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Results 201 - 250 of 362
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Prokazova N V - - 1984
The glycolipid composition of Ehrlich ascites carcinoma cells was found to depend strongly on the cell density of the suspension. The general trend observed upon dilution of the cell suspension was a reduction of the less complex gangliosides GM3 and GM2 with concomitant increase of the more complex gangliosides, especially ...
Shaposhnikova G I - - 1984
The ganglioside composition of mouse ascites hepatoma ( MAH ) cells, the ascites fluid and cell-conditioned media were determined and found to be qualitatively identical, but quantitatively different. The ganglioside content of the ascites fluid and the medium conditioned by MAH -cells at the native cell concentration (10(8) cells/ml) comprised ...
Betz M - - 1984
Incubation of Ehrlich ascites cells with normal or C1q or C2 deficient human sera results in killing of the cells. Killing occurred also in the absence of free Ca++, which supported by the fact that factor B and C3 were cleaved, leads to the conclusion that the alternative pathway of ...
Racker E - - 1984
Placental polypeptides present in crude preparations of transforming growth factors stimulate glycolysis when added to quiescent 3T3 cells, normal rat kidney, and chick embryo fibroblasts. The stimulation was apparent over a time period of at least 90 min and was seen at glucose concentrations ranging from 1 to 30 mM. ...
Kallio A - - 1984
Treatment of Ehrlich-ascites-carcinoma-bearing mice with methylglyoxal bis(guanylhydrazone) alone or in combination with 2-difluoromethylornithine greatly enhanced the transfer of intragastrically administered radioactive putrescine and cadaverine into the carcinoma cells. Difluoromethylornithine alone did not have any effect on the accumulation of intestine-derived diamines in the tumour cells. The frequently reported restoration of ...
Murakami H - - 1984
Hybrid cell lines which secreted antibodies to liver-specific membrane lipoprotein (LSP) were obtained by immunizing SMA and BALB/c mice with human LSP and fusing their splenocytes with the myeloma cell line P3-NSI/1-Ag4-1. The secretion of antibody to LSP (anti-LSP) was monitored by binding to a human hepatocellular carcinoma cell line, ...
Balzarini J - - 1984
Two strategies have been pursued to monitor the inhibition of thymidylate (dTMP) synthase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) by thymidine (dThd) analogs in intact murine leukemia L1210 cells. The first method was based on the determination of tritium release from 2'-deoxy[5-3H]uridine [( 5-3H]dUrd) or 2'-deoxy[5-3H]cytidine [( 5-3H]dCyd); the second method was ...
Stoehr M - - 1984
The cytokinetic response of Ehrlich ascites tumor (EAT) cells in vivo upon chronic treatment at low dosage levels with cytarabine (1-beta-D-arabinofuranosylcytosine, ara-c) bleomycin (BLM) and peplomycin (PEP) was estimated. Bivariate DNA histograms allow the simultaneous evaluation of the cell cycle status of living and killed cells. It could be confirmed ...
Moller P C - - 1984
Although adenyl cyclase (AC) has been reported for many mammalian cell types its presence in transformed cells is controversial since AC activity is present in some tumors but absent in others. Thus Chang hepatoma ascites cells were investigated to determine if AC could be localized cytochemically in this cell type. ...
Papageorgiou A - - 1984
The effect of a homo-aza-steroidal ester (ASE) on the incorporation of radioactive precursor to DNA of Ehrlich ascites tumor (EAT) cells has been investigated. We found that treatment of cells with 80 micrograms/ml of ASE for 2 hours causes an inhibition of the incorporation of 3H-thymidine to DNA by 71%. ...
v F Kieler J - - 1983
Two types of 'spontaneous' malignant alteration in vitro of ST/a mouse lung fibroblasts (ST-L) have been observed. In contrast to cells which retained their fibroblastic appearance (R- ST-L cells), cells showing morphological signs of transformation (R+ ST-L cells) developed strong isoimmunizing properties. Both types of cells expressed MuLV antigens which ...
Savage K E - - 1983
We determined the effect tunicamycin (TM), an inhibitor of protein glycosylation, had on cells in vitro that were derived from solid and ascites variants of a chemically induced rat hepatoma. Using flow microfluorometry (FMF), labelling index (LI), and population-doubling time assays, we monitored the progression of cells through the cell ...
Olah I - - 1983
We have observed the rapid development (4 to 7 hr after injection) of an ascitic condition in the hepatoperitoneal sac after the i.v. injection of large amounts of carbon. The amount of ascitic fluid varied from .5 to 12 ml/chicken. The protein content of the fluid was identical with that ...
Peterson M S - - 1983
dUDP-GlcNAc, the 2'-deoxyribosyl analogue of UDP-GlcNAc, has been identified in human lymphoid cells treated with the dihydrofolate reductase inhibitor, methotrexate. It was shown previously that elevation of dUTP accompanies the gross expansion in intracellular deoxyuridylate pools that results from the methotrexate-induced block in thymidylate synthetase activity (1). dUDP-GlcNAc presumably is ...
Minoda M - - 1983
This study was undertaken to clarify the function of thymic reticuloepithelial cells (TRC) in the New Zealand black (NZB) mouse as a model animal of human autoimmune disease. Attention was focused on whether or not any defect in the TRC function participating in the maturation process from immature T cells ...
Laiho K U - - 1983
The possible protective effects of 30 and 180 mM mannitol added to the extracellular medium were studied in Ehrlich ascites tumor cells (EATC) injured by the nonpenetrating mercurial p-chloromercuribenzenesulfonic acid (PCMBS) or by anoxia. Protection was provided with 180 mM mannitol as observed by vital dye uptake, cell volume determinations, ...
Kroll W - - 1983
In order to elucidate the significance of oxidative ATP production for the proliferation of Ehrlich ascites tumor cells, cell cycle progression, energy metabolism and macromolecular synthesis in the presence of oligomycin were studied. In the presence of the inhibitor (20 micrograms/ml), lactate production and glucose uptake of the cells increased ...
Renau-Piqueras J - - 1983
Stereologic ultrastructural methods were applied to several cell types involved in the production of monoclonal antibodies against the mitochondrial enzyme glutamic dehydrogenase (GDH). These cell types included SP2/O murine cells, in vitro cultured hybrid cells producing monoclonal antibodies, and the same hybrid cells maintained as solid and ascites tumors (TH ...
McTavish C F - - 1983
The turnover of vimentin and vimentin-derived peptides has been examined in logarithmically growing Ehrlich ascites tumour cells. Cells were pulse-labelled with [35S]methionine for 30 min and then chased for up to 60 h. It was found that the specific radioactivity of the main isoelectric variant of vimentin decreased to half ...
van den Eijnden D H - - 1983
Novikoff ascites tumor cell homogenate was found to catalyze the transfer of [14C]N-acetylglucosamine from UDP-[14C]GlcNAc to asialo-alpha 1-acid glycoprotein. Mucins appeared to be poor acceptors. Methylation and hydrolysis of the product formed in an incubation with UDP-GlcNAc and asialo-alpha 1-acid [3H]glycoprotein yielded 2,4,6-trimethyl [3H]galactose and 2,3,4-trimethyl [3H]galactose, indicating that N-acetylglucosaminyl ...
Jacobsen H - - 1983
Interferon treatment of mouse JLS-V9R cells resulted in a 10- to 20-fold increase in the levels of the 2-5A (ppp(A2'p)nA)-dependent RNase. The nuclease was monitored in cell extracts by covalent and noncovalent binding of 32P-labeled 2-5A derivatives to the nuclease and by the appearance of 2-5A-mediated ribosomal RNA cleavage products. ...
Witte P L - - 1983
Intrasplenic inoculation of small numbers (10(4)) of antibody-producing hybridoma cells into unprimed BALB/c mice resulted in high-titered ascites. By intrasplenic injection, the low number of cells needed to produce ascites was 2-3 logs fewer than that required by ip inoculation. Hybridoma cells suspended in the ascitic fluid could be reestablished ...
Nissen E - - 1983
Single cells from a patient (B.E., 65 years) have been isolated by collagenase treatment and cultivated in vitro. The human tumour showed predominantly simple undifferentiated and also tubular structures. The 5th in vitro passage of these cells were characterized by growth curves, DNA-distribution patterns and chromosome numbers. Cells of the ...
Böhmer F D - - 1983
Binding of 125I-insulin to Ehrlich ascites carcinoma cells revealed the presence of high affinity binding sites characterized by a dissociation constant of 6.1 . 10(-9) M and a number of sites per cell of 2.6 . 10(3). These values are in line with respective data for other cell types reported ...
Lund-Andersen C - - 1983
The influence of anthracyclines on the uptake of actinomycin D in Ehrlich ascites tumour cells was studied in vitro. The anthracycline daunorubicin significantly increased the [3H]actinomycin D uptake both in whole tumour cells and in isolated nuclei of tumour cells. On the other hand, actinomycin D increased daunorubicin uptake only ...
Kono K - - 1983
Studies have been undertaken to clarify the localizing properties of radioactively labeled antibody to AFP using ascitic hepatoma cells which produce AFP. In the in vitro studies, radioantibodies to AFP were incorporated into the AFP-producing cells, even though excess antigen existed in the medium and the antibodies remained on the ...
Lehmann W - - 1983
The following aspects of action of a chalone-like factor for the Ehrlich ascites mammary carcinoma and of the granulocytic chalone are discussed: 1) Dependence on the state of proliferation. Rapidly growing cells respond poorly or not at all. 2) Antagonism with growth factors. The factor for the Ehrlich ascites mammary ...
Bogajewski J - - 1983
A method causing an increase of the number of anaphases of animal cells in vivo, suitable for mutagenicity testing, is presented. The Ehrlich ascites cells transplanted in mice were used as test cells. The increase of the number of anaphases (8-23/1000) was obtained by: 1. transplantation of the ascites tumour ...
Skog S - - 1983
The cell-cycle related energy metabolism after roentgen irradiation of in vivo growing Ehrlich ascites tumour cells was investigated in cell fractions obtained by elutriator centrifuging. The oxygen consumption and the lactate and pyruvate production, measured in vitro after 4.5, 5 and 9 Gy up to 24 h, were undisturbed, while ...
Parsons D F - - 1982
Interaction of Krebs-2 and Ehrlich tetraploid cells with NYLR/Nya mouse peritoneum mesothelium and penetration of basal lamina and elastic reticulum were studied. Invasion of abdominal viscera was rare. Invading cells had a shrunken nucleus and cytoplasm like the "dark cells" of hyperplastic epithelia. High-voltage electron microscope stereoscopy showed that invasive ...
Traub P - - 1982
Polyribosomes were isolated from Mg2-stabilized, Triton X-100-resistant residual cell structures and from a postmitochondrial supernatant of hypotonically swollen and homogenized Ehrlich ascites tumor cells and analysed for their vimentin contents by polyacrylamide gradient slab gel electrophoresis. Whereas polyribosomes from detergent-resistant cell residues were accompanied by a small amount of vimentin ...
Higuchi Y - - 1982
A protein fraction (60-F), obtained from cell-free extract of living hemolytic streptococcus, Su-strain, by 50-60% saturation with ammonium sulfate, inhibited the de novo synthesis of nucleic acids and proteins in Ehrlich ascites carcinoma (EAC) cells. 60-F released RNA but not DNA from EAC cells. This cytotoxic or cytolytic effect of ...
Nato F - - 1982
Cell growth of tumour ascites cells was inhibited by concanavalin A, phytohaemagglutinin and Ricinus lectin at 2-100 micrograms/ml. As expected, the Ricinus lectin inhibited the protein synthesis estimated by leucine incorporation and decreased thymidine incorporation, whereas concanavalin A and phytohaemagglutinin stimulate the uptake and the incorporation of both leucine and ...
Ginsberg B H - - 1982
We have altered the phospholipid composition of the plasma membranes of Ehrlich ascites cells, grown in mice and studied the effects on the properties of the insulin receptor of this cell. The insulin receptor of the Ehrlich cell demonstrated all of the binding characteristics of mammalian insulin receptors: specificity for ...
Merz R - - 1982
Centrifugal elutriation was applied to separate into the cell compartments asynchronous Ehrlich ascites cells grown under different culture conditions. The cytokinetic properties of the recultivated fractions were studied by flow cytometry. The present experiments prove that G1-cohorts grown 12 h under exclusion of oxygen accumulate in the late G1-compartment. The ...
Smetana K - - 1982
To provide more information on the number of silver-stained granules (SSGs) of active mitotic nucleolus organizer regions (NORs) as well as interphase nucleoli, rat Yoshida ascitic sarcoma and Zajdela ascitic hepatoma cells were studied by means of standardized one-step and two-step silver-staining procedures. The number of SSGs of mitotic active ...
Nakazawa I - - 1982
Male Donryu rats weighing from 100 to 120 g were transplanted with AH100B, AH66F or Yoshida sarcoma (YS) cells intraperitoneally. After collecting ascites, while not bloody, the cancer cells were washed three times with Dulbecco's phosphate buffered saline (PBS) without Ca++ and Mg++ (pH 7.2). Then, each sediment was suspended ...
Miller S C - - 1982
The loss of strain specificity in the TA3-St mammary carcinoma ascites cell during passage in ascites form in diseased syngeneic strain A mice was confirmed by the observation that TA3-MM/2 ascites cells were capable of progressive growth in 7 foreign mouse strains. Support for the hypothesis that allotransplantability in the ...
Saffer J D - - 1982
The phosphorylation of the high mobility group (HMG) proteins has been investigated in mouse Ehrlich ascites, L1210 and P388 leukemia cells, human colon carcinoma cells (HT-29), and Chinese hamster ovary cells. HMG 14 and 17, but not HMB 1 and 2, were phosphorylated in the nuclei of all cell lines ...
Wheeler G P - - 1982
Ethyl 5-amino-1,2-dihydro-3-[N-methylanilino)methyl]pyrido[3,4-b]pyrazin-7-ylcarbamate (NSC 181928) is reported to be active against several experimental neoplasms. The experimental data obtained in the present study indicate that it causes the accumulation of cells at mitosis with both cultured cells and ascites cancer cells in vivo. This effect was observed with L1210, P388, colon cancer ...
Iapalucci-Espinoza S - - 1982
The nucleoside analogues fluorouridine and fluorodeoxyuridine (both at 100 muM) and 8-azaguanine (at 500 muM) inhibit both rRNA transcription and processing in Ehrlich ascites cells. In BHK21 cells fluorodeoxyuridine has no effect on either rRNA maturation or transcription, whereas toyocamycin (at 2 microM) inhibits both processes in BHK21 cells and ...
Kang Y H - - 1982
The correlation between the cytochemistry (glycoprotein, glycogen, glucose-6-phosphatase, catalase, alkaline phosphatase) and the growth rate of the fast-growing Morris hepatoma 3924A and the slow-growing Morris hepatoma 9618A was studied by utracytochemical techniques. By the chromic acid-phosphotungstic acid technique, acid glycoprotein is stained in glycocalyx, Golgi saccules and vesicles, and secretory ...
Likovský Z - - 1982
Yoshida ascitic sarcoma and Zajdela ascitic hepatoma were investigated in Norway rats to provide more information on active nucleolus organizer regions (NORs) represented by silver stained granules (SSGs). Diploid Yoshida sarcoma cells were characterized by the presence of 6 (3 doublets) of SSGs in the metaphase and 3 + 3 ...
Morinaga T - - 1982
Zajdela rat ascites hepatoma cells have been reported to be 'nonsecretors' of alpha-fetoprotein (AFP). Because of the potential of such cells in the study of defective secretory mechanisms of AFP and other serum proteins, we concluded a detailed investigation of the production of AFP in Zajdela cells growing in vivo. ...
Schneider E L - - 1981
To examine the effect of an aging environment on sister chromatid exchange (SCE) induction, Ehrlich ascites tumor (EAT) cells were introduced into young and old C57BL/6J mice. Background SCE levels were not significantly different in either EAT cell or normal bone marrow cell populations between young and old animals. Despite ...
Steven F S - - 1981
Ehrlich ascites cells have been shown to possess a protease with beta-naphthylamidase activity located on the surface of these cells. This enzyme is protected from the inhibitory action of protein inhibitors of trypsin (EC 3.4.21.4) in free solution, but is inhibited by high concentrations of active site-directed inhibitors of trypsin. ...
Reimann J - - 1981
The intravenous transfer of polyclonally activated lymphoblasts elicited a proliferative response of host-derived Lyt1 T cells in the spleens of syngeneic recipient mice. These Lyt1 T cells (responding in vivo to a syngeneic lymphoblast graft) stimulated proliferation of resting, syngeneic splenic Lyt1 T cells, derived from uninjected mice, in a ...
De la Fuente M - - 1981
The effect of a new synthetic C-nucleoside (2-beta-D-lyxopyranosyl-6,6-dimethyl-4,5,6,7-tetrahydroindol-4-one) on cell differentiation and growth was studied through three kinds of experiments: a) Appearance rate of peripheral blood cells in AKR mice, b) Growth capacity of Ehrlich ascite tumor cells and c) Reappearance rate of membrane receptors on T-lymphocytes which from E-rosette ...
Cittadini A - - 1981
The Ca2+ ionophore A23187 increases intracellular calcium content in normal thymic cells, while it is without effect on the corresponding neoplastic cell (Ascites thymoma) and on Ehrlich ascites tumour cells. The A23187-induced total cell calcium increase in normal thymocytes takes place both in control and energy-depleted cells, while it is ...
Nelson W J - - 1981
A simple method of analytical subcellular fractionation, combined with a sensitive computational method for data analysis and presentation, has been used to reinvestigate the distribution and relative amounts of several enzymes in the cytoplasmic and plasma membranes of two different cell types: one is a neoplastic, transformed cell type (Ehrlich ...
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