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Chen Shicheng - - 2013
Flavobacterium hibernum, isolated from larval habitats of the eastern tree hole mosquito, Aedes triseriatus, remained suspended in the larval feeding zone much longer (8 days) than other bacteria. Autofluorescent protein (AFP) markers were developed for labeling F. hibernum using a strong flavobacterial expression system. GFP-tagged F. hibernum cells were quickly ...
Cheng Du - - 2013
In this study, we explored the growth dynamics of Rickettsia sp. phylotype G021 during transovarial transmission and transstadial passage by Ixodes pacificus using real-time quantitative PCR. Four parental engorged I. pacificus females were allowed to complete their developmental stages until the F2-generation eggs yielded unfed larvae. All eggs, larvae, nymphs, ...
Frey Eva - - 2013
The reproductive cycle of the parasitic mite Varroa destructor is closely linked to the development of the honey bee host larvae. Using a within colony approach we introduced phoretic Varroa females into brood cells of different age in order to analyze the capacity of certain stages of the honey bee ...
Karp Xantha - - 2013
The Caenorhabditis elegans vulval precursor cells (VPCs) offer a paradigm for investigating how multipotency of progenitor cells is maintained during periods of quiescence. The VPCs are born in the first larval stage. When hermaphrodites are grown under favorable conditions, the EGF-mediated "inductive" signal and the LIN-12/Notch-mediated "lateral" signal confer a ...
Buntrock Lydia - - 2012
Organ growth depends on cell division and (or) cell growth. Here, we present a study on two organs whose growth depends entirely on cell growth, once they are formed in the embryo: Malpighian tubules and silk glands of the flour moth, Ephestia kuehniella . Between first and last larval instar, ...
Aguila Jerell R - - 2012
Within the complex life cycle of holometabolous insects, nutritional resources acquired during larval feeding are utilized by the pupa and the adult. The broad features of the transfer of larval resources to the pupae and the allocation of larval resources in the adult have been described by studies measuring and ...
Koga Masaaki - - 2012
The isolated right half (RH) or left half (LH) of Xenopus embryos can undergo regulation so as to form well-proportioned larvae. To assess how the combined actions of maternal determinants and cell-cell interactions contribute to form the well-proportioned larvae, we quantitatively compared four-cell stage blastomere fate between normal larvae and ...
Kong Jaemin - - 2012
Additional post-processing techniques, such as post-thermal annealing and UV illumination, were found to be required to obtain desirable values of the cell parameters in a tandem polymer solar cell incorporated with solution-processed basic n-type titanium sub-oxide (TiO(x))/acidic p-type poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) interlayers. Subsequent to the fabrication of the tandem polymer solar ...
He Weizhen - - 2012
We used Ti meshes for both the photoanodes and counter electrodes of dye-sensitized solar cells (DSSCs) to improve the flexibility and conductivity of the electrodes. These mesh type electrodes showed good transparency and high bendability when subjected to an external force. We demonstrated the advantages of cells using such electrodes ...
Veldhuis Nicholas A - - 2012
The balance of glycosylation and deglycosylation of ion channels can markedly influence their function and regulation. However, the functional importance of glycosylation of the TRPV1 receptor, a key sensor of pain-sensing nerves, is not well understood, and whether TRPV1 is glycosylated in neurons is unclear. We report that TRPV1 is ...
Gruber Helen E - - 2012
BACKGROUND CONTEXT: Carragee et al. have recently shown that modern discography injections are associated with subsequent acceleration of disc degeneration, herniation, and loss of disc height. Although needle puncture and pressurization are known trauma events that can create disc degeneration in animal models, another likely culprit in clinical discography-associated degeneration is ...
Palanchoke Ujwol - - 2012
Plasmonic effects in amorphous silicon thin film solar cells with randomly textured metal back contact were investigated experimentally and numerically. The influence of different metal back contacts with and without ZnO interlayer was studied and losses in the individual layers of the solar cell were quantified. The amorphous silicon thin ...
Sitte Ingrid - - 2012
The basis of disc degeneration is still unknown, but is believed to be a cell-mediated process. Apoptosis might play a major role in degenerative disc disease (DDD). The aim of this study was to correlate the viability of disc cells with the radiological degeneration grades (rDG) in disc herniation. Forty ...
Ciapetti Gabriela - - 2012
Disc degeneration, and associated low back pain, are a primary cause of disability. Disc degeneration is characterized by dysfunctional cells and loss of proteoglycans: since intervertebral tissue has a limited capacity to regenerate, this process is at present considered irreversible. Recently, cell therapy has been suggested to provide more successful ...
Ping Liyan - - 2012
The motion of small bacteria consists of two phases: relatively long runs alternate with intermittent stops, back-ups, or tumbles, depending on the species. In polar monotrichous bacteria, the flagellum is anchored at the cell pole inherited from the parent generation (old pole) and is surrounded by a chemoreceptor cluster. During ...
Kang Soo Kyung - - 2012
Aims: The fate decision of adult stem cells is determined by the activation of specific intracellular signaling pathways following exposure to specific stimuli. In this study, we demonstrated specific functions of a novel small molecule, CBM-1078, that induced cell self-renewal via Oct4- and canonical Wnt/β-catenin-mediated deaging in cultured hATSCs. Results: ...
Ydenberg Casey A CA Department of Molecular Biology, Princeton University, Princeton, NJ 08544, - - 2012
Cell fusion is the key event of fertilization that gives rise to the diploid zygote and is a nearly universal aspect of eukaryotic biology. In the yeast Saccharomyces cerevisiae, several mutants have been identified that are defective for cell fusion, and yet the molecular mechanism of this process remains obscure. ...
Driscoll Meghan K - - 2012
We observe and quantify wave-like characteristics of amoeboid migration. Using the amoeba Dictyostelium discoideum, a model system for the study of chemotaxis, we demonstrate that cell shape changes in a wave-like manner. Cells have regions of high boundary curvature that propagate from the leading edge toward the back, usually along ...
Whitley Derick B - - 2011
A 5-year-old, spayed female boxer dog presented to the referring veterinarian with a year-long history of swelling, ulceration and pain in the pawpad of the fourth digit of the right forelimb. Histologically, the pawpad was expanded by a mass composed of small polygonal cells forming broad bands and trabeculae within ...
Zur Lage Petra I - - 2011
Much of developmental biology is concerned with the processes by which cells become committed to particular fates in a regulated fashion, whereas cell biology addresses, among other things, the variety of differentiated forms and functions that cells can acquire. One open question is how the regulators of the former process ...
Baghbaderani Behnam A - - 2011
Tissue-specific human neural precursor cells (hNPCs) can be isolated from various regions of the developing or adult central nervous system and may serve as a viable source of cells in cell replacement therapies for the treatment of neurodegenerative disorders. However, in order for cell replacement strategies to become a routine ...
Eiriz Maria Francisca - - 2011
Neurogenesis in the adult mammalian brain occurs in two specific brain areas, the subventricular zone (SVZ) bordering the lateral ventricles and the subgranular zone (SGZ) of the hippocampus. Although these regions are prone to produce new neurons, cultured cells from these neurogenic niches tend to be mixed cultures, containing both ...
McClain Justin A - - 2011
Binge alcohol exposure in adolescent rats potently inhibits adult hippocampal neurogenesis by altering neural progenitor cell (NPC) proliferation and survival; however, it is not clear whether alcohol results in an increase or decrease in net proliferation. Thus, the effects of alcohol on hippocampal NPC cell cycle phase distribution and kinetics ...
Schmidt Manfred M Neuroscience Institute and Department of Biology, Georgia State University, Atlanta, Georgia 30302-5030, USA. - - 2011
New interneurons are continuously generated in small proliferation zones within neuronal somata clusters in the olfactory deutocerebrum of adult decapod crustaceans. Each proliferation zone is connected to a clump of cells containing one neural stem cell (i.e., adult neuroblast), thus forming a "neurogenic complex." Here we provide a detailed analysis ...
Afzal Elahe - - 2011
HSPs (heat shock proteins) have been recognized to maintain cellular homoeostasis during changes in microenvironment. The present study aimed to investigate the HSPs expression pattern in hierarchical neural differentiation stages from mouse embryonal carcinoma stem cells (P19) and its role in heat stressed exposed cells. For induction of HSPs, cells ...
Jin Guang-Zhen - - 2011
Neurite outgrowth from endogenous or transplanted cells is important for neural regeneration following nerve tissue injury. Modified substrates often provide better environments for cell adhesion and neurite outgrowth. This study was conducted to determine if MWCNT (multiwalled carbon nanotube)-coated electrospun PLCL [poly (l-lactic acid-co-3-caprolactone)] nanofibres improved the neurite outgrowth of ...
Liu Qian - - 2011
Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuclear cells into retina-like cells and integration into the ...
Connor Bronwen - - 2011
The presence of both neural and glial precursor cells in the adult central nervous system (CNS) and the capacity of these cells to migrate through this mature structure to areas of pathological damage and injury raises hope for the development of new therapeutic strategies to treat brain injury and disease. ...
Lai Wing Sun Karen - - 2011
Netrins are secreted proteins that were first identified as guidance cues, directing cell and axon migration during neural development. Subsequent findings have demonstrated that netrins can influence the formation of multiple tissues, including the vasculature, lung, pancreas, muscle and mammary gland, by mediating cell migration, cell-cell interactions and cell-extracellular matrix ...
Widera Darius - - 2011
Adult neural crest related-stem cells persist in adulthood, making them an ideal and easily accessible source of multipotent cells for potential clinical use. Recently, we reported the presence of neural crest-related stem cells within adult palatal ridges, thus raising the question of their localization in their endogenous niche. Using immunocytochemistry, ...
Gao Yandong Y Department of Mechanical Engineering, Vanderbilt University, Nashville, TN 37235, - - 2011
A versatile microfluidic platform allowing co-culture of multiple cell populations in close proximity with separate control of their microenvironments would be extremely valuable for many biological applications. Here, we report a simple and compact microfluidic platform that has these desirable features and allows for real-time, live-cell imaging of cell-cell interactions. ...
Garbayo Elisa - - 2011
Cell-based therapies for global cerebral ischemia represent promising approaches for neuronal damage prevention and tissue repair promotion. We examined the potential of marrow-isolated adult multilineage-inducible (MIAMI) cells, a homogeneous subpopulation of immature human mesenchymal stromal cell, injected into the hippocampus to prevent neuronal damage induced by global ischemia using rat ...
Baas Peter W - - 2011
New evidence suggests that katanin - best known for severing microtubules in their more stable regions - localizes at the leading edge of migratory cells and trims microtubules at their dynamic plus ends.
Santra Manoranjan - - 2011
Analysis of microarray probe data from glioma patient samples, in conjunction with patient Kaplan-Meier survival plots, indicates that expression of a glioma suppressor gene doublecortin (DCX) favors glioma patient survival. From neurosphere formation in culture, time-lapse microscopic video recording, and tumor xenograft, we show that DCX synthesis significantly reduces self-renewal ...
Kilander Michaela Bc - - 2011
Aim:  The objective of this study is to compare the efficacy of different recombinant, commercially available WNTs with regard to WNT/β-catenin signaling, Dishevelled (DVL) and G protein activation and the induction of cell proliferation in a microglia-like cell line called N13. Method:  For detection of activated signaling molecules, cell lysates ...
Zheng Kang - - 2012
Mounting evidence indicates that microRNAs (miRNAs) play important roles in the control of glial cell development in the central nervous system. Suppression of miRNA formation disrupts the initial generation of oligodendrocyte progenitor cells from the ventricular neuroprogenitor cells in the embryonic spinal cord. miRNAs also regulate the later events of ...
Lee Choong Hyun - - 2011
Newly generated neurons in the dentate gyrus differentiate into mature granule cells. In the present study, we observed the effects of adrenalectomy (ADX) and corticosterone replacement therapy (CRT) on cell death, cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus (SZDG). For this, the animals ...
Wellik Deneen M - - 2011
The adult mammalian kidney is generated by the differentiation and integration of several distinct cell types, including the nephrogenic mesenchyme, ureteric epithelium, stromal and endothelial cells. How and where these cell types are generated and what signals lead to their differentiation and integration into a functional organ system is a ...
Raso Giuseppina Mattace - - 2011
Palmitoylethanolamide (PEA) regulates many pathophysiological processes in the CNS, including pain perception, convulsions, and neurotoxicity and increasing evidence points to its neuroprotective action. Here, we report that PEA, acting as a ligand of peroxisome-proliferator activated receptor (PPAR)-α, might regulate neurosteroidogenesis in astrocytes, which, as other glial cells and neurons, have ...
Loewenbrück Kai F - - 2011
Abstract Recently, a peak at 1.28 ppm in proton magnetic resonance spectroscopy ((1)H-MRS) of neural stem cells (NSCs) was introduced as a noninterventional biomarker for neurogenesis in vivo. This would be an urgently needed requisite for translational studies in humans regarding the beneficial role of adult neurogenesis for the structural and ...
Forni Paolo Emanuele - - 2011
The origin of GnRH-1 cells and olfactory ensheathing cells has been controversial. Genetic Cre-lox lineage tracing of the neural crest (NC) versus ectodermal contribution to the developing nasal placode was performed using two complementary mouse models, the NC-specific Wnt1Cre mouse line and an ectodermal-specific Crect mouse line. Using these lines ...
Eloqayli Haytham - - 2011
This study was undertaken to determine if the ketogenic diet could be useful for glioblastoma patients. The hypothesis tested was whether glioblastoma cells can metabolize ketone bodies. Cerebellar astrocytes and C6 glioblastoma cells were incubated in glutamine and serum free medium containing [2,4-(13)C]β-hydroxybutyrate (BHB) with and without glucose. Furthermore, C6 ...
Iida Atsumi - - 2011
Much attention has been paid to the roles of microRNA in developmental and biological processes. Dicer plays essential roles in cell survival and proliferation in various organs. We examined the role of Dicer in retinal development using retina-specific conditional knockout of Dicer in mice. Dkk3-Cre expressed the Cre gene in ...
Feng Ya-Bo - - 2011
Diffuse brain injury (DBI) has been shown to increase the proliferation of granule cell precursors in the adult dentate gyrus (DG). However, the mechanism by which DBI-induced cell proliferation in the DG may enhance seizure susceptibility remains largely unknown. Using bromodeoxyuridine (BrdU) immunohistochemistry, we examined the effects of group II ...
Bae Keum Seok - - 2011
Mesenchymal stem cells (MSCs) are multipotent and give rise to distinctly differentiated cells from all three germ layers. Neuronal differentiation of MSC has great potential for cellular therapy. We examined whether the cluster of mechanically made, not neurosphere, could be differentiated into neuron-like cells by growth factors, such as epidermal ...
Palomo Gloria M - - 2011
Friedreich's ataxia is an autosomal recessive disease caused by mutations that produce a deficiency in frataxin. Despite of the importance of neurodegeneration in Friedreich's ataxia, little is known about the consequences of frataxin deficit in neuronal cells. Here we describe a neuronal cell model for Friedreich's ataxia based on the ...
Hu Linyan - - 2011
AIM OF THE STUDY: The present study was aimed to explore the effects of Salvia miltorrhiza in inducing rMSCs to differentiate into functional neurons. MATERIALS AND METHODS: rMSCs were isolated and cultured in vitro, then Salvia miltorrhiza was added to induce rMSCs to differentiate repeatedly for 5 times with an ...
Tanaka Daisuke H - - 2011
The establishment of the mammalian neocortex is often explained phylogenetically by an evolutionary change in the pallial neuronal progenitors of excitatory projection neurons. It remains unclear, however, whether and how the evolutionary change in inhibitory interneurons, which originate outside the neocortex, has been involved in the establishment of the neocortex. ...
Pavlov Anton M - - 2011
Neuron cells uptake of biodegradable and synthetic polymeric microcapsules functionalized with aggregates of gold nanoparticles incorporated into their shells is demonstrated in situ. In addition to traditionally used optical microscopy, electron microscopy is used both for higher-resolution imaging and for confirming the uptake by focused ion beam cross-sectioning of specific ...
Lange Annika - - 2011
BACKGROUND: Small cell lung cancer (SCLC) is one of the most aggressive malignancies implying a very poor prognosis for patients even under therapy. Since it is known that SCLC cells exhibit neurone-like characteristics, we investigated whether a neuronal induction medium (NID) consisting of indomethacin (200μM), 3-isobutyl-1-methylxanthine (IBMX, 500μM) and insulin ...
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