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Results 201 - 250 of 1936
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Xu Shenghong - - 2010
In the present study, we subclassified acutely dissociated trigeminal ganglion (TRG) cells of rats using a current signature method in whole cell patch-clamp recordings. Using modified criteria for cell classification for the dorsal root ganglion (DRG), TRG cells were subclassified into nine cell types: 1-5, 7-9, and 13. Types 1, ...
Lee Chuan-Pei - - 2010
The effects of incorporation of various weight percentages of titanium carbide (TiC) into TiO(2) matrices on the photovoltaics of the respective dye-sensitized solar cells (DSSCs) were investigated. It is established through relevant photographs, XRD and EDX analysis that TiC was partially converted into anatase TiO(2) (a-TiO(2)) when the TiC was ...
Ehrensberger Mark T - - 2010
An electrochemically controlled system has been developed which allows for cell culture directly on electrically polarized metal surfaces with simultaneous control and assessment of the electrochemical current, potential, and impedance of the interface. This system was utilized in this study to assess the interactions between electrochemically polarized commercially pure titanium ...
Guilding C - - 2010
The habenula complex is implicated in a range of cognitive, emotional and reproductive behaviors, and recently this epithalamic structure was suggested to be a component of the brain's circadian system. Circadian timekeeping is driven in cells by the cyclical activity of core clock genes and proteins such as per2/PER2. There ...
Ishimaru Yoshiro Y Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. - - 2010
The polycystic kidney disease 1-like 3 (PKD1L3) and polycystic kidney disease 2-like 1 (PKD2L1) proteins have been proposed to form heteromers that function as sour taste receptors in mammals. Here, we show that PKD1L3 and PKD2L1 interact through their transmembrane domains, and not through the coiled-coil domain, by coimmunoprecipitation experiments ...
Esche Mirko - - 2010
Signalling of sphingosine-1-phosphate (S1P) via G-protein-coupled receptors of the Endothelial Differentiation Gene family differentially regulates cellular processes such as migration, proliferation and morphogenesis in a variety of cell types. Proliferation and migration of retinal Müller glial cells are involved in pathological events such as proliferative vitreoretinopathy and proliferative diabetic retinopathy. ...
Sommerhage F - - 2010
There is a continuously growing scientific and technological interest to develop and improve the application of artificial sensors. Biological components which are capable to transduce neutral signals into specific, robust and reproducible indicators frame an attractive alternative to construct biohybrid sensors. Since naturally "occurring" biosensors are only sparsely compatible with ...
Aslanidi Oleg V - - 2010
The intrinsic heterogeneity of electrical action potential (AP) properties between Purkinje fibers (PFs) and the ventricular wall, as well as within the wall, plays an important role in ensuring successful excitation of the ventricles. It can also be proarrhythmic due to nonuniform repolarization across the Purkinje-ventricular junction. However, the ionic ...
Murata Yoshihiro Y Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University, Higashi-ku, Fukuoka, - - 2010
Only some taste cells fire action potentials in response to sapid stimuli. Type II taste cells express many taste transduction molecules but lack well-elaborated synapses, bringing into question the functional significance of action potentials in these cells. We examined the dependence of adenosine triphosphate (ATP) transmitter release from taste cells ...
Ainla Alar - - 2010
We report on a free-standing microfluidic pipette made in poly(dimethylsiloxane) having a circulating liquid tip that generates a self-confining volume in front of the outlet channels. The method is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. ...
Yamada Takahiro - - 2010
OBJECTIVES: To investigate the functional expression of the transient receptor potential vanilloid 2 (TRPV2) channel protein in human urothelial carcinoma (UC) cells and to determine whether calcium influx into UC cells through TRPV2 is involved in apoptotic cell death. MATERIAL AND METHODS: The expression of TRPV2 mRNA in bladder cancer ...
Luciani Paola - - 2010
Glucagon-like peptide-1 (GLP-1) is an insulinotropic peptide with neurotrophic properties, as assessed in animal cell models. Exendin-4, a GLP-1 analogue, has been recently approved for the treatment of type 2 diabetes mellitus. The aim of this study was to morphologically, structurally, and functionally characterize the differentiating actions of exendin-4 using ...
Miquerol Lucile - - 2010
RATIONALE: The ventricular conduction system controls the propagation of electric activity through the heart to coordinate cardiac contraction. This system is composed of specialized cardiomyocytes organized in defined structures including central components and a peripheral Purkinje fiber network. How the mammalian ventricular conduction system is established during development remains controversial. ...
Sullivan Jeremy M - - 2010
Adult taste buds are maintained by the lifelong proliferation of epithelial stem and progenitor cells, the identities of which have remained elusive. It has been proposed that these cells reside either within the taste bud (intragemmal) or in the surrounding epithelium (perigemmal). Here, we apply three different in vivo approaches ...
Sato Toshihide - - 2010
Temperature sensitivity of frog taste cells was studied. The taste cell designated Type thermosensitive (TS) I cell was depolarized by warm stimulus at 30 degrees C and hyperpolarized by cold stimulus at 10 degrees C. The taste cell designated Type TS II cell was depolarized by the cold stimulus and ...
Lai Bin B Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, California 94945, - - 2010
The subventricular zone (SVZ) is a principal site of adult neurogenesis and appears to participate in the brain's response to injury. Thus, measures that enhance SVZ neurogenesis may have a role in treatment of neurological disease. To better characterize SVZ cells and identify potential targets for therapeutic intervention, we studied ...
Liang Zhiyin Z Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6058, - - 2010
In the vertebrate visual system, ON cells respond to positive contrasts and OFF cells respond to negative contrasts, and thus both ON and OFF cells exhibit rectification. We investigated the retinal circuits by which the ON pathway rectifies the OFF pathway. White noise was projected onto an in vitro preparation ...
Ajonuma Louis Chukwuemeka - - 2010
Chlamydia trachomatis is an obligate intracellular Gram-negative pathogen affecting over 600 million people worldwide with 92 million new cases occurring globally each year. C. trachomatis enter the cells and replicate to infect different tissues/organs, giving rise to a spectrum of pathological conditions; however, the exact mechanism or receptor(s) for their ...
Zhao Qinghui - - 2010
This study aims to investigate the interaction between calcium silicate/calcium phosphate cement (CS/CPC) and osteogenesis, in particular the in vitro osteoblast-like and endothelial cells' response to CS/CPC. The effect of CS/CPC on cell attachment, proliferation and differentiation of murine osteoblast-like cell MC3T3-E1, as well as the influence on the cell ...
Wang Tongguang T Department of Neurology, The Johns Hopkins University, Baltimore, Maryland 21287, - - 2010
There is a great need for pharmacological approaches to enhance neural progenitor cell (NPC) function particularly in neuroinflammatory diseases with failed neuroregeneration. In diseases such as multiple sclerosis and stroke, T-cell infiltration occurs in periventricular zones where NPCs are located and is associated with irreversible neuronal loss. We studied the ...
Ortega-Sáenz Patricia - - 2010
Background K(+) channels of the TASK family are believed to participate in sensory transduction by chemoreceptor (glomus) cells of the carotid body (CB). However, studies on the systemic CB-mediated ventilatory response to hypoxia and hypercapnia in TASK1- and/or TASK3-deficient mice have yielded conflicting results. We have characterized the glomus cell ...
Rodriguez Andrea P - - 2010
In recent years, calcium titanate (CaTiO(3)) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get "calcium titanate with calcium carbonate" (CaTiO(3)-CaCO(3)). The objective was to evaluate the crystallinity and elemental ...
Chiung Yin-Mei - - 2010
Occupational exposure to toluene diisocyanats (TDI) may cause asthma. In asthma patients, the allergic syndromes correlate cytokine production with the elevation in cytosolic calcium concentration [Ca(2+)](c) of lymphocytes in airway. We previously found TDI induces calcium signaling in neuronal cells. TDI mainly gets into human body via inhalation; therefore this ...
Huang Yuelong - - 2010
The performance of tubular microbial fuel cells (MFC) with and without Nafion solution as binding agent for the cathode catalyst preparation was investigated using different electrochemical techniques. The current output of both types of MFCs was monitored as a function of time using an external resistor. The current did not ...
Vukcevic Mirko - - 2010
Spontaneous Ca(2+) oscillations have been observed in a number of excitable and non-excitable cells, but in most cases their biological role remains elusive. In the present study we demonstrate that spontaneous Ca(2+) oscillations occur in immature human monocyte-derived dendritic cells but not in dendritic cells stimulated to undergo maturation with ...
Rowe S M SM Department of Medicine, University of Alabama at Birmingham, 1530 3rd Ave. South, Birmingham, AL 35294-0005, United States. - - 2010
We examined the activity of DeltaF508 cystic fibrosis transmembrane conductance regulator (CFTR) stably expressed in polarized cystic fibrosis bronchial epithelial cells (CFBE41o(-)) human airway cells and Fisher Rat Thyroid (FRT) cells following treatment with low temperature and a panel of small molecule correctors of DeltaF508 CFTR misprocessing. Corr-4a increased DeltaF508 ...
Klesing J - - 2010
The charge of nanoparticles influences their ability to pass through the cellular membrane, and a positive charge should be beneficial. The negative charge of calcium phosphate nanoparticles with an inner shell of carboxymethyl cellulose (CMC) was reversed by adding an outer shell of poly(ethyleneimine) (PEI) into which the photoactive dye ...
O'Brien P G - - 2010
Wave-optics analysis is performed to investigate the benefits of utilizing Bragg-reflectors and inverted ZnO opals as intermediate reflectors in micromorph cells. The Bragg-reflector and the inverted ZnO opal intermediate reflector increase the current generated in a 100 nm thick upper a-Si:H cell within a micromorph cell by as much as ...
Wongsamitkul Nisa - - 2010
PURPOSE: The present study examined the effects and mechanisms of actions of penta-m-digalloyl-glucose (PDG), a hydrolysable tannin extracted from Chinese gallnut, on cystic fibrosis transmembrane conductance regulator protein (CFTR). MATERIALS AND METHODS: Fisher rat thyroid cells stably expressing human CFTR (FRT cells) and human intestinal T84 cells were used as ...
Park Jung-Ae - - 2010
The aim of this pilot study was to investigate the effect of etched microgrooves on the hydrophilicity of Ti and osteoblast responses. Microgrooves were applied on Ti to have 15 and 60 µm width, and 3.5 and 10 µm depth by photolithography, respectively. Further acid etching was applied to create ...
Okuyama Tomoaki - - 2010
We describe the use of a microfluidic device to micropattern cells in a microchannel and investigated the behavior of these cells under a concentration gradient. The microfluidic device consisted of 3 parts: a branched channel for generating a stable concentration gradient, a main channel for culturing cells, and 2 side ...
Nobles Muriel - - 2010
An inwardly rectifying K(+) current is present in atrial cardiac myocytes that is activated by acetylcholine (I(KACh)). Physiologically, activation of the current in the SA node is important in slowing the heart rate with increased parasympathetic tone. It is a paradigm for the direct regulation of signaling effectors by the ...
Bystrova Marina F - - 2010
Three types of morphologically and functionally distinct taste cells operate in the mammalian taste bud. We demonstrate here the expression of two G-protein-coupled receptors from the family C, CASR and GPRC6A, in the taste tissue and identify transcripts for both receptors in type I cells, no transcripts in type II ...
LeSimple Pierre - - 2010
The cystic fibrosis transmembrane conductance regulator (CFTR) is an integral membrane glycoprotein which functions as an anion channel and influences diverse cellular processes. We studied its role in the development of epithelial tightness by expressing wild-type (WT-CFTR) or mutant (Delta F508-CFTR) CFTR in human airway epithelial cell monolayers cultured at ...
Sotthibandhu Pongsiwa - - 2010
There have been a number of studies which have categorized cells of feline taste buds: Types I, II, III and IV; however, few studies have examined whether feline taste bud cell types differ from each other histochemically. The goal of the present study is to figure out what kinds of ...
Richie-Jannetta Robyn R Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, - - 2010
2-Arachidonoylglycerol is oxygenated by cyclooxygenase-2 to form prostaglandin glyceryl esters. Previous work in this laboratory has suggested that PGE(2)-G activates a novel G protein-coupled receptor in a murine macrophage-like cell line, RAW 264.7. To probe the structural determinants for the putative receptor in RAW 264.7 cells, a panel of 10 ...
Han Yi - - 2010
Cardiac c-kit(+) cells are generally believed to be the major population of stem/progenitor cells in the heart and can be used as a cell source for cardiomyoplasty; however, the cellular electrophysiological properties are not understood in this type of cells. The present study was designed to investigate functional ion channels ...
Liu Pei-Shan - - 2010
Butyl benzyl phthalate (BBP), an endocrine disruptor present in the environment, exerts its genomic effects via intracellular steroid receptors and elicits non-genomic effects by interfering with membrane ion-channel receptors. We previously found that BBP blocks the calcium signaling coupled with P2X receptors in PC12 cells (Liu & Chen, 2006). Osteoblast ...
Bertrand J - - 2010
One of the major therapeutic strategy in cystic fibrosis aims at developing modulators of cystic fibrosis transmembrane conductance regulator (CFTR) channels. We recently discovered methylglyoxal alpha-aminoazaheterocycle adducts, as a new family of CFTR inhibitors. In a structure-activity relationship study, we have now identified GPact-11a, a compound able not to inhibit ...
Montechiaro Federico - - 2010
In the CO2-richer world that awaits us, the impact of elevated pCO2 on the allocation of resources in phytoplankton may have profound repercussions on the physiology of the microalgae and on the ecology of the ecosystems of which they are part. We studied the overall physiology and cell composition of ...
Hartveit Espen - - 2010
Accurate measurement of the junctional conductance (G(j)) between electrically coupled cells can provide important information about the functional properties of coupling. With the development of tight-seal, whole-cell recording, it became possible to use dual, single-electrode voltage-clamp recording from pairs of small cells to measure G(j). Experiments that require reduced perturbation ...
Wongsamitkul Nisa - - 2010
PURPOSE: The present study examined the effects and mechanisms of actions of penta-m-digalloyl-glucose (PDG), a hydrolysable tannin extracted from Chinese gallnut, on cystic fibrosis transmembrane conductance regulator protein (CFTR). MATERIALS AND METHODS: Fisher rat thyroid cells stably expressing human CFTR (FRT cells) and human intestinal T84 cells were used as ...
Pedemonte Nicoletta - - 2010
Cystic fibrosis (CF) is caused by mutations in the CFTR chloride channel. Deletion of phenylalanine 508 (F508del), the most frequent CF mutation, impairs the maturation and gating of the CFTR protein. Such defects may be corrected in vitro by pharmacological modulators named as correctors and potentiators, respectively. We have evaluated ...
Meng Xin - - 2010
Measles virus Edmonston strain (MV-Edm) is thought to have remarkable oncolytic activity that selectively destroys human tumor cells. The P/V/C protein of wild-type MV was shown to resist the antiviral effects of interferon (IFN)-alpha. Here, we engineered new MVs by arming MV-Edm tag strain (a V-defective vaccine-lineage strain, MV-Etag) with ...
Steiner Florian - - 2010
Ion channels are membrane proteins that gate the flow of ions into and out of a cell. They are present in the membranes of human, animal, plant, and bacterial cells. They are profoundly involved in diverse tasks ranging from neuronal functions to hormonal secretion and cell division. Biophysical characterization and ...
Abou Neel Ensanya A - - 2010
This study challenged to produce phosphate-based glasses (PBG) for the treatment of osseous defects. The glasses contained, among other components, 40 mol% CaO and 1-5 mol% TiO(2). The mechanical performance and in vitro biocompatibility using both human osteosarcoma and primary osteoblasts were carried out. Incorporation of TiO(2) into PBG had no significant ...
Iordan Andreea - - 2010
Collagen model tissues, consisting of cells embedded in a collagen matrix at different concentrations (of cells and collagen) were analyzed. Rheological properties were measured and complementary confocal microscopy analysis carried out. An important feature, corresponding to the breakdown of the collagen network (i.e., decrease in network elasticity) was observed at ...
Sun Yubo - - 2010
INTRODUCTION: Calcium crystals exist in the knee joint fluid of up to 65% of osteoarthritis (OA) patients and the presence of these calcium crystals correlates with the radiographic evidence of hyaline cartilaginous degeneration. This study sought to examine calcium deposition in OA meniscus and to investigate OA meniscal cell-mediated calcium ...
Yoshida Ryusuke - - 2010
Taste receptor cells detect chemical compounds in the oral cavity and transfer their messages to gustatory afferent nerve fibers. Considering the coding of taste information, the sensitivity of taste cells and the connection between taste cells and gustatory fibers may be critical in this process. Broadly tuned taste cells and ...
Quian Quiroga Rodrigo - - 2010
The current authors reply to a response by Bowers on a comment by the current authors on the original article. A typical problem in any discussion about grandmother cells is that there is not a general consensus about what should be called as such. Here, we discuss possible interpretations in ...
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