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Ong S-B - - 2014
The mechanism through which the protein kinase Akt (also called PKB), protects the heart against acute ischaemia-reperfusion injury (IRI) is not clear. Here, we investigate whether Akt mediates its cardioprotective effect by modulating mitochondrial morphology. Transfection of HL-1 cardiac cells with constitutively active Akt (caAkt) changed mitochondrial morphology as evidenced ...
Glowacki Andrew J AJ Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, - - 2014
Disease and injury perturb the balance of processes associated with inflammation and tissue remodeling, resulting in positive feedback loops, exacerbation of disease and compromised tissue repair. Conversely, under homeostatic healthy conditions, these processes are tightly regulated through the expansion and/or recruitment of specific cell populations, promoting a balanced steady-state. Better ...
Linck Guimarães Eduardo E Liver Cell Biology lab, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, 1090, - - 2014
Liver fibrosis is induced by the accumulation of extracellular matrix, deposited mainly by activated hepatic stellate cells (HSCs). One key characteristic of stellate cell activation is the directional migration to the site of injury during the wound-healing process. P311 is a protein that has been shown to play a role ...
Mikuła-Pietrasik Justyna J Laboratory of Gerontology, Department of Pathophysiology, These authors contributed equally to this - - 2014
There is agreement that the biological properties of additionally hydroxylated analogs of resveratrol (RVT) may be stronger compared with their parent drug. Here we examined the effect of a novel RVT derivative, 3,3',4,4'-tetrahydroxy-trans-stilbene (3,3',4,4'-THS), on the replicative senescence of human peritoneal mesothelial cells. The study showed that 3,3',4,4'-THS improves the ...
Qin Yu Y Cadet Brigade, Third Military Medical University, Chongqing, People's Republic of - - 2014
New neurons are continuously generated throughout life in the subgranular zone in the dentate gyrus of the mammalian hippocampus and in the subventricular zone of the lateral ventricles. With the aid of new methodologies, significant progress has been made in the characterization of endogenous stem cells (ependymal cells) and their ...
Brechbuhl Heather M HM National Jewish - - 2014
Rational: ERB family receptors (EGFR, ERB-B2, ERB-B3, and ERB-B4) regulate epithelial cell function in many tissue types. In the human airway epithelium, changes in ERB receptor expression are associated with epithelial repair defects. However, the specific role(s) played by ERB receptors in repair have not been determined. Objective: To determine ...
Zhao Jun J Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, 1555 Holcombe Blvd., Houston, TX 77030, - - 2014
The treatment of liver injuries or hepatocellular carcinoma (HCC) has been hindered by the lack of efficient drug delivery. Even with the help of nanoparticles or other synthetic delivering agents, a large portion of the dose is still sequestered in the reticuloendothelial system. As an alternative, adipose-derived mesenchymal cells (AD-MSCs), ...
Higuita-Castro Natalia N The Ohio State - - 2014
Interfacial flows during cyclic airway reopening are an important source of ventilator induced lung injury. However, it is not known how changes in airway wall compliance influence cell injury during airway reopening. We used an in-vitro model of airway reopening in a compliant micro-channel to investigate how airway wall stiffness ...
Macho-Fernandez E E Trudeau Institute, Saranac Lake, New York, - - 2014
The immune mechanisms regulating epithelial cell repair after injury remain poorly defined. We demonstrate here that lymphotoxin beta receptor (LTβR) signaling in intestinal epithelial cells promotes self-repair after mucosal damage. Using a conditional gene-targeted approach, we demonstrate that LTβR signaling in intestinal epithelial cells is essential for epithelial interleukin-23 (IL-23) ...
Casey C A CA Internal Medicine, University of Nebraska Medical Center, - - 2014
Alcohol-induced alterations in cell function, hepatic inflammation, and fibrosis are prominent features of liver disease in general and of alcoholic liver injury in particular. The link between these processes, however, remains unclear. A virtually universal characteristic of liver injury and subsequent inflammation is the induction of hepatocellular damage, and work ...
Kramann Rafael R Brigham and Women's Hospital, Boston, MA, USA Harvard Medical School, Boston, MA, USA Division of Nephrology, RWTH Aachen University, Aachen, - - 2014
The kidney possesses profound regenerative potential and in some cases can recover completely 'restitutio at integrum' following an acute kidney injury (AKI). Emerging evidence strongly suggests that sometimes repair is incomplete, however, and, in this situation, an episode of AKI leads to future chronic kidney disease (CKD). Understanding the tubular ...
Liu Peng P Department of Hepatobiliary Surgery, The Navy General Hospital, Beijing, 100048, People's Republic of - - 2014
To investigate the effects of icaritin, an active ingredient extracted from Epimedium Sagittatum (Sieb. et Zucc.), on CCl4-induced liver injury and its possible mechanisms. Hepatocytes isolated from Sprague-Dawley male rats were treated with 3 mmol/L CCl4 for 24 h to induce acute liver cell injury, then icaritin (0.1, 1, 10, 100 μmol/L, respectively) ...
Liu Jo-Wen JW Center For Health Disparities and Molecular Medicine, Loma Linda University School of Medicine, Loma Linda, CA, - - 2014
Epidermal-fatty acid binding protein (E-FABP/ FABP5/DA11) binds and transport long-chain fatty acids in the cytoplasm and may play a protecting role during neuronal injury. We examined whether E-FABP protects nerve growth factor differentiated PC12 cells (NGFDPC12 cells) from lipotoxic injury observed after palmitic acid (C16:0; PAM) overload. NGFDPC12 cells cultures ...
Masterson Claire - - 2014
Rojas and colleagues have presented an exciting paper demonstrating yet another relevant preclinical setting in which the mesenchymal stromal cell has a potential therapeutic application. What is particularly interesting about this study is that it addresses a disease, blood-borne systemic sepsis, which has multiple complex host responses and involves a ...
Berger Katja K Division of Nephrology and Clinical Immunology, Rheinisch-Westfaelische Technische Hochschule (RWTH), Aachen, - - 2014
Acute kidney injury (AKI) is a common clinical problem and is associated with high mortality rates. It is accepted that after AKI cellular regeneration of the proximal tubule occurs from intrinsic tubule cells. Recently, scattered tubular cells (STCs) were discovered as a novel subpopulation of tubule cells involved in regeneration. ...
Mohammadi Tamimount T Membrane Biochemistry and Biophysics, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht (The - - 2014
Getting visible: A new method to label bacterial cell walls shows the presence of functional peptidoglycan in the important pathogen Chlamydia trachomatis. This might clarify the long-standing paradox of the "chlamydial anomaly".
Yamazaki Tomohiro T Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan. Electronic address: - - 2014
Chlamydia trachomatis is an obligate intracellular bacterium that causes a sexually transmitted disease. Ureaplasma parvum is commensal in the human genital tract, with a minimal contribution to urogenital infection. We have recently found that U. parvum has a significant effect on the presence of C. trachomatis in the genital tract of healthy ...
Abdanipour Alireza A Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, - - 2014
To evaluate the effects of glial cell line-derived neurotrophic factor transplantation combined with adipose-derived stem cells-transdifferentiated motoneuron delivery on spinal cord contusion injury, we developed rat models of spinal cord contusion injury, 7 days later, injected adipose-derived stem cells-transdifferentiated motoneurons into the epicenter, rostral and caudal regions of the impact ...
Frandi Antonio A Department of Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva, Rue Michel Servet 1, 1211 Genève 4, - - 2014
The nature and assembly of the chlamydial division septum is poorly defined due to the paucity of a detectable peptidoglycan (PG)-based cell wall, the inhibition of constriction by penicillin and the presence of coding sequences for cell wall precursor and remodelling enzymes in the reduced chlamydial (pan-)genome. Here we show ...
Qian Weidong W Department of Cardiology, Traditional Chinese Medicine Hospital of Wujin District, Affiliated Hospital of Nanjing University of Chinese Medicine, Changzhou 213161, - - 2014
Myocardial ischemia-reperfusion injury (MIRI) is a common pathological and physiological phenomenon. Tetramethylpyrazine is the extract of the traditional Chinese medicine Chuanxiong, which can exert protective effects on MIRI in multiple ways. This paper reviewed the current research progress and evidence about the cardiovascular effects of tetramethylpyrazine, which included protecting mitochondria ...
Fernandes Ana Carolina Morais AC Universidade Federal de São Paulo, São Paulo, SP, - - 2014
To propose an experimental burn model in NIH-3T3 cell line. Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence. The heating of the Petri dish increased proportionally to the temperature of the base and the time of ...
Liechti G W GW 1] Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4799, USA - - 2014
Peptidoglycan (PG), an essential structure in the cell walls of the vast majority of bacteria, is critical for division and maintaining cell shape and hydrostatic pressure. Bacteria comprising the Chlamydiales were thought to be one of the few exceptions. Chlamydia harbour genes for PG biosynthesis and exhibit susceptibility to 'anti-PG' ...
Yu Xiuling X Department of Ophthalmology, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, - - 2013
A plasmid for cytoglobin expression, pAcGFP1-C1-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-1α in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible factor-1α expression ...
Gloeckl Sarina - - 2013
The mechanistic details of the pathogenesis of Chlamydia, an obligate intracellular pathogen of global importance, have eluded scientists due to the scarcity of traditional molecular genetic tools to investigate this organism. Here we report a chemical biology strategy that has uncovered the first essential protease for this organism. Identification and ...
Zhang Shu-Xin SX Spinal Cord Society Research Center, Fort Collins, CO 80526, - - 2013
Schwann cells are glial cells of peripheral nervous system, responsible for axonal myelination and ensheathing, as well as tissue repair following a peripheral nervous system injury. They are one of several cell types that are widely studied and most commonly used for cell transplantation to treat spinal cord injury, due ...
Moore Elizabeth R - - 2012
Chlamydia trachomatis is an obligate intracellular human pathogen, which lacks a system that allows genetic manipulation. Therefore, chlamydial researchers must manipulate the host cell to better understand chlamydial biology. Host-derived lipid acquisition is critical for chlamydial survival within the host. Hence, the ability to track and purify sphingolipids in/from chlamydial ...
Wenderska Iwona B - - 2012
In Streptococcus mutans, ComX, an alternative sigma factor, drives the transcription of the 'late-competence genes' required for genetic transformation. ComX activity is modulated by inputs from two signaling pathways, ComDE and ComRS, that respond to the competence-stimulating peptide (CSP) and the SigX-inducing peptide (XIP), respectively. In particular, the comRS, encoding ...
Zhai Chunmei - - 2012
Quorum sensing (QS) is a system of cell-to-cell communication by means of intercellular signalling molecules to coordinate a set of targeted gene expression or repression in many Gram-negative bacteria; it plays important roles for bacteria in adaptation to adverse environmental conditions. In this study, we first demonstrated that Microcystis aeruginosa PCC-7820 ...
Lamanna Jason J - - 2012
: Clinical trials using stem cells to treat Amyotrophic Lateral Sclerosis (ALS) have been promising. However, most of these trials lack an appropriate approach for tracking the cells. The goal of the present study is to develop and validate a rapidly translatable methodology for in vivo visualization and post-mortem identification ...
Toubi Elias - - 2012
Laquinimod is an orally administered drug under development for the treatment of Multiple Sclerosis (MS), lacking a fully elucidated mode of action. We assessed the immunomodulatory effects of laquinimod in vitro on human B cells from healthy or MS patients, cultured alone or with CD4(+) T cells. Laquinimod modulated B ...
Barkla Bronwyn J - - 2012
Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been proposed to have roles ...
Ferrauto Giuseppe - - 2012
Conventional T(1) - or T(2) -MRI contrast agents do not allow to track the distribution of different cell populations simultaneously because the effects of relaxation enhancers are additive. Herein, it is shown that paramagnetic chemical exchange saturation transfer agents offer the opportunity to visualize different cell populations in vitro and ...
Mahjoub Moe R MR Department of Biology, Stanford University, Stanford, CA 94305, - - 2012
The primary cilium is a nexus of cell signaling, and ciliary dysfunction is associated with polycystic kidney disease, retinal degeneration, polydactyly, neural tube defects, and obesity (ciliopathies). Signaling molecules for cilium-associated pathways are concentrated in the cilium, and this is essential for efficient signaling. Cilia are nucleated from centrioles, and ...
Everest-Dass Arun V - - 2012
Mucosal epithelial surfaces, such as line the oral cavity, are common sites of microbial colonization by bacteria, yeast and fungi. The microbial interactions involve adherence between the glycans on the host cells and the carbohydrate binding proteins of the pathogen. Saliva constantly bathes the buccal cells of the epithelial surface ...
Bell Katharina - - 2012
BACKGROUND: Many contact lens wearers suffer from dry eye syndrome. Previous studies show significant changes in the protein composition of the tears depending on the lens-cleaning solution used. Therefore, the aim of this study was to detect the influence of different lens solutions on conjunctival cells, cells also involved in ...
Holien Toril - - 2012
In multiple myeloma, c-MYC is activated and contributes to the malignant phenotype. Targeting c-MYC by short hairpin RNA induced cell death in myeloma cell lines however; cell lines are generated from samples taken in advanced stages of the disease and may not reflect patient cells adequately. In this study we ...
Zhang Li L Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6084, - - 2012
Polycyclic aromatic hydrocarbons (PAH) are environmental and tobacco carcinogens. Human aldo-keto reductases catalyze the metabolic activation of proximate carcinogenic PAH trans-dihydrodiols to yield electrophilic and redox-active o-quinones. Benzo[a]pyrene-7,8-dione a representative PAH o-quinone is reduced back to the corresponding catechol to generate a futile redox-cycle. We investigated whether sulfonation of PAH ...
Tep Samnang - - 2012
Within the biotechnology industry there is a continuous drive to better and more fully understand the biopharmaceutical process in order to achieve better process control. A method to monitor and quantitate glycomic changes that occur in CHO cells during a bioreactor campaign could help to address this. The goal of ...
Xu Jing - - 2012
Three new iridoids, jatairidoids A-C (1-3, resp.), have been isolated from the roots of Valeriana jatamansi (V. wallichii). Their structures were elucidated by spectroscopic methods (IR, ESI-MS, HR-ESI-MS, 1D- and 2D-NMR). Compounds 1 and 2 are C(3)-epimers. The three compounds were evaluated for their neuroprotective effects against MPP(+) -induced neuronal ...
Davis Kimberley J - - 2012
The cytotoxicity of the metallointercalators, [Pt(5,6-dimethyl-1,10-phenanthroline)(trans-1R,2R-diaminocyclohexane)](2+) ([56MERR]) and [Pt(5,6-dimethyl-1,10-phenanthroline)(trans-1S,2S-diaminocyclohexane)](2+) ([56MESS]), towards A549 human lung cancer cells was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The IC(50) value obtained following exposure of A549 cells to [56MESS] for 4 h was approximately three times smaller than that obtained when [56MERR] was administered ...
Sahab Ziad Z Department of Pathology, Georgetown University Medical School, Washington, DC, - - 2012
The high-risk human papillomavirus type 16 (HPV-16) E5 protein (16E5) induces tumors in a transgenic mouse model and may contribute to early stages of cervical carcinogenesis. Although high-risk E5 expression is generally thought to be lost during the progression to cervical carcinoma following integration of HPV DNA into the host ...
Vasquez M I - - 2012
Ofloxacin (OFL), a broad-spectrum and widespread-used photolabile fluoroquinolone, is frequently found in treated wastewaters, aquatic and terrestrial ecosystems leading to increasing concern during the past decades regarding its effects to the environment and human health. The elimination of OFL and other xenobiotics by the application of advanced oxidation processes using ...
Chen Xufeng - - 2012
Histone deacetylase inhibitors (HDIs) have shown promise as candidate radiosensitizers for many types of cancers. However, the mechanisms of action are not well understood, and whether they could sensitize multiple myeloma (MM) to radiation therapy is unclear. In this study, we demonstrate that Suberoylanilide hydroxamic acid (SAHA) at low concentrations ...
Lahaye Marc - - 2012
Cell wall composition, pectin and hemicellulose fine structure variation were assessed in peach and related genotypes with contrasted texture and fruit shape. Cell walls were prepared from four commercial peaches, eight genotypes from the Jalousia x Fantasia peach cross, and six genotypes from the Earlygold peach x Texas almond cross. ...
Campbell Graham R GR The Mitochondrial Research Group, Institute for Ageing and Health, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, - - 2012
Mitochondrial DNA deletions (∆-mtDNA) have been implicated in the pathogenesis of Alzheimer's disease (AD), multiple sclerosis (MS) and Parkinson's disease (PD), as well as ageing. Clonal expansion of ∆-mtDNA is the process by which a mutant mtDNA molecule increases to high levels within a single cell containing both wild-type and ...
Han Jia-Jia - - 2012
Xylem development is a process of xylem cell terminal differentiation, which includes initial cell division, cell expansion, secondary cell wall formation and programmed cell death (PCD). PCD in plants and apoptosis in animals share many common characteristics. Caspase-3, which displays DEVD specificity, is a crucial executioner during animal cells apoptosis. ...
Rzayev Zakir M O - - 2012
A novel carboxyl-trithiocarbonate functionalized polymer with a highly selective antitumor activity was synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization of maleic anhydride (MA) with benzoyl peroxide as an initiator and S-1-dodecyl-S-(α,α'-dimethyl-α″-acetic acid)trithiocarbonate as a RAFT agent with the aim to design and synthesize an effective anticancer agent with ...
Bauer Kerry M KM Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, - - 2012
A label-free mass spectrometric strategy was used to examine the effect of 5-fluorouracil (5-FU) on the primary and metastatic colon carcinoma cell lines, SW480 and SW620, with and without treatment. 5-FU is the most common chemotherapeutic treatment for colon cancer. Pooled biological replicates were analyzed by nanoLC-MS/MS and protein quantification ...
Kořínek Marek - - 2013
We optimized and validated a rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of six metabolites of homocysteine metabolism: homocysteine, methionine, cysteine, S-adenosylmethionine, S-adenosylhomocysteine and betaine. The detection limits for these metabolites were in the nanomolar range, and the intra- and inter-day precisions were lower than ...
Chen Lei - - 2012
Cell wall proteins (CWPs) play a vital role in the development of the different morphological stages including mycelium, fruiting body and sclerotium in mushrooms which are important human food sources. Using fractionation by detergents and reducing agents, mushroom cell wall fractions from the different developmental stages of Pleurotus tuber-regium (PTR) ...
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