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Results 251 - 300 of 1932
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Sinclair Michael S MS Program in Neurosciences, Department of Physiology & Biophysics, University of Miami Miller School of Medicine, Miami, Florida, United States of - - 2010
The neuropeptide, oxytocin (OXT), acts on brain circuits to inhibit food intake. Mutant mice lacking OXT (OXT knockout) overconsume salty and sweet (i.e. sucrose, saccharin) solutions. We asked if OXT might also act on taste buds via its receptor, OXTR. Using RT-PCR, we detected the expression of OXTR in taste ...
Lassance-Soares Roberta M RM Department of Physiology, The Johns Hopkins University, School of Medicine, Baltimore, MD, - - 2010
This study tested the hypotheses that the hypertonic environment of the renal medulla regulates the expression of cystic fibrosis transmembrane conductance regulator protein (CFTR) and its natural splice variant, TNR-CFTR. To accomplish this, Madin-Darby canine kidney (MDCK) stable cell lines expressing TNR-CFTR or CFTR were used. The cells were treated ...
Chen Hsiang-Chin HC Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey, - - 2010
TRPM7 is a ubiquitous divalent-selective ion channel with its own kinase domain. Recent studies have shown that suppression of TRPM7 protein expression by RNA interference increases resistance to ischemia-induced neuronal cell death in vivo and in vitro, making the channel a potentially attractive pharmacological target for molecular intervention. Here, we ...
Xiao Yan - - 2010
Trpp5 is one member of the polycystic kidney disease (PKD) family, which belongs to transient receptor potential (TRP) superfamily. Our previous study has shown that Trpp5 is developmentally expressed in mouse testis and overexpression of Trpp5 increases intracellular free calcium concentration in MDCK cells. However, the roles of this protein ...
Koshiba Taichi - - 2010
Boron (B) deprivation induces various responses in plant cells, some of which can be observed very early. However, it has been unknown what kind of signal is generated by the stress. We found that B deprivation induced the expression of stress-responsive genes within 1 h in suspension-cultured tobacco BY-2 cells. ...
Dando Robin - - 2009
Isolated taste cells, taste buds and strips of lingual tissue from taste papillae secrete ATP upon taste stimulation. Taste bud receptor (Type II) cells have been identified as the source of ATP secretion. Based on studies on isolated taste buds and single taste cells, we have postulated that ATP secreted ...
Dvoryanchikov Gennady - - 2009
Cells in taste buds are closely packed, with little extracellular space. Tight junctions and other barriers further limit permeability and may result in buildup of extracellular K(+) following action potentials. In many tissues, inwardly rectifying K channels such as the renal outer medullary K (ROMK) channel (also called Kir1.1 and ...
Antoniadou Maria - - 2009
Hybrid solar cells have been constructed by using nanocrystalline titania and hole-transporting polymers. Titania was deposited on fluorine-doped tin-oxide transparent electrodes in three layers: a blocking layer and two nanostructured layers, giving densely packed or open structures. Open structures produced higher currents due to better polymer penetration and larger oxide-polymer ...
Limbourg Anne - - 2009
Cell therapy is a promising approach to improve cardiac function in patients with ischemic heart disease. Beneficial effects of cell therapy have been shown in experimental studies and clinical trials. However, with current treatment strategies the therapeutic effect is limited. In the current article, critical aspects of cell therapy are ...
Sutiagin P V - - 2009
The presence of action potentials of working atrial cardiomyocytes among action potentials of true and latent pacemakers was revealed in the part of bidimentional maps of distribution of action potentials of sinoatrial node cells on the frontal surface of the right atrium under conditions of short-term culturing using intracellular glass ...
Yang Yang - - 2009
Bridges for treatment of the injured spinal cord must stabilize the injury site to prevent secondary damage and create a permissive environment that promotes regeneration. The host response to the bridge is central to creating a permissive environment, as the cell types that respond to the injury have the potential ...
Ubels John L - - 2010
The goal of this study was to determine whether prevention of K(+) loss can protect human corneal-limbal epithelial (HCLE) cells from UV-B induced apoptosis. Immunostaining for activated caspase-3 of HCLE cells exposed to 150-200 mJ/cm(2) UV-B demonstrated induction of apoptosis 6 h after exposure. The number of apoptotic cells was ...
Tian Li-Juan - - 2009
The formalin test is a commonly used animal model of acute and tonic pain. However, the molecular targets of formaldehyde (FA, the main ingredient of the formalin solution) on primary nociceptor cells remain controversial. In this report, the effects of FA on electrophysiologically-identified primary nociceptor cells were evaluated in vitro ...
Torisawa Yu-suke YS Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, - - 2009
This paper describes a microfluidic method to form co-culture spheroids of various geometries and compositions in order to manipulate cell-cell interaction dynamics. The cellular patterning is performed in a two-layered microfluidic device that sandwiches a semi-porous membrane so that flow occurs from the top channel through the membrane to the ...
Newton Gregory J - - 2009
Although members of the genus Shewanella have common features (e.g., the presence of decaheme c-type cytochromes [c-cyts]), they are widely variable in genetic and physiological features. The present study compared the current-generating ability of S. loihica PV-4 in microbial fuel cells (MFCs) with that of well-characterized S. oneidensis MR-1 and ...
Chandrashekar Jayaram - - 2009
Carbonated beverages are commonly available and immensely popular, but little is known about the cellular and molecular mechanisms underlying the perception of carbonation in the mouth. In mammals, carbonation elicits both somatosensory and chemosensory responses, including activation of taste neurons. We have identified the cellular and molecular substrates for the ...
Germanà A - - 2009
The Sox-2 is a transcription factor involved in adult neurogenesis in different vertebrate species, including fishes. Sox-2 also participates in growth and renewal on sensory cells in neuromasts of the fish lateral line system, and it is essential for development of taste buds in mammals. Using immunohistochemistry and Western blot ...
Grineva N I - - 2009
Cell regulation of Ph(+)cell proliferation and differentiation has been studied ex vivo in various chronic myeloid leukemia (CML) patients. The regulation is provided by alternation of effective stages of proliferation and maturation with inhibition of Ph(+) cell proliferation by accumulating neutrophils under apoptosis blockage. The alternation of stages consists of ...
Afrasiabi Emad - - 2010
The human ether-a-go-go related gene (HERG) potassium channel has elicited intense scientific interest due to its role in cardiac repolarization and its association with arrhythmia and sudden cardiac death. Increasing evidence indicates the involvement of HERG channels in the pathophysiology of cancer. In the present study we investigated the expression ...
Xu Tao - - 2010
High-throughput single cell analysis is required for understanding and predicting the complex stochastic responses of individual cells in changing environments. We have designed a microfluidic device consisting of parallel, independent channels with cell-docking structures for the formation of an array of individual cells. The microfluidic cell array was used to ...
Yamazaki M - - 2010
Taste dysfunction that develops after radiotherapy for head and neck cancer impairs patients' quality of life. Although taste cells have been shown to degenerate after exposure to X-ray irradiation, the alteration in taste cell population is unclear. This study investigated the histopathological change of taste bud structure and the taste ...
Donida Bianca Maria - - 2009
Casein phosphopeptides (CPPs), originating by in vitro and/or in vivo casein digestion, are characterized by the ability to complex and solubilize calcium ions preventing their precipitation. Previous works demonstrated that CPPs improve calcium uptake by human differentiated intestinal tumor cell lines, are able to re-mineralize carious lesions in a dental ...
Peng Xiong-Bo - - 2009
Double fertilization is a key process of sexual reproduction in higher plants. The role of calcium in the activation of female sex cells through fertilization has recently received a great deal of attention. The establishment of a Ca(2+)-imaging technique for living, single, female sex cells is a difficult but necessary ...
Chantome Aurelie - - 2009
Cell migration and invasion are required for tumour cells to spread from the primary tumour bed so as to form secondary tumours at distant sites. We report evidence of an unusual expression of KCa2.3 (SK3) protein in melanoma cell lines but not in normal melanocytes. Knockdown of the KCa2.3 channel ...
Pereira Bruno Fiorelini - - 2009
Chloride cells are responsible for ionic exchanges in the fish gill. These cells have been widely studied, considering its importance in vital functions of the gill, and because they proliferate when exposed to unfavorable environments. One of the main characteristic of these cells is an acidic cytoplasm, which has been ...
Calloe Kirstine K Danish National Research Foundation Center for Cardiac Arrhythmias, Department of Biomedical Sciences 12.5.10, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, - - 2010
To examine the electrophysiological and molecular properties of the transient outward current (I(to)) in canine left ventricle using a novel I(to) activator, NS5806, I(to) was measured in isolated epicardial (Epi), midmyocardial (Mid) and endocardial (Endo) cells using whole-cell patch-clamp techniques. NS5806 activation of K(v)4.3 current was also studied in CHO-K1 ...
Friis Ulla G - - 2009
The mechanism by which extracellular hypotonicity stimulates release of renin from juxtaglomerular (JG) cells is unknown. We hypothesized that osmotically induced renin release depends on water movement through aquaporin-1 (AQP1) water channels and subsequent prostanoid formation. We recorded membrane capacitance (C(m)) by whole-cell patch clamp in single JG cells as ...
Adachi Taiji - - 2009
It is proposed that osteocytes embedded in the bone matrix have the ability to sense deformation and/or damage to the matrix and to feed these mechanical signals back to the adaptive bone remodeling process. When osteoblasts differentiate into osteocytes during the bone formation process, they change their morphology to a ...
Yoshida Ryusuke R Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, - - 2009
Multiple lines of evidence from molecular studies indicate that individual taste qualities are encoded by distinct taste receptor cells. In contrast, many physiological studies have found that a significant proportion of taste cells respond to multiple taste qualities. To reconcile this apparent discrepancy and to identify taste cells that underlie ...
Iwatsuki Ken - - 2009
Taste cells are chemosensory epithelial cells that sense distinct taste qualities. It is the type II taste cell that express G-protein coupled receptors to sense either umami, sweet, or bitter compounds. Whereas several reports have suggested involvement of ATP in taste signal transduction, there is a paucity of molecular information ...
Chen James - - 2009
Calcium is a key component of the mineralized enamel matrix, but may also have a role in ameloblast cell differentiation. In this study we used human ameloblast lineage cells to determine the effect of calcium on cell function. Primary human ameloblast lineage cells were isolated from human fetal tooth buds. ...
Young Andrew - - 2009
Dynasore, a small molecule inhibitor of dynamin, was used to probe the role of dynamin in the endocytosis of wild-type and mutant CFTR (cystic fibrosis transmembrane conductance regulator). Internalization of both wild-type and 'temperature-corrected' DeltaF508 CFTR was markedly inhibited by a short exposure to dynasore, implicating dynamin as a key ...
Buckinx Roeland - - 2009
OLN-93 cells, a cell line established from spontaneously transformed rat brain glial cultures, are used as a model for oligodendrocytes. These cells are known to undergo morphological changes upon serum deprivation. The objective of the present study is to investigate a possible correlation between these morphological changes and (1) the ...
Ide Nobuyuki - - 2009
This study investigated the sweetness of the sweet-tasting protein thaumatin and lysozyme by both an in vitro cell-based assay and an in vivo sensory analysis to elucidate the differences between in vitro and in vivo response profiles. Hek293 cells were constructed that stably expressed the human T1R2+T1R3 sweet-taste receptor, and ...
Yamashita Daisuke - - 2009
The aim of this study was to evaluate the effect of surface roughness on the initial attachment of mouse osteoblast-like cells on ceriastabilized zirconia/alumina nanocomposite (NANOZR) and yttria-stabilized zirconia (3Y-TZP) in comparison to those on pure titanium (Ti) and alumina oxide (AO). Specimens with smooth and rough surfaces were prepared ...
Rivers David B - - 2009
The endoparasitoid Pimpla hypochondriaca injects venom during oviposition to condition its lepidopteran hosts. Venom is a complex mixture of proteins and polypeptides, many of which have been identified as enzymes, including phenoloxidase, endopeptidase, aminopeptidase, hydrolase, and angiotensin-converting enzyme. Constituents of the venom have been shown to possess cytolytic and paralytic ...
Ichimori Yasuo - - 2009
The present study was designed to examine the histochemical changes and occurrence of apoptosis in taste buds of rat circumvallate papillae following bilateral transection of the glossopharyngeal nerve. Following transection of the glossopharyngeal nerve, the number of taste buds was not altered until post-operative day 3 (PO3), but decreased significantly ...
Thirumangalathu Shoba S Department of Cell and Developmental Biology and the Rocky Mountain Taste & Smell Center, University of Colorado Denver School of Medicine, Aurora, Colorado 80045, USA. - - 2009
In mammals, the homogeneous lingual epithelium in the process of development forms specialized placodal cells that undergo a series of morphogenetic changes to form a papilla. Taste buds appear in the papillary epithelium around birth and thus papillae serve to house the taste buds in the adult. However, evidence for ...
Roper Stephen D SD Department of Physiology and Biophysics, and Program in Neuroscience, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA. - - 2009
Neuroscientists are now coming to appreciate that a significant degree of information processing occurs in the peripheral sensory organs of taste prior to signals propagating to the brain. Gustatory stimulation causes taste bud cells to secrete neurotransmitters that act on adjacent taste bud cells (paracrine transmitters) as well as on ...
Kapoor Niren - - 2009
High grade gliomas such as glioblastoma multiforme express multiple members of the epithelial sodium channel (ENaC)/Degenerin family, characteristically displaying a basally active amiloride-sensitive cation current not seen in normal human astrocytes or lower grade gliomas. Using quantitative real time PCR, we have shown higher expression of ASIC1, alphaENaC, and gammaENaC ...
Wareham K - - 2009
P2X receptors are widely expressed in cells of the immune system with varying functions. This study sought to characterize P2X receptor expression in the LAD2 human mast cell line and human lung mast cells (HLMCs). Reverse transcriptase polymerase chain reaction (RT-PCR) and patch clamp studies were used to characterize P2X ...
Majumdar Sriparna - - 2009
Glycine receptors (GlyRs) of displaced amacrine cells of the mouse retina were analysed using whole cell recordings and immunocytochemical staining with subunit-specific antibodies. During the recordings the cells were filled with a fluorescent tracer and 11 different morphological types could be identified. The studies were performed in wild-type mice and ...
Subbiah Jegadesan - - 2009
In this contribution, we report on bulk-heterojunction solar cells using a solution-processable neutral green conjugated copolymer based on 3,4-dioxythiophene and 2,1,3-benzothiadiazole as the donor and [6,6]phenyl-C61 butyric acid methyl ester (PCBM) as the acceptor. We have found that the short-circuit current is very sensitive to the composition of the donor-acceptor ...
Vallés Ana Sofía AS Instituto de Investigaciones Bioquímicas de Bahía Blanca and UNESCO Chair of Biophysics and Molecular Neurobiology, CC 857, B8000FWB, Bahía Blanca, - - 2009
Studies of the alpha7-type neuronal nicotinic acetylcholine receptor (AChR), one of the receptor forms involved in many physiologically relevant processes in the central nervous system, have been hampered by the inability of this homomeric protein to assemble in most heterologous expression systems. In a recent study, it was shown that ...
Thirumangalathu Shoba S Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, - - 2009
Mammalian taste buds have properties of both epithelial and neuronal cells, and are thus developmentally intriguing. Taste buds differentiate at birth within epithelial appendages, termed taste papillae, which arise at mid-gestation as epithelial thickenings or placodes. However, the embryonic relationship between placodes, papillae and adult taste buds has not been ...
Casas-Pruneda Griselda - - 2009
Mouse parotid acinar cells express P2X4 and P2X7 receptors (mP2X4R and mP2X7R) whose physiological function remains undetermined. Here we show that mP2X4R expressed in HEK-293 cells do not allow the passage of tetraethylammonium (TEA+) and promote little, if any, ethidium bromide (EtBr) uptake when stimulated with ATP or BzATP. In ...
Hirschfeld, Julian
A novel scheme for detecting inhomogeneous internal currents in a fuel cell stack is suggested. Plates of high conductivity, including small parallel slits, are placed between each fuel cell in a stack. These may consist of aluminum. In this diploma thesis the scheme is investigated for the case that the ...
Cosentino Stefania - - 2010
Caseinphosphopeptides (CPPs) are considered as mineral carriers because of their ability to bind and solubilize calcium ions, with the possible role, yet to be definitely assessed, of improving calcium absorption at the intestinal level. Previous works demonstrated that CPPs improve calcium uptake, with increasing intracellular calcium concentration, by human differentiated ...
Roper Stephen D - - 2009
ROPER, S.D. Parallel processing in mammalian taste buds? Physiol Behav XXX(Y) 000-000, 2009. There is emerging evidence that two parallel lines of gustatory information are generated in taste buds. One pathway leads to higher cortical centers and is involved in discriminating basic taste qualities (sweet, bitter, sour, salty, umami) and ...
Haggie Peter M PM 1246 Health Sciences East Tower, Box 0521, Univ. of California, San Francisco, San Francisco, CA 94143-0521, USA. - - 2009
The cellular mechanisms by which loss-of-function mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel produce cystic fibrosis (CF) lung disease remain uncertain. Defective organellar function has been proposed as an important determinant in the pathogenesis of CF lung disease. According to one hypothesis, reduced CFTR chloride conductance ...
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