Search Results
Results 51 - 100 of 1383
1 2 3 4 5 6 7 8 9 10 >
Reja Shahi Imam SI Department of Chemistry, UGC Sponsored Centre for Advanced Studies-1, Guru Nanak Dev University, Amritsar, Punjab, India. - - 2014
An oxime based fluorescent probe has been designed and synthesized, which detects free as well as enzymatically generated hypochlorite with a low detection limit and high sensitivity. In addition, the probe was successfully utilized for the monitoring of endogenously produced hypochlorite in LPS stimulated cell lines, C6 glioma and BV2 ...
Kim Jeehwan J IBM T. J. Watson Research Center, Yorktown Heights, NY, 10598, - - 2014
High efficiency Cu2 ZnSn(S,Se)4 solar cells are reported by applying In2 S3 /CdS double emitters. This new structure offers high doping concentration within the Cu2 ZnSn(S,Se)4 solar cells resulting in a substantial enhancement in open circuit voltage. The 12.4% device is obtained with a record open circuit voltage deficit of ...
Sheu Jinn-Kong - - 2014
InGaN/GaN-based solar cells with vertical-conduction feature on silicon substrates were fabricated by wafer bonding technique. The vertical solar cells with a metal reflector sandwiched between the GaN-based epitaxial layers and the Si substrate could increase the effective thickness of the absorption layer. Given that the thermally resistive sapphire substrates were ...
Marjanovič Igor I Laboratory of Biocybernetics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000, Ljubljana, - - 2014
In this study, we compared three different methods used for quantification of gene electrotransfer efficiency: fluorescence microscopy, flow cytometry and spectrofluorometry. We used CHO and B16 cells in a suspension and plasmid coding for GFP. The aim of this study was to compare and analyse the results obtained by fluorescence ...
Kang Min-Cheol MC Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of - - 2014
We investigated the protective effect of octaphlorethol A (OPA) isolated from Ishige foliacea against high-glucose-induced oxidative damage, as indicated by reactive oxygen species (ROS) generation, lipid peroxidation, and cell death, in human umbilical vein endothelial cells (HUVECs) and in a zebrafish model. OPA treatment significantly and dose-dependently decreased ROS generation, ...
Bansode Atul A Institute of Chemical Research of Catalonia (ICIQ), Av. Països Catalans 16, 43007 Tarragona, - - 2014
We demonstrate the use of commercially available fused silica capillary and fittings to construct a cell for operando X-ray absorption spectroscopy (XAS) for the study of heterogeneously catalyzed reactions under high pressure (up to 200 bars) and high temperature (up to 280 °C) conditions. As the first demonstration, the cell was ...
Shahveisi Kaveh K Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, - - 2014
Hyperglycemia, oxidative stress and apoptosis have key roles in pathogenesis of diabetic neuropathy. There are local renin-angiotensin systems (RASs) in different tissues such as neural tissue. Local RASs are involved in physiological and pathophysiological processes such as inflammation, proliferation and apoptosis. This study aimed to investigate the role of local ...
Li Menghua M Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants, Bioengineering College, Chongqing University, Chongqing, - - 2014
Adverse effects of nickel ions being released into the living organism have resulted in development of high nitrogen nickel-free austenitic stainless steels for medical applications. Nitrogen not only replaces nickel for austenitic structure stability but also improves steel properties. The cell cytocompatibility, blood compatibility and cell response of high nitrogen ...
Qu Ling L Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, China Academy of Medical Sciences, Beijing, - - 2014
Quercetin can reverse high glucose-induced inhibition of neural cell proliferation, and therefore may have a neuroprotective effect in diabetic peripheral neuropathy. It is difficult to obtain primary Schwann cells and RSC96 cells could replace primary Schwann cells in studies of the role of autophagy in the mechanism underlying diabetic peripheral ...
Birajdar Smita Shrishail SS Department of Oral Pathology and Microbiology, Vishnu Dental College and Hospital, Vishnupur, Bhimavaram, Andhra Pradesh, - - 2014
To demonstrate the presence, location and pattern of cell proliferation in different histological grades of oral epithelial dysplasia (OED), oral squamous cell carcinoma (OSCC) and normal oral epithelium (NOE) using an antibody directed against the Ki-67 antigen and its intensity of staining evaluated respectively. A total number of 100 archival ...
Komai Yoshihiro Y 1] Department of Stem Cell Pathology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, Japan [2] Department of Urology and Andrology, Kansai Medical University, 2-5-1 Shin-machi, Osaka 573-1010, - - 2014
Asingle cells in undifferentiated spermatogonia are considered to be the most primitive forms of germ stem cells (GSCs). Although GFRα1 is thought to be a marker of Asingle cells, we found that Bmi1(High) is more specific than GFRα1 for Asingle cells. Bmi1(High) expression in Asingle cells is correlated with seminiferous ...
Narayana Modugumudi Ananta Satya MA Department of Pathology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, - - 2014
Cutaneous metastasis from transitional cell carcinoma urinary bladder is a rare clinical entity associated with poor prognosis. This case report describes a 51-year-old male who presented with multiple cutaneous metastases arising from high grade transitional cell carcinoma bladder with lamina propria invasion. The prominent cytological features include medium sized to ...
Ali Muhammad M INSERM U1026, Bioingénierie Tissulaire (BioTis) Université Bordeaux Segalen 146, rue Léo-Saignat Case 45 33076 BORDEAUX - - 2014
Laser-assisted bioprinting is a versatile, non-contact, nozzle-free printing technique which has demonstrated high potential for cell printing with high resolution. Improving cell viability requires determining printing conditions which minimize shear stress for cells within the jet and cell impact at droplet landing. In this context, this study deals with laser-induced ...
Gryshkov Oleksandr O Institute for Multiphase Processes, Leibniz Universitaet Hannover, Hannover, - - 2014
Alginate cell-based therapy requires further development focused on clinical application. To assess engraftment, risk of mutations and therapeutic benefit studies should be performed in an appropriate non-human primate model, such as the common marmoset (Callithrix jacchus). In this work we encapsulated amnion derived multipotent stromal cells (MSCs) from Callithrix jacchus ...
Liang Yijian Y State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, - - 2014
High-throughput measurement of huntingtin (Htt) levels is useful for Huntington's disease research. For example, identification of genetic or chemical modifiers that reduce Htt levels by high-throughput screening provides promising strategy for HD drug discovery. In the human cells, high-throughput measurement of Htt levels has been established based on the Time ...
Gorden Brandi H BH Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, 1352 Boyd Avenue, Saint Paul, MN 55108, - - 2014
Human angiosarcoma and canine hemangiosarcoma are thought to arise from vascular tissue or vascular forming cells based upon their histological appearance. However, recent evidence indicates a hematopoietic or angioblastic cell of origin for these tumors. In support of this idea, we previously identified an endothelial-myeloid progenitor cell population with high ...
Kelesidis Theodoros T Department of Medicine, University of California Los Angeles, Los Angeles, California, United States of - - 2014
Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p<0.001) ...
Wagner Andrej A Department of Internal Medicine I, Paracelsus Medical University/Salzburger Landeskliniken (SALK), Muellner Hauptstrasse 48, Salzburg 5020, Austria. - - 2014
Photodynamic therapy (PDT) is a palliative treatment option for unresectable hilar biliary tract cancer (BTC) showing a considerable benefit for survival and quality of life with few side effects. Currently, factors determining the cellular response of BTC cells towards PDT are unknown. Due to their multifaceted nature, microRNAs (miRs) are ...
Knudsen Jan Dines JD Division of Applied Microbiology, Department of Chemistry, Lund University, Lund, 221 00, SE, - - 2014
A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase double deletion ...
Alexiou George A GA Neurosurgical Institute, University of Ioannina, Stavros Niarchos Avenue, 45500 Ioannina, - - 2014
(99m)Tc-Tetrofosmin ((99m)Tc-TF) and (99m)Tc-Sestamibi ((99m)Tc-MIBI) are SPECT tracers that have been used for brain tumor imaging. Tumor's multidrug resistance phenotype, namely, P-glycoprotein (p-gp), and the multidrug resistance related proteins (MRPs) expression have been suggested to influence both tracers' uptake. In the present study we set out to compare (99m)Tc-TF and ...
- - 2014
[This corrects the article DOI: 10.1371/journal.pone.0105602.].
Zhou Ming-Da MD Micro &Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering and Materials Research Institute, Pennsylvania State University, University Park, PA 16802, - - 2014
The analysis of circulating tumour cells (CTCs) in cancer patients could provide important information for therapeutic management. Enrichment of viable CTCs could permit performance of functional analyses on CTCs to broaden understanding of metastatic disease. However, this has not been widely accomplished. Addressing this challenge, we present a separable bilayer ...
Chen Liping L Department of Traditional Chinese Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, - - 2013
The Chinese herb Shuyusan, whose main constituent is jasminoidin, has been shown to protect SH-SY5Y cells against corticosterone-induced damage. SH-SY5Y cells injured by 400 μmol/L corticosterone were treated with 5 and 30 μg/mL Shuyusan-containing serum. Results revealed that Shuyusan-containing serum elevated the survival rate of SH-SY5Y cells, reduced Bax expression, ...
Lainioti G Ch - - 2012
The technique of reversed-flow gas chromatography (RFGC) was employed for the determination of the alcoholic fermentation phases and of kinetic parameters for free and immobilized cell systems, at different initial glucose concentrations and temperature values. In addition to this, due to its considerable advantages over other techniques, RFGC was used ...
Miao Qingqing - - 2012
A radioactive probe (1) has been developed and applied to a condensation reaction and self-assembly of radioactive nanoparticles (i.e., (125)I-NPs) intracellularly. Upon 160 min cellular efflux, the radioactivity retained in cells incubated with 1 was 4-fold more higher than that of those cells treated with a scrambled control probe (1-Scr).
Wang Peng-Yuan - - 2012
Topographic cues have been recognized crucial on the modulation of cell behavior, and subsequent important for the design of implants, cell-based biomedical devices and tissue-engineered products. Grooved topography direct cells to align anisotropically on the substrates, resulting in an obvious morphological difference compared with the flat and the other topographies. ...
Carlin Viviane - - 2012
Abstract The aim of the present study was comparatively evaluate genomic damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from hairdressers using two different anatomic buccal sites: cheek mucosa and lateral border of the tongue. A total of 28 hairdressers and 30 health controls ...
Nguyen Khanh K Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, - - 2013
Currently, the most severe limitation to applying RNA interference technology is delivery, including localizing the molecules to a specific site of interest to target a specific cell population and sustaining the presentation of these molecules for a controlled period of time. In this study, we engineered a functionalized, biodegradable system ...
Sachar Ashneet - - 2012
An in-depth understanding of the interactions between cells and three-dimensional (3D) matrices (scaffolds) is pivotal to the development of novel biomaterials for tissue regeneration. However, it remains a challenge to find suitable biomimetic substrates and tools to observe cell-material and cell-cell interactions on 3D matrices. In the present study, we ...
Proksch Susanne - - 2012
When prospectively applied for regenerative therapies, human bone marrow-derived mesenchymal stem cells (hMSCs) interact with the locally residing host cells. With respect to the developmentally particular origin of oral cells, little is known about the putatively discriminative behavioural responses of hMSCs in interaction with various oral cell types, including human ...
Chen Bifeng - - 2012
A selective and sensitive fluorescence probe for hydrogen sulfide (H(2)S) detection was synthesized and evaluated in PBS buffer and fetal bovine serum. The effect of pH on the probe was also studied. In addition, visualization of H(2)S in Hela cells was achieved using confocal laser scanning fluorescence microscopy.
Zhou Rui - - 2012
A hydrated gel-like pericellular matrix (PCM) covers the surface of all eukaryotic cells and plays a key role in many cellular events, but its effect on nanoparticle internalization has not been studied. Here, using cells with various PCM thicknesses and gold nanoparticles as probes, we demonstrate that, rather than being ...
Martinez Inigo - - 2012
The exact contribution of transplanted chondrocytes for cartilage tissue repair prior expansion in monolayer cultures remains undetermined. At our laboratory we have created a new permeable chamber to study the chondrogenesis of de-differentiated cells implanted ectopically in a closed and controlled environment. The behavior of chondrocytes has been studied in ...
Madanayake T W TW Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, - - 2012
Cytochromes P450 (P450s) contribute to the metabolic activation and inactivation of various endogenous substrates. Despite years of research, the physiological role of CYP2S1 remains unknown. CYP2S1 has demonstrated NADPH P450-reductase-independent metabolism of cyclooxygenase (COX)-derived prostaglandins [e.g., prostaglandin G(2) (PGG(2))] at nanomolar concentrations. Arachidonic acid is converted to prostaglandin precursors [PGG(2) ...
Zink Christian - - 2012
Surface gradients facilitate rapid, high-throughput, systematic investigations in cell biology, materials science, and other fields. An important surface parameter is the surface roughness on both the micrometer and nanometer scales in the lateral direction. Two approaches have been combined to create two-dimensional roughness gradients by adding a nanoparticle density gradient ...
Zhou Xiongtu - - 2012
Cells display contact guidance when cultured on topographical cues. By combining standard photolithography, nanoimprint lithography, and soft lithography, we produced sophisticated patterns on two levels, including crossing microgrooves with different depth/spacing and microgrooves with superimposed submicrometer features. The results show that for narrowly spaced microgrooves, the contact guidance is more ...
Shon Won-Jun - - 2012
In regard to biological properties of endodontic sealers, there are many characteristics that should be considered. The aim of this study was to examine the biological effects of new calcium phosphate-based root canal sealers, CAPSEAL I and CAPSEAL II (CPS), on human periodontal fibroblast cells by examining the expression levels ...
Rane Tushar D TD Department of Biomedical Engineering, Johns Hopkins University, Baltimore, - - 2012
In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect ...
Sinthuvanich Chomdao - - 2012
Iterative peptide design was used to generate two peptide-based hydrogels to study the effect of network electrostatics on primary chondrocyte behavior. MAX8 and HLT2 peptides have formal charge states of +7 and +5 per monomer, respectively. These peptides undergo triggered folding and self-assembly to afford hydrogel networks having similar rheological ...
Chang M-C - - 2012
Chang MC, Tsai YL, Chen YW, Chan CP, Huang CF, Lan WC, Lin CC, Lan WH, Jeng JH. Butyrate induces reactive oxygen species production and affects cell cycle progression in human gingival fibroblasts. J Periodont Res 2012; doi: 10.1111/j.1600-0765.2012.01504.x. © 2012 John Wiley & Sons A/S Background and Objective:  Short-chain ...
Lourenço Bianca N - - 2012
Surface wettability and topography are recognized as critical factors influencing cell behavior on biomaterials. So far only few works have reported cell responses on surfaces exhibiting extreme wettability in combination with surface topography. The goal of this work is to study whether cell behavior on superhydrophobic surfaces is influenced by ...
Myochin Takuya - - 2012
Near-infrared (NIR) fluorescence probes are especially useful for simple and noninvasive in vivo imaging inside the body because of low autofluorescence and high tissue transparency in the NIR region compared with other wavelength regions. But, existing NIR fluorescence probes for matrix metalloproteinases (MMPs), which are tumor, atherosclerosis, and inflammation markers, ...
Hong Sungmin - - 2012
We have developed an integrated microfluidic material processing chip and demonstrated the rapid production of collagen microspheres encapsulating cells with high uniformity and cell viability. The chip integrated three material processing steps. Monodisperse microdroplets were generated at a microfluidic T junction between aqueous and mineral oil flows. The flow was ...
Koizumi Koji - - 2012
A key question in developmental biology is how cellular patterns are created and maintained. During the formation of the Arabidopsis root, the endodermis, middle cortex (MC), and cortex are produced by periclinal cell divisions that occur at different positions and at different times in root development. The endodermis and cortex ...
Pendegrass Catherine Jane - - 2012
Amputation places a significant burden on healthcare systems worldwide as patients suffer life-long complications associated with the stump-socket interface. Skin penetrating, osseointegrated implants like intraosseous transcutaneous amputation prostheses, could overcome this, however, they rely on the formation and maintenance of an infection-free seal at the skin-implant interface. Epithelial cell migration ...
Kim Eun Sun - - 2012
Establishing probe-based analysis is important for developing multi-photon microscopy (MPM) to make an early diagnosis of colon neoplasm and assess its antioxidant status. Cu(I) and Zn(II) ions are trace elements which roll as cofactors of antioxidant, superoxide dismutase. However, there have been no reports on the features of MPM image ...
Bilginer Sinan - - 2012
Two series of 1-(3-aminomethyl-4-hydroxyphenyl)-3-pyridinyl-2-propen-1-ones, designed as novel cytotoxins, were synthesized. The compounds had low CC(50) values in the micromolar range against HL-60 promyelocytic leukemic cells and HSC-2, HSC-3 and HSC-4 oral squamous cell carcinomas. The CC(50) values of these compounds were higher towards non-malignant HGF (gingival fibroblasts), HPC (pulp cells), ...
Smith J G - - 2012
The present study aimed to determine whether dentine tissue and preparations of extracellular matrix (ECM) from pulp (pECM) and dentine (dECM), and breakdown products, influenced pulp cell migration. Chemotaxis transwell and agarose spot assays demonstrated that both dentine and pulp ECM molecules acted as chemoattractants for primary pulp cells. Chemoattractant ...
Dowling Enda P EP Mechanical and Biomedical Engineering, National University of Ireland, University Road, Galway, Republic of - - 2012
The biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and ...
Sharma Lalit Kumar - - 2012
Probing the unknown: The immunoproteasome, an alternative form of the constitutive proteasome, has been implicated in a number of pathological states such as cancer and autoimmune diseases. In an effort to understand the role of the immunoproteasome in cells, the first immunoproteasome-specific near-infrared fluorescent probe has been developed.
1 2 3 4 5 6 7 8 9 10 >