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Ma Liang L Department of Anorectal Surgery, The Affiliated Traditional Chinese Medicine Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. - - 2014
In this study, the effect of emodin and its mechanism of action were investigated in LOVO colorectal cancer cells. Cell growth was determined using a Cell Counting kit-8 assay, and the results demonstrated that emodin significantly inhibited the growth of LOVO cells in a concentration-dependent manner. In order to investigate ...
Chen Yuanyuan Y State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Shanghai, China (Y.C., D.M., D.W., Y.Z., J.W., C.H., X.S., D.Y., L.H., J.Y., H.C., H.C., D.L.); Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, China (J.F.); Department of Neurosurgery, (H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Eighth Department of General Surgery and Department of Pathology, First Affiliated Hospital of Anhui Medical University, Hefei, China (R.S., S.Y.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Neurosurgery Research Institution of Shanghai, Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, China (R.Q., T.X., - - 2014
Malignant glioma is a common and lethal primary brain tumor in adults. Here we identified a novel oncoprotein, vesicle-associated membrane protein 8 (VAMP8), and investigated its roles in tumorigenisis and chemoresistance in glioma. The expression of gene and protein were determined by quantitative PCR and Western blot, respectively. Histological analysis ...
Marques Cláudia N H CN Binghamton Biofilm Research Center (BBRC), Binghamton University, Binghamton, NY, 13902 - - 2014
Persister cells are cells tolerant to antimicrobials that contribute to the biofilm recalcitrance to therapeutic agents. In turn, the ability to kill persister cells is believed to significantly improve efforts in eradicating biofilm-related, chronic infections. While much research has focused on elucidating the mechanism(s) by which persister cells form, little ...
Topcuoglu Nursen - - 2014
Purpose: To evaluate the antibacterial effect of Kenger gum on mutans streptococci (in vivo) and Streptococcus mutans (in vitro) and its cytotoxic effect on the 3T3 fibroblast cell line. Materials and Methods: In vitro antibacterial activity of Kenger gum extracts against S.mutans was determined by the disk-diffusion method. The broth ...
Maeno Atsushi A Department of Urology, Kyoto University Graduate School of Medicine, Kyoto, - - 2014
MicroRNAs are noncoding small RNA that negatively regulate target gene expression by binding to the 3'-UTR of mRNA. Previous studies have shown that several microRNAs play a pivotal role in prostate cancer by acting as oncogenes or tumor suppressors. This study was aimed at identifying microRNAs that contribute to the ...
Lee Young Ju YJ Department of Urology, Seoul National University Bundang Hospital, Seongnam-si, Seoul, Republic of - - 2014
Cisplatin-based chemotherapy is the first‑line treatment for metastatic urothelial cell carcinoma. However, for cisplatin‑resistant cases, no chemotherapeutic agent has been established as a standard of treatment. This study aimed to investigate the synergistic antitumor effect of ginsenoside Rg3 on cisplatin in cisplatin‑resistant bladder cancer cells (T24R2). T24R2 cells were treated ...
Zong Can C Department of Ultrasound, Shengjing Hospital, China Medical University, Shenyang, 110001, People's Republic of China, - - 2014
MicroRNAs (miRNAs) have recently been identified as a novel class of gene regulators, playing an important role in various malignancies. In the present study, we investigated the role of miRNA-130b in the development of drug resistance in ovarian cancer cells. The human ovarian carcinoma cell line A2780 and paclitaxel-resistant A2780/Taxol ...
Chueca Beatriz B Tecnología de los Alimentos, Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, - - 2014
Oxygenated monoterpenes citral and carvacrol are common constituents of many essential oils (EOs) that have been extensively studied as antimicrobial agents but whose mechanisms of microbial inactivation have not been totally elucidated. A recent study described a mechanism of Escherichia coli death for (+)-limonene, a hydrocarbon monoterpene also frequently present ...
Li Zhenqing Z Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China. Electronic address: - - 2014
Gingivitis is a highly prevalent periodontal disease around the worldwide. Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic ...
Rocha Thiago Lopes TL CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, - - 2014
There is an increased use of Quantum Dot (QDs) in biological and biomedical applications, but little is known about their marine ecotoxicology. So, the aim of this study was to investigate the possible immunocytotoxic, cytogenotoxic and genotoxic effects of cadmium telluride QDs (CdTe QDs) on the marine mussel Mytilus galloprovincialis. ...
Song Young-Gyun YG Department of Prosthodontics, College of Dentistry, Dankook University, Cheonan, Republic of - - 2014
The purpose of this study was to evaluate the properties of a porous zirconia scaffold coated with bioactive materials and compare the in vitro cellular behavior of MC3T3-E1 preosteoblastic cells to titanium and zirconia disks and porous zirconia scaffolds. Titanium and zirconia disks were prepared. A porous zirconia scaffold was ...
Zhang Jingjing J Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, - - 2014
Injectable hydrogel plays an important role in soft tissue filling and repair. We report an injectable hydrogel based on hyaluronic acid (HA) and human-like collagen (HLC), both with favorable biocompatibility and biodegradability. These two types of biomacromolecules were crosslinked with 1,4-butanediol diglycidyl ether to form a three-dimensional network. The redundant ...
Dias N J NJ Department of Biological Sciences, Duquesne University, Pittsburgh, PA, - - 2014
Estrogen plays an important role in maintaining bone density. Postmenopausal women have low plasma estrogen, but have high levels of conjugated steroids, particularly estrone sulfate (E1S) and dehydroepiandrosterone sulfate (DHEAS). Conversion of these precursors to active estrogens may help maintain bone density in postmenopausal women. The enzyme steroid sulfatase (STS) ...
Garewal Jessica J Senior Lecturer, Department of Oral Pathology and Microbiology, National Dental College and Hospital , Derabassi, India - - 2014
Neoplasia results from various genetic and epigenetic factors. Our study focused on the pathogenesis which involved an imbalance in various molecular mechanisms which regulated cell proliferation and apoptosis. The anti-apoptotic mechanism is regulated by Bcl-2 gene, while Ki-67 is expressed exclusively in nuclei of proliferating cells. This study was done ...
Amirzada Muhammad Imran MI Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, PR China. Electronic address: - - 2014
The major cause of multidrug resistance is over-expression of membrane P-glycoprotein (P-gp). We investigated the effect of recombinant human interleukin 24 (rhIL-24) on the Adriamycin (ADM)-resistant human breast cancer cell line MCF-7/ADM. The cytotoxicity of rhIL-24 and ADM was determined by 3-[4,5-dimethylthiazol-2-yl], 5-diphenyl tetrazolium bromide (MTT) assays. The expression of ...
Xing L L Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, - - 2014
The radial glial cell (RGC) is a glial cell type in the central nervous system of all vertebrates. Adult teleost fish have abundant RGCs in the brain in contrast to mammals. Adult fish RGCs have many important functions, including forming a structural scaffold to guide neuronal migration and serving as ...
El Bitar Fadia F INSERM U788 and Université Paris SUD, Faculté de Médecine, UMR-S788, 94276, Le Kremlin-Bicêtre, - - 2014
Pregnenolone sulfate (PREGS) and dehydroepiandrosterone sulphate (DHEAS) are pro-amnesic, anti-amnesic and neuroprotective steroids in rodents. In Alzheimer's disease (AD) patient's brains, their low concentrations are correlated with high levels of Aβ and tau proteins. The unnatural enantiomer ent-PREGS enhanced memory in rodents. We investigated here whether ent-PREGS and ent-DHEAS could ...
Schwabe Anne A Systems Bioinformatics, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The - - 2014
Individual cells respond very differently to changes in environmental conditions. Stochasticity causes cells to respond at different times, magnitudes or both. Here we disentangle and quantify these two sources of heterogeneity. We track the adaptation dynamics of single Saccharomyces cerevisiae cells exposed to a nutrient shift from methionine to sulphate ...
Robertson Scott M SM Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of - - 2014
In the 4-cell C. elegans embryo, a signal from P2 to its anterior sister, EMS, specifies the posterior daughter of EMS, E, as the sole founder cell for intestine. The P2-to-EMS signal restricts high level zygotic expression of the redundant GATA transcription factors, END-1 and END-3, to only the E ...
Fernandes Ana C AC INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. - - 2014
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes ...
Gutiérrez Diana D Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, - - 2014
Staphylococcal biofilms are a major concern in both clinical and food settings because they are an important source of contamination. The efficacy of established cleaning procedures is often hindered due to the ability of some antimicrobial compounds to induce biofilm formation, and to the presence of persister cells, a small ...
Wu Xiaonan X Department of Periodontology, Hospital of Stomatology, Medical School of Nanjing University, Nanjing, People's Republic of - - 2014
Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, type I collagen/poly(ε-caprolactone) (COL/PCL) and type ...
Kaneko Masahiro M Departments of Animal Sciences and Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, JAPAN, - - 2013
Nucleoside transport is important for nucleic acid synthesis in cells that cannot synthesize nucleosidesde novo, and for entry of many cytotoxic nucleoside analog drugs used in chemotherapy.This study demonstrates that various steroid hormones induce inhibition of nucleoside transport in mammalian cells. We analyzed the inhibitory effects of estradiol (E2) on ...
Magaña-Vergara Nancy E - - 2013
We describe the synthesis of steroidal dendrimer conjugates of first and second generation with tetramethylene core and 5-hydroxy-isophtalic acid dimethyl ester as branching unit modified to incorporate ethynylestradiol or 17α-estradiol as terminal units. The steroidal dendrimer conjugates, the free drug (steroids) and dendrimer were tested against a panel of cancer ...
Tian Hai-Yan - - 2013
Five new C23 steroids (1-5) together with five known bufadienolides (6-10) were isolated from the venom of Bufo bufo gargarizans (ChanSu in Chinese). The structures of the new steroids were elucidated by extensive spectroscopic methods in combination with X-ray diffraction analysis. Among these C23 steroids, only compound 3 showed cytotoxicities ...
Iida S S Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, - - 2013
Steroid sulphatase (STS) is one of the steroid-metabolising enzymes involved in desulphating inactive steroid sulphates and oestrogen sulphotransferase (EST) sulphates active oestrogen. The roles of both STS and EST have not been examined in oestrogen-dependent non-small-cell lung cancer (NSCLC). We evaluated the immunoreactivity of STS and EST in NSCLC cases ...
Palyanova Natalia V - - 2013
The neuritogenic and neuroprotective activities of six starfish polar steroids, asterosaponin Р1, (25S)-5α-cholestane-3β,4β,6α,7α,8,15α,16β,26-octaol, and (25S)-5α-cholestane-3β,6α,7α,8,15α,16β,26-heptaol (1-3) from the starfish Patiria pectinifera and distolasterosides D1-D3 (4-6) from the starfish Distolasterias nipon were analyzed using the mouse neuroblastoma (NB) C-1300 cell line and an organotypic rat hippocampal slice culture (OHSC). All of ...
Ajeawung Norbert Fonya - - 2012
Pediatric low grade gliomas are the most common central nervous system tumors and are still incurable among a subset of patients despite current treatment modalities. Steroid biosynthesis occurs in a wide variety of organs including the brain, to mediate an assortment of functions, including a proposed role in the growth ...
Buaas F William - - 2012
Adrenal and gonadal steroids are essential for life and reproduction. The orphan nuclear receptor SF1 (NR5A1) has been shown to regulate the expression of enzymes involved in steroid production in vitro. However, the in vivo role of this transcription factor in steroidogenesis has not been elucidated. In this study, we ...
Zhang Bei-Bei - - 2012
Phytochemical investigation of the ethanol extract obtained from the aerial parts of the Euphorbia altotibetic PAULS. grown in China resulted in the isolation of three new cholestane-type and three new ergostane-type steroids (cholest-5-en-2β, 4β-diol; cholest-5-en-1β, 4β-diol; cholest-5-en-1α, 3β, 4 α -triol; (22E)-ergosta-7,9,22-trien- 3β-ol β-D-glucoside; 5α-methoxy-(22E)-ergosta-7,9,22-trien-3β-ol β-D-glucoside; 6β- methoxy-(22E)-ergosta-7,9,22-trien-3β-ol β-D-glucoside), along ...
García-Sánchez Tomás - - 2012
In situ electroporation of adherent cells provides significant advantages with respect to electroporation systems for suspension cells, such as causing minimal stress to cultured cells and simplifying and saving several steps within the process. In this study, a new electrode assembly design is shown and applied to in situ electroporate ...
Khan M Nadeem - - 2012
Streptococcus pneumoniae (pneumococci) adhere to human nasopharyngeal (NP) epithelial cells as a first step in colonization and adherence of pneumococci to lung epithelia may be required to establish pneumonia. We sought to determine if PcpA can serve as an adhesin to human NP (D562) and lung (A549) epithelial cells and ...
Berillo Dmitriy - - 2012
Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin as a third component into the structure results in the formation of mesopores in the pore walls, which are not seen if gelatin is excluded. The mesoporous structure is explained by the ...
Konar Monica - - 2012
Enteroaggregative Escherichia coli (EAEC) adherence to human intestinal tissue is known to be mediated by aggregative adherence fimbriae (AAF). However, the host cell molecules involved in EAEC adherence remain uncharacterized. In the present study, four key membrane glycoproteins of cultured human intestinal epithelial cells (INT-407) were identified, which were found ...
Bible Amber - - 2012
The Che1 chemotaxis-like pathway of Azospirillum brasilense contributes to chemotaxis and aerotaxis, and it has also been found to contribute to regulating changes in cell surface adhesive properties that affect the propensity of cells to clump and to flocculate. The exact contribution of Che1 to the control of chemotaxis and ...
Othman Sarah - - 2012
A Pasteurellamultocida B:2 strain from a case of bovine haemorrhagic septicaemia (HS) and a derivative, JRMT12, that was attenuated by a deletion in the aroA gene, were shown to adhere to, invade and survive within cultured epithelial bovine lung (EBL) cells. By comparison, bovine strains of Mannheimia haemolytica serotype A1 ...
Kudva Indira T - - 2012
Abstract The aims of this study were to determine whether Shigella species, which are human gastrointestinal pathogens, can adhere to cattle recto-anal junction squamous epithelial (RSE) cells using a recently standardized in vitro adherence assay, and to compare their adherence patterns with that of Escherichia coli O157. Shigella dysenteriae (serogroup ...
Goodwin Arthur H - - 2012
A majority of states now restrict teenagers from using a mobile communication device while driving. The effect of these restrictions is largely unknown. In a previous study, we found North Carolina's teenage driver cell phone restriction had little influence on young driver behavior four months after the law took effect ...
Khan M Nadeem - - 2012
We evaluated the role of vaccine candidate surface proteins, PhtD and PhtE as antigens with functional importance for Streptococcus pneumoniae (pneumococci) in adherence to nasopharyngeal (D562) and lung (A549) epithelial cell lines. Comparing TIGR4 to PhtD and PhtE- isogenic mutants, a 40% (p=0.001) and 42% (p=0.002) drop in the number ...
Sayed Enas Taha - - 2012
The catalytic activity of baker's yeast, Saccharomyces cerevisiae, as a biocatalyst was investigated in a mediatorless microbial fuel cell. The yeast cells that adhered on the anode surface were the active biocatalyst for glucose oxidation in a mediatorless biofuel cell, suggesting that the electron transfer took place through the surface ...
Li Shi-Fei - - 2012
A phytochemical work on the alkaloid constituents from Trigonostemon lii Y.T. Chang was conducted to give six new β-carboline alkaloids, trigonostemines A-F (1-6) and eight known β-carboline alkaloids (7-14). Their structures were elucidated by extensive spectroscopic techniques including 2D NMR experiments and mass spectrometry. All of the compounds were evaluated ...
Saxena Sudhanshu - - 2012
Honey, both unifloral (Syzygiumcumini) and bifloral, demonstrated strong antimutagenicity against physical (UV, γ) and chemical (ethylmethane sulfonate) mutagens as ascertained by rpoB/Rif(R) and Ames tests. The effect of honey was evaluated in radiation (UV or γ) exposed Escherichia coli cells for SOS response, a well known error prone repair pathway ...
Haughn George W - - 2012
Arabidopsis seed coat epidermal cells produce a large quantity of mucilage that is extruded upon exposure to water. Chemical analyses and cell biological techniques suggest that this mucilage represents a specialized type of secondary cell wall composed primarily of pectin with lesser amounts of cellulose and xyloglucan. Once extruded, the ...
Vasseur Paule - - 2011
The Detailed Review Paper (DRP) number 31 of the Organisation for Economic Co-operation and Development (OECD) analyses the performance of the three models used in Cell Transformation Assays (CTAs) to screen the carcinogenic potential of chemicals: the Syrian hamster embryo (SHE) cells, and the mouse cell lines BALB/c 3T3 and ...
Jacquet N - - 2011
Perfluorooctane sulfonate (PFOS) is the degradation product of many fluoroderivatives and a widespread environmental contaminant. Its persistence, its long half-life in humans and its toxicity explain high concerns on human health side effects in future. PFOS is suspected to be a non-genotoxic carcinogen. In the present work, we assessed carcinogenic ...
Deng Hong - - 2011
V79-hCYP2E1-hSULT1A1, a V79-derived cell line co-expressing both human CYP2E1 and SULT1A1, has been constructed and efficiently used in detection of the mutagenic activities of a number of promutagens. 2-Nitropropane (2-NP) and N-nitrosodimethylamine (NDMA), both being hepatocarcinogenic to animals but inactive in standard genotoxicity assays in vitro, are activated to mutagenic ...
Sakai Ayako - - 2011
The Bhas 42 cell transformation assay is a sensitive short-term system for predicting chemical carcinogenicity. Bhas 42 cells were established from BALB/c 3T3 cells by the transfection of v-Ha-ras gene and postulated to have acquired an initiated state in the two-stage carcinogenesis theory. The Bhas 42 cell transformation assay is ...
Di Giorgio Carole - - 2011
The mutagenicity and clastogenicity of a series of 18 3,6-di-substituted acridines were evaluated by the Ames test on Salmonella typhimurium TA 97a and by the micronucleus assay on Chinese Hamster Ovary cells (CHO cells), as compared to their cytotoxicity against CHO cells. Experimental results overall demonstrated that simple symmetric molecules ...
Melzer Marvin S - - 2011
This commentary was prompted by many reports that carcinogenesis involving various carcinogens and various types of systems is blocked by inhibitors of endolysosomal proteases (i.e. leupeptin, antipain). These findings have been largely ignored by cancer investigators so far. In this commentary based on these reports a carcinogenesis model is suggested ...
Hirata Hiroshi - - 2011
The functional significance of Wnt antagonist DKK1 has not been investigated in renal cell carcinoma (RCC). Therefore, we hypothesized that DKK1 may be a tumor suppressor gene and is epigenetically silenced, thus decreased DKK1 may cause progression of RCC. To assess the function of DKK1, we established stable DKK1 transfected ...
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