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Results 951 - 983 of 983
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Ray B - - 1973
More than 90% of the surviving cells of Escherichia coli NCSM were injured after freezing in water at -78 C. Injury was determined by the ability of cells to form colonies on Trypticase soy agar with yeast extract but not on violet red-bile agar and deoxycholate-lactose agar. Exposure of the ...
Mond J J - - 1972
Spleen cells from LAF(1) mice hyperimmune to sheep erythrocytes (SE) lost their ability to transfer a secondary response to irradiated recipients after incubation with anti-theta and rabbit complement in vitro. Small numbers of specific immune cells even when taken 3 days after a primary SE injection reconstituted the direct and ...
Ray B - - 1972
Freezing an aqueous suspension of Escherichia coli NCSM at -78 C for 10 min, followed by thawing in water at 8 C for 30 min, resulted in the death of approximately 50% of the cells, as determined by their inability to form colonies on Trypticase soy agar containing 0.3% yeast ...
Plattner H - - 1972
The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed ...
Ray B - - 1972
Fast freezing and slow thawing of Salmonella anatum cells suspended in water resulted in injury of more than 90% of the cells that survived the treatment. The injured cells failed to form colonies on the selective medium (xyloselysine-peptone-agar with 0.2% sodium deoxycholate) but did form colonies on a nonselective (xylose-lysine-peptone-agar) ...
Chui D H - - 1971
The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined. An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures. The rate of protein synthesis by individual ...
Ray B - - 1971
Repair of injury induced by freeze-drying Salmonella anatum in nonfat milk solids occurred rapidly after rehydration. Injury in surviving cells was defined as the inability to form colonies on a plating medium containing deoxycholate. Death was defined as inability to form colonies in the same medium without this selective agent. ...
Maniatis G M - - 1971
Rabbit antibodies specific for the major tadpole and frog hemoglobin components of R. catesbeiana were used for the detection of the two hemoglobins inside single cells. The antisera, after fractionation by ammonium sulfate precipitation and diethylaminoethyl (DEAE)-cellulose chromatography, were conjugated with fluorescein isothiocyanate for the antifrog hemoglobin serum and tetramethylrhodamine ...
Navidi M H - - 1970
An inorganic liquid photovoltaic cell is described. The cell is based on the reaction 2Mo(4+) <--> Mo(5+) + Mo(3+), with pentavalent molybdenum formed in the illuminated half-cell and trivalent molybdenum formed in the dark half-cell. In the photochemical reaction pentavalent molybdenum precipitates. Consequently, the cell has the capability of storing ...
Rozee K R - - 1970
A continuous cell line of rhesus monkey kidney cells, LLC-MK(2), was infected with reovirus type 1 (Lang). The cells were freeze-etched as a method for observing the structural details of the reovirus-induced cytoplasmic inclusion. Information on maturation may be obtained by preparing infected cells for electron microscopy by freeze-etching.
Sinskey T J - - 1970
When cells of Escherichia coli ML30 were suspended in 2% gelatin and frozen at -40 C, no appreciable metabolic damage or death occurred. After freeze-drying for 8 hr at a platen temperature of 49 C and rehydration with a mineral salts medium, survival of the cells was 0.6%. Metabolic damage ...
Sorrells K M - - 1970
Freezing (-75 C) and storage (-20 C) of a cell suspension of Salmonella gallinarum resulted in a heterogeneous population of dead, metabolically injured, and unharmed cells. Injured cells constituted as much as 40% of those surviving freezing and storage for 1 day. Replica plating of frozen and thawed cells indicated ...
Bertles J F - - 1968
THE AMOUNT OF FETAL HEMOGLOBIN (HB F) IN ERYTHROCYTES OF PATIENTS WITH SICKLE CELL ANEMIA (HB SS DISEASE) WAS MEASURED BY TWO METHODS: (a) photometry of individual cells stained for Hb F by the Kleihauer-Betke technique; and (b) chemical assay of alkali-resistant hemoglobin in cells distributed according to specific gravity ...
Murphy J R - - 1968
Suspensions of erythrocytes from patients with hemoglobin (Hb) CC disease showed an increased viscosity and decreased filterability suggesting a less deformable cell. Hemolysates prepared from Hb CC erythrocytes had an increased viscosity compared with hemolysates of normal cells, suggesting that the increased viscosity of Hb CC cells in serum was ...
Heckly R J - - 1967
Various materials were mixed with suspensions of Serratia marcescens and other organisms. Samples were removed and frozen at intervals after mixing; the number of cells that survived both freeze-drying and exposure to air varied rhythmically as a function of time between mixing and freezing. When assayed before or immediately after ...
Chien S - - 1967
Suspensions of canine and human erythocytes hardened with acetaldehyde differ from the suspensions of normal erythrocytes with respect to their rheological behavior. Normal erythrocytes can be packed by centrifugation so that the sediment volume is nearly 100 percent cells, but the hardened erythrocytes (RBC's) can be packed only to approximately ...
MacLeod R A - - 1967
When distilled water from a tin-lined still served as the plating diluent, cells of Aerobacter aerogenes developed symptoms of metabolic injury as evidenced by increased counts on supplemented, as compared with minimal, plating medium. Cysteine was as effective as yeast extract as a supplement to the minimal medium in increasing ...
Jensen W N - - 1967
Increased mechanical fragility of the sickled red cell is thought to be important in the genesis of the hemolytic process in sickle-cell disease. Sickled cells were observed cinematographically after microincision by a ruby-laser beam. Distortion and charring invariably occurred at the site of injury, and with small injuries there was ...
Lingg A J - - 1967
The per cent viability of Bacillus popilliae after lyophilization of liquid nitrogen frozen cells was determined. Lyophilization of 9- to 12-hr cells which had been suspended in 5% sodium glutamate plus 0.5% gum tragacanth, frozen in liquid nitrogen vapor, and dried 4 to 5 hr with the ampoules exposed to ...
Wolin M J - - 1966
Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m NaCl or KCl or 0.3 m sucrose. ...
Moss C W - - 1966
Moss, C. Wayne (North Carolina State University, Raleigh), and M. L. Speck. Release of biologically active peptides from Escherichia coli at subzero temperatures. J. Bacteriol. 91:1105-1111. 1966.-Freezing and storage of Escherichia coli at -20 C in phosphate buffer resulted in loss of cell viability and a pronounced leakage of cellular ...
VALU J A - - 1965
Since washed cells of Lactobacillus leichmannii ATCC 7830 frequently showed erratic results in vitamin B(12) assays, a depletion technique was used to stabilize the inoculum. The method consisted of incubating the twice-washed cells at 37 C for 45 min in distilled water. Survival and growth studies indicated that the stabilizing ...
MARKS P A - - 1964
Rates of synthesis of hemoglobin A by erythroid cells from thalassemic subjects are markedly decreased. Formation of hemoglobin F, however, proceeds at similar rates in cells from subjects with thalassemia and other types of hemolytic anemias. A mechanism is suggested regarding the altered patterns of hemoglobin synthesis under conditions of ...
DANNENBERG A M AM - - 1964
Oil-induced mononuclear phagocytes (MN) were quantitatively assayed for various hydrolases as unfractionated suspensions of frozen and thawed cells. They apparently contain two proteases. The first, measured with urea- or acid-denatured hemoglobin, was similar to purified Proteinase I of lung with respect to pH optimum (pH 4), stability, hydrolytic and polymerizing ...
SURDY T E - - 1963
Surdy, Theodore E. (Purdue University, Lafayette, Ind.) and S. E. Hartsell. Correlation of speciation with lytic responses of the Achromobacter. J. Bacteriol. 85:1011-1016. 1963.-Lysozymic lysis of six species of Achromobacter was investigated. Three of the six species were lysed with 33, 50, or 100 mug/ml of lysozyme; if higher concentrations ...
ZIMMERMAN L - - 1962
Zimmerman, Leonard (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Survival of Serratia marcescens after freeze-drying or aerosolization at unfavorable humidity. I. Effects of sugars. J. Bacteriol. 84:1297-1302. 1962.-Suspensions of Serratia marcescens were subjected to freeze-drying or to aerosolization at unfavorable humidity levels. The survival of the cells during one ...
SAGERS R D - - 1962
An easily constructed, highly efficient cell homogenizing press is described which should be available to laboratories at moderate cost. The press allows the preparation of homogenates from up to 20 g of packed cell paste in a single batch without danger of damage to the press. Cell breakage and recovery ...
NAKAMURA M - - 1962
Shigella sonnei was frozen at -20 C in saline, nutrient broth, and milk, and plated, after thawing, upon synthetic medium, nutrient agar, and blood heart infusion agar. There was a difference in the numbers of cells recovered when the frozen and thawed cells were grown on different media. The synthetic ...
MAZUR P - - 1961
Mazur, Peter (Oak Ridge National Laboratory, Oak Ridge, Tenn.). Manifestations of injury in yeast cells exposed to subzero temperatures. I. Morphological changes in freeze-substituted and in "frozen-thawed" cells. J. Bacteriol. 82:662-672. 1961.-When cells of the yeast Saccharomyces cerevisiae are cooled rapidly to -30 C or below, fewer than 0.01% survive. ...
BAAR S - - 1960
Alkali denaturation of the haemoglobin derived from burned patients at various intervals from the time of the injury was investigated. A delayed denaturation curve was always found. The involvement of the transfused cells was established by a differential agglutination technique. Red cell populations were fractionated into two portions, one of ...
ATWOOD K C - - 1959
The number of cells that remain free in the presence of agglutinin is usually much larger than the number of inagglutinable cells. The true inagglutinable proportion can be found by successive agglutinations of a labeled population in the presence of unlabeled carrier cells. By this means it is shown that ...
APPELBOOM J W - - 1956
The freezing point depression of freshly excised frozen tissues, pulverized in a hydraulic press or in a mortar, is greater than that of plasma. Even at 0 degrees C. the freezing point depression of such homogenates increases significantly with time. Dilution data indicate that such freezing point data are valid. ...
Jaffe H L - - 1923
1. Moderate or marked ovarian enlargement has been observed in 76 per cent of rabbits which survived double suprarenalectomy over 30 days. 2. The ovarian enlargement consists essentially of an hypertrophy of the interstitial cells. 3. The hypertrophy of the interstitial cells is a compensatory reaction and adds further data ...
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