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Roslen Nurfariza Ahmad NA Department of Biomedical Science, Kulliyyah of Allied Health Sciences, IIUM, 25200 Kuantan, Pahang, - - 2014
To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro. A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts ...
Kustiawan Paula M PM Program in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand ; Department of Forest Product Technology, Faculty of Forestry, Mulawarman University, Samarinda, 75123, - - 2014
To screen crude extracts of propolis, bee pollen and honey from four stingless bee species [Trigona incisa (T. incisa)], Timia apicalis, Trigona fusco-balteata and Trigona fuscibasis) native to East Kalimantan, Indonesia for cytotoxic activity against five human cancer cell lines (HepG2, SW620, ChaGo-I, KATO-III and BT474). All samples were extracted ...
Ostafe Raluca - - 2014
A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to ...
Mendelson Avital A New York, N.Y. From the Center for Craniofacial Regeneration and the Department of Otolaryngology-Head and Neck Surgery, Columbia University Medical Center; and the Department of Biomedical Engineering, Columbia - - 2014
Current augmentative and reconstructive rhinoplasties use auto logous tissue grafts or synthetic bioinert materials to repair nasal trauma or attain an aesthetic shape. Autologous grafts are associated with donor-site trauma and morbidity. Synthetic materials are widely used but often yield an unnatural appearance and are prone to infection or dislocation. ...
Felgueiras Helena H Laboratory of Biomaterials and Specialty Polymers, LBPS-CSPBAT UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 Avenue Jean-Baptiste Clément, 93430 Villetaneuse, - - 2014
Ten years ago, we synthesized "bioactive model polymers" bearing sulfonate groups and proposed a mechanism of their modulation effect at different steps of the cell response. Then, we set up the grafting of polymers bearing sulfonate on Ti6Al4V surfaces by a grafting "from" technique making sure of the creation of ...
Cabane Etienne E Wood Materials Science, Institute for Building Materials, ETH Zürich, Stefano-Franscini-Platz 3, 8093 Zürich (Switzerland); Applied Wood Materials, EMPA-Swiss Federal Laboratories for Materials Science and Technology, Überlandstrasse 129, 8600 Dübendorf (Switzerland). - - 2014
A "grafting-from" polymerization approach within and at the complex and heterogeneous macromolecular assembly of wood cell walls is shown. The approach allows for the implementation of novel functionalities in renewable and functional wood-based materials. The native wood structure is retained and used as a hierarchical multiscale framework for a modular ...
Anguissola Sergio S Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, - - 2014
The fast-paced development of nanotechnology needs the support of effective safety testing. We have developed a screening platform measuring simultaneously several cellular parameters for exposure to various concentrations of nanoparticles (NPs). Cell lines representative of different organ cell types, including lung, endothelium, liver, kidney, macrophages, glia, and neuronal cells were ...
Ranade Aarati R AR Molecular and Cell Biology Systems, EMD Millipore Corporation, 290 Concord Road, Billerica, MA 01821, - - 2014
High-throughput imaging-based hepatotoxicity studies capable of analyzing individual cells in situ hold enormous promise for drug safety testing but are frequently limited by a lack of sufficient metabolically competent human cells. This study examined cryopreserved HepaRG cells, a human liver cell line which differentiates into both hepatocytes and biliary epithelial ...
Liberio Michelle S MS Eskitis Institute for Drug Discovery, Griffith University, Nathan, Qld 4111, Australia. - - 2014
Ascidians are marine invertebrates that have been a source of numerous cytotoxic compounds. Of the first six marine-derived drugs that made anticancer clinical trials, three originated from ascidian specimens. In order to identify new anti-neoplastic compounds, an ascidian extract library (143 samples) was generated and screened in MDA-MB-231 breast cancer ...
Arora Shagun S Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh 173234, - - 2014
Achyranthes aspera (AA) has been used traditionally for the cure of various disorders. However, the action of root extracts of AA as anticancer agent and its cellular mechanism remain unclear. The aim was to screen the antitumor effect of ethanolic (EAA) and aqueous (AAA) root extracts on the growth of ...
Althubiti M M 1] Department of Biochemistry, University of Leicester, Leicester, UK [2] Department of Biochemistry, Faculty of Medicine, Umm AL-Qura University, Mecca, Saudi - - 2014
Cellular senescence is a terminal differentiation state that has been proposed to have a role in both tumour suppression and ageing. This view is supported by the fact that accumulation of senescent cells can be observed in response to oncogenic stress as well as a result of normal organismal ageing. ...
Bagchi Bhattacharjee Gargi G 0000-0001-6416-2584 Amity Institute of Biotechnology, Amity University, Amity Education Valley, Panchgaon, Gurgaon, Manesar, Haryana 122413, - - 2014
Endocrine disruptive chemicals (EDCs) modulate hormone signaling and cause developmental and reproductive anomalies. Today, there is a global concern regarding endocrine disruption effects, particularly those mediated by the androgen receptor (AR). Androgen or male hormones are critical for the development and maintenance of male characteristics and numerous EDCs exist in ...
Costa Roberta - - 2013
Although intracerebral transplantation of various fibroblast (-like) cell populations has been shown feasible, little is known about the actual in vivo remodelling of these cellular grafts and their environment. In this study, we aimed to compare the in vitro and in vivo behaviour of two phenotypically similar ? but developmentally ...
Vélez Christian C Universidad Metropolitana, San Juan, Puerto - - 2013
This study reports the capacity of three nitro substituted benzazolo[3,2-a]quinolinium salts NBQs: NBQ 95 (NSC-763304), NBQ 38 (NSC 763305), and NBQ 97 (NSC-763306) as potential antitumor agents. NBQ's are unnatural alkaloids possessing a positive charge that could facilitate interaction with cell organelles. The anticancer activities of these compounds were evaluated ...
Knapik Alicia - - 2013
Storage of split-thickness skin grafts (STSGs) represents a standard procedure in burn surgery. The purpose of this study was to evaluate clinical routine of STSG preservation. Further, we aimed at investigating the effect of storage on tissue integrity and cell viability, proliferation, apoptosis and vascularization. A survey was performed among ...
Chung Tze-Wen - - 2013
Poly(ε-caprolactone) (PCL) scaffolds functionalized by grafting nerve growth factor (NGF) and Asp-Arg-Gly-Asp (GRGD)(PCL-NGF/GRGD) for neural tissue engineering. The influences of PCL-NGF/GRGD scaffolds on the growth and differentiation of PC12 cells were investigated. The successfully grafting NGF and GRGD into PCL-CS scaffold were verified by FTIR spectra. The densities of GRGD ...
Williams Stuart K - - 2013
We evaluated the use of an automated, point-of-care instrument to derive canine adipose stromal vascular fraction cells, and the subsequent deposition of these cells onto the luminal surface of an expanded polytetrafluoroethylene (ePTFE) vascular graft for use as a bypass graft. The hypothesis evaluated was that an instrument requiring minimal ...
Alam N - - 2013
We investigated the cellular biology of tendon grafting in a mouse model using green fluorescent protein mismatch grafting and quantitative immunohistochemistry of molecular markers for inflammation, proliferation, collagen synthesis, cell death, and myofibroblast/pericyte expression. We provide a detailed analysis of the healing characteristics during the phases of inflammation, synthesis, and ...
Hohlrieder M - - 2013
Various studies have shown that physical stimuli modulate cell function and this has motivated the development of a bioreactor to engineer tissues in vitro by exposing them to mechanical loads. Here, we present a bioreactor for the physical stimulation of anterior cruciate ligament (ACL) grafts, whereby complex multi-dimensional strain can ...
Sys Gwen M L - - 2013
Sarcoma is a very rare disease that is heterogeneous in nature, all hampering the development of new therapies. Sarcoma patients are ideal candidates for personalized medicine after stratification, explaining the current interest in developing a reproducible and low-cost xenotransplant model for this disease. The chick chorioallantoic membrane is a natural ...
Gimi Barjor B Department of Radiology, Dartmouth Medical School, Hanover, New Hampshire; Department of Medicine, Dartmouth Medical School, Hanover, New - - 2013
Rapid developments in the therapeutic applications of genetically engineered cells and stem cell research have increased the possibilities of addressing some pathologies by grafting therapeutic cells. Immunoprotective encapsulation of such therapeutic cells is often essential for their survival and function. Hydrogels provide a bioteolerable matrix for cellular encapsulation and support ...
Bergmeister Helga - - 2012
Pore size and porosity control rate and depth of cellular migration in electrospun vascular fabrics and thereby have a strong impact on long-term graft success. In this study we investigated the effect of graft porosity on cell migration in-vitro and in-vivo. Polyetherurethane (PU) grafts were fabricated by electrospinning as fine ...
Hayashi Masanori M Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 1st Street S.W., Rochester, MN 55905 - - 2012
We have recently reported that interpositional synovium grafts from tendon sheath have a potential to accelerate tendon healing when implanted at the repair site. The purpose of this study was to investigate the effect of orientation of the synovium after synovium graft transplantation, by comparing the ability of cells from ...
Panneerselvan Anitha - - 2012
An implantable scaffold pre-seeded with cells needs to remain viable and encourage rapid angiogenesis in order to replace injured tissues, especially for tissue defect repairs. We created a bioartificial adipose graft composed of an electrospun 3D nanofibrous scaffold and fat tissue excised from New Zealand white rabbits. Cell viability and ...
Hartmann-Fritsch Fabienne - - 2012
PURPOSE: Human amniotic fluid comprises cells with high differentiation capacity, thus representing a potential cell source for skin tissue engineering. In this experimental study, we investigated the ability of human amniotic fluid derived cells to substitute dermal fibroblasts and support epidermis formation and stratification in a humanized animal model. METHODS: ...
Chitta Kasyapa S KS Department of Hematology and Oncology, Mayo Clinic, Jacksonville, FL 32224, USA. - - 2013
Understanding the biology of Waldenström macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal ...
Kasuga Jun - - 2012
The supercooling capability of xylem parenchyma cells (XPCs) in boreal hardwood species differs depending not only on species but also season. In the present study, the roles of cell walls and intracellular contents in supercooling capability of XPCs were examined in three boreal hardwood species, Japanese beech, katsura tree and ...
Delgado Dolores - - 2012
Stomata are epidermal bi-celled structures that differentiate within special cell lineages initiated by a subset of protodermal cells. Recently, we showed that the Arabidopsis photomorphogenic repressor COP10 controls specific cell-lineage and cell-signaling developmental mechanisms in stomatal lineages. Loss-of-function cop10-1 mutant cotyledons and leaves produced (in the light and in the ...
Li Guocan - - 2012
We report the first case of Ru(ii) dipyrrinates employed as dyes in dye-sensitized solar cells. These complexes exhibit panchromatic light harvesting that results in significant DSSC current densities, rendering them promising for photovoltaic applications. Adjustment of the lowest excited state energy is required to boost the power conversion efficiency.
Sumaru Kimio - - 2012
As a powerful tool of cell screening and cell purification, we developed a novel method to kill adherent cells as cultured on a substrate by micro-projection of incoherent visible light. To kill the cells by the mild light irradiated by electrically controllable micro-projection systems currently available, we introduced the assist ...
Hernández-Franyutti Arlette - - 2012
We describe seasonal variations of the histology of the seminiferous tubules and efferent ducts of the tropical, viviparous skink, Mabuya brachypoda, throughout the year. The specimens were collected monthly, in Nacajuca, Tabasco state, Mexico. The results revealed strong annual variations in testicular volume, stages of the germ cells, and diameter ...
Berk Veysel - - 2012
In their natural environment, microbes organize into communities held together by an extracellular matrix composed of polysaccharides and proteins. We developed an in vivo labeling strategy to allow the extracellular matrix of developing biofilms to be visualized with conventional and superresolution light microscopy. Vibrio cholerae biofilms displayed three distinct levels ...
Sun Yubing Y Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, United - - 2012
Mechanical properties of the extracellular matrix (ECM) have profound effects on cellular functions. Here, we applied novel photosensitive polydimethylsiloxane (photoPDMS) chemistry to create photosensitive, biocompatible photoPDMS as a rigidity-tunable material for study of mechanoresponsive cellular behaviors. By modulating the PDMS cross-linker to monomer ratio, UV light exposure time, and postexposure ...
Johnson Alyssa E AE Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, - - 2012
Mitotic exit and cell division must be spatially and temporally integrated to facilitate equal division of genetic material between daughter cells. In the fission yeast, Schizosaccharomyces pombe, a spindle pole body (SPB) localized signaling cascade termed the septation initiation network (SIN) couples mitotic exit with cytokinesis. The SIN is controlled ...
Lim Wonbong - - 2012
Abstract Objective: The aim of this study was to examine the reactive oxygen species (ROS) that are dissipated by 635 nm irradiation, and the effect of 635 nm irradiation on ROS scavenging system. Background data: Intracellular ROS are produced in the form of superoxide anion by either nicotinamide adenine dinucleotide phosphate (NADPH) ...
Xiao Jian-Long - - 2012
We used a liquid-crystal spatial light modulator to project 473 nm light patterns surrounding a region of adherent cells and achieved an arbitrarily micropatterned cell culture. For a group of ∼60 cells, the cell boundaries fit the pattern of light within 15% deviation of the side length. We also demonstrated ...
Kim Chanhong - - 2012
Enhanced levels of singlet oxygen ((1)O(2)) in chloroplasts trigger programmed cell death. The impact of (1)O(2) production in chloroplasts was monitored first in the conditional fluorescent (flu) mutant of Arabidopsis thaliana that accumulates (1)O(2) upon a dark/light shift. The onset of (1)O(2) production is rapidly followed by a loss of ...
Victora Gabriel D - - 2012
Germinal centers (GCs) are sites of B-cell clonal expansion, hypermutation, and selection. GCs are polarized into dark (DZ) and light zones (LZ), a distinction that is of key importance to GC selection. However, the difference between the B cells in each of these zones in humans remains unclear. We show ...
Nomikou Nikolitsa - - 2012
Photodynamic therapy (PDT) is emerging as a treatment modality for the management of neoplastic disease. Despite considerable clinical success, its application for the treatment of deep-seated lesions is constrained by the inability of visible light to penetrate deeply into tissues. An emerging alternative approach exploits the fact that many photosensitisers ...
Westendorf Aron F - - 2012
Photoactivatable PtIV diazido complexes have unusual photobiological properties. We demonstrate here that trans,trans,trans-[PtIV(N3)2(OH)2(py)(NH3)] 3 is a potent photoactivated cytotoxin towards human cancer cells in culture, with an average IC50 value in 13 cell lines of 55 ± 28 µM after 30 min (0.12 mW/cm2) photoactivation with UVA light, although visible ...
Singh Shailendra P - - 2012
Fremyella diplosiphon modifies the pigment composition of its major light-harvesting complexes, i.e., phycobilisomes (PBS), and cell and filament morphology according to ambient light quality in a process termed complementary chromatic adaptation (CCA). The cells are red in color, rectangular-shaped, and filaments are longer under green light (GL), in contrast with ...
Miyake Takeo - - 2012
Similar to conventional electrolyte batteries, biofuel cells often need to be stacked in order to boost their single cell voltage (<1V) up to a practical level. Here, we report a laminated stack of biofuel cells that is composed of bioanode fabrics for fructose oxidation, hydrogel sheets containing electrolyte and fuel ...
Mishra Swati - - 2012
Hydrogels, containing yeast cells or fibroblast cells, were fabricated using blue light-induced polymerization technique. The cell-loaded prepolymer formulation was comprised of poly(ethyleneglycol) diacrylate (more than or equal to 50% v/v), 0.5 wt % Eosin Y and 0.5 wt % triethanolamine as the base oligomer, photo-initiator, and co-initiator, respectively. The two ...
Sheng Xia - - 2012
The intraovarian function of gonadally produced inhibin and activin has been extensively studied in experimental models for decades, yet their presence and function have been rarely reported in wild rodents. With our seasonal breeding model, the wild ground squirrel, we aimed to investigate the possible roles of these peptides in ...
Itoh Masahiko - - 2012
The organization of the apical junctional complex and its association with the cytoskeleton is essential for the function of epithelial cells. However, knowledge about the signaling pathways that regulate these processes is still fragmentary. Here we found that ARHGEF11, a member of the RGS-RhoGEF family, associates with tight junctions (TJs) ...
Tamai T Katherine - - 2012
A fundamental role of the circadian clock is to control biochemical and physiological processes such that they occur an optimal time of day. One of the most significant clock outputs from a clinical as well as basic biological standpoint is the timing of the cell cycle. Here we show that ...
Gallardo J J - - 2012
This paper presents a non-conventional methodology and an instrumental system to measure the effect of temperature on the photovoltaic properties of solar cells. The system enables the direct measurement of the evolution of open-circuit voltage and short-circuit current intensity in relation to a continuously decreasing temperature. The system uses a ...
Yang Qing - - 2012
An integrated system consisting of a carbon fiber-ZnO hybrid nanowire (NW) multicolor photodetector is driven by a microbial fuel cell (see picture; PMMA=poly(methyl methacrylate), E=electrode). The self-powered photodetector can detect at light levels of as little as nW cm(-2) intensity with a responsivity of more than 300 A W(-1) .
Fidopiastis Pat M - - 2012
Vibrio fischeri cells are the sole colonists of a specialized light organ in the mantle cavity of the sepiolid squid Euprymna scolopes. The process begins when the bacteria aggregate in mucus secretions outside the light organ. The cells eventually leave the aggregate, enter the light organ, and encounter a rich ...
Zhang Yunlin - - 2012
We investigated phytoplankton absorption properties of Lake Taihu, in the spring and summer of 2005 and 2006, and for 17 days studied laboratory cultures of Scenedesmus obliquus (chlorophyta) and Microcystis aeruginosa (cyanophyta) to determine the effect of phytoplankton community composition and cell size on the absorption properties. There were significant ...
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