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Sugimoto K K Department of Cariology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, - - 2014
iPS cells are attractive for the regenerative medicine. The creation of pluripotent cells from somatic cells has great potential for basic and clinical research and application. Retroviral transduction of four or three transfection factors has been shown to initiate a reprogramming process. Here, we describe the effect of transcription factors ...
Matusek Tamás T 1] Université de Nice Sophia Antipolis, iBV, UMR 7277, 06100 Nice, France [2] CNRS, iBV, UMR 7277, 06100 Nice, France [3] INSERM, iBV, U1091, 06100 Nice, - - 2014
The conserved family of Hedgehog (Hh) proteins acts as short- and long-range secreted morphogens, controlling tissue patterning and differentiation during embryonic development. Mature Hh carries hydrophobic palmitic acid and cholesterol modifications essential for its extracellular spreading. Various extracellular transportation mechanisms for Hh have been suggested, but the pathways actually used ...
Collins Scott Forrest SF TeVido BioDevices, LLC , Austin, - - 2014
Abstract 3D printing, or solid freeform fabrication, applied to regenerative medicine brings technologies from several industries together to help solve unique challenges in both basic science and tissue engineering. By more finely organizing cells and supporting structures precisely in 3D space, we will gain critical knowledge of cell-cell communications and ...
Hsieh Meng-Hsun MH Department of Microbiology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei, 100, Taiwan, - - 2014
Telomere maintenance is required for chromosome stability, and telomeres are typically elongated by telomerase following DNA replication. In both tumor and yeast cells that lack telomerase, telomeres are maintained via an alternative recombination mechanism. Previous studies have indicated that yeast Sgs1 and Top3 may work together to remove highly negative ...
Haubrich Brad A BA Texas Tech University, United - - 2014
Ergosterol biosynthesis and homeostasis in the parasitic protozoan Trypanosoma brucei was analyzed by RNAi silencing and inhibition of sterol C24β-methyltransferase (TbSMT) and sterol 14α-demethylase (TbSDM = TbCYP51) to explore the functions of sterols in T. brucei growth. Inhibition of the amount or activity of these enzymes depletes ergosterol from cells ...
Wang Teng T the Affiliated Hospital of Jiangnan University and the Fourth People's Hospital of Wuxi, - - 2014
5-Fluorouracil (5-Fu) is commonly used in the chemotherapy of colorectal cancer (CRC), but resistance to 5-Fu occurs in most cases, allowing cancer progression. Suppressing ABCB1 (ATP-binding cassette, sub-family B, member1), which is a pump overproduced in cancer cells to export cytotoxic drugs, is an attractive strategy to overcome drug resistance. ...
Chen Haining H Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. - - 2014
Hybrid organic/inorganic perovskite solar cells are attracting intense attention and further developments largely hinge on understanding the fundamental issues involved in the cell operation. In this paper, a liquid phase deposition (LPD) method is developed to design and grow a TiO2 nanolayer at room temperature for carbon-based perovskite solar cells. ...
Zhang Dexiang D General Surgery Department, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai, 200032, - - 2014
Hepatocellular carcinoma (HCC) is one of the most common malignancies with a poor response to chemotherapy. It is very important to identify novel diagnosis biomarkers and therapeutic targets. RIP140, a regulator of estrogen receptor, recently has been found to be involved in the tumorigenesis. However, its function in the progression ...
Meng Ke - - 2014
For the first time, a quasi-solid thiolate/disulfide based electrolyte was prepared using succinonitrile as a matrix. An optimized configuration of the quasi-solid electrolyte contains TMAT/T2/LiClO4/NMBII in the ratio of 0.8:0.8:0.1:0.1M. Dye sensitized solar cells fabricated using this quasi-solid electrolyte, together with N719 dye sensitized photoelectrode and CoS counter electrode attained ...
Guillaume Olivier O Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Ireland Department of Mechanical Engineering, School of Engineering, Trinity College Dublin, - - 2014
Lower lumbar disc disorders pose a significant problem in an aging society with substantial socioeconomic consequences. Both inner tissue (nucleus pulposus) and outer tissue (annulus fibrosus) of the intervertebral disc are affected by such debilitating disorders and can lead to disc herniation and lower back pain. In this study, we ...
Mahale Sachin - - 2014
Biphenyl-4-carboxylic acid-[2-(1H-indol-3-yl)-ethyl]-methylamide 1 (CA224) is a non-planar analog of fascaplysin (2) that specifically inhibits Cdk4-cyclin D1 in-vitro. Compound 1 blocks growth of cancer cells at G0/G1 phase of the cell cycle. It also blocks at G2/M phase which is explained by the fact that it inhibits tubulin polymerization. Besides, it ...
Quintana Jairo J Departamento de Química, Universidad Nacional de Colombia, Carrera 30 N° 45-03, Bogotá, - - 2014
Marine benthic cyanobacteria are widely known as a source of toxic and potentially useful compounds. These microorganisms have been studied from many Caribbean locations, which recently include locations in the Colombian Caribbean Sea. In the present study, six lipopeptides named almiramides D to H, together with the known almiramide B ...
Jiang Wei W Department of Thoracic Surgery, Taixing People's Hospital of Jiangsu Province, 1 of Changzheng Rd, Taixing City, Jiangsu Province, 225400, - - 2014
VGLL4, a member of the Vestigial-like (VGLL) proteins, has been reported to be dysregulated in several cancer types. However, its function in esophageal squamous cell carcinoma (ESCC) remains poorly understood. Here, it was found that the expression level of VGLL4 was decreased in ESCC tissues. Moreover, forced expression of VGLL4 ...
Hasegawa Kazuteru - - 2014
In mammalian testes, spermatogonial stem cells (SSCs) maintain spermatogenesis over a long period of time by undergoing self-renewal and differentiation. SSCs are among the most primitive of spermatogenic cells (undifferentiated spermatogonia) and their activities are strictly regulated by extrinsic niche factors. However, the factors that constitute a testicular niche remain ...
Schaffrath Raffael R Department of Genetics, University of Leicester, Leicester LE1 7RH, UK; Institut für Biologie, Abteilung Mikrobiologie, Universität Kassel, 34132 Kassel, - - 2014
Diphthamide is a conserved modification in archaeal and eukaryal translation elongation factor 2 (EF2). Its name refers to the target function for diphtheria toxin, the disease-causing agent which through ADP ribosylation of diphthamide causes irreversible inactivation of EF2 and cell death. Although this clearly emphasizes a pathobiological role for diphthamide, ...
Peng Hongwei H Laboratory of Cancer Biotherapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. - - 2014
Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children. The differentiation status of neuroblastoma is correlated with clinical outcome, and the induction of differentiation thus constitutes a therapeutic approach in this disease. However, the molecular mechanisms that control ...
Manukyan Arkadi - - 2014
The cytokinetic furrow (CF) is organized by the RhoA GTPase, which recruits actin and myosin II to the furrow and drives contractility. Here we show a role for the RhoGAP, p190, in cytokinesis and its involvement in regulating Rho GTP levels and contractility. Cells depleted of p190RhoGAP (p190) accumulate high ...
Devenport Danelle D Department of Molecular Biology, Princeton University, Princeton, NJ 08544 - - 2014
Planar cell polarity (PCP) refers to the coordinated alignment of cell polarity across the tissue plane. Key to the establishment of PCP is asymmetric partitioning of cortical PCP components and intercellular communication to coordinate polarity between neighboring cells. Recent progress has been made toward understanding how protein transport, endocytosis, and ...
Jeong Moon Hee MH College of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Republic of - - 2014
Mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease caused by mutations in the mitochondrial genome. This study investigated the efficacy of β-lapachone (β-lap), a natural quinone compound, in rescuing mitochondrial dysfunction in MELAS cybrid cells. β-Lap significantly restored energy production and mitochondrial membrane potential as ...
Oliveira Helena H CESAM & Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address: - - 2014
Cadmium (Cd) is a widespread heavy metal used in numerous industrial processes. Cd exerts toxicological effects mostly in kidney and liver. Bone is also an important target of Cd, however, the cellular mechanisms of Cd toxicological effects in the bone cells are still poorly understood. Therefore, the present work aimed ...
Jennings Paul P Division of Physiology, Dept. of Physiology and Medical Physics, Innsbruck Medical University, Fritz-Pregl Strasse 3/1, Innsbruck 6020, Austria. Electronic address: - - 2014
In 1959 Russell and Burch stated that "Mammalian tissue cultures have become one of the most important replacement techniques". Although they would likely be disappointed at the slow progress towards the 3Rs, I am sure they would be amazed and excited about the advances made. For example, omic technologies now ...
Mazar Joseph J Sanford-Burnham Medical Research Institute, Orlando, FL 32827, - - 2014
Expression of the long noncoding RNA (lncRNA) SPRY4-IT1 is low in normal human melanocytes but high in melanoma cells. siRNA knockdown of SPRY4-IT1 blocks melanoma cell invasion and proliferation, and increases apoptosis. To investigate its function further, we affinity purified SPRY4-IT1 from melanoma cells and used mass spectrometry to identify ...
Shao Hanshuang H Univ Pittsburgh, United - - 2014
Alpha-Actinin-4 (ACTN4), a key regulator of the actin cytoskeleton, is upregulated in melanoma, though its role in melanoma remains speculative. We have discovered that in WM1158, a highly aggressive melanoma cell line, downregulation of ACTN4 by shRNA induces a collagen I-dependent amoeboidal-to-mesenchymal transition. Re-expression of WT ACTN4 but not a ...
Christiaens Joaquin F JF Laboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), Department of Microbial and Molecular Systems, KU Leuven, Gaston Geenslaan 1, 3001 Leuven (Heverlee), Belgium; VIB Laboratory of Systems Biology, Gaston Geenslaan 1, 3001 Leuven (Heverlee), Belgium; Centre for Human Genetics, KU Leuven School of Medicine, Herestraat 49, 3000 Leuven, - - 2014
Yeast cells produce various volatile metabolites that are key contributors to the pleasing fruity and flowery aroma of fermented beverages. Several of these fruity metabolites, including isoamyl acetate and ethyl acetate, are produced by a dedicated enzyme, the alcohol acetyl transferase Atf1. However, despite much research, the physiological role of ...
Kim Eugene E - - 2014
Cardiac Purkinje cells are important triggers of ventricular arrhythmias associated with heritable and acquired syndromes; however, the mechanisms responsible for this proarrhythmic behavior are incompletely understood. Here, through transcriptional profiling of genetically labeled cardiomyocytes, we identified expression of Purkinje cell protein-4 (Pcp4), a putative regulator of calmodulin and Ca2+/calmodulin-dependent kinase ...
Neumann Sarah S Microbiology, IWWR, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, the - - 2014
Anaerobic ammonium oxidizing (anammox) bacteria oxidize ammonium with nitrite to nitrogen gas in the absence of oxygen. These microorganisms form a significant sink for fixed nitrogen in the oceans and the anammox process is applied as a cost-effective and environment-friendly nitrogen removal system from wastewater. Anammox bacteria have a compartmentalized ...
Zaburdaev Vasily V School of Engineering and Applied Sciences, Harvard University, Cambridge, - - 2014
Neisseria gonorrheae bacteria are the causative agent of the second most common sexually transmitted infection in the world. The bacteria move on a surface by means of twitching motility. Their movement is mediated by multiple long and flexible filaments, called type IV pili, that extend from the cell body, attach ...
Tsikala Georgia G Institute of Molecular Biology and Biotechnology (IMBB) - Foundation for Research and Technology Hellas (FORTH), Nikolaou Plastira 100, GR-70013 Heraklion, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, P.O. Box 2208, GR-71003 Heraklion, Crete, - - 2014
The Drosophila respiratory system consists of two connected organs, the tracheae and the spiracles. Together they ensure the efficient delivery of air-borne oxygen to all tissues. The posterior spiracles consist internally of the spiracular chamber, an invaginated tube with filtering properties that connects the main tracheal branch to the environment, ...
Lockhart Marie M MM Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, - - 2014
Recent studies using mouse models for cell fate tracing of epicardial derived cells (EPDCs) have demonstrated that at the atrioventricular (AV) junction EPDCs contribute to the mesenchyme of the AV sulcus, the annulus fibrosus, and the parietal leaflets of the AV valves. There is little insight, however, into the mechanisms ...
Xia Manhui M Department of Thoracic Surgery, Jingjiang People's Hospital of Jiangsu Province, Jingjiang City, Jiangsu Province, 214500, - - 2014
The mixed lineage leukemia 3 (MLL3), a member of the mixed lineage leukemia (MLL) family, has been reported to be mutated in multiple cancer types. However, its function in esophageal squamous cell carcinoma (ESCC) remains poorly understood. Here, we found that the expression of MLL3 was downregulated in ESCC tissues. ...
Dechanet-Merville Julie J CNRS UMR 5164, Universite Bordeaux - - 2014
γδ T cell response to cellular stress signals expressed by tumor cells make them promising candidates for cancer immunotherapy. The proof-of-concept for clinical scale propagation of polyclonal γδ T cell lines with efficient in vitro and in vivo response against cancer is an important step in this direction.
Wang Caixia - - 2014
Alteronol, isolated from a microbial mutation strains, have been applied for Chinese and international patents for tumor treatment. The aim of this project study is to investigate the characteristics of proliferation and redifferentiation induced by alteronol in melanoma B16F0 cells. Cell proliferation is determined by tetrazolium salt colorimetric method (MTT ...
Akter Jesmin J Laboratory of Innovative Cancer Therapeutics, Chiba Cancer Center Research Institute, Chiba 260-8717, - - 2014
We have previously identified neuronal leucine-rich repeat protein-3 (NLRR3) gene which was preferentially expressed in favorable human neuroblastomas as compared with unfavorable ones. In this study, we have found for the first time that NLRR3 is proteolytically processed by secretases and its intracellular domain (NLRR3-ICD) is then released to translocate ...
Motoyama Keiichi K Department of Physical Pharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, - - 2014
To design and evaluate the potential use of thioalkylated mannose-modified dendrimer (generation 3; G3) conjugates with α-cyclodextrin (Man-S-α-CDE (G3)) as novel antigen-presenting cell (APC)-selective siRNA carriers, we investigated the RNAi effects of siRNA complexes with Man-S-α-CDEs (G3). Man-S-α-CDE (G3, average degree of substitution of mannose (DSM) 4)/siRNA complex had the ...
Goliand Inna I Department of Life Sciences and the National Institute for Biotechnology in the Negev (NIBN), Ben-Gurion University of the Negev, Beer-Sheva 84105, - - 2014
Recently the ESCRT-III filamentous complex was designated as the driving force for mammalian cell abscission, that is, fission of the intercellular membrane bridge connecting daughter cells at the end of cytokinesis. However, how ESCRT-III is activated to set on abscission has not been resolved. Here we revisit the role of ...
Yamasaki Masao M Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan. myamasaki@cc.miyazaki-u.ac.jp - - 2014
Here, we examined the effect of tocotrienols (T3) on the growth of adult T-cell leukemia (ATL) cells. All three forms (β-, γ-, and δ-T3) inhibited cell proliferation in a dose-dependent manner; δ-T3 showed the strongest growth-inhibitory effect. δ-T3 increased the G1, G2/M, and subG1 populations and induced internucleosomal DNA fragmentation. ...
Liu Chunxi C Department of Stomatology, Xi'an No.4 Hospital (The Affiliated Guangren Hospital of Xi'an Jiaotong University College Medicine), Xi'an, 710004, China, - - 2014
microRNAs (miRNAs) are small non-coding RNAs that have been suggested to play an essential role in tumorigenesis. Reduced expression of miR-338 has been reported in several types of cancers; however, the role of miR-338 in oral squamous cell carcinoma (OSCC) has not been elucidated. In this study, we demonstrated that ...
Pattabhi Sudhakara Rao SR Department of Biological Science, Florida State University, Tallahassee, FL, 32306, - - 2014
Polyelectrolyte multilayer (PEMU) coatings built layer by layer with alternating pairs of polyelectrolytes can be tuned to improve cell interactions with surfaces and may be useful as biocompatible coatings to improve fixation between implants and tissues. Here, we show that human mesenchymal stromal cells (hMSCs) induced with bone differentiation medium ...
Chen Li-Chai LC Department of Pharmacy of Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, - - 2014
Two new thymol (=5-methyl-2-(1-methylethyl)phenol) derivatives, 8,10-didehydro-9-(3-methylbutanoyl)thymol 3-O-tiglate (1) and 9-O-angeloyl-8-methoxythymol 3-O-isobutyrate (2), were isolated from the root of Eupatorium cannabinum ssp. asiaticum, together with six known compounds, 3-8. The structures of 1 and 2 were determined through extensive 1D/2D-NMR and MS analyses. Among the isolates, 9-acetoxy-8,10-epoxythymol 3-O-tiglate (3) was the ...
Sega M M Department of Biotechnology, University of Verona, I-37134, Verona, - - 2014
Here, we propose a population ecology perspective to describe dynamic interplay between human leukaemia and cervical cancer cells growing together in the same environment. MOLT-3 (human T-lymphoblastic leukaemia) and HeLa (human cervical adenocarcinoma) cells were grown together or alone. Living cells were measured using flow cytometry, by counting propidium iodide-negative ...
Ponamoreva O N ON Department of Biotechnology, Tula State University, Pr. Lenina 92, Tula 300012, Russia. Electronic address: - - 2014
The methylotrophic Pichia angusta VKM Y-2559 and the oleaginous Cryptococcus curvatus VKM Y-3288 yeast cells were immobilized in a bimodal silica-organic sol-gel matrix comprised of tetraethoxysilane (TEOS), the hydrophobic additive methyltriethoxysilane (MTES) and the porogen polyethylene glycol (PEG). Under carefully optimized experimental conditions, employing basic catalysts, yeast cells have become ...
Yien Yvette Y - - 2014
The transport and intracellular trafficking of heme biosynthesis intermediates are crucial for hemoglobin production, which is a critical process in developing red cells. Here, we profiled gene expression in terminally differentiating murine fetal liver-derived erythroid cells to identify regulators of heme metabolism. We determined that TMEM14C, an inner mitochondrial membrane ...
Schoenfelder Kevin P KP Department of Pharmacology and Cancer Biology, Box 3813, Duke University Medical Center, Durham, NC 27710, - - 2014
The endocycle is a modified cell cycle that lacks M phase. Endocycles are well known for enabling continued growth of post-mitotic tissues. By contrast, we discovered pre-mitotic endocycles in precursors of Drosophila rectal papillae (papillar cells). Unlike all known proliferative Drosophila adult precursors, papillar cells endocycle before dividing. Furthermore, unlike ...
Gueron Geraldine G IQUIBICEN-CONICET, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, CP C1428EGA, Buenos Aires, - - 2014
New d-ribofuranoside derivatives containing two five membered heterocycles, isoxazole and triazole or two triazole rings, were synthesized. The final products as well as the synthetic precursors were physically and spectroscopically characterized. These new diheterocyclic derivatives together with other d-riboside compounds were assessed for their impact on PC3 cell line viability. ...
Kenmoku Hiromichi - - 2014
To obtain the structural diversity of bioactive compounds similar to cotylenins and fusicoccins that modulate 14-3-3 protein-protein interactions in eukaryotes, screening tests were carried out using the lettuce seed dormancy breaking-assay. An acetone extract of the liverwort Plagiochila sciophila exhibited significant activity against the seeds in the presence of the ...
Li Yan Y Department of Obstetrics and Gynecology, Beijing Tongren Hospital, Capital Medical University , Beijing, - - 2014
The epithelial cell barrier function is a critical factor in the maintenance of the homeostasis of the vaginal mucosa. This study elucidates one of the mast cell-derived chemical mediators, tryptase, on compromising the vaginal epithelial barrier function. The results showed that human vaginal cell line, VK2, express PAR2. Activation of ...
Patel Samit A SA Department of Obstetrics and Gynecology, Madigan Army Medical Center, 9040 Jackson Avenue, Tacoma, WA - - 2014
A 54-year-old woman with a history of in-utero diethylstilbestrol (DES) exposure, who had a prior hysterectomy for symptomatic leiomyomata and dysmenorrhea, presented for vaginal bleeding. Vaginal biopsies showed a non-clear-cell adenocarcinoma, and the patient was subsequently treated with radiation therapy. We present a case of primary vaginal non-clear-cell adenocarcinoma in ...
Schultz Kevin J KJ Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, Calif., - - 2014
Background: The pathophysiology of pelvic organ prolapse (POP) involves vaginal collagen degradation. Strengthening collagen by UVA-photoactivated cross-linking has been demonstrated and suggested target applications include the vaginal wall. Aim: To identify UVA irradiation and riboflavin effects on vaginal cells. Materials and Methods: Vaginal cells were incubated for 24 h (DMEM/F-12 ...
Tayarani-Najaran Zahra Z Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail : - - 2014
Artemisia, as one of the largest genera in the tribe Anthemideae of the Asteraceae comprises an important part of Iranian flora. While cytotoxic and apoptotic properties have already been reported for some species of the genus there is not any report on cytotoxic effects of A. ciniformis. Petroleum ether (40-60), ...
Bitar Khalil N KN Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA. ; Department of Molecular Medicine and Translational Science, Wake Forest School of Medicine, Winston-Salem, NC, USA. ; Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Winston-Salem, NC, - - 2014
There are numerous available biodegradable materials that can be used as scaffolds in regenerative medicine. Currently, there is a huge emphasis on the designing phase of the scaffolds. Materials can be designed to have different properties in order to match the specific application. Modifying scaffolds enhances their bioactivity and improves ...
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