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Kheirollahi Asma A Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, - - 2014
With the aim of discovering potential cytotoxic agents, a series of benzochromene derivatives were screened for their cytotoxic activity against seven human cancer cell lines by standard 3-(4, 5-dimethyl thiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptosis, as the mechanism of cell death, was investigated morphologically by acridine orange/ethidium bromide staining and ...
Jani Charul C Department of Biochemistry and Biomedical Sciences, Michael DeGroote Institute for Infectious Diseases Research, McMaster University, Hamilton, ON, - - 2014
Cell division is essential for spore formation but not for viability in the filamentous streptomycetes bacteria. Failure to complete cell division instead blocks spore formation, a phenotype that can be visualized by the absence of gray (in Streptomyces coelicolor) and green (in Streptomyces venezuelae) spore-associated pigmentation. Despite the lack of ...
Peter Stefanie S Theodor Boveri Institute, Biocenter, University of Würzburg, Würzburg, - - 2014
Deregulated expression of MYC is a driver of colorectal carcinogenesis, necessitating novel strategies to inhibit MYC function. The ubiquitin ligase HUWE1 (HECTH9, ARF-BP1, MULE) associates with both MYC and the MYC-associated protein MIZ1. We show here that HUWE1 is required for growth of colorectal cancer cells in culture and in ...
Gi Mia M Department of Otorhinolaryngology, Brain Korea 21 PLUS Project for Medical - - 2014
Pseudomonas aeruginosa, a Gram-negative bacterium of clinical significance, produces elastase as a predominant exoprotease. Here, we screened a library of chemical compounds currently used for human medication and identified diethylene triamine pentaacetic acid (DTPA, pentetic acid) as an agent that suppresses the production of elastase. Elastase activity found in the ...
Wang Peng-Yuan PY Department of Chemical Engineering, National Taiwan University, No.1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan; Industrial Research Institute Swinburne (IRIS), and Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, 3122 VIC, Australia; CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, 3168 VIC, - - 2014
It is well known that the surface chemistry of biomaterials is important both for initial cell attachment and the downstream cell response. Surface chemistry gradients are a new format that allows the screening of the subtleties of cell-surface interactions in high-throughput. In this study, two surface chemical gradients were fabricated ...
Cha Junghwa J Department of Bio and Brain Engineering, KAIST, Daejeon, 305-701, - - 2014
Understanding the effects of topographic characteristics on tumor cell migration is important for the development of new anti-migratory therapies. However, simplified in vitro culture systems often lead to inaccurate results regarding the efficacy of drugs. Histopathologically, glioblastoma multiform (GBM) cells migrate along the orientation of thin, elongated anatomical structures, such ...
Sahu Saura C SC Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD, 20708, - - 2014
Two widely used in vitro cell culture models, human liver HepG2 cells and human colon Caco2 cells, and flow cytometry techniques were evaluated as tools for rapid screening of potential genotoxicity of food-related nanosilver. Comparative genotoxic potential of 20 nm silver was evaluated in HepG2 and Caco2 cell cultures by a ...
Kagoya Yuki Y Department of Hematology & Oncology, Graduate School of Medicine, The University of Tokyo, Tokyo, - - 2014
Genetic instability is strongly involved in cancer development and progression, and elucidating the mechanism could lead to novel therapeutics for preventing carcinogenesis. Philadelphia-negative myeloproliferative neoplasms (MPN) are clonal myeloid disorders with high prevalence of JAK2V617F mutation, and its transformation to acute myeloid leukemia through accumulation of additional mutations is a ...
Zou Ling L Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, - - 2014
Paralytic shellfish poisoning (PSP) toxins are well-known sodium channel-blocking marine toxins, which block the conduction of nerve impulses and lead to a series of neurological disorders symptoms. However, PSP toxins can inhibit the cytotoxicity effect of compounds (e.g., ouabain and veratridine). Under the treatment of ouabain and veratridine, neuroblastoma cell ...
Richter Julia - - 2014
Deregulation of CK1 (Casein Kinase 1) activity can be involved in the development of several pathological disorders and diseases such as cancer. Therefore, research interest in identifying potent CK1-specific inhibitors is still increasing. A previously published potent and selective benzimidazole-derived CK1δ/ε-specific inhibitor compound with significant effects on several tumor cell ...
Tan Yuyu - - 2014
Separating the specific from the nonspecific bound single strand DNA (ssDNA) is the most important step to improve the efficiency of selection procedure. However, most cell-SELEX protocols use simply washing only, which leads to incomplete separation. It is well established that ssDNAs can be adsorbed on single-walled carbon nanotubes (SWCNTs). ...
Shelton Jadd R JR Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, United - - 2014
Efficient methods for the preparation of 5'-substituted 5'-amino-5'-deoxy-N(6)-ureidoadenosine derivatives are described. Compounds were screened for antiproliferative activity against a panel of murine and human cell lines (L1210, CEM, and HeLa) and/or against the NCI-60. The most potent derivative inhibited the lung adenocarcinoma cell line NCI-H522 at low nanomolar concentrations (GI50=9.7nM).
Robert Francis F Departments of - - 2014
Enhanced protein synthesis capacity is associated with increased tumor cell survival, proliferation, and resistance to chemotherapy. Cancers like multiple myeloma (MM), which display elevated activity in key translation regulatory nodes, such as the PI3K/mammalian target of rapamycin and MYC-eukaryotic initiation factor (eIF) 4E pathways, are predicted to be particularly sensitive ...
Shaul Yoav D YD Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA; Koch Institute for Integrative Cancer Research at MIT, 77 Massachusetts Avenue, Cambridge, MA 02139, - - 2014
It is increasingly appreciated that oncogenic transformation alters cellular metabolism to facilitate cell proliferation, but less is known about the metabolic changes that promote cancer cell aggressiveness. Here, we analyzed metabolic gene expression in cancer cell lines and found that a set of high-grade carcinoma lines expressing mesenchymal markers share ...
Corcoran Jacob A JA School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New - - 2014
The development of rapid and reliable assays to characterize insect odorant receptors (ORs) and pheromone receptors (PRs) remains a challenge for the field. Typically ORs and PRs are functionally characterized either in vivo in transgenic Drosophila or in vitro through expression in Xenopus oocytes. While these approaches have succeeded, they ...
Chan Manuel M Rosalind and Morris Goodman Cancer Research Centre, McGill - - 2014
GMX1778 and its pro-drug GMX1777 represent a new class of cancer drugs that target nicotinamide phosphoribosyltransferase (NAMPT), as a new strategy to interfere with biosynthesis of the key enzymatic co-factor NAD, which is critical for a number of cell functions including DNA repair. Using a genome-wide synthetic lethal siRNA screen, ...
Sharma Simmi S Natural Product Microbes, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-Tawi 180001, J&K, - - 2014
The synthesis and bio-evaluation of naturally occurring boswellic acids (BAs) as an alternate CAP for the design of new HDAC inhibitors is described. All the compounds were screened against a panel of human cancer cell lines to identify leads, which were subsequently examined for their potential to inhibit HDACs. The ...
Wong Charng Choon CC Pharmacotherapeutics Unit, Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400 Selangor, - - 2014
Andrographolide (AGP) is the main bioactive constituent isolated from the traditional medicinal, Andrographis paniculata which contributes towards its various biological activities, including anticancer property. In this study, a series of new AGP derivatives were semi-synthesised and screened against the NCI in vitro 60 cell lines. From the screening results, we ...
Muylaert Dimitri Ep DE Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, The - - 2014
Synthetic replacement grafts for heart valves and small-diameter blood vessels such as coronary arteries have the potential to circumvent many of the limitations of currently available autologous grafting materials. Cell-free material incorporating biologically active compounds may guide the formation of fully autologous new tissue in situ derived from host cells ...
Lisch Walter W *Department of Ophthalmology, Johannes Gutenberg University Mainz, Mainz, Germany; and †Department of Ophthalmology, University Medical Center UKS, Saarland, - - 2014
To evaluate the question whether lattice corneal dystrophy type 1 (LCD1) is of epithelial or stromal origin. The landmark of advanced LCD1 shows central superficial haze and paracentral stromal lattice lines. In 16 eyes of 8 affected individuals of 2 families, a penetrating keratoplasty was performed. The follow-up was 8 ...
Kayabasoglu Gurkan G Department of Otorhinolaryngology, Faculty of Medicine, Sakarya University, Adnan Menderes Cad. No 145, Adapazar─▒, Sakarya, 54100, Turkey, - - 2014
To compare the cellular viability of diced, crushed, and morselized cartilage used in nasal surgeries. In this study, cartilage was extracted from the ears of seven New Zealand rabbits and was subsequently either diced, crushed or morselized to an amorphous state, or left unmodified. The four types of grafts were ...
Kweon HaeYong H Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Suwon 441-853, Republic of - - 2014
Grafted macromolecules often induce granuloma formation with foreign body giant cell (FBGC) infiltration, and this is the main reason for graft failure. Diacylglycerol kinase (DAGK) is an important intracellular mediator of FBGC formation in macrophages. In this study, 4-hexylresorcinol (4HR) inhibited DAGKδ in a macrophage cell line (RAW264.7 cells). As ...
Cui Haitao - - 2014
In this study, a novel electroactive tetreaniline-containing degradable polyelectrolyte multilayer film (PEM) coating [(poly(L-glutamic acid)-graft-tetreaniline/poly(L-lysine)-graft-tetreaniline)n, (PGA-g-TA/PLL-g-TA)n] was designed and fabricated by layer-by-layer (LbL) assembly method. Compared with the nongrafted PEMs, the tetreaniline-grafted PEMs showed higher roughness and stiffness in micro/nanoscale structures. The special surface characteristics and the typical electroconductive properties ...
Roslen Nurfariza Ahmad NA Department of Biomedical Science, Kulliyyah of Allied Health Sciences, IIUM, 25200 Kuantan, Pahang, - - 2014
To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro. A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts ...
Kustiawan Paula M PM Program in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand ; Department of Forest Product Technology, Faculty of Forestry, Mulawarman University, Samarinda, 75123, - - 2014
To screen crude extracts of propolis, bee pollen and honey from four stingless bee species [Trigona incisa (T. incisa)], Timia apicalis, Trigona fusco-balteata and Trigona fuscibasis) native to East Kalimantan, Indonesia for cytotoxic activity against five human cancer cell lines (HepG2, SW620, ChaGo-I, KATO-III and BT474). All samples were extracted ...
Mendelson Avital A New York, N.Y. From the Center for Craniofacial Regeneration and the Department of Otolaryngology-Head and Neck Surgery, Columbia University Medical Center; and the Department of Biomedical Engineering, Columbia - - 2014
Current augmentative and reconstructive rhinoplasties use auto logous tissue grafts or synthetic bioinert materials to repair nasal trauma or attain an aesthetic shape. Autologous grafts are associated with donor-site trauma and morbidity. Synthetic materials are widely used but often yield an unnatural appearance and are prone to infection or dislocation. ...
Felgueiras Helena H Laboratory of Biomaterials and Specialty Polymers, LBPS-CSPBAT UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 Avenue Jean-Baptiste Clément, 93430 Villetaneuse, - - 2014
Ten years ago, we synthesized "bioactive model polymers" bearing sulfonate groups and proposed a mechanism of their modulation effect at different steps of the cell response. Then, we set up the grafting of polymers bearing sulfonate on Ti6Al4V surfaces by a grafting "from" technique making sure of the creation of ...
Cabane Etienne E Wood Materials Science, Institute for Building Materials, ETH Zürich, Stefano-Franscini-Platz 3, 8093 Zürich (Switzerland); Applied Wood Materials, EMPA-Swiss Federal Laboratories for Materials Science and Technology, Überlandstrasse 129, 8600 Dübendorf (Switzerland). - - 2014
A "grafting-from" polymerization approach within and at the complex and heterogeneous macromolecular assembly of wood cell walls is shown. The approach allows for the implementation of novel functionalities in renewable and functional wood-based materials. The native wood structure is retained and used as a hierarchical multiscale framework for a modular ...
Anguissola Sergio S Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, - - 2014
The fast-paced development of nanotechnology needs the support of effective safety testing. We have developed a screening platform measuring simultaneously several cellular parameters for exposure to various concentrations of nanoparticles (NPs). Cell lines representative of different organ cell types, including lung, endothelium, liver, kidney, macrophages, glia, and neuronal cells were ...
Costa Roberta - - 2013
Although intracerebral transplantation of various fibroblast (-like) cell populations has been shown feasible, little is known about the actual in vivo remodelling of these cellular grafts and their environment. In this study, we aimed to compare the in vitro and in vivo behaviour of two phenotypically similar ? but developmentally ...
Vélez Christian C Universidad Metropolitana, San Juan, Puerto - - 2013
This study reports the capacity of three nitro substituted benzazolo[3,2-a]quinolinium salts NBQs: NBQ 95 (NSC-763304), NBQ 38 (NSC 763305), and NBQ 97 (NSC-763306) as potential antitumor agents. NBQ's are unnatural alkaloids possessing a positive charge that could facilitate interaction with cell organelles. The anticancer activities of these compounds were evaluated ...
Knapik Alicia - - 2013
Storage of split-thickness skin grafts (STSGs) represents a standard procedure in burn surgery. The purpose of this study was to evaluate clinical routine of STSG preservation. Further, we aimed at investigating the effect of storage on tissue integrity and cell viability, proliferation, apoptosis and vascularization. A survey was performed among ...
Chung Tze-Wen - - 2013
Poly(ε-caprolactone) (PCL) scaffolds functionalized by grafting nerve growth factor (NGF) and Asp-Arg-Gly-Asp (GRGD)(PCL-NGF/GRGD) for neural tissue engineering. The influences of PCL-NGF/GRGD scaffolds on the growth and differentiation of PC12 cells were investigated. The successfully grafting NGF and GRGD into PCL-CS scaffold were verified by FTIR spectra. The densities of GRGD ...
Williams Stuart K - - 2013
We evaluated the use of an automated, point-of-care instrument to derive canine adipose stromal vascular fraction cells, and the subsequent deposition of these cells onto the luminal surface of an expanded polytetrafluoroethylene (ePTFE) vascular graft for use as a bypass graft. The hypothesis evaluated was that an instrument requiring minimal ...
Alam N - - 2013
We investigated the cellular biology of tendon grafting in a mouse model using green fluorescent protein mismatch grafting and quantitative immunohistochemistry of molecular markers for inflammation, proliferation, collagen synthesis, cell death, and myofibroblast/pericyte expression. We provide a detailed analysis of the healing characteristics during the phases of inflammation, synthesis, and ...
Hohlrieder M - - 2013
Various studies have shown that physical stimuli modulate cell function and this has motivated the development of a bioreactor to engineer tissues in vitro by exposing them to mechanical loads. Here, we present a bioreactor for the physical stimulation of anterior cruciate ligament (ACL) grafts, whereby complex multi-dimensional strain can ...
Sys Gwen M L - - 2013
Sarcoma is a very rare disease that is heterogeneous in nature, all hampering the development of new therapies. Sarcoma patients are ideal candidates for personalized medicine after stratification, explaining the current interest in developing a reproducible and low-cost xenotransplant model for this disease. The chick chorioallantoic membrane is a natural ...
Gimi Barjor B Department of Radiology, Dartmouth Medical School, Hanover, New Hampshire; Department of Medicine, Dartmouth Medical School, Hanover, New - - 2013
Rapid developments in the therapeutic applications of genetically engineered cells and stem cell research have increased the possibilities of addressing some pathologies by grafting therapeutic cells. Immunoprotective encapsulation of such therapeutic cells is often essential for their survival and function. Hydrogels provide a bioteolerable matrix for cellular encapsulation and support ...
Bergmeister Helga - - 2012
Pore size and porosity control rate and depth of cellular migration in electrospun vascular fabrics and thereby have a strong impact on long-term graft success. In this study we investigated the effect of graft porosity on cell migration in-vitro and in-vivo. Polyetherurethane (PU) grafts were fabricated by electrospinning as fine ...
Hayashi Masanori M Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 1st Street S.W., Rochester, MN 55905 - - 2012
We have recently reported that interpositional synovium grafts from tendon sheath have a potential to accelerate tendon healing when implanted at the repair site. The purpose of this study was to investigate the effect of orientation of the synovium after synovium graft transplantation, by comparing the ability of cells from ...
Panneerselvan Anitha - - 2012
An implantable scaffold pre-seeded with cells needs to remain viable and encourage rapid angiogenesis in order to replace injured tissues, especially for tissue defect repairs. We created a bioartificial adipose graft composed of an electrospun 3D nanofibrous scaffold and fat tissue excised from New Zealand white rabbits. Cell viability and ...
Hartmann-Fritsch Fabienne - - 2012
PURPOSE: Human amniotic fluid comprises cells with high differentiation capacity, thus representing a potential cell source for skin tissue engineering. In this experimental study, we investigated the ability of human amniotic fluid derived cells to substitute dermal fibroblasts and support epidermis formation and stratification in a humanized animal model. METHODS: ...
Chitta Kasyapa S KS Department of Hematology and Oncology, Mayo Clinic, Jacksonville, FL 32224, USA. - - 2013
Understanding the biology of Waldenström macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal ...
Kasuga Jun - - 2012
The supercooling capability of xylem parenchyma cells (XPCs) in boreal hardwood species differs depending not only on species but also season. In the present study, the roles of cell walls and intracellular contents in supercooling capability of XPCs were examined in three boreal hardwood species, Japanese beech, katsura tree and ...
Delgado Dolores - - 2012
Stomata are epidermal bi-celled structures that differentiate within special cell lineages initiated by a subset of protodermal cells. Recently, we showed that the Arabidopsis photomorphogenic repressor COP10 controls specific cell-lineage and cell-signaling developmental mechanisms in stomatal lineages. Loss-of-function cop10-1 mutant cotyledons and leaves produced (in the light and in the ...
Li Guocan - - 2012
We report the first case of Ru(ii) dipyrrinates employed as dyes in dye-sensitized solar cells. These complexes exhibit panchromatic light harvesting that results in significant DSSC current densities, rendering them promising for photovoltaic applications. Adjustment of the lowest excited state energy is required to boost the power conversion efficiency.
Sumaru Kimio - - 2012
As a powerful tool of cell screening and cell purification, we developed a novel method to kill adherent cells as cultured on a substrate by micro-projection of incoherent visible light. To kill the cells by the mild light irradiated by electrically controllable micro-projection systems currently available, we introduced the assist ...
Hernández-Franyutti Arlette - - 2012
We describe seasonal variations of the histology of the seminiferous tubules and efferent ducts of the tropical, viviparous skink, Mabuya brachypoda, throughout the year. The specimens were collected monthly, in Nacajuca, Tabasco state, Mexico. The results revealed strong annual variations in testicular volume, stages of the germ cells, and diameter ...
Berk Veysel - - 2012
In their natural environment, microbes organize into communities held together by an extracellular matrix composed of polysaccharides and proteins. We developed an in vivo labeling strategy to allow the extracellular matrix of developing biofilms to be visualized with conventional and superresolution light microscopy. Vibrio cholerae biofilms displayed three distinct levels ...
Sun Yubing Y Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, United - - 2012
Mechanical properties of the extracellular matrix (ECM) have profound effects on cellular functions. Here, we applied novel photosensitive polydimethylsiloxane (photoPDMS) chemistry to create photosensitive, biocompatible photoPDMS as a rigidity-tunable material for study of mechanoresponsive cellular behaviors. By modulating the PDMS cross-linker to monomer ratio, UV light exposure time, and postexposure ...
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