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Eweiss Ahmed Z AZ Department of Otolaryngology, Derriford Hospital, Plymouth, UK ; Department of Otolaryngology, Faculty of Medicine, University of Alexandria, - - 2013
Background. The frontal recess area represents a challenge to ENT surgeons due to its narrow confines and variable anatomy. Several types of cells have been described in this area. The agger nasi cells are the most constant ones. The frontal cells, originally classified by Kuhn into 4 types, have been ...
Thanaviratananich Sanguansak - - 2012
To study the pattern of the nasofrontal drainage in relation to the neighboring anatomical structures in adult Thai cadavers. The anterior aspect of the frontal bone of the embalmed half-head was drilled to identify the presence of the frontal sinus i.e. 40 from only one side of each cadaver were ...
Otero-Gonzalez Lila - - 2012
Nanomaterials are increasingly used in a variety of industrial processes and consumer products. There are growing concerns about the potential impacts for the health and environment of engineered nanoparticles. The aim of this work was to evaluate a novel impedance-based real time cell analyzer (RTCA) as a high throughput method ...
Park Jungwon Genevieve - - 2012
Zinc (Zn2+) homeostasis plays a vital role in cell function, and the dysregulation of intracellular Zn2+ is associated with mitochondrial dysfunction. Few tools exist to quantitatively monitor the buffered, free Zn2+ concentration in mitochondria of living cells ([Zn2+]mito). We have validated three high dynamic range, ratiometric, genetically encoded, fluorescent Zn2+ ...
Sharmoukh W - - 2012
We report the synthesis and characterization of a high molar extinction coefficient ruthenium complex sensitizer (Ru (6,6'-(COOEt)(2)-2,2'-bpy)(2)(Cl)(2)). In conjugation with TiO(2) nanotube arrays as a photoactive material and iodine/iodate redox electrolyte, we fabricated efficient dye-sensitized solar cell device showing a conversion efficiency of 3.94% measured under the air mass 1.5 ...
Fan Biao B Department of Bioengineering and Therapeutic Sciences, UCSF, San Francisco, CA, - - 2012
Intrahepatic cholangiocarcinomas (ICCs) are primary liver tumors with a poor prognosis. The development of effective therapies has been hampered by a limited understanding of the biology of ICCs. Although ICCs exhibit heterogeneity in location, histology, and marker expression, they are currently thought to derive invariably from the cells lining the ...
Guo Chun Xian - - 2012
It is always challenging to construct a smart functional nanostructure with specific physico-chemical properties to real time detect bio-interesting molecules released from live-cells. We report here a new approach to build a free-standing biomimetic sensor by covalently bonding RGD-peptide on surface of pyrenebutyric acid functionalized graphene film. The resulted graphene ...
Riordon Jason - - 2012
We report the fabrication and validation of a microfluidic cell volume sensor integrated on a multi-layered polydimethylsiloxane (PDMS) microchip with a tunable detection volume for dynamic control of sensitivity, enabling the detection of individual Escherichia coli and microparticles.
Hinnebusch Alan G - - 2012
In this issue of Molecular Cell, Yanagiya et al. (2012) describe a regulatory mechanism that couples the abundance of the translational repressor 4E-BP1 with its target eIF4E via proteasomal degradation of 4E-BP1, thus maintaining translation in cells depleted of eIF4E.
Matis Maja - - 2012
Polarization of tissue is achieved by asymmetric distribution of proteins and organelles within individual cells. However, existing quantitative assays to measure this asymmetry in an automated and unbiased manner suffer from significant limitations. Here, we report a new way to assess protein and organelle localization in tissue based on correlative ...
Wilson Sandra L SL Department of Biology, Stanford University, Stanford, California 94305, - - 2012
During neural development patterning, neurogenesis, and overall growth are highly regulated and coordinated between different brain regions. Here, we show that primary cilia and the regulation of Gli activity are necessary for the normal expansion of the cerebral cortex. We show that loss of Kif3a, an important functional component of ...
Morita Shin-Ya - - 2012
Sphingomyelin (SM) is the most abundant sphingolipid in mammalian cell membranes and plays multiple physiological roles. In this study, we improved the sensitivity of the enzymatic measurement of SM and validated its specificity and accuracy. The enzymatic reaction sequence of the method involves the hydrolysis of SM by sphingomyelinase, dephosphorylation ...
Chihara Daisuke - - 2012
Gastrulation movements place endodermal precursors, mesodermal precursors and primordial germ cells (PGCs) into the interior of the embryo. Somatic cell gastrulation movements are regulated by transcription factors that also control cell fate, coupling cell identity and position. By contrast, PGCs in many species are transcriptionally quiescent, suggesting that they might ...
Ball Evan R - - 2012
The interstitial cells of Cajal (ICCs) are important mediators of gastrointestinal (GI) motility because of their role as pacemakers in the GI tract. In addition to their function, ICCs are also structurally distinct cells most easily identified by their ultra-structural features and expression of the tyrosine kinase receptor c-KIT. ICCs ...
Bonnet Jerome - - 2012
The use of synthetic biological systems in research, healthcare, and manufacturing often requires autonomous history-dependent behavior and therefore some form of engineered biological memory. For example, the study or reprogramming of aging, cancer, or development would benefit from genetically encoded counters capable of recording up to several hundred cell division ...
Bussu Francesco - - 2012
The prognosis for laryngeal squamous cell carcinoma (LSCC) has not shown any improvement in the last 30 years because of inadequate prognostic stratification. Therefore, the detection of reliable molecular markers may have a significant impact on clinical practice. As promising data regarding HER1/EGFR have been published, the purpose of the ...
Moro Enrico E Department of Biomedical Sciences, University of Padova, I-35121 Padova, Italy. - - 2012
The creation of molecular tools able to unravel in vivo spatiotemporal activation of specific cell signaling events during cell migration, differentiation and morphogenesis is of great relevance to developmental cell biology. Here, we describe the generation, validation and applications of two transgenic reporter lines for Wnt/β-catenin signaling, named TCFsiam, and ...
Korchagina Elena - - 2012
Synthetic glycolipid-like constructs dispersible in biological media and capable of incorporating into cell membranes have the ability to create novel artificial glyco-landscapes on living cells. Using a variety of different glycans ranging from disaccharides to polysaccharides, together with different lengths and high hydrophilicity spacers, we created a series of synthetic ...
Um Eujin - - 2012
We constructed a mesh-grid integrated microwell array which enables easy trapping and consistent addition of droplets. The grid acts as a microchannel structure to guide droplets into the microwells underneath, and also provides open access for additional manipulation in a high-throughput manner. Each droplet in the array forms a stable ...
Beillard Emmanuel - - 2012
MicroRNA-mRNA interactions are commonly validated and deconstructed in cell lines transfected with luciferase reporters. However, due to cell type-specific variations in microRNA or RNA-binding protein abundance, such assays may not reliably reflect microRNA activity in other cell types that are less easily transfected. In order to measure miRNA activity in ...
Polak Sebastian - - 2012
Electrical phenomena located within the plasma membrane of the mammalian cardiac cells are connected with the cells' main physiological functions--signals processing and contractility. They were extensively studied and described mathematically in so-called Hodgkin-Huxley paradigm. One of the physiological parameters, namely cell electric capacitance, has not been analyzed in-depth. The aim ...
Huang Han-Hung - - 2012
Islet equivalent (IE), the standard estimate of isolated islet volume, is an essential measure to determine the amount of transplanted islet tissue in the clinic and is used in research laboratories to normalize results, yet it is based on the false assumption that all islets are spherical. Here, we developed ...
Park E D - - 2012
In dogs, there are several idiopathic meningoencephalitides, such as necrotizing meningoencephalitis (NME), necrotizing leukoencephalitis (NLE), and granulomatous meningoencephalomyelitis (GME). Although they are often assumed to be immune mediated, the etiology of these diseases remains elusive. In this study, the histopathology of the lesions caused by these conditions and the inflammatory ...
Shkilnyy Andriy - - 2012
Scaffolds with adequate mass transport properties are needed in many tissue engineering applications. Fibrin is considered a good biological material to fabricate such scaffolds. However, very little is known about mass transport in fibrin. Therefore, a method based on the analysis of fluorescence intensity for measuring the apparent diffusion coefficient ...
Park Yang-Nim - - 2012
Diffusion coefficients of huntingtin (Htt) fragments and SOD1 mutants expressed in cells were measured using fluorescence correlation spectroscopy. The diffusion mobilities of both non-pathological Htt fragments (25 polyQs) and pathological Htt fragments (103 polyQs) were much slower than expected for monomers suggesting that they oligomerize. The mobility of these fragments ...
Martínez-Cerdeño Verónica V Department of Pathology and Laboratory Medicine, School of Medicine, University of California Davis, Sacramento, California, United States of America. - - 2012
The mammalian cerebral cortex arises from precursor cells that reside in a proliferative region surrounding the lateral ventricles of the developing brain. Recent work has shown that precursor cells in the subventricular zone (SVZ) provide a major contribution to prenatal cortical neurogenesis, and that the SVZ is significantly thicker in ...
Zhou Z L - - 2012
The elastic modulus of an oral cancer cell line UM1 is investigated by nanoindentation in an atomic force microscope with a flat-ended tip. The commonly used Hertzian method gives apparent elastic modulus which increases with the loading rate, indicating strong effects of viscoelasticity. On the contrary, a rate-jump method developed ...
Roubinet Chantal - - 2011
The cortical mechanisms that drive the series of mitotic cell shape transformations remain elusive. In this paper, we identify two novel networks that collectively control the dynamic reorganization of the mitotic cortex. We demonstrate that Moesin, an actin/membrane linker, integrates these two networks to synergize the cortical forces that drive ...
Eikmans Michael - - 2011
Microchimerism refers to the presence of less than 1% of non-host cells in a person. Our group developed a reliable method for separating viable microchimeric cells from the host environment. Optimal separation of microchimeric cells at proportions as low as 0.01% could be established with two monoclonal antibodies directed against ...
Connell Marisa M Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, - - 2011
The cytokinetic cleavage furrow is typically positioned symmetrically relative to the cortical cell boundaries, but it can also be asymmetric. The mechanisms that control furrow site specification have been intensively studied, but how polar cortex movements influence ultimate furrow position remains poorly understood. We measured the position of the apical ...
Tassan Jean-Pierre - - 2011
MELK has been implicated in a large variety of functions. Because its level is elevated in cancer tissues and it is involved in cell proliferation, MELK is considered as a potential therapeutic target for cancers. In a recent, study we have shown that MELK is involved in cytokinesis in early ...
Mashayekhi Farhad - - 2011
Hepatocyte growth factor (HGF) and its receptor, c-Met, are widely expressed in the developing brain. HGF also known as scatter factor enhances cell proliferation and cell growth, and stimulates cell migration and motility. Neurons and glia produced in the neuroepithelium migrate along radial glial fibers into the cortical plate. Reelin, ...
Zhang Zhongyan - - 2011
Previous studies using in vitro cell culture systems have shown the role of the dynamin-related GTPase Opa1 in apoptosis prevention and mitochondrial DNA (mtDNA) maintenance. However, it remains to be tested whether these functions of Opa1 are physiologically important in vivo in mammals. Here, using the Cre-loxP system, we deleted ...
Kurita Yoshihisa - - 2011
Gastropods are characterized by their asymmetric bodyplan, which develops through a unique ontogenetic process called 'torsion'. Despite several intensive studies, the driving force of torsion remains to be determined. Although torsion was traditionally believed to be driven by contraction of the retractor muscle connecting the foot and the shell, some ...
Steindl-Kuscher Kerstin - - 2011
To analyze whether epidermal growth factor (EGF) exerts regulatory effects on proliferation and differentiation in ARPE19 cells after different incubation periods (24 vs. 48 h) for obtaining ideal conditions for feasible rejuvenation and autologous transplantation of retinal pigment epithelial cells (RPE cells). To evaluate gene expression patterns of RPE-specific differentiation ...
Liang Chang-Min CM Graduate Institute of Medical Science, National Defense Medical Center, Taipei, - - 2011
To explore the effect of glucosamine (GlcN) on transforming growth factor (TGF)-β signalling and several processes involved in proliferative vitreoretinopathy (PVR). We evaluated the surface levels of TGF-β receptor and its binding of TGF-β in ARPE-19 cells. Release of cytokines and collagen, and expression of signalling intermediates were quantified. Migration ...
Zhang Yanhong - - 2011
Mutant p53 is not only deficient in tumor suppression but also acquires additional activity, called gain of function. Mutant p53 gain of function is recapitulated in knock-in mice that carry one null allele and one mutant allele of the p53 gene. These knock-in mice develop aggressive tumors compared with p53-null ...
Dogar Afzal M - - 2011
Cancer cells secreting excess latent TGF-β are often resistant to TGF-β induced growth inhibition. We observed that RNAi against TGF-β1 led to apoptotic death in such cell lines with features that were, paradoxically, reminiscent of TGF-β signaling activity and that included transiently enhanced SMAD2 and AKT phosphorylation. A comprehensive search ...
Choi Dong-Hee DH Neurology/Neuroscience Department, Weill Medical College of Cornell University, New York, New York, - - 2011
Oxidative stress is commonly implicated in aging and neurodegenerative conditions such as Parkinson's disease (PD). Mutations in DJ-1 are associated with autosomal recessive early-onset PD. We investigated whether DJ-1 can be degraded in oxidative-stressed dopaminergic neuronal cells, leading to loss of its protective role against oxidative stress. We have shown ...
Li Qin - - 2011
We previously reported that fasudil mesylate (FM) improves neurological deficit and neuronal damage in rats with ischemia following middle cerebral artery occlusion and reperfusion in vivo. In this study, the properties of FM on hydrogen peroxide (H(2)O(2))-induced oxidative stress insult in cultured PC12 cells as well as the underlying mechanisms ...
Galli C - - 2011
Little is known about how surface topography can modulate mesenchymal cell responses to oxygen-related stress occurring with age, or during the early phases of wound healing or inflammation. To antagonize Reactive Oxygen Species (ROS), cells resort to defense mechanisms, relying on β-catenin, a molecular switch between a TCF-mediated pathway, which ...
Onitsuka Takamitsu - - 2011
Positive expression of estrogen receptor (ER) beta is correlated with a favorable prognosis for patients with epidermal growth factor receptor (EGFR) mutations and predicts a good clinical outcome for patients with lung cancer after treatment with an EGFR-tyrosine kinase inhibitor (TKI), suggesting that it may be a candidate surrogate marker. ...
Lu Yong Y Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong SAR, PR - - 2011
The transforming growth factor β1 (TGF-β1) belongs to a family of structurally related polypeptide factors. TGF-beta plays an important role in the pathobiology of invasion of malignant gliomas. The objective of the present study was to investigate the impact of TNF-α converting enzyme (TACE/ADAM17) signaling on the process of TGF-β1-stimulated ...
Manesso Erica E Dept. of Information Engineering, Univ. of Padova, Via Gradenigo 6 B, 35129 Padova, - - 2011
Since the fundamental defect in both type 1 and type 2 diabetes is β-cell failure, there is increasing interest in the capacity, if any, for β-cell regeneration. Insights into typical β-cell age and lifespan during normal development and how these are influenced in diabetes is desirable to realistically establish the ...
Gennari Lorenzo - - 2011
Introduction - Beta vulgaris var. cicla (BV) leaves contain chemopreventive compounds that have been investigated for new drug discovery. These compounds belong to the family of the apigenin-glycosides. Since the leaves are seasonal products containing high percentages of water, they are easily degradable during storage in fresh conditions. To be ...
Kolegraff Keli - - 2011
Desmocollin-2 (Dsc2) and desmoglein-2 (Dsg2) are transmembrane cell adhesion proteins of desmosomes. Reduced expression of Dsc2 has been reported in colorectal carcinomas, suggesting that Dsc2 may play a role in the development and/or progression of colorectal cancer. However, no studies have examined the mechanistic contribution of Dsc2 deficiency to tumorigenesis. ...
Wang Haiyan - - 2011
Human beta-defensin-3 (HBD-3) has versatile antibacterial activity against oral bacteria and can promote the proliferation of fibroblasts. The goal of the present study was to investigate the effect of HBD-3 on attachment and proliferation of periodontal ligament cells (PDL) onto the periodontitis affected root surfaces. PDL cells were seeded onto ...
Vidro-Kotchan Eileen E The University of Texas at San Antonio, San Antonio, Texas 78249, - - 2011
Acrolein, a toxic, reactive aldehyde formed metabolically and environmentally, has been implicated in the damage to and dysfunction of the retinal pigment epithelium (RPE) that accompanies age-related macular degeneration (AMD). Our purpose was to investigate the potential of acrolein to influence the release of transforming growth factor beta-2 (TGFβ2) and ...
López-Posadas Rocío - - 2011
Intestinal inflammation produces an induction of alkaline phosphatase (AP) activity that is attributable in part to augmented expression, accompanied by a change in isoform, in epithelial cells. This study focuses on induction of AP in intestinal epithelial cells in vitro. Treatment with the oxidants H2O2, monochloramine, or tButOOH increases AP ...
Doi Shigehiro S Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, - - 2011
Fibrosis is a pathological process characterized by infiltration and proliferation of mesenchymal cells in interstitial space. A substantial portion of these cells is derived from residing non-epithelial and/or epithelial cells that have acquired the ability to migrate and proliferate. The mesenchymal transition is also observed in cancer cells to confer ...
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