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Cittadini A - - 1981
The Ca2+ ionophore A23187 increases intracellular calcium content in normal thymic cells, while it is without effect on the corresponding neoplastic cell (Ascites thymoma) and on Ehrlich ascites tumour cells. The A23187-induced total cell calcium increase in normal thymocytes takes place both in control and energy-depleted cells, while it is ...
Nelson W J - - 1981
A simple method of analytical subcellular fractionation, combined with a sensitive computational method for data analysis and presentation, has been used to reinvestigate the distribution and relative amounts of several enzymes in the cytoplasmic and plasma membranes of two different cell types: one is a neoplastic, transformed cell type (Ehrlich ...
Austin S A - - 1981
1. Addition of haem to cell-free extracts from Ehrlich ascites tumour cells stimulates protein synthesis only in extracts from cells previously incubated in nutritionally complete conditions. Extracts from amino-acid-deprived cells do not respond to haem. The stimulation of protein synthesis in fed cell extracts is due to increased initiation on ...
Kusyk C J - - 1981
Karyotype analyses were done on cells from the primary and metastatic sites, as well as on the ascitic fluid, from 1 patient with serous carcinoma of the ovary. An increase in the proportion of near-tetraploid cells occurred in the following order: cells from the primary site less than cells from ...
Braun R - - 1981
The cytostatic and mutagenic compound N'-methyl-N'-cyano-(p-chloro)-benzaldehyde hydrazone (CyB4) has been found to be a strong SH-blocking agent since it reacts with the thiol groups of glutathione and of the cell membrane of Ehrlich ascites carcinoma cells (EAC). Furthermore, it decreases the intracellular, non-proteinogenic SH(NPSH)-level of tumor cells. CyB4 is not ...
Apelgot S - - 1981
Three to 20 hr after an i.p. injection of 64Cu (half-life, 12.8 hr) into mice bearing Krebs ascites cells, a high amount of the radioisotope was recovered in the ascites cells themselves. In the control group, the radioisotope was mainly present in the liver. Similar amounts of 64Cu were recovered ...
Bozzi A - - 1981
Addition of 0.5mm-daunomycin, a quinone anti-cancer drug, causes severe inhibition of respiration in Ehrlich ascites cells, whereas Yoshida ascites cells were almost as resistant as rat hepatocytes. An inverse relationship appears to exist in the two types of tumour cells (which are both catalase-deficient) between the extent of cellular damage ...
Krieglstein J - - 1981
In earlier work it has been shown that mitochondrially bound brain hexokinase is solubilized by anesthetics. This effect was reevaluated using cultured cells. For the present experiments Ehrlich ascites, Harding-Passey melanoma, C-1300-neuroblastoma and C-6-glioma cells were used. The great portion of hexokinase activity bound to the mitochondria of these cells ...
Furusawa E - - 1981
A narcissus alkaloid, pretazettine hydrochloride (PTZ), has been shown to be active against Ehrlich ascites carcinoma. The Ehrlich cells are more sensitive to PTZ in DBA/2 mice than in Swiss mice. The therapeutic activity of the individual standard drugs in ABC (adriamycin, BCNU, and cyclophosphamide) regimen against Ehrlich ascites carcinoma ...
Nöhammer G - - 1981
Mercurochrom (2,7-dibromo-4-(hydroxymercuri)-fluoresceine-disodium salt) reacts histochemically not only with protein-SH-groups, but is also bound unspecifically to cellular proteins. The amount of the unspecifically staining approximately equals the specific SH-staining. Two methods are described to remove the unspecifically bound Mercurochrom, without influencing the specific reaction with the protein thiols. The first applies ...
Krishnamurti C - - 1980
The interactions of ethylenediaminetetraacetic acid (EDTA) and 1,10-phenanthroline with Ehrlich cells have been studied. Both compounds inhibit proliferation of cells in culture. After long-term incubation of cells with these metal-chelating agents under conditions in which cell viability is not impaired but proliferation is retarded, the rate of DNA synthesis of ...
Olivotto M - - 1980
A method is described for determining the duration of cell cycle phases traversed by cells responding to release from proliferation restraint. Experiments have been performed with arrested Yoshida ascites hepatoma cells allowed to re-enter the growing stage after transfer of cells from the late stage of ascites into an in ...
Woodard L F - - 1980
Whole cells of Brucella abortus strain 45/20 were combined with trehalose dimycolate or muramyl dipeptide. These preparations were tested in guinea pigs for immunogenic properties. Both trehalose dimycolate and muramyl dipeptide were found to be effective adjuvants to whole cells when combined in oil emulsions. Although saline suspensions of whole ...
Korohoda W - - 1980
It has been observed that a dolichol, alpha dihydro-undecaprenol, induces phenotypic changes in Ehrlich ascites tumour [EAT] cells. In the presence of this dolichol in the medium. EAT cells attach to glass and spread on it but grow in an overlapping pattern. This spreading takes place after a lag of ...
Koch J - - 1980
The properties of Ehrlich ascites tumour cells exposed in vivo to cadmium were investigated as a function of the zinc status of the host animals. Tumour-cell growth was inhibited by cadmium in both zinc-sufficient and zinc-deficient animals. However, cells in zinc-sufficient tumours accumulate much less cadmium than those in deficient ...
Al-Hashimi A H - - 1980
Ehrlich ascites tumour cells (E.A.T.) in suspension were exposed to ultrasound of frequency 1x5 MHZ, intensities 1x4, 2x8 W/cm2 for 60 min at 37 degrees C. The suspensions of E.A.T. cells were contained in Perspex vessels with Melinex windows, situated in the near field of a piezoceramic transducer. Subsequently the ...
Kawakami H - - 1980
Proteoglycan (heparan sulfate-protein conjugate) was solubilized with 8 M urea from rat liver plasma membranes after enzymic (RNAase, neuraminidase) treatments and extensively purified by chromatography and gel filtration. The final products gave an average ratio of hexuronate to protein (weight) of approx. 1.5, contained hexosamine equimolar to hexuronate and were ...
Matsumoto I - - 1980
The complex carbohydrates at the cell surfaces of two TA3, murine mammary carcinoma ascites sublines (the strain-specific, TA3-St subline and the nonstrain-specific. TA3-Ha line) were compared by binding studies with 125I-labelled concanavalin A (con A), Ricinis communis agglutinin (RCA), and eel-serum agglutinin (ESA). The TA3-Ha cell bound equal amounts of ...
Woodman P W - - 1980
Isoelectric focusing and studies with 1-(2'-deoxy-beta-D-glucopyranosyl)thymine (GPT), a specific inhibitor of uridine phosphorylase activity, were used to determine the substrate specificities of mammalian pyrimidine nucleoside phosphorylases and their cleavage of 5-fluoro-2'-deoxyuridine (FdUrd). Isoelectric focusing profiles for the cytosol fractions from Ehrlich ascites cells and from Novikoff hepatoma cells each consisted ...
Suganuma T - - 1980
Trypsin treatment, which is frequently used in the field of immunocytochemistry to faciliate conjugated antibody penetration through the cell membrane, was applied for the immunoradiographic demonstration of alpha-fetoprotein in ascites hepatoma cells using 125I-labeled anti rat alpha-fetoprotein antibody. No significant number of silver grains were detectable in the group without ...
Sawada H - - 1979
Analysis of the liberation degrees of intracellular materials from Ehrlich ascites tumor cells by treatment of Triton X-100 was performed. The liberation process has two stages. In the first stage, cytoplasmic inorganic phosphorus were liberated at a concentration of about 0.01% of the detergent, and in the second stage macromolecules ...
Taki T - - 1979
The effect of butyrate on glycolipid metabolism and morphological differentiation in the cell culture system of rat ascites hepatomas, AH 7974 of island-forming type and AH 7974F of free type, was studied. Both cell lines adhered to the substratum in the presence of 1 mM butyrate. In the case of ...
Cullen B M - - 1979
Cells of the mouse Ehrlich ascites carcinoma grown in vitro became contaminated with an arginine-splitting mycoplasma. The slopes of the radiation dose-survival curves of the contaminated cells, assayed by colony-forming ability, were extremely variable; eventually it became impossible to grow colonies at all. Experiments on the feeder cell requirement showed ...
Bukrinskaya A G - - 1979
The properties of fowl plague virus (Influenzavirus A) nucleocapsids isolated from the cytoplasm of infected Ehrlich ascites carcinoma cells and chick embryo cells were compared. Nucleocapsids isolated from both systems possessed similar polypeptides (P and NP) but differed in their biophysical characteristics. Nucleocapsids from ascites cells sedimented in velocity sucrose ...
Corchs J L - - 1979
Potassium influx, intracellular potassium and sodium content and cellular volume were determined in vitro in Ehrlich ascites cells in the presence of up to 0.8 mM bilirubin in the incubation medium. Bilirubin uptake into cells as a function of bilirubin concentration in the incubation medium increased linearly with a molar ...
Codington J F - - 1979
A new non-strain-specific ascites subline of the TA3 mammary adenocarcinoma TA3-MM, which arose in vivo from the strain-specific TA3-St subline during an acute respiratory illness of the syngeneic mouse strain A/HeHa hosts, possessed at its surface a glycoprotein not found on the parent TA3-St cell. This glycoprotein, termed TA3-MM epiglycanin, ...
Manchester K L - - 1979
The decrease induced by appropriate initiation factors in [Mg] required for optimal polyphenylalanine synthesis primed by poly(U) has been used as an assay to test for the presence of these factors in cytosol of rat liver and muscle and of ascites cells. By calculation and from changes in [Mg] optima ...
Eardley D D - - 1979
Lyl T cells are able to induce B cells to make antibody and also to induce a resting Lyl23 T cell set to exert potent feedback suppression. Which of these two pathways Lyl T cells take can be influenced by the mode of immunization. In particular, in vitro immunized Lyl ...
Solaiman D - - 1979
Ehrlich ascites tumor cells accumulate cadmium against a concentration gradient in a bisphasic uptake process. There is little efflux of the metal from preloaded cells into a cadmium-free medium. Incorporation of 3H-thymidine into DNA is markedly inhibited by cadmium ion at 5-100 ng atoms of Cd/mg of cell protein, but ...
Hayashi M - - 1979
Studies were made on the effect of cancer chemotherapeutic drugs on in vitro differentiation of a clone (R4) of mouse myeloid leukemic cells (MI) that is resistant to inducers. Treatment of the cells with 50% ascitic fluid (an inducer) plus 0.25 microgram/ml of adriamycin or 0.3 microgram/ml of daunomycin induced ...
Sato N L - - 1979
The tRNA nucleotidyltransferase activity (3H-CMP incorporation into 3'-terminus of tRNApC) in cytoplasmic fractions of various types of cells such as Ehrlich ascites tumor cells, mouse liver and spleen cells, rat spleen, lymph node, and macrophages cells was found to be dependent on the concentrations of nucleoside 5'-triphosphates (ATP, GTP, UTP, ...
Trump B F - - 1979
Exposure of Ehrlich ascites tumor cells to anoxia resulted in rapid and characteristic conformational changes of cell surface topography. Combined scanning and transmission E/M studies revealed rapid alterations including simplification of the cell surface configuration with disappearance of microvilli which were replaced with formation of blebs and recesses at the ...
Langen P - - 1979
A series of halogeno (chloro or fluoro) analogues of phospholipid precursors has been checked for their cytostatic activity both in vitro and in vivo. The compounds included monoacyl-, diacyl-, monoalkyldeoxyhalogenoglycerols and other acylhalogenoalkanols. Analogues of monoacylglycerols or monoalkylglycerols were found to have a strong inhibitory activity on the proliferation of ...
Tropé C - - 1979
Cell suspensions from nine human ovarian primary cancers, their metastases and ascitic cells were treated in vitro with amethopterin and melphalan. Effects were measured by incorporation of H3-TdR or H3-UdR into the cells. There was significant heterogeneity of cytostatic effects on cells from the three sources in a given patient. ...
Wolpert W - - 1978
Immunoprecipitation of polyribosomes from Streptomyces hydrogenans synthesizing 20beta-hydroxysteroid dehydrogenase was performed by immunoelectrophoresis. Only polyribosomes from cells induced by 11beta, 21-dihydroxy-4, 11(20)-pregnadien-3-one in vivo were precipitated by immunoglobulins specific for 20beta-hydroxysteroid dehydrogenase. No unspecific precipitations with polysomes from control cells cultivated in the absence of steroids were observed. Therefore, 11beta, ...
Simonsen L O - - 1978
A regulatory function of the cell membrane in controlling the cytoplasmic level of Pi has been proposed, and in Ehrlich ascites tumor cells an active influx of primary phosphate has been reported in the literature. In the present study, Ehrlich cells were incubated at 1.5--50 mM extracellular Pi at pH ...
Knöchel W - - 1978
Cell-free synthesis of globin chains in the presence of globin mRNA in an Ehrlich ascites-cell-free system is further stimulated by addition of 18- and 28-S rRNA but not of 4-S tRNA and 5-S rRNA. This stimulation can not be observed in the wheat germ cell-free system. When 125I-labelled globin mRNA ...
Agren G - - 1978
A transfer of (32P)-labeled phosphoryl groups has been demonstrated to occur between prelabeled Ehrlich cells (donor cells) and glioma cells (acceptor cells). The Ehrlich cells were generally prelabeled in vivo by an intraabdominal injection of (32P)-orthophosphate 24 hours prior to scarifice of the tumor-bearing mice. In some experiments two injections ...
Hirabayashi Y - - 1978
A comparative study of the glycolipids was performed on two cell lines of rat ascites hepatomas, island-forming and free cell types, and several marked differences were found as follows: 1. Hexose content in glycolipids derived from AH 7974F (free cell type) was about 3.6-fold as much as that from AH ...
Codington J F - - 1978
In a series of six TA3-HA/A.CA hybrid cell lines formed by the fusion of the TA3-HA mammary carcinoma of a strain A mouse with a normal embryonic fibroblast of an A.CA mouse and then converted to the ascites form in parental strain A, the capacity to grow in foreign strains ...
Hanaoka Y - - 1978
Two tumour-cell-aggregation factors, derived from rat ascites hepatoma cells, had different antigenicity; one was not absorbed by immunoadsorbent chromatography with anti-rat serum antibody and the other was. Their activities were both lost by digestion with trypsin, but remained unchanged by oxidation with periodate, suggesting the role of the protein portions ...
CieĊ›lak J - - 1978
The experiments were carried out in order to find factors which could induce attachment and spreading of Ehrlich ascites tumour (EAT) cells on solid substrata. In normal culture media, serum-free as well as serum-containing, these cells did not spread and very weakly attached onto glass. It was found that after ...
Maslow D E - - 1978
The presence of small numbers of tumour cells inhibits the aggregation of embryonic chicken neural retina cells grown in gyratory shaker culture. The aggregation of neural retina cells was also inhibited by ascites cell medium. We investigated whether the inhibitory effect of the tumour cells on aggregate size is effected ...
Lepoint A - - 1978
Fractions of mouse Ehrlich ascites tumor cell populations with a high percentage of cell either in early or in late interphase were separated by centrifugation on ficoll gradients. Nucleoli were studied by light or electron microscopy in these cell subpopulations. It was shown that, in these cells, the number of ...
Nakada H - - 1978
Two types of ascites hepatoma cells, AH 66 and AH 130 FN, were treated with trypsin to observe the release of complex carbohydrates constituting the plasma membranes. From AH 66 cells, mucopolysaccharide (heparan sulfate) was preferentially released. From AH 130 FN cells, N-glycosidic glycopeptides were preferentially released whereas no mucopolysaccharide ...
Poydock M E - - 1978
The selective action of two fractions of PCO (a yeast extract) on normal and malignant cells was demonstrated. In vivo, the mitotic activity of malignant cells was inhibited by the methanol-insoluble fraction of PCO, whereas the methanol-soluble fraction caused no inhibition. The in vitro studies, however, showed inhibition of mitoses ...
Drobnica L - - 1978
1,4-Dithiaanthraquinone-2,3-dicarbonitrile (DTA) has been found to exert a considerable cytostatic effect especially on some of the investigated types of eukaryotic cells, concretely on the HeLa cells, moulds, yeasts, protozoa and algae. In cells of the Ehrlich ascites carcinoma (EAC) DTA after a short exposition causes a parallel inhibition of incorporation ...
Koshimura S - - 1977
Cytoplasmic membrane of Group A streptococcus has been obtained by treatment of the cells with a phage-associated lytic enzyme to dissolve the streptococcal cell wall, followed by shocking osmotically. The protoplast membrane fraction (PMF) remained as a distinct homogeneous structure in the electron micrograph and analysis showed a low rhamnose ...
Shah K J - - 1977
Fifteen 7-substituted 4-hydroxyquinoline-3-carboxylic acids have been designed to minimize covariance between the physicochemical substituent parameters: pi, MR, and sigmap. The molecules have been synthesized and evaluated for their ability to inhibit the respiration of Ehrlich ascites cells as a whole cell model and for their ability to inhibit malate dehydrogenase ...
Kallio A - - 1977
1. Ehrlich ascites-carcinoma cells contained relatively high concentrations of spermidine and spermine, but the putrescine content of the washed cells was less than 10% of that of higher polyamines. 2. Ascites-tumour cells likewise exhibited high activities of L-ornithine decarboxylase (EC 4.1.1.17), S-adenosyl-L-methionine decarboxylase (EC 4.1.1.50), spermidine synthase (EC 2.5.1.16) and ...
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