodsQuantitative real-time RT-PCR was used to test miR-107 expression. MTT and colony formation assays were conducted to explore the potential function ...
Kumar Sujeet S Department of Pharmaceutical Chemistry, KLE University's College of Pharmacy, Bangalore 560 010, Karnataka, - - 2014
A series of 2,5,6-substituted imidazo[2,1-b][1,3,4]thiadiazole derivatives have been prepared and were tested for antiproliferative activity on cancer cells at the National Cancer Institute. Results showed that molecules with a benzyl group at position 2, exhibited an increase in activity for the introduction of a formyl group at the 5 position. ...
Kim Tae-Kang TK Departments of Pathology and Laboratory Medicine, Memphis, TN 38163, - - 2014
Melatonin and its metabolites including 6-hydroxymelatonin (6(OH)M), N(1)-acetyl-N(2)-formyl-5-methoxykynuramine (AFMK) and 5-methoxytryptamine (5MT) are endogenously produced in human epidermis. This production depends on race, gender and age. The highest melatonin levels are in young African-Americans [30 to 50 years old (yo)], old Caucasian males (60 to 90 yo) and Caucasian females. ...
Wang W - - 2014
High mobility group box 1(HMGB1) has been reported to be associate with tumor clinical stage and pathological grade in bladder cancer (BC). In this study, we investigated the underlying mechanism through lentivirus-mediated HMGB1 knockdown. HMGB1 was strongly inhibited in BC cells stably transfected with shRNA against HMGB1. MTT and colony ...
Cools S S Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, - - 2014
Fourteen multi- and eight primiparous high-yielding dairy cows were followed from the first till the fourth ovulation postpartum. Cows were randomly divided into two groups and supplemented with soybean (group I; n = 11) or rapeseed meal (group II; n = 11). Both groups were subjected to a biopsy sampling of the corpus luteum ...
Mattfeldt T T Institute of Pathology, University of Ulm, Um, - - 2014
In the present study, 53 cases of squamous cell carcinomas of the head and neck were characterized by a quantitative histological texture analysis based on principles of spatial statistics. A planar tessellation of the epithelial tumour component was generated by a skeletonization algorithm. The size distribution of the virtual cells ...
Wu Changzhu - - 2014
Although several strategies are now available to enzymatically crosslink linear polymers to hydrogels for biomedical use, little progress has been reported on the use of dendritic polymers for the same purpose. Herein, we demonstrate that horseradish peroxidase (HRP) successfully catalyzes the oxidative crosslinking of a hyperbranched polyglycerol (hPG) functionalized with ...
Tang Linlin L Department of Pharmacology, School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, - - 2014
Invasion, either directly or via metastasis formation, is the main cause of death in cancer patients. Development of efficient anti-invasive agents is an important research challenge. 6, 6'-bis (2, 3-dimethoxybenzoyl)-a, a-D-trehalose (DMBT), one of brartemicin analogs, was found to be the most potent anti-invasive agent, but the underlying mechanisms are ...
Liu Hong-Chun HC Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: - - 2014
Observe how specific small RNA interference (siRNA) aimed at TPX2 gene suppresses TPX2 gene expression in esophageal cancer EC9706 cells and the effect on esophageal cancer cell growth and invasion ability. Transfect TPX2 siRNA into EC9706 cells via lipofectamin 2000. The experiments were divided into three groups, a negative control, ...
Zeng Zhao-Jun ZJ Molecular Biology Research Center, School of Life Sciences, Central South University, 110, Xiangya Road, Changsha, Hunan 410078, PR China; State Key Laboratory of Medical Genetics, Central South University, Changsha 410078, PR - - 2014
Ganciclovir (GCV) affects the molecular mechanism of cell death and DNA damage by the rAAV (recombinant adeno-associated virus)-mediated Tet-On/HSV-tk/GCV suicide gene system in human breast cancer cell line MCF-7. A rAAV/TRE/Tet-On/HSV-tk combining a Tet-On regulating system and a suicide gene HSV-tk was used to transfect human breast cancer cell line ...
Brossard Dominique D Université de Caen/Basse-Normandie, Esplanade de la Paix, 14032 Caen Cedex, France; UFR des Sciences Pharmaceutiques, Centre d'Etudes et de Recherche sur le Médicament de Normandie (CERMN), CNRS INC3M - SFR ICORE 146, Bd Becquerel, F-14032 Caen Cedex, - - 2014
We previously reported that the cinnamylpiperazinyl group in the side chain of the chenodeoxycholic acid showed apoptosis-inducing activity on multiple myeloma cancer cell line KMS-11. In the present study, we synthesized and tested the pro-apoptotic potency of fifteen new piperazinyl bile carboxamide derived from cholic, ursodeoxycholic, chenodeoxycholic, deoxycholic and lithocholic ...
Chen Daixing D Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, - - 2014
Intestinal barrier damage is an important event during the occurrence and progression of severe acute pancreatitis. The expression of occludin, one of the main components of the intestinal barrier proteins, is regulated by various factors related to intestinal barrier formation and the remodeling process. The αSNAP, as a novel membrane ...
Toossi Mohammad Taghi Bahreyni MT Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran ; Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, - - 2014
Radiation damages initiated by radiation-induced bystander effect (RIBE) are not limited to the first or immediate neighbors of the irradiated cells, but the effects have been observed in the cells far from the irradiation site. It has been postulated that bystander cells, by producing bystander factors, are actively involved in ...
Lombardi Leonardo Augusto LA Morphology and Genetics Department, Paulista Medical School, Federal University of São Paulo, São Paulo, SP, - - 2014
to evaluate the immunohistochemical expression of proliferative, apoptotic and steroidogenic enzyme markers in the ovaries of rats with polycystic ovary syndrome (PCOS). twenty rats were divided into two groups: GCtrl - estrous phase, and PCOS - with polycystic ovaries. The GCtrl animals were subjected to a lighting period from 7 ...
Song Jianghong J Department of Ultrasound Diagnosis, Affiliated Hospital of Yan'an University, Yan'an, Shaanxi Province, P.R. - - 2014
Purpose: To explore the regulatory roles of antisense oligonucleotide (ASODN) of vascular endothelial growth factor receptor 2/ VEGFR2 (kinase insert domain-containing receptor, KDR) on the proliferation of PC-3 human prostate cancer cell line. Methods: Different concentrations of synthetic sense and antisense KDR oligonucleotides were transfected into PC-3 human prostate cancer ...
Liu Min Li ML Department of Pathology, Yan'an University School of Medicine, Yan'an 716000, Shaannxi Province, - - 2014
Purpose: This study aimed to investigate the effects of resveratrol on cell proliferation, apoptosis and protein expression of survivin in human gastric cancer (SGC7901) cell line. Methods: SGC7901 cell proliferation and G0/G1 phase of the cell cycle induced by resveratrol (treatment group) and phosphate buffer solution (PBS) (control group) were ...
González-Morán María Genoveva MG Facultad de Ciencias, Laboratorio de Biología de la Reproducción Animal, Departamento de Biología Comparada, Universidad Nacional Autónoma de México. Mexico, D.F. 04510, Mexico. Electronic address: - - 2014
In this work, the presence of estrogen receptor alpha (ER-α) was determined in different cell subpopulations in the left growing and right regressing ovaries of Gallus domesticus from 13-day-old chicken embryos to one-month-old chickens by immunohistochemistry. Results revealed positive ER-α immunostainig in both ovaries during development, but the percentage, staining ...
Zou Ting T Department of Neurology, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, - - 2014
In this study, we established cell models for Parkinson's disease using rotenone. An RNA interference vector targeting Parkin-associated endothelin receptor-like receptor (Pael-R) was transfected into the model cells. The results of reverse-transcription polymerase chain reaction and western blot analysis showed that Pael-R expression was decreased after RNA interference compared with ...
Hen Gideon G Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food & Environment, The Hebrew University of Jerusalem, Rehovot, Israel; ARO, Volcani Center, Bet-Dagan, - - 2014
Despite the importance of the chicken as a model system, our understanding of the development of chicken primordial germ cells (PGCs) is far from complete. Here we characterized the morphology of PGCs at different developmental stages, their migration pattern in the dorsal mesentery of the chicken embryo, and the distribution ...
Jordan Brian J BJ Department of Poultry Science, The University of Georgia, 110 Cedar Street, Athens, Georgia 30602, USA; Poultry Diagnostic and Research Center, The University of Georgia, 953 College Station Rd., Athens, Georgia, 30602, USA. Electronic address: - - 2014
The chicken is a well-established model system for studying developmental biology and is recognized as one of the top food production animals in the world. For this reason the chicken is an excellent candidate for transgenic applications, as the technology can be applied to both areas of research. Transgenic technology ...
Zhou Yue Y School of Pharmacy, 2Department of Breast Surgery , The Second Clinical Hospital, 3Department of Plastic Surgery, the China- Japan Union Hospital, 4Department of Biology and Medical Engineering, Institute of Regenerative Medicine, Jilin University, Changchun, China E-mail : gbf_cc@sohu.com, - - 2014
The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells. MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by ...
Zhang Ying Y Department of Immunology, Shandong University School of Medicine, Jinan 250012, - - 2014
Resistance to anoikis and Epithelial-mesenchymal transition (EMT) are two processes critically involved in cancer metastasis. In this study, we demonstrated that after anchorage deprival, hepatocellular carcinoma (HCC) cells not only resisted anoikis, but also exhibited EMT process. Microarray expression profiling revealed that expression of miR-424-5p was significantly decreased in anoikis-resistant ...
Ferron Pierre-Jean PJ ANSES, Fougères Laboratory, Toxicology of Contaminants Unit, French Agency for Food, Environmental and Occupational Health & Safety, Fougères 35302, - - 2014
The phycotoxin, okadaic acid (OA) and dinophysistoxin 1 and 2 (DTX-1 and -2) are protein phosphatase PP2A and PP1 inhibitors involved in diarrhetic shellfish poisoning (DSP). Data on the toxicity of the OA-group toxins show some differences with respect to the in vivo acute toxicity between the toxin members. In ...
Wang H J HJ Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, - - 2014
This study aimed to discuss the effect and mechanism of glutathione (GSH) and catalase (CAT) on transforming growth factor beta (TGF-β)-induced lens epithelial cell (LEC) apoptosis and epithelial mesenchymal transition (EMT) to prevent and control cataracts. Healthy rabbits (4 weeks old) were randomly selected, and LECs from their lenses were ...
Cai Yong Y Department of Radiation Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China E-mail : - - 2014
To explore the radiosensitization effect of overexpression of silent information regulator 6 (SIRT6) on A549 non-small cell lung cancer (NSCLC) cells. Adenovirus vector Ad-SIRT6 causing overexpression of SIRT6 was established. Western blotting and MTT assay were adopted to detect the level of SIRT6 protein and the inhibitory rate of A549 ...
Yang Rui R School of Medicine, Southeast University, Nanjing 210009, - - 2014
20(s)-ginsenoside Rg3 is extracted from traditional Chinese medicine, red ginseng. However, due to its poor aqueous solubility and low oral bioavailability, the use of 20(s)-Rg3 is limited. This study aimed to explore a method of preparing nano-sized 20(s)-ginsenoside Rg3 particle named 20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres (20(s)-Rg3/HSAMNP) to ...
Zhou Yuan Y Department of Oncology, Affiliated Hospital of Guangdong Medical College Zhanjiang 524001, - - 2014
To establish a human hepatoma HepG2 cell line with stable expression of Prolyl hydroxylase domain 3 (PHD3) gene and study its effect of growth and proliferation in nude mice xenograft tumor. Eukaryotic expression vectors of pcDNA 3.1-PHD3 was constructed. HepG2 cells were transfected with recombinant plasmid pcDNA 3.1-PHD3 and empty ...
Shokrzadeh Mohammad M Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran ; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, - - 2014
Edaravone, an antioxidant and radical scavenger, showed protective effects against oxidative stress-like condition. Paraquat (PQ) is toxic herbicide considerable evidence suggests that oxidative stress and mitochondrial dysfunction contribute to PQ toxicity. In this study, protective effect of edaravone against PQ induced toxicity and reactive oxygen species (ROS) generation in A549 ...
Nakhjavani Maryam M Department of Pharmaco-Toxicology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. P.O.Box - - 2014
Cancer is considered as a challenging deathly disease and discovering or synthesis of new cytotoxic agents is a worldwide attempt. In this study, a group of recently synthesized chalcones, with the structure of 1,3-diarylprop-2-en-1-one having different COX-1 and/or COX-2 selectivities have been examined on human hepatocarcinoma (HepG2), lung carcinoma (A549), ...
Shi Qing-Qiang QQ Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China E-mail : lijingyangyang@126.com.cn; - - 2014
To investigate the effect of deacetylase inhibitory trichostatin A (TSA) on anti HepG2 liver carcinoma cells and explore the underlying mechanisms. HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72h were examined for cell growth inhibition using CCK8, changes in cell cycle distribution with flow cytometry, ...
Hou Shaozhang S Department of Pathology, Ningxia Medical University, Yinchuan 750004, China. - - 2014
The aim of this study was to determine the beneficial effect of glycyrrhizic acid (GA) on type 2 diabetic nephropathy using renal tubular epithelial cell line (NRK-52E). The cells are divided into normal group (NG), high glucose group (HG), and treatment group (HG + GA). The methylthiazoletetrazolium (MTT) assay was ...
ElGhamrawy T A TA 1Department of Anatomy, Faculty of Medicine, Cairo University, Cario, Egypt. - - 2014
Sertoli cells are important in determining the fate of spermatogenic cells by providing nutrition and structural support via cell junctions. Adhesion between Sertoli and germ cells is important for spermatogenesis. Cadherin are transmembrane proteins that mediate cell-cell adhesion, while, vimentin, the cytoskeletal intermediate filament plays an important role in spermatogenesis. ...
Zou Tang-Bin TB School of Public Health, Guangdong Medical College, Dongguan 523808, China. - - 2014
Anthocyanins have multiple biological activities of benefit to human health. While a few studies have been conducted to evaluate the bioavailability of anthocyanins, the mechanisms of their absorption mechanism remain ill-defined. In the present study, we investigated the absorption mechanism of cyanidin-3-O-β-glucoside (Cy-3-G) in human intestinal epithelial (Caco-2) cells. Cy-3-G ...
Tang Xiaolong X Stem cell Engineering Research Center, School of Medicine, Anhui University of Science and Technology Huainan 232001, P.R. China ; The State Key Laboratory of Virology, Life Sciences College, Wuhan University Wuhan, Hubei 430072, P.R. - - 2014
The efficient delivery of therapeutic molecule agents into target cells of interest is a critical challenge to broad application of non-viral vector systems. In this research, maytansine-loaded star-shaped folate-core polylactide-D-α-tocopheryl polyethylene glycol 1000 succinate (FA-PLA-TPGS) block copolymer was applied to be a vector of maytansine for folate receptor positive (FR(+)) ...
Chew Yik Ling YL Faculty of Pharmaceutical Sciences, UCSI University Kuala, Lumpur. - - 2014
Flowers of Bauhinia kockiana were investigated for their anticancer properties. Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce ...
Wu Wenxin W Department of Osteology, Third Xiangya Hospital, Central South University Changsha 410013, Hunan, - - 2014
This study was conducted to explore the role of autophagy in cisplatin-resistant osteosarcoma. Cisplatin-resistant osteosarcoma cell line (MG63/DDP) was obtained from parental MG63 by treating cisplatin with an intermittent stepwise selection protocol. The autophagy in MG63/DDP and MG63 was fully analyzed by immunofluorescence and western blot analysis. Meanwhile, the autophagy ...
Kim Ji-Young JY Department of Neurology, Inje University Seoul Paik Hospital, Seoul, South - - 2013
Although alpha-synuclein is generally thought to have a pathological role in Parkinson's disease, accumulative evidence exists that alpha-synuclein has a neuroprotective effect. The aim of this study was to evaluate the effect of extracellular alpha-synuclein on dopaminergic cell survival. We assessed cell viability using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltertazolium bromide (MTT) assay both ...
Meyer Wilfried W Institute for Anatomy, University of Veterinary Medicine Hannover Foundation, 30173 Hannover, - - 2013
Based on immunohistochemistry, the study demonstrates the varying distribution and reaction intensity of connexins (Cx26 [chicken 31sim], 30 [chicken 31], 31, 32, 43, 45) in the developing feather follicle of the chicken (White Leghorn). The different embryonal stages were identified according to the normal table of Hamburger and Hamilton (1951). ...
Chen Zhao - - 2013
Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with non-adapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20, 30, 40, and 50% moisture contents for a 24-h ...
Tyack Scott G - - 2013
Traditional methods of avian transgenesis involve complex manipulations involving either retroviral infection of blastoderms or the ex vivo manipulation of primordial germ cells (PGCs) followed by injection of the cells back into a recipient embryo. Unlike in mammalian systems, avian embryonic PGCs undergo a migration through the vasculature on their ...
Yang Xiaomeng X Shandong Medicinal Biotechnology Center, Key Laboratory for Biotech Drugs of the Ministry of Health, Key Laboratory for Rare Diseases of Shandong Province, Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; ; Ji'nan University Shandong Academy of Medical Sciences College of Life Science and Medicine, Ji'nan, Shandong, - - 2013
The purpose of this study was to investigate optimal concentrations of zoledronic acid (ZA) in terms of their effect on the proliferation, differentiation, and mineralization of primary osteoblasts (OBs) and fibroblasts (FBs). Primary OBs and FBs isolated from patients with clinical osteogenesis imperfecta (OI) and developmental dysplasia of the hip ...
Chen Hong - - 2013
The in vitro production of gametocytes and oocysts of the apicomplexan parasite genus Eimeria is still a challenge in coccidiosis research. Until today, an in vitro development of gametocytes or oocysts had only been shown in some Eimeria species. For several mammalian Eimeria species, partial developments could be achieved in ...
Park Tae Sub TS WCU Biomodulation Major, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, - - 2013
Germ cell differentiation in reverse-sexed reproductive organs and interspecies germ line chimeras provides insight into the mechanism of germ cell development and represents a useful tool for conservation of endangered birds. We investigated the migration and survival capacity of male chicken primordial germ cells (PGCs) in female chicken embryos and ...
Meng Xiao-Xiao XX Department of Critical Care Medicine, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, - - 2013
Ulinastatin (UTI) is a urinary trypsin inhibitor extracted and purified from urine of males. This study aimed to explore the effects of UTI on paraquat-induced-oxidative stress in human type II alveolar epithelial cells. The human type II alveolar epithelial cells, A549 cells, were cultured in vitro. The A549 cells were ...
Nishijima Ken-Ichi - - 2012
The development of transgenic chicken technology has lagged far behind that of mammalian species. Two reasons for this are that only a one-cell-stage oocyte can be obtained from a sacrificed hen and that the yolk prevents high-magnification microscopic observation of oocytes. Recently, several new methods have been developed that will ...
Bayat Parvin-Dokht PD Department of Anatomy, Arak University of Medical Sciences, Arak, - - 2012
Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields. The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the number and quality of mice blastocysts. In present study, 66 ...
Cheng Zhi-Qiang ZQ Department of Emergency Medicine, Affiliated Union Hospital of Huazhong University of Science and Technology, Wuhan 430022, - - 2012
Edaravone (3-methyl-1-penyl-2-pyrazolin-5-one) is a potent free-radical scavenger and has the antioxidant ability to inhibit lipid peroxidation. The study aimed to examine the effect of edaravone on protecting the acute injury of human type II alveolar epithelial cells (A549 cells) induced by paraquat (PQ) and the change of production of reactive ...
Cozad Matthew J - - 2011
One million Americans suffer from chronic wounds every year with diabetics and older populations representing the majority. Mechanisms that may be responsible for the reduced healing response in these patients include reduction in growth factors or vascularization and an increase in free radical levels. The focus of this study was ...
Sugaya Kimihiko - - 2011
A temperature-sensitive (ts) CHO-K1 mutant cell line, tsTM18, exhibits chromosomal instability with decreased DNA synthesis at the nonpermissive temperature, 39oC. An amino acid substitution in Smu1 underlying the ts phenotypes of tsTM18 cells was identified previously. We also found a ts defect in splicing of the unc52/perlecan gene. In the ...
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