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Results 551 - 600 of 983
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Lekic P - - 1996
The survival rate of avulsed permanent teeth following replantation is affected primarily by the duration of the extra-alveolar period and the nature of the storage conditions. These factors are believed to strongly affect the viability of periodontal ligament (PL) cells but in vitro assays of cell viability based on vital ...
Takahashi R - - 1996
OBJECTIVE: To develop a new method of fixing and preserving cytologic specimens for immunostaining after long-term storage at room temperature. STUDY DESIGN: The method consists of three steps: fixation, freeze substitution or freeze drying, and storage. To test the method, we used the human small cell lung carcinoma cell line ...
Santos J A - - 1996
The influence of sonication power, suspension volume and cell concentration on the kinectics of cytochrome b5 and intracellular protein release by sonication of Escherichia coli TB1 cells was studied. The influence of freezing and thawing of the cell suspension was also evaluated. Freezing and thawing increased the recovery yield of ...
Seidel G E GE - - 1996
Principles and procedures for cryopreservation of equine embryos are described. Embryos less than 250 microM in diameter can be cryopreserved successfully if glycerol is used as the cryoprotectant. Cooling is takes place in such a way that most of the water leaves the cells before intracellular ice forms, and glycerol ...
Pang J M - - 1996
The present study compares the actions of the hepatotoxic agents allyl alcohol, acetaminophen, and carbon tetrachloride on energy metabolism in freshly isolated and cryopreserved rat hepatocytes. After 30 min incubation of freshly isolated hepatocytes at 37 degrees C to allow metabolic equilibration, hepatocytes were supplemented with cryoprotectants and cooled in ...
Gitlin S A - - 1996
PURPOSE: The purpose was to evaluate methods of DNA preparation in a single cell to determine the ability to amplify and correctly diagnose a targeted gene. METHODS: One- or two-cell lymphoblasts (n = 100/group), heterozygous for the normal and 4-base pair insertion on exon 11 of the beta-hexosaminidase A gene, ...
Ghiso J - - 1996
The extractive effects of n-butanol on the cell membrane have been extensively studied. Although shortterm effects of this alcohol were reported, a sustained action on tumor cells has not been described. It was previously shown that M3 mouse mammary adenocarcinoma cells pretreated with n-butanol exhibited a longer latency after subcutaneous ...
Monnet D - - 1996
Plasma apolipoprotein AI, apolipoprotein B, cholesterol and triglycerides in 114 Ivorian patients with homozygous sickle cell anaemia (HbSS) were compared to 40, age and sex matched controls with normal haemoglobin (HbAA). Except for plasma triglycerides, lipid and apolipoprotein levels were found to be lower in patients with sickle cell anaemia ...
Azadniv Mitra - - 1996
To test the hypothesis that the full extent of in vitro cell lysis due to ultrasound becomes evident with time lapse following insonation, human erythrocytes (2% hematocrit) in autologous plasma were mixed with Albunex(R), a pulse echo contrast agent, and exposed to 1-MHz, continuous-wave ultrasound (US) (5 W/cm(2) SPTA intensity) ...
Horiuchi K - - 1995
Fetal hemoglobin, Hb F, is known to be an important factor for clinical course of sickle cell disease, as it suppresses polymerization of sickle hemoglobin. To investigate the effect of Hb F on the survival of sickle reticulocytes (young red cells) in circulation, Hb F levels in individual reticulocytes and ...
Gliddon A E - - 1995
Skin biopsies have been used to establish cell cultures from ten schizophrenic individuals and ten controls. Studies of the time of outgrowth of different cell types from the tissue fragments and the subsequent growth rates and morphology showed no difference between the primary cultures from the schizophrenic patients and controls. ...
Bhunia A K - - 1995
Murine hybridoma cells, designated Ped-2E9, when stored up to 60 days at -196 degrees C or up to 48 days at -80 degrees C, gave results equivalent to those for freshly grown murine hybridoma cells in an in vitro pathogenicity assay of Listeria species. Thus, laboratories do not need to ...
Zhang Z - - 1995
Survival of media-suspended porcine erythrocytes exposed to various hydrodynamic environments was investigated with and without such shear protectant additives as bovine serum albumin, dextran and the non-ionic surfactant Pluronic F68. Erythrocytes provided a model cell population with cells of a uniform size, metabolic state and shear tolerance. Because the cells ...
Cassidy-Hanley D - - 1995
We have developed a simple, efficient procedure for the long term freezing of Tetrahymena thermophila in liquid nitrogen. This technique yields excellent recovery of viable cells with all strains tested and does not require the use of a controlled rate low temperature freezer. To optimize the freezing technique, we have ...
Chambers L A - - 1995
A closed-system filter-syringe set designed for preparation of prefiltered aliquots of packed red cells for neonatal transfusion was evaluated. In three experiments, filter-syringe sets were sterile-connected, and aliquots were prepared at six intervals during 35-day storage of CPDA-1 packed cells. Hemoglobin (HGB), supernatant potassium (K+), and free hemoglobin (fHGB) in ...
Raju G M - - 1995
For reinfusing autologous bone marrow cells after high-dose chemotherapy and/or radiotherapy it is necessary that an effective technique for their storage is available. The traditional method uses 10% dimethyl sulphoxide as cryoprotectant, a rate-controlled computerized freezer programmed to cool the cells at a constant rate of 1 degrees C per ...
Roy A - - 1995
Estimation of 3 M KCl-extracted ascites fibrosarcoma (AFS) tumor cell membrane peripheral proteins in native and frozen tumor cells showed approximately a three-, four-, and fivefold increase per 1 x 10(6) cells of single-, three-, and programmed three-cycle frozen AFS tumor cells, respectively, compared to the same number of native ...
Niwaya K - - 1995
Fibroblast viability of the allograft valve leaflet has been suggested to affect clinical durability. Warm ischemic time is thought to be one of the critical determinants of cell viability. We assessed cell viability of allograft valves by flow cytometry, using a fluorescein diacetate-propidium iodide stain to characterize the effects of ...
Mugnano J A - - 1995
Despite recent reports that antifreeze glycoproteins (AFGPs) protect mammalian cells during low-temperature preservation, T. Wang, Q. Zhu, X. Yang, J. R. Layne, and A. L. DeVries (Cryobiology 31: 185-192, 1994) reported that AFGPs failed to protect rat hearts during freezing. Rather, the presence of AFGPs exacerbated cardiac damage after freezing. ...
Reamer R - - 1995
A long-term cryopreservation study of vegetative cells of Micrococcus lutea ATCC 9341a, Micrococcus lutea ATCC 15957, and Staphylococcus epidermidis ATCC 12228 cells, used in our antibiotic bioassay procedure, was conducted. The cryoprotective abilities of 1% methylcellulose solution and a 15% glycerol solution at -14 degrees C were determined. More organisms ...
Franco R S - - 1995
A subset of sickle cells becomes K(+)-depleted and dehydrated before or soon after leaving the bone marrow. These young cells may be identified in blood as transferrin receptor-positive (TfR+) dense reticulocytes. KCl cotransport, which is normally active in young erythroid cells with a maximum at pH 6.8, is a candidate ...
Leborgne N - - 1995
The comparison of soluble sugar content in various cell lines of Eucalyptus gunnii exhibiting different freezing resistances revealed that the most resistant cell line contained the highest soluble sugar content. It was possible to increase the freezing resistance of the sensitive cell line by progressive exposure to low temperatures (acclimation). ...
Borrelli M J - - 1995
Two independent laboratories have demonstrated that suspension-grown, Chinese hamster ovary (CHO) cells can be made thermotolerant, frozen and subsequently thawed such that they still express thermotolerance. Thermotolerance was determined as the ability to protect cells against hyperthermic cell killing (colony formation assay) and the ability to reduce protein aggregation within ...
Itoh T - - 1995
To assess the role of intracellular hemoglobin concentration in the deformability of sickle (HbSS) cells after deoxygenation, rheologic coefficients (static rigidity E and dynamic rigidity eta) of density-fractionated individual sickle erythrocytes (SS cells) were determined as a function of oxygen tension (pO2) using the micropipette technique in a newly developed ...
Bryant G - - 1995
The formation of intracellular ice in biological cells during freezing is considered almost universally lethal and is the major contributor to cell damage at high cooling rates. Despite its importance, our understanding of the mechanisms of intracellular ice formation (IIF) is still incomplete. In this paper differential scanning calorimetry is ...
Callus B A - - 1995
We have used the diaminofluorene stain to detect hemoglobin production in J2E cells following erythropoietin-induced differentiation. The pseudo-peroxidase activity of hemoglobin produces a colored product, fluorene blue, which can be measured spectrophotometrically. We found that the absorbance varied with time and concentration of hemoglobin, making it unsuitable for rapid, routine ...
Hiruma H - - 1995
Sickle cell disease pathophysiology is mediated by acute and chronic impairment of cell flexibility due to the formation of intracellular sickle hemoglobin (Hb S) polymer as cells are partially deoxygenated in the microcirculation. We have recently developed a method to measure the relationship between the formation of intracellular polymerized Hb ...
Diener B - - 1994
Liver parenchymal cells (hepatocytes) of human organ donors were isolated using a two-step collagenase perfusion technique. The average viability of the freshly isolated liver parenchymal cells, as judged by trypan blue exclusion, was 82% (SD = 7%; n = 6). The inter-individual differences in the determined enzyme activities were less ...
Yu Z W - - 1994
Dimethyl sulphoxide is a water miscible solvent that has wide applications in cell biology. It acts as a cryoprotective agent in a variety of cells and tissues allowing prolonged storage at subzero temperatures. The action of dimethyl sulphoxide on the stability of the liquid matrix of cell membranes appears to ...
Rochette J - - 1994
The synthesis of fetal hemoglobin (HbF) is normally reduced to very low levels of less than 0.6% of the total hemoglobin in adults. The HbF is restricted to a sub-population of erythrocytes termed 'F-cells'; 85% of the normal adult population have 0.3% to 4.4% F-cells. The levels of HbF and ...
Ning L - - 1994
Using optical correlation interferometry, a novel method for plant sciences, we in imaged in vivo the z-direction, perpendicular to the leaf surface, through Tradescantia zebrina leaves. Non-invasively we determined number of major cell layers, followed the time sequence of decrease in cell z-axis after exposure of tissues to high salt, ...
Osnes L T - - 1994
The purpose of this study was to compare the ability of fresh and cryopreserved mononuclear cells to generate thrombin, induce fibrin formation and finally resolve the fibrin formed, when exposed to plasma. Peripheral blood mononuclear cells (PBM) from 4 donors were collected by gradient centrifugation on Lymfoprep, and cryopreserved in ...
Kirk R - - 1994
A PCR assay has been developed for the detection of Campylobacter jejuni and Camp. coli in water samples. The sample is filtered through a membrane which is subjected to sonication to release the impacted cells. After removal of the filter from the cell suspension and a freeze/thaw cell lysis step, ...
Kaeffer B - - 1994
Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of ...
Taylor S W - - 1994
Changes in vanadium coordination during cell lysis have been followed by EPR spectroscopy of the blood cells of the phleborbranch ascidians Ascidia ceratodes and PHallusia julinea. The spectra obtained for A. ceratodes whole blood samples can be mainly ascribed to aquated oxovanadium(IV) in which the signals are broadened in freshly ...
Maier-Redelsperger M - - 1994
Intracellular hemoglobin S (HbS) polymerization is most likely to be the primary determinant of the clinical and biologic manifestations of sickle cell disease (SCD). Fetal hemoglobin (HbF) does not enter the HbS polymer and its intracellular expression in sickle erythrocytes inhibits polymerization. HbF levels, high at birth but decreasing thereafter, ...
Dong A - - 1994
The reduced abnormal subunits of two M-type hemoglobins, Boston (His alpha 58-->Tyr) and Saskatoon (His beta 63-->Tyr), have been determined in the presence of normal human hemoglobin A by measurement of C-O stretch bands in infrared spectra of carbon monoxide complexes. Use of an infrared microscope coupled to a Fourier ...
Frasch S C - - 1994
The role of glycerol as a natural morphogen of myxospore formation in Myxococcus xanthus was examined. Glycerol was extracted from cells undergoing development and analyzed by gas chromatography. Glycerol is present in cells, and the intracellular level undergoes a series of transient increases during development. The data suggest a role ...
Robinson R D - - 1994
High-resolution image analysis was employed in the analysis of round (discoid) erythrocytes from hematologically normal (AA) individuals, AA individuals with nonspecific anemia, individuals with sickle cell trait (AS), individuals with SC disease (SC), and individuals with sickle cell anemia (SS). The shape feature Form Factor (4 pi Area/Perimeter2) was used ...
Wheeless L L - - 1994
Sickle cell anemia is a disease for which there is currently no effective treatment. One method of evaluating clinical status is the counting of cell types based on morphology. There is a need for a rapid, reproducible method, superior to human inspection, for classification of these cells. Quantitative digital-image analysis ...
Ishiguro H - - 1994
Experiments in which red blood cells were frozen on a directional solidification stage under a microscope show that there is a mechanical interaction between ice crystals and cells in which cells are pushed and deformed by the ice crystals. The mechanical interaction occurs during freezing of cells in physiological saline ...
Zhang S Q - - 1994
Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 microM, significantly inhibited guard-cell PEPC activity of closed stomata but not ...
Franco R S - - 1994
A subset of sickle cells have an increased density at the reticulocyte stage of development, indicating that they are either abnormally dense upon release from the bone marrow or become dense quickly in the circulation. These cells are of interest because they most likely have severely disrupted cation regulation and ...
Milburn P T - - 1994
Recombinant cells of Saccharomyces cerevisiae, expressing virus-like particles (Ty-VLPs), can be readily disrupted in a high pressure homogenizer and show identical disruption kinetics to the untransformed host strain. When the cells are freeze/thawed before disruption, they become about four times more resistant to homogenization. This effect increases with the number ...
Fibach E - - 1994
In order to clarify the mechanism of the effect of erythropoietin (Epo) on the fetal haemoglobin (HbF) phenotype of peripheral erythrocytes, we studied the dose-response effect of Epo on HbF production by erythroid precursors derived from the peripheral blood of normal adult individuals and grown in a two-phase liquid culture ...
Dworkin M - - 1994
The role of glycerol as a natural morphogen of myxospore formation in Myxococcus xanthus was examined. Glycerol was extracted from cells undergoing development and analyzed by gas chromatography. Glycerol is present in cells, and the intracellular level undergoes a series of transient increases during development. The data suggest a role ...
Balgir R S - - 1994
Sickle cell hemoglobinopathy has emerged as a major public health problem in the tropical countries of the world including India. This paper deals with the age at menarche and first conception of women in relation to sickle cell genotypes. The mean age at menarche was slightly higher in sickle cell ...
Ochkur S I - - 1994
The objective of this study was to estimate the effect of cryopreservation on the main pathways of energetic metabolism and motility of fowl spermatozoa. Sperm diluted 1:5 with the cryoprotective medium containing ethylene glycol (1.4 M final concentration) was frozen at the rate of 2-3 degrees C/min to -25 degrees ...
Lipp G - - 1994
In this study the interaction of human erythrocytes in suspension with a planar ice-liquid interface is investigated. Due to a repulsive van der Waals force repelling the cells from the crystal and an attractive force resulting from the viscous drag of melt flow around the cell a velocity was determined ...
Pakkanen R - - 1994
A mixture containing an ultrafiltrate fraction (UF) of bovine colostrum (6.7%), adult bovine serum (BS) (1%), and human holo-transferrin (hTF) (5 mg/liter) was developed for cultivation of Chinese hamster ovary cells (CHO-K1) and African green monkey kidney cells (Vero). The growth-supporting activity of the mixture (UF/BS/hTF) was comparable to that ...
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