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Dooley D C - - 1981
Clinical investigations have shown that the transfusion of human granulocytes can protect the neutropenic patient against acute bacterial infection. The increasing utilization of granulocyte therapy plus an inability to store these cells in liquid media have created a requirement for a cryopreservation technique. However, the cryopreservation of human granulocytes has ...
Nash A A AA Department of Pathology, University of Cambridge, Cambridge, - - 1981
In this review Tony Nash and Philip Gell consider mainly recent work on the delayed hypersensitivity (DH) T cell (TDH) in mice, using viruses as infectious agents to probe the nature of DH and its relationship to protection.
Stephens E A - - 1981
Conditions were standardized for optimum cryopreservation of avian lymphoid cells. Several factors that influenced cryopreservation were examined. The optimum procedure was as follows: a maximum of 50 x 10(6) cells/ml were suspended in the freezing medium, which contained 5-10% dimethyl sulfoxide (DMSO), and the cell suspension was frozen slowly at ...
Vladutiu G D - - 1981
Fibroblasts derived from patients with I-cell disease have been shown to accumulate many natural substrates including a three to fourfold increase in sialic acid content compared to that found in normal fibroblasts. This diverse accumulation of storage material is due to a massive deficiency of multiple lysosomal hydrolases as they ...
Kondo H - - 1981
The viability and the recovery (cell attachment to the dish) after thawing of human diploid fibroblasts (TIG-1) frozen by four different methods were studied at different passages. Improved results were observed in a medium of 30% fetal bovine serum plus 15% glycerol, compared with the conventional medium which contained 10% ...
Meyer L B - - 1981
Whereas Salmonella typhimurium 7136 will not grow at reduced water activity (aw), it was survival in such items as intermediate-moisture foods is of interest. Initial studies demonstrated that the addition of 0.3 M acetate (pH 4.7) to glycerol-Trypticase soy broth (BBL Microbiology Systems) solutions (aw 0.86) reduced the viability of ...
Singh J - - 1981
A method is described for the release of large quantities of mesophyll cells from leaves of both cold-hardened and nonhardened winter rye seedlings by a combination of enzymic and physical maceration. Such preparations usually contain a certain percentage of nonviable cells and are thus not suitable for quantitative biochemical studies. ...
Burke J M - - 1981
Rabbits were examined at intervals to 90 days after receiving two or three intravitreal injections, on consecutive days, of homologous hemoglobin or saline. Cell proliferation in the vitreous was assessed by scintillation counting and radioautography after intravitreal administration of 3H-thymidine 4 hr prior to sacrifice. Two populations of vitreous cells ...
Dean A - - 1981
Twenty micromolar hemin causes no change in the rate of division of K562 cells but results in accumulation of 11 to 14 picograms of embryonic and fetal hemoglobins per cell. This effect is reversible, and hemoglobin induction in response to hemin, and loss of hemoglobin upon removal of hemin, can ...
Laiho K - - 1981
The viability of white blood cells, spermatozoa of the epididymis, cells from minced spleen, lymph node and lung, and cells aspirated from the bone marrow of the sternum was studied using the vital dye exclusion test. The material comprised 123 medicolegal autopsy cadavers which had been stored in a mortuary ...
Fuhr J E - - 1981
Cells of the original human myelogenous leukemia cell line, K 562 were induced to synthesize hemoglobin by incubation with 0.05 mM hemin. Analysis by isoelectric focusing indicated major bands in the region of hemoglobin F, Bart's hemoglobin, and embryonics. Cytochemical analysis by the Lepehene peroxidase reaction for hemoglobin indicated an ...
Fukuda M - - 1981
Erythrocyte surface glycoproteins from patients with various types of sickle cell anemia have been analyzed and compared with those from normal individuals. By hemagglutination with various anti-carbohydrate antibodies, sickle cells showed profound increase of i antigens and moderate increase of GlcNAc beta 1 leads to 3Gal beta 1 leads to ...
Stumpf W E - - 1981
A procedure for the quantitative assessment of nuclear receptors for steroid hormones--and other substances--in individual cells is presented. Thaw-mount autoradiography, a procedure developed earlier in our laboratory, is utilized. The silver grain yield (specific activity) is 16.6 disintegrations per silver as determined fo tritium in guinea pig uterine tissues. An ...
Guerrasio A - - 1981
K562 cell line was grown in liquid suspension and in plasma clot cultures. Morphological studies revealed the presence of a minority of cells, which were identified as erythroblasts. However, the majority of the cells remained unidentified. Biochemical studies confirmed the synthesis of hemoglobin by K562 cells. The pattern of hemoglobin ...
Wong S C - - 1981
A trimodal distribution of the amounts of Hb S in 56 sickle cell heterozygotes was observed. The variable amounts of Hb S were directly related to the mean cell volume and the mean cell Hb and were a result of the difference in net synthesis of Hb S due to ...
Robinson J M - - 1980
A mouse cell line (LM), which grows predominantly as spindle-shaped cells with numerous filopodia, was employed in this study. These filopodial projections appear to be important as sites of attachment to the substratum in LM cells. Morphologically the filopodia are slender projections from the cell body which usually attach to ...
Lew V L - - 1980
Simultaneous measurements of Ca content and 42K+ influx in sickle cell anaemia red cells confirm predictions from earlier data in the literature that the increased Ca content of sickle cell anaemia cells which are not metabolically depleted does not cause a quinine-sensitive increase in K+ permeability. It is shown that ...
Plunkett R W - - 1980
Cells coated with complement components (C3b-C4b and C4b cells) were prepared by various methods and stored in liquid nitrogen using a low-glycerol, rapid freeze technique. Freshly coated cells, and cells coated and then frozen were tested versus various antiglobulin and anticomplement reagents to evaluate the reactivity of such frozen, stored, ...
Dzandu J K - - 1980
Membrane protein phosphorylation was measured in intact normal and sickle erythrocytes, by determining the incorporation of 32Pi. The general pattern of radiolabeling is similar for normal and sickle cells, but sickle erythrocytes incorporate approximately twice as much 32P as normal cells, and the detailed distribution of label is altered. Sickle ...
Ruusuvaara P - - 1980
This paper evaluates the numbers of corneal endothelial cells that survive transplantation. The donor endothelium was photographed with a specular microscope both before enucleation and in situ after keratoplasty. The cell density was measured in 16 corneas from donors with choroidal melanoma. Of these donor corneas 12 were cryopreserved, two ...
Powars D R - - 1980
Persons with sickle cell anemia who have elevated fetal hemoglobin or lowered erythrocyte mean corpuscular volume are reputed to have less severe clinical manifestations and a greater probability of survival. This study examines the relationship between seven clinical indicators of morbidity in sickle cell anemia and seven hematological parameters that ...
Eliason J F - - 1980
We have studied hemoglobin synthesis associated with erythroid bursts under various conditions. The slope of the curve relating log cell number to log hemoglobin synthesis is consistently greater than the slope of the curve relating log cell number to log bursts, suggesting that cellular interactions may be involved in maximal ...
Cooper A J - - 1980
The freeze-thaw method of gradient generation, using Percoll as the density medium, is shown to be adaptable for the isopycnic sedimentation of live whole cells. Methods of controlling gradient profiles are examined with preliminary evidence showing that three types of cells brought to isopycnic equilibrium in these gradients and recovered ...
Orringer E P - - 1980
The incubation of red blood cells in high concentrations of sodium bicarbonate produces a net influx of salt and water resulting in the dilution of cell hemoglobin. After reinfusion into the donor, cells swollen in this manner can be readily identified in peripheral blood samples by their low density on ...
Linton R W - - 1980
The application of ion microanalysis (IMA) to the chemical characterization of freeze-fixed, freeze-dried cells is reviewed. Particular emphasis is given to pathological studies involving the determination of the chemical composition of isolated cells (e.g. rabbit alveolar macrophages--RAMs) exposed in vitro to toxic species (e.g. Pb3O4 particles). Ion microscopic results indicated ...
Bald W B - - 1979
An explicit numerical finite difference scheme for defining the thermal history of a cell or group of cells contained within a biological sample of prescribed shape is described. Solutions are presented for three different cases of freezing of biological materials prior to microscopic examination. It is concluded that a surface ...
Getz D - - 1979
When Lettree cells are exposed to Mn2+, the cation becomes associated with cells in two ways: in a relatively loose and mobile manner that gives a six-line EPR spectrum designated Mnb*, and in an immobile, relatively tight manner that gives no detectable EPR specrtum, designated Mnb. Mnb* is probably on ...
Rifkind S - - 1979
An intermittent-flow blood cell separator was used to perform a sub-total RBC exchange pheresis with prompt relief or priapism secondary to sickle cell disease. The blood cell separator offers an efficient, practical, safe method of performing exchange transfusion in the adult. Surgical procedures in the treatment of priapism have met ...
Westerman M P - - 1979
Cholesterol, phospholipid and sialic acid were measured in red cells from patients with sickle cell anemia to determine whether the cells had abnormal concentrations of these components and whether the amounts of these compounds differed in irreversibly sickled cells as compared to non-irreversibly sickled cells. Sickle cells had significantly higher ...
Friedman M J - - 1979
The host cell competence of hemoglobin C (HbC)-containing erythrocytes for Plasmodium falciparum was studied by in vitro culture. HbC homozygous red cells did not support the growth of the intracellular parasite. Heterozygous cells, however, were competent. In addition, HbC increased the resistance of sicle cell hemoglobin (HbS) red cells when ...
Boyle M D - - 1979
The inhibitory effects of 0.1 M EDTA on the lysis of E prepared by incubating EA with whole GPC was studied. At high end point lysis (greater than 70%) 0.1 M EDTA failed to prevent hemoglobin release whereas at lower end point (less than 60%) 0.1 M EDTA was effective. ...
Maniatis A - - 1979
In a group of disease states that includes sickle cell anemia (SS disease), two fetal erythrocyte markers, Hb F and i antigen, persist into adulthood. Using the technique of single-cell immunofluorescence, we determined the expression of l-i antigens and the presence of Hb F within populations of erythrocytes. Subjects tested ...
Harper P A - - 1979
Friend erythroleukemic cells can be induced by a variety of agents to synthesize hemoglobin and to exhibit other characteristics suggesting erythroid maturation. Upon induction of hemoglobin synthesis with dimethylsulfoxide (DMSO), the chloride flux in Friend cells gradually increases, until after five days of exposure to DMSO (when the hemoglobin content ...
Helpap B - - 1979
In order to evaluate a possible primary or secondary immunologic response of the spleen after single or repeated in situ freezing of parenchymal organs such as liver and kidney within a four week period, light microscopic and cell kinetic investigations with tritiated thymidine were performed on spleens of non-germfree rats. ...
Ward P C - - 1979
Occasional nonspecific poikilocytes are found in most normal blood smears, but dominance of one or more forms of poikilocytes usually is indicative of a specific anemia or disease in an organ or organ system. For example, stomatocytes are most commonly associated with acute alcoholism, while teardrop cells classically reflect "spent" ...
Finkle B J - - 1979
Cryoprotection of suspension cultures of sugarcane cells (Saccharum sp.) during freezing to various temperatures was tested using glucose, dimethylsulfoxide, and ethylene glycol at various concentrations, alone and in combinations. Viability of the thawed cells was assessed by triphenyltetrazolium chloride reduction, cell growth, and microscopic examination. Enhanced cryoprotection-as much as a ...
Dover G J - - 1979
Serial microscopic immunodiffusion assays of F cells, i.e., erythrocytes that contain fetal hemoglobin (HbF), in four individuals recovering from anemia demonstrate initial increases in the percentage of circulating reticulocytes that contain HbF (F reticulocytes) and subsequent increases in the percentage of mature erythrocytes that contain HbF (F erythrocytes). In one ...
Biagini G - - 1979
The problem of cell survival as well as the biochemical and histological effects on skin of low temperature storage in liquid nitrogen has already received attention. However, little is known of the submicroscopic alterations induced by such treatment. Our study shows that rapid or slow freezing, direct or mediate contact ...
Morris D R - - 1979
Fresh adult human erythrocytes were suspended in isotonic pH adjusted solutions containing various concentrations of Dextran T.500. The cells were subjected to uniform hydrodynamic shear stress in a Ferranti Shirley Cone and Plate Viscosimeter. The amount of lysis incurred at any given combination of exposure parameters was markedly affected by ...
Menon M - - 1979
Use of DEAE-dextran (a polycation) increases and stabilizes the rosettes formed between sheep red cells and human peripheral blood lymphocytes. Under its influence, reproducible stable rosettes are formed after one hour of incubation in an ice bath instead of the usually required 18 to 24 hours. We have shown that ...
Malasková V - - 1979
Fine structure of lymphoblastoid cell lines (LCL) derived from leukocytes of three patients with acute myelosis and one patient with undifferentiated acute leukemia were studied repeatedly by electron microscope. The majority of cells in all lines was of the same primitive lymphoblastic type as refered to in literature regardless to ...
Myagkaya G - - 1979
In trophoblastic epithelial cells of the sheep placenta the breakdown of erythrocytes within complex erythrolysosomes was studied at the ultrastructural level. It was found that the formation of complex erythrolysosomes containing from two to several erythrocytes as a result of fusion of erythrolysosomes within the epithelial cells was a common ...
Benedict R W - - 1979
A procedure was developed for attaching erythrocytes to various plastics. Plastic disks were exposed to ammonia glow discharge (AGD) and found thereafter to form weak bonds with red cells settling from a dilute saline suspension poured over the disks. When the disks were used to produce a rotating shear flow, ...
Delfini C - - 1979
Normal and pathological lymphoid cells, collected from different sources, were cryopreserved using a programmed freezing procedure. With this cryopreservation technique, the percentage of T and B cell surface markers and the proliferative response to mitogens were not influenced by 21 and 35 days of storage in liquid nitrogen. The recovery ...
van Lambalgen R - - 1979
The conditions for cryopreservation and reconstitution after thawing of cytotoxic effector cells for cell mediated lympholysis (CML) tests are studied. Percentage recovery of functional activity is analysed not by the commonly used method of comparisons at a single concentration of effector cells but by the movement of the curve of ...
Glader B E - - 1978
Cation composition, cellular hydration, and adenosinetriphosphate (ATP) content were measured in irreversible sickle cells (ISC's) separated from the blood of patients with sickle cell anaemia. Total monovalent cation (Na+ + K+) content was markedly reduced in ISC's and this largely was due to cell K+ depletion. Corresponding to the reduced ...
Fabia F - - 1978
Human red blood cells can bind in vitro to autologous peripheral blood lymphocytes forming auto-rosettes. The percentage of rosett-forming cells (A-RFC) depends on erythrocyte ageing in vivo. Old untreated cells given an A-RFC percentage lower as compared with the young ones. The same age groups of cells treated with neuraminidase ...
Will P C - - 1978
Methadone induced lysis of human erythrocytes and mouse leukemic cells was studied. The cells lyse without prior swelling that is a necessary step of colloid osmotic lysis. Methadone is accumulated by both cell types, and is widely distributed intracellurly in mouse leukemic cells. The maximum lytic rate is roughly proportional ...
McDonald C R - - 1978
Fulminant pneumococcal infections are rare in teen-agers with sickle cell anemia. A 16-year-old black male with sickle cell anemia was treated as an outpatient for cryptogenic pain crisis, which delayed antibiotic therapy for primary pneumococcal septicemia for seven hours. This patient did not appear ill upon initial presentation but rapidly ...
Regezi J A - - 1978
The purpose of this investigation was to specifically identify T cells, B cells, and histiocytes in the infiltrate typically seen in lichen planus. In frozen tissue sections, AET-treated sheep erythrocytes formed immunologic rosettes with the lymphocytes in the infiltrate, designating them as T cells. Rosette assays with reagent erythrocytes, IgGEA, ...
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