Search Results
Results 201 - 250 of 1837
< 1 2 3 4 5 6 7 8 9 10 >
Young Christian D CD Department of Pathology, Mailstop 8104, University of Colorado Denver, University of Colorado School of Medicine, P,O, Box 6511, Anshutz Medical Campus, Aurora, CO 80045, USA. - - 2009
The high rate of glucose utilization by cancer cells has been well characterized. Recent data suggest that when normal mammary epithelial cells are cultured under nonadherent conditions, glucose consumption decreases, ATP levels fall, and concentrations of reactive oxygen species rise. The rise in reactive oxygen species causes death of nonadherent ...
Shelton Melissa D - - 2009
Protein S-glutathionylation is a reversible redox-dependent post-translational modification. Many cellular functions and signal transduction pathways involve proteins whose cysteine-dependent activities are modulated by glutathionylation. Glutaredoxin (Grx1) plays a key role in such regulation because it is a specific and efficient catalyst of deglutathionylation. We recently reported an increase in Grx1 ...
Pyaskovskaya O N - - 2008
AIM: To analyze the growth kinetics and proliferative heterogeneity of Lewis lung carcinoma (LLC) cells during their growth in monolayer for 5 days without replacement of culture medium (unfed culture). METHODS: Cell biology methods, sandwich enzyme-linked immunosorbent assay for vascular endothelial growth factor (VEGF) detection (ELISA), enzymatic glucose-oxidase method for ...
Reimann Frank - - 2008
Glucagon-like peptide-1 (GLP-1) is an enteric hormone that stimulates insulin secretion and improves glycaemia in type 2 diabetes. Although GLP-1-based treatments are clinically available, alternative strategies to increase endogenous GLP-1 release from L cells are hampered by our limited physiological understanding of this cell type. By generating transgenic mice with ...
Cinar Kenan - - 2009
Branchial epithelium of Pseudophoxinus antalyae was lined by both a thick stratified epithelium lining gill arches, gill rakers and primary filaments and a thin epithelium lining the lamellae. Mucous, chloride and rodlet cells, interspersed between pavement cells, were present in the branchial epithelium. With histochemical procedures for the characterization of ...
Martins, Margarida Rangel
The success of Candida species as pathogens is attributed to survival strategies such as the ability to form biofilms. In fact, Candida albicans and Candida parapsilosis are frequently isolated solely or within mixed cultures from implanted devices and natural environments. Nevertheless, interactions between organisms, namely through signalling molecules, are not ...
Rollet Cécile - - 2009
Pseudomonas aeruginosa is a pathogenic bacterium widely investigated for its high incidence in clinical environments and its ability to form strong biofilms. During biofilm development, sessile cells acquire physiological characteristics differentiating them from planktonic cells. But after treatment with disinfectants, or to ensure survival of the species in hostile environments, ...
Mokhbi Soukane D - - 2009
As the disc is the largest avascular structure in the body, disc cells depend for their normal function on an adequate supply of nutrients (oxygen and glucose) and the removal of metabolic by-products (lactic acid) via blood vessels at the cartilaginous endplates and annulus periphery. Concentration gradients develop depending on ...
Kim Jaeeun - - 2009
OBJECTIVES: The aim of the study was to determine the susceptibility of active and dormant cell populations from Pseudomonas aeruginosa biofilms to non-antibiotic antimicrobial agents such as chlorine, hydrogen peroxide and silver ions in comparison with antibiotics. METHODS: Active cells in colony biofilm were differentially labelled by induction of a ...
Matsui Ken - - 2009
The cell wall of Saccharomyces cerevisiae plays an essential role in the biophysical characteristics of the cell surface. The modification of the cell wall property is an important factor for cellular adaptation to a stressful environment. In this study, we randomly modified the cell wall by displaying combinatorial random peptides ...
Vidro Eileen K - - 2008
PURPOSE: Determine the effects of glucose and exogenous TGFbeta2 on viability and VEGF release by human retinal pericytes (HRP). METHODS: Human retinal pericytes (HRP) were cultured in 5 mM (physiologic) or high (18 mM) glucose with or without added TGFbeta2. Viable cells were counted; TGFbeta2 and VEGF in the conditioned ...
Wang Niansong - - 2008
BACKGROUND: CD146 is a cell adhesion molecule belonging to the immunoglobulin superfamily. It plays a critical role in cell proliferation and activation, and contributes to inflammatory reactions. We studied the association between CD146 and renal tubular epithelial cells (RTECs) under normal (NG) and high glucose (HG) to understand its role ...
Hayata Kenji - - 2008
To obtain compounds that promote glucose uptake in muscle cells, the novel cell lines A31-IS derived from Balb/c 3T3 A31 and C2C12-IS from mouse myoblast C2C12 were established. In both cell lines, glucose consumption was induced by insulin and suppressed by the addition of Akt-activating kinase inhibitor. The A31-IS cells ...
Peeters E - - 2008
In the present study we evaluated the efficacy of various procedures recommended for the disinfection of respiratory equipment and other materials in cystic fibrosis, using both planktonic and sessile Burkholderia cenocepacia cells. A modified European Suspension Test was performed to determine the effects of the disinfection procedures on planktonic cells. ...
Seidler Karin - - 2009
Rapid advancements in biotechnology depend on the development of reliable sensing sytems adaptable to a variety of analytes. Molecularly imprinted polymers combined with mass-sensitive devices (10 MHz QCMs) provide a reliable and robust method to observe cell growth and allow cell specifications in real-time. Such biosensors selectively detect fermenting yeast ...
Ingham Colin J - - 2008
Within an isogenic microbial population in a homogenous environment, individual bacteria can still exhibit differences in phenotype. Phenotypic heterogeneity can facilitate the survival of subpopulations under stress. As the gram-positive bacterium Lactobacillus plantarum grows, it acidifies the growth medium to a low pH. We have examined the growth of L. ...
Eisenhut Marion - - 2008
The amount of inorganic carbon represents one of the main environmental factors determining productivity of photoautotrophic organisms. Using the model cyanobacterium Synechocystis sp. PCC 6803, we performed a first metabolome study with cyanobacterial cells shifted from high CO(2) (5% in air) into conditions of low CO(2) (LC; ambient air with ...
Gunawardena Anuradh - - 2008
Saccharomyces cerevisiae present in common Baker's yeast was used in a microbial fuel cell in which glucose was the carbon source. Methylene blue was used as the electronophore in the anode compartment, while potassium ferricyanide and methylene blue were tested as electron acceptors in the cathode compartment. Microbes in a ...
Yuan Zhilan - - 2009
Increased nitric oxide (NO) has been correlated with diabetic retinopathy. In this study we investigated the cell injury, production of NO in retinal pigment epithelial (RPE) cells exposed to increased glucose concentration, and its molecular mechanism involved. Cultured human RPE cells (ARPE-19) were exposed for 4 days with normal blood ...
Matoba Shogo - - 2008
In mouse sex differentiation, SRY promotes Sertoli cell differentiation via SOX9 action, resulting in testis formation. SRY/SOX9 also initiates various testis-specific morphogenic events including glycogenesis in pre-Sertoli cells, suggesting the importance of glucose storage for certain SRY/SOX9-downstream events in gonadal sex determination. However, it remains unclear which cell types and ...
Rorsman Patrik - - 2008
Glucagon, secreted by the alpha-cells of the pancreatic islets, is the most important glucose-increasing hormone of the body. The precise regulation of glucagon release remains incompletely defined but has been proposed to involve release of inhibitory factors from neighbouring beta-cells (paracrine control). However, the observation that glucose can regulate glucagon ...
Holian John - - 2008
Krüppel-like factor 6 (KLF6) is a DNA-binding protein containing a triple zinc-fingered motif and plays a key role in the regulation of cell proliferation, differentiation, and development. More recently it has been implicated in hepatic fibrosis via its binding to the transforming growth factor (TGF)-beta control element. In the kidney, ...
Sánchez Norma Silvia - - 2008
Debaryomyces hansenii was grown in YPD medium without or with 1.0 M NaCl or KCl. Respiration was higher with salt, but decreased if it was present during incubation. However, carbonylcyanide-3-chlorophenylhydrazone (CCCP) markedly increased respiration when salt was present during incubation. Salt also stimulated proton pumping that was partially inhibited by ...
Qu JunWei - - 2009
OBJECTIVE: To investigate whether insulin resistance (IR) within theca cells may directly contribute to their hyperandrogenism, a heritable trait of polycystic ovary syndrome (PCOS). DESIGN: In vitro cell model. SETTING: University-affiliated laboratory. ANIMAL(S): Porcine ovaries. INTERVENTION(S): Ovarian theca cells from porcine follicles were isolated and cultured. Insulin resistance was induced ...
Azzu Vian V MRC Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Cambridge, UK. - - 2008
Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and ...
Font de Mora, Jaime
Final full-text version of the paper available at: http://www.nature.com/emboj/journal/v22/n12/index.html
Gadau S - - 2008
The microtubular network of neurons is involved in several functions such as formation and tropism of cellular processes, cell division and intracellular transport. A lot of evidences testify that the microtubular network of neurons can be impaired by oxidative stress. A condition of oxidative stress is often possible when D-glucose ...
Gaisano Herbert Y - - 2008
Glucagon release by pancreatic alpha cells is stimulated by low glucose through unclear mechanisms. In this issue of Cell Metabolism, Cabrera et al. (2008) show that glutamate released from alpha cells acts on glutamate receptors in a positive autocrine fashion that drives glucagon secretion during small physiological fluctuations of blood ...
Fang Bo-yan - - 2008
BACKGROUND: Two novel presenilin 1 (PS1) mutations, V97L and A136G, were recently found to be involved in the early-onset of Alzheimer's disease in two Chinese families. This research aimed to verify their pathological effects. METHODS: The human neuroblastoma SH-SY5Y cells stably transfected with these two Chinese presenilin 1 mutations were ...
Malki Moustafa - - 2008
Several anaerobic metal-reducing bacteria have been shown to be able to donate electrons directly to an electrode. This property is of great interest for microbial fuel cell development. To date, microbial fuel cell design requires avoiding O(2) diffusion from the cathodic compartment to the sensitive anodic compartment. Here, we show ...
Yuneva Mariia - - 2008
Multiple lines of evidence indicate that the process of tumorigenesis is often associated with altered metabolism of two major nutrients, glucose and glutamine. These two nutrients are engaged in multiple metabolic pathways that can be required for cell viability. The roles of glucose and glutamine in the survival of transformed ...
Rusch Antje - - 2008
The microbial population of geothermally heated sediments in a shallow bay of Vulcano Island (Italy) was characterized with respect to metabolic activities and the putatively catalyzing hyperthermophiles. Site-specific anoxic culturing media, most of which were amended with combinations of electron donors (glucose or carboxylic acids) and acceptors (sulfate), were used ...
Jacobs Sarah R SR Department of Pharmacology and Cancer Biology, Sarah W Stedman Center for Nutrition and Metabolism, Duke University Medical Center, Durham, NC 27710, - - 2008
T cell activation potently stimulates cellular metabolism to support the elevated energetic and biosynthetic demands of growth, proliferation, and effector function. We show that glucose uptake is limiting in T cell activation and that CD28 costimulation is required to allow maximal glucose uptake following TCR stimulation by up-regulating expression and ...
Sousa, Cláudia
The aim of this work was to compare the glucose uptake of biofilms formed by four different Staphylococcus epidermidis strains as well as to compare between sessile and planktonic cells of the same strain. Biofilm cells showed a lower level of glucose uptake compared to planktonic cells. Moreover, glucose uptake ...
Bürgers Heinrich F HF Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany. - - 2008
Hypoxic-ischemic damage is a major challenge for neuronal tissue. In the present study, we investigated the effects of anoxia and glucose deprivation on adult neural stem cells (NSCs) in vitro. We assessed glucose deprivation, anoxia and the combination of the latter separately. After 24 h of anoxia, cell numbers increased ...
Oya-Ito Tomoko - - 2008
Diabetic patients are prone to severe bacterial infections. The functional alterations of neutrophils by hyperglycemia are thought to be partially responsible for such infections. In this study, we investigated the functional changes of neutrophil-like differentiated cell lines (dHL-60, dTHP-1, and dNB-4) by treatment with 5.5 mM, 11 mM, or 35 ...
Boutinaud M - - 2008
Feed restriction and once-daily milking (ODM) reduce milk yield in dairy cows and the amount of glucose taken up by the mammary gland. The modulation of mammary glucose uptake may be the consequence of modifications to glucose transport, capacity for lactose synthesis, and cell death in mammary epithelial cells (MEC). ...
Schild Lorenz - - 2008
A solid tumour undergoes ischemia/reperfusion due to deficient vascularization and subsequent formation of new blood vessels. This study investigated the effect of transient oxygen and glucose deprivation (OGD) on proliferation of C6 glioma cells. The cells were subjected to 18 h of OGD followed by reoxygenation in the presence of ...
Shi Zhiqi - - 2008
The effects of osthol, a plant coumarin, on morphology, sugar uptake and cell wall components of Fusarium graminearum were examined in vitro by electron microscopy,(14)C-labelling and enzyme activity detection. The results revealed that osthol could inhibit the hypha growth of F. graminearum by decreasing hyphal absorption to reducing sugar. After ...
Meadows Adam L - - 2008
The metabolic and morphological characteristics of two human epithelial breast cell populations--MCF7 cells, a cancerous cell line, and 48R human mammary epithelial cells (48R HMECs), a noncancerous, finite lifespan cell strain--were compared at identical growth rates. Both cell types were induced to grow rapidly in nutrient-rich media containing 13C-labeled glucose, ...
Kato Aki - - 2008
Mannose is an unusable carbon source for many plants. In our study we compared the effects of mannose and sucrose on growth and sucrose levels in azuki bean (Vigna angularis) cells grown in liquid media and in solid media. The suspension cells grew actively in a liquid medium containing 90 ...
Al-Dhaheri Rawya S - - 2008
Biofilms and planktonic cells of five Candida species were surveyed for the presence of persister (drug-tolerant) cell populations after exposure to amphotericin B. None of the planktonic cultures (exponential or stationary phase) contained persister cells. However, persisters were found in biofilms of one of two strains of Candida albicans tested ...
Zheng J-M - - 2008
Rhein, an anthraquinone compound isolated from rhubarb, has been proved effective in treatment of experimental diabetic nephropathy (DN). To explore the mechanism of its therapeutic effect on DN, rhein was tested for its effect on the hexosamine pathway. The influence of rhein on cellular hypertrophy, fibronectin synthesis, glucose uptake, glutamine: ...
PARK, Min Kyu
It has been proposed that reactive oxygen species (ROS), mainly superoxide anion (<TEX>$O_2^-$</TEX>) and hydrogen peroxide (<TEX>$H_2O_2$</TEX>), may mediate oxidative stress. Production of <TEX>$H_2O_2$</TEX> during oxidative phosphorylation, inflammation, and ischemia can cause oxidative stress leading to cell death. Although glucose oxidase (GOX) in the presence of glucose continuously generates <TEX>$H_2O_2$</TEX>, ...
Gopinath P - - 2008
The impact of manufactured nanomaterials on human health and the environment is a major concern for commercial use of nanotechnology based products. A judicious choice of selective usage, lower nanomaterial concentration and use in combination with conventional therapeutic materials may provide the best solution. For example, silver nanoparticles (Ag NPs) ...
Jaques Fabienne - - 2008
Cell-to-cell interactions play an important role in insulin secretion. Compared with intact islets, dispersed pancreatic beta-cells show increased basal and decreased glucose-stimulated insulin secretion. In this study, we used mouse MIN6B1 cells to investigate the mechanisms that control insulin secretion when cells are in contact with each other or not. ...
Sidorenko Yury - - 2008
In mammalian cell cultures, ammonia that is released into the medium as a result of glutamine metabolism and lactate that is excreted due to incomplete glucose oxidation are both known to essentially inhibit the growth of cells. For some cell lines, for example, hybridoma cells, excreted ammonia also has an ...
Wei Chun - - 2008
High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g l(-1), short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g l(-1). ...
Ispolnov K - - 2008
AIM: The purpose of this study was to investigate the behaviour of Saccharomyces cerevisiae in response to extracellular methylglyoxal. METHODS AND RESULTS: Cell survival to methylglyoxal and the importance of phosphates was investigated. The role of methylglyoxal detoxification systems and methylglyoxal-derived protein glycation were studied and the relation to cell ...
Clark, Melinda Erin
Desulfovibrio vulgaris Hildenborough is a member of the sulfate-reducing bacteria (SRB) and is considered a model microorganism. SRB are beneficial because of their potential for bioremediation applications and detrimental due to their ability to corrode metals. Therefore, not only is it important to understand the metabolic capacity of SRB but ...
< 1 2 3 4 5 6 7 8 9 10 >