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Basu Joydeep J Process Research & Translation, Tengion, Inc., 3929 Westpoint Blvd, Suite G, Winston-Salem, NC 27103, - - 2014
Potency is a critical quality attribute of biological products, defined by the US FDA as the specific ability or capacity of the product, as indicated by appropriate laboratory tests or by adequately controlled clinical data obtained through the administration of the product in the manner intended, to effect a given ...
Nakayama T T Global Collaboration Center, Osaka University, 1-16 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan, - - 2014
Streptococcus species release cholesterol-dependent cytolysins (CDCs), which are a main toxin, and their heat susceptibility is poorly understood. The aim of this study was to clarify the heat susceptibility of streptococcal exotoxins and CDCs. Streptococcal exotoxins were treated with heat incubation at 60 °C for 10 or 30 min. The Streptococcus suis ...
Mailey Brian B Department of Surgery, University of California San Diego, San Diego, CA, USA, - - 2014
Adipose tissue sciences have rapidly expanded since the identification of regenerative cells contained within the stromal vascular fraction (SVF) of fat. Isolation of the SVF, containing adipose-derived stem cells (ADSC), can be accomplished efficiently in the operating room or in the laboratory through enzymatic digestion of the adipose tissue and ...
Lan H - - 2012
The G protein-coupled receptor 119 (GPR119) mediates insulin secretion from pancreatic β cells and glucagon-like peptide 1 (GLP-1) release from intestinal L cells. While GPR119-mediated insulin secretion is glucose dependent, it is not clear whether or not GPR119-mediated GLP-1 secretion similarly requires glucose. This study was designed to address the ...
Zhu Xinping - - 2012
This study aimed to explore the effect of high glucose concentration on the expression of glial cell line-derived neurotrophic factor (GDNF) and its family ligand receptors (GFRs) GFRα1 and GFRα2 in Müller cells and the protective role of GDNF in cultured Müller cells under high glucose circumstance. Cultured Müller cells ...
Hoose Scott A - - 2012
Screening chemical libraries to identify compounds that affect overall cell proliferation is common. However, in most cases, it is not known whether the compounds tested alter the timing of particular cell cycle transitions. Here, we evaluated an FDA-approved drug library to identify pharmaceuticals that alter cell cycle progression in yeast, ...
Wu Hao - - 2011
Solid tumors are dependent on glucose, but are generally glucose-deprived due to poor vascularization. Nevertheless cancer cells can generally survive glucose deprivation better than their normal counterparts. Thus, to render cancer cells sensitive to glucose depletion may potentially provide an effective strategy for cancer intervention. We propose that lactic acidosis, ...
Zhang Hongli - - 2011
Menin, encoded by the Men1 gene, is responsible for β-cell tumor formation in patients with multiple endocrine neoplasia type 1. Recently, menin has been proven to negatively regulate β-cell proliferation during pregnancy. However, it is unclear whether menin is involved in pancreatic β-cell proliferation in response to other physiological replication ...
Roma Leticia P - - 2011
Using the reactive oxygen species-sensitive fluorescent dyes dichlorodihydrofluorescein and dihydroethidine, previous studies yielded opposite results about the glucose regulation of oxidative stress in insulin-secreting pancreatic β-cells. Here, we used the ratiometric fluorescent proteins HyPer and roGFP1 targeted to mitochondria (mt-HyPer/mt-roGFP1) to monitor glucose-induced changes in mitochondrial hydrogen peroxide concentration (mt-HyPer) ...
Cao Dongwei - - 2011
Background: Long-term peritoneal exposure to high glucose in the peritoneal dialysis (PD) solution may potentiate the development of peritoneal fibrosis in PD patients. The most important factor leading to peritoneal fibrosis may be injury of human peritoneal mesothelial cells (HPMC). Little is known about senescence of HPMC. It has been ...
Zheng Ye Y Department of Surgery and the Gastroenterology Research Unit, Mayo Clinic, Rochester, MN 55905, - - 2012
GLUT2 is translocated to the apical membrane of enterocytes exposed to glucose concentrations >∼50 mM. Mechanisms of GLUT2-mediated glucose uptake in cell culture models of enterocytes have not been studied. To explore mechanism(s) of glucose uptake in 3 enterocyte-like cell lines. Glucose uptake was measured in Caco-2, RIE-1, and IEC-6 ...
Liu Zhen - - 2011
Production of highly pure (2S,3S)-2,3-butanediol ((2S,3S)-2,3-BD) and (3S)-acetoin ((3S)-AC) in high concentrations is desirable but difficult to achieve. In the present study, glucose was first transformed to a mixture of (2S,3S)-2,3-BD and meso-2,3-BD by resting cells of Klebsiella pneumoniae CICC 10011, followed by biocatalytic resolution of the mixture by resting ...
Jackson Alicia R AR Tissue Biomechanics Lab, Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, - - 2011
The intervertebral disc (IVD) receives important nutrients, such as glucose, from surrounding blood vessels. Poor nutritional supply is believed to play a key role in disc degeneration. Several investigators have presented finite element models of the IVD to investigate disc nutrition; however, none has predicted nutrient levels and cell viability ...
Andriunas Felicity A - - 2011
Transfer cells (TCs) are specialized transport cells containing invaginated wall ingrowths that generate an amplified plasma membrane surface area with high densities of transporter proteins. They trans-differentiate from differentiated cells at sites where enhanced rates of nutrient transport occur across apo-/symplasmic boundaries. Despite their physiological importance, the signal(s) and signalling ...
Oliveira P F - - 2011
Sertoli cells metabolize glucose, converting it to lactate that is used by developing germ cells for their energy metabolism. Androgens and oestrogens have metabolic roles that reach far beyond reproductive processes. So, the main purpose of this study was to examine the effect of sex steroid hormones on metabolite secretion/consumption ...
Dubuisson Olga - - 2011
Although thiazolidinediones (TZD) effectively improve hyperglycemia and increase adiponectin, a proinsulin-sensitizing adipokine, they also increase adipogenesis via peroxisome proliferator-activated receptor (PPAR)γ induction, which may be undesirable. Recent safety concerns about some TZD have prompted the search for next generation agents that can enhance glycemic control and adiponectin independent of PPARγ ...
Sonko Bakary J - - 2011
The lichen metabolite usnic acid (UA) has been promoted as a dietary supplement for weight loss, although cases of hepatotoxicity have been reported. Here we evaluated usnic acid-associated hepatotoxicity in vitro using isolated rat hepatocytes. We measured cell viability and ATP content to evaluate UA induced cytotoxicity and applied (13)C ...
Clark A R - - 2011
In vitro maturation (IVM) of mammalian oocytes provides an alternative to traditional in vitro fertilization techniques for clinical treatment of infertility or animal breeding. IVM involves the collection of oocytes from the ovary prior to ovulation, with maturation occurring in a laboratory environment. The success of IVM is highly sensitive ...
Frolova Antonina I AI Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110, - - 2011
Endometrial stromal cells (ESC) must undergo a hormone-driven differentiation to form decidual cells as a requirement of proper embryo implantation. Recent studies from our laboratory have demonstrated that decidualizing cells require glucose transporter 1 expression and an increase in glucose use to complete this step. The present study focuses on ...
Coloff Jonathan L JL Departments of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, - - 2011
Most cancer cells utilize aerobic glycolysis, and activation of the phosphoinositide 3-kinase/Akt/mTOR pathway can promote this metabolic program to render cells glucose dependent. Although manipulation of glucose metabolism may provide a means to specifically eliminate cancer cells, mechanistic links between cell metabolism and apoptosis remain poorly understood. Here, we examined ...
Zhang Lan-Yu - - 2011
To investigate the effects of beraprost sodium on the proliferation and oxidative stress of glomerular mesangial cells under high glucose conditions, a rat mesangial cell line (rat mesangial cells; RMCs) was treated with beraprost sodium in the presence of high glucose concentrations. Proliferation rates of mesangial cells were detected by ...
Natoli Manuela - - 2011
The human intestinal Caco-2 cell line has been extensively used as a model of the intestinal barrier. However, it is widely reported in literature that culture-related conditions, as well as the different Caco-2 cell lines utilized in different laboratories, often lead to problems of reproducibility making difficult to compare results. ...
Knaupp Markus - - 2011
A role of non-reducing sugars like sucrose and raffinose in the protection of plant cells against damage during freezing has been proposed for many species, but reports on physiological effects are conflicting. Non-aqueous fractionation of mesophyll cell compartments in Arabidopsis thaliana was used to show that sucrose and raffinose accumulate ...
Plocinski P P The University of Texas Health Science Center, Tyler, TX 75708, - - 2011
The role(s) in cell division of the Mycobacterium tuberculosis Rv0011c gene product, a homolog of the Streptomyces CrgA protein that is responsible for coordinating growth and cytokinesis in sporogenic aerial hyphae, is largely unknown. We show that an enhanced cyan fluorescent protein-M. tuberculosis CrgA (ECFP-CrgA(MT)) fusion protein is localized to ...
Perfito Nicole - - 2011
The ability to breed at any time of year enables opportunistically breeding species to respond to good conditions whenever they occur. We investigate the neuroendocrine basis for this relatively unusual reproductive pattern in the avian world. One proposed mechanism for year-round breeding ability is tonic activation of gonadotropin-releasing hormone-I (GnRH-I) ...
Ye Yan - - 2011
AIM OF THE STUDY: Atractylenolide II (AT-II) is a sesquiterpene compound isolated from the dried rhizome of Atractylodes macrocephala (Baizhu in Chinese), which is traditionally prescribed for melanoma treatment by Chinese medicine practitioners. Our previous study showed that AT-II can inhibit B16 cells proliferation. Here we investigated the mechanistic basis ...
Kudela Pavol - - 2011
The purpose of the presented investigation was to examine the efficiency of the novel carrier system Bacterial Ghosts (BGs), which are empty bacterial cell envelopes of Gram-negative bacteria to target human conjunctival epithelial cells, as well as to test the endocytic capacity of conjunctival cells after co-incubation with BGs generated ...
Matsumura Fumio - - 2011
At mitosis, cells undergo drastic alterations in morphology and cytoskeletal organization including cell rounding during prophase, mitotic spindle assembly during prometaphase and metaphase, chromatid segregation in anaphase, and cytokinesis during telophase. It is well established that myosin II is a motor responsible for cytokinesis. Recent reports have indicated that myosin ...
Bai Yu - - 2011
Based upon the bis(2,9-dimethyl-1,10-phenanthroline)copper(i/ii) electron mediator, we present an iodine-free dye-sensitized solar cell exhibiting an impressive power conversion efficiency of 7.0% at 100 mW cm(-2) air mass global (AM1.5G) conditions, which rivals that of a control cell with a conventional iodine redox couple.
Suaid Fabricia Ferreira - - 2011
Suaid FF, Ribeiro FV, Rodrigues TL, Silvério KG, Carvalho MD, Nociti Jr. FH, Casati MZ, Sallum EA: Autologous periodontal ligament cells in the treatment of class II furcation defects: a study in dogs. J Clin Periodontol 2011; doi: 10.1111/j.1600-051X.2010.01715.x ABSTRACT: Aim: The goal of this study was to histologically investigate ...
Valente Sergio - - 2011
Four novel series of cinnamyl-containing histone deacetylase (HDAC) inhibitors 1-4 are described, containing hydroxamate (1 and 3) or 2-aminoanilide (2 and 4) derivatives. When screened against class I (maize HD1-B and human HDAC1) and class II (maize HD1-A and human HDAC4) HDACs, most hydroxamates and 2-aminoanilides displayed potent and selective inhibition toward ...
Yang Hailing H Department of Integrative Biology and Pharmacology, University of Texas Medical School, Houston, Texas 77030, - - 2011
Class VI β-tubulin (β6) is the most divergent tubulin produced in mammals and is found only in platelets and mature megakaryocytes. To determine how this unique tubulin isotype affects microtubule assembly and organization, we expressed the cDNA in tissue culture cells under the control of a tetracycline regulated promoter. The ...
Fazlollahi Farnoosh F Will Rogers Institute Pulmonary Research Center, University of Southern California, Los Angeles, California 90033, USA. - - 2011
Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN(3)) or decreased temperature), and endocytosis ...
Eekhoff Alexander - - 2011
Kidney glomeruli function as filters, allowing the passage of small solutes and waste products into the urinary tract, while retaining essential proteins and macromolecules in the blood stream. These structures are under constant mechanical stress due to fluid pressure, driving filtration across the barrier. We mechanically stimulated adherent wildtype podocytes ...
Shahidi-Noghabi Shahnaz - - 2011
In this project, the uptake mechanisms and localization of two lectins from Sambucus nigra, further referred to as S. nigra agglutinin (SNA)-I and SNA-II, into insect midgut CF-203 cells were studied. SNA-I is a chimeric lectin belonging to the class of ribosome-inactivating proteins, whereas SNA-II is a hololectin devoid of ...
Kondo Tomo - - 2011
Myosin II ATPase activity is enhanced by the phosphorylation of its regulatory light chain (MRLC) in non-muscle cells. It is well known that phosphorylated MRLC (P-MRLC) colocalizes with F-actin in the contractile ring (CR) of dividing cells. Recently, we reported that HeLa cells expressing non-phosphorylatable MRLC show a delay in ...
Steinberger Simon - - 2011
Novel A-D-A type oligothiophenes incorporating benzothiadiazole (BTDA) and thiadiazolopyridine (TDAPy) as terminal acceptor groups have been developed for small molecule organic solar cells (SMSC). In vacuum-processed planar heterojunction solar cells the TDAPy-based oligomer showed a power conversion efficiency of 3.15% and a high fill factor of 0.67.
Kopf Phillip G PG Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, - - 2011
Elevated concentrations of aldosterone are associated with several cardiovascular diseases. Angiotensin II (Ang II) increases aldosterone secretion and adrenal blood flow. This concurrent increase in steroidogenesis and adrenal blood flow is not understood. We investigated the role of zona glomerulosa (ZG) cells in the regulation of vascular tone of bovine ...
Rodrigues Marcoaurélio Almenara - - 2011
The cell wall of Chlorella is composed of up to 80% carbohydrates including cellulose. In this study, Chlorella homosphaera and Chlorella zofingiensis were evaluated as source of fermentable sugars via their cell wall enzymatic degradation. The algae were cultivated in inorganic medium, collected at the stationary growth phase and centrifuged. ...
Egerod Kristoffer L - - 2011
Mice deficient in the zinc-sensor GPR39, which has been demonstrated to protect cells against endoplasmatic stress and cell death in vitro, display moderate glucose intolerance and impaired glucose-induced insulin secretion. Here, we use the Tet-On system under the control of the proinsulin promoter to selectively overexpress GPR39 in the β ...
Chen Jin-Shuen - - 2011
The aim of this study is to investigate the role of chaperonin-containing t-complex polypeptide 1 beta (CCT2) in the regulation of mouse mesangial cell (mMC) contraction, proliferation, and migration with filamentous/globular-(F/G-) actin ratio under high glucose induction. A low CCT2 mMC model induced by treatment of small interference RNA was established. ...
Sargsyan Ernest - - 2011
Prolonged elevated levels of lipids have negative effects on beta-cell function and mass (lipotoxicity). To what extent exposure to high glucose concentration is important in the harmful effects of lipids (glucolipotoxicity) has been debated. We addressed beta-cell lipotoxicity by measuring apoptosis in isolated intact control human islets and insulin-secreting cell ...
Meehan Terrence F TF Mouse Genome Informatics, The Jackson Laboratory, Bar Harbor, ME, USA. - - 2011
The Cell Ontology (CL) is an ontology for the representation of in vivo cell types. As biological ontologies such as the CL grow in complexity, they become increasingly difficult to use and maintain. By making the information in the ontology computable, we can use automated reasoners to detect errors and ...
Jacobsen Faith E FE Department of Chemistry, Indiana University, Bloomington, IN 47405-7102, - - 2011
ICP-MS analysis of Streptococcus pneumoniae reveals a high cell-associated Mn(II) concentration that is comparable to that of Zn(II). Stressing these cells with 100–200 μM Zn(II) leads to a slow-growth phenotype and a total Mn(II) concentration that is reduced, with no decrease of other metal ions. Supplementation of the growth media ...
Poon Song Ling - - 2011
GnRH-II enhances ovarian cancer cell invasion in an autocrine manner. We have now found that GnRH-II increases 37-kDa laminin receptor precursor (LRP) production in GnRH receptor (GnRHR)-positive OVCAR-3 and CaOV-3 ovarian cancer cells, while small interfering RNA (siRNA)-mediated depletion of GnRH-II or GnRHR mRNA abrogates this. The invasiveness of ovarian ...
Fu Sheng - - 2010
J Oral Pathol Med (2010) Bone morphogenetic proteins (BMPs), one of the crucial regulators in embryonic development and bone formation, have been implicated in epithelium-derived tumors. Previous results showed the involvement of overexpression of BMP 2, 4, 5 in the carcinogenesis of oral epithelia. The ability of BMP receptor-II mutant ...
Inoue-Kashino Natsuko - - 2010
Sll1252 was identified as a novel protein in photosystem II complexes from Synechocystis sp. PCC 6803. To investigate the function of Sll1252, the corresponding gene, sll1252, was deleted in Synechocystis 6803. Despite the homology of Sll1252 to YlmH, which functions in the cell division machinery in Streptococcus, the growth rate ...
Charest Pascale G PG University of California, San Diego, La Jolla, 92093-0380, - - 2010
During cell migration, chemoattractant-induced signaling pathways determine the direction of movement by controlling the spatiotemporal dynamics of cytoskeletal components. In this issue of Developmental Cell, Liu et al. report that the target of rapamycin complex 2 (TORC2) controls cell polarity and chemotaxis through regulation of both F-actin and myosin II in ...
Burraston Bert O - - 2012
The purpose of this research was to evaluate the effects of a cognitive training and cell phone intervention on the recidivism of 70 juvenile offenders. Median days to rearrest were 106 for the control group, 191 for the class-only group, and 278 for the class plus cell phone group. Using ...
Lan Shao-Yang - - 2010
Msi1 (Musashi 1) is regarded as a marker for neural and intestinal epithelial stem cells. However, it is still unclear whether Msi1-positive cells derived from mouse embryonic stem cells have the ability to differentiate into neural or intestinal epithelial cells. A pMsi1-GFP (green fluorescent protein) reporter plasmid was constructed in ...
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