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Chauveau Sabine S GReD , INSERM 1103, UMR CNRS 6293, Clermont Université, BP 38, 28 Place Henri-Dunant, Clermont-Ferrand, 63001, France, - - 2015
During the last decade it has been shown that most mammalian blastocysts consisted of three cell lineages. Immunofluorescence with multiple antibodies enables to identify each cell type allowing an easy detection of eventual defects. It is complementary to RT-PCR experiments as this technique allows to look at cell position and ...
Gisina A M AM V. N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia, - - 2014
Flow cytometry measurement of the expression of surface marker CD133 simultaneously with the analysis of fluorescent dye exclusion was performed in order to develop new methods for detection of cancer stem cell populations in tumor tissue samples from patients with colorectal adenocarcinoma. No correlation was found between the count of ...
Oprea-Lager Daniela E DE Department of Radiology and Nuclear Medicine, VU University Medical Center, PO Box 7057, 1007, MB, Amsterdam, The - - 2014
The objective of the present study is to determine whether uptake of [(18)F]fluoromethylcholine ([(18)F]FCH) in comparison with 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) accurately reflects chemotherapy efficacy at the tumor cell level in prostate cancer (PC). The effects of docetaxel and cabazitaxel on viable tumor cell number were explored in four PC cell lines. ...
Arnold Jill E JE National Aquarium, Baltimore, MD, - - 2014
Total blood cell counts for fish are challenging, especially due to cell fragility. Thrombocyte aggregation, cell distortion, and lysis can occur within hours of collection; therefore, hemocytometer counts may not be possible for large sample numbers. Preserving whole blood in formalin offers a simple way to extend the specimen stability ...
Walkling-Ribeiro Markus M Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON, Canada, N1G 2W1; Department of Food Science, Cornell University, Ithaca, NY, USA, - - 2014
Pulsed electric fields (PEF), heat-assisted PEF (H-PEF), and virulent bacteriophage (VP) are non-thermal techniques for pathogen inactivation in liquids that were investigated individually, and in combination (PEF/VP, H-PEF/VP) to control enterhaemorrhagic Escherichia coli (EHEC) O157:H7 in Luria-Bertani broth (LBB) and Ringer's solution (RS). Treated cells were subsequently incubated at refrigeration ...
Morii Takeshi T Department of Orthopaedic Surgery, Faculty of Medicine, Kyorin University, Mitaka, Tokyo, Japan - - 2014
Chondrosarcoma is refractory to conventional chemotherapy. BH-3 mimetics ABT-737 and ABT-263 are synthetic small-molecule inhibitors of anti-apoptotic proteins B-cell lymphoma-2 (Bcl2) and Bcl-xL, which play a critical role in survival of chondrosarcoma cells. Chondrosarcoma cell lines SW-1353 and CS-1 were used as the disease model. We used immunoblotting to assess ...
Sui Dong - - 2014
The Baculovirus Expression Vector System (BEVS) is a very popular expression vector system in gene engineering. An effective host cell line cultivation protocol can facilitate the baculovirus preparation and following experiments. However, the counting of the number of host cells in the protocol is usually performed by manual observation with ...
Xu Yang Y Chinese PLA Medical School, Beijing 100853, P.R. - - 2014
The aim of the current study was to observe the effects of suppressor of cytokine signaling 1 (SOCS1) silencing in human melanoma cells on cell biological behavior and interferon‑γ (IFN‑γ) sensitivity, and to investigate the use of SOCS1 as a therapeutic target in the treatment of melanoma. Western blot analysis ...
Grassby Terri T King's College London, Diabetes and Nutritional Sciences Division, Biopolymers Group, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK. - - 2014
The cell walls (dietary fibre) of edible plants, which consist of mainly non-starch polysaccharides, play an important role in regulating nutrient bioaccessibility (release) during digestion in the upper gastrointestinal tract. Recent studies have shown that structurally-intact cell walls hinder lipid release from the parenchyma cells of almond seeds. A theoretical ...
Liu Guanghong G Gynaecology and Obstetrics Department, Zhengzhou People's Hospital, 33 Huanghe Road, Zhengzhou, 450000, China, - - 2014
To investigate regulatory effects and associated mechanisms of myeloid differentiation factor 88 (MyD88) on cisplatin chemoresistance in human ovarian cancer (OC) cells. The expression of MyD88 in SKOV3/DDP cells was restrained by short interfering RNA (siRNA) of MyD88. Cell viability and proliferation in the presence or absence of cisplatin were ...
Ling Sunbin S Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. - - 2014
Metformin exhibits anti‑proliferative effects in tumor cells in vitro and in vivo. The present study investigated the ability of metformin to reverse multidrug resistance (MDR) in human hepatocellular carcinoma Bel‑7402/5‑fluorouracil (5‑Fu; Bel/Fu) cells. The synergistic anti‑proliferative effect of metformin combined with 5‑Fu was evaluated using a Cell Counting kit‑8 assay. The variation ...
Fallon Ann M AM Department of Entomology, University of Minnesota, 1980 Folwell Ave., St. Paul, MN 55108. Electronic address: - - 2014
Wolbachia is an obligate intracellular bacterium (Anaplasmataceae, Rickettisales) that occurs in arthropods and filarial worms, and spreads by vertical transmission in the oocyte cytoplasm. In insects, reproductive distortions associated with Wolbachia, such as cytoplasmic incompatibility in mosquitoes, have potential value for controlling pests, including species that transmit human, animal and ...
Ma Liang L Department of Anorectal Surgery, The Affiliated Traditional Chinese Medicine Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, P.R. - - 2014
In this study, the effect of emodin and its mechanism of action were investigated in LOVO colorectal cancer cells. Cell growth was determined using a Cell Counting kit-8 assay, and the results demonstrated that emodin significantly inhibited the growth of LOVO cells in a concentration-dependent manner. In order to investigate ...
Filippone Alfonso A Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 25, 71122, Foggia, - - 2014
In this paper, the bioactivity of cell-free extracts obtained by dairy propionibacteria strains was investigated. Probiotic bifidobacteria and lactobacilli were used as microbial targets. The extracellular filtrates were added as ingredient (1% v/v) into the growth medium; the effect of cell-free filtrates was evaluated through viable count of microbial targets ...
Guo Jiansheng J Department of Molecular and Cell Biology, School of Life Science and Technology, Tongji University, Shanghai, 200092, - - 2014
During asexual plant reproduction, cells from different organs can be reprogrammed to produce new individuals, a process that requires the coordination of cell cycle reactivation with the acquisition of other cellular morphological characteristics. However, the factors that influence the variety of asexual reproduction have not yet been determined. Here, we ...
Yang Guobin G The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, - - 2014
Dental papilla cells (DPCs) are precursors of odontoblasts and have the potential to differentiate into odontoblasts. Osteoblasts and odontoblasts have many common characteristics. Osterix (Osx) is essential for osteoblast differentiation. However, no information is available for the effects of Osx on the odontoblastic differentiation of DPCs. The purpose of this ...
Yang Jing J Graduate School, Hebei Medical University, Shijiazhuang, 050017, - - 2014
Thymoquinone (TQ) is the primary bioactive component of Nigella sativa Linn seed oil and used as anti-inflammatory, anti-oxidant, and anti-neoplastic agent. Previous studies have shown that TQ exhibits inhibitory effects on multiple cancers. However, the detailed antineoplastic effects and its molecular mechanisms of TQ on lung cancer are not entirely ...
Zhu Youlong Y Department of First General Surgery, Shanghai Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, P.R. - - 2014
CD133 has been reported to be associated with chemoresistance in various cancer cells. The efficacy of 5-fluorouracil (5-FU), an important chemotherapeutic agent for advanced gastric cancer (GC), is limited by 5-FU resistance. Hence, the present study investigated the function of CD133 in 5-FU resistance in human GC cells. We isolated ...
Rizzi M M Innovative Research Laboratory on Wound Healing, Health Sciences Department, University of Eastern Piedmont, Novara, 28100, - - 2014
Textiles used to make clothing can represent a source, often ignored, of chemicals potentially noxious to both skin and the whole organism. Among the most frequently produced potentially noxious chemical manufacturing by-products are formaldehyde (FA), nickel (Ni) and hexavalent chromium (Cr); they are of potential clinical interest as all are ...
Chen Yuanyuan Y State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Shanghai, China (Y.C., D.M., D.W., Y.Z., J.W., C.H., X.S., D.Y., L.H., J.Y., H.C., H.C., D.L.); Department of Biosynthesis, School of Pharmacy, Fudan University, Shanghai, China (J.F.); Department of Neurosurgery, (H.W.); Department of Hematology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China (S.W.); Eighth Department of General Surgery and Department of Pathology, First Affiliated Hospital of Anhui Medical University, Hefei, China (R.S., S.Y.); Department of Molecular Human Genetics, Baylor College of Medicine, Houston, Texas (X.Z.); Neurosurgery Research Institution of Shanghai, Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, China (R.Q., T.X., - - 2014
Malignant glioma is a common and lethal primary brain tumor in adults. Here we identified a novel oncoprotein, vesicle-associated membrane protein 8 (VAMP8), and investigated its roles in tumorigenisis and chemoresistance in glioma. The expression of gene and protein were determined by quantitative PCR and Western blot, respectively. Histological analysis ...
Marques Cláudia N H CN Binghamton Biofilm Research Center (BBRC), Binghamton University, Binghamton, NY, 13902 - - 2014
Persister cells are cells tolerant to antimicrobials that contribute to the biofilm recalcitrance to therapeutic agents. In turn, the ability to kill persister cells is believed to significantly improve efforts in eradicating biofilm-related, chronic infections. While much research has focused on elucidating the mechanism(s) by which persister cells form, little ...
Topcuoglu Nursen - - 2014
Purpose: To evaluate the antibacterial effect of Kenger gum on mutans streptococci (in vivo) and Streptococcus mutans (in vitro) and its cytotoxic effect on the 3T3 fibroblast cell line. Materials and Methods: In vitro antibacterial activity of Kenger gum extracts against S.mutans was determined by the disk-diffusion method. The broth ...
Maeno Atsushi A Department of Urology, Kyoto University Graduate School of Medicine, Kyoto, - - 2014
MicroRNAs are noncoding small RNA that negatively regulate target gene expression by binding to the 3'-UTR of mRNA. Previous studies have shown that several microRNAs play a pivotal role in prostate cancer by acting as oncogenes or tumor suppressors. This study was aimed at identifying microRNAs that contribute to the ...
Lee Young Ju YJ Department of Urology, Seoul National University Bundang Hospital, Seongnam-si, Seoul, Republic of - - 2014
Cisplatin-based chemotherapy is the first‑line treatment for metastatic urothelial cell carcinoma. However, for cisplatin‑resistant cases, no chemotherapeutic agent has been established as a standard of treatment. This study aimed to investigate the synergistic antitumor effect of ginsenoside Rg3 on cisplatin in cisplatin‑resistant bladder cancer cells (T24R2). T24R2 cells were treated ...
Dulugiac Magda M Regina Maria Central Stem Cells Bank, 5B Ion Ionescu de la Brad, 13811, Bucharest, Romania; Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, R-050095, Bucharest, - - 2014
Cell viability is an important indicator for the quality of umbilical cord blood (UCB) units that can influence the transplant final outcome. Thus, it is particularly important to identify the factors that may affect the cell quality during the banking process. The present study is a first attempt to correlate ...
Zong Can C Department of Ultrasound, Shengjing Hospital, China Medical University, Shenyang, 110001, People's Republic of China, - - 2014
MicroRNAs (miRNAs) have recently been identified as a novel class of gene regulators, playing an important role in various malignancies. In the present study, we investigated the role of miRNA-130b in the development of drug resistance in ovarian cancer cells. The human ovarian carcinoma cell line A2780 and paclitaxel-resistant A2780/Taxol ...
Chueca Beatriz B Tecnología de los Alimentos, Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, - - 2014
Oxygenated monoterpenes citral and carvacrol are common constituents of many essential oils (EOs) that have been extensively studied as antimicrobial agents but whose mechanisms of microbial inactivation have not been totally elucidated. A recent study described a mechanism of Escherichia coli death for (+)-limonene, a hydrocarbon monoterpene also frequently present ...
Li Zhenqing Z Engineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China. Electronic address: - - 2014
Gingivitis is a highly prevalent periodontal disease around the worldwide. Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic ...
Rocha Thiago Lopes TL CIMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, - - 2014
There is an increased use of Quantum Dot (QDs) in biological and biomedical applications, but little is known about their marine ecotoxicology. So, the aim of this study was to investigate the possible immunocytotoxic, cytogenotoxic and genotoxic effects of cadmium telluride QDs (CdTe QDs) on the marine mussel Mytilus galloprovincialis. ...
Song Young-Gyun YG Department of Prosthodontics, College of Dentistry, Dankook University, Cheonan, Republic of - - 2014
The purpose of this study was to evaluate the properties of a porous zirconia scaffold coated with bioactive materials and compare the in vitro cellular behavior of MC3T3-E1 preosteoblastic cells to titanium and zirconia disks and porous zirconia scaffolds. Titanium and zirconia disks were prepared. A porous zirconia scaffold was ...
Zhang Jingjing J Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, - - 2014
Injectable hydrogel plays an important role in soft tissue filling and repair. We report an injectable hydrogel based on hyaluronic acid (HA) and human-like collagen (HLC), both with favorable biocompatibility and biodegradability. These two types of biomacromolecules were crosslinked with 1,4-butanediol diglycidyl ether to form a three-dimensional network. The redundant ...
Dias N J NJ Department of Biological Sciences, Duquesne University, Pittsburgh, PA, - - 2014
Estrogen plays an important role in maintaining bone density. Postmenopausal women have low plasma estrogen, but have high levels of conjugated steroids, particularly estrone sulfate (E1S) and dehydroepiandrosterone sulfate (DHEAS). Conversion of these precursors to active estrogens may help maintain bone density in postmenopausal women. The enzyme steroid sulfatase (STS) ...
Garewal Jessica J Senior Lecturer, Department of Oral Pathology and Microbiology, National Dental College and Hospital , Derabassi, India - - 2014
Neoplasia results from various genetic and epigenetic factors. Our study focused on the pathogenesis which involved an imbalance in various molecular mechanisms which regulated cell proliferation and apoptosis. The anti-apoptotic mechanism is regulated by Bcl-2 gene, while Ki-67 is expressed exclusively in nuclei of proliferating cells. This study was done ...
Zhang Qiaoyin Q 1Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University, Yichang, Hubei 443002, - - 2014
Trametenolic acid B (TAB) is the main active composition of Trametes lactinea (Berk.) Pat which possesses antitumor activities. There was no report its antitumor effect through regulating P-glycoprotein (P-gp) so far, due toP-gp over expression is one of the most important mechanisms contributing to the multiple drug resistance phenotype. The ...
Amirzada Muhammad Imran MI Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, PR China. Electronic address: - - 2014
The major cause of multidrug resistance is over-expression of membrane P-glycoprotein (P-gp). We investigated the effect of recombinant human interleukin 24 (rhIL-24) on the Adriamycin (ADM)-resistant human breast cancer cell line MCF-7/ADM. The cytotoxicity of rhIL-24 and ADM was determined by 3-[4,5-dimethylthiazol-2-yl], 5-diphenyl tetrazolium bromide (MTT) assays. The expression of ...
Xing L L Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, - - 2014
The radial glial cell (RGC) is a glial cell type in the central nervous system of all vertebrates. Adult teleost fish have abundant RGCs in the brain in contrast to mammals. Adult fish RGCs have many important functions, including forming a structural scaffold to guide neuronal migration and serving as ...
El Bitar Fadia F INSERM U788 and Université Paris SUD, Faculté de Médecine, UMR-S788, 94276, Le Kremlin-Bicêtre, - - 2014
Pregnenolone sulfate (PREGS) and dehydroepiandrosterone sulphate (DHEAS) are pro-amnesic, anti-amnesic and neuroprotective steroids in rodents. In Alzheimer's disease (AD) patient's brains, their low concentrations are correlated with high levels of Aβ and tau proteins. The unnatural enantiomer ent-PREGS enhanced memory in rodents. We investigated here whether ent-PREGS and ent-DHEAS could ...
Schwabe Anne A Systems Bioinformatics, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The - - 2014
Individual cells respond very differently to changes in environmental conditions. Stochasticity causes cells to respond at different times, magnitudes or both. Here we disentangle and quantify these two sources of heterogeneity. We track the adaptation dynamics of single Saccharomyces cerevisiae cells exposed to a nutrient shift from methionine to sulphate ...
Robertson Scott M SM Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of - - 2014
In the 4-cell C. elegans embryo, a signal from P2 to its anterior sister, EMS, specifies the posterior daughter of EMS, E, as the sole founder cell for intestine. The P2-to-EMS signal restricts high level zygotic expression of the redundant GATA transcription factors, END-1 and END-3, to only the E ...
Wu Xiaonan X Department of Periodontology, Hospital of Stomatology, Medical School of Nanjing University, Nanjing, People's Republic of - - 2014
Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, type I collagen/poly(ε-caprolactone) (COL/PCL) and type ...
Gutiérrez Diana D Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, - - 2014
Staphylococcal biofilms are a major concern in both clinical and food settings because they are an important source of contamination. The efficacy of established cleaning procedures is often hindered due to the ability of some antimicrobial compounds to induce biofilm formation, and to the presence of persister cells, a small ...
Du Li L Department of Hematology, The Affiliated Hospital of Medical College, Qingdao University, 16 Jiangsu Road, Qingdao, Shandong 266003, - - 2014
Graphene oxide (GO) is a hotspot, especially in the field of biomedical. However, the clinical application of GO is still faces a lot of challenges. In order to improve the solubility and biocompatibility of GO, polyethylene glycol (PEG) was grafted on the surface of graphene oxide by amide reaction. PEGylated ...
Oliveira Tatiane T Department of Bioregulation, Institute of Health Sciences, Federal University of Bahia, 41110-100 Salvador, BA, Brazil ; Department of Biological and Diagnostic Sciences-Preventive Dentistry, University of Toronto, Toronto, ON, Canada M5G - - 2014
Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) ...
Fernandes Ana C AC INESC-MN-Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Rua Alves Redol 9, 1000-029 Lisbon, Portugal. - - 2014
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes ...
Li N N INRA, UMR 1253, Science et Technologie du Lait et de l'Œuf, 65 rue de Saint Brieuc, F-35042 Rennes, France ; Agrocampus Ouest, UMR 1253, Science et Technologie du Lait et de l'Œuf, 65 rue de Saint Brieuc, 35042 Rennes, France ; Actalia, BP 50915, 35009 Rennes, Cedex - - 2014
Somatic cells are an important component naturally present in milk, and somatic cell count is used as an indicator of udder health and milk quality. The role of somatic cells in dairy processes and products is ill-defined in most studies because the role of these cells combines also the concomitance ...
Li Wen-Ru WR State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, - - 2014
Litsea cubeba oil is extracted from the fresh fruits of Litsea cubeba by distillation. In this study, its chemical constituents, antibacterial activity, kinetics and effects against Escherichia coli were studied. Its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were both 0.125% (v/v) by toxic food method. Moreover, the ...
Jeppesen Dennis K DK Department of Molecular Medicine, Aarhus University Hospital, Aarhus, - - 2014
Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ in size, density and composition. The standard isolation method for exosomes is centrifugation of fluid samples, typically at 100,000×g or above. Knowledge of the effect of discrete ultracentrifugation speeds on the purification from different cell types, however, is ...
Wright Petra C PC Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27412 - - 2014
Carbon nanodots (CD), a new class of carbon nanomaterials with sizes below 10 nm, have recently attracted wide attention due to their superiority in water solubility, chemical inertness, and resistance to photobleaching. As a result, CD has found important and wide applications in energy, catalysis, biological labeling, bioimaging and drug ...
Kaneko Masahiro M Departments of Animal Sciences and Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, JAPAN, - - 2013
Nucleoside transport is important for nucleic acid synthesis in cells that cannot synthesize nucleosidesde novo, and for entry of many cytotoxic nucleoside analog drugs used in chemotherapy.This study demonstrates that various steroid hormones induce inhibition of nucleoside transport in mammalian cells. We analyzed the inhibitory effects of estradiol (E2) on ...
Magaña-Vergara Nancy E - - 2013
We describe the synthesis of steroidal dendrimer conjugates of first and second generation with tetramethylene core and 5-hydroxy-isophtalic acid dimethyl ester as branching unit modified to incorporate ethynylestradiol or 17α-estradiol as terminal units. The steroidal dendrimer conjugates, the free drug (steroids) and dendrimer were tested against a panel of cancer ...
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