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Alme Charlotte B CB Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, 7491 Trondheim, - - 2014
The contribution of hippocampal circuits to high-capacity episodic memory is often attributed to the large number of orthogonal activity patterns that may be stored in these networks. Evidence for high-capacity storage in the hippocampus is missing, however. When animals are tested in pairs of environments, different combinations of place cells ...
Kammerer Axel A Department Biologie II, Ludwig-Maximilians-Universität München, Planegg, Germany; Graduate School for Systemic Neurosciences, Ludwig-Maximilians-Universität München, Planegg, - - 2014
Grid cells in the medial entorhinal cortex encode space with firing fields that are arranged on the nodes of spatial hexagonal lattices. Potential candidates to read out the space information of this grid code and to combine it with other sensory cues are hippocampal place cells. In this paper, we ...
El-Said Waleed Ahmed WA Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-ro, Mapo-Gu, Seoul 121-742, Republic of Korea; Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, - - 2014
Probing the local environment of target cells has been considered a challenging task due to the complexity of living cells. Here, we developed new single cell-based chip to investigate the intracellular and extracellular redox state of PC12 cells using spectroelectrochemical tool that combined surface-enhanced Raman scattering (SERS) and linear sweep ...
Vignardi Caroline P CP Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: - - 2014
Nanoparticles have physicochemical characteristics that make them useful in areas such as science, technology, medicine and in products of everyday use. Recently the manufacture and variety of these products has grown rapidly, raising concerns about their impact on human health and the environment. Adverse effects of exposure to nanoparticles have ...
Aranyosi Alexander J AJ BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA. - - 2014
After more than 50 years of debates, the role of spatial and temporal gradients during cell chemotaxis is still a contentious matter. One major challenge is that when cells move in response to a heterogeneous chemical environment they are exposed to both spatial and temporal concentration changes. Even in the ...
Bain Lauren E LE UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University, 911 Partners Way, Engineering Building 1, Raleigh, NC, 27603, - - 2014
Developing functional biomedical devices based on semiconductor materials requires an understanding of interactions taking place at the material-biosystem interface. Cell behavior is dependent on the local physicochemical environment. While standard routes of material preparation involve chemical functionalization of the active surface, this review emphasizes both biocompatibility of unmodified surfaces as ...
Heys James G JG Department of Neurobiology, Northwestern University, Evanston, IL 60208, - - 2014
Establishing how grid cells are anatomically arranged, on a microscopic scale, in relation to their firing patterns in the environment would facilitate a greater microcircuit-level understanding of the brain's representation of space. However, all previous grid cell recordings used electrode techniques that provide limited descriptions of fine-scale organization. We therefore developed ...
Charras Guillaume G 1] London Centre for Nanotechnology, 17-19 Gordon Street, London WC1H 0AH, UK. [2] Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, - - 2014
The way in which a cell migrates is influenced by the physical properties of its surroundings, in particular the properties of the extracellular matrix. How the physical aspects of the cell's environment affect cell migration poses a considerable challenge when trying to understand migration in complex tissue environments and hinders ...
Reece Sarah E SE Centre for Immunity, Infection & Evolution, Institutes of Evolution, Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FL, Scotland, UK. Electronic address: - - 2014
Life in seasonal environments often means facing extreme environmental fluctuations. Many multicellular organisms have evolved strategies to cope with this lifestyle. Single-celled malaria parasites are no different. An elegant experiment reveals that they respond to the availability of mosquitoes to make the most of seasonal transmission opportunities.
Kaneko Hiroki H Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, - - 2014
The position of the pelvic fins among teleost fishes has tended to shift rostrally during evolution. This positional shift seems to have led to the diversification of feeding behavior and allowed adaptation to new environments. To understand the developmental basis of this shift in pelvic fin position among teleosts, we ...
Dusny Christian C Department Solar Materials, Helmholtz Centre for Environmental Research GmbH - UFZ, Permoserstr. 15, Leipzig, DE 04318, Germany; Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, Emil-Figge-Str. 66, D-44227 Dortmund, - - 2014
Life is based on the cell as the elementary replicative and self-sustaining biological unit. Each single cell constitutes an independent and highly dynamic system with a remarkable individuality in a multitude of physiological traits and responses to environmental fluctuations. However, with traditional population-based cultivation set-ups, it is not possible to ...
Lelle Marco M Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, - - 2014
In this study we describe the synthesis of novel functional non-nucleoside adenylyl cyclase inhibitors, which can be easily modified with thiol containing biomolecules such as tumour targeting structures. The linkage between inhibitor and biomolecule contains cleavable bonds to enable efficient intracellular delivery in the reductive milieu of the cytosol as ...
Linder Stefan S Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Eppendorf, Martinistr. 52, 20246, Hamburg, Germany, - - 2014
Podosomes are adhesion and invasion structures that are particularly prominent in cells of the monocytic lineage such as macrophages, dendritic cells, and osteoclasts. They are multifunctional organelles that combine several key abilities required for cell migration and invasion. The podosome repertoire includes well-established functions such as cell-substrate adhesion, and extracellular ...
Nijenhuis Nadja N School of Materials, Faculty of Engineering and Physical Sciences, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom; Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, United - - 2014
Knowledge of how the elastic stiffness of a cell affects its communication with its environment is of fundamental importance for the understanding of tissue integrity in health and disease. For stiffness measurements, it has been customary to quote a single parameter quantity, e.g., Young's modulus, rather than the minimum of ...
Preston Marnie A MA Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, - - 2014
Myelin plays a critical role in proper neuronal function by providing trophic and metabolic support to axons and facilitating energy-efficient saltatory conduction. Myelination is influenced by numerous molecules including growth factors, hormones, transmembrane receptors and extracellular molecules, which activate signaling cascades that drive cellular maturation. Key signaling molecules and downstream ...
Thabet Sana S Université de Lyon, Université Lyon 1, CNRS-UCB-INSA, UMR 5240 Microbiologie, Adaptation et Pathogénie, Génétique Moléculaire des Levures, Domaine scientifique de la Doua, 10 rue Raphaël Dubois, bâtiment Lwoff, F-69626 Villeurbanne, France Université de Lyon, Université Lyon 1, CNRS, UMR 5256, IRCELYON, Institut de Recherches sur la Catalyse et l'Environnement de Lyon, 2 avenue Albert Einstein, F-69626 Villeurbanne, - - 2014
We have investigated the antimicrobial effects of photocatalysis on the yeast model Saccharomyces cerevisiae. To accurately study the antimicrobial mechanisms of the photocatalytic process, we focused our investigations on two questions: the entry of the nanoparticles in treated cells and the fate of the intracellular environment. Transmission electronic microscopy (TEM) ...
Gallo Vittorio V Center for Neuroscience Research, Children's National Medical Center, Washington, DC, 20010, USA - - 2014
What makes quiescent glial cells in the CNS convert into the most aggressive of all glial-derived tumors? Contrary to assumptions long past, CNS cells are exquisitely responsive to changes in their physiological surroundings during development and disease. Far from being a settled environment, the intricate cerebral landscape continuously bombards cells ...
Jiang Wenzhi - - 2014
BackgroundCell walls are essential for most bacteria, archaea, fungi, algae and land plants to provide shape, structural integrity and protection from numerous biotic and abiotic environmental factors. In the case of eukaryotic algae, relatively little is known of the composition, structure or mechanisms of assembly of cell walls in individual ...
Munari Marco M Department of Biology, University of Padua, Padua, - - 2014
The increasing use of nanotechnologies will lead to significant releases of engineered nanoparticles into the aquatic environment, where their impact is still poorly characterized. In the present paper, the genotoxic and cytotoxic properties of CdS quantum dots (QDs) and silver sulphide (Ag2S) coated with methyl polyethylene glycol (M-PEG) were investigated ...
Moeendarbary Emad - - 2014
Cells generate and sustain mechanical forces within their environment as part of their normal physiology. They are active materials that can detect mechanical stimulation by the activation of mechanosensitive signaling pathways, and respond to physical cues through cytoskeletal re-organization and force generation. Genetic mutations and pathogens that disrupt the cytoskeletal ...
Davidson Patricia M PM Weill Institute for Cell and Molecular Biology, Department of Biomedical Engineering Cornell University, Ithaca, NY 14853; - - 2014
Cell motility plays a critical role in many physiological and pathological settings, ranging from wound healing to cancer metastasis. While cell migration on 2-dimensional (2-D) substrates has been studied for decades, the physical challenges cells face when moving in 3-D environments are only now emerging. In particular, the cell nucleus, ...
Bell Eric L EL Massachusetts Institute of Technology, Department of Biology, Glenn Laboratory for the Science of Aging, Cambridge, MA 02139, - - 2014
Sirtuins are NAD(+)-dependent deacylases that regulate numerous biological processes in response to the environment. SirT1 is the mammalian ortholog of yeast Sir2, and is involved in many metabolic pathways in somatic tissues. Whole body deletion of SirT1 alters reproductive function in oocytes and the testes, in part caused by defects ...
Nagai Hiroki H International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka - - 2014
Legionella are gram-negative bacteria ubiquitously found in freshwater and soil environments. Once inhaled by humans, Legionella infection could result in a severe form of pneumonia known as Legionellosis. Legionella translocate ~300 effector proteins into host cells via the Dot/Icm type IV secretion system, which is central to Legionella pathogenesis. Here ...
Bisaria Anjali A Lewis-Sigler Institute for Integrative Genomics, Princeton University, 120 Carl Icahn Laboratory, Washington Road, Princeton, NJ, - - 2014
Microfluidic platforms are ideal for generating dynamic temporal and spatial perturbations in extracellular environments. Single cells and organisms can be trapped and maintained in microfluidic platforms for long periods of time while their responses to stimuli are measured using appropriate fluorescence reporters and time-lapse microscopy. Such platforms have been used ...
PerliƄska Agata A Centre of New Technologies, University of Warsaw , Banacha, Warsaw , - - 2014
Evolutionary advantages over cousin cells in bacterial pathogens may decide about the success of a specific cell in its environment. Bacteria use a plethora of methods to defend against other cells and many devices to attack their opponents when competing for resources. Bacteriocins are antibacterial proteins that are used to ...
Diepenbruck Carolin - - 2012
Selection of lead candidates in drug discovery is a complex and time-consuming process. Here, we describe an approach that allows prediction of the productivity and quality of recombinant proteins by stable producer cell clones with the help of transient transfection studies. This is exemplified for three distinct bispecific T cell ...
Codina Elisabet - - 2012
Pelvic fins in Ophidion rochei are reduced to four rod-like structures situated at the ventral jaws. While the fish is swimming, they make continuous sweeping movements on the bottom. This paper examines and describes the anatomy of the pelvic fins to determine the possible functions of these appendages in relation ...
Bauer Michael - - 2012
Cell-based microarrays are being increasingly used as a tool for combinatorial and high throughput screening of cellular microenvironments. Analysis of microarrays requires several steps, including microarray imaging, identification of cell spots, quality control, and data exploration. While high content image analysis, cell counting, and cell pattern recognition methods are established, ...
Welch G Rickey - - 2012
Recent investigations give reason to question anew the historical status of the 'cell theory' as the ultimate driving force in the development of our understanding of life's processes at the most fundamental level. A revisitation of critical research papers and commentaries from the 19th Century shows that the disregarded (and ...
Liang Dan - - 2012
BACKGROUND: β-Elemene, isolated from more than 50 Chinese herbs and plants, has shown promising anticancer effects against a broad spectrum of tumors, such as lung, breast, prostate, cervical, colon and ovarian carcinomas (Wang et al. in Cell Mol Life Sci 62:881-893, 2005; Li et al. in Cell Mol Life Sci ...
Xin-Zhao Wang Cynthia - - 2012
RPE cells are involved in the pathogenesis of many retinal diseases. Accurate analysis of RPE gene expression profiles in different scenarios will increase our understanding of disease mechanisms. Our objective in this study was to develop an improved method for the isolation of RPE cells, specifically for RNA analysis. Mouse ...
Carrara Paolo - - 2012
Current research on the origin of life typically focuses on the self-organisation of molecular components in individual cell-like compartments, thereby bringing about the emergence of self-sustaining minimal cells. This is justified by the fact that single cells are the minimal forms of life. No attempts have been made to investigate ...
Katoh Hiroto - - 2012
To maintain a functional and harmonious epithelial society, the number and quality of cells need to be tightly controlled. Two recent studies reveal a novel cellular process for epithelial homeostasis: crowding-mediated live cell extrusion.
Laudisi Federica - - 2012
Wireless local area networks are an increasing alternative to wired data networks in workplaces, homes, and public areas. Concerns about possible health effects of this type of signal, especially when exposure occurs early in life, have been raised. We examined the effects of prenatal (in utero) exposure to wireless fidelity ...
Mescola Andrea - - 2012
Single-cell force spectroscopy is an emerging technique in the field of biomedicine because it has proved to be a unique tool to obtain mechanical and functional information on living cells, with force resolution up to single molecular bonds. This technique was applied to the study of the cytoskeleton organisation of ...
Plášek Jaromír - - 2012
Fluorescence emission spectra of yeast cell suspensions stained with calcofluor have recently been identified as promising markers of variations in the quality of yeast cell wall. It is shown in this paper how the raw fluorescence spectra of calcofluor can be transformed to reliable spectral signatures of cell wall quality, ...
Raja Waseem Khan WK Tufts University Department of Biomedical Engineering 4 Colby Street Medford, Massachusetts - - 2012
Cancer cells create a unique microenvironment in vivo that enables migration to distant organs. To better understand the tumor micro-environment, special tools and devices are required to monitor the interactions between different cell types and the effects of particular chemical gradients. Our study presents the design and optimization of a ...
Bruno Robert D - - 2012
The capacity of any portion of the murine mammary gland to produce a complete functional mammary outgrowth upon transplantation to an epithelium-divested fat pad is unaffected by the age or reproductive history of the donor. Likewise, through serial transplantations, no loss of potency is detected when compared to similar transplantations ...
Bekei Beata - - 2012
Irrespective of how isotope-labeled proteins are delivered into mammalian cells, laboratory routines are needed to assess the quality of the resulting in-cell NMR samples. These include methods to evaluate overall cell viability, protein transduction efficiency, intracellular protein concentration, localization, and stability. In addition, quality control experiments to assess protein leakage ...
Savitsky Alexander P - - 2012
We report a new technique to detect enzyme activity inside cells. The method based on Fluorescence Lifetime Imaging (FLIM) technology allows one to follow sensor cleavage by proteolytic enzyme caspase-3. Specifically, we use the FLIM FRET of living cells via the confocal fluorescence microscopy. A specially designed lentivector pLVT with ...
Honnen Sebastian J - - 2012
The planar cell polarity (PCP) pathway is highly conserved from Drosophila to humans and a PCP-like pathway has recently been described in the nematode Caenorhabditis elegans. The developmental function of this pathway is to coordinate the orientation of cells or structures within the plane of an epithelium or to organize ...
Kato Gregory J - - 2012
Priapism is a familiar problem to hematologists, well known for its association with sickle-cell disease (SCD). It also occurs in a variety of other hematological illnesses, nearly all forms of congenital hemolytic anemia, including other hemoglobinopathies and red blood cell membranopathies and enzymopathies. Provide urologists with a comprehensive review of ...
Romero Diego D Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, - - 2011
Cells within Bacillus subtilis biofilms are held in place by an extracellular matrix that contains cell-anchored amyloid fibres, composed of the amyloidogenic protein TasA. As biofilms age they disassemble because the cells release the amyloid fibres. This release appears to be the consequence of incorporation of D-tyrosine, D-leucine, D-tryptophan and ...
Yuzbasheva Evgeniya Y - - 2011
The cell surface display of enzymes is of great interest because of its simplified purification stage and the possibility for recycling in industrial processes. In this study, we have focused on the cell wall immobilization of Yarrowia lipolytica Lip2 protein-an enzyme that has a wide technological application. By genome analysis ...
Zuckerman Warren A - - 2011
Pulmonary hypertension and cor pulmonale have long been known to be complications of sickle cell disease, thought mostly to affect the adult population. Recently, pediatric studies in sickle cell patients have uncovered a similar prevalence of elevated tricuspid regurgitation jet velocities, a finding consistent with increased right-sided pressures, when compared ...
Hooper Nigel M - - 2011
J. Neurochem. (2011) 116, 721-725. ABSTRACT: The conformational conversion of the cellular prion protein (PrP(C) ) to the infectious form (PrP(Sc) ) is the critical step in the pathogenesis of prion diseases such as Creutzfeldt-Jakob disease in humans and scrapie in sheep. Cholesterol-rich lipid rafts play a key role in ...
Dyson Simon M - - 2011
The experiences of young people living with a sickle cell disorder in schools in England are reported through a thematic analysis of forty interviews, using Bourdieu's notions of field, capital and habitus. Young people with sickle cell are found to be habitually dys-positioned between the demands of the clinic for ...
Bhatnagar Pallav - - 2011
Fetal hemoglobin (HbF) level has emerged as an important prognostic factor in sickle-cell disease (SCD) and can be measured by the proportion of HbF-containing erythrocytes (F-cells). Recently, BCL11A (zinc-finger protein) was identified as a regulator of HbF, and the strongest association signals were observed either directly for rs766432 or for ...
Sipling Tanya - - 2011
There are six essential genes in the S. cerevisae genome which encode proteins bearing the tetratricopeptide repeat (TPR) domain that mediates protein-protein interaction. Thus far, the function of one of them, YNL313c, remains unknown. Our conditional mutants of YNL313c display osmoremedial temperature sensitivity, hypersensitivity to both Calcofluor White and low ...
Lu Jingxiong - - 2011
In the present study, we fabricated magnesium doped apatite cement (md-AC) with rapid self-setting characteristic by adding the mixed powders of magnesium oxide and calcium dihydrogen phosphate (MO-CDP) into hydroxyapatite cement (HAC). The results revealed that the md-AC with 50 wt% MO-CDP could set within 6 min and the compression strength could ...
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