Search Results
Results 201 - 250 of 1261
< 1 2 3 4 5 6 7 8 9 10 >
Nair Kalyani - - 2009
Bioprinting is an emerging technology in the field of tissue engineering and regenerative medicine. The process consists of simultaneous deposition of cells, biomaterial and/or growth factors under pressure through a micro-scale nozzle. Cell viability can be controlled by varying the parameters like pressure and nozzle diameter. The process itself can ...
Saxena Chaitanya - - 2009
Recently we have described the development of an Immuno-chemo-proteomics method for drug target deconvolution and profiling the toxicity of known drugs ( Saxena , C. ; Zhen , E. ; Higgs , R. E. ; Hale , J. E. J. Proteome Res. 2008, 8 , 3490 - 3497 ). The ...
Masujima Tsutomu - - 2009
The history from bio-imaging to live single-cell mass spectrometry (MS) is herein reviewed. The limitation of the current bio-imaging method is probing only known molecules, and a method for finding new molecules is needed for cells which, however, show individual behaviors even in the same incubation dish. Single-cell MALDI-TOF/MS has ...
Izumi Saki - - 2009
Noninvasive visualization and investigation of interactions among proteins, biomolecules, and enzymes in living cells is an important goal for biologists, and fluorescence probes are powerful tools for this purpose. Because many target molecules are present in only trace amounts, high sensitivity is very important, and it is common to improve ...
Oruganti Prasad - - 2009
The introduction of specific molecules into live cells is a widely used approach to probe cellular mechanisms. Recently, we have reported on the sustained dosing of molecules into single cells via a microscopic diffusion port. Here we describe temporal ratiometry, a method to reconstruct intracellular concentration distribution of the delivered ...
Wang Longxiang - - 2009
Confocal fluorescence imaging and fluorescence resonance energy transfer (FRET) technology have been widely used to study protein-protein interactions in living cells. However, it is very difficult to quantitatively analyze FRET efficiency due to the excitation spectral crosstalk and emission spectral crosstalk between donor and acceptor. In this study, we developed ...
Suissa M - - 2009
Progress in cellular biology based on fluorescent microscopy techniques, shows that the spatial organization of the nucleus is dynamic. This dynamic is very complex and involves a multitude of phenomena that occur on very different time and size scales. Using an original light scattering experimental device, we investigated the global ...
Schiffenbauer Yael S - - 2009
Advances in molecular cell biology, medical research, and drug development are driving a growing need for technologies that enable imaging the dynamics of molecular and physiological processes simultaneously in numerous non-adherent living cells. Here we describe a platform technology and software--the CKChip system--that enables continuous, fluorescence-based imaging of thousands of ...
Hajighasemi Fatemeh - - 2009
Propranolol, as a beta-adrenergic blocker is used for treatment of a large number of cardiovascular diseases such as hypertension and arrhythmias. The inhibitory effects of propranolol on tumor cells growth and also its cytotoxicity on cancerous cells have been revealed by several studies. In this study the sensitivity of a ...
Sun Y-N - - 2009
The analysis of cell motion is an essential process in fundamental medical studies because most active cellular functions involve motion. In this paper, a computer-assisted motion analysis system is proposed for cell tracking. In the proposed tracking process, unlike in conventional tracking methods, cellular states referring to the cellular life ...
Orndorff Rebecca L - - 2009
High affinity peptide neurotoxins are effective agents for integrating technological advances with biological inquiries. Both chlorotoxin (CTX) and dendrotoxin-1 (DTX-1) are peptide neurotoxins demonstrated to bind targets expressed by glioma cancer cells and are suitable ligands for quantum dot (QD) live cell investigations. Here, we present dual labeling of endogenously ...
Roberti M Julia - - 2009
Quantum dots multifunctionalized with the amyloid protein alpha-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only approximately 100 ...
Idris Niagara Muhammad - - 2009
With the emergence of cell transplant as an attractive treatment modality for various diseases, there is a parallel need to track the fate of these cells to assess their therapeutic effectiveness. Here, we report the use of upconversion fluorescent nanoparticles, silica/NaYF(4):Yb,Er, to dynamically track live myoblast cells in vitro and ...
Fakhrullin Rawil F - - 2009
We report the fabrication of hybrid cellular-inorganic core-shell microparticles obtained by encapsulation of individual living yeast cells Saccharomyces cerevisiae in calcium carbonate microshells and demonstrate the viability of the encapsulated cells. Our method is based on the direct precipitation of calcium carbonate on the cell walls of yeast cells. Resulting ...
Mazumder M A Jafar - - 2009
Self-cross-linkable polyelectrolyte pairs comprised of poly(methacrylic acid, sodium salt-co-2-[methacryloyloxy]ethyl acetoacetate) (70:30 mol ratio, A70) and poly-L-lysine are incorporated into CaAlg beads to form either a covalently cross-linked shell or a core-cross-linked bead. In both cases the reactive polyanion is added to a solution of sodium alginate that may contain live ...
Müller Daniel J - - 2009
Biological processes rely on molecular interactions that can be directly measured using force spectroscopy techniques. Here we review how atomic force microscopy can be applied to force probe surfaces of living cells to single-molecule resolution. Such probing of individual interactions can be used to map cell surface receptors, and to ...
Meister André - - 2009
We describe the fluidFM, an atomic force microscope (AFM) based on hollow cantilevers for local liquid dispensing and stimulation of single living cells under physiological conditions. A nanofluidic channel in the cantilever allows soluble molecules to be dispensed through a submicrometer aperture in the AFM tip. The sensitive AFM force ...
Kim Tae-Jin TJ Neuroscience Program University of Illinois at Urbana-Champaign, IL 61801, - - 2009
The cell-microenvironment interaction is critical for cells to perceive environmental cues and co-ordinate signaling cascades to regulate physiological functions. Herein, a soft-lithography technique, micropattern via micromolding in capillaries (MIMIC), is explored to create cell-adhesive micropatterns on glass coverslips. Genetically encoded Src and RhoA fluorescence resonance energy transfer (FRET) biosensors are ...
Pierobon Paolo P Institut Curie, Centre de Recherche, Laboratoire Physico-Chimie Curie, Centre National de la Recherche Scientifique, UMR 168, Université Pierre et Marie Curie-Paris, Paris, - - 2009
We report the tracking of single myosin V molecules in their natural environment, the cell. Myosin V molecules, labeled with quantum dots, are introduced into the cytoplasm of living HeLa cells and their motion is recorded at the single molecule level with high spatial and temporal resolution. We perform an ...
Jerrim Lindsey B LB Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695, - - 2009
Convective assembly at high volume fraction was used for the rapid deposition of uniform, close-packed coatings of Saccharomyces cerevisiae yeast cells onto glass slides. A computational model was developed to calculate the thickness profiles of such coatings for different set of conditions. Both the experiments and the numerical simulations demonstrated ...
de la Rica Roberto - - 2009
Detection of physical changes of cells is emerging as a new diagnostic approach to determine their phenotypical features. One of such changes is related to their viability; live (viable) cells are more voluminous than the dead ones, and monitoring this parameter in tissue cells becomes essential in fields such as ...
Nowotschin Sonja S Sloan-Kettering Institute, New York, New York, - - 2009
To simultaneously follow multiple subcellular characteristics, for example, cell position and cell morphology, in living specimens requires multiple subcellular labels. Toward this goal, we generated dual-tagged mouse embryonic stem (ES) cells constitutively expressing differentially localized, spectrally distinct, genetically encoded fluorescent protein fusions. We have used human histone H2B fusions to ...
Yum Kyungsuk K Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, Illinois 61801, - - 2009
Studying molecular dynamics inside living cells is a major but highly rewarding challenge in cell biology. We present a nanoscale mechanochemical method to deliver fluorescent quantum dots (QDs) into living cells, using a membrane-penetrating nanoneedle. We demonstrate the selective delivery of monodispersed QDs into the cytoplasm and the nucleus of ...
Lu Hua - - 2009
A boron-dipyrromethene (BODIPY) derivative containing a tridentate diaza-oxa ligand (8H-BDP) was synthesized as a fluorescent turn-on chemosensor for Hg(2+) with high sensitivity (detection limit < or = 2 ppb), a rapid response time (< or = 5 seconds) and specific selectivity over other cations under physiological conditions and in live ...
Fakhrullin Rawil F - - 2009
We report the layer-by-layer coating of living fungi cells (Saccharomyces cerevisiae and Trichoderma asperellum) with polyelectrolytes poly(allylamine hydrochloride)/sodium poly(styrene sulfonate) and bovine serum albumin/DNA and citrate-stabilized gold and silver nanoparticles. It was found that the nanoparticles were effectively incorporated between oppositely charged polyelectrolyte layers, modifying the topography and the roughness ...
Benenson Yaakov - - 2009
Biocomputers are man-made biological networks whose goal is to probe and control biological hosts--cells and organisms--in which they operate. Their key design features, informed by computer science and engineering, are programmability, modularity and versatility. While still a work in progress, biocomputers will eventually enable disease diagnosis and treatment with single-cell ...
Edward Roy - - 2009
Image-based, high-content screening assays demand solutions for image segmentation and cellular compartment encoding to track critical events--for example those reported by GFP fusions within mitosis, signalling pathways and protein translocations. To meet this need, a series of nuclear/cytoplasmic discriminating probes have been developed: DRAQ5 and CyTRAK Orange. These are spectrally ...
Mejean Cecile O CO Department of Mechanical Engineering, Yale University, New Haven, CT 06511, - - 2009
We describe open-loop and closed-loop multiplexed force measurements using holographic optical tweezers. We quantify the performance of our novel video-based control system in a driven suspension of colloidal particles. We demonstrate our system's abilities with the measurement of the mechanical coupling between Aplysia bag cell growth cones and beads functionalized ...
Ou Guangshuo G The Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94158, - - 2009
Metazoan cell movement has been studied extensively in vitro, but cell migration in living animals is much less well understood. In this report, we have studied the Caenorhabditis elegans Q neuroblast lineage during larval development, developing live animal imaging methods for following neuroblast migration with single cell resolution. We find ...
Raabe I - - 2009
Living cells are highly organized in space and time, which makes spatially and temporally confined manipulations an indispensable tool in cell biology. Laser-based nanosurgery is an elegant method that allows precise ablation of intracellular structures. Here, we show cutting of fluorescently labelled microtubules and mitotic spindles in fission yeast, performed ...
Shin Sang-Mo - - 2009
Time-resolved scanning acoustic microscopy (SAM) equipped with time-resolved signal analysis offers unique possibilities for studying the mechanical properties of cells in vitro. The method is nondestructive and noninvasive on a cellular level, which enables real-time measurements on living cells. The ultrasonic attenuation coefficients and sound speeds of MCF 10A normal ...
Takahashi Yasufumi - - 2009
A membrane protein on the surface of a single living mammalian cell was imaged by scanning electrochemical microscopy (SECM). The epidermal growth factor receptor (EGFR) is one of the key membrane proteins associated with cancer. It elicits a wide range of cell-type-specific responses, leading to cell proliferation, differentiation, apoptosis, and ...
Gupta Roohi - - 2009
Most interactions inside living cells take place at nano-length scales. The only way to monitor these nano-scale interactions inside living cells is by utilizing the phenomenon of Fluorescence Resonance Energy Transfer (FRET). Thus, the importance of FRET in visualizing intracellular dynamics has provided continuous motivation for discovery/synthesis of biologically useful ...
Xie Wei - - 2009
We present a novel nuclear targeting nanoprobe based on peptide functionalized gold nanoparticles and its surface-enhanced Raman scattering (SERS) in living cells. For the first time, we probe an original SERS signal from the living cell nucleus by using high-selectivity functionalized gold nanoparticles. The gold nanoparticles conjugated with SV-40 large ...
Suciu L - - 2009
During the last few years, there is an increasing interest in the role of the epicardium in cardiac development, myocardial remodelling or repair and regeneration. Several types of cells were described in the subepicardial loose connective tissue, beneath the epicardial mesothe-lium. We showed previously (repeatedly) the existence of interstitial Cajal-like ...
Hiratsuka Shinichiro - - 2009
The viscoelastic properties of a large number of mouse fibroblast NIH3T3 cells (n approximately 130) were investigated by combining atomic force microscopy (AFM) with a microarray technique. In the experiments, the cells were arranged and cultured in the wells of a microarray substrate, and a force modulation mode experiment was ...
Li Shu-Hong - - 2009
OBJECTIVES: Cell therapy improved cardiac function after a myocardial infarction in several preclinical studies; however, the functional benefits were limited in the initial clinical trials, perhaps because of inadequate cell engraftment. We used noninvasive molecular imaging to compare the distribution and myocardial retention of cells implanted by using clinical delivery ...
KarataƟ Omer Faruk - - 2009
Mitochondrion is one of the most important organelles in cells with several vital responsibilities. The consequence of a deficiency in the function of mitochondrion could result with the wide range of diseases and disorders. In this study, we investigated the feasibility of utilizing surface-enhanced Raman scattering (SERS) to understand the ...
Cai Xiaofang - - 2009
In this study, we have used atomic force microscopy (AFM) to study the morphology and mechanical property changes of Jurkat cells exposed to different concentrations of Artesunate (ART) for 24 h at single cellular level. Cell viability and proliferation assays were performed by using the Cell Counting Kit-8. The concentration ...
Matsunaga Shigeru - - 2009
Using a combination of silicone and urethane resin, we established a rapid technique for preparing living specimens for microscopy. One major advantage of this technique is that the coverslip is rigidly attached and does not detach during handling. As a result, it is possible to continuously observe living cells at ...
Watson Peter - - 2009
Cell biology has the ability to yield an incredible amount of information regarding cell activity, and is a powerful tool to both discover targets for, and examine the effects of therapeutic molecules on model systems. At the forefront of cell biology is a wide array of microscopy-based techniques that allow ...
Hao Yanhong - - 2009
Although transgenic animal production through somatic cell nuclear transfer (SCNT) has been successful, the process is still inefficient. One major limitation is the use of somatic donor cells that have a finite life span. Identification and isolation of a cell type capable of rapid proliferation while possessing immortal or prolonged ...
van de Ven Anne L AL Rice University, Department of Bioengineering, MS 142, Keck Hall, Suite 116, 6100 Main Street, Houston, Texas 77005, - - 2009
Effective delivery of optical contrast agents into live cells remains a significant challenge. We sought to determine whether Triton-X100, a detergent commonly used for membrane isolation and protein purification, could be used to effectively and reversibly permeabilize live cells for delivery of targeted optical contrast agents. Although Triton-X100 is widely ...
Machado M D - - 2009
AIM: The capacities of live and heat-killed cells of Saccharomyces cerevisiae at 45 degrees C for the removal of copper, nickel and zinc from the solution were compared. METHODS AND RESULTS: Kinetic studies have shown a maximum accumulation of Ni(2+) and Zn(2+) after 10 min for both types of cells, ...
Fujita Satoshi - - 2009
Cells elongate on a surface with nanogrooved (NG) patterns and align along that pattern. Although various models have been proposed for how this occurs, much remains to be clarified. Studies with fixed cells do not lend themselves to answering some of these open questions. In this study, the dynamic behaviours ...
Na Sungsoo S Dept. of Mechanical Science, Univ. of Illinois, Urbana, IL 61801, - - 2009
Plectin is a 500-kDa cross-linking protein that plays important roles in a number of cell functions including migration and wound healing. We set out to characterize the role of plectin in mechanical properties of living cells. Plectin(-/-) cells were less stiff than plectin(+/+) cells, but the slopes of the two ...
Zeng Ying Y Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA 92037, - - 2009
We describe a simple method for efficiently labeling cell-surface sialic acid-containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of ...
Butler Jonathan T JT Department of Molecular and Cellular Biology and Pathobiology, Medical University of South Carolina, Charleston, South Carolina 29425, - - 2009
Massive T cell infiltration into the central nervous system is a hallmark of multiple sclerosis (MS) and its rodent model experimental autoimmune encephalomyelitis (EAE), resulting in the induction of many of the pathophysiological events that lead to neuroinflammation and neurodegeneration. Thus, blocking T cell migration into the central nervous system ...
Kailas L - - 2009
Coccoid cells of the bacterial species Staphylococcus aureus have been mechanically trapped in lithographically patterned substrates and imaged under growth media using atomic force microscopy (AFM) in order to follow cellular processes. The cells are not perturbed as there is no chemical linkage to the surface. Confinement effects are minimized ...
Koo Chi-Kin - - 2009
The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C(phenyl), N(pyridyl), and N(pyrazolyl) donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 ...
< 1 2 3 4 5 6 7 8 9 10 >