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Scheumann G F - - 1996
There are several theories about the physiologic regeneration of adrenals and maintenance of physiologic steroid secretion after subtotal loss of adrenal cortical cells. According to the cell migration theory, adrenocytes from the zona glomerulosa migrate centripetally toward the medulla. This theory is opposed by the zonal theory according to which ...
Cahill A L - - 1996
Chromaffin cells were isolated from bovine adrenal glands and fractionated into two distinct subpopulations by density gradient centrifugation on Percoll. Cells in the more dense fraction stored epinephrine (E) as their predominant catecholamine (81% of total catecholamines), contained high levels of phenylethanolamine N-methyltransferase (PNMT) activity, and exhibited intense PNMT immunoreactivity. ...
Feinstein R E - - 1996
We estimated the total number of calcitonin-immunoreactive C-cells in rat thyroid gland using the optical fractionator, the unbiased stereological method for estimation of numbers. It was necessary first to use a fixative composed of formalin, acetic acid, and ethanol to distinctly visualize the C-cells. The 40-microm-thick sections had to adhere ...
Kibble A V - - 1996
We have examined the effect of alpha-SNAP on exocytosis in adrenal chromaffin cells by direct assay of exocytosis using patch-clamp capacitance analysis. Cells were recorded using the whole cell patch-clamp configuration and the cells dialysed with control pipette solution or with a pipette solution containing alpha-SNAP or the deletion mutant ...
Hirano T - - 1996
Effects of immobilization stress and hypophysectomy on the uptake of [3H] 2-deoxy-D-glucose (2DG) in the adrenal medulla of the mouse was examined by autoradiography. Radioactivity due to [3H]2DG in both adrenaline-storing and noradrenaline-storing cells was markedly increased by acute immobilization stress. Under immobilization stress, adrenaline-storing cells incorporated more 2DG than ...
Gustavsson B - - 1996
The effect of the human TSH-receptor (TSHR) on the growth of human anaplastic thyroid carcinoma cells lacking the endogenous expression of TSHR, was studied both in vitro and in vivo in NMRI nude mice. Cells from a human anaplastic thyroid carcinoma cell line (C643) were transfected with a TSHR cDNA, ...
Kagami H - - 1996
'Bakumondo-to', a Chinese-Japanese herbal medicine, has been used for patients with xerostomia in Japan. Although the efficacy of this medicine for xerostomia has been reported, the pharmacological basis was only partially understood. The aim of this study was to clarify the direct effect of Bakumondo-to on salivary gland cells using ...
Vollmer R R - - 1996
The innervation of the adrenal medulla regulates the release of catecholamines from the two, epinephrine (EPI) and norepinephrine (NE), populations of chromaffin cells. Adjustments in the neural output to the adrenal medulla are made by centers in the brain that integrate the sensory input arising from a variety of challenges ...
Date I - - 1996
Following polymer-encapsulation, human nerve growth factor-secreting baby hamster kidney fibroblasts (BHK-hNGF) were implanted into the striatum of hemiparkinsonian rats together with unencapsulated adrenal medullary chromaffin cells from either young (2 weeks) or old (12 months) donor rats. Animals receiving both BHK-hNGF cells and chromaffin cells exhibited significant decreases (39-56%) in ...
Chasserot-Golaz S - - 1996
Annexin II is a Ca(2+)-dependent membrane-binding protein present in a wide variety of cells and tissues. Within cells, annexin II is found either as a 36-kD monomer (p36) or as a heterotetrameric complex (p90) coupled with the S-100-related protein, p11. Annexin II has been suggested to be involved in exocytosis ...
Krause G E - - 1996
The recent establishment of an immortalized clonal cell line of rat parotid acinar cells (2RSG) by transfecting isoproterenol-stimulated parotid cells with a plasmid vector, pSV3neo which carries the large T-antigen gene from SV40 virus, afforded the opportunity to develop a model for parotid acinar cell transplantation. Single cell suspensions of ...
Igawa T - - 1996
By an indirect immunofluorescence method with In-111 cells (hamster insulinoma cell line), circulating islet cell surface antibodies (ICSA) were detected in 7 (20%) out of 36 patients with non-insulin dependent diabetes mellitus (NIDDM), 9% of 68 chronic thyroiditis (CT) patients, or 16% of 19 NIDDM patients associated with CT, but ...
Date I - - 1996
Encapsulated cell grafting is one approach for the delivery of neurotransmitters and/or neurotrophic factors to the brain. Baby hamster kidney (BHK) cells were genetically modified to secrete high levels of human nerve growth factor (hNGF). Following polymer encapsulation, these cells were implanted into the left lateral ventricle or the left ...
Wax T - - 1996
The salivary glands are the site of many neoplasms characterized by a mixture of epithelial and myoepithelial cells and stomal fragments. Cytologic distinction of these neoplasms may be very difficult but careful attention to cellular details and the interaction between the cells and stroma can correctly classify these neoplasms as ...
Lopez J M - - 1996
The morphology, frequency, and distribution of mitotic cells in the epithelium of the Harderian gland was studied in the male and female hamster from birth to the ninetieth day of postnatal life using light and electron microscopic techniques. The results obtained show that there is a gradual decline in the ...
Krause W - - 1996
Bovine chromaffin cells were separated by density gradient centrifugation into subfractions. After centrifugation on a self-generating Percoll gradient (42.75% isotonic Percoll, 30,000 x g for 22 min at 20 degrees C), the chromaffin cells were found in two clearly distinguishable peaks. The peak with the lower density contained most of ...
Elisei R - - 1996
A cell line derived from the Fisher rat thyroid (FRT), that does not have functional TSH receptor, was stably transfected with the cDNA of the human TSH receptor (h TSH-R). In wild FRT cells TSH (1-1000 mU/l) was unable to increase cAMP production, while 10-10000 nmol/l forskolin elicited a 10-30 ...
Serrano T - - 1996
We have studied the secretory goblet-cells of the salivary gland of six species of Helicoidea: Elona quimperiana, Trissexodon constrictus, Hygromia limbata, Cernuella aginnica, Cepaea nemoralis and Helix aspersa, using light microscopy and transmission electron microscopy. In each of the species studied, we have been able to demonstrate the presence of ...
Abelli L - - 1996
Histology demonstrated chromaffin cells in the head kidney and opisthonephros of the sea bass. Immunohistochemistry showed the catecholamine-synthesizing enzymes tyrosine hydroxylase and dopamine beta-hydroxylase in these cells. Phenlethanolamine N-methyltransferase was detected in a fraction of the chromaffin cells in the head kidney (59%) and in the opisthonephros (54%). Distinct noradrenaline- ...
Fernandez-Suarez A - - 1996
The fourth week of postnatal life is a critical point in the development of the hamster Harderian gland. During this week, cells with large lipid vacuoles (type-II cells) appear in the male gland, marking a morphological sex difference that is notorious in adult animals. The origin and fate of type-II ...
Bocian-Sobkowska J - - 1996
Stereological studies were performed on 27 pairs of adrenal glands of human fetuses (9-38 weeks of intra-uterine development). Medullary chromaffin cells were identified by immunostaining for chromogranin-A. The volume of adrenal medulla, average cell volume, and the number of chromaffin cells were calculated. The volume of adrenal medulla increased slowly ...
Petitfrere E - - 1996
In porcine thyroid cells in primary culture, thyrotropin (TSH) provokes important morphological changes associated with specific thyroidal function recovery. TSH influences cell-cell interactions and follicular morphogenesis. In the present report, we identify an ectotyrosine phosphatase activity controlled by chronic treatment with TSH. Specific substrates and various inhibitors allowed us to ...
Fayet G - - 1996
Hexamethylenebisacetamide (HMBA) provokes in murine erythroleukemia cells (MELC) a commitment to terminal differentiation leading to the activation of the expression of hemoglobin. HMBA has been tested also in other cells from colon cancer, melanoma or lung cancer. However it has not yet been tested in the thyroid. We demonstrate in ...
Unsicker K - - 1996
Chromaffin cells, the neuroendocrine cells of the adrenal medulla and paraganglia, occupy paradigmatic roles in molecular, cellular and developmental neurobiology. The fact that they are very effective 'minipumps', secreting numerous bioactive substances including amines, neuropeptides and proteins, has made them interesting and useful for the treatment of chronic pain and ...
Lesouhaitier O - - 1996
The adrenal gland of mammals contains high concentrations of peripheral-type benzodiazepine receptors (PBR) and diazepam-binding inhibitor (DBI), a polypeptide which acts as an endogenous ligand for PBR. The aim of the present study was to investigate the localization of DBI and PBR in the adrenal gland of the frog Rana ...
Suzuki T - - 1996
BACKGROUND: The adrenal medulla is a typical paraganglion, having the same origin as the sympathetic ganglia, and contains at least two types of parenchymal cells: chromaffin cells and supporting cells. We previously reported that the extent of cellular association of chromaffin cells with supporting cells was remarkably higher in noradrenaline ...
Czech K A - - 1996
Adrenal chromaffin cells have been utilized for several neural grafting applications, but limitations in allogeneic donor availability and dangers inherent in autografting limit the widespread use of this approach clinically. While xenogeneic donors offer promise as a source for cell transplantation in the central nervous system (CNS), immunologic responses to ...
Perry A - - 1996
BACKGROUND: Although hyperplasia of C-cells has been described in association with various pathologic and physiologic conditions, criteria for its diagnosis are poorly defined. Both neoplastic and physiologic C-cell proliferations have been lumped together under the umbrella designation of C-cell hyperplasia (CCH), creating considerable confusion among clinicians and pathologists. METHODS: in ...
Wang X T - - 1996
1. Preproenkephalin (PPEnk) mRNA expressing cells have been identified in rat pineal gland using radioactive in situ hybridization histochemistry. 2. Approximately 7% of the cells in the pineal gland (7.5 +/- 0.86, mean +/- 95% CI) express PPEnk mRNA. These cells are distributed throughout the pineal as either scattered single ...
Kasamo H - - 1996
The ontogeny of proliferative cells in the rat fundic gland was studied using bromodeoxyuridine (BrdU) immunohistochemistry from day 17.5 of gestation to 8 weeks after birth. This ontogenic process is divided into 4 stages. (1) The late fetal period extending to 0 day of birth: Proliferative cells were scattered throughout ...
Galera-Ruiz H H Department of Pathology, University of Seville, - - 1996
To determine if there are subvisual differences (quantitation of DNA and study of chromatin texture) that distinguish single-gland parathyroid disease (adenoma) from multiple-gland disease (hyperplasia). Forty-eight parathyroid glands from 41 patients with primary hyperparathyroidism were studied. Cytometric differences between the chief cells and clear cells were sought. An image cytometer ...
Hohenwarter O - - 1996
An in vitro bioassay for the biological action of thyroid hormones has been developed. The test is based on a dose-dependent increase in growth rate and cell activation of the rat pituitary tumor cell line GH3. This cell line is dependent on thyroid hormones for cell division when plated at ...
Lemullois M - - 1996
The secretion of proteins and fluids from the exorbital lacrymal gland of rat is mainly controlled by muscarinic receptors. In a recent pharmacological study. Mauduit et al (Am J Physiol (1993) 264, C1550-C1560) identified a homogeneous population of M3 muscarinic receptors in preparations of acini from these tissues. In order ...
Tanaka Y - - 1996
Oxyphil cell function in secondary parathyroid hyperplasia due to chronic renal failure was evaluated using in situ hybridization and heterotransplantation of parathyroid tissue. In situ hybridization and histologic analysis were performed on continuous frozen sections using 22 parathyroid tissues. A restricted area composed exclusively of oxyphil cells was observed in ...
Papanek P E - - 1996
Hypoxia in vivo leads to a decrease in aldosterone not completely explained by extrinsic controllers of adrenal function including adrenocorticotrophic hormone, renin-angiotensin II, and K+. The dissociation of renin and aldosterone during acute hypoxia in vivo may be explained by the finding that aldosterone synthesis in adrenal cells is reversibly ...
O'Hare M M - - 1996
The biosynthesis of neuropeptide Y (NPY) was investigated to determine the efficiency of synthesis and processing of the precursor. In brain tissues examined, the major product was amidated NPY(1-36). Although this was also the major product in adrenal and heart atrium, a minor portion of the immunoreactivity was identified as ...
Tischler Arthur S. - - 1996
Clues to mechanisms regulating development and tumorigenesis may be provided by studies of unusual diseases. Beckwit-Wiedemann syndrome (BWS) is a rare congenital disorder apparently related to abnormal regulation of insulin-like growth factor-2 (IGF-2) production. IGF2 mRNA has been previously localized to the chief cells of extra-adrenal paraganglia and to adult, ...
Sobis H - - 1996
A possible relationship between the development of granulated metrial gland (GMG) cells and trophoblast was studied. Trophoblast implanted in ectopic sites (e.g. kidney capsule) did not induce decidua and did not recruit GMG cells. Only when injected in utero did trophoblast lead to the development of decidua and to the ...
Peel S - - 1996
Antibody-dependent cellular cytotoxic (ADCC) activity of cells in rat metrial glands at days 13 and 20 of pregnancy has been examined. A 51chromium-release cytotoxicity assay was carried out using Yac-1 cells and P815 cells as targets in the presence of the corresponding, heat inactivated rat antibody or non-immune rat serum. ...
Grosso L E - - 1996
Previous morphologic and histochemical studies have documented extracellular elastin and elastic fibers within the matrix of pleomorphic adenomas of the salivary glands. By morphologic criteria, the elastin appeared to be synthesized by the tumor rather than being a consequence of stroma produced in response to the tumor cells. To examine ...
Taniguchi K - - 1996
The olfactory organs are equipped with their own associated glands, Bowman's glands (BG) of the olfactory epithelium (OE), and Jacobson's glands (JG) of the vomeronasal organ (VNO). Histology and ultrastructure of these glands in the adult were investigated in the Japanese reddish frog, Rana japonica, along with their development from ...
Sunardhi-Widyaputra S - - 1996
Parathyroid hormone-related peptide (PTHrP) has been shown to be produced as an early step in the differentiation sequence and is considered to be a marker of some progenitor cells. We investigated the presence and distribution of PTHrP in 7 normal parotid glands and in 18 salivary pleomorphic adenomas (PA). Localisation ...
Kimura H - - 1996
Using a syngeneic Wistar rat model we have shown that the Wistar rat thyroid (WRT) cell line causes significant and specific proliferation of lymph node T cells from normal Wistar rats, and of splenic T cells from a thyroiditis prone line of BB/W rats, when cultured in the presence of ...
Schmitt T L - - 1995
The Alzheimer beta-amyloid precursor protein (APP) is a transmembrane glycoprotein from which the amyloid beta-protein is proteolytically derived. The latter is a hydrophobic peptide that can aggregate and forms the core of the senile plaques found in the brains of patients suffering from Alzheimer's disease (AD). In view of the ...
Tischler A S - - 1995
Chronic administration of reserpine is associated with the development of pheochromocytomas in rats. Short-term administration of reserpine to rats has been shown to stimulate chromaffin cell proliferation, leading to the hypothesis that reserpine causes pheochromocytomas indirectly by providing a proliferative backdrop on which genetic damage may occur. However, it is ...
Morimoto Y - - 1995
Morphological observations of sweat glands showed degenerated debris of secretory cells in the secretory lumen in both apocrine and eccrine sweat glands. This suggested that dead secretory cells of human eccrine and apocrine sweat glands were released into the lumen and replaced by other cells. However, we did not know ...
Okada H - - 1995
The number and percentage of C-cells per unit area were investigated in 2-3-year-old sheep by an immunoperoxidase technique, using a digital-image analysis. C-cells were distributed throughout the thyroid lobes but were not present in either the isthmus or the superior and inferior poles of the thyroid. C-cells were more concentrated ...
Wang J M - - 1995
We previously demonstrated that angiotensin II (AII) is internalized in primary cultures of bovine adrenal medullary cells by receptor-mediated endocytosis. In the present work, we followed internalized AII in these cells by means of confocal laser scanning microscopy combined with analytical subcellular fractionation techniques and compared its fate with that ...
Ben Romdhane K - - 1995
A case of melanin-producing medullary thyroid carcinoma is reported in a 51-year-old man. Histologically, the tumour had a typical pattern of medullary thyroid carcinoma with numerous scattered pigmented cells which contained large amounts of melanin pigment as confirmed by bleached Fontana-Masson stain. Immunohistochemical staining revealed positivity of almost all tumour ...
Man Y G - - 1995
In the perinatal submandibular gland (SMG) of the rat, Type I cells secrete protein C (89 KD) and Type III cells secrete B1-immunoreactive proteins (20-30 KD); both cell types secrete protein D (175 KD). After the disappearance of both perinatal cell types from the maturing acini, only cells of the ...
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