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Fahy Gregory M GM 21st Century Medicine, Inc., 14960 Hilton Drive, Fontana, CA, 92336, USA, - - 2015
Vitrification is an alternative approach to cryopreservation that enables hydrated living cells to be cooled to cryogenic temperatures in the absence of ice. Vitrification simplifies and frequently improves cryopreservation because it eliminates mechanical injury from ice, eliminates the need to find optimal cooling and warming rates, eliminates the importance of ...
Matsui Tomohiro T Department of Laboratory Sciences, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-kogushi, Ube, Yamaguchi, 755-8505, Japan, - - 2014
T cells infiltrate into the infarcted brain within days after cerebral ischemia and play essential roles in exacerbating the delayed phase of the brain injury by producing pro-inflammatory factors. However, the involvement of these factors in brain damage is also demonstrated systemically. Such periphery-brain abnormalities are interesting because they may ...
Baer Romain R UMR1037, Le Centre de Recherches en Cancérologie de Toulouse (CRCT), Inserm, F-31000 Toulouse, France; UMR1037, CRCT, Université Toulouse III-Paul Sabatier, F-31000 Toulouse, France; Equipe Labellisée Ligue Contre le Cancer, F-31000 Toulouse, - - 2014
Increased PI 3-kinase (PI3K) signaling in pancreatic ductal adenocarcinoma (PDAC) correlates with poor prognosis, but the role of class I PI3K isoforms during its induction remains unclear. Using genetically engineered mice and pharmacological isoform-selective inhibitors, we found that the p110α PI3K isoform is a major signaling enzyme for PDAC development ...
Li Ju J Department of Bioengineering, University of California-Berkeley, Berkeley, CA, 94720, USA; California Institute for Quantitative Biosciences (QB3), Berkeley, CA, 94720, - - 2014
The regenerative capacity of muscle dramatically decreases with age because old muscle stem cells fail to proliferate in response to tissue damage. Here we uncover key age-specific differences underlying this proliferative decline: namely, the genetic loci of CDK inhibitors (CDKI) p21 and p16 are more epigenetically silenced in young muscle ...
Ochieng John B JB a Department of Pediatrics , University of Virginia School of Medicine , Charlottesville , VA , - - 2014
Diarrhea causes substantial morbidity and mortality in children in low-income countries. Although numerous pathogens cause diarrhea, the etiology of many episodes remains unknown. Serratia marcescens is incriminated in hospital-associated infections, and HIV/AIDS associated diarrhea. We have recently found that Serratia spp. may be found more commonly in the stools of ...
Szabolcsi Viktória V Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg, Rte Albert Gockel 1, CH-1700, Fribourg, - - 2014
The calcium-binding protein parvalbumin (PV) hallmarks subpopulations of interneurons in the murine brain. We serendipitously observed the de novo expression of PV in ependymal cells of the lateral ventricle wall following in vivo lesioning and brain slicing for the preparation of organotypic hippocampal slice cultures (OHSCs). In OHSCs, de novo ...
Terzi Menderes Yusuf MY Neurosurgery Department and Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, Berlin, - - 2014
Purpose: Pigment epithelium-derived factor (PEDF) is a multifunctional protein with antiangiogenic, anti-inflammatory, neurotrophic and neurogenic properties. The effect of PEDF on traumatic brain injury (TBI) has not been explored. In this study, we aimed to show the in vivo effects of PEDF on lesion volume, cell death and cell proliferation ...
Herrgen Leah L Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, - - 2014
Both developing and adult organisms need efficient strategies for wound repair. In adult mammals, wounding triggers an inflammatory response that can exacerbate tissue injury and lead to scarring. In contrast, embryonic wounds heal quickly and with minimal inflammation, but how this is achieved remains incompletely understood. Using in vivo imaging in the ...
Romero Freddy F Thomas Jefferson University, Translational Medicine and Jane and Leonard Korman Lung Center, Philadelphia, Pennsylvania, United States ; - - 2014
Lipid-laden macrophages "foam cells" are observed in the lungs of patients with fibrotic lung disease but their contribution to disease pathogenesis remains unexplored. Here, we demonstrate that fibrosis induced by bleomycin, silica dust, or thoracic radiation promotes early and sustained accumulation of foam cells in the lung. In the bleomycin ...
Kuznetsov Andrey V AV Cardiac Surgery Research Laboratory, Department of Cardiac Surgery, Center of Operative Medicine, Innsbruck Medical University, Innsbruck A-6020, Austria. Electronic address: - - 2014
Dysfunction of cardiac energy metabolism plays a critical role in many cardiac diseases, including heart failure, myocardial infarction and ischemia-reperfusion injury and organ transplantation. The characteristics of these diseases can be elucidated in vivo, though animal-free in vitro experiments, with primary adult or neonatal cardiomyocytes, the rat ventricular H9c2 cell ...
Hindley Christopher J - - 2014
The liver is capable of full regeneration following several types and rounds of injury, ranging from hepatectomy to toxin-mediated damage. The source of this regenerative capacity has long been a hotly debated topic. The damage response that occurs when hepatocyte proliferation is impaired is thought to be mediated by oval/dedifferentiated ...
Tae Hyun-Jin HJ Laboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, Maryland ; Current affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, - - 2014
We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem histomorphologic analyses to have a practical reference for future clinical applications. Vessel ultrasound sonography was performed ...
Zhang Min M Division of Oral Pathology, Kyushu Dental University, 2-6-1 ManazuruKokurakita-ku, Kitakyushu, Fukuoka, 803-8580, - - 2014
Pulpotomy involves the removal of the coronal portion of pulp, including the diseased tissue, with the intent of maintaining the vitality of the remaining pulpal tissue via a therapeutic dressing. Once odontoblasts suffer injuries, the differentiation of mesenchymal cells is induced from the precursor cell population in the dental pulp, ...
Marudamuthu Amarnath Satheesh AS Department of Medicine, Texas Lung Injury Institute, University of Texas Health Science Center at Tyler, Tyler, - - 2014
Alveolar type II epithelial (ATII) cell injury precedes development of pulmonary fibrosis. Mice lacking urokinase-type plasminogen activator (uPA) are highly susceptible, whereas those deficient in plasminogen activator inhibitor (PAI-1) are resistant to lung injury and pulmonary fibrosis. Epithelial-mesenchymal transition (EMT) has been considered, at least in part, as a source ...
Zhou Min M 1Department of Respiratory Medicine, Kanazawa Medical University, Ishikawa, Japan. 2Research Institute of Respiratory Diseases, Department of Respiratory Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 3Department of Microbiology, Kanazawa Medical University, Ishikawa, - - 2014
Lysophosphatidylcholine is generated through the hydrolysis of phosphatidylcholine by phospholipase A2 and reversely converted to phosphatidylcholine by lysophosphatidylcholine acyltransferase 1. Although lysophosphatidylcholine is a potent proinflammatory mediator and increased in several types of acute lung injuries, the role of lysophosphatidylcholine acyltransferase 1 has not yet been addressed. We aimed to ...
Hong Sujin S 1 Iowa State University , Department of Food Science and Human Nutrition, Ames, - - 2014
Abstract This study evaluated the influence of starvation on the radiation resistance and injury in Escherichia coli O157:H7, following electron beam irradiation in 0.85% (wt/vol) saline and in apple juice. Washed exponential-phase cells of E. coli O157:H7 that were grown in tryptic soy broth (TSB) at 35°C were starved in ...
Tian Xing X Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang, 110840, - - 2014
Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of Arctium lappa L. roots (EAL) on hydrogen peroxide (H2O2)-induced cell injury in human SH-SY5Y ...
Nakagawa Shunsaku S Kyoto University Hospital - - 2014
Acute kidney injury (AKI) often correlates with poor prognosis, and is followed by various severe unfavorable systemic outcomes. It is important to understand the pathophysiology of AKI for the development of novel therapeutic approaches towards promoting renal regeneration after injury. Recent studies have indicated that AKI-induced tubular cell death plays ...
Adam Damien D INSERM UMR-S 903, SFR CAP-SANTE (FED 4231), University of Reims Champagne-Ardenne, 45 rue Cognacq-Jay, 51100, Reims, - - 2014
Chronic inflammation is a hallmark of Cystic Fibrosis (CF) lung disease and airway epithelium damage and remodelling are important components of lung pathology progression in CF. Whether this remodelling is secondary to deleterious infectious and inflammatory mediators, or to alterations of CF human airway epithelial (HAE) cells such as their ...
Caroleo Maria Cristina MC Department of Pharmacy, Nutrition and Health Sciences, University of Calabria , Cosenza , Italy - - 2014
Abstract Human podocytes are highly specialized cells with a key role in kidney physiology. Alteration of their structure as a consequence of injury or developmental failure leads to severe renal diseases. Although several studies have tried to elucidate the molecular framework of this cellular system, the functional bases for the ...
Thomasova Dana D Nephrologisches Zentrum, Medizinische Klinik und Poliklinik IV, Klinikum der Universität, Munich, Germany; - - 2014
Murine double minute-2 (MDM2), an E3 ligase that regulates the cell cycle and inflammation, is highly expressed in podocytes. In podocyte injury, MDM2 drives podocyte loss by mitotic catastrophe, but the function of MDM2 in resting podocytes has not been explored. Here, we investigated the effects of podocyte MDM2 deletion ...
Murtaugh L Charles LC Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112; email: - - 2014
Pancreatitis is caused by inflammatory injury to the exocrine pancreas, from which both humans and animal models appear to recover via regeneration of digestive enzyme-producing acinar cells. This regenerative process involves transient phases of inflammation, metaplasia, and redifferentiation, driven by cell-cell interactions between acinar cells, leukocytes, and resident fibroblasts. The ...
Li Guang G Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, 117597, - - 2014
The present study was designed to investigate whether H2S can protect testicular germ cells against heat exposure induced injury and the underlying mechanisms. It was found that all three H2S generating enzymes, cystathionine β-synthase (CBS), cystathionine γ-lysase (CSE), and 3-mercaptopyruvate sulfurtransferase (3MST), were expressed in mouse testicular tissue. Three episodes ...
Xie Fengshan F Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. - - 2014
Endothelial cell injury is an essential component of atherosclerosis and hypertension. Atherosclerosis and other macrovascular diseases are the most common complications of diabetes. Vaccarin is a major flavonoid glycoside in Vaccariae semen, and is expected to be useful in the treatment of vascular diseases. The aim of the present study ...
Arciniegas Enrique E Institute of Biomedicine "Dr. Jacinto Convit", and Service Autonomous Institute of Biomedicine, Central University of Venezuela, Caracas, Venezuela. - - 2014
Mucin 1 (MUC1) is a transmembrane glycoprotein that protects epithelial cells from injury caused by external stimuli. In addition to this role, MUC1 is involved in cell-cell adhesion, proliferation, motility, invasion and survival. In epithelial cells, MUC1 expression is regulated by binding of TNFα to TNFR1 and activation of the ...
Su Jingjing J Department of Neurology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200011, - - 2014
Cell cycle processes play a vital role in vascular endothelial proliferation and dysfunction. Cell division cycle protein 14 (Cdc14) is an important cell cycle regulatory phosphatase. Previous studies in budding yeast demonstrated that Cdc14 could trigger the inactivation of mitotic cyclin-dependent kinases (Cdks), which are required for mitotic exit and ...
Hovatta Outi O Departments of Clinical Science, Intervention and Technology and Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden; Karolinska University Hospital, Stockholm, Sweden; Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, Singapore - - 2014
Human embryonic stem cells have been considered the gold standard as a cell source for regenerative medicine since they were first cultured in 1998. They are pluripotent and can form principally all the cells types in the body. They are obtained from supernumerary human in vitro fertilization embryos that cannot ...
Iwakura Takamasa T Internal Medicine I, Division of Nephrology, Hamamatsu University School of Medicine, Hamamatsu, - - 2014
Proximal tubule (PT) cells can proliferate explosively after injurious stimuli. To investigate this proliferative capacity, we examined cell cycle status and the expression of cyclin-dependent kinase inhibitor p27, a G1 phase mediator, in PT cells after a proliferative or injurious stimulus. Rats were treated with lead acetate (proliferative stimulus) or ...
Yang Chun-Tao CT Department of Physiology, Guangzhou Medical University, Guangzhou, P.R. - - 2014
Background/Aim: Delayed wound healing is a common skin complication of diabetes, which is associated with keratinocyte injury and dysfunction. Levels of methylglyoxal (MGO), an α-dicarbonyl compound, are elevated in diabetic skin tissue and plasma, while levels of hydrogen sulfide (H2S), a critical gaseous signaling molecule, are reduced. Interestingly, the gas ...
Wu Mingchun M Department of Anesthesiology, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, - - 2014
Emerging evidences suggest that nicotine exerts a neuroprotective effect on Alzheimer's disease (AD), yet the precise mechanism is not fully elucidated. Here, HT22 cells were exposed to amyloid beta protein fragment (Aβ)1-42 to mimic the pathological process of neuron in AD. We hypothesized that cannabinoid receptor CB1 is involved in ...
Ong S-B - - 2014
The mechanism through which the protein kinase Akt (also called PKB), protects the heart against acute ischaemia-reperfusion injury (IRI) is not clear. Here, we investigate whether Akt mediates its cardioprotective effect by modulating mitochondrial morphology. Transfection of HL-1 cardiac cells with constitutively active Akt (caAkt) changed mitochondrial morphology as evidenced ...
Glowacki Andrew J AJ Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, - - 2014
Disease and injury perturb the balance of processes associated with inflammation and tissue remodeling, resulting in positive feedback loops, exacerbation of disease and compromised tissue repair. Conversely, under homeostatic healthy conditions, these processes are tightly regulated through the expansion and/or recruitment of specific cell populations, promoting a balanced steady-state. Better ...
Linck Guimarães Eduardo E Liver Cell Biology lab, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, 1090, - - 2014
Liver fibrosis is induced by the accumulation of extracellular matrix, deposited mainly by activated hepatic stellate cells (HSCs). One key characteristic of stellate cell activation is the directional migration to the site of injury during the wound-healing process. P311 is a protein that has been shown to play a role ...
MikuĊ‚a-Pietrasik Justyna J Laboratory of Gerontology, Department of Pathophysiology, These authors contributed equally to this - - 2014
There is agreement that the biological properties of additionally hydroxylated analogs of resveratrol (RVT) may be stronger compared with their parent drug. Here we examined the effect of a novel RVT derivative, 3,3',4,4'-tetrahydroxy-trans-stilbene (3,3',4,4'-THS), on the replicative senescence of human peritoneal mesothelial cells. The study showed that 3,3',4,4'-THS improves the ...
Qin Yu Y Cadet Brigade, Third Military Medical University, Chongqing, People's Republic of - - 2014
New neurons are continuously generated throughout life in the subgranular zone in the dentate gyrus of the mammalian hippocampus and in the subventricular zone of the lateral ventricles. With the aid of new methodologies, significant progress has been made in the characterization of endogenous stem cells (ependymal cells) and their ...
Brechbuhl Heather M HM National Jewish - - 2014
Rational: ERB family receptors (EGFR, ERB-B2, ERB-B3, and ERB-B4) regulate epithelial cell function in many tissue types. In the human airway epithelium, changes in ERB receptor expression are associated with epithelial repair defects. However, the specific role(s) played by ERB receptors in repair have not been determined. Objective: To determine ...
Zhao Jun J Department of Cancer Systems Imaging, The University of Texas MD Anderson Cancer Center, 1555 Holcombe Blvd., Houston, TX 77030, - - 2014
The treatment of liver injuries or hepatocellular carcinoma (HCC) has been hindered by the lack of efficient drug delivery. Even with the help of nanoparticles or other synthetic delivering agents, a large portion of the dose is still sequestered in the reticuloendothelial system. As an alternative, adipose-derived mesenchymal cells (AD-MSCs), ...
Higuita-Castro Natalia N Biomedical Engineering Department, The Ohio State University, Columbus, - - 2014
Interfacial flows during cyclic airway reopening are an important source of ventilator-induced lung injury. However, it is not known how changes in airway wall compliance influence cell injury during airway reopening. We used an in vitro model of airway reopening in a compliant microchannel to investigate how airway wall stiffness ...
Macho-Fernandez E E Trudeau Institute, Saranac Lake, New York, - - 2014
The immune mechanisms regulating epithelial cell repair after injury remain poorly defined. We demonstrate here that lymphotoxin beta receptor (LTβR) signaling in intestinal epithelial cells promotes self-repair after mucosal damage. Using a conditional gene-targeted approach, we demonstrate that LTβR signaling in intestinal epithelial cells is essential for epithelial interleukin-23 (IL-23) ...
Kramann Rafael R Brigham and Women's Hospital, Boston, MA, USA Harvard Medical School, Boston, MA, USA Division of Nephrology, RWTH Aachen University, Aachen, - - 2014
The kidney possesses profound regenerative potential and in some cases can recover completely 'restitutio at integrum' following an acute kidney injury (AKI). Emerging evidence strongly suggests that sometimes repair is incomplete, however, and, in this situation, an episode of AKI leads to future chronic kidney disease (CKD). Understanding the tubular ...
Liu Peng P Department of Hepatobiliary Surgery, The Navy General Hospital, Beijing, 100048, People's Republic of - - 2014
To investigate the effects of icaritin, an active ingredient extracted from Epimedium Sagittatum (Sieb. et Zucc.), on CCl4-induced liver injury and its possible mechanisms. Hepatocytes isolated from Sprague-Dawley male rats were treated with 3 mmol/L CCl4 for 24 h to induce acute liver cell injury, then icaritin (0.1, 1, 10, 100 μmol/L, respectively) ...
Liu Jo-Wen JW Center For Health Disparities and Molecular Medicine, Loma Linda University School of Medicine, Loma Linda, CA, - - 2014
Epidermal-fatty acid binding protein (E-FABP/ FABP5/DA11) binds and transport long-chain fatty acids in the cytoplasm and may play a protecting role during neuronal injury. We examined whether E-FABP protects nerve growth factor differentiated PC12 cells (NGFDPC12 cells) from lipotoxic injury observed after palmitic acid (C16:0; PAM) overload. NGFDPC12 cells cultures ...
Masterson Claire - - 2014
Rojas and colleagues have presented an exciting paper demonstrating yet another relevant preclinical setting in which the mesenchymal stromal cell has a potential therapeutic application. What is particularly interesting about this study is that it addresses a disease, blood-borne systemic sepsis, which has multiple complex host responses and involves a ...
Berger Katja K Division of Nephrology and Clinical Immunology, Rheinisch-Westfaelische Technische Hochschule (RWTH), Aachen, - - 2014
Acute kidney injury (AKI) is a common clinical problem and is associated with high mortality rates. It is accepted that after AKI cellular regeneration of the proximal tubule occurs from intrinsic tubule cells. Recently, scattered tubular cells (STCs) were discovered as a novel subpopulation of tubule cells involved in regeneration. ...
Kurian Gino A GA School of Chemical and Biotechnology, SASTRA University, Thanjavur-613 401, - - 2014
Renal ischemia reperfusion injury contributes patho-physiological imbalance of acute renal failure that comprises of generation of reactive oxygen species, nitric oxide and peroxynitrite and inflammation involving cytokine/adhesion molecule cascade, finally leads to cell death. Oxygen deprival associated with ischemia that in turn lead to decline ATP production is the characteristic ...
Chen C Russell CR Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, Queen Square, London, United - - 2014
Studies using animal models have recently suggested that the olfactory mucosa may be a source of cells capable of stimulating and contributing to complex neurologic regeneration. Several groups have already transplanted cell derivatives from the olfactory mucosa into injury models, and the results so far have been promising. To fully ...
Mohammadi Tamimount T Membrane Biochemistry and Biophysics, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht (The - - 2014
Getting visible: A new method to label bacterial cell walls shows the presence of functional peptidoglycan in the important pathogen Chlamydia trachomatis. This might clarify the long-standing paradox of the "chlamydial anomaly".
Yamazaki Tomohiro T Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan. Electronic address: - - 2014
Chlamydia trachomatis is an obligate intracellular bacterium that causes a sexually transmitted disease. Ureaplasma parvum is commensal in the human genital tract, with a minimal contribution to urogenital infection. We have recently found that U. parvum has a significant effect on the presence of C. trachomatis in the genital tract of healthy ...
Abdanipour Alireza A Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital, Tehran, - - 2014
To evaluate the effects of glial cell line-derived neurotrophic factor transplantation combined with adipose-derived stem cells-transdifferentiated motoneuron delivery on spinal cord contusion injury, we developed rat models of spinal cord contusion injury, 7 days later, injected adipose-derived stem cells-transdifferentiated motoneurons into the epicenter, rostral and caudal regions of the impact ...
Frandi Antonio A Department of Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine/CMU, University of Geneva, Rue Michel Servet 1, 1211 Genève 4, - - 2014
The nature and assembly of the chlamydial division septum is poorly defined due to the paucity of a detectable peptidoglycan (PG)-based cell wall, the inhibition of constriction by penicillin and the presence of coding sequences for cell wall precursor and remodelling enzymes in the reduced chlamydial (pan-)genome. Here we show ...
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