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Negro C L CL Laboratorio de Macrocrustáceos, Instituto Nacional de Limnología (CONICET-UNL), Ciudad Universitaria, Paraje El Pozo s/n, 3000 Santa Fe, Argentina; Escuela Superior de Sanidad, Facultad de Bioquímica y Ciencias Biológicas, UNL, Paraje El Pozo s/n, 3000 Santa Fe, Argentina. Electronic address: - - 2014
In this work, the effects of a pulse exposure of endosulfan on hepatopancreas, gills and ovary of the burrowing crab Zilchiopsis collastinensis were evaluated. The crabs were exposed to three sublethal concentrations in a pulse system with controlled dilutions. Water samples for pesticide concentrations measurements and crab tissue samples were ...
Jin Yong-Dong YD Department of Medical Oncology, Sichuan Cancer Hospital , Chengdu , China - - 2014
Abstract Context: Overexpression of SIRT1 is considered to enhance the resistance of HepG2 cells to irradiation. Shikonin, a naturally occurring naphthoquinone compound, displays anticancer effects and circumvents cancer drug resistance. Objectives: This study investigated the MDR reversal effect of shikonin induced by the overexpression of SIRT1. Materials and methods: The ...
Grant Daniel N DN School of Medicine, University of Missouri, Columbia, Missouri, - - 2014
Extremely low frequency electromagnetic fields (ELF-EMFs) can induce beneficial effects including enhanced protein synthesis and cell proliferation on healing bone and skin wounds. This study investigated the effects of ELF-EMFs on acellular tissue constructs with and without gold nanoparticles (AuNPs) to determine if cell proliferation could be increase and thus ...
Tallawi Marwa M cauerstrasse 6Erlangen, Germany , 91058 , +49(0)9131 85-20806 ; - - 2014
The present article investigates the use of a novel electrospun fibrous blend of poly(glycerol sebacate) (PGS) and poly(butylene succinate-co-butylene dilinoleate) (PBS-DLA) as a candidate for cardiac tissue engineering. Random electrospun fibers with various PGS/PBS-DLA compositions (70/30, 60/40, 50/50, and 0/100) were fabricated. In order to examine the suitability of these ...
Selokar N L NL Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, - - 2014
Cryopreservation of semen allows preservation of somatic cells, which can be used for the production of progeny through somatic cell nuclear transfer (SCNT). This approach could enable restoration of valuable high-genetic-merit progeny-tested bulls, which may be dead but the cryopreserved semen is available. We have successfully produced a live buffalo ...
Liang S S Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of - - 2014
After somatic cell nuclear transfer (SCNT), the epigenetic state of a differentiated donor cell nucleus must be reversed to the embryonic state. Incomplete epigenetic reprogramming and abnormal gene activation of the donor cell nuclei is thought to be the cause of low cloning efficiency. To improve cloning efficiency, we investigated ...
Sauvegarde C C Université Catholique de Louvain, Louvain-la-Neuve, - - 2014
Hox proteins are transcription factors known to be essential for embryo patterning. The detection of some Hox transcripts in oocytes and early embryos suggests that they could play a role before gastrulation. We previously demonstrated Hoxb9 expression in oocytes and from the zygote to the blastocyst stage in the mouse ...
Kang H Y HY College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South - - 2014
Steroidogenic factor 1 (SF-1) is essential for the development and function of steroidogenic tissues. Stable incorporation of SF-1 into embryonic stem cells has been reported to prime the cells for steroidogenesis. In this study, we obtained SF1 cDNA from mRNA of murine pituitary glands and constructed the SF1-expressing vector controlled ...
Alessio A A Department of Molecular Biology, FCEFQyN, National University of Río Cuarto, Río Cuarto, Córdoba, - - 2014
Transposon-mediated transgenesis is a well-established tool for genome manipulation in small animal models. However, translation of this active transgenesis method to the large animal setting requires further investigation. We have previously demonstrated that a helper-independent piggyBac (PB) transposon system can efficiently transpose transgenes into the bovine genome [Alessio et al. ...
Chanrot M M Department of Clinical Sciences, SLU, Uppsala, - - 2014
BoHV4 is a double-stranded DNA virus which has been associated to endometritis, metritis, and abortions in dairy cow. The objective of this study was to characterise its cytopathic effects on bovine endometrial epithelial cells (bEEC). Bovine uteri were collected from slaughter house and bEEC separated and cultivated as previously described ...
Teixeira Fábio G FG Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, - - 2014
It was recently shown that the conditioned media (CM) of Human Umbilical Cord Perivascular Cells (HUCPVCs), a mesenchymal progenitor population residing within the Wharton Jelly of the umbilical cord, was able to modulate in vitro the survival and viability of different neuronal and glial cells populations. In the present work, ...
Medina-Reyes Estefany I EI Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, CP 54059 Estado de México, - - 2014
Titanium dioxide nanoparticles (TiO2 NPs) studies have been performed using relatively high NPs concentration under acute exposure and limited studies have compared shape effects. We hypothesized that midterm exposure to low TiO2 NPs concentration in lung epithelial cells induces carcinogenic characteristics modulated partially by NPs shape. To test our hypothesis ...
Li Linxi L Center of Scientific Research, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, - - 2014
Diisononyl phthalate (DINP) is a synthetic material that has been widely used as a substitute for other plasticizers prohibited due to reproductive toxicity in consumer products. Some phthalates have been associated with testicular dysgenesis syndrome in male fetus when female pregnant dams were exposed to them. The present study investigated ...
Repin V S VS Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, - - 2014
We developed an original reproducible 3D-technology for preparation of single dormant microspheres consisting of 2000 somatic cells. The dynamics of microsphere assembly from mesenchymal and epithelial cells of retinal pigment epithelium was traced using time-lapse microscopy: formation of a loose aggregate over 24 h followed by its gradual consolidation and ...
Lungova Vlasta V Division of Otolaryngology Head and Neck Surgery, Department of Surgery, University of Wisconsin Madison, Madison, WI, 53705, - - 2014
Vocal fold epithelial cells are very difficult to study as the vocal fold epithelial cell lines do not exist and they cannot be removed from the healthy larynx without engendering a significant and unacceptable risk to vocal fold function. Here, we describe the procedure to create an engineered vocal fold ...
Xu Manlong M Optometry and Vision Science, University of Waterloo, Waterloo, ON, N2L 3G1, Canada. Electronic address: - - 2014
PrestoBlue is a new resazurin based reagent to assess cell viability and cytotoxicity. It is claimed to be a fast and highly sensitive assay. Here, we compared PrestoBlue, alamarBlue, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazonium bromide (MTT) in assessing cell viability of human corneal epithelial cells (HCEC), and investigated the effect of plate ...
Zhang Yu Y Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, - - 2014
Our previous studies have shown that podocyte number is significantly decreased in glomeruli of children with hepatitis B virus (HBV)-associated glomerulonephritis. In this study, we aimed to explore whether exogenous expression of HBx protein could directly inhibit podocyte proliferation in vitro, and to investigate its role in cell cycle regulation. ...
Kumari Archana A University of - - 2014
Taste sensation on the anterior tongue requires chorda tympani nerve function and connections with continuously renewing taste receptor cells. However, it is unclear which signaling pathways regulate the receptor cells to maintain chorda tympani sensation. Hedgehog (HH) signaling controls cell proliferation and differentiation in numerous tissues and is active in ...
Bellenberg Sören S Fakultät für Chemie, Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen Universitätsstr 5, 45141, Essen, Germany, - - 2014
In this study, the process of pyrite colonization and leaching by three iron-oxidizing Acidithiobacillus species was investigated by fluorescence microscopy, bacterial attachment, and leaching assays. Within the first 4-5 days, only the biofilm subpopulation was responsible for pyrite dissolution. Pyrite-grown cells, in contrast to iron-grown cells, were able to oxidize iron(II) ...
Gugerell Alfred A Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria. Electronic address: - - 2014
Generation of adipose tissue for burn patients that suffer from an irreversible loss of the hypodermis is still one of the most complex challenges in tissue engineering. Electrospun materials with their micro- and nanostructures are already well established for their use as extracellular matrix substitutes. Gelatin is widely used in ...
Sai Lin-Tao LT Department of Infectious Diseases, Qilu Hospital, Shandong University, Wenhua Xi Road 107, Ji'nan 250012, Shandong Province, - - 2014
Hepatitis B virus (HBV) infection is strictly species and tissue specific, therefore none of the cell models established previously can reproduce the natural infection process of HBV in vitro. The aim of this study was to establish a new cell line that is susceptible to HBV and can support the replication ...
Yun Jieun J 1 Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology , Ochang, Republic of - - 2014
Abstract This study was performed to elucidate the effect of a lipid-soluble ginseng extract (LSGE) on cancer invasion and metastasis. The LSGE, even at noncytotoxic concentrations, potently inhibited invasion and migration of B16F10 mouse melanoma cells in a dose-dependent manner. In the presence of 3 μg/mL of LSGE, the invasion ...
Rauš Balind Snežana S Institute for Biological Research Siniša Stanković, University of Belgrade, Belgrade, - - 2014
The aim of the present study was to determine does extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affect pituitary adrenocorticotroph (ACTH) cells in adult animals. We performed two series of experiments: (1) short-term exposure of 3-month-old rats to ELF-MF for 1 and 7 days, and (2) long-term ...
Zhang Kexiong K Institute of Aging Research, Hangzhou Normal University School of Medicine, No.1378, Wen-Yi West Road, Hangzhou, 311121, Zhejiang Province, - - 2014
Cellular senescence protects multicellular organisms from tissue overgrowth including cancer, and contributes to tissue ageing. With stable cell cycle arrests and particular molecular features, cellular senescence has been mostly studied in the adult tissues of animals including mammals. In the present study, we report a widespread of cellular senescence in ...
Sankar Rathinam K RK Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan Pune 411 008, Maharashtra, India. - - 2014
A thiol-selective 2-methyl-3-phenacrylate scaffold with spatiotemporal control over delivery of a cargo is reported. The half-lives of decomposition could be tuned from 30 min to 1 day and the scaffold's utility in thiol-inducible fluorophore release in cell-free as well as within cells is demonstrated.
Pulikkot S S Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, United Arab - - 2014
To generate various polycaprolactone (PCL) scaffolds and test their suitability for growth and differentiation of immortalized mouse gastric stem (mGS) cells. Non-porous, microporous and three-dimensional electrospun microfibrous PCL scaffolds were prepared and characterized for culture of mGS cells. First, growth of mGS cells was compared on these different scaffolds after ...
Bernardini André A University of - - 2014
Reactive oxygen species (ROS) mainly originating from NADPH oxidases have been shown to be involved in the carotid body (CB) oxygen sensing cascade. For measuring ROS kinetics type I cells of the mouse CB in an ex vivo preparation were transfected with the ROS sensor construct FRET-HSP33. After 2 days ...
Li Li L Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, P.R. - - 2015
Microglia and Müller cells are glial cells of the retina and constitute a functional link between neurons and vessels. The aim of the present study was to introduce a novel method of co-culture with Müller cells and microglia in rat retina. A camera was used to analyze all the cell ...
Li Ruili R Douglas Stephens Surgical Research laboratory, Murdoch Childrens Research Institute, Parkville, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Victoria 3052, Australia. Electronic address: - - 2014
Undescended testis (UDT) leads to infertility and malignancy resulting from aberrant germ cell (GC) development. Androgens are proposed to control early GC development during the transient postnatal surge of gonadotropins and androgen, known as mini-puberty. We aim to assess the effect of androgen receptor on perinatal GC development in mice. ...
Skuk Daniel - - 2014
The main challenge of cell transplantation as a treatment of myopathies is the large amount of tissue to treat. Intravascular delivery of cells may be an ideal route if proven to be effective and safe. Given the importance of nonhuman primates for preclinical research in transplantation, we tested the intra-arterial ...
Candeal Eduardo E University of - - 2014
Inorganic phosphate (Pi) transport in epithelia has both Na-dependent and Na-independent components, but so far only Na-dependent transporters have been characterized in detail and molecularly identified. Consequently, in this work we have initiated the characterization and analysis of intestinal Na-independent Pi transport using the in vitro model, Caco2BBE cells. Only ...
Jeong Su-Hyeon SH Department of Rehabilitation Medicine of Korean Medicine, Chungju Hospital of Korean Medicine, College of Korean Medicine, Semyung University, Jecheon 390‑711, Republic of - - 2014
Medicinal herbs used in traditional Oriental medicine, which have been in use clinically for thousands of years, are attractive sources of novel therapeutics or preventatives. Asiasari radix (A. radix) has been suggested for use in the treatment of dental diseases, including toothache and aphthous stomatitis. The aim of this study was ...
Gumucio Jonathan P JP Department of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan and Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, - - 2014
Mechanical loading can increase tendon cross-sectional area (CSA), but the mechanisms by which this occurs are largely unknown. To gain a greater understanding of the cellular mechanisms of adult tendon growth in response to mechanical loading, we used a synergist ablation model whereby a tenectomy of the Achilles tendon was ...
Lee Won-Young WY Department of Food Bioscience, RIBH, College of Biomedical & Health Science, Konkuk University, Chung-ju 380-701, Republic of - - 2014
Spermatogenesis is initiated from spermatogonial stem cells (SSCs), which are derived from gonocytes. Although some rodent SSC markers have been investigated, other species- and developmental stage-specific markers of spermatogonia have not been identified. The objective of this study was to characterize the expression of undifferentiated embryonic cell transcription factor 1 ...
Gasperin Bernardo G BG Laboratory of Animal Reproduction-ReproPEL, Department of Animal Pathology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; Department of Animal Science, McGill University, Sainte Anne de Bellevue, Quebec, - - 2014
Follicle development is coordinated by gonadotropins, steroids, and growth factors, which activate multiple signaling pathways. Phosphorylated-MAPK (pMAPK) level was indicated as an early marker of follicle dominance, whereas phosphorylated STAT3 (pSTAT3) was increased in granulosa cells of hypophysectomized rats. We hypothesized that MAPK3/1 and STAT3 pathways are regulated in granulosa ...
Abiuso Adriana María Belén AM A Abiuso, Lab of Molecular Endocrinology and Signal Transduction, IBYME-CONICET, Buenos Aires, - - 2014
The histamine H4 receptor (HRH4), discovered only thirteen years ago, is considered a promising drug target for allergy, inflammation, autoimmune disorders and cancer, as reflected by a steadily growing number of scientific publications and patent applications. Although there is evidence of its presence within the testis, neither its specific localization ...
Castello Pablo R PR Institute of Chemistry and Biological Physical Chemistry-Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina; Mechanical Engineering Department, University of Nevada Reno, Reno, - - 2014
This study presents experimental data for the effects of weak radio frequency (RF) magnetic fields on hydrogen peroxide (H2 O2 ) production and cellular growth rates of fibrosarcoma HT1080 cells in vitro. Cells were exposed either to 45 µT static magnetic fields (SMFs)-oriented vertical to the plane of growth or to ...
Skodje Anders A Faculty of Medicine and Dentistry, Department of Clinical Dentistry - Periodontics, University of Bergen, Bergen, - - 2014
Poly(L-lactide)-co-(epsilon-caprolactone) (poly(LLA-co-CL)) and poly(L-lactide)-co-(1,5-dioxepan-2-one) (poly(LLA-co-DXO)) are being considered candidate scaffolds for bone tissue engineering. We evaluated the bioactive potential of poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds loaded with low-dose bone morphogenetic protein-2 (BMP-2). Periodontal ligament (PDL) cells were cultured onto the various scaffolds loaded with 1 µg BMP-2 or without BMP-2 (control). ...
Schmidt A Mareike AM Institute of Molecular Systems Biology, ETH Zurich, CH-8093 Zurich, - - 2014
Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different ...
Wachter Britta B Institute of Neuroanatomy, University of Tübingen, Österbergstr. 3, 72074 Tübingen, - - 2014
Previously we have demonstrated that intraventricular injection of 6-hydroxydopamine (6-OHDA) results in increased proliferation and de-differentiation of rat cortical astrocytes into progenitor-like cells four days after lesion (Wachter et al., 2010). To find out if these cells express tyrosine hydroxylase (TH), the rate-limiting enzyme in the catecholamine synthesis pathway, we ...
Martín-Sabroso C C Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040, Madrid, - - 2014
To improve chemotherapy protocols of lymphoid malignancies, by using polymeric and lipid microparticles as controlled delivery systems of dexamethasone, part of all combined chemotherapy protocols for its strong-inducing effect on malignant lymphoblasts. Polymeric microparticles were prepared by the oil-in-water-emulsion cosolvent evaporation method, andlipid microparticles by spray drying. Their cytotoxic effects ...
Sonnaert M M Prometheus, Division of Skeletal Tissue Engineering, Katholieke Universiteit Leuven, Belgium; Department of Materials Engineering, Katholieke Universiteit Leuven, - - 2014
Perfusion bioreactor systems have shown to be a valuable tool for the in vitro development of three-dimensional (3D) cell-carrier constructs. Their use for cell expansion, however, has been much less explored. Since maintenance of the initial cell phenotype is essential in this process, it is imperative to obtain insight into ...
Meher Akshaya K AK Department of Surgery, University of Virginia, Charlottesville, - - 2014
Recent reports of rupture in patients with abdominal aortic aneurysm (AAA) receiving B-cell depletion therapy highlight the importance of understanding the role of B cells (B1 and B2 subsets) in the development of AAA. We hypothesized that B2 cells aggravate experimental aneurysm formation. The IHC staining revealed infiltration of B ...
Kim Su Jin SJ Department of Theriogenology and Biotechnology,College of Veterinary Medicine and the Research Institute for Veterinary Science,Seoul National University,Seoul 151-742,Republic of - - 2014
Summary Octamer-binding transcription factor 4 (Oct4) is a critical molecule for the self-renewal and pluripotency of embryonic stem cells. Recent reports have shown that Oct4 also controls cell-cycle progression and enhances the proliferation of various types of cells. As the high proliferation of donor fibroblasts is critical to the production ...
Ramuz Marc M Department of Bioelectronics, Ecole Nationale Supérieure des Mines CMP-EMSE, MOC, 880 avenue de Mimet, 13541, Gardanne, - - 2014
A planar, conducting-polymer-based transistor for combined optical and electronic monitoring of live cells provides a unique platform for monitoring the health of cells in vitro. Monitoring of MDCK-I epithelial cells over several days is shown, along with a demonstration of the device for toxicology studies, of use in future drug ...
Ishikawa Akira A Graduate School of Bioresources , MIE University , 1577 Kurima-Machiya-Cho, TSU, MIE 514-8507 , - - 2014
Temporal changes in the in situ germination flux of cysts and the abundance of vegetative cells of the toxic dinoflagellate Alexandrium catenella were investigated in Ago Bay, central Japan from July 2003 to December 2004. The in situ germination flux (cells m(-2) day(-1)) was measured using 'plankton emergence trap/chambers (PET ...
Okuno Tomofumi T Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan - - 2014
The aim of the present study was to clarify the mechanism underlying the inhibition of cell proliferation in human lung cancer A549 cells by selenium (Se) compounds. Methylseleninic acid (CH3SeO2H, abbreviated as MSA), a synthetic Se compound, is a direct precursor of active methylselenol (CH3SeH) and is considered to be ...
Lambricht Laure L Université catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, 1200 Brussels, - - 2014
The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs. Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties ...
Ekpenyong Christopher E CE 1 Department of Physiology, College of Health Sciences, University of Uyo , Uyo, Akwa Ibom State, Nigeria - - 2014
Abstract This study assessed the effects of lemongrass (Cymbopogon citratus) tea on hematologic indices in human volunteers. One hundred five subjects (55 men and 50 women), aged 18 to 35 years, were randomly assigned to groups set to orally receive infusion prepared from 2, 4, or 8 g of C. citratus ...
Martins Edivaldo - - 2014
BackgroundArticular cartilage, because of its avascular nature, has little capacity for spontaneous healing, and tissue engineering approaches, employing different biomaterials and cells, are under development. Among the investigated biomaterials are the chitosan-based hydrogels. Although thoroughly studied in other mammalian species, studies are scarce in equines. So, the aim of the ...
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