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Results 301 - 350 of 1260
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Ciofani Gianni - - 2008
Boron nitride nanotubes (BNNTs) have unique physical properties, which can be exploited in the biomedical field. Hence, the surprising lack of reported studies on their biocompatibility and interactions with living cells, addressed by the present paper which deals the results of such an investigation based on 72 h culture of ...
Tsai Sheng-Fang - - 2008
Parastrombidinopsis minima n. sp. is investigated, using live observations, protargol preparations, and molecular data. In living cells, the ranges of cell length are 85-95 microm, cell width 60-70 microm, and oral diameter 40-50 microm. In protargol-impregnated specimens, cell length ranges between 43 and 71 microm, cell width between 23 and ...
Wilson C A - - 2008
Xenotransplantation is defined by the PHS as any procedure that involves the transplantation, implantation or infusion into a human recipient of either (a) live cells, tissues or organs from a nonhuman animal source, or (b) human body fluids, cells, tissues or organs that have had ex vivo contact with live ...
Harvey Tim J TJ University of Manchester, School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester, Manchester M1 7DN, United - - 2008
An investigation into the use of Raman optical tweezers to study urological cell lines is reported, with the ultimate aim of determining the presence of malignant CaP cells in urine and peripheral fluids. To this end, we trapped and analyzed live CaP cells (PC-3) and bladder cells (MGH-U1), because both ...
Jeffryes Clayton - - 2008
Diatoms are single-celled algae that make silica shells or frustules with intricate nanoscale features imbedded within periodic two-dimensional pore arrays. A two-stage photobioreactor cultivation process was used to metabolically insert titanium into the patterned biosilica of the diatom Pinnularia sp. In Stage I, diatom cells were grown up on dissolved ...
Cheng Jinping J Department of Biology and Chemistry, City University of Hong Kong, Hong - - 2008
Carbon nanotubes (CNTs) have been shown to cross cell membranes and can mediate the internalization of macromolecules. These characteristics have constituted CNTs as an exciting new tool for drug delivery and biological sensing. While CNTs exhibit great potential in biomedical and pharmaceutical applications, neither the cell penetration mechanism of CNTs ...
Lee Philip J - - 2009
We present a microfluidic system for time-lapsed, live cell microscopy with the ability to control solution exchange via a dynamic flow controller. The application specific microfluidic plates are designed to maintain adherent and non-adherent cell types for multiple days with continuous medium perfusion. Upstream channels with flow controlled via custom ...
Ersoy I - - 2008
The large amount of data produced by biological live cell imaging studies of cell behavior requires accurate automated cell segmentation algorithms for rapid, unbiased and reproducible scientific analysis. This paper presents a new approach to obtain precise boundaries of cells with complex shapes using ridge measures for initial detection and ...
Chowdhury Farhan - - 2008
A living cell deforms or flows in response to mechanical stresses. A recent report shows that dynamic mechanics of living cells depends on the timescale of mechanical loading, in contrast to the prevailing view of some authors that cell rheology is timescale-free. Yet the molecular basis that governs this timescale-dependent ...
Tang Hong-Wu - - 2008
By using near-infrared surface-enhanced Raman scattering (SERS) with 60 nm gold nanoparticles (Au-NPs) to probe the chemical composition inside single human osteosarcoma cells we have shown that the SERS intensity may increase by a factor of 3-6 times in different parts of the cells depending on the density of gold ...
Chen Bo - - 2008
Conjugation of the cell-penetrating peptide derived from the human immunodeficiency virus-1 transactivator protein (TAT) to semiconductor quantum dots (QDs) is an effective way to enhance transmembrane delivery of QDs for intracellular and molecular imaging. In this work, the internalization pathway of TAT-QDs was studied systematically in living cells. Cellular uptake ...
Yoo Jungwoo - - 2008
We developed a new method for imaging the movement of targeted proteins in living cancer cells with photostable and bright quantum dots (QDs). QDs were conjugated with various molecules and proteins, such as phalloidin, anti-tubulin antibody and kinesin. These bioconjugated QDs were mixed with a transfection reagent and successfully internalized ...
Sinha Deepak Kumar - - 2008
The three-dimensional organization of nuclear compartments within living cells determines genome function and yet their underlying self-organizing principles are unclear. We visualize in real-time transcriptionally active compartments (TCs) by the transient enrichment of fluorescently-labeled uridine 5'-triphosphate molecules within living cells. These TCs partially colocalize with active RNA-Pol II in the ...
Sabelnikov Alex - - 2008
A general problem in single-cell research (SCR) is whether a representative cell is picked for analysis. In many cases, it may determine feasibility of SCR. Here, using a probability approach, we analyze this problem for laser tweezers Raman spectroscopy (LTRS), a promising technique for SCR. The results suggest that two ...
Yu Hui - - 2009
The paper discussed a novel design of multifunctional cell-based biosensors for simultaneously detecting cell acidification and extracellular potential. Employing living cells such as cardiac myocytes as a source for the light addressable potentiometric sensor (LAPS) array, this cell-based biosensor was able to monitor both the acidification and extracellular potential in ...
Timp, Winston (Winston G.)
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.
Kunapareddy Nagapratima - - 2008
Raman spectroscopy has been used to estimate the biochemical changes due to necrosis in an in vitro model system comprised of a human malignant melanoma cell line (MEL-28). Combined oxygen and glucose deprivation was used to simulate necrotic cell death in tumors. Raman spectroscopy measurements of nonproliferating live cells and ...
Greig Duncan - - 2008
The ability to directly engineer living cells is rapidly becoming a hot field of research for a wide range of applications within the life sciences. 'Bio-electrospraying' cells, a recently developed technique, has great potential in this area. In this paper, we quantify genetic, genomic and physiological effects of bio-electrospraying cells ...
Zimberlin Jessica A - - 2008
Both individual cells and sheets of cells exert traction forces on the substrate and these forces have been investigated using a wide range of methods. Here we compare the mechanical properties of fibroblasts and epithelial cells using a novel surface geometry. Living cells are added to a thin film of ...
Chen Rong - - 2008
Although the spatial and temporal distributions of cellular NAD(P)H concentrations have been theoretically predicted as typical patterns of the metabolism in living cells, so far such a pattern was observed only in neutrophils. In this work, the dynamic NAD(P)H distributions in rat basophilic leukemia (RBL-2H3) and human hepatocellular carcinoma (Hep ...
Prakash Satya - - 2008
There has been an ample interest in delivery of therapeutic molecules using live cells. Oral delivery has been stipulated as best way to deliver live cells to humans for therapy. Colon, in particular, is a part of gastrointestinal (GI) tract that has been proposed to be an oral targeted site. ...
Chang Yun-Pei YP Department of Chemistry and Biochemistry, University of California at Los Angeles, 607 Charles Young Drive, East Los Angeles, California 90095-1569, USA. - - 2008
Single particle tracking (SPT) techniques were developed to explore bio-molecules dynamics in live cells at single molecule sensitivity and nanometer spatial resolution. Recent developments in quantum dots (Qdots) surface coating and bio-conjugation schemes have made them most suitable probes for live cell applications. Here we review recent advancements in using ...
Na Sungsoo - - 2008
Mechanotransduction is the process by which living cells sense mechanical forces and then convert them into biochemical signaling. Recently we showed that mechanical stress is transduced from the cell surface to remote cytoplasmic sites within 0.3 s, which is at least 40 to 50 times faster than soluble factor-induced signal ...
Sugimoto Kenji - - 2008
Mitotic events from prophase to telophase are defined by morphology or movement of chromatin, nuclear envelope, centrosomes and spindles. Live-cell imaging is useful for characterizing the whole chromosome segregation process in the living state. In this study, we constructed three quadruple-fluorescent MDA435 cell lines in which chromatin, kinetochores, nuclear envelope ...
Othon Christina M - - 2008
Biological laser printing (BioLP) is a unique tool capable of printing high resolution two- and three-dimensional patterns of living mammalian cells, with greater than 95% viability. These results have been extended to primary cultured olfactory ensheathing cells (OECs), harvested from adult Sprague-Dawley rats. OECs have been found to provide stimulating ...
Ankers John M JM Centre for Cell Imaging, School of Biological Sciences, The Biosciences Building, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK. - - 2008
The development and application of single cell optical imaging has identified dynamic and oscillatory signalling processes in individual cells. This requires single cell analyses since the processes may otherwise be masked by the population average. These oscillations range in timing from seconds/minutes (e.g. calcium) to minutes/hours (e.g. NF-kappaB, Notch/Wnt and ...
Graefe Anja - - 2008
In this study, we describe the preparation and evaluation of new fluorescent sensor nanoparticles for the ratiometric measurement of chloride concentrations. Both a chloride-sensitive dye (lucigenin) and a reference dye (sulforhodamine derivative) were incorporated into polyacrylamide nanoparticles via inverse microemulsion polymerization and investigated for their response to chloride ions in ...
Terryn Christine - - 2009
Live-cell imaging has become a powerful analytical tool in most cell biology laboratories. The scope of this paper is to give an overview of the environmental considerations for maintaining living cells on the microscope stage and the technical advances permitting multi-parameter imaging. The paper will then focus on two-dimensional and ...
Ryu Wonhyoung - - 2008
Ultra-sharp nano-probes and customized atomic force microscopy (AFM) have previously been developed in our laboratory for in situ sub-cellular probing of electrochemical phenomena in living plant cells during their photosynthesis. However, this AFM-based electrochemical probing still has numerous engineering challenges such as immobilization of the live cells, compatibility of the ...
Li Q S - - 2008
Mechanical properties of individual living cells are known to be closely related to the health and function of the human body. Here, atomic force microscopy (AFM) indentation using a micro-sized spherical probe was carried out to characterize the elasticity of benign (MCF-10A) and cancerous (MCF-7) human breast epithelial cells. AFM ...
Poirier C - - 2008
This paper presents a statistical experimental study of the adsorption of colloids onto the plasma membrane of living cells mediated by specific ligand-receptor interactions. The colloids consist of lipid multilamellar liposomes (spherulites) functionalized by Shiga toxin B-subunit (STxB), while cells are cervix carcinoma epithelial cells expressing the Shiga toxin receptor, ...
Horvath Robert - - 2008
The use of planar optical waveguides as substrata for label-free, non-invasive monitoring of cells growing on them is demonstrated. Different submicrometre depths (measured from and perpendicular to the substratum surface) can be selected for monitoring. The so-called symmetry waveguide configuration with a low refractive index waveguide support (nanoporous silica with ...
So Min-kyung MK Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, P093, Mail code 5484, Stanford, CA 94305, - - 2008
Quantum dots emerge as an attractive alternative to small molecule fluorophores as fluorescent tags for in vivo cell labeling and imaging. This communication presents a method for specific labeling of live cells using quantum dots. The labeling is mediated by HaloTag protein expressed at the cell surface which forms a ...
Wilhelm Claire - - 2008
Both forced and spontaneous motions of magnetic microbeads engulfed by Dictyostelium cells have served as experimental probes of intracellular dynamics. The complex shear modulus G*(omega), determined from active oscillatory measurements, has a power-law dynamics and increases with the probe size, reflecting intracellular structural complexity. The combined use of passive microrheology ...
Clift Martin J D - - 2008
This study investigated the uptake, kinetics and cellular distribution of different surface coated quantum dots (QDs) before relating this to their toxicity. J774.A1 cells were treated with organic, COOH and NH2 (PEG) surface coated QDs (40 nM). Model 20 nm and 200 nm COOH-modified coated polystyrene beads (PBs) were also ...
Swain Robin J - - 2008
Raman micro-spectroscopy is a laser-based technique which enables rapid and non-invasive biochemical analysis of cells and tissues without the need for labels, markers or stains. Previous characterization of the mammalian cell cycle using Raman micro-spectroscopy involved the analysis of suspensions of viable cells and individual fixed and/or dried cells. Cell ...
Gladilin E - - 2008
Topological analysis of cells and subcellular structures on the basis of image data, is one of the major trends in modern quantitative biology. However, due to the dynamic nature of cell biology, the optical appearance of different cells or even time-series of the same cell is undergoing substantial variations in ...
Citovsky Vitaly - - 2008
The application of novel assays for basic cell research is tightly linked to the development of easy-to-use and versatile tools and protocols for implementing such technologies for a wide range of applications and model species. The bimolecular fluorescence complementation (BiFC) assay is one such novel method for which tools and ...
Hodgson Louis - - 2008
New genetically encoded biosensors utilizing the modified firefly luciferase promise a great improvement in the signal-to-noise ratio and the dynamic range of response in living cells. These biosensors are particularly suitable for high-throughput screening assays that use large-well-capacity formats because of their excellent response characteristics. The biosensor design strategies are ...
Mangold Shane L SL Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, - - 2008
The binding of a polymeric ligand to a cell surface receptor can promote its internalization. Methods to track and visualize multivalent ligands within a cell can give rise to new therapeutic strategies and illuminate signaling processes. We have used the features of the ring-opening metathesis polymerization (ROMP) to develop a ...
Popescu Gabriel - - 2008
Using novel interferometric quantitative phase microscopy methods, we demonstrate that the surface integral of the optical phase associated with live cells is invariant to cell water content. Thus, we provide an entirely noninvasive method to measure the nonaqueous content or "dry mass" of living cells. Given the extremely high stability ...
Rappoport Joshua Z - - 2008
Investigations into the mechanisms which regulate entry of integral membrane proteins, and associated ligands, into the cell through vesicular carriers (endocytosis) have greatly benefited from the application of live-cell imaging. Several excellent recent reviews have detailed specific aspects of endocytosis, such as entry of particular cargo, or the different routes ...
Weber Darin J - - 2008
Developers of cell-based therapies intended for the treatment of cardiovascular indications must address a number of regulatory issues related to the manufacturing and delivery of living cell products to patients. This article highlights issues that must be considered regarding the cell and tissue source and ancillary materials, the development of ...
Fernández Pablo - - 2008
Cell mechanical properties are fundamental to the organism but remain poorly understood. We report a comprehensive phenomenological framework for the complex rheology of single fibroblast cells: a superposition of elastic stiffening and viscoplastic kinematic hardening. Despite the complexity of the living cell, its mechanical properties can be cast into simple, ...
Wang Hsiang-Yu - - 2008
Microfluidic arrays of living cells have raised a lot of interests recently due to their potential for high throughput screening of cell-based assays. This report presents a microfluidic cell array with individually addressable chambers controlled by pneumatic valves for cell culture and treatment. There are two modes for the cell ...
Park Kidong K Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, - - 2008
The size of a cell is a fundamental physiological property and is closely regulated by various environmental and genetic factors. Optical or confocal microscopy can be used to measure the dimensions of adherent cells, and Coulter counter or flow cytometry (forward scattering light intensity) can be used to estimate the ...
Wei Ming-Tzo - - 2008
Micromechanical properties of biological cells are crucial for cells functions. Despite extensive study by a variety of approaches, an understanding of the subject remains elusive. We conducted a comparative study of the micromechanical properties of cultured alveolar epithelial cells with an oscillatory optical tweezer-based cytorheometer. In this study, the frequency-dependent ...
Farruggia Giovanna - - 2009
The intracellular behaviour of diaza-18-crown-6 appended with two H-substituted hydroxyquinoline groups (DCHQ1) was investigated to explore its application as a new sensor for the evaluation of cell magnesium content and distribution. We used five cells lines characterised by different contents of magnesium and different intracellular membrane-defined compartments. The main result ...
Nagayama, Masafumi
We improved the force modulation mode with scanning probe microscopy (SPM) in order to make a quantitative evaluation of the viscoelasticity of living cells. Taking account of the viscosity of liquid medium, the vibration frequency of the cantilever was selected to be 500 Hz, and analysis of cantilever vibration was ...
Lin Junzhang - - 2008
Oral administration of artificial cell microcapsules entrapping live bacterial cells is a promising approach in disease therapy. However, the current technology of microcapsules limits this approach. In this study, alginate-chitosan-alginate (ACA) microcapsules entrapping live bacterial cells were prepared with the purpose of oral delivery for therapy, and their in vitro ...
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