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Gunkel Manuel M BioQuant, ViroQuant-CellNetworks RNAi Screening Facility, Ruprecht-Karls-Universitat Heidelberg, Im Neuenheimer Feld 267, Heidelberg, 69120, - - 2015
Using RNAi interference (RNAi), it is possible to study the effect of specific gene knockdowns in mammalian cells. In this protocol we present the automated preparation of "ready to transfect" multiwell plates and cell arrays, on which cells can be grown which are then reversely transfected with one type of ...
Worden N N Department of Plant Sciences, University of California Davis, One Shields Avenue, Davis, CA, 95616, - - 2015
The cell wall is directly involved in cell growth, and its ability to loosen and rearrange allows for cell expansion through the existing turgor pressure. Thus, information on cell wall deposition and rearrangement can provide insights into the overall plant growth. This chapter describes two methods that can be used ...
Park Guen Tae GT Department of Biological Sciences, Seoul National University, Seoul 151-747, - - 2014
Angiosperm reproduction is characterized by alternate diploid sporophytic and haploid gametophytic generations. Gametogenesis shares similarities with that of animals except for the formation of the gametophyte, whereby haploid cells undergo several rounds of postmeiotic mitosis to form gametes and the accessory cells required for successful reproduction. The mechanisms regulating gametophyte ...
Fitzgerald Valerie - - 2014
The interrogation of highly diverse repertoires of heterogeneous cell populations on a single cell basis increases the likelihood that a cell with unique characteristics will be identified. We have developed a new single cell analysis system comprising millions of bundled sub nanoliter volume bio-incubation chambers for the identification and recovery ...
Giampaolo Bosi B Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, St. Trentacoste 2, 20134 Milan, - - 2014
Intestinal mucous cell numbers and their glycoconjugate composition were investigated by histochemical methods in uninfected chub, Squalius cephalus, and in conspecifics naturally parasitised with the acanthocephalan Pomphorhynchus laevis. A sub-population of 42 chub from the River Tiber (Perugia, Italy) were sampled and screened for ecto and endoparasites. No parasites were ...
Adrichem Arjan J van - - 2014
The Rho family of Ras superfamily small GTPases regulates a broad range of biological processes such as migration, differentiation, cell growth and cell survival. Therefore, the availability of small molecule modulators as tool compounds could greatly enhance research on these proteins and their biological function. To this end, we designed ...
Chang Lingqian L NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH, 43209, USA; Biomedical Engineering Department, Ohio State University, Columbus, OH, 43209, - - 2014
A novel high-throughput magnetic tweezers-based 3D microchannel electroporation system capable of transfecting 40 000 cells/cm(2) on a single chip for gene therapy, regenerative medicine, and intracellular detection of target mRNA for screening cellular heterogeneity is reported. A single cell or an ordered array of individual cells are remotely guided by ...
Suzuki Hirobumi H Department of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan. Electronic address: - - 2014
Small bowel adenocarcinoma (SBA) is a rare, aggressive malignancy with a poor prognosis, and the mechanisms of carcinogenesis in SBA remain unclear. Our aims were to investigate the molecular mechanisms underlying SBA and to identify treatments by establishing and characterizing an SBA cell line and performing anti-cancer drug screening. SIAC1 ...
Martorana Annamaria A Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche "STEBICEF" - Università di Palermo, Via Archirafi 32, 90123 Palermo, - - 2014
A new series of 3-benzoylamino-5-imidazol-5-yl-benzo[b]thiophenes and the parent amino derivatives were synthesized and screened as antitumor agents. All tested compounds showed concentration-dependent antiproliferative activity profile against HeLa cell line, exhibiting GI50 values in the low micromolar range. The most active compounds were tested in cell cycle perturbation experiments. A rapid ...
Luzina Elena L EL Institute of Physiologically Active Compounds, Severnyi pr. 1, Chernogolovka, Moscow region, 142432, - - 2014
Novel trifluoromethylated mono- and bicyclic succinimides derived from trifluoromethylmaleic anhydride were synthesized using cyclopentadiene or 2,3-dimethylbutadiene and (het)arylamines. The biological activity of these compounds was evaluated using prediction methods and experimental studies. This series of new trifluoromethyl succinimides (3a,b and 6a-c) were tested by the National Cancer Institute (NCI, Bethesda, ...
Tosso Perrer N - - 2014
EWS-FLI1 is an oncogenic fusion protein implicated in the development of Ewing sarcoma family tumors (ES). Using our previously reported lead compound 2 (YK-4-279), we designed and synthesized a focused library of analogues. The functional inhibition of the analogues were measured by an EWS-FLI1/ NR0B1 reporter luciferase assay and a ...
Hou Jin J School of Pharmaceutical Sciences, Xiamen University, Xiang'an South Road, Xiamen, 361102, P.R. - - 2014
In this study, a series of novel N-(piperidine-4-yl)benzamide derivatives was designed, synthesized, and evaluated for antitumor activity. Some compounds were found to have potent antitumor activity. In particular, compound 47 showed the most potent biological activity against HepG2 cells, with an IC50 value of 0.25 μM. Western blot analysis demonstrated ...
Li Peiwu P Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. - - 2014
Colorectal cancer (CRC) is a common gastrointestinal malignancy worldwide and is a lethal and aggressive malignancy with a dismal prognosis. EphrinB2 is a membrane-bound ligand and has an intracellular domain that also possesses an intrinsic signaling capacity called 'reverse signaling'. In the present study, CRC cell lines were screened for ...
Bradshaw Michael J MJ School of Biological Sciences, University of Northern Colorado, 501 20th St., CB 92, Greeley, CO, 80639-0017, - - 2014
Snake venoms are mixtures of bioactive proteins and peptides that exhibit diverse biochemical activities. This wide array of pharmacologies associated with snake venoms has made them attractive sources for research into potentially novel therapeutics, and several venom-derived drugs are now in use. In the current study we performed a broad ...
Petti Consalvo C Candiolo Cancer Institute - FPO IRCCS, - - 2014
Constitutively active receptor tyrosine kinases (RTKs) are known oncogenic drivers and provide valuable therapeutic targets in many cancer types. However, clinical efficacy of RTK inhibitors is limited by intrinsic and acquired resistance. To identify genes conferring resistance to inhibition of the MET RTK, we conducted a forward genetics screen in ...
Rizwan-Ul-Haq Muhammad M Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Hofuf, Saudi Arabia, - - 2014
Cell cultures can be a potent and strong tool to evaluate the insecticidal efficiency of natural products. Plant essential oils have long been used as the fragrance or curative products around the world which means that they are safer to be used in close proximity of humans and mammals. In ...
Tanay Das Mihir - - 2014
Wide spread use of Di-(2-ethylhexyl) phthalate (DEHP) has made it a ubiquitous contaminant in today's environment, responsible for possible carcinogenic and endocrine disrupting effects. In the present investigation an integrative toxicoproteomic approach was made to study the estrogenic potential of DEHP. In vitro experiments carried out with DEHP (0.1-100 microM) ...
Yamaguchi Miki M Department of Molecular Medicine, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Hokkaido 060-8556, Japan. Electronic address: - - 2014
Antibody-drug conjugates (ADCs), drugs developed by conjugation of an anticancer agent to a monoclonal antibody (mAb), have lately attracted attention in cancer therapy because ADCs can directly bind cancer cells and kill them. Although mAbs for ADCs must be internalized by the target cells, few methods are available for screening ...
Zhang Li-Sha - - 2014
A novel hollow fiber cell fishing with high performance liquid chromatography (HFCF-HPLC) was extended and used to screen flavonoid and anthraquinone active compound groups simultaneously from traditional Chinese medicines (TCMs). In this study, three cells (MCF-7, SGC7901, and MADB-106) were seeded on the inner wall of the hollow fiber employed ...
He Yang-Yang YY Beijing Key Laboratory of Drug Targets Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, - - 2014
Aim:Blockade of interleukin-6 (IL-6) or its receptor (IL-6R) is effective in preventing the progression of autoimmune diseases, such as systemic lupus erythematosus and rheumatoid arthritis. In the present study, we established a novel cell-based assay for identifying small molecule IL-6R antagonists.Methods:HEK293A cells were transfected with recombinant plasmids pTaglite-SNAP-IL6R and pABhFc-IL6 ...
Szalóki György G Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, 48 Vas. Constantinou Avenue, Athens 11635, - - 2014
A series of novel C21-alkynylaryl derivatives of pregnenolone were synthesized and screened for anticancer activity against a panel of seven human cancer cell lines (LNCaP, A549, MCF7, HeLa, A431, HepG2, HT29). The data revealed that these compounds can be potential antitumour agents against the specific cell models. Compound 6f bearing ...
Zheng Xiushan X State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, 710032, - - 2014
Uncontrolled proliferation is a key characteristic of gastric carcinogenesis and the precise mechanisms underlying the altered proliferation behaviors of GC cells have not been clearly elucidated. miRNAs has been suggested to play a crucial role in the pathogenesis and development of various cancers. In the present study, we employed an ...
Huang Xiao-Chao XC State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Yucai Road 15, Guilin 541004, Guangxi, PR - - 2014
A series of novel dehydroabietic acid (DHA) chiral dipeptide derivatives were designed and synthesized as potent antitumor agents. The inhibitory activities of these compounds against NCI-H460 (lung), HeLa (epithelial cervical) and MGC-803 (gastric) human cancer cell lines were estimated by MTT assay in vitro. The antitumor activities screening indicated that many ...
Sunassee Suthananda N SN Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702-1201, United - - 2014
Bioinformatic analysis of data from the NCI-60 cell cytotoxicity screen revealed a subset of extracts that showed selective cytotoxic activity toward human colon carcinoma cell lines. Bioassay-guided fractionation of a colon cancer selective extract from a Philippines collection of the marine sponge Corticium niger provided two new steroidal alkaloids, plakinamines ...
Gurdal Enise Ece EE Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yeditepe University , Kayisdagi, Istanbul , Turkey - - 2014
Abstract Synthesis, characterization and cytotoxic activities of ten benzothiazole-piperazine derivatives were reported. In vitro cytotoxic activities of compounds were screened against hepatocellular (HUH-7), breast (MCF-7) and colorectal (HCT-116) cancer cell lines by sulphorhodamine B assay. Based on the GI50 values of the compounds, most of the benzothiazole-piperazine derivatives are active ...
Diana Patrizia - - 2014
Derivatives of new ring systems 11H-pyrido[3',2':4,5]pyrrolo[3,2-c]cinnoline and pyrido[3',2':4,5]pyrrolo[1,2-c][1,2,3]benzotriazine were prepared from the key intermediates 2-(1H-pyrrolo[2,3-b]pyridin-2-yl)anilines in excellent yields (94-99%) and screened by the National Cancer Institute (NCI, Bethesda, USA), on about 60 human tumor cell lines derived from nine cancer cell types. Tested compounds exhibited antiproliferative activity against all the ...
Baumeister Leslie L Department of Marine Sciences, Texas A&M University, Galveston Campus, 200 Seawolf Parkway, Galveston, Texas 77553, - - 2014
Dorsal and pectoral fin spines from two species of sea catfishes (Bagre marinus and Ariopsis felis) landed at 54 sites in Galveston Bay, Texas, and its subbays from June to October 2005 were screened with traditional cultivation-based assays and quantitative PCR assays for Vibrio vulnificus and Vibrio parahaemolyticus. V. vulnificus ...
Kheirollahi Asma A Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, - - 2014
With the aim of discovering potential cytotoxic agents, a series of benzochromene derivatives were screened for their cytotoxic activity against seven human cancer cell lines by standard 3-(4, 5-dimethyl thiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptosis, as the mechanism of cell death, was investigated morphologically by acridine orange/ethidium bromide staining and ...
Jani Charul C Department of Biochemistry and Biomedical Sciences, Michael DeGroote Institute for Infectious Diseases Research, McMaster University, Hamilton, ON, - - 2014
Cell division is essential for spore formation but not for viability in the filamentous streptomycetes bacteria. Failure to complete cell division instead blocks spore formation, a phenotype that can be visualized by the absence of gray (in Streptomyces coelicolor) and green (in Streptomyces venezuelae) spore-associated pigmentation. Despite the lack of ...
Peter Stefanie S Theodor Boveri Institute, Biocenter, University of Würzburg, Würzburg, - - 2014
Deregulated expression of MYC is a driver of colorectal carcinogenesis, necessitating novel strategies to inhibit MYC function. The ubiquitin ligase HUWE1 (HECTH9, ARF-BP1, MULE) associates with both MYC and the MYC-associated protein MIZ1. We show here that HUWE1 is required for growth of colorectal cancer cells in culture and in ...
Gi Mia M Department of Otorhinolaryngology, Brain Korea 21 PLUS Project for Medical - - 2014
Pseudomonas aeruginosa, a Gram-negative bacterium of clinical significance, produces elastase as a predominant exoprotease. Here, we screened a library of chemical compounds currently used for human medication and identified diethylene triamine pentaacetic acid (DTPA, pentetic acid) as an agent that suppresses the production of elastase. Elastase activity found in the ...
Wang Peng-Yuan PY Department of Chemical Engineering, National Taiwan University, No.1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan; Industrial Research Institute Swinburne (IRIS), and Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, 3122 VIC, Australia; CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, 3168 VIC, - - 2014
It is well known that the surface chemistry of biomaterials is important both for initial cell attachment and the downstream cell response. Surface chemistry gradients are a new format that allows the screening of the subtleties of cell-surface interactions in high-throughput. In this study, two surface chemical gradients were fabricated ...
Cha Junghwa J Department of Bio and Brain Engineering, KAIST, Daejeon, 305-701, - - 2014
Understanding the effects of topographic characteristics on tumor cell migration is important for the development of new anti-migratory therapies. However, simplified in vitro culture systems often lead to inaccurate results regarding the efficacy of drugs. Histopathologically, glioblastoma multiform (GBM) cells migrate along the orientation of thin, elongated anatomical structures, such ...
Sahu Saura C SC Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, - - 2014
Two widely used in vitro cell culture models, human liver HepG2 cells and human colon Caco2 cells, and flow cytometry techniques were evaluated as tools for rapid screening of potential genotoxicity of food-related nanosilver. Comparative genotoxic potential of 20 nm silver was evaluated in HepG2 and Caco2 cell cultures by a ...
Kagoya Yuki Y Department of Hematology & Oncology, Graduate School of Medicine, The University of Tokyo, Tokyo, - - 2014
Genetic instability is strongly involved in cancer development and progression, and elucidating the mechanism could lead to novel therapeutics for preventing carcinogenesis. Philadelphia-negative myeloproliferative neoplasms (MPN) are clonal myeloid disorders with high prevalence of JAK2V617F mutation, and its transformation to acute myeloid leukemia through accumulation of additional mutations is a ...
Zou Ling L Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, - - 2014
Paralytic shellfish poisoning (PSP) toxins are well-known sodium channel-blocking marine toxins, which block the conduction of nerve impulses and lead to a series of neurological disorders symptoms. However, PSP toxins can inhibit the cytotoxicity effect of compounds (e.g., ouabain and veratridine). Under the treatment of ouabain and veratridine, neuroblastoma cell ...
Richter Julia - - 2014
Deregulation of CK1 (Casein Kinase 1) activity can be involved in the development of several pathological disorders and diseases such as cancer. Therefore, research interest in identifying potent CK1-specific inhibitors is still increasing. A previously published potent and selective benzimidazole-derived CK1δ/ε-specific inhibitor compound with significant effects on several tumor cell ...
Tan Yuyu - - 2014
Separating the specific from the nonspecific bound single strand DNA (ssDNA) is the most important step to improve the efficiency of selection procedure. However, most cell-SELEX protocols use simply washing only, which leads to incomplete separation. It is well established that ssDNAs can be adsorbed on single-walled carbon nanotubes (SWCNTs). ...
Shelton Jadd R JR Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, United - - 2014
Efficient methods for the preparation of 5'-substituted 5'-amino-5'-deoxy-N(6)-ureidoadenosine derivatives are described. Compounds were screened for antiproliferative activity against a panel of murine and human cell lines (L1210, CEM, and HeLa) and/or against the NCI-60. The most potent derivative inhibited the lung adenocarcinoma cell line NCI-H522 at low nanomolar concentrations (GI50=9.7nM).
Robert Francis F Departments of - - 2014
Enhanced protein synthesis capacity is associated with increased tumor cell survival, proliferation, and resistance to chemotherapy. Cancers like multiple myeloma (MM), which display elevated activity in key translation regulatory nodes, such as the PI3K/mammalian target of rapamycin and MYC-eukaryotic initiation factor (eIF) 4E pathways, are predicted to be particularly sensitive ...
Corcoran Jacob A JA School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New - - 2014
The development of rapid and reliable assays to characterize insect odorant receptors (ORs) and pheromone receptors (PRs) remains a challenge for the field. Typically ORs and PRs are functionally characterized either in vivo in transgenic Drosophila or in vitro through expression in Xenopus oocytes. While these approaches have succeeded, they ...
Shaul Yoav D YD Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA; Koch Institute for Integrative Cancer Research at MIT, 77 Massachusetts Avenue, Cambridge, MA 02139, - - 2014
It is increasingly appreciated that oncogenic transformation alters cellular metabolism to facilitate cell proliferation, but less is known about the metabolic changes that promote cancer cell aggressiveness. Here, we analyzed metabolic gene expression in cancer cell lines and found that a set of high-grade carcinoma lines expressing mesenchymal markers share ...
Chan Manuel M Rosalind and Morris Goodman Cancer Research Centre, McGill - - 2014
GMX1778 and its pro-drug GMX1777 represent a new class of cancer drugs that target nicotinamide phosphoribosyltransferase (NAMPT), as a new strategy to interfere with biosynthesis of the key enzymatic co-factor NAD, which is critical for a number of cell functions including DNA repair. Using a genome-wide synthetic lethal siRNA screen, ...
Sharma Simmi S Natural Product Microbes, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-Tawi 180001, J&K, - - 2014
The synthesis and bio-evaluation of naturally occurring boswellic acids (BAs) as an alternate CAP for the design of new HDAC inhibitors is described. All the compounds were screened against a panel of human cancer cell lines to identify leads, which were subsequently examined for their potential to inhibit HDACs. The ...
Wong Charng Choon CC Pharmacotherapeutics Unit, Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400 Selangor, - - 2014
Andrographolide (AGP) is the main bioactive constituent isolated from the traditional medicinal, Andrographis paniculata which contributes towards its various biological activities, including anticancer property. In this study, a series of new AGP derivatives were semi-synthesised and screened against the NCI in vitro 60 cell lines. From the screening results, we ...
Muylaert Dimitri Ep DE Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, The - - 2014
Synthetic replacement grafts for heart valves and small-diameter blood vessels such as coronary arteries have the potential to circumvent many of the limitations of currently available autologous grafting materials. Cell-free material incorporating biologically active compounds may guide the formation of fully autologous new tissue in situ derived from host cells ...
Lisch Walter W *Department of Ophthalmology, Johannes Gutenberg University Mainz, Mainz, Germany; and †Department of Ophthalmology, University Medical Center UKS, Saarland, - - 2014
To evaluate the question whether lattice corneal dystrophy type 1 (LCD1) is of epithelial or stromal origin. The landmark of advanced LCD1 shows central superficial haze and paracentral stromal lattice lines. In 16 eyes of 8 affected individuals of 2 families, a penetrating keratoplasty was performed. The follow-up was 8 ...
Kayabasoglu Gurkan G Department of Otorhinolaryngology, Faculty of Medicine, Sakarya University, Adnan Menderes Cad. No 145, Adapazar─▒, Sakarya, 54100, Turkey, - - 2014
To compare the cellular viability of diced, crushed, and morselized cartilage used in nasal surgeries. In this study, cartilage was extracted from the ears of seven New Zealand rabbits and was subsequently either diced, crushed or morselized to an amorphous state, or left unmodified. The four types of grafts were ...
Kweon HaeYong H Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-853, Republic of - - 2014
Grafted macromolecules often induce granuloma formation with foreign body giant cell (FBGC) infiltration, and this is the main reason for graft failure. Diacylglycerol kinase (DAGK) is an important intracellular mediator of FBGC formation in macrophages. In this study, 4-hexylresorcinol (4HR) inhibited DAGKδ in a macrophage cell line (RAW264.7 cells). As ...
Cui Haitao - - 2014
In this study, a novel electroactive tetreaniline-containing degradable polyelectrolyte multilayer film (PEM) coating [(poly(L-glutamic acid)-graft-tetreaniline/poly(L-lysine)-graft-tetreaniline)n, (PGA-g-TA/PLL-g-TA)n] was designed and fabricated by layer-by-layer (LbL) assembly method. Compared with the nongrafted PEMs, the tetreaniline-grafted PEMs showed higher roughness and stiffness in micro/nanoscale structures. The special surface characteristics and the typical electroconductive properties ...
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