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Jin Huiqin H Zhou Pei-Yuan Center for Applied Mathematics, MOE Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, - - 2014
In this paper, we study the role of autophagy in yeast cell population dynamics in response to starvation by a mathematical model based on the logistic growth model. We analytically study the boundedness of solutions, and the existence and stability of equilibrium states under general biologically acceptable assumptions. Finally, we ...
Schaum C Elisa CE Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratories, West Mains Road, Edinburgh EH9 3JF, UK - - 2014
Under global change, populations have four possible responses: 'migrate, acclimate, adapt or die' (Gienapp et al. 2008 Climate change and evolution: disentangling environmental and genetic response. Mol. Ecol. 17, 167-178. (doi:10.1111/j.1365-294X.2007.03413.x)). The challenge is to predict how much migration, acclimatization or adaptation populations are capable of. We have previously shown ...
Ugalde Unai U Department of Applied Chemistry, Faculty of Chemistry, University of the Basque Country, Manuel Lardizabal Ibilbidea, 3 20018, Donostia-San Sebastian, Spain, - - 2014
The mycelium is an organised cellular network that develops according to a functionally coherent plan. As it expands, the mycelium is capable of modulating the relative abundance of different cell types to suit the prevailing environmental conditions. This versatile pattern of multicellular development involves sophisticated environmental sensing and intercellular communication ...
El Cheikh R R CNRS UMR 5208, Institut Camille Jordan, Université Lyon 1, 43 blvd. du 11 novembre 1918, F-69622 Villeurbanne cedex, France; DRACULA Inria Grenoble Rhône-Alpes, Montbonnot F-38322, - - 2014
The circadian clock and the cell cycle are two tightly coupled oscillators. Recent analytical studies have shown counter-intuitive effects of circadian gating of the cell cycle on growth rates of proliferating cells which cannot be explained by a molecular model or a population model alone. In this work, we present ...
Klauzinska Malgorzata M Mouse Cancer Genetics Program, Center for Cancer Research, Frederick, MD 21702, - - 2014
Cripto-1 (CR-1)/Teratocarcinoma-derived growth factor1 (TDGF-1) is a cell surface glycosylphosphatidylinositol (GPI)-linked glycoprotein that can function either in cis (autocrine) or in trans (paracrine). The cell membrane cis form is found in lipid rafts and endosomes while the trans acting form lacking the GPI anchor is soluble. As a member of ...
Defaye Arnaud A Technologies Avancées pour le Génome et la Clinique (TAGC), UMR 1090 INSERM, CNRS-France, Aix Marseille Université, Parc Scientifique de Luminy, Case 928, 163 Avenue de Luminy, 13288, Marseille cedex 09, - - 2014
The Hox family of transcription factors defines cell identity along the A/P axis of animal body plan by modulating expression of distinct sets of target genes in a tissue specific manner. Identifying such tissue specific target genes is indispensable if one wants to understand how Hox proteins mediate their context ...
Liu Junli J School of Biological and Biomedical Sciences, Durham University Durham, - - 2014
Hydrodynamics, cell wall and ion dynamics are all important properties that regulate pollen tube growth. Currently, the two main pollen tube growth models, the cell wall model and the hydrodynamic model do not appear to be reconcilable. Here we develop an integrative model for pollen tube growth and show that ...
Westhorpe Frederick G - - 2012
The composition and structure of centromeric nucleosomes, which contain the histone H3 variant CENP-A, is intensely debated. Two independent studies in this issue, in yeast and human cells, now suggest that CENP-A nucleosomes adopt different structures depending on the stage of the cell cycle.
Wieland Markus - - 2012
In most approaches, personalized medicine requires time- and cost-intensive characterization of an individual's genetic background in order to achieve the best-adapted therapy. For this purpose, cell-based drug delivery offers a promising alternative. In particular, synthetic biology has introduced the vision of cells being programmable therapeutic production facilities that can be ...
Holmberg Johan - - 2012
Various studies have demonstrated that somatic differentiated cells can be reprogrammed into other differentiated states or into pluripotency, thus showing that the differentiated cellular state is not irreversible. These findings have generated intense interest in the process of reprogramming and in mechanisms that govern the pluripotent state. However, the realization ...
Anderson Kimberly L - - 2012
Viable methanogens have been detected in dry, aerobic environments such as dry reservoir sediment, dry rice paddies and aerobic desert soils, which suggests that methanogens have mechanisms for long-term survival in a desiccated state. In this study, we quantified the survival rates of the methanogenic archaeon Methanosarcina barkeri after desiccation ...
Shiomi Daisuke - - 2011
Cells lacking rodZ are not only defective in cell shape, but also in cell growth at low temperatures. Cold-sensitive growth was suppressed by a mutation of ispA without recovery from the round shape, and the mutation improved cell growth of the wild-type at low temperature.
Navarta L G LG Area de Tecnología Química y Biotecnología, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, - - 2011
The aim of the present study was to evaluate and compare freezing and freeze-drying treatments for conserving Rahnella aquatilis (BNM 0523) with the goal to achieve an adequate commercial formulation of this biocontrol agent. The effect of several protective agents, rehydration media and freezing temperatures on the viability and functional ...
Begum Shahanara - - 2011
The cold stability of microtubules during seasons of active and dormant cambium was analyzed in the conifers Abies firma, Abies sachalinensis and Larix leptolepis by immunofluorescence microscopy. Samples were fixed at room temperature and at a low temperature of 2-3°C to examine the effects of low temperature on the stability ...
Mizuno Nobuyuki - - 2011
Common wheat is an allohexaploid species originating from a naturally occurring interspecific cross between tetraploid wheat and the diploid wild wheat Aegilops tauschii Coss. Artificial allopolyploidization can produce synthetic hexaploid wheat. However, synthetic triploid hybrids can show four types of hybrid growth abnormalities: types II and III hybrid necrosis, hybrid ...
Ernst Joachim F - - 2011
In fungi, the cell wall is a scaffold, an armor and an environmental gate. Sugar polymers including protein-O- or N-linked glycosyl chains or polysaccharides such as glucan or chitin are essential components to maintain cell wall functions. We describe mechanisms in the human fungal pathogen Candida albicans, by which the ...
Whitaker Ragnhild D - - 2011
Bacterial communication via quorum sensing has been extensively investigated in recent years. Bacteria communicate in a complex manner through the production, release, and reception of diffusible low molecular weight chemical signaling molecules. Much work has focused on understanding the basic mechanisms of quorum sensing. As more and more bacteria grow ...
Lynch Andrew L - - 2011
Significant interest exists in the application of trehalose, which has low permeability to the phospholipid bilayer, as a non-toxic intracellular cryopreservative for mammalian cells. Introduction of between 8±3mM and 266±22mM trehalose into human erythrocytes using the membrane permeabilizing polymer PP-50 allowed investigation of the relationship between intracellular trehalose concentration, pre-freeze ...
Yi Song - - 2011
Like MTL-heterozygous (a/α) cells, white MTL-homozygous (a/a or α/α) cells of Candida albicans, to which a minority of opaque cells of opposite mating type have been added, form thick, robust biofilms. The latter biofilms are uniquely stimulated by the pheromone released by opaque cells and are regulated by the mitogen-activated ...
Lorentzen Anna A Institute of Cancer Research, Cancer Research UK, Cancer Research UK Tumour Cell Signalling Unit, 237 Fulham Road, London SW3 6JB, - - 2011
Melanoma cells can switch between an elongated mesenchymal-type and a rounded amoeboid-type migration mode. The rounded 'amoeboid' form of cell movement is driven by actomyosin contractility resulting in membrane blebbing. Unlike elongated A375 melanoma cells, rounded A375 cells do not display any obvious morphological front-back polarisation, although polarisation is thought ...
Bai Seoung-Jai - - 2011
The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, ...
Li Ming G - - 2011
Biofabrication that incorporates living cells to manufacture various bioproducts is often carried out at different temperatures as the process demands. In the process, cells are subjected to mechanical forces, which may damage cells if the forces reach a certain level. Previous studies have shown that the cell damage is mainly ...
Zanni Karen L - - 2011
Aim: The purpose of this paper is to review the techniques and implications of laser capture microdissection (LCM) to isolate tissue and DNA of interest using breast biopsy tissue as an example. Background: Tissues are a heterogeneous mix of different cell types, and molecular alterations are often specific to a ...
Han Yoo Jeong - - 2011
Pseudogenes are considered nonfunctional genomic artifacts of catastrophic pathways. Recent evidence, however, indicates novel roles for pseudogenes as regulators of gene expression. We tested the functionality of myosin light chain kinase pseudogene (MYLKP1) in human cells and tissues by RT-PCR, promoter activity, and cell proliferation assays. MYLKP1 is partially duplicated ...
Shao Wei - - 2011
Hierarchical anatase TiO(2) nanoplates with tunable shell structure were developed as the novel planar scattering layer in dye-sensitized solar cells, showing improved cell performance due to the enhanced light harvesting capability.
Moal Vanessa Liévin-Le - - 2011
The Secreted autotransporter toxin, Sat, which belongs to the subfamily of serine protease autotransporters of Enterobacteriaceae (SPATEs), acts as a virulence factor in extraintestinal and intestinal pathogenic strains of Escherichia coli. We observed that HeLa cells exposed to the cell-free culture supernatant of recombinant strain AAEC185(Sat) containing the Sat toxin ...
Plodinec Marija - - 2011
The contribution of the intermediate filament (IF) network to the mechanical response of cells has so far received little attention, possibly because the assembly and regulation of IFs are not as well understood as that of the actin cytoskeleton or of microtubules. The mechanical role of IFs has been mostly ...
Ettema Thijs J G - - 2011
In eukaryotic and bacterial cells, spatial organization is dependent upon cytoskeletal filaments. Actin is a main eukaryotic cytoskeletal element, involved in key processes such as cell shape determination, mechanical force generation and cytokinesis. We describe an archaeal cytoskeleton which forms helical structures within Pyrobaculum calidifontis cells, as shown by in ...
Grabinski Christin - - 2011
Gold Nanorods (GNR) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG) 5000 or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell ...
Ohbayashi Iwai - - 2011
A temperature-sensitive mutant of Arabidopsis, root initiation defective 2-1 (rid2-1), is characterized by peculiar defects in callus formation. To gain insights into the requirements for the reactivation of cell division, we analyzed this mutant and isolated the gene responsible, RID2. The phenotypes of rid2-1 in tissue culture and in seedlings ...
Tan Wenjuan - - 2011
A novel diarylethene-based iridium(III) complex was synthesized as a phosphorescence probe for monitoring living cells. The switchable phosphorescence complex in solution and within living cells was controlled by two distinguishable visible-light irradiations, which suggests that this complex can be developed as a promising probe with weak photodamage for biological samples.
Pan Yong-Le - - 2011
We incorporate optics and an ICCD to record the two-dimensional angular optical scattering (TAOS) patterns retrieved from single aerosolized cells. We analyze these patterns by performing autocorrelations and demonstrate that we are able to retrieve cell size from the locations of the secondary maxima. Additional morphological information is contained in ...
Amaro Francisco - - 2011
Heavy metals are among the most serious pollutants, and thus there is a need to develop sensitive and rapid biomonitoring methods for heavy metals in the environment. Critical parameters such as bioavailability, toxicity and genotoxicity cannot be tested using chemical analysis, but only can be assayed using living cells. A ...
Jeong Ki Jun - - 2011
Previously, we isolated the M18 scFv, which is an affinity matured antibody against the anthrax toxin PA, and observed that its single chain antibody (scAb) form (M18 scAb) exhibited superior stability compared to the scFv. Here, we report high cell density cultivations for preparative scale production of M18 scAb in ...
Selander Erik - - 2011
Chain formation is common among phytoplankton organisms but the underlying reasons and consequences are poorly understood. Here we show that chain formation is strongly impaired by waterborne cues from copepod grazers in the dinoflagellate Alexandrium tamarense. Chains of Alexandrium cells exposed to copepod cues responded by splitting into single cells ...
Lambert Guillaume - - 2011
Bacterial cells evolved under prolonged stress often have a growth advantage in stationary phase (GASP); we expect GASP cells to maintain a proliferative state and dominate wild-type cells during starvation, especially when nutrients are limited and the medium has been conditioned. However, when we compete GASP mutants against wild-type cells ...
Nawashiro Hiroshi - - 2011
To the Editor: Krueger et al. (Nov. 4 issue)(1) report on a striking effect of everolimus, an oral inhibitor of the mammalian target of rapamycin (mTOR), on subependymal giant-cell astrocytomas, which are benign tumors composed of large ganglionic astrocytes. These tumors show strong expression of CD98 (the heavy chain of ...
Jia Ji - - 2011
To the Editor: Krueger et al. (Nov. 4 issue)(1) report on a striking effect of everolimus, an oral inhibitor of the mammalian target of rapamycin (mTOR), on subependymal giant-cell astrocytomas, which are benign tumors composed of large ganglionic astrocytes. These tumors show strong expression of CD98 (the heavy chain of ...
- - 2011
To the Editor: Krueger et al. (Nov. 4 issue)(1) report on a striking effect of everolimus, an oral inhibitor of the mammalian target of rapamycin (mTOR), on subependymal giant-cell astrocytomas, which are benign tumors composed of large ganglionic astrocytes. These tumors show strong expression of CD98 (the heavy chain of ...
Wüstner Daniel - - 2011
Cholesterol with BODIPY at carbon-24 of the side chain (BCh2) has recently been introduced as new cholesterol probe with superior fluorescence properties. We compare BCh2 with the intrinsically fluorescent dehydroergosterol (DHE), a well-established marker for cholesterol, by introducing simultaneous imaging of both sterols in model membranes and living cells. BCh2 ...
Jeon Semina - - 2011
Pyridinium iodide salts, which are competitive to the conventional imidazolium iodide salts, have been used for dye-sensitized solar cells as iodide sources and ionic conductivities. Pyridinium iodide series are easy to prepare and less expensive than the imidazolium series salts. In this research, quite comparable efficiencies were obtained from electrolytes ...
Serebrovskaya Ekaterina O - - 2011
Proteins of the Green Fluorescent Protein family are widely used as passive reporters for live cell imaging. Here we used tandem phototoxic fluorescent protein KillerRed fused to core histone H2B (H2B-tKR) as an active tool to affect cell division with light. We demonstrated that H2B-tKR-expressing cells behave normally in the ...
Horie Kyoji - - 2011
A method for random relatively unbiased mutagenesis of ES cells with a mutagenic retroviral vector is described. An orderly assembly of mutant ES cells in multi-well plates is generated. 3D pooling of the wells of the assembly allows quick PCR search for insertions in genes of interest. Mutant ES cell ...
Song Yan - - 2011
Syndecan-4 is a cell membrane heparan sulfate proteoglycan that is composed of a core protein and covalently attached glycosaminoglycans (GAG) and N-linked glycosylated (N-glycosylated) chains. Syndecan-4 has been shown to function independent of its GAG chains. Syndecan-4 may derive its biological function from the N-glycosylated chains due to the biological ...
Morris Jacqueline - - 2011
Many gene expression analysis techniques rely on material isolated from heterogeneous populations of cells from tissue homogenates or cells in culture. In the case of the brain, regions such as the hippocampus contain a complex arrangement of different cell types, each with distinct mRNA profiles. The ability to harvest single ...
Yadav Jhillu S - - 2011
Organic nanomaterials having specific biological properties play important roles in in vivo delivery and clearance from the live cells. To develop orally deliverable nanomaterials for different biological applications, we have synthesized several fluorescently labelled, self-assembled PABA nanoparticles using possible acid side chain combinations and tested against insect and human cell ...
Kajiya Mikihito M Department of Immunology, Forsyth Institute, Boston, Massachusetts, United States of - - 2011
The onset and progressive pathogenesis of periodontal disease is thought to be initiated by the entry of Aggregatibacter actinomycetemcomitans (Aa) into periodontal tissue, especially gingival epithelium. Nonetheless, the mechanism underlying such bacterial entry remains to be clarified. Therefore, this study aimed to investigate the possible role of Aa outer membrane ...
Brown Michael D - - 2010
We present an investigation into incorporating core-shell Au-SiO(2) nanoparticles into dye-sensitized solar cells. We demonstrate plasmon-enhanced light absorption, photocurrent, and efficiency for both iodide/triiodide electrolyte based and solid-state dye-sensitized solar cells. Our spectroscopic investigation indicates that plasmon-enhanced photocarrier generation competes well with plasmons oscillation damping with in the first tens ...
Battaglia Corsin - - 2011
We demonstrate high-efficiency thin-film silicon solar cells with transparent nanotextured front electrodes fabricated via ultraviolet nanoimprint lithography on glass substrates. By replicating the morphology of state-of-the-art nanotextured zinc oxide front electrodes known for their exceptional light trapping properties, conversion efficiencies of up to 12.0% are achieved for micromorph tandem junction ...
Floquet Cedric F A - - 2011
We describe a novel, cost effective and simple technique for the manufacture of high sensitivity absorption cells for microfluidic analytical systems. The cells are made from tinted polymethyl methacrylate (PMMA) in which microfluidic channels are fabricated. Two windows (typically 250μm thick, resulting in little optical power loss) are formed at ...
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