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Emanuele Enzo E Living Research s.a.s., I-27038 Robbio, Pavia, - - 2014
Trehalose, a naturally occurring non-reducing disaccharide, is known to act as a major protein stabilizer that can reduce ultraviolet B (UVB)-induced corneal damage when topically applied to the eye. However, due to the low skin permeability of trehalose, which makes the development of topical formulations difficult, its use as a ...
Wang Lin L School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowlong Tong, Kowloon, Hong Kong, - - 2014
Ginsenoside Rb1 (Rb1) is the most predominant ginsenoside isolated from the roots of ginseng (Panax ginseng C. A. Meyer). This compound is active in various human biological pathways that are involved in human collagen synthesis and inhibition of cell apoptosis. In this study, the skin-whitening effects of Rb1 were investigated ...
Taha Mariam M Canadian Blood Services, Ottawa, Ontario, - - 2014
A one-step skin disinfection method containing 2% chlorhexidine-gluconate (CHG) and 70% isopropyl alcohol (IPA) is currently used by blood suppliers worldwide. Reports of bacterially contaminated platelet concentrates (PCs) indicate that skin disinfection is not fully effective. Approximately 20% of skin microflora exist as surface-attached aggregates (biofilms), known for displaying increased ...
van Drongelen Vincent V Leiden University Medical Centre, Dermatology, Einthovenweg 20, Leiden, Netherlands, 2300 RC, +31-71-5269365, Leiden Universtity, Department of Drug Delivery Technology, Einsteinweg 55, Leiden, Netherlands, 2333 CC ; - - 2014
Human skin equivalents (HSEs) can be a valuable tool to study aspects of human skin, including the skin barrier, or to perform chemical or toxicological screenings. HSEs are three-dimensional skin models that are usually established using primary keratinocytes and closely mimic human skin. The use of primary keratinocytes has several ...
Tewari-Singh Neera N Department of Pharmaceutical - - 2014
Our previous studies and other published reports with the chemical warfare agent sulfur mustard (SM) and its analog 2-chloroethyl ethyl sulfide (CEES) have indicated a role of oxidative stress in skin injuries caused by these vesicating agents. We examined the effects of the catalytic antioxidant AEOL 10150 in attenuation of ...
Pérez-Sánchez A A Skin Research Platform (SRP), Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Avenida de la Universidad s/n, E-03202 Elche, Alicante, - - 2014
Ultraviolet radiation absorbed by the epidermis is the major cause of various cutaneous disorders, including photoaging and skin cancers. Although topical sunscreens may offer proper skin protection, dietary plant compounds may significantly contribute to lifelong protection of skin health, especially when unconsciously sun UV exposed. A combination of rosemary and ...
Dornmair Klaus K Institute of Clinical Neuroimmunology, University Hospital Grosshadern, LMU Munich, D-81377, Munich, Germany; Munich Cluster for Systems Neurology - - 2014
Tissue-invasive T cells are observed in many inflammatory dermatological diseases, but in most cases it is not known how they were attracted, what they might recognize, and to which extent they are activated. Answering these questions is surely essential for understanding pathogeneses of the diseases. Smith et al. (1) showed that ...
Cholet Céline - - 2014
Pulsed electric field (PEF) treatment is an emerging technology that is arousing increasing interest in vinification processes for its ability to enhance polyphenol-extraction performance. The aim of this study was to investigate the effects of PEF treatment on grape-skin histo-cytological structures and on the organization of skin cell-wall polysaccharides and ...
Chevalier Christine C Unité de Biologie moléculaire du Développement, Institut Pasteur, 25, rue du Docteur Roux, 75724, Paris Cedex 15, France, - - 2014
To study the cell behavior during morphogenesis of mouse surface ectoderm, skin, and hair follicles, we (1-3) have developed a new method to temporally induce clones that is based on a tamoxifen-dependent Cre recombinase. The classical protocol consisting in dissolving 4-hydroxy-tamoxifen or tamoxifen in corn oil to perform intraperitoneal (ip) ...
Kozdęba Marcin M Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, - - 2014
The occurrence of cyanobacterial toxic peptides, including microcystins (MCs), is an emerging health issue due to the eutrophication of water bodies. MCs have a strong influence on human cells, predominantly hepatocytes, however, toxicity was also observed in kidney, lung and dermal skin cells. Skin as the most external barrier of ...
Bindon Keren A - - 2014
The acetone-extractable (70% v/v) skin tannin content of Vitis vinifera L. cv. Cabernet Sauvignon grapes was found to increase during late-stage ripening. Conversely, skin tannin content determined following ethanol extraction (10%, 20% and 50% v/v) did not consistently reflect this trend. The results indicated that a fraction of tannin became ...
Choi Sang Yoon SY Korea Food Research Institute, Seongnam 463-746, Korea. - - 2014
In this study, the inhibitory effect of corn silk on melanin production was evaluated. This study was performed to investigate the inhibitory effect of corn silk on melanin production in Melan-A cells by measuring melanin production and protein expression. The corn silk extract applied on Melan-A cells at a concentration ...
Ambler Carrie A - - 2013
Artificial epidermis can be reconstituted in vitro by seeding primary epidermal cells (keratinocytes) onto a supportive substrate and then growing the developing skin equivalent at the air-liquid interface. In vitro skin models are widely used to study skin biology and for industrial drug and cosmetic testing. Here, we describe updated ...
Sharma Kavita - - 2013
Tissue engineering has progressed in delivering laboratory-expanded keratinocytes to the clinic; however the production of a suitable alternative to a skin graft, containing both epidermis and dermis still remains a challenge. To develop a one-step approach to wound reconstruction using finely minced split thickness skin and a biodegradable synthetic dermal ...
Li Shi-Ying SY Department of Forensic Pathology, Institute of Forensic Science, Ministry of Justice, 200063, Shanghai, P.R. China; Department of Forensic Medicine, Shanghai Medical College, Fudan University, 200032, Shanghai, P.R. - - 2014
This technical note describes a method for distinguishing normal skin tissue samples from those electrically injured by Fourier transform infrared microspectroscopy (FTIR MSP). Furthermore, the infrared spectral features of electrically injured cells and tissues were evaluated to identify molecular changes in epidermal cells. In the present study, 20 human hand ...
Lemper M - - 2013
Histone deacetylase inhibitors (HDACi), a relatively new group of epigenetic agents, are being investigated as powerful chemotherapeutics because of their antiproliferative and prodifferentiation effects both in vitro and in vivo, in various tumor cell lines. Only little is known with respect to their effects on normal cells. Yet, to understand ...
Pleitez Miguel A - - 2013
The application of a novel open, windowless cell for the photoacoustic infrared spectroscopy of human skin is described. This windowless cavity is tuned for optimum performance in the ultrasound range between 50 and 60 kHz. In combination with an external cavity tunable quantum cascade laser emitting in the range from ...
Brauchle Eva - - 2013
Tissue-engineered skin equivalents based on primary isolated fibroblasts and keratinocytes have been shown to be useful tools for functional in vitro tests, including toxicological screenings and drug development. In this study, a commercially available squamous cell carcinoma (SCC) cell line SCC-25 was introduced into epidermal and full-thickness skin equivalents to generate ...
Morhenn V B - - 2013
Psoriasis shares many features with wound healing, a process that involves switching keratinocytes from growth to differentiation. Ca2+ is known to regulate this process. The N-methyl-d-aspartate receptor (NMDAR), an ionotropic glutamate receptor found on keratinocytes, is expressed abnormally in psoriasis in vivo. The goals of this study are to determine ...
Morita Tomotake - - 2013
Mannosylerythritol lipids (MELs) are glycolipid biosurfactants abundantly produced by different basidiomycetous yeasts such as Pseudozyma, and show not only excellent interfacial properties but also versatile biochemical actions. These features of MELs make their application in new technology areas possible. Recently, the structural and functional variety of MELs was considerably expanded ...
Schmidt Barbara A BA Yale University, Department of Molecular, Cellular and Developmental Biology, New Haven, CT 06520, - - 2013
Acute wound healing in the skin involves the communication of multiple cell types to coordinate keratinocyte and fibroblast proliferation and migration for epidermal and dermal repair. Many studies have focused on the interplay between hematopoietic cells, keratinocytes and fibroblasts during skin wound healing, yet the possible roles for other cell ...
Dixon Katie M - - 2013
Exposure to sunlight is the major cause of skin cancer. Ultraviolet radiation (UV) from the sun causes damage to DNA by direct absorption and can cause skin cell death. UV also causes production of reactive oxygen species that may interact with DNA to indirectly cause oxidative DNA damage. UV increases ...
Hintsala Hanna-Riikka - - 2013
Claudins are tight junction proteins regulating the paracellular permeability of cell layers. We investigated the expression of claudins 1, 2, 3, 4, 5 and 7 in a sample set consisting of a total of 93 cases representing normal skin, actinic keratoses and squamous cell carcinomas of the skin. There were ...
Johnston Adam P W AP Program in Developmental and Stem Cell Biology, Hospital for Sick Children, Toronto, ON M5G 1L7, - - 2013
Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precursor cells (NCPCs) around the ...
Jean Jessica - - 2012
Current knowledge suggests that uninvolved psoriatic skin could demonstrate characteristics associated with both normal and involved psoriatic skins. However, the triggering factor allowing the conversion of uninvolved skin into a psoriatic plaque is not fully understood. To counter this lack of information, we decided to develop pathological skin substitutes produced ...
Korkina L G - - 2012
As the incidence of skin tumours has been steadily growing, there is an urgent need for the preventive measures as well as in the improved therapeutic approaches. In the last two decades, natural plant derived polyphenols (PPs, resveratrol, silibinin, green tea polyphenols, flavonoids, anthocyanins, etc.) have been drawing particular interest ...
Schmidt Barbara - - 2012
Here, we explore the established and potential roles for intradermal adipose tissue in communication with hair follicle biology. The hair follicle delves deep into the rich dermal macroenvironment as it grows to maturity where it is surrounded by large lipid-filled adipocytes. Intradermal adipocytes regenerate with faster kinetics than other adipose ...
Herman A - - 2012
Caffeine is being increasingly used in cosmetics due to its high biological activity and ability to penetrate the skin barrier. This alkaloid is frequently used as a hydrophilic model substance in human and animal skin penetration as well as different synthetic membrane using Franz diffusion cell experiments. The commercially available ...
Nie J - - 2012
Skin homeostasis is maintained by controlling the balance between proliferation and differentiation of epidermal stem cells. The microenvironment, including extrinsic stresses, growth factors, soluble molecules, cell-ECM and cell-cell communications, plays an important role in cell fate determination in vivo and in vitro. In response to external signals, keratinocytes cooperate with ...
Lee Jeong-Hyun - - 2012
Wound healing proceeds through a complex collaborative process involving many types of cells. Keratinocytes and fibroblasts of epidermal and dermal layers of the skin play prominent roles in this process. Asiaticoside, an active component of Centella asiatica, is known for beneficial effects on keloid and hypertrophic scar. However, the effects ...
Zhang Xian-Hua - - 2012
Resveratrol (3,4,5-trihydroxy-trans-stilbene), a phytoalexin found in grape skin, grape products, and peanuts as well as red wine, has been reported to have various biological and pharmacological properties. The purpose of this study was to investigate the anti-obesity effect of resveratrol-amplified grape skin extracts on adipocytes. The anti-obesity effects of grape ...
Inami Yoshihiro - - 2012
The primary function of surfactants is to remove dirt, exfoliated corneum cells, and microorganisms from the skin. However, the use of toiletries such as soaps and shampoos containing surfactants may cause adverse effects such as cutaneous irritation, dryness, and itching. Recently, skin pathologies, including dry skin, rough skin, and sensitive ...
Rotondo Fabio - - 2012
The aim of this study was to immunohistochemically localize ghrelin in autopsy-obtained, nontumoral human pituitaries. Double immunostaining was also undertaken to determine the pituitary cell type expressing both adenohypophysial hormones and ghrelin. Results showed that ghrelin is present in the adenohypophysis, its immunoexpression being cytoplasmic, weak-to-moderate, and localized to a ...
Berman Brian - - 2012
Actinic keratosis is a common disease in older, fair-skinned people, and is a consequence of cumulative ultraviolet exposure. It is part of a disease continuum in photodamaged skin that may lead to invasive squamous cell carcinoma. Treatment options frequently used include cryosurgery and topical pharmacologic agents, which are examples of ...
Seet Wan Tai - - 2012
Skin plays an important role in defense against infection and other harmful biological agents. Due to its fragile structure, skin can be easily damaged by heat, chemicals, traumatic injuries and diseases. An autologous bilayered human skin equivalent, MyDerm™, was engineered to provide a living skin substitute to treat critical skin ...
Cha Kwang Hyun - - 2011
<i>Chlorella</i> is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from <i>Chlorella vulgaris</i> using a coupled <i>in vitro</i> digestion and human intestinal Caco-2 cell model. Lutein bioavailability was low and approximately 75% of total <i>C. vulgaris</i> lutein ...
Kramer Susanne - - 2011
Infection of certain cell types by HIV results in formation of syncytia. This process can be blocked by antibodies or compounds that prevent interaction of viral envelope protein with host cell receptors. Here the authors describe an automated imaging-based assay for inhibitors of cell-cell fusion mediated by interaction of HIV ...
Bronshtein Tomer - - 2011
Anti-retroviral-therapies against HIV/AIDS focus on inhibiting viral growth and may slow AIDS progression, but not cure the disease. Here we describe an approach to treat HIV as a cellular pathology by targeting cell derived liposomes against HIV-infected cells. Cell-derived-liposomes were prepared from the cytoplasmatic membranes of cells expressing CCR5, the ...
Chen Ping - - 2011
In animal models of HIV-associated nephropathy, the expression of HIV regulatory genes in epithelial cells is sufficient to cause disease, but how the CD4-negative epithelial cells come to express HIV genes is unknown. Here, we co-cultured T cells infected with fluorescently tagged HIV with renal tubular epithelial cells and observed ...
Treuer Robin R School of Life Sciences, Arizona State University, Tempe, Arizona, - - 2011
Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to ...
Yang Sung Ho - - 2011
The individual encapsulation of living cells has a great impact on the area of cell-based sensors and devices as well as fundamental studies in cell biology. In this work, living yeast cells were individually encapsulated with functionalizable, artificial polydopamine shells, inspired by an adhesive protein in mussels. Yeast cells maintained ...
Turgeon Robert - - 2011
Phloem loading is the process by which photoassimilates synthesized in the mesophyll cells of leaves enter the sieve elements and companion cells of minor veins in preparation for long distance transport to sink organs. Three loading strategies have been described: active loading from the apoplast, passive loading via the symplast, ...
Moss Darren M - - 2011
The identification of transporters of the HIV integrase inhibitor raltegravir could be a factor in an understanding of the pharmacokinetic-pharmacodynamic relationship and reported drug interactions of raltegravir. Here we determined whether raltegravir was a substrate for ABCB1 or the influx transporters SLCO1A2, SLCO1B1, SLCO1B3, SLC22A1, SLC22A6, SLC10A1, SLC15A1, and SLC15A2. ...
Matsubara Yumiko - - 2010
Platelets are produced from megakaryocytes (MKs), although the pathway leading from stem cells to MK lineages are not yet fully understood. Recently, we reported to obtain abundant MKs and platelets from human subcutaneous adipose tissues. Adipose tissues contain various cell types, most of which are lineage cells from mesenchymal or ...
Sakwe Amos M AM Department of Biochemistry and Cancer Biology, Meharry Medical College, Nashville, Tennessee 37208, - - 2010
The identity of the cell adhesive factors in fetal bovine serum, commonly used to supplement growth media, remains a mystery due to the plethora of serum proteins. In the present analyses, we showed that fetuin-A, whose function in cellular attachment in tissue culture has been debated for many years, is ...
Zhang Peiqing - - 2010
Significant efforts have been made to improve the sialylation of recombinant glycoproteins with the aim of extending their in vivo circulation time. Here, we report a systematic functional analysis of 31 N-glycosylation-related genes on sialylation of recombinant EPO in six cell lines. BHK and CHO cells were found to sialylate ...
Fan Yuying - - 2010
We tested the effects of ginseng pectin prepared by enzymatic hydrolysis of ginseng polysaccharides on cell migration. Ginseng pectin impaired the migration of L-929 cells and reduced their migration speed by up to 50% of control in the presence or absence of serum, suggesting it worked on both serum-dependent and ...
Ranzato Elia - - 2010
HMGb1 is a nuclear protein playing a role in DNA architecture and transcription. This protein has also been shown to function as a cytokine and to stimulate keratinocyte scratch wound healing. Due to the importance of finding new wound healing molecules, we have studied the effects of HMGb1 on fibroblasts, ...
Takai Noriyuki - - 2010
β-hydroxyisovalerylshikonin (β-HIVS), a compound isolated from the traditional asian medicinal herb Lithospermum radix, is an ATP non-competitive inhibitor of protein-tyrosine kinases such as v-Src and EGFR, and has been shown to induce apoptosis in several human tumor cell lines. We investigated the effect of β-HIVS in the choriocarcinoma cell line, ...
Liu Jinyao - - 2010
Polymeric micelles are recognized as very promising nanocarriers for small-molecule drugs, DNA, and proteins delivery. Polyphosphates are an important class of eminent biomaterials and widely used in biomedicine due to their good biocompatibility, biodegradability, and flexibility in adjusting the pendant structures. Here, some full-polyphosphate nanocarriers have been constructed successfully through ...
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