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Repin V S VS Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, - - 2014
We developed an original reproducible 3D-technology for preparation of single dormant microspheres consisting of 2000 somatic cells. The dynamics of microsphere assembly from mesenchymal and epithelial cells of retinal pigment epithelium was traced using time-lapse microscopy: formation of a loose aggregate over 24 h followed by its gradual consolidation and ...
Lungova Vlasta V Division of Otolaryngology Head and Neck Surgery, Department of Surgery, University of Wisconsin Madison, Madison, WI, 53705, - - 2014
Vocal fold epithelial cells are very difficult to study as the vocal fold epithelial cell lines do not exist and they cannot be removed from the healthy larynx without engendering a significant and unacceptable risk to vocal fold function. Here, we describe the procedure to create an engineered vocal fold ...
Zhang Yu Y Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, - - 2014
Our previous studies have shown that podocyte number is significantly decreased in glomeruli of children with hepatitis B virus (HBV)-associated glomerulonephritis. In this study, we aimed to explore whether exogenous expression of HBx protein could directly inhibit podocyte proliferation in vitro, and to investigate its role in cell cycle regulation. ...
Kumari Archana A University of - - 2014
Taste sensation on the anterior tongue requires chorda tympani nerve function and connections with continuously renewing taste receptor cells. However, it is unclear which signaling pathways regulate the receptor cells to maintain chorda tympani sensation. Hedgehog (HH) signaling controls cell proliferation and differentiation in numerous tissues and is active in ...
Bellenberg Sören S Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany, - - 2014
In this study, the process of pyrite colonization and leaching by three iron-oxidizing Acidithiobacillus species was investigated by fluorescence microscopy, bacterial attachment, and leaching assays. Within the first 4-5 days, only the biofilm subpopulation was responsible for pyrite dissolution. Pyrite-grown cells, in contrast to iron-grown cells, were able to oxidize iron(II) ...
Yun Jieun J 1 Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology , Ochang, Republic of - - 2014
Abstract This study was performed to elucidate the effect of a lipid-soluble ginseng extract (LSGE) on cancer invasion and metastasis. The LSGE, even at noncytotoxic concentrations, potently inhibited invasion and migration of B16F10 mouse melanoma cells in a dose-dependent manner. In the presence of 3 μg/mL of LSGE, the invasion ...
Rauš Balind Snežana S Institute for Biological Research Siniša Stanković, University of Belgrade, Belgrade, - - 2014
The aim of the present study was to determine does extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affect pituitary adrenocorticotroph (ACTH) cells in adult animals. We performed two series of experiments: (1) short-term exposure of 3-month-old rats to ELF-MF for 1 and 7 days, and (2) long-term ...
Zhang Kexiong K Institute of Aging Research, Hangzhou Normal University School of Medicine, No.1378, Wen-Yi West Road, Hangzhou, 311121, Zhejiang Province, - - 2014
Cellular senescence protects multicellular organisms from tissue overgrowth including cancer, and contributes to tissue ageing. With stable cell cycle arrests and particular molecular features, cellular senescence has been mostly studied in the adult tissues of animals including mammals. In the present study, we report a widespread of cellular senescence in ...
Sankar Rathinam K RK Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan Pune 411 008, Maharashtra, India. - - 2014
A thiol-selective 2-methyl-3-phenacrylate scaffold with spatiotemporal control over delivery of a cargo is reported. The half-lives of decomposition could be tuned from 30 min to 1 day and the scaffold's utility in thiol-inducible fluorophore release in cell-free as well as within cells is demonstrated.
Pulikkot S S Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, United Arab - - 2014
To generate various polycaprolactone (PCL) scaffolds and test their suitability for growth and differentiation of immortalized mouse gastric stem (mGS) cells. Non-porous, microporous and three-dimensional electrospun microfibrous PCL scaffolds were prepared and characterized for culture of mGS cells. First, growth of mGS cells was compared on these different scaffolds after ...
Bernardini André A University of - - 2014
Reactive oxygen species (ROS) mainly originating from NADPH oxidases have been shown to be involved in the carotid body (CB) oxygen sensing cascade. For measuring ROS kinetics type I cells of the mouse CB in an ex vivo preparation were transfected with the ROS sensor construct FRET-HSP33. After 2 days ...
Li Ruili R Douglas Stephens Surgical Research laboratory, Murdoch Childrens Research Institute, Parkville, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia. Electronic address: - - 2014
Undescended testis (UDT) leads to infertility and malignancy resulting from aberrant germ cell (GC) development. Androgens are proposed to control early GC development during the transient postnatal surge of gonadotropins and androgen, known as mini-puberty. We aim to assess the effect of androgen receptor on perinatal GC development in mice. ...
Skuk Daniel - - 2014
The main challenge of cell transplantation as a treatment of myopathies is the large amount of tissue to treat. Intravascular delivery of cells may be an ideal route if proven to be effective and safe. Given the importance of nonhuman primates for preclinical research in transplantation, we tested the intra-arterial ...
Candeal Eduardo E University of - - 2014
Inorganic phosphate (Pi) transport in epithelia has both Na-dependent and Na-independent components, but so far only Na-dependent transporters have been characterized in detail and molecularly identified. Consequently, in this work we have initiated the characterization and analysis of intestinal Na-independent Pi transport using the in vitro model, Caco2BBE cells. Only ...
Jeong Su-Hyeon SH Department of Rehabilitation Medicine of Korean Medicine, Chungju Hospital of Korean Medicine, College of Korean Medicine, Semyung University, Jecheon 390‑711, Republic of - - 2014
Medicinal herbs used in traditional Oriental medicine, which have been in use clinically for thousands of years, are attractive sources of novel therapeutics or preventatives. Asiasari radix (A. radix) has been suggested for use in the treatment of dental diseases, including toothache and aphthous stomatitis. The aim of this study was ...
Gumucio Jonathan P JP University of - - 2014
Mechanical loading can increase tendon cross-sectional area (CSA), but the mechanisms by which this occurs are largely unknown. To gain a greater understanding of the cellular mechanisms of adult tendon growth in response to mechanical loading, we used a synergist ablation model whereby a tenectomy of the Achilles tendon was ...
Lee Won-Young WY Department of Food Bioscience, RIBH, College of Biomedical & Health Science, Konkuk University, Chung-ju 380-701, Republic of - - 2014
Spermatogenesis is initiated from spermatogonial stem cells (SSCs), which are derived from gonocytes. Although some rodent SSC markers have been investigated, other species- and developmental stage-specific markers of spermatogonia have not been identified. The objective of this study was to characterize the expression of undifferentiated embryonic cell transcription factor 1 ...
Abiuso Adriana María Belén AM A Abiuso, Lab of Molecular Endocrinology and Signal Transduction, IBYME-CONICET, Buenos Aires, - - 2014
The histamine H4 receptor (HRH4), discovered only thirteen years ago, is considered a promising drug target for allergy, inflammation, autoimmune disorders and cancer, as reflected by a steadily growing number of scientific publications and patent applications. Although there is evidence of its presence within the testis, neither its specific localization ...
Castello Pablo R PR Institute of Chemistry and Biological Physical Chemistry-Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina; Mechanical Engineering Department, University of Nevada Reno, Reno, - - 2014
This study presents experimental data for the effects of weak radio frequency (RF) magnetic fields on hydrogen peroxide (H2 O2 ) production and cellular growth rates of fibrosarcoma HT1080 cells in vitro. Cells were exposed either to 45 µT static magnetic fields (SMFs)-oriented vertical to the plane of growth or to ...
Skodje Anders A Faculty of Medicine and Dentistry, Department of Clinical Dentistry - Periodontics, University of Bergen, Bergen, - - 2014
Poly(L-lactide)-co-(epsilon-caprolactone) (poly(LLA-co-CL)) and poly(L-lactide)-co-(1,5-dioxepan-2-one) (poly(LLA-co-DXO)) are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 µg BMP-2 or without BMP-2 (control). ...
Schmidt A Mareike AM Institute of Molecular Systems Biology, ETH Zurich, CH-8093 Zurich, - - 2014
Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different ...
Wachter Britta B Institute of Neuroanatomy, University of Tübingen, Österbergstr. 3, 72074 Tübingen, - - 2014
Previously we have demonstrated that intraventricular injection of 6-hydroxydopamine (6-OHDA) results in increased proliferation and de-differentiation of rat cortical astrocytes into progenitor-like cells four days after lesion (Wachter et al., 2010). To find out if these cells express tyrosine hydroxylase (TH), the rate-limiting enzyme in the catecholamine synthesis pathway, we ...
Martín-Sabroso C C Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, - - 2014
To improve chemotherapy protocols of lymphoid malignancies, by using polymeric and lipid microparticles as controlled delivery systems of dexamethasone, part of all combined chemotherapy protocols for its strong-inducing effect on malignant lymphoblasts. Polymeric microparticles were prepared by the oil-in-water-emulsion cosolvent evaporation method, andlipid microparticles by spray drying. Their cytotoxic effects ...
Sonnaert M M Prometheus, Division of Skeletal Tissue Engineering, Katholieke Universiteit Leuven, Belgium; Department of Materials Engineering, Katholieke Universiteit Leuven, - - 2014
Perfusion bioreactor systems have shown to be a valuable tool for the in vitro development of three-dimensional (3D) cell-carrier constructs. Their use for cell expansion, however, has been much less explored. Since maintenance of the initial cell phenotype is essential in this process, it is imperative to obtain insight into ...
Meher Akshaya K AK Department of Surgery, University of Virginia, Charlottesville, - - 2014
Recent reports of rupture in patients with abdominal aortic aneurysm (AAA) receiving B-cell depletion therapy highlight the importance of understanding the role of B cells (B1 and B2 subsets) in the development of AAA. We hypothesized that B2 cells aggravate experimental aneurysm formation. The IHC staining revealed infiltration of B ...
Kim Su Jin SJ Department of Theriogenology and Biotechnology,College of Veterinary Medicine and the Research Institute for Veterinary Science,Seoul National University,Seoul 151-742,Republic of - - 2014
Summary Octamer-binding transcription factor 4 (Oct4) is a critical molecule for the self-renewal and pluripotency of embryonic stem cells. Recent reports have shown that Oct4 also controls cell-cycle progression and enhances the proliferation of various types of cells. As the high proliferation of donor fibroblasts is critical to the production ...
Ramuz Marc M Department of Bioelectronics, Ecole Nationale Supérieure des Mines CMP-EMSE, MOC, 880 avenue de Mimet, 13541, Gardanne, - - 2014
A planar, conducting-polymer-based transistor for combined optical and electronic monitoring of live cells provides a unique platform for monitoring the health of cells in vitro. Monitoring of MDCK-I epithelial cells over several days is shown, along with a demonstration of the device for toxicology studies, of use in future drug ...
Ishikawa Akira A Graduate School of Bioresources , MIE University , 1577 Kurima-Machiya-Cho, TSU, MIE 514-8507 , - - 2014
Temporal changes in the in situ germination flux of cysts and the abundance of vegetative cells of the toxic dinoflagellate Alexandrium catenella were investigated in Ago Bay, central Japan from July 2003 to December 2004. The in situ germination flux (cells m(-2) day(-1)) was measured using 'plankton emergence trap/chambers (PET ...
Okuno Tomofumi T Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan - - 2014
The aim of the present study was to clarify the mechanism underlying the inhibition of cell proliferation in human lung cancer A549 cells by selenium (Se) compounds. Methylseleninic acid (CH3SeO2H, abbreviated as MSA), a synthetic Se compound, is a direct precursor of active methylselenol (CH3SeH) and is considered to be ...
Lambricht Laure L Université catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, 1200 Brussels, - - 2014
The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs. Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties ...
Ekpenyong Christopher E CE 1 Department of Physiology, College of Health Sciences, University of Uyo , Uyo, Akwa Ibom State, Nigeria - - 2014
Abstract This study assessed the effects of lemongrass (Cymbopogon citratus) tea on hematologic indices in human volunteers. One hundred five subjects (55 men and 50 women), aged 18 to 35 years, were randomly assigned to groups set to orally receive infusion prepared from 2, 4, or 8 g of C. citratus ...
Martins Edivaldo - - 2014
BackgroundArticular cartilage, because of its avascular nature, has little capacity for spontaneous healing, and tissue engineering approaches, employing different biomaterials and cells, are under development. Among the investigated biomaterials are the chitosan-based hydrogels. Although thoroughly studied in other mammalian species, studies are scarce in equines. So, the aim of the ...
Wang Limin L Rice University, Department of Bioengineering , 6100 Main Street, MS-142 , Keck Hall, Room 116 , Houston, Texas, United States , 77005 , 713-348-3027 , 713-348-5877 ; - - 2014
In this work, we investigated a cytocompatible particulate leaching method for the fabrication of cell-laden macroporous hydrogels. We used dehydrated and uncrosslinked gelatin microspheres as leachable porogens to create macroporous oligo(poly(ethylene glycol) fumarate) hydrogels. Varying gelatin content and size resulted in a wide range of porosities and pore sizes, respectively. ...
Gencoglu Maria F - - 2014
Soft-templated mesoporous carbon is morphologically a non-nano type of carbon. It is a relatively newer variety biomaterial, which has already demonstrated its successful role in drug delivery applications. In order to investigate the toxicity and biocompatibility, we introduced three types of mesoporous carbons with varying synthesis conditions and pore textural ...
Hirata Azumi A Department of Medical and Life Science, Graduate School of Engineering, Osaka University, Suita , Osaka , Japan - - 2014
Abstract Using the proliferating cell nuclear antigen (PCNA) immunostaining, we previously identified, after pulp exposure, three zones of proliferating cells in the rat molar pulp. Zones I and II were in the crown near the pulp. Zone III was near the apex revealing a recruitment of mitotic cells at distance ...
Ishii Akihiro A Department of Oral and Maxillofacial Surgery, Tokyo Dental College , Chiba, - - 2014
We investigated the expression of p75(NGFR), a proliferative and basal cell marker, in the mouse buccal mucosa epithelium during wound healing in order to elucidate the role of epithelial stem cells. Epithelial defects were generated in the epithelium of the buccal mucosa of 6-week-old mice using CO2 laser irradiation. BrdU ...
Lv Peng P Cancer Institute and Hospital, Chinese Academy of Medical Sciences (CAMS), Beijing, People's Republic of - - 2014
Nasopharyngeal carcinoma (NPC), while uncommon worldwide, is a major health problem in China. Although local radiation and surgery provide good control of NPC, better treatments that permit reductions in radiation dosing are needed. Inhibition of protein phosphatase 2A (PP2A), a ubiquitous multifunctional enzyme with critical roles in cell cycle regulation ...
Marsilio S S Department of Pathology, University of Veterinary Medicine Hannover, Hannover, - - 2014
The distribution and numbers of CD3(+) T lymphocytes, immunoglobulin(+) plasma cells and calprotectin (L1)(+) macrophages was analyzed in full-thickness, formalin-fixed biopsy samples from the small intestine (duodenum, jejunum and ileum) and from the colon from nine cats with clinical signs of inflammatory bowel disease (IBD). All animals had lymphoplasmacytic enteritis ...
Tuo Wei-Wei WW Department of Biomedical Engineering, Ji Nan University, Guang Zhou, - - 2014
Numerous studies have suggested that transfusion of red blood cells (RBCs) stored over a long period of time may induce harmful effects due to storage-induced lesions. However, the underlying mechanisms responsible for this damage have not been identified. Furthermore, it is unclear why and how up to 30% of long-stored ...
Choong Pei Feng PF 1. MAKNA Cancer Research Institute, Kuala Lumpur, Malaysia; ; 2. Faculty of Medicine and Health Sciences, University Tunku Abdul Rahman (UTAR), Selangor, - - 2014
Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed ...
Hoentsch Maxi M Department of Cell Biology, University Medical Center Rostock, Rostock, - - 2014
Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up ...
Singh Himanshu H Department of Chemical, Tata Institute of Fundamental Research, Mumbai, - - 2014
Cellular metabolite analyses by 13C-NMR showed that C. reinhardtii cells assimilate acetate at a faster rate in heterotrophy than in mixotrophy. While heterotrophic cells produced bicarbonate and CO2aq, mixotrophy cells produced bicarbonate alone as predominant metabolite. Experiments with singly 13C-labelled acetate (13CH3-COOH or CH3-13COOH) supported that both the 13C nuclei ...
Intarapat Sittipon S 0000-0002-5134-1441 Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, - - 2014
Gonadal development of quail embryos was examined histologically using histological and histochemical methods. In the present study, quail embryos were studied at various stages of incubation period based on phases of gonadogenesis. Germ cell migration was observed on day 3-4 but gonadal differentiation and gonadal function were observed on day ...
Albani Clara María CM Laboratorio de Zoonosis Parasitarias, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, B7602AYL Mar del Plata, Argentina ; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, - - 2014
Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus ...
Chen Lijuan L Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, China; Department of Medicine, University of Cincinnati, Cincinnati, Ohio, United States of - - 2014
Stem cell therapy has a potential for regenerating damaged myocardium. However, a key obstacle to cell therapy's success is the loss of engrafted cells due to apoptosis or necrosis in the ischemic myocardium. While many strategies have been developed to improve engrafted cell survival, tools to evaluate cell efficacy within ...
Abbasi Naghmeh N Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran ; Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, - - 2014
Assessments of cell reactions such as motility, orientation and activation to the topography of the substratum will assist with the fabrication of a proper implantable scaffold for future tissue engineering applications.The current challenge is to analyze the orientation effect of elecrospun nanofibers of poly (ε-caprolactone) (PCL) on viability and proliferation ...
Tsai Ju-Ying - - 2013
Abstract Elevated cathepsin S (Cat S) level is correlated with higher migration ability in tumor cells. This study investigates the inhibitory effect of novel synthetic α-ketoamide compounds on cathepsin activity and cancer cell migration. The effect of several α-ketoamide compounds on the activity of recombinant cathepsins (Cat S, Cat L ...
Singh Sushant - - 2013
Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Fourier transform infrared spectroscopy revealed a spherical-shaped nanosphere in a size range of 812 ± 64 nm with ...
Gal A - - 2013
This report describes the microscopical and immunohistochemical characteristics of littoral cell angiosarcoma in a 12-year-old, neutered female, beagle dog. The dog succumbed to metastatic disease 3 months after diagnosis of a mid-splenic mass. The tumour was characterized by two histological patterns: anastomosing microvascular channels and microvascular papillary fronds. The neoplastic ...
Jewgenow K - - 2012
Teratospermia is a common phenomenon within felid species and has been attributed to reduction in genetic diversity. Testes from teratospermic domestic cats show enhanced spermatogenesis accompanied by remarkably reduced germ cell apoptosis. In the present study we investigated whether free-range teratospermic tom cats exhibit a similar testicular phenotype as proven ...
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