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Malicka-Blaszkiewicz Maria M Department of Cell Pathology, Faculty of Biotechnology, University of Wrocław and Nonpublic Medical College in Wrocław, Wrocław, - - 2014
Ovocystatin is a chicken egg white protein, generally known for its inhibitory activity against cysteine proteases. However, biological activity of ovocystatin does not seem to be well recognized in respect to other possible cellular effects. Our attention has been focused on ovocystatin cytotoxic effects in relation to its influence on ...
Wang Hongtao H Department of Pathology, School of Medicine, Southeast University, Nanjing 210009, - - 2014
Arsenic trioxide (As2O3) has shown therapeutic effects in some leukemias and solid cancers. However, the molecular mechanisms of its anticancer efficacy have not been clearly elucidated, particularly in solid cancers. Our previous data showed that As2O3 induced apoptosis of human papillomavirus (HPV) 16 DNA-immortalized human cervical epithelial cells and cervical ...
Kennedy Edward M EM Departments of Molecular Genetics & - - 2014
High-risk human papillomaviruses (HPVs), including HPV-16 and HPV-18, are the causative agents of cervical carcinomas as well as being linked to several other tumors of the anogenital and oropharyngeal regions. The majority of HPV-induced tumors contain integrated copies of the normally episomal HPV genome that invariably retain intact forms of ...
Wei Lanlan L Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang ChinaDepartment of Molecular Genetics and Microbiology, The University of New Mexico School of Medicine, Albuquerque, NM - - 2014
High-risk human papillomavirus (HR-HPV) infections are necessary but insufficient agents of cervical and other epithelial cancers. Epidemiological studies support a causal, but ill-defined relationship between tobacco smoking and cervical malignancies. In this study, we used mainstream tobacco smoke condensate (MSTS-C) treatments of cervical cell lines that maintain either episomal or ...
Kováčik Andrej A Skin Barrier Research Group, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Heyrovského 1203, 50005 Hradec Králové (Czech - - 2014
Sphingolipids are crucial for the life of the cell. In land-dwelling mammals, they are equally important outside the cell-in the extracellular space of the skin barrier-because they prevent loss of water. Although a large body of research has elucidated many of the functions of sphingolipids, their extensive structural diversity remains ...
Rios-Yuil Jose M JM Departments of Dermatology and Pathology of the Panamanian Social Security (Caja de Seguro Social) and San Fernando Hospital (Clinica Hospital San Fernando), Panama City, Panama; Postgraduate studies and Research Section, Superior School of Medicine, Instituto Politecnico Nacional, Mexico City, Mexico, - - 2014
Background: Information is scarce about the presence of molecular alterations related to human papillomavirus (HPV) infection in squamous cell carcinomas of the genital skin and about the effect of this infection in the number of Langerhans cells present in these tumors. Aims: To determine the presence of HPV in genital ...
Cohen Cynthia C Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, - - 2014
With the increased number of requests for high-risk human papilloma virus in situ hybridization (HPV ISH), not only for uterine cervical squamous cell carcinoma (SCC) but also for biopsies and resections of oropharyngeal SCC, and fine needle aspiration cell blocks of metastatic SCC in cervical lymph nodes, we optimized an ...
Wang Yongfang Y Department of Pharmacology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, - - 2014
Condylomata acuminata (CA), induced by low-risk human papillomaviruses (HPVs), is one of the most common sexually transmitted diseases. The increasing incidence and the high recurrence rate of CA have significantly contributed to public health problems around the world. Because HPVs cannot be cultured in vitro for a long time, there ...
Malecka Kimberly A - - 2014
Cervical cancer is the sixth most common cancer in women worldwide and the leading cause of women's death in developing countries. Nearly all cervical cancers are associated with infection of the human papillomavirus (HPV). This sexually transmitted pathogen disrupts the cell cycle via two oncoproteins: E6 and E7. Cells respond ...
Rouiller-Fabre Virginie V UMR 967, Inserm, CEA, DSV/iRCM/SCSR/LDG, unit of stem cells and radiation, laboratory of development of the gonads, university Paris Diderot, Sorbonne Paris Cité, route du panorama, BP 6, 92265 Fontenay-aux-Roses, - - 2014
The modern societies are exposing us to a huge variety of potentially harmful pollutants. Among these endocrine disruptors (EDs) have been especially scrutinized as several were proven to display reprotoxic effects in rodent models. In the context of high and growing concerns about the reprotoxicity of EDs, it is crucial ...
Merne Marina M Department of Oral Pathology, Institute of Dentistry, Faculty of Medicine and MediCity Research Laboratory, University of Turku, Turku, - - 2014
The scope of this work was to study synergism between human papillomavirus (HPV) infection and tobacco in vitro, both known to be independent risk factors for oral cancer. HPV-positive and HPV-negative oral keratinocytes and oral HPV-negative fibroblasts were exposed to smokeless tobacco extract (STE) prepared from the Scandinavian (STE1) and ...
Arenz Andrea A Department of Radiotherapy and Radiooncology, BMFZ-Biomedical Research Center, Philipps-University, Hans-Meerwein-Straße 2, 35043, Marburg, Germany, - - 2014
Human Papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) respond favourably to radiotherapy as compared to HPV-unrelated HNSCC. We investigated DNA damage response in HPV-positive and HPV-negative HNSCC cell lines aiming to identify mechanisms, which illustrate reasons for the increased sensitivity of HPV-positive cancers of the oropharynx. Radiation response ...
Kranjec Christian C International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34149 Trieste, - - 2014
The cancer-causing high-risk HPV E6 oncoproteins target a number of cellular proteins that contain PDZ domains. However the role of many of these interactions in either the HPV life-cycle or in HPV-induced malignancy remains to be defined. Previous studies had shown that MAGI-1 was one of the most strongly bound ...
Sangthong Supranee S Program of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, - - 2014
A new series of anthracene-9, 10-dione derivatives have been synthesized to increase cytotoxic activity against human papillomavirus (HPV) positive cancer cell line, CaSki. The highest cytotoxicity was achieved by 4-(benzylamino)-9,10-dioxo-4a,9,9a,10-tetrahydroanthracen-1-yl 4-ethylbenzenesulfonate (5) with the inhibitory concentration 50 (IC50) of 0.3 μM which is 20 times lower than that of cisplatin (CDDP; IC50 = 8.0 μM). ...
Kotnik Halavaty Katarina K Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, 303 E. Chicago Avenue, Chicago, IL 60611, - - 2014
Human papillomaviruses (HPV) infect stratified epithelia and link their life cycles to epithelial differentiation. The HPV E5 protein plays a role in the productive phase of the HPV life cycle but its mechanism of action is still unclear. We identify a new binding partner of E5, A4, using a membrane-associated ...
Mori Seiichiro - - 2014
Co-infection of multiple genotypes of human papillomavirus (HPV) is commonly observed among women with abnormal cervical cytology, but how different HPVs interact with each other in the same cell is not clearly understood. A previous study using cultured keratinocytes revealed that genome replication of one HPV type is inhibited by ...
Valentino L A LA Departments of Pediatrics, Internal Medicine, Immunology/Microbiology and Biochemistry, Section of Pediatric Hematology/Oncology, RUSH Hemophilia and Thrombophilia Center, RUSH University Medical Center, Chicago, IL, - - 2014
The development of inhibitors and the need for frequent venous access for FVIII injection are major challenges in current haemophilia treatment. Presently available recombinant FVIII (rFVIII) products produced in hamster cell lines are associated with inhibitor formation in up to 32% of previously untreated patients. The new human cell line-derived ...
Pfitzner Claudia C Department for Gynaecology and Obstetrics, Jena University Hospital, Friedrich Schiller University, - - 2014
The detection of circulating tumour cells (CTC) in cancer patients may be useful for therapy monitoring and prediction of relapse. A sensitive assay based on HPV-oncogene transcripts which are highly specific for cervical cancer cells was established. The Digital-Direct-RT-PCR (DD-RT-PCR) combines Ficoll-separation, ThinPrep-fixation and one-step RT-PCR in a low-throughput digital-PCR ...
Cheng Yong - - 2014
Purpose: GRIM-19 has been shown to be down-regulated in cervical cancers. This study investigated the expression of GRIM-19 in cervical intra-epithelial neoplasias and its association with high-risk human papillomavirus (HR-HPV) infection. Methods: The expression of GRIM-19 was assessed in cervical exfoliated cells and cervical intra-epithelial neoplasia tissues by immunohistochemistry, and ...
Horton Jaime S JS Laboratory of Experimental Medicine; John A. Burns School of Medicine; Honolulu, HI USA ; Department of Cell and Molecular Biology; John A. Burns School of Medicine; Honolulu, HI - - 2014
Epidermodysplasia verruciformis (EV) is a rare genodermatosis characterized by increased sensitivity to infection by the β-subtype of human papillomaviruses (β-HPVs), causing persistent, tinea versicolor-like dermal lesions. In a majority of affected individuals, these macular lesions progress to invasive cutaneous squamous cell carcinoma (CSCC) in sun-exposed areas. While mutations in transmembrane ...
Rodes Laetitia - - 2013
Abstract This study evaluates alginate-poly-l-lysine-alginate Bifidobacterium longum subsp. infantis ATCC 15697-loaded microcapsules to enrich the human gut microbiota. The cell survival of alginate-poly-l-lysine-alginate microencapsulated B. infantis ATCC 15697 in gastric acid, bile, and through human gastrointestinal transit was investigated, as well as the formulation's effect on the gut microbiota. Results ...
Washiya Kiyotada - - 2013
Objective: To increase the accuracy of the diagnosis of atypical squamous cells of undetermined significance (ASC-US), ASC-US were divided into high-risk human papillomavirus (HPV HR+) and non-high-risk HPV (HPV HR-) cases to analyze the significance of binucleated cells with compression. Study Design: ThinPrep specimens of ASC-US were examined. This study ...
Recho P P LMS, CNRS-UMR 7649, Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, - - 2013
We propose a mechanism for the initiation of cell motility that is based on myosin-induced contraction and does not require actin polymerization. The translocation of a cell is induced by symmetry breaking of the motor-driven flow, and the ensuing asymmetry gives rise to a steady motion of the center of ...
Sørensen Brita Singers - - 2013
HPV associated Head and Neck Squamous Cell Carcinoma (HNSCC) represents a distinct subgroup of HNSCC characterized by a favorable prognosis and a distinct molecular biology. Previous data from the randomized DAHANCA 5 trial indicated that HPV positive tumors did not benefit from hypoxic modifications by Nimorazole during radiotherapy, whereas a ...
Busch Chia-Jung - - 2013
Despite the comparably high cure rates observed for HPV-positive HNSCC, there is still a great need for specific tumor radiosensitization due to the often severe side effects resulting from intense radiochemotherapy. We recently demonstrated that HPV-positive HNSCC cell lines are characterized by a defect in DNA double-strand break repair associated ...
O'Neill John D JD Department of Biomedical Engineering, Columbia University, New York, New York 10032, - - 2013
The only definitive treatment for end-stage organ failure is orthotopic transplantation. Lung extracellular matrix (LECM) holds great potential as a scaffold for lung tissue engineering because it retains the complex architecture, biomechanics, and topologic specificity of the lung. Decellularization of human lungs rejected from transplantation could provide "ideal" biologic scaffolds ...
Kimple Randall J RJ Department of Human Oncology, University of Wisconsin Comprehensive Cancer Center, 3107 WIMR, 1111 Highland Avenue, Madison, WI 53705, USA. - - 2013
Patients with human papillomavirus (HPV+)-associated head and neck cancer (HNC) show significantly improved survival outcome compared with those with HPV-negative (HPV-) tumors. Published data examining this difference offers conflicting results to date. We systematically investigated the radiation sensitivity of all available validated HPV+ HNC cell lines and a series of ...
Jia Wenzhao - - 2013
No sweat, no gain: Flexible biofuel cells functionalized with lactate oxidase (LOx) and platinum as anode and cathode materials harvested biochemical energy from human perspiration. Substantial power was generated from human sweat in real-life scenarios.
Cuschieri Kate - - 2013
The relative and analytical sensitivity of the APTIMA HPV test (AHPV, broad-spectrum, target amplification) and the PreTect HPV-Proofer (type-specific, target amplification) for the detection of HPV mRNA in various cell lines was compared. Equivalent relative sensitivity for the HPV 16-containing cell lines (2.5 cells/ml with both CaSki and SiHa) was ...
Spoden Gilles G Department of Medical Microbiology and Hygiene, University Medical Centre of the Johannes Gutenberg University, Mainz, - - 2013
Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and ...
Brown Kristy J - - 2013
Proteomic analysis of human body fluids is highly challenging, therefore many researchers are redirecting efforts towards secretome profiling. The goal is to define potential biomarkers and therapeutic targets in the secretome that can be traced back in accessible human body fluids. However, currently there is a lack of secretome profiles ...
Rieckmann Thorsten - - 2013
BACKGROUND AND PURPOSE: When treated by radiotherapy, patients with squamous cell carcinomas of the head and neck (HNSCC) positive for HPV and p16(INK4a) possess a clearly favorable prognosis as compared to those with HPV-negative HNSCC. The aim of this work was to study whether the better outcomes might be caused ...
Tang Alice L AL Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan 48109, - - 2013
To determine if the behavior of cancer stem cells (CSCs) is affected by human papillomavirus (HPV) status. An in vitro and in vivo analysis of HPV and CSCs. University laboratory. We isolated CSCs from HPV-positive and HPV-negative cell lines. Two HPV-negative cell lines underwent lentiviral transduction of E6/E7. Chemoresistence was ...
Nagel Remco R Department of Otolaryngology/Head-Neck Surgery, VU Medical Centre, PO Box 7057, 1007 MB Amsterdam, The - - 2013
Infection with the human papillomavirus (HPV) is an important risk factor for development of head and neck squamous cell carcinoma (HNSCC). Strikingly, HPV-positive HNSCCs have a more favorable prognosis than their HPV-negative counterparts. The current study was designed to explain this favorable prognosis of HPV-positive HNSCC. This was performed by ...
Cun Biyun - - 2013
Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. However, the molecular development of UM is not fully understood and current therapeutic modalities result in poor outcomes. Increasingly, data have shown that human papillomaviruses (HPVs) contribute to the development of cervical cancer and other malignancies, and the ...
DeCaprio James A JA Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215, USA. - - 2013
During the past 6 years, focused virus hunting has led to the discovery of nine new human polyomaviruses, including Merkel cell polyomavirus, which has been linked to Merkel cell carcinoma, a lethal skin cell cancer. The discovery of so many new and highly divergent human polyomaviruses raises key questions regarding ...
Li Changyou C Department of Medicine, University of Pittsburgh and the University of Pittsburgh Cancer Institute, Pittsburgh, PA, - - 2013
Human papilloma virus (HPV) infection represents an emerging risk factor in head and neck squamous cell carcinoma (HNSCC). In contrast to HPV-negative HNSCC, most cases of HPV-positive HNSCC encode wild-type p53, although the p53 protein in these cells is rapidly degraded via HPV E6-mediated ubiquitination and subsequent proteasomal degradation. This ...
Pongcharoen Sutatip - - 2013
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the ...
Laird Diana J DJ Department of Ob/Gyn, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Science, UCSF, 35 Medical Center Way, San Francisco, California 94143, USA. - - 2013
Primordial germ cell (PGC) development in the human fetus remains relatively uncharted. A new study suggests that epigenetic reprogramming and sex differentiation in human PGCs occur asynchronously over an extended time period. This finding raises questions and implications for in vitro PGC differentiation.
Androphy Elliot J - - 2012
Comment on: Mirza S, et al. Cell Cycle 2012; 11:4266-74.
Barthel Erik R - - 2012
Aim: Loss of colon reservoir function after colectomy can adversely affect patient outcomes. In previous work, human fetal intestinal cells developed epithelium without mesenchyme following implantation in mice. However, for humans, postnatal tissue would be the preferred donor source. We generated tissue-engineered colon (TEC) from postnatal human organoid units. Materials ...
Jackson Robert - - 2012
Human papillomavirus (HPV) type 16 E6 is a viral oncoprotein essential for host cell transformation. Due to its role in HPV-induced cancers of the genital, head and neck epithelia, reliable protein-level determination of E6 expression would be an invaluable diagnostic tool. Immunocytochemical detection and subcellular localization of HPV16 E6 has ...
Kannicht Christoph - - 2012
INTRODUCTION: Host cell lines used for recombinant protein expression differ in their ability to perform post-translational modifications (PTMs). The currently available recombinant human FVIII (rhFVIII) products are produced in mammalian, non-human cell lines. For rhFVIII, glycosylation and sulfation are vital for functionality and von Willebrand factor (VWF)-binding affinity. Here we ...
Conesa-Zamora Pablo - - 2012
Cervical cancer (CC) is caused by a persistent infection by certain human papillomavirus (HPV) genotypes. Although Papanicolaou (Pap) Test is considered the most cost-effective test for reducing CC mortality a considerable number of high-grade precursor lesions of CC could pass unnoticed with the Pap. The addition of high-risk human papillomavirus ...
Li Yun-Zhi - - 2012
A new phenylpropanoid, (+)-methyl 3-acetoxy-3-(7-methoxy-1,3-benzodioxol-5-yl) propanoate (1), was obtained from the 95% EtOH extract of entire plant of Peperomia tetraphylla. The structure was elucidated based on the spectral analysis (IR, 1-D and 2-D NMR and HRESIMS). The absolute configuration of 1 was determined as (3R) by referring to analogous compound's ...
Zeller Perrine - - 2012
Microcystins (MCs) are cyclic hepatotoxins produced by various species of cyanobacteria. Their structure includes two variable amino acids (AA) giving rise to more than 90 MC variants, however most of the studies to date have focused on the most toxic variant: microcystin LR (MC-LR). Ingestion is the major route of ...
Tong Nannan - - 2012
The clinical use of doxorubicin (DOX), a potent antineoplastic agent, is limited by its serious side-effects, which include acute and chronic cumulative dose-related cardiotoxicity. Berberine (BER), a botanical alkaloid, has been reported to possess cardioprotective and antitumor effects. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-tetrazolium bromide (MTT) assay was used to detect the cell viability ...
Fraga Carlos Alberto de Carvalho - - 2012
This study investigated the immunodetection of CD57 inflammatory cells in patients with head and neck squamous cell carcinoma (HNSCC) and its association with clinicopathological parameters and overall survival. Data collected from the morphological analysis and immunohistochemical reaction testing of archived HNSCC specimens (n=70) were statistically analyzed by bivariate and multivariate ...
Warnecke Athanasia - - 2012
The treatment of choice for profound sensorineural hearing loss (SNHL) is direct electrical stimulation of spiral ganglion cells (SGC) via a cochlear implant (CI). The number and excitability of SGC seem to be critical for the success that can be achieved via CI treatment. However, SNHL is associated with degeneration ...
Ferranti F - - 2012
The glial cell line-derived neurotrophic factor (GDNF) has multiple functions that promote cell survival, proliferation and migration in different cell types. The experimental over-expression of GDNF in mouse testis leads to infertility and promotes seminomatous germ cell tumours in older animals, which suggests that deregulation of the GDNF pathway may ...
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