Search Results
Results 201 - 250 of 1934
< 1 2 3 4 5 6 7 8 9 10 >
aalster and tzohary
Aulacoseira (formerly Melosira) granulata is one of the most common diatoms of Lake Kinneret. It is the major phytoplankton biomass contributor in winter (Dec -Feb), when the lake is homothermal and it occupies the entire 43 m water column. At the end of the winter bloom in early March the ...
Chen Hsiu-Hung - - 2007
An understanding of cell osmotic behavior and membrane transport properties is indispensable for cryobiology research and development of cell-type-specific, optimal cryopreservation conditions. A microfluidic perfusion system is developed here to measure the kinetic changes of cell volume under various extracellular conditions, in order to determine cell osmotic behavior and membrane ...
Robert Claude - - 2007
A new type of multiple-reflection optical cell is presented. One of the main advantages of this type of cell is that it can be made of standard mirrors without particular tolerance while allowing a great number of reflections and thus a large optical path, only limited by the reflection coefficient ...
Eng Ben B Department of Pharmacology, New York Medical College, Valhalla, NY 10595, - - 2007
A medullary thick ascending limb (mTAL) cell line, termed raTAL, has been established from freshly isolated rat mTAL tubules and cultured continuously for up to 75 passages; it retains characteristics of mTAL cells even after retrieval from storage in liquid nitrogen for several months. The cells express Tamm-Horsfall glycoprotein (THP), ...
McCoy Eric E Department of Neurobiology, Center for Glial Biology in Medicine, University of Alabama at Birmingham, Birmingham, Alabama, - - 2007
Aquaporins (AQP) constitute the principal pathway for water movement across biological membranes. Consequently, their expression and function is important for cell volume regulation. Glioma cells quickly adjust their cell volume in response to osmotic challenges or spontaneously as they invade into the narrow and tortuous extracellular spaces of the brain. ...
Maki Masahiro - - 2007
In the present study, we investigated if Cl(-) regulates the proliferation of the MC3T3-E1 osteoblastic cells. The proliferation of MC3T3-E1 osteoblastic cells was diminished by lowering the extracellular Cl(-) concentration ([Cl(-)](o)) in the culture medium. The lowered in [Cl(-)](o) increased the periods of the G(0)/G(1) and the G(2)/M phases in ...
Huang Chiun-Chien - - 2007
Cell volume decrease is a key step during differentiation of erythroid cells. This could arise from membrane transporter activation leading to a loss of cell osmolytes; however, the pathways involved are poorly understood. We have characterised Cl(-)-independent K(+) and (3)H-taurine efflux from the erythroleukemia cell line, K562. K(+) efflux (measured ...
Akimova Olga A - - 2008
The cytotoxic effect of long-term exposure of renal epithelial cells to ouabain and other cardiotonic steroids (CTS) is mediated by the interaction of these compounds with Na(+),K(+)-ATPase but is independent of the inhibition of Na(+),K(+)-ATPase-mediated ion fluxes. Sustained application of CTS also leads to Na(+),K(+)-ATPase endocytosis and its translocation into ...
Ross Sandra B - - 2007
Despite intensive research, brain tumors remain among the most difficult type of malignancies to treat, due largely to their diffusely invasive nature and the associated difficulty of adequate surgical resection. To migrate through the brain parenchyma and to proliferate, glioma cells must be capable of significant changes in shape and ...
Cooper Trevor G - - 2007
The osmotic challenges facing maturing spermatozoa and their responses to them are discussed in relation to the concept of sperm maturation, defined as the increased ability of more distally recovered epididymal spermatozoa to fertilize eggs when inseminated into the female tract. One explanation could be that the more distal cells ...
vanTol Brenna L - - 2007
Using the human mammary epithelial cell line MCF-7, we have investigated volume-activated changes in response to hyposmotic stress. Switching MCF-7 cells from an isosmotic to a hyposmotic solution resulted in an initial cell swelling response, followed by a regulatory volume decrease (RVD). This RVD response was inhibited by the nonselective ...
Jorgensen Paul - - 2007
It is not known how the volume of the cell nucleus is set, nor how the ratio of nuclear volume to cell volume (N/C) is determined. Here, we have measured the size of the nucleus in growing cells of the budding yeast Saccharomyces cerevisiae. Analysis of mutant yeast strains spanning ...
Obata Toshihiro - - 2007
We screened a rice (Oryza sativa L. 'Nipponbare') full-length cDNA expression library through functional complementation in yeast (Saccharomyces cerevisiae) to find novel cation transporters involved in salt tolerance. We found that expression of a cDNA clone, encoding the rice homolog of Shaker family K(+) channel KAT1 (OsKAT1), suppressed the salt-sensitive ...
Kim Jong-yoon - - 2007
The leaf-cutter bee, Megachile tsurugensis, builds a brood cell in a preexisting tunnel with leaf discs that she cuts in decreasing sizes and assembles them like a Russian matryoshka doll. By experimentally manipulating the brood cell, it was investigated how she regulates the size of leaf discs that fit in ...
Belsey Mark J - - 2007
Cell swelling is associated with the activation of an increase in the osmosensitive taurine release (OTR) rate, which serves to decrease cell volume as part of a process known as regulatory volume decrease. OTR, which is sensitive to many pharmacological agents including anion channel blockers and signalling pathway modulators, has ...
Yin W - - 2007
Human T-cell leukemia is a malignant disease that needs various regimens of cytotoxic chemotherapy to overcome drug resistance. Recently, Na(+),K(+)-ATPase has emerged as a potential target for cancer therapy. However, its exact signaling pathway in human T-cell leukemia cell death has not been well defined. In the current study, we ...
Nielsen Doris K - - 2007
The labelling pattern of cellular phosphoinositides (PtdInsP(n)) was studied in Ehrlich ascites cells labelled in vivo for 24 h with myo-[2-(3)H]- or l-myo-[1-(3)H]inositol and exposed to anisotonic or isosmotic volume perturbations. In parallel experiments the cell volume ([(14)C]3-OMG space) was monitored. In hypotonic media the cells initially swelled osmotically and ...
Günther Theodor - - 2007
Experiments, results and conclusions on Na+/Mg2, antiport in lymphocytes, HL60 cells, Ehrlich ascites tumor cells, platelets, pancreatic acinar cells, sublingual acini, hepatocytes, ruminal epithelial cells, kidney cells, smooth muscle cells, heart muscle cells and skeletal muscle cells were reviewed. Only in a few experiments was the Mg2+ efflux via the ...
Shen Chengcheng - - 2007
We describe a new assay to determine the fraction of cardiac Na(+)-Ca(2+) exchangers (NCX1) in the surface membrane of cells (F(surf)). An extracellular NCX1 disulphide bond is rapidly reduced by tris(2-carboxyethyl)phosphine hydrochloride (TCEP), cysteines are 'PEGylated' by alkylation with an impermeable conjugate of maleimide and a 5000 MW polyethylene glycol ...
Lee Robert J - - 2007
Airway submucosal glands are sites of high expression of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel and contribute to fluid homeostasis in the lung. However, the molecular mechanisms of gland ion and fluid transport are poorly defined. Here, submucosal gland serous acinar cells were isolated from murine airway, ...
Burt Sara A - - 2007
Carvacrol is known to inhibit a number of food borne pathogens. The activity of carvacrol vapour was evaluated against S. enterica serotype Enteritidis on tryptone soya agar and on pieces of raw chicken. On agar, the size of the inhibition zone increased with decreasing volume of agar, increasing vapour exposure ...
Chvátal Alexandr - - 2007
Pathological states in the central nervous system lead to dramatic changes in the activity of neuroactive substances in the extracellular space, to changes in ionic homeostasis and often to cell swelling. To quantify changes in cell morphology over a certain period of time, we employed a new technique, three-dimensional confocal ...
Koizumi Keiichi - - 2007
BACKGROUND: The aim of the present study was to examine whether stanniocalcin 1 (STC1) affects cardiomyocytes under physiological or pathophysiological conditions. METHODS AND RESULTS: Using fresh isolated rat cardiomyocytes, the effects of STC1 on cell hypercontracture, cell shortening and Ca(2+) transients were measured after exposing the cells to ouabain. STC1 ...
Habela Christa W CW Department of Neurobiology, Center for Glial Biology in Medicine, University of Alabama at Birmingham, Birmingham, Alabama, - - 2007
Cell growth and osmotic volume regulation are undoubtedly linked to the progression of the cell cycle as with each division, a newly generated cell must compensate for loss of half of its volume to its sister cell. The extent to which size influences cell cycle decisions, however, is controversial in ...
Chen L X - - 2007
OBJECTIVES: Previously it has been shown, that the volume-activated plasma membrane chloride channel is associated with regulatory volume decrease (RVD) of cells and may play an important role in control of cell proliferation. We have demonstrated that both expression of the channel and RVD capacity are actively regulated in the ...
Zuyev, Sergueï
We consider generalization of the Voronoi diagram - power diagram, constructed with respect to a Poisson processes with i.i.d. marks (weights). We give first moment of the volume distribution of a typical cell, the probability that a cell is empty, the mean length of a typical edge in the planar ...
Fabricius Katrine - - 2007
AIMS: To study if the total glial cell population in the neocortex is intact in subjects with a history of severe alcohol abuse compared to control subjects. Further, to investigate whether the cortical nerve cell nuclei and nerve cell perikarya volumes are the same in chronic alcoholic subjects as in ...
Baumann Bernd - - 2007
A procedure for the numerical calculation of photoacoustic signals is introduced. It is based on the finite element method and uses an expansion of the signal into acoustic eigenmodes of the measuring cell. Loss is included by the incorporation of quality factors. Surface and volume loss effects attributable to viscosity ...
Savage Van M - - 2007
The size and metabolic rate of cells affect processes from the molecular to the organismal level. We present a quantitative, theoretical framework for studying relationships among cell volume, cellular metabolic rate, body size, and whole-organism metabolic rate that helps reveal the feedback between these levels of organization. We use this ...
Pedrosa Rui - - 2007
The present study evaluates the presence and functional proprieties of the Na(+)-HCO(3)(-) cotransporter (NBC) in immortalized renal proximal tubular epithelial cells from spontaneously hypertensive (SHR) and normotensive Wistar-Kyoto (WKY) rats. The expected size and nucleotide sequence of a 1031-bp fragment corresponding to type 1 NBC (NBC1) was identified in both ...
Hernández Julio A - - 2007
The conservation of the cell volume within values compatible with the overall cell functions represents an ubiquitous property, shared by cells comprising the whole biological world. Water transport across membranes constitutes the main process associated to the dynamics of the cell volume, its chronic and acute regulations therefore represent crucial ...
Xue Jin - - 2007
Besides being a intracellular pH (pHi) regulator, Na+/H+ exchanger (NHE)1 has recently been postulated as a membrane scaffold that assembles protein complexes and coordinates various signaling pathways. The aim of the present study was to uncover NHE1 interactive partners and study their functional implications. NHE1 interactive partners were screened in ...
Surín Stanislav - - 2007
A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor amiloride was isolated. The Na+/H+ exchanger activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Methanogenesis rates in the mutant strain were higher than wild-type cells and resistant to the inhibitory effect of 2 ...
Endo Y - - 2007
BACKGROUND: IL-9 and IL-13 induce airway goblet cell metaplasia, which is associated with expression of a Ca(2+)-activated Cl channel, hCLCA1. OBJECTIVE: As UTP stimulates both mucin secretion and Cl ion transport via a Ca(2+)-dependent pathway, the purpose of this study is to determine whether IL-9 and IL-13 affect UTP-induced Cl ...
Ernest Nola Jean NJ Department of Neurobiology, The Civitan International Research Center, University of Alabama at Birmingham, CIRC 425, 1719 6th Avenue South, Birmingham, AL 35294, - - 2007
Mammalian cells regulate their volume to prevent unintentional changes in intracellular signaling, cell metabolism, and DNA integrity. Intentional cell volume changes occur as cells undergo proliferation, apoptosis, or cell migration. To regulate cell volume, cells use channels and transport systems to flux osmolytes across the plasma membrane followed by the ...
Silva, Paulo
Olea europaea cell cultures we have previously identified and characterized a polyol:H+ symport system whose activity was correlated with OeMaT1 (Olea europaea Mannitol Transporter) expression. Salt (100-500 mM NaCl) enhances the capacity of the transport system and the amount of OeMaT1 transcripts, whereas it strongly represses mannitol dehydrogenase activity, enzyme ...
W. Aengwanich
A study of the effects of high environmental temperatures on the packed cell volume of Thai Indigenous Chickens (TIC), Thai Indigenous Chickens Crossbred (TICC) and Broilers (BC) was made and a comparison of the effects to the 3 breeds was undertaken. One kilogram of representative males and females of each ...
Hubert Marcy D - - 2007
The goal of this study was to assess the effect of extracellular glucose and K+ ((K)o) on the intracellular osmolyte content and cell volume maintenance and regulation in a human embryonic kidney cell line (tsA201a). Cell volume maintenance was studied by isotonic (313 +/- 5 mOsm) replacement of culture media ...
Lim Christina H - - 2007
Most cells have to perform their physiological functions under a variable osmotic stress, which, because of the relatively high permeability of the plasma membrane for water, may result in frequent alterations in cell size. Intestinal epithelial cells are especially prone to changes in cell volume because of their high capacity ...
Papakonstanti Evangelia A - - 2007
Since the early days of cell volume regulation research, the role of actin cytoskeleton organization and rearrangement has attracted specific interest. Rapid modifications in actin dynamics and architecture have been described. They were shown to regulate cell volume changes, as well as regulatory volume decrease in a large variety of ...
Arduini Arduino - - 2007
L-Carnitine (LC) in the preservation medium during storage of red blood cells (RBC) can improve the mean 24-hr percent recovery in vivo and increase RBC life-span after reinfusion. The purpose of the study was to investigate the differences in the biochemical properties of RBCs stored in the presence or absence ...
Mao Jianwen - - 2007
Cell migration is crucial for tumor metastasis. Membrane ion channels may play a major role in tumor cell migration because the cells must undergo changes in shape and volume during migration. In the present study, we used the transwell migration assay, an in vitro model for cell migration, and the ...
Gardner David K - - 2007
Because metabolism is fundamental to the successful functioning of a cell, perturbations in metabolism can signal compromised viability. For the assessment of viability, analyses not only must be sensitive enough to be employed at the single cell level, but also noninvasive. Ultramicrofluorescence is a technique that fulfills both criteria. By ...
Graves Steven W - - 2007
Preeclampsia is a disease characterized by hypertension and proteinuria but can manifest many abnormalities. Some of the best documented alterations involve changes in the handling of sodium ion both on the systemic and on the cellular level. There is broad agreement that the components of the renin-angiotensin-aldosterone pathway are markedly ...
Mathern Gary W - - 2007
This study compared MRI cerebral volumes and Neuronal-Nuclei (NeuN) cell densities in pediatric epilepsy surgery patients with cortical dysplasia (CD; n = 25) and hemimegalencephaly (HME; n = 14). Our purpose was to deduce possible mechanisms of pathogenesis and epileptogenesis based on an understanding of normal developmental corticoneurogenesis. We used ...
Miguel Amparo - - 2007
The automated hematology cell analyzer Coulter LH 750 (Beckman Coulter, Brea, CA, USA) uses a combination of 3 measurements-volume, conductivity, and scatter-to identify cells, but it could take advantage of these parameters to evaluate their morphologic changes. The neutrophil mean cell volume (MNEV), mean cell conductivity (MNEC), and mean cell ...
Schneider Juliane - - 2007
Hallmarks of apoptosis include cell shrinkage, which is at least partially due to cellular K(+) loss. The decline of cellular K(+) concentration has been suggested to participate in the triggering of apoptosis. Suicidal erythrocyte death or eryptosis is triggered by increased cytosolic Ca(2+) activity leading to activation of Ca(2+)-sensitive K(+) ...
Lang Florian - - 2007
Alterations of cell volume are key events during both cell proliferation and apoptotic cell death. Cell proliferation eventually requires an increase of cell volume, and apoptosis is typically paralleled by cell shrinkage. Alterations of cell volume require the participation of ion transport across the cell membrane, including appropriate activity of ...
Stock Christian - - 2007
Extracellular pH and the Na(+)/H(+) exchanger (NHE1) modulate tumor cell migration. Yet, the pH nanoenvironment at the outer surface of the cell membrane (pH(em)) where cell/matrix interaction occurs and matrix metalloproteinases work was never measured. We present a method to measure this pH nanoenvironment using proton-sensitive dyes to label the ...
< 1 2 3 4 5 6 7 8 9 10 >