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Miko M - - 1994
The main purpose of the present investigation was to study the effect of the homologous series of 4-alkylmorpholine N-oxides on ATP-producing processes in Ehrlich ascites and L1210 murine leukaemia cells. The effects on aerobic glucose consumption, lactic acid formation, content of total (T-SH) and non-protein thiol groups (NP-SH), endogenous and ...
Zhang Y - - 1994
Using 3-(4,5-dimethythiazole)-2,5-diphenyltetrazolium bromide (MTT) method, the effect of probimane (Pro) on doxorubicin (Dox) cytotoxicity was studied. Pro 0.313, 0.625, and 1.25 micrograms.ml-1 potentiated cytotoxicity of Dox in Ehrlich ascites carcinoma (EAC) cells. Incubation of EAC cells with Dox 10 micrograms.ml-1 and Pro 116.5, 233, and 466 micrograms.ml-1 resulted in an ...
Ng T B - - 1994
1. alpha-Momorcharin, a glycoprotein isolated from seeds of the bitter gourd, Momordica charantia inhibited incorporation of [3H]thymidine, [3H]leucine and [3H]uridine into P388 (mouse monocyte-macrophage), J774 (Balb/c macrophage), JAR (human placental choriocarcinoma) and sarcoma S180 cell lines. 2. The most potent inhibitory effect was exerted on P388 cells and the smallest ...
Menninger J R - - 1993
At nonpermissive temperatures the peptidyl-tRNA hydrolase of pth(Ts) bacterial mutants is inactivated, and cells accumulate peptidyl-tRNA and die. Doses of erythromycin, lincomycin, or clindamycin that inhibited the growth of antibiotic-hypersensitive DB-11 pth+ cells accelerated the killing of DB-11 pth(Ts) cells at nonpermissive temperatures. Erythromycin and lincomycin also stimulated the accumulation ...
Ohshima S - - 1993
A cell surface-associated adhesive factor (AF) separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) has been highly purified by chromatography. AF is assumed to mediate the cell-cell adhesion essential to island formation of the hepatoma cells. A substance, immunologically cross-reactive with AF, is present in ...
Miko M - - 1993
The aim of this study was to study the effect of the homologous series of 1-alkylpyrrolidine N-oxides on ATP-producing processes in Ehrlich ascites and L1210 murine leukemia cells. 1-Decylpiperidine N-oxide (deP-NO), one of the active compounds, significantly stimulated the course of aerobic glycolysis of Ehrlich cells. Derivatives with longer side-chains ...
Ogura H - - 1993
The organization of RSV genes present in the mouse ascites sarcoma cells was analysed. The transformed cells kept in vivo and in vitro for a long time continued to contain intact RSV genome and the cellular integration site was unchanged. RSV was recovered by transfection of chick embryo cells with ...
Mostafapour M K - - 1993
Ehrlich ascites tumor cells (EAT cells) are routinely grown in the peritoneal cavity of mice. These cells, EAT-wt, grow in suspension and exhibit a high level of alpha-2,3-O-linked sialyltransferase activity with benzyl-T-antigen (Gal beta 1,3Ga1NAc-alpha-O-CH2C6H5) as acceptor. These cells also contain a very low level of alpha-2,6-O-linked and alpha-2,6-N-linked sialyltransferase ...
Thiry M - - 1993
The fine spatial distribution of rRNA within Ehrlich tumour cells has been investigated during the cell cycle by in situ hybridization with a biotinylated probe. In interphase cells, rRNA was essentially detected in the ribosome-rich cytoplasmic regions and in the three main nucleolar components. In mitotic cells, besides the presence, ...
Gu Y C - - 1993
Effects of methanol on colony-formation of human hepatoma cells were investigated. Among five human hepatoma cell lines (Hep G2, HLE, HuH-6, HuH-7, and PLC/PRF/5), only HLE cells showed enhanced colony formation due to methanol. The effective concentrations of methanol were around 1%. The enhancement occurred in a greater degree when ...
Bílá V - - 1993
The acyclic nucleotide analogues, HPMPC and PMEA, differ in their in vitro effect on the genetic material of eukaryotic cells. While HPMPC exerts a cytostatic effect on eukaryotic cells in vitro, PMEA has a genotoxic activity. These results correspond with the mode of embryotoxic action of these compounds: HPMPC exhibits ...
Digoutte J P - - 1992
Successive experiments led us to use two cellular systems, MOS61 (Aedes pseudoscutellaris cells) and Vero cells, among the continuous cell lines recommended by the WHO Collaborating Center for systematic research and isolation of arboviruses. Virus detection in cell cultures is carried out with 7 mixtures containing 10 hyperimmune ascitic fluids ...
Liu Y - - 1992
The effects of morphine administration were evaluated upon the polymorphonuclear (PMN) cell activities of rhesus macaques. Sixteen animals were used in the study. Seven macaques were treated with saline and nine animals were administered morphine in the form of morphine sulfate, following a control period of saline injections. The dosage ...
Scaloni A - - 1992
During protein biosynthesis, processing of the N terminus of many proteins may occur through acetylation and deacetylation. The enzyme acylpeptide hydrolase is likely involved in deacetylation of nascent peptide chains or of bioactive peptides. The related enzyme, acylase, hydrolyzes the acetyl amino acid product of the acylpeptide hydrolase reaction to ...
Lea M A - - 1992
1. The specificity of the action of orotate on hepatoma cells was investigated. 2. Orotic acid and its methyl ester had similar inhibitory effects on the incorporation of [3H]thymidine into DNA of hepatoma cells. 3. In contrast to previous studies in vivo, incubation of rat kidney cells with orotate caused ...
Xhonneux B - - 1992
Itraconazole, a systemically active antifungal, was tested for its effects on microscopically assessed phagocytosis and killing of Candida glabrata 233 in vitro. Yeast cells were exposed to itraconazole in culture and guinea-pig peritoneal polymorphonuclear leucocytes were exposed to the drug injected intraperitoneally in vivo. At a concentration of 10(-7) M ...
Miko M - - 1992
The main purpose of the present investigation was to study the effect of cloturin on aerobic glycolysis, endogenous and exogenous respiration and the level of ATP in both Ehrlich ascites carcinoma (EAC) and P388 murine leukaemia cells incubated in vitro. Also its effect on the level of total (T-SH) and ...
Hirte H W - - 1992
Rapid isolation of tumor cells growing in clumps from the ascitic fluid of patients with ovarian carcinoma was achieved by harvesting cells from ascitic fluid and subsequently passing them over a 30-microns nylon mesh filter. Single cells and small cell aggregates passed through the mesh, and large cell clumps that ...
Siems W G - - 1992
A balance of ATP-consuming processes in human erythroleukemia (K 562) cells by use of the decreased 14CO2 formation from [1-14C]-glutamate following inhibition of energy-requiring processes is presented. This method was tested on Ehrlich mouse ascites tumour cells and was used in suspensions of K 562 cells with a low cell ...
Miko M - - 1992
The main purpose of the present investigation was to study the effect of 9-hydroxybenfluron (HBF) on aerobic glucose consumption, lactic acid formation, content of total (T-SH) and non-protein thiol groups (NP-SH), endogenous respiration and levels of ATP in both Ehrlich ascites and P388 murine leukemia cells. The lowest concentrations of ...
Rotmensch J - - 1992
Cell flow cytometry offers the opportunity to analyze cytopathological samples with regards to DNA content and proliferative activity. To investigate whether this modality can quantitate certain aspects of ovarian carcinoma by analyzing ascites, 43 samples from patients with advanced papillary serous adenocarcinoma of the ovary were studied. In 28 samples ...
Gabai V L - - 1992
ATP depletion in EL-4 ascites tumour cells rapidly induced the changes in cell morphology (blebbing), cytoskeletal protein assembly and finally resulted in cell death. After 1 hr of incubation with 2 microM rotenone (inhibitor of respiration) in glucose-free medium, when ATP level was 4% of the initial level, there were ...
Miko M - - 1991
This paper presents data on the effects of benfluron and its two metabolites DBF and NOBF on both endogenous and exogenous, respiration in the presence of succinate as substrate, of both P388 murine leukemia and Ehrlich ascites carcinoma cells. The most efficient inhibitors of endogenous and exogenous respiration were benfluron ...
McSheehy P M - - 1991
Quantitative analysis of extracts from two Ehrlich ascites tumour cell lines (Lettre cells) by 19F NMR in vitro demonstrated that one Lettre cell line (designated LLM) metabolized 30-50% less 5-fluorouracil to 5-fluoronucleotides when compared to the other cell line (designated LHM). HPLC analysis of these cellular extracts showed a significant ...
Ohira Y - - 1991
The effect of implantation of Ehrlich ascites tumor (EAT) cells on creatine distribution was investigated. It was also studied how depletion of creatine by feeding creatine-analogue beta-guanidinopropionic acid (beta-GPA) affects the growth of EAT cells in mice. Enhanced mobilization of creatine from host tissues to EAT cells against a greater ...
Wolf F I - - 1991
We have previously observed that extracellular Mg2+ influences the phosphofructokinase (PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed ...
Lazebnik Y A - - 1991
Using flow cytometry we found that proliferation of Ehrlich ascites carcinoma (EAC) cells has been reversibly arrested in the second half of the G2 period at the plateau phase of tumor growth in vivo. The ratio of G2/G1 cells increased from 0.3 at 6 days post tumor inoculation to 2.5 ...
Pryme I F - - 1991
The activity of aminoacyl-tRNA synthetase phosphatase as well as the activities of aminoacyl-tRNA synthetases in Krebs II ascites cells and MPC-11 cells have been investigated. The activity of the phosphatase was greater in the tumor cells than in normal tissues. The aminoacyl-tRNA synthetase activities were 100-300 times higher than the ...
Matsui-Yuasa I - - 1991
The activity of ornithine decarboxylase (ODC) was increased in Ehrlich ascites tumor cells by a change of the medium. This increase in the activity was inhibited by the addition of LiCl to the medium. Na+ and Mg2+ did not affect the enzyme activity. The inhibition of the enzyme activity with ...
Ray M - - 1991
The effect of methylglyoxal (MG), ascorbic acid and lactaldehyde has been tested on the in vitro respiration of Ehrlich ascites carcinoma (EAC) cells and several normal and malignant human tissues. Methylglyoxal inhibited the respiration of each type of malignant cell and tissue tested, but it had practically no inhibitory effect ...
Prasad S B - - 1991
Scanning electron microscopic studies revealed that ascites Dalton's lymphoma cells are distributed singly (25-30%) or in groups of 2-3 cells (55-60%) and 5 or more cells (10-15%) connected together. The percentage of single cells and groups of 2-3 or more cells changes with tumor growth. The number of single cells ...
Kovacević Z - - 1991
A comparative study revealed that Ehrlich ascites carcinoma (EAC) cells use glutamine plus inosine for regeneration of adenylates via the purine nucleotide cycle, whereas AS 30D hepatoma cells use adenosine instead. This observation can be correlated with the very low production of aspartate from glutamine in hepatoma cells. Although glucose ...
Hirte H - - 1991
The effect of cell-free ascitic fluid from patients with epithelial ovarian carcinoma on the generation of lymphokine-activated killer cells (LAK) was compared to the activity generated in control medium containing 10% fetal bovine serum, using Daudi target cells. Samples of ascitic fluid from nine different patients tested inhibited LAK generation. ...
Nicotina P A - - 1991
Some cytochemical and immunocytochemical investigations were carried out on actively growing Yoshida ascites sarcoma cells. These cells displayed an intense granular alpha-naphthylacetate esterase (ANAE) staining while the alpha-naphthylbutyrate esterase (ANBE) reaction was in part fluoride-sensitive and marked particularly in the large-size malignant cells. Acid phosphatase as well as peroxidase activities ...
Kalraiya R D - - 1990
A not well-appreciated but clinically important aspect of malignant tumours is their effects on distantly located host cells. The effects, termed paraneoplastic syndromes, also pose an intriguing mechanistic problem: how do malignant cells influence properties of host cells not in contact with them? Erythrocytes from the circulation of rats bearing ...
Obara Y - - 1990
A new hypodiploid stemline of the long-lasting ascites sarcoma, Hirosaki sarcoma (HS), was established by means of single-cell transplantation, and its G-, C- and NOR-banding patterns were compared with those of the predecessor stemline, with special notice taken of the structural alteration of marker chromosomes. Their chromosomal relationship was well ...
Wójciak E - - 1990
Adhesiveness of Ehrlich ascites tumour (EAT) cells to glass, to mouse peritoneal membrane, living and aldehyde-fixed mouse embryo fibroblasts and chick embryo fibroblasts, myoblasts and myotubes was investigated. The ascitic EAT cells (and leukaemia L1210 cells) did not adhere to glass and peritoneum but readily adhered to embryo fibroblasts, myoblasts ...
He Q M - - 1990
Three isoenzymes of thymidine kinase (TK) identified on Ehrlich ascites tumour corresponding to pl values of 5.3, and 6.9 and 8.3 were studied with respect to their kinetic properties. The isoenzymes were separated by thin-layer isoelectric focusing and measured in the presence of thymidine (TdR) as substrate, adenosinetriphosphate (ATP) as ...
Bharathan S - - 1990
The MAT-B1 and MAT-C1 ascites sublines of the 13762 rat mammary adenocarcinoma contain a dominant cell surface "complex" consisting of two glycoproteins: ascites sialoglycoprotein (ASGP)-1, a Mr 600,000-700,000 peanut agglutinin-binding sialomucin, and ASGP-2, a Mr 120,000 concancavalin A-binding glycoprotein (Sherblom et al., J. Biol. Chem., 255: 783-790, 1980; Sherblom and ...
Pinchuk V G - - 1990
Comparison of the morphometric patterns obtained by electron microscopy with data from the biochemical analysis of metabolism in Ehrlich ascites tumour cells and P-388 leukemia cells, in each phase of the cell cycle, shows a distinct correlation between structure and function which is demonstrated by the fact that stage duration ...
Prosser E - - 1990
The effects of haematoporphyrin (Hp), haematoporphyrin derivative (HpD), coumarin, 7-hydroxycoumarin, 4-hydroxycoumarin, amino-methyl-coumarin acetic acid and acridine orange, with and without photoradiation, on Landschutz ascites tumour (LAT) cells were examined. Hp, HpD and acridine orange showed a significant effect on LAT cell viability following photoradiation. However, coumarin or its derivatives had ...
Kuttan G - - 1990
The effect of Iscador, a commercial preparation made from Viscum album was studied on several cell lines using in vitro tissue culture as well as tumor-bearing animals. Iscador was found to be cytotoxic to animal tumor cells such as Dalton's lymphoma ascites cells (DLA cells) and Ehrlich ascites cells in ...
Punnonen E L - - 1990
The microtubule inhibitor vinblastine causes accumulation of autophagic vacuoles in many cell types. In hepatocytes, many of the accumulated vacuoles are nascent, which has been interpreted to suggest that vinblastine acts by inhibiting the fusion of hydrolase-containing lysosomes with early autophagic vacuoles. However, our previous results suggested that, in Ehrlich ...
Adamietz I A - - 1990
Changes of the cell surface proteolytic activity during the cell cycle in vitro were reported. Using an easy assay, with casein as a substrate, the proteolytic activity on the surface of Ehrlich ascites tumor (EAT) cells grown in vivo was determined. The cleavage of casein incubated with EAT cells increased ...
Ofosu G A - - 1990
Ultrastructural studies of the effects of the chemotherapeutic agent platinum-thymine, on the morphology of sarcoma-180 ascites cells were studied to elucidate the cancer cells immediate response to therapy and the possible mode of cancer cell regression. Sarcoma-180 ascites cells treated with platinum-thymine in vitro at concentrations of 60 micrograms/ml at ...
Wasserman L - - 1989
The effect of sodium butyrate was examined on the growth and phenotypic expression of a cell line derived from the ascitic fluid of an untreated patient with ovarian carcinoma. The chemical inducer of differentiation, sodium butyrate, markedly enhances the activity of the membrane-bound glycoprotein enzymes, alkaline phosphatase and gamma-glutamyl transpeptidase. ...
Ritter C - - 1989
Lettre-Ehrlich cells were loaded with sufficient HN2 to produce about a 98% cell kill. Postincubation of the HN2-loaded cells in PBS resulted in the loss of about 40% of their HN2 without changing the cytolytic effect, supporting the proposal that only bound drug was effective. Postincubation of the HN2-loaded cells ...
Ritter C - - 1989
Steady-state uptake of choline by Lettre-Ehrlich tumor cells in vitro, resulting in cell-to-medium ratios of 10 or more, is significantly increased by 0.2-1.0 mM Ca++ as well as by dipalmitoyl phosphatidyl choline multilamellar liposomes + Ca++. The increases occur in spite of a decrease in carrier affinity, as indicated by ...
Nakazawa I - - 1989
In order to establish metastatic lesions, 2.5 x 10(6) AH100B cells were injected into the left carotid artery of male Donryu rats. Each metastatic nodule in the liver or kidney, 1 mm or less in diameter, thus obtained was then injected into the peritoneal cavity in which these metastatic cells ...
Ohigashi H - - 1989
To study the tissue preference of invasion, we developed an assay system for the invasion of endothelial cells as a modification of the previously established assay of tumor cell invasion of mesothelial cells. Rat ascites hepatoma cells (AH 130) that had been seeded on a monolayer of cultured endothelial cells ...
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