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Kim Eugene E - - 2014
Cardiac Purkinje cells are important triggers of ventricular arrhythmias associated with heritable and acquired syndromes; however, the mechanisms responsible for this proarrhythmic behavior are incompletely understood. Here, through transcriptional profiling of genetically labeled cardiomyocytes, we identified expression of Purkinje cell protein-4 (Pcp4), a putative regulator of calmodulin and Ca2+/calmodulin-dependent kinase ...
Shao Hanshuang H Univ Pittsburgh, United - - 2014
Alpha-Actinin-4 (ACTN4), a key regulator of the actin cytoskeleton, is upregulated in melanoma, though its role in melanoma remains speculative. We have discovered that in WM1158, a highly aggressive melanoma cell line, downregulation of ACTN4 by shRNA induces a collagen I-dependent amoeboidal-to-mesenchymal transition. Re-expression of WT ACTN4 but not a ...
Christiaens Joaquin F JF Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), Department of Microbial and Molecular Systems, KU Leuven, Gaston Geenslaan 1, 3001 Leuven (Heverlee), Belgium; VIB Laboratory of Systems Biology, Gaston Geenslaan 1, 3001 Leuven (Heverlee), Belgium; Centre for Human Genetics, KU Leuven School of Medicine, Herestraat 49, 3000 Leuven, - - 2014
Yeast cells produce various volatile metabolites that are key contributors to the pleasing fruity and flowery aroma of fermented beverages. Several of these fruity metabolites, including isoamyl acetate and ethyl acetate, are produced by a dedicated enzyme, the alcohol acetyl transferase Atf1. However, despite much research, the physiological role of ...
Neumann Sarah S Microbiology, IWWR, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, the - - 2014
Anaerobic ammonium oxidizing (anammox) bacteria oxidize ammonium with nitrite to nitrogen gas in the absence of oxygen. These microorganisms form a significant sink for fixed nitrogen in the oceans and the anammox process is applied as a cost-effective and environment-friendly nitrogen removal system from wastewater. Anammox bacteria have a compartmentalized ...
Zaburdaev Vasily V School of Engineering and Applied Sciences, Harvard University, Cambridge, - - 2014
Neisseria gonorrheae bacteria are the causative agent of the second most common sexually transmitted infection in the world. The bacteria move on a surface by means of twitching motility. Their movement is mediated by multiple long and flexible filaments, called type IV pili, that extend from the cell body, attach ...
Tsikala Georgia G Institute of Molecular Biology and Biotechnology (IMBB) - Foundation for Research and Technology Hellas (FORTH), Nikolaou Plastira 100, GR-70013 Heraklion, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, P.O. Box 2208, GR-71003 Heraklion, Crete, - - 2014
The Drosophila respiratory system consists of two connected organs, the tracheae and the spiracles. Together they ensure the efficient delivery of air-borne oxygen to all tissues. The posterior spiracles consist internally of the spiracular chamber, an invaginated tube with filtering properties that connects the main tracheal branch to the environment, ...
Lockhart Marie M MM Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina, - - 2014
Recent studies using mouse models for cell fate tracing of epicardial derived cells (EPDCs) have demonstrated that at the atrioventricular (AV) junction EPDCs contribute to the mesenchyme of the AV sulcus, the annulus fibrosus, and the parietal leaflets of the AV valves. There is little insight, however, into the mechanisms ...
Xia Manhui M Department of Thoracic Surgery, Jingjiang People's Hospital of Jiangsu Province, Jingjiang City, Jiangsu Province, 214500, - - 2014
The mixed lineage leukemia 3 (MLL3), a member of the mixed lineage leukemia (MLL) family, has been reported to be mutated in multiple cancer types. However, its function in esophageal squamous cell carcinoma (ESCC) remains poorly understood. Here, we found that the expression of MLL3 was downregulated in ESCC tissues. ...
Dechanet-Merville Julie J CNRS UMR 5164, Universite Bordeaux - - 2014
γδ T cell response to cellular stress signals expressed by tumor cells make them promising candidates for cancer immunotherapy. The proof-of-concept for clinical scale propagation of polyclonal γδ T cell lines with efficient in vitro and in vivo response against cancer is an important step in this direction.
Wang Caixia - - 2014
Alteronol, isolated from a microbial mutation strains, have been applied for Chinese and international patents for tumor treatment. The aim of this project study is to investigate the characteristics of proliferation and redifferentiation induced by alteronol in melanoma B16F0 cells. Cell proliferation is determined by tetrazolium salt colorimetric method (MTT ...
Akter Jesmin J Laboratory of Innovative Cancer Therapeutics, Chiba Cancer Center Research Institute, Chiba 260-8717, - - 2014
We have previously identified neuronal leucine-rich repeat protein-3 (NLRR3) gene which was preferentially expressed in favorable human neuroblastomas as compared with unfavorable ones. In this study, we have found for the first time that NLRR3 is proteolytically processed by secretases and its intracellular domain (NLRR3-ICD) is then released to translocate ...
Motoyama Keiichi K Department of Physical Pharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, - - 2014
To design and evaluate the potential use of thioalkylated mannose-modified dendrimer (generation 3; G3) conjugates with α-cyclodextrin (Man-S-α-CDE (G3)) as novel antigen-presenting cell (APC)-selective siRNA carriers, we investigated the RNAi effects of siRNA complexes with Man-S-α-CDEs (G3). Man-S-α-CDE (G3, average degree of substitution of mannose (DSM) 4)/siRNA complex had the ...
Goliand I I Department of Life Sciences and the National Institute for Biotechnology in the Negev (NIBN), Ben-Gurion University, Beer-Sheva, 84105 - - 2014
Recently the ESCRT-III filamentous complex has been designated as the driving force for mammalian cell abscission, i.e. fission of the intercellular membrane bridge connecting daughter cells at the end of cytokinesis. However, how ESCRT-III is activated to set on abscission has not been resolved. Here we revisited the role of ...
Yamasaki Masao M Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan. myamasaki@cc.miyazaki-u.ac.jp - - 2014
Here, we examined the effect of tocotrienols (T3) on the growth of adult T-cell leukemia (ATL) cells. All three forms (β-, γ-, and δ-T3) inhibited cell proliferation in a dose-dependent manner; δ-T3 showed the strongest growth-inhibitory effect. δ-T3 increased the G1, G2/M, and subG1 populations and induced internucleosomal DNA fragmentation. ...
Liu Chunxi C Department of Stomatology, Xi'an No.4 Hospital (The Affiliated Guangren Hospital of Xi'an Jiaotong University College Medicine), Xi'an, 710004, China, - - 2014
microRNAs (miRNAs) are small non-coding RNAs that have been suggested to play an essential role in tumorigenesis. Reduced expression of miR-338 has been reported in several types of cancers; however, the role of miR-338 in oral squamous cell carcinoma (OSCC) has not been elucidated. In this study, we demonstrated that ...
Pattabhi Sudhakara Rao SR Department of Biological Science, Florida State University, Tallahassee, FL, 32306, - - 2014
Polyelectrolyte multilayer (PEMU) coatings built layer by layer with alternating pairs of polyelectrolytes can be tuned to improve cell interactions with surfaces and may be useful as biocompatible coatings to improve fixation between implants and tissues. Here, we show that human mesenchymal stromal cells (hMSCs) induced with bone differentiation medium ...
Chen Li-Chai LC Department of Pharmacy of Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, - - 2014
Two new thymol (=5-methyl-2-(1-methylethyl)phenol) derivatives, 8,10-didehydro-9-(3-methylbutanoyl)thymol 3-O-tiglate (1) and 9-O-angeloyl-8-methoxythymol 3-O-isobutyrate (2), were isolated from the root of Eupatorium cannabinum ssp. asiaticum, together with six known compounds, 3-8. The structures of 1 and 2 were determined through extensive 1D/2D-NMR and MS analyses. Among the isolates, 9-acetoxy-8,10-epoxythymol 3-O-tiglate (3) was the ...
Sega M M Department of Biotechnology, University of Verona, I-37134, Verona, - - 2014
Here, we propose a population ecology perspective to describe dynamic interplay between human leukaemia and cervical cancer cells growing together in the same environment. MOLT-3 (human T-lymphoblastic leukaemia) and HeLa (human cervical adenocarcinoma) cells were grown together or alone. Living cells were measured using flow cytometry, by counting propidium iodide-negative ...
Ponamoreva O N ON Department of Biotechnology, Tula State University, Pr. Lenina 92, Tula 300012, Russia. Electronic address: - - 2014
The methylotrophic Pichia angusta VKM Y-2559 and the oleaginous Cryptococcus curvatus VKM Y-3288 yeast cells were immobilized in a bimodal silica-organic sol-gel matrix comprised of tetraethoxysilane (TEOS), the hydrophobic additive methyltriethoxysilane (MTES) and the porogen polyethylene glycol (PEG). Under carefully optimized experimental conditions, employing basic catalysts, yeast cells have become ...
Yien Yvette Y - - 2014
The transport and intracellular trafficking of heme biosynthesis intermediates are crucial for hemoglobin production, which is a critical process in developing red cells. Here, we profiled gene expression in terminally differentiating murine fetal liver-derived erythroid cells to identify regulators of heme metabolism. We determined that TMEM14C, an inner mitochondrial membrane ...
Schoenfelder Kevin P KP Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, - - 2014
The endocycle is a modified cell cycle that lacks M phase. Endocycles are well known for enabling continued growth of post-mitotic tissues. By contrast, we discovered pre-mitotic endocycles in precursors of Drosophila rectal papillae (papillar cells). Unlike all known proliferative Drosophila adult precursors, papillar cells endocycle before dividing. Furthermore, unlike ...
Gueron Geraldine G IQUIBICEN-CONICET, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, CP C1428EGA, Buenos Aires, - - 2014
New d-ribofuranoside derivatives containing two five membered heterocycles, isoxazole and triazole or two triazole rings, were synthesized. The final products as well as the synthetic precursors were physically and spectroscopically characterized. These new diheterocyclic derivatives together with other d-riboside compounds were assessed for their impact on PC3 cell line viability. ...
Kenmoku Hiromichi - - 2014
To obtain the structural diversity of bioactive compounds similar to cotylenins and fusicoccins that modulate 14-3-3 protein-protein interactions in eukaryotes, screening tests were carried out using the lettuce seed dormancy breaking-assay. An acetone extract of the liverwort Plagiochila sciophila exhibited significant activity against the seeds in the presence of the ...
Li Yan Y Department of Obstetrics and Gynecology, Beijing Tongren Hospital, Capital Medical University , Beijing, - - 2014
The epithelial cell barrier function is a critical factor in the maintenance of the homeostasis of the vaginal mucosa. This study elucidates one of the mast cell-derived chemical mediators, tryptase, on compromising the vaginal epithelial barrier function. The results showed that human vaginal cell line, VK2, express PAR2. Activation of ...
Patel Samit A SA Department of Obstetrics and Gynecology, Madigan Army Medical Center, 9040 Jackson Avenue, Tacoma, WA - - 2014
A 54-year-old woman with a history of in-utero diethylstilbestrol (DES) exposure, who had a prior hysterectomy for symptomatic leiomyomata and dysmenorrhea, presented for vaginal bleeding. Vaginal biopsies showed a non-clear-cell adenocarcinoma, and the patient was subsequently treated with radiation therapy. We present a case of primary vaginal non-clear-cell adenocarcinoma in ...
Schultz Kevin J KJ Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, Calif., - - 2014
Background: The pathophysiology of pelvic organ prolapse (POP) involves vaginal collagen degradation. Strengthening collagen by UVA-photoactivated cross-linking has been demonstrated and suggested target applications include the vaginal wall. Aim: To identify UVA irradiation and riboflavin effects on vaginal cells. Materials and Methods: Vaginal cells were incubated for 24 h (DMEM/F-12 ...
Tayarani-Najaran Zahra Z Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail : - - 2014
Artemisia, as one of the largest genera in the tribe Anthemideae of the Asteraceae comprises an important part of Iranian flora. While cytotoxic and apoptotic properties have already been reported for some species of the genus there is not any report on cytotoxic effects of A. ciniformis. Petroleum ether (40-60), ...
Wu Shu-Zon SZ Department of Biology, University of Massachusetts, Amherst, Amherst, United - - 2014
Plant cells divide using the phragmoplast, a microtubule-based structure that directs vesicles secretion to the nascent cell plate. The phragmoplast forms at the cell center and expands to reach a specified site at the cell periphery, tens or hundreds of microns distant. The mechanism responsible for guiding the phragmoplast remains ...
Bitar Khalil N KN Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA. ; Department of Molecular Medicine and Translational Science, Wake Forest School of Medicine, Winston-Salem, NC, USA. ; Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Winston-Salem, NC, - - 2014
There are numerous available biodegradable materials that can be used as scaffolds in regenerative medicine. Currently, there is a huge emphasis on the designing phase of the scaffolds. Materials can be designed to have different properties in order to match the specific application. Modifying scaffolds enhances their bioactivity and improves ...
Rocha Débora Afonso Silva DA Universidade Santa Úrsula, Rio de Janeiro, - - 2013
Trichomonas vaginalis causes trichomoniasis in humans, a sexually transmitted disease commonly treated with metronidazole (MTZ). MTZ is known to cause undesirable side effects, and MTZ-resistant parasites have been reported. Thus, the development of an alternative treatment is desirable. Miltefosine (MLT) is an alkylphosphocholine synthetic lipid analogue that displays antiparasitic activity ...
Ghisalberti Carlo A - - 2013
To test the growth-promoting activity of the polyamine spermidine bound to various polymeric compounds in supramolecular complexes. A thiazolyl blue cell viability assay was used to determine the growth-promoting potency of spermidine-supramolecular complexes in a human skin fibroblast cell line exposed to spermidine and different spermidine-supramolecular complexes that were obtained ...
K Nivedita - - 2013
Spindle cell epithelioma is a very rare benign tumour of the vagina, which contains epithelial and mesenchymal components and co-expresses the markers for both. It has its origin in the epithelial cells of the remnants of the vestibular gland. The presence of glandular structures and the pattern of immunostaining, help ...
Laronda Monica M MM Division of Reproductive Biology Research, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, - - 2013
Women exposed to diethylstilbestrol (DES) in utero frequently develop vaginal adenosis, from which clear cell adenocarcinoma can arise. Despite decades of extensive investigation, the molecular pathogenesis of DES-associated vaginal adenosis remains elusive. Here we report that DES induces vaginal adenosis by inhibiting the BMP4/Activin A-regulated vaginal cell fate decision through ...
Sakai Kazuyoshi - - 2013
After finding tonsil-like structures near the entrance of vagina of a laboratory shrew (Suncus murinus), which we subsequently designated as vaginal tonsils, we performed detailed immunohistochemical and developmental studies. The location of T and B cells in the vaginal tonsils differed from that in the palatine tonsils or that in ...
Iacoponi Sara S Gynaecological Oncology Unit, La Paz University Hospital, Madrid, - - 2013
Adenocarcinoma of the cervix is a rare condition that has shown an increase in incidence, especially in the 20- to 34-year-old group. Adenocarcinoma represents about 5-10% of all tumours in this area, and, among these, the clear cell type accounts for 4-9%. This type of tumour affects mainly postmenopausal women ...
Chandra Neelima N CONRAD, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA 23507, - - 2013
The relationship between exogenous contraceptive hormones and permissiveness of the female genital tract to human immunodeficiency virus type 1 (HIV-1) is the subject of renewed debate. To better characterize the effect of depot medroxyprogesterone acetate (DMPA) on HIV-1 cellular targets and epithelial integrity in the vagina, we compared leukocyte populations, ...
Fowler R Stuart RS Mayo Clinic, Scottsdale, AZ 85259, USA. - - 2012
This study aimed to evaluate a new method for preparing vaginal wet preparations to enable quantification of cells and lactobacilli. The current nonstandardized technique allows for a variable amount of vaginal fluid collected, diluted by a variable amount of saline/KOH, and no quantification of constituents. The vaginal fluids from 100 ...
Bruin Jennifer E - - 2012
Fetal and neonatal nicotine exposure causes β-cell oxidative stress and apoptosis in neonates, leading to adult-onset dysglycemia. The aim of the present study was to determine whether an antioxidant intervention could prevent nicotine-induced β-cell loss. Nulliparous female Wistar rats received daily subcutaneous injections of either saline or nicotine bitartrate (1.0 ...
Schmid Frederike - - 2012
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a ubiquitous second messenger providing a Ca(2+) trigger in a wide range of cell types. However, its metabolism is not well understood. Here, we demonstrate the presence of endogenous NAADP in HeLa cells. CD38, a promiscuous enzyme described to be involved in NAADP ...
Da Silva Fernanda Rabaioli FR Department of Genetics and Molecular Biology, Postgraduate Program in Genetics and Molecular Biology (PPGBM), Federal University of Rio Grande do Sul (UFRGS), Porto Alegre-RS, - - 2012
Tobacco is a major Brazilian cash crop. Tobacco farmers apply large amounts of pesticides to control insect growth. Workers come into contact with green tobacco leaves during the tobacco harvest and absorb nicotine through the skin. In the present study, micronucleus frequency, cell death, and the frequency of basal cells ...
Al-Wadei Mohammed H - - 2012
Pancreatic ductal adenocarcinoma (PDAC) has a mortality rate near 100%. Smoking is a documented risk factor. However, the mechanisms of smoking-associated pancreatic carcinogenesis are poorly understood. We have shown that binding of nicotine to nicotinic acetylcholine receptors (nAChRs) expressing subunits α7, α3 and α5 in PDAC and pancreatic duct epithelial ...
Badisa Ramesh B - - 2012
Investigations with astroglial cells carry equal importance as those with neurons in drug abuse studies. The present study was aimed to investigate the effect of chronic cocaine administration on cell viability, nitric oxide (NO) production, general respiratory status of mitochondria and total protein levels in rat astroglioma cells after 24 ...
Tew Kenneth D KD Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425-5050, USA. - - 2012
The family of glutathione S-transferases (GSTs) is part of a cellular Phase II detoxification program composed of multiple isozymes with functional human polymorphisms that have the capacity to influence individual response to drugs and environmental stresses. Catalytic activity is expressed through GST dimer-mediated thioether conjugate formation with resultant detoxification of ...
Kao Liyo - - 2012
The patch clamp technique is widely used for recording the activity of ion channels in single cells and lipid bilayers. Most platforms utilize borosilicate glass configured as a pipette, however more recently planar patch clamp chips have been developed that require less technical expertise. Planar patch clamp chips in systems ...
Lee S-I - - 2012
Lee S-I, Kang K-L, Shin S-I, Herr Y, Lee Y-M, Kim E-C. Endoplasmic reticulum stress modulates nicotine-induced extracellular matrix degradation in human periodontal ligament cells. J Periodont Res 2012; doi: 10.1111/j.1600-0765.2012.01431.x. © 2012 John Wiley & Sons A/S Background and Objective:  Tobacco smoking is considered to be one of the ...
Bansal Himanta - - 2012
The assessment of micronuclei in exfoliated cells is a promising tool for the study of epithelial carcinogens and can be used to detect chromosome breakage or mitotic interference, thought to be relevant to carcinogenesis. The present study aimed to detect micronuclei in exfoliated oral mucosal cells in individuals using various ...
Ishizuka Toshiaki - - 2012
AIMS: As the clinical use of induced pluripotent stem (iPS) cells may have the potential to overcome current obstacles in stem cell-based therapy, the molecular mechanisms that regulate the proliferation of iPS cells are of great interest. However, to our knowledge, no previous studies have examined whether stimulation with nicotinic ...
Sakai Akiko - - 2012
Sr-containing carbonate apatite (SrCAp) specimens of varied Sr contents, ranging from 0 to 13.3 mol%, were prepared through a phosphate treatment of set gypsum-and-carbonate mixture at 100°C for 7 days. Effects of Sr content in SrCAp on microstructure, osteoblast-like cell (MC3T3-E1) attachment and proliferation, and alkaline phosphatase (ALP) activity were ...
Cucina Alessandra - - 2012
BACKGROUND: Colorectal cancer is one of the leading causes of cancer-related death throughout the world, and the risk to develop this malignant disease seems to be associated with long-term cigarette smoking. Nicotine, one of the major components of cigarette smoking, can stimulate cell proliferation and suppress apoptosis both in normal ...
Kalashnyk O M - - 2012
AIMS: Previously we demonstrated that mouse liver mitochondria express functional α7 nicotinic acetylcholine receptors (nAChRs). The aim of this study was to investigate whether the nAChRs are found in mitochondria of non-neuronal human cell lines. MAIN METHODS: Three cell lines: U373 (astrocytes), U937 (monocytes) and Daudi (B lymphocytes) were examined ...
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