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Bodily Jason M JM Department of Microbiology and Immunology, Louisiana State University, Health Sciences Center, 1501 Kings Highway, Shreveport, LA, USA, - - 2015
Human papillomaviruses are the causative agents of several cancers, but only a minority of HPV infections progress to malignancy. In order to better understand HPV biology during the normal, differentiation-dependent life cycle, a cell culture model that maintains the complete episomal genome and permits host cell differentiation is critical. Furthermore, ...
Wedlich-Söldner Roland R Institute of Cell Dynamics and Imaging and Cells-in-Motion Cluster of Excellence (EXC 1003 - CiM), University of Muenster, Von Esmarchstrasse 56, 48149 Muenster, Germany. Electronic address: - - 2014
Cell polarity establishment has been studied in great detail, but much less is known about mechanisms that prevent polarization. Reporting recently in Cell, Meitinger et al. (2014) identify an elaborate mechanism in yeast cells that efficiently inhibits Cdc42 activation in cytokinesis remnants. Failure of this "anti-polarization" memory increases replicative aging.
de Souza Tereza Pereira TP Institut für Pharmazie, Friedrich Schiller Universität Jena, Lessingstrasse 8, 07743, Jena, - - 2014
One of the main open questions in origin of life research focuses on the formation, by self-organization, of primitive cells composed by macromolecular compounds enclosed within a semi-permeable membrane. A successful experimental strategy for studying the emergence and the properties of primitive cells relies on a synthetic biology approach, consisting ...
Costa Barbara B Department of Pharmacy, University of Pisa, via Bonanno, 6-56126, Pisa, Italy, - - 2014
Ligands addressed to the mitochondrial Translocator Protein (TSPO) have been suggested as cell death/life and steroidogenesis modulators. Thus, TSPO ligands have been proposed as drug candidates in several diseases; nevertheless, a correlation between their binding affinity and in vitro efficacy has not been demonstrated yet, questioning the specificity of the ...
Blagosklonny Mikhail V MV a Cell Stress Biology , Roswell Park Cancer Institute , Buffalo , NY 14263 - - 2014
Abstract Cellular senescence happens in two steps: cell cycle arrest followed, or sometimes preceded, by gerogenic conversion (geroconversion). Geroconvesrion is a form of growth, a futile growth during cell cycle arrest. It converts reversible arrest to irreversible senescence. Geroconversion is driven by growth-promoting, mitogen-/nutrient-sensing pathways such as mTOR. Geroconversion leads ...
Hammerschmidt Katrin K New Zealand Institute for Advanced Study and Allan Wilson Centre for Molecular Ecology &Evolution, Massey University, Auckland 0745, New - - 2014
Cooperation is central to the emergence of multicellular life; however, the means by which the earliest collectives (groups of cells) maintained integrity in the face of destructive cheating types is unclear. One idea posits cheats as a primitive germ line in a life cycle that facilitates collective reproduction. Here we ...
Fernandes P P 1] iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal [2] Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, - - 2014
Helper-dependent adenovirus vectors (HDVs) are safe and efficient tools for gene transfer with high cloning capacity. However, the multiple amplification steps needed to produce HDVs hamper a robust production process and in turn the availability of high-quality vectors. To understand the factors behind the low productivity, we analyzed the progression ...
Salam Jaseetha Abdul JA Bioremediation Lab, Environmental Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, - - 2014
The objective of this study was to evaluate the effect of an embedded bio-nano hybrid system using nanoscale zinc oxide (n-ZnO) and lindane-degrading yeast Candida VITJzN04 for lindane degradation. Nano-embedding of the yeast was done with chemically synthesized n-ZnO particles (50 mg/mL) and was visualized by atomic force microscope (AFM) and ...
Tate Matthew C MC Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California - San Francisco, San Francisco, CA, 94143; Department of Neurological Surgery, University of California - San Francisco, San Francisco, CA, - - 2014
Despite its critical importance to global brain function, the postnatal development of the human pons remains poorly understood. In the present study, we first performed MRI-based morphometric analyses of the postnatal human pons (0-18 years;n=6-14/timepoint). Pons volume increased 6-fold from birth to 5 years, followed by continued slower growth throughout ...
Pasco Matthieu Y MY Sophia Agrobiotech Institute, UMR INRA/CNRS/Nice-Sophia Antipolis University, Sophia Antipolis Cedex, - - 2014
The digestive tract is subjected to many aggressions throughout animal life. Since disruptions of gut physiology impact on animal fitness and survival, maintenance of gut integrity and functionality is essential for the individual. Over the last 40 years, research on rodents has aimed at understanding how cellular homeostasis of the ...
Baek Soonbong - - 2014
Life on earth is exposed to natural electromagnetic fields and it is generally accepted that electromagnetic fields (EMF) may exert a variety of effects on biological systems. Particularly, extremely low frequency electromagnetic fields (EL-EMFs) affect biological functions such cell development & differentiation, however, the fundamental roles by which EMF influences ...
Ferris Trevor J TJ University of Kent, School of Physical Sciences, Canterbury CT2 7NH, UK. - - 2014
The increasing availability of the long half-life positron emitter Zr-89 (half life 78.4 h) suggests that it is a strong candidate for cell labelling and hence cell tracking using positron emission tomography. The aim was to produce a range of neutral ZrL4 lipophilic complexes for cell labelling which could be ...
Fisichella Matthieu - - 2014
Engineered nanomaterials may release nanosized residues, by degradation, throughout their life cycle. These residues may be a threat for living organisms. They may be ingested by humans through food and water. Although the toxicity of pristine CeO2 nanoparticles (NPs) has been documented, there is a lack of studies on manufactured ...
Ramalhete Cátia C iMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, - - 2014
Sixteen triterpenoids (1-16), previously isolated from the aerial parts of the African medicinal plant Momordica balsamina or obtained by derivatization, were evaluated for their activity against liver stages of Plasmodium berghei, measuring the luminescence intensity in Huh-7 cells infected with a firefly luciferase-expressing P. berghei line, PbGFP-Luccon. Toxicity of compounds ...
Schuck Desirée C DC Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Cidade Universitária, 05508-900 São Paulo, SP, Brazil; Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Cidade Universitária, 05508-900 São Paulo, SP, - - 2014
Discovering the mechanisms by which cell signaling controls the cell cycle of the human malaria parasite Plasmodium falciparum is fundamental to designing more effective antimalarials. To better understand the impacts of melatonin structure and function on the cell cycle of P. falciparum, we have synthesized two families of structurally-related melatonin compounds ...
Mohapatra S S Senior Research Associate, Department of Microbiology, AIIMS, New Delhi-110029, India; phone: +91 9810726220; e mail: - - 2014
Goals Automated flowcytometric analysers are used to analyse complete blood count (CBC) in most patients. They differentiate WBC depending on their size, internal granularity, and nuclear content by producing various scattergrams. In malaria, haemozoin pigment-containing cells (parasitized RBC and phagocytic cells) are able to depolarize the laser beam and therefore ...
Alves-Junior Eduardo R ER Julio Müller Hospital, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil; Biomedicine Department, Univag University Centre, Varzea Grande, Mato Grosso, - - 2014
Quantification of parasite density is an important component in the diagnosis of malaria infection. The accuracy of this estimation varies according to the method used. The aim of this study was to assess the agreement between the parasite density values obtained with the assumed value of 8,000 cells/μL and the ...
Davanço Marcelo Gomes MG Departamento de Princípios Ativos Naturais e Toxicologia, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista - UNESP, Araraquara, São Paulo, - - 2014
Plasmodium vivax is the most prevalent of the five species causing malaria in humans. The current available treatment for P. vivax malaria is limited and unsatisfactory due to at least two drawbacks: the undesirable side effects of primaquine (PQ) and drug resistance to chloroquine. Phenylalanine-alanine-PQ (Phe-Ala-PQ) is a PQ prodrug ...
Sutphin George L GL Department of Pathology, University of Washington, Health Science Building D-514, 357470, Seattle, WA, 98195-7470, - - 2014
Identifying and characterizing the factors that modulate longevity is central to understanding the basic mechanisms of aging. Among model organisms used for research related to aging, the budding yeast has proven to be an important system for defining pathways that influence life span. Replicative life span is defined by the ...
Dolfi Luca L Scuola Normale Superiore, Pisa, - - 2014
Annual killifishes inhabit temporary ponds and their embryos survive the dry season encased in the mud by entering diapause, a process that arrests embryonic development during hostile conditions. Annual killifishes are present within three clades distributed in Africa (one East and one West of the Dahomey gap) and South America. ...
Chanyi Ryan M RM Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, - - 2014
Bdellovibrio bacteriovorus, as an obligate predator of Gram-negative bacteria, requires contact with the surface of a prey cell in order to initiate the life cycle. After attachment, the predator penetrates the prey cell outer membrane and enters the periplasmic space. Attack phase cells of B. bacteriovorus have polar Type IV ...
Zhu Yushan Y State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin, - - 2014
It is essential for a cell to maintain a proper mitochondrial quality and quantity for normal cellular functions. Damaged or unwanted mitochondria can be selectively removed through mitophagy. Mitophagy research has attracted great attention from life sciences and biomedical fields, it is thus important for the community to properly measure ...
Tremp Annie Z AZ Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, WC1E 7HT, - - 2013
A shared feature of the motile stages (zoites) of malaria parasites is a cortical cytoskeletal structure termed subpellicular network (SPN), thought to define and maintain cell shape. Plasmodium alveolins comprise structural components of the SPN, and alveolin gene knockout causes morphological abnormalities that coincide with markedly reduced tensile strength of ...
Niemand Jandeli J Departments of Biochemistry, University of Pretoria, Hatfield, South - - 2013
Anthracene-polyamine conjugates inhibit the in vitro proliferation of the intraerythrocytic human malaria parasite Plasmodium falciparum, with 50% inhibitory concentrations (IC50s) in the nM to μM range. The compounds are taken up into the intraerythrocytic parasite, where they arrest the parasite cell cycle. Both the anthracene and polyamine components of the ...
Désidéri-Vaillant C - - 2012
The high frequency of the gene for sickle-cell hemoglobin in malaria-endemic regions is believed to be due to an advantage in surviving malaria. We report the case of a woman with both malaria and hemoglobin S.
Gupta Narendra Kumar NK Department of Pathology, ESIC Model Hospital, Nandanagar, Indore, MP - - 2014
Sickle cell disease is the prototype of hereditary hemoglobinopathies, characterized by the production of structurally abnormal hemoglobin. Sickle cell anemia results from a point mutation that leads to substitution of valine for glutamic acid at the sixth position of the β globin chain. We report a young male admitted with ...
Zhao Nan - - 2012
The apparently higher crash risk among individuals who use cell phones while driving may be due both to the direct interference of cell phone use with the driving task and tendencies to engage in risky driving behaviors independent of cell phone use. Measurements of actual highway driving performance, self-reported aberrant ...
Elbeltagy Maha - - 2012
Chemotherapy has been reported to produce cognitive impairments in a significant number of cancer patients. These deficits frequently involve aspects of spatial or declarative memory which can persist for up to several years after completion of the treatment. We have recently shown that 5Fluorouracil (5-FU), a commonly used chemotherapy drug, ...
Kim Deok-Ho - - 2012
The architecture of the extracellular matrix (ECM) directs cell behavior by providing spatial and mechanical cues to which cells respond. In addition to soluble chemical factors, physical interactions between the cell and ECM regulate primary cell processes, including differentiation, migration, and proliferation. Advances in microtechnology and, more recently, nanotechnology provide ...
Ono Masafumi - - 2012
See article in J. Gastroenterol. Hepatol. 2012; 27: 789-796.
Haining W Nicholas WN Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA. - - 2012
Memory T cells are heterogeneous in phenotype and function. In this issue of Immunity, Newell et al. (2012) use a new flow cytometry platform to show that the functional heterogeneity of the human T cell compartment is even greater than previously thought.
Kim Tae Rim - - 2012
Chemo- or radiation-resistance in tumors caused by hypoxia often undermines efficacy of cancer therapy. Thus, therapies that overcome cellular resistance during hypoxia are necessary. SM22α is an actin-binding protein found in smooth muscle, fibroblasts, and some epithelium. We demonstrate that SM22α is induced in A549 non-small cell lung carcinoma cells ...
Edin Nina Jeppesen NJ Department of Physics, University of Oslo, Norway. - - 2012
To investigate the effect of cycling hypoxia on low dose hyper-radiosensitivity (HRS). Human breast tumor T-47D cells were grown in a hypoxia workstation operated at 4% O(2) for 3-6 weeks and the pericellular oxygen concentration was recorded every 20 minutes. The presence of HRS in response to subsequent challenge irradiation ...
Yamasaki Mutsushi - - 2012
PURPOSE: Tumor hypoxia is a common feature of any cancer, including prostate cancer (CaP), and associated with tumor cell aggressiveness. Although some reports are available on acute hypoxia-response in CaP cells aggressiveness, little is known about chronic hypoxia-response. We investigated the effects of chronic hypoxia on human CaP cells. MATERIALS ...
Hofstetter Christoph P - - 2012
The hypoxic microenvironment of glioblastoma multiforme (GBM) is thought to increase resistance to cancer therapies. Recent evidence suggests that hypoxia induces protein phosphatase 2A (PP2A), a regulator of cell cycle and cell death. The effects of PP2A on GBM tumor cell proliferation and survival during hypoxic conditions have not been ...
Dong X L - - 2011
Hypoxia, frequently found in the center of solid tumors, may lead to enhance the production of key factor in cell survival, invasion, angiogenesis and loss of apoptosis. The low oxygen tension in hypoxic tumors is also known to interfere with the efficacy of chemotherapy, but the underlying mechanisms are not ...
Jiang Yi-Fan - - 2011
Hypoxia is an important physiological process which ensures corpus luteum (CL) formation and development, thus playing an important role in steroidogenesis. Recent studies have shown that CL develops in an analogous to tumorigenesis by accumulation of hypoxia-inducible factor-1 alpha subunit (HIF1A) in response to hypoxia. To investigate the relationship among ...
Chanyshev B - - 2011
The hypothesis of the present study is that cardiomyocytes subjected to prolonged ischemia, may release survival factors that will protect new cardiac cells from ischemic stress. We exposed neonatal rat cardiomyocyte primary cultures to hypoxia, collected the supernatant, treated intact cardiac cells by this posthypoxic supernatant, and exposed them to ...
Assaly Rana - - 2011
Reactive oxygen species production is necessary to induce cell death following hypoxia/reoxygenation but the effect of reactive oxygen species produced during hypoxia on mitochondrial permeability transition pore (mPTP) opening and cell death is not established. Here we designed a model of hypoxia/reoxygenation in isolated cardiomyocytes measuring simultaneously reactive oxygen species ...
Meng Fanying F Threshold Pharmaceuticals, 170 Harbor Way, Suite 300, South San Francisco, CA 94080, USA. - - 2012
TH-302 is a 2-nitroimidazole triggered hypoxia-activated prodrug (HAP) of bromo-isophosphoramide mustard currently undergoing clinical evaluation. Here, we describe broad-spectrum activity, hypoxia-selective activation, and mechanism of action of TH-302. The concentration and time dependence of TH-302 activation was examined as a function of oxygen concentration, with reference to the prototypic HAP ...
Flügel Daniela - - 2012
The hypoxia-inducible transcription factor-1α (HIF-1α) is a major regulator of angiogenesis, carcinogenesis, and various processes by which cells adapt to hypoxic conditions. Therefore, the identification of critical players regulating HIF-1α is not only important for the understanding of angiogenesis and different cancer phenotypes, but also for unraveling new therapeutic options. ...
Cui L Y - - 2011
Neutrophil gelatinase-associated lipocalin (NAGL) was first extracted from neutrophil granules. Our previous study showed that the expression of NGAL mRNA and protein can be induced by hypoxia/reoxygenation. This study was designed to investigate the relationship between NGAL and hypoxia/reoxygenation injury pathologies in HK-2 cells. The effect of NGAL on the ...
Wanka C - - 2011
P53 has an important role in the processing of starvation signals. P53-dependent molecular mediators of the Warburg effect reduce glucose consumption and promote mitochondrial function. We therefore hypothesized that the retention of wild-type p53 characteristic of primary glioblastomas limits metabolic demands induced by deregulated signal transduction in the presence of ...
Metallo Christian M CM Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, - - 2012
Acetyl coenzyme A (AcCoA) is the central biosynthetic precursor for fatty-acid synthesis and protein acetylation. In the conventional view of mammalian cell metabolism, AcCoA is primarily generated from glucose-derived pyruvate through the citrate shuttle and ATP citrate lyase in the cytosol. However, proliferating cells that exhibit aerobic glycolysis and those ...
Tan Can - - 2011
The ubiquitin E3 ligase CUL4A plays important roles in diverse cellular processes including carcinogenesis and proliferation. It has been reported that the expression of CUL4A can be induced by hypoxic-ischemic injury. However, the effect of elevated expression of CUL4A on hypoxia-reoxygenation injury is currently unclear. In this study, human CUL4A ...
Szoleczky Petra - - 2011
Acute tubular necrosis is a clinical problem that lacks specific therapy and is characterized by high mortality rate. The ischemic renal injury affects the proximal tubule cells causing dysfunction and cell death after severe hypoperfusion. We utilized a cell-based screening approach in a hypoxia-reoxygenation model of tubular injury to search ...
Kaida Atsushi - - 2011
Fluorescent proteins are widely used for the direct visualization of events such as gene expression and subcellular localization in mammalian cells. It is well established that oxygen is required for formation of functional chromophore; however, the effect of hypoxia on fluorescence emission has rarely been studied. For this purpose, under ...
Jeong Jae-Kyo - - 2011
The human prion protein fragment, PrP (106-126), may contain a majority of the pathological features associated with the infectious scrapie isoform of PrP, known as PrP(Sc). Based on our previous findings that hypoxia protects neuronal cells from PrP (106-126)-induced apoptosis and increases cellular prion protein (PrP(C)) expression, we hypothesized that ...
Naldini Antonella - - 2011
The tumour microenvironment is characterized by a poor circulation which results in selection of neoplastic cells that can grow or survive under hypoxic conditions. The relationship between hypoxia and histone deacetylase (HDAC) inhibitors has been previously established. In this work we evaluated the effects of novel HDAC inhibitors (the natural ...
Burrows Natalie N Hypoxia and Therapeutics Group, School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United - - 2011
Phosphoinositide 3-kinase (PI3K) regulates the transcription factor hypoxia-inducible factor-1 (HIF-1) in thyroid carcinoma cells. Both pathways are associated with aggressive phenotype in thyroid carcinomas. Our objective was to assess the effects of the clinical PI3K inhibitor GDC-0941 and genetic inhibition of PI3K and HIF on metastatic behavior of thyroid carcinoma ...
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