Search Results
Results 401 - 450 of 1692
< 4 5 6 7 8 9 10 11 12 13 14 >
Hsiung Lo-Chang - - 2008
Uniform patterning of cells is highly desirable for most cellular studies involving cell-cell interactions but is often difficult in an in vitro environment. This paper presents the development of a collagen-coated planar interdigitated ring electrode (PIRE) array utilizing positive dielectrophoresis to pattern cells uniformly. Key features of the PIRE design ...
Heiskanen Arto R - - 2008
An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance ( R ct) to the redox process ...
Lyon Alexander R AR National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, - - 2008
Cardiac cell therapy is an expanding scientific field which is yielding new insights into the pathogenesis of cardiac disease and offers new therapeutic strategies. Inherent to both these areas of research are the electrical properties of individual cells, the electrical interplay between cardiomyocytes, and their roles in arrhythmogenesis. This review ...
S. Lerson, J.L. Lilien, ...
The performance of a 5 kW Solid Oxide Fuel Cell (SOFC) will be studied and described in this paper. This will help to significantly reduce residential Green House Gas (GHG) emission to about 20%, when compared with the actual emissions, based on the high-performance boiler and electricity produced by high-efficiency ...
Suzuki Masato - - 2008
In this paper, a novel method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent microelectrode subunits was fabricated with indium-tin-oxide (ITO) and used as a template to form cellular ...
Shi L - - 2008
Despite growing interest in the electro-bioremediation of contaminated soil it is still largely unknown to which degree weak electric fields influence the fate of contaminant-degrading microorganisms in the sub-surface. Here we evaluate the factors influencing the electrokinetic transport and deposition of fluorene-degrading Sphingomonas sp. LB126 in a laboratory model aquifer ...
Kirschbaum Michael - - 2008
The gentle and careful in vitro processing of live cells is essential in order to make them available to future therapeutic applications. We present a protocol for the activation of single-T cells based on the contact formation with individual anti-CD3/anti-CD28 presenting microbeads in a lab-on-chip environment. The chips consist of ...
Kang Hyen-Wook - - 2008
For investigating the effects of chemical stimulation to cultured cells, we have developed a quartz crystal sensor system with a micro charge-coupled device (CCD) camera that enables microphotograph imaging simultaneously with quartz crystal measurement. Human hepatoma cell line (HepG2) cells were cultured on the quartz crystal through a collagen film. ...
Bao Ning - - 2008
Analysis of intracellular materials at the single-cell level presents opportunities for probing the heterogeneity of a cell population. Lysis by electroporation has been gaining popularity as a rapid method for disruption of the cell membrane and release of intracellular contents. In this report, we selectively released specific intracellular molecules for ...
Song Guanbin - - 2008
It has been proven that steep pulsed electric field (SPEF) can directly kill tumor cells and plays an important role in anticancer treatment. The biorheological mechanisms, however, that destroy tumor cells are almost unknown. To resolve this issue, here, an SPEF generator was used to assess the effects of high- ...
Mady Mohsen M - - 2009
It is necessary to understand liposomal uptake mechanisms and intracellular distribution in order to design more efficient gene (drug) carrier systems. Until now, a few studies have been carried out using confocal laser scanning microscopy (CLSM) to investigate the cellular uptake and transfection mediated with liposomes. So, by CLSM, we ...
Shudo, Toshio
Power generation with direct methanol fuel cell (DMFC) systems require only simple equipment, and has the important advantages of using a liquid fuel with higher energy density and easier handling characteristics than hydrogen. However, the power output of DMFC is lower than hydrogen fuel cells. To improve the power output ...
Kua Chin Hock - - 2008
Moving dielectrophoresis has been recently developed by the authors as an alternative method to achieve simultaneous cell fractionation and transportation. With an array of independently excitable microelectrodes, this method generates a moving electric field to sequentially fractionate and transport cells across a microchannel. Due to the peculiarity of this method, ...
Zhang Jin-Na - - 2008
A microbial fuel cell using aerobic microorganisms as the cathodic catalysts is described. By using anaerobic sludge in the anode and aerobic sludge in the cathode as inocula, the microbial fuel cell could be started up after a short lag time of 9 days, generating a stable voltage of 0.324 ...
Dumas Claire - - 2008
A procedure was proposed to mimic marine microbial fuel cell (MFC) in liquid phase. A graphite anode and a stainless steel cathode which have been proven, separately, to be efficient in MFC were investigated. A closed anodic compartment was inoculated with sediments, filled with deoxygenated seawater and fed with milk ...
Akagi Takanori - - 2008
An overview of both experimental and theoretical studies of cell electrophoresis mobility (EPM) over the past fifty years and the relevance of cell EPM measurement are presented and discussed from the viewpoint of exploring the potential use of cell EPM as an index of the biological condition of cells. Physical ...
Trontelj Katja - - 2008
Cell electrofusion is a phenomenon that occurs, when cells are in close contact and exposed to short high-voltage electric pulses. The consequence of exposure to pulses is transient and nonselective permeabilization of cell membranes. Cell electrofusion and permeabilization depend on the values of electric field parameters including amplitude, duration and ...
Debuisson Damien - - 2008
Nanoscale probes have been developed for the online characterization of the electrical properties of biological cells by dielectric spectroscopy. Two types of sensors have been designed and fabricated. The first one is devoted to low (<10 MHz) frequency range analysis and consists of gold nanoelectrodes. The second one works for ...
Celano M - - 2008
In this study, we evaluated the activity of two novel pyrazolopyrimidine derivatives (Si 34 and Si 35) against ARO cells, a human anaplastic thyroid cancer cell line. ARO cells exposed to different concentrations of the drugs showed a reduced growth rate and an increase of mortality. After 72 h incubation, ...
Pitts-Singer Theresa L - - 2008
Cavity-nesting alfalfa leafcutting bees, Megachile rotundata (F.) (Hymenoptera: Megachilidae), are excellent pollinators of alfalfa, Medicago savita L., for seed production. In commercial settings, artificial cavities are placed in field domiciles for nesting and, thereby, bee populations are sustained for future use. For this study, cells from leafcutting bee nests were ...
Fan Shih-Kang - - 2008
Two important electric forces, dielectrophoresis (DEP) and electrowetting-on-dielectric (EWOD), are demonstrated by dielectric-coated electrodes on a single chip to manipulate objects on different scales, which results in a dielectrophoretic concentrator in an EWOD-actuated droplet. By applying appropriate electric signals with different frequencies on identical electrodes, EWOD and DEP can be ...
Vykoukal Jody - - 2008
We have applied the microfluidic cell separation method of dielectrophoretic field-flow fractionation (DEP-FFF) to the enrichment of a putative stem cell population from an enzyme-digested adipose tissue derived cell suspension. A DEP-FFF separator device was constructed using a novel microfluidic-microelectronic hybrid flex-circuit fabrication approach that is scaleable and anticipates future ...
Xi Xiaoyu - - 2008
In our previous work, it was found that acoustic cavitation might play a role in improving the cell permeability to microparticles when liposomes were used in an in vitro experiment. The purpose of this project is to expand our study and to learn other possible mechanisms by which cells may ...
Bhattacharya Shantanu - - 2008
We present a novel, on-chip system for the electrokinetic capture of bacterial cells and their identification using the polymerase chain reaction (PCR). The system comprises a glass-silicon platform with a set of micro-channels, -chambers, and -electrodes. A platinum thin film resistor, placed in the proximity of the chambers, is used ...
Wang Lei - - 2008
Cell migration is crucial in many physiological and pathological processes including embryonic development, immune response and cancer metastasis. Traditional methods for cell migration detection such as wound healing assay usually involve physical scraping of a cell monolayer followed by an optical observation of cell movement. However, these methods require hand-operation ...
Novikova N S - - 2008
Studies of the pattern of activation of hypothalamic cells in conditions of electrical pain stimulation demonstrated an increase in the proportion of c-Fos-positive cells (to 72.02-98.95%), predominantly affecting cells of size 10-50 microm2. UHF irradiation of the skin activated hypothalamic cells of size 10-70 microm2 in the paraventricular and dorsomedial ...
Galland Marie-Annick - - 2008
Hybrid passiveactive cells previously developed at the LMFA have proved their efficiency for global noise reduction. A hybrid cell combines passive absorbent properties of a porous layer and active control at its rear face. Active control is mainly used to increase absorption at low frequencies by cancelling the imaginary part ...
Galindo Federico Gómez - - 2008
The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and ...
Choi D S - - 2008
We describe a method to induce hyperthermia in cells, in-vitro, by remotely heating Ni nanowires (NWs) with radio frequency (RF) electromagnetic fields. Ni NWs were internalized by human embryonic kidney cells (HEK-293). Only cells proximal to NWs or with internalized NWs changed shape on exposure to RF fields indicative of ...
Pavicic Ivan - - 2008
The aim of this study was to evaluate whether low-level, ultra high frequency (UHF) irradiation of 935 MHz influences the cell structure and growth of V79 cells. UHF field was generated inside a Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell) with a Hewlett-Packard signal generator. The electric field strength was 8.2+/-0.3 ...
Bhattarai Shanta Raj - - 2008
We developed hexanoyl chloride-modified chitosan (Nac-6) stabilized iron oxide nanoparticles (Nac-6-IOPs) as magnetic nanoparticles for viral gene (Ad/LacZ) delivery via magnetofection. This vector, Nac-6-IOPs/Ad/LacZ, binds to K562 cells in the presence of external magnetic fields and results in enhanced expression of the transgene in those cells that do not exhibit ...
Guignet Emmanuel G EG Department of Chemical and Systems Biology, Bio-X Program, Clark Center, 318 Campus Drive, Stanford University School of Medicine, Stanford, California 94305, USA. - - 2008
We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent ...
Seriburi Pahnit - - 2008
This work presents an electrical technique called electric cell-substrate impedance sensing to measure the cell-substrate separation and the projected area of an individual adherent cell. Cell adhesion and cell spreading are fundamental processes of adherent cells. By recording changes in the cell-substrate separation, the projected area, or both properties with ...
Xiao Xilin - - 2008
A wireless sensing device was developed for the in-situ monitoring of the growth of human breast cancer cells (MCF-7) and evaluation of the cytotoxicity of the anticancer drugs fluorouracil and cisplatin. The sensor is fabricated by coating a magnetoelastic ribbon-like sensor with a layer of polyurethane that protects the iron-rich ...
Chen Guo-Wei - - 2008
Instead of the utilization of artificial redox mediators or other catalysts, a biocathode has been applied in a two-chamber microbial fuel cell in this study, and the cell performance and microbial community were analyzed. After a 2-month startup, the microorganisms of each compartment in microbial fuel cell were well developed, ...
Cogle Christopher R - - 2008
The nascent field of regenerative medicine has taken root in cardiovascular disease. Preclinical data demonstrating hemangioblast potential of marrow cells and cardioprotective effects of growth factors served as the basis for numerous early phase clinical trials. With the first wave of safety and efficacy trials complete, much is still unknown ...
Kang Yuejun - - 2008
DC-Dielectrophoresis (DC-DEP), the induced motion of the dielectric particles in a spatially non-uniform DC electric field, is applied to separate biological cells by size. The locally non-uniform electric field is generated by an insulating hurdle fabricated within a PDMS microchannel. The cells experience a negative DEP (accordingly a repulsive) force ...
Busalmen Juan Pablo - - 2008
The mechanism(s) by which electricity-producing microorganisms interact with an electrode is poorly understood. Outer membrane cytochromes and conductive pili are being considered as possible players, but the available information does not concur to a consensus mechanism yet. In this work we demonstrate that Geobacter sulfurreducens cells are able to change ...
Tryfona Theodora - - 2008
The permeability barrier of the microbial cell envelope for substrates and products often causes very low reaction rates of whole cells. Therefore, it is of interest to develop an effective method to reduce this permeability barrier in order to increase product yields. Utilisation of pulse electric fields may improve amino ...
Le Duc Vinh - - 2008
We present a coupled immersed interface method-boundary element method (IIM-BEM) numerical technique that predicts the behaviour of deformable cells under the effect of both hydrodynamic and electrical forces. This technique is applied to the study of a hybrid electrical-mechanical trap for single-cell trapping. We report on the effect of different ...
Ron Amit - - 2008
Dielectric spectroscopy (DS) of living biological cells is based on the analysis of the complex dielectric permittivity of cells suspended in a physiological medium. It provides knowledge on the polarization-relaxation response of cells to external electric field as function of the excitation frequency. This response is strongly affected by both ...
Marc Paul J PJ Department of Biomedical Engineering, University of California, Irvine, California 92697, - - 2008
Electrically addressable cell traps were integrated with capillary electrophoresis for the analysis of the contents of single adherent cells. Electrodes composed of indium tin oxide were patterned on a glass surface followed by formation of topographical cell traps using 1002F photoresist. Single cells trapped in the holes could be lysed ...
Yasukawa Tomoyuki - - 2008
A novel microfluidic device with an array of analytical chambers was developed in order to perform single-cell-based gene-function analysis. A series of analytical processes was carried out using the device, including electrophoretic manipulation of single cells and electrochemical measurement of gene function. A poly(dimethylsiloxane) microstructure with a microfluidic channel (150 ...
Bao Ning - - 2008
Microfluidics offers an ideal platform to integrate cell-based assays with electric measurements. The technological advances in microfluidics, microelectronics, electrochemistry, and electrophysiology have greatly inspired the development of microfluidic/electric devices that work with a low number of cells or single cells. The applications of these microfluidic systems range from the detecting ...
Urdaneta Mario - - 2008
This paper presents a method of using multiple frequencies to counteract electric field distortions that interfere with the dielectrophoretic (DEP) manipulation of particles or cells. To demonstrate the technique, simulations were performed for a scenario in which cells were to be loaded into a cage whose walls created parasitic trapping ...
Zhu He - - 2008
The development of integrated microsystems capable of interrogation, characterization and sorting of mammalian cells is highly significant for further advancement of point-of-care diagnostics and drug discovery fields. The present study sought to design a novel strategy for releasing antibody-bound cells through electrochemical disruption of the underlying antibody (Ab) layer. A ...
Kjeang Erik - - 2008
A microfluidic fuel cell architecture incorporating flow-through porous electrodes is demonstrated. The design is based on cross-flow of aqueous vanadium redox species through the electrodes into an orthogonally arranged co-laminar exit channel, where the waste solutions provide ionic charge transfer in a membraneless configuration. This flow-through architecture enables improved utilization ...
Cricenti A - - 2008
Human HaCaT cells, exposed for 24 h to a 1 mT (rms) 50 Hz sinusoidal magnetic field in a temperature-regulated solenoid, suffer detectable changes in their biochemical properties and shapes. By using infrared wavelength-selective scanning near-field optical microscopy, we observed changes in the distribution of the inner chemical functional groups ...
Fukuda Nobuo - - 2008
Magnetic separation provides a relatively quick and easy-to-use method for cell isolation and protein purification. We have developed a rapid and efficient procedure to isolate yeast cells displaying a target polypeptide, namely, the Staphylococcus aureus ZZ domain, which serves as s model for protein interactions and can bind immunoglobulin G ...
Santagata Scott - - 2008
Despite the embryological and anatomical disparities present among lophotrochozoan phyla, there are morphological similarities in the cellular arrangements of ciliated cells used for propulsion among the nonfeeding larval forms of kamptozoans, nemerteans, annelids, mollusks, and bryozoans. Evaluating whether these similarities are the result of convergent selective pressures or a shared ...
< 4 5 6 7 8 9 10 11 12 13 14 >