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Mandalari Giuseppina G The Model Gut, Institute of Food Research,NorwichNR4 - - 2014
A number of studies have demonstrated that consuming almonds increases satiety but does not result in weight gain, despite their high energy and lipid content. To understand the mechanism of almond digestion, in the present study, we investigated the bioaccessibility of lipids from masticated almonds during in vitro simulated human ...
Divyashri Gangaraju G Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110 025, - - 2014
Gamma-aminobutyric acid (GABA) production by free and Ca-alginate encapsulated cells of Enterococcus faecium CFR 3003 was investigated. Mass transfer rates characterizing the GABA production process using encapsulated cells were investigated. Experiments were performed to investigate external film and internal pore diffusion mass transfer rates. The Damkohler and Thiele analysis provides ...
Cen Jialiang - - 2014
Highly-scaled direct gravure is a promising printing technique for printed electronics due to its large throughput, high resolution and simplicity. Gravure can print features in the single micron range at printing speeds of ~1 m/s by using an optimized cell geometry and optimized printing conditions. The filling of the cells ...
Eagling Tristan - - 2014
Iron bioavailability in unleavened white and wholegrain bread made from two commercial wheat varieties was assessed by measuring ferritin production in Caco-2 cells. The breads were subjected to simulated gastrointestinal digestion and the digests applied to the Caco-2 cells. Although Riband grain contained a lower iron concentration than Rialto, iron ...
Rodrigues Francisca F Department of Chemical Sciences, Faculty of Pharmacy, REQUIMTE - - 2014
Abstract Objective: The aim of this study was to develop and characterize suppositories for vaginal delivery of Lactobacillus acidophilus. Methods: Formulations were performed in order to select suitable excipients based on suppository formation feasibility and cytotoxicity. Solid body and hollow-type suppositories were prepared by melting and molding using poly(ethylene glycol) ...
Liao Wen-Pin - - 2014
The finite difference time domain simulation shows the existence of an asymmetric quadrupole of Fano resonance on the surface of gold-silica core-shell (Au@silica) nanoparticle (NP) as being incorporated into the metal oxide nanoarchitecture/P3HT hybrid. Compared to the metal oxide nanoarchitecture/P3HT hybrid solar cell, a 30% enrichment of the short-circuit current ...
Richter V V Institute of Applied Research, Aalen University, Beethovenstr. 1, 73430, Aalen, - - 2014
An inverted microscope has been modified for light scattering experiments with high angular resolution in combination with transmission, wide-field fluorescence or laser scanning microscopy. Supported by simulations of Mie scattering, this method permits detection of morphological changes of 3T3 fibroblasts on apoptosis and formation of spherically shaped cells of about ...
Katzengold Rona R Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, 69978, Tel Aviv, - - 2014
This study introduces new three-dimensional finite element cell modeling for simulating the structural, large deformation behavior of maturing adipocytes, based on empirically acquired geometrical properties of cultured adipocyte cells. We created models of adipocyte differentiation and maturation, which represented four stages along that process. The modeling focused on two specific ...
Kamal Ahmed A Medicinal Chemistry and Pharmacology, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India. - - 2014
A number of pyrazole-oxadiazole conjugates were synthesized and evaluated for their ability to function as antiproliferative agents on various human cancer cell lines. These conjugates are comprised of pyrazole and oxadiazole scaffolds closely attached to each other without any spacer as two structural classes. The Type I class has a ...
Fackler Oliver T OT 1] Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany. [2] German Center for Infection Research, Heidelberg University, 69120 Heidelberg, - - 2014
In vitro studies in primary or immortalized cells continue to be used to elucidate the essential principles that govern the interactions between HIV-1 and isolated target cells. However, until recently, substantial technical barriers prevented this information from being efficiently translated to the more complex scenario of HIV-1 spread in the ...
Wang Sheng-Fan SF Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan AIDS Prevention and Research Center, National Yang-Ming University, Taipei, - - 2014
Galectin-3 has been reported to regulate the functions of a number of immune cell types. We previously reported that galectin-3 is translocated to immunological synapses in T cells upon T cell receptor engagement, where it associates with ALG-2-interacting protein X (Alix). Alix is known to coordinate with the endosomal sorting ...
Mantri C K CK Department of Oral Biology, School of Dentistry, Meharry Medical College, Nashville, TN, - - 2014
HIV-1 relies on the host's cell machinery to establish a successful infection. Surface receptors, such as CD4, CCR5, and CXCR4 of T cells and macrophages, are essential for membrane fusion of HIV-1, an initiate step in viral entry. However, it is not well defined how HIV-1 infects CD4-negative mucosal epithelial ...
Barreto-de-Souza Victor V Section of Intercellular Interactions, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, - - 2014
The vast majority of new HIV infections in male-to-female transmission occurs through semen, where HIV-1 is present in two different forms: as free and as cell-associated virus. In the female lower genital tract, semen mixes with female genital secretions that contain various factors, some of which facilitate or inhibit HIV-1 ...
Badia Roger R IrsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, 08916 Badalona, - - 2014
To characterize a new zinc-finger nuclease (ZFN) that targets close to the sequence of the 32 bp deletion polymorphism in the CCR5 gene, and to generate cells resistant to HIV-1 strains that use CCR5. CCR5Δ32 is a naturally occurring deletion that provides genetic resistance to R5-tropic HIV-1. The specificity and ...
Reinhard Christian - - 2014
SIVMAC/HIV-2 Vpx recruits the CUL4A-DCAF1 E3 ubiquitin ligase complex to degrade the deoxynucleotide hydrolase SAMHD1. This increases the concentration of deoxynucleotides available for reverse transcription in myeloid cells and resting T cells. Accordingly, transduction of these cells by SIVMAC requires Vpx. Virus-like particles containing SIVMAC Vpx (Vpx-VLPs) also increase the ...
De Iaco Alberto - - 2014
The human peptidyl-prolyl isomerase Cyclophilin A (CypA) binds HIV-1 capsid (CA) and influences early steps in the HIV-1 replication cycle. The mechanism by which CypA regulates HIV-1 transduction efficiency is unknown. Disruption of CypA binding to CA, either by genetic means or by the competitive inhibitor cyclosporine A (CsA), reduces ...
Liu Jun J Clinical Sciences Division, University of Toronto, Toronto, Ontario, - - 2014
A rare subset of IL-10-producing B cells, named regulatory B cells (Bregs), suppresses adaptive immune responses and inflammation in mice. In this study, we examined the role of IL-10-producing B cells in HIV-1 infection. Compared to uninfected controls, IL-10-producing B cell frequencies were elevated in both blood and sigmoid colon ...
Kinlock Ballington L BL Center for AIDS Health Disparities Research, Meharry Medical College, Nashville, Tennessee, United States of America; Department of Microbiology and Immunology, Meharry Medical College, Nashville, Tennessee, United States of - - 2014
While it is accepted that viruses can enter epithelial cells by endocytosis, the lack of an established biological mechanism for the trafficking of infectious virions through vaginal epithelial cells and their release from the plasma membrane has contributed to ongoing controversy about whether endocytosis is a mere artifact of some ...
Kohli Arinder A Department of Oral Immunology, Clinical and Diagnostic Sciences, King's College London Dental Institute, King's College London, London, United - - 2014
The majority of HIV-1 infections worldwide are acquired via mucosal surfaces. However, unlike the vaginal mucosa, the issue of whether the oral mucosa can act as a portal of entry for HIV-1 infection remains controversial. To address potential differences with regard to the fate of HIV-1 after exposure to oral ...
Martinsen F A FA Department of Physics, Norwegian University of Science and Technology, N-7491, Trondheim, - - 2014
Vertically aligned radial-junction solar cell designs offer potential improvements over planar geometries, as carrier generation occurs close to the junction for all absorption depths, but most production methods still require a single crystal substrate. Here, we report on the fabrication of such solar cells from polycrystalline, low purity (99.98%) p-type ...
Ishida Sumire S Transdisciplinary Life Science Course, Faculty of Advanced Life Science, Hokkaido University, Sapporo, - - 2014
Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen ...
Masiello Maria Grazia MG Department of Clinical and Molecular Medicine, "Sapienza" University of Rome, Piazza Sassari 3, 00161 Rome, Italy ; Department of Surgery "PietroValdoni", "Sapienza" University of Rome, Via A. Scarpa 14, 00161 Rome, - - 2014
Microgravity exerts dramatic effects on cell morphology and functions, by disrupting cytoskeleton and adhesion structures, as well as by interfering with biochemical pathways and gene expression. Impairment of cells behavior has both practical and theoretical significance, given that investigations of mechanisms involved in microgravity-mediated effects may shed light on how ...
Ma Jingtao J School of Life Science, Beijing Institute of Technology, Beijing 100081, - - 2014
Separation of two categories of cells in pinched flow fractionation(PFF) device is simulated by employing IB-LBM. The separation performances at low Reynolds number (about 1) under different pinched segment widths, flow ratios, cell features, and distances between neighboring cells are studied and the results are compared with those predicted by ...
Mehrali Mehdi M Department of Mechanical Engineering and Center of advanced Material, University of Malaya, Kuala Lumpur, - - 2014
Calcium silicate (CaSiO3, CS) ceramic composites reinforced with graphene nanoplatelets (GNP) were prepared using hot isostatic pressing (HIP) at 1150°C. Quantitative microstructural analysis suggests that GNP play a role in grain size and is responsible for the improved densification. Raman spectroscopy and scanning electron microscopy showed that GNP survived the ...
Dhakal Kamal K Biophysics and Physiology Lab, Department of Physics, University of Texas at Arlington, Texas, - - 2014
The selective insertion of foreign materials, such as fluorescent markers or plasmids, into living cells has been a challenging problem in cell biology due to the cell membrane's selective permeability. However, it is often necessary that researchers insert such materials into cells for various dynamical and/or drug delivery studies. This ...
Han Xin - - 2013
Herein, we report the development of efficient inhibitors of reverse transcriptase (RT) of HIV-1 based on indole-alkyl trifluoropyruvate derivatives by a TZM-bl cell assay. The inhibitory activities of the two enantiomers and the corresponding racemic mixture have been compared. TZM-bl cells exhibited strong enantioselective discrimination for the (R)-configuration, among these ...
Wei Bin - - 2013
Immune cell adaptor protein ADAP (adhesion and degranulation-promoting adaptor protein) mediates aspects of T-cell adhesion and proliferation. Despite this, a connection between ADAP and infection by the HIV-1 (human immunodeficiency virus-1) has not been explored. In this paper, we show for the first time that ADAP and its binding to ...
Hatch Steven C SC Viral Recombination - - 2013
The enrichment of HIV-1 macromolecules at the uropod of polarized T cells can significantly promote virus assembly and cell-mediated infection. Using live-cell fluorescence microscopy, we demonstrate that full-length HIV-1 RNA is enriched at the uropod membrane; furthermore, the presence of HIV-1 Gag containing a functional nucleocapsid domain is necessary for ...
Markle Tristan J - - 2013
HIV-1 Nef is a multifunctional viral protein that contributes to higher plasma viremia and more rapid disease progression. Nef appears to accomplish this, in part, through modulation of T-cell activation; however, the results of these studies over the past 25 years have been inconsistent. Here, the history of contradictory observations ...
Ding Donglin - - 2013
Understanding the mechanism of HIV-1 latency is crucial to eradication of the viral reservoir in HIV-1-infected individuals. However, the role of histone methyltransferase (HMT) G9a-like protein (GLP) in HIV-1 latency is still unclear. In the present work, we established four clonal cell lines containing HIV-1 vector. We found that the ...
Anand Appakkudal R - - 2013
ABSTRACT: BACKGROUND: Slit2 is a ~ 200 kDa secreted glycoprotein that has been recently shown to regulate immune functions. However, not much is known about its role in HIV (human immunodeficiency virus)-1 pathogenesis. RESULTS: In the present study, we have shown that the N-terminal fragment of Slit2 (Slit2N) (~120 kDa) ...
Tada Takuya T Center for Experimental Medicine and System Biology, Institute of Medical Science, University of Tokyo Tokyo, Japan ; Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo Tokyo, Japan ; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Saitama, Japan ; Department of Pathology, National Institute of Infectious Diseases Tokyo, - - 2013
Attempts to create mouse models for AIDS have been hampered by species barriers in HIV-1 infection. We previously showed that the nuclear accumulation of HIV-1 preintegration complex (PIC) was suppressed in mouse cells. Lens epithelium-derived growth factor (LEDGF/p75) is a host factor identified as a binding partner of integrase (IN), ...
Veillette Maxime M Department of Medical Biology, Laboratory of retrovirology and GROEM, Université du Québec à Trois-Rivières, 3351 Boulevard des Forges, CP500, Trois-Rivières, QC G9A 5H7, - - 2013
HIV-1 is inhibited early after entry into cells expressing some simian orthologues of the tripartite motif protein family member TRIM5α. Mutants of the human orthologue (TRIM5αhu) can also provide protection against HIV-1. The host protein cyclophilin A (CypA) binds incoming HIV-1 capsid (CA) proteins and enhances early stages of HIV-1 ...
Liang Zhipin - - 2012
The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited the vpu gene expression; the other one over-expressed ...
Junior Celso O R - - 2012
Twenty analogues of the anti-HIV-1 integrase (IN) inhibitors dicaffeoylquinic acids (DCQAs) were prepared. Their IC(50) values for 3'-end processing and strand transfer against recombinant HIV-1IN were determined in vitro, and their cell toxicities and EC(50) against HIV-1 were measured in cells (ex vivo). Acetylated or benzylated and/or with cyclohexylidene group ...
Gupta Phalguni P Department of Infectious Diseases and Microbiology, School of Public Health, University of Pittsburgh , Pittsburgh, Pennsylvania 15261, USA. - - 2013
Microbicides have been evaluated mostly against cell-free HIV-1. Because semen contains both cell-free and cell-associated HIV-1, HIV-1 transmission could occur via either or both sources. Therefore, it is important to examine the antiviral activity of microbicides against cell-associated HIV-1. The cyclic antimicrobial peptide retrocyclin RC-101 has been shown previously to ...
Gianella Sara S University of California San Diego, La Jolla, CA 92093-0679, USA. - - 2012
We present a case of sexual transmission of HIV-1 predicted to have CXCR4-tropism during male-to-male sexual exposure. Phylogenetic analyses exclude cell-free virus in the seminal plasma of the source partner and possibly point to the seminal cells as the origin of the transmission event.
Schrader Christoph - - 2012
Lapatinib targets human epidermal growth factor-receptor (EGFR) and Her2/neu receptor tyrosine-kinases and hence is under investigation in multimodal therapy concepts of advanced head and neck squamous cell carcinoma (HNSCC). We studied combined effects of lapatinib and cisplatin on colony formation (CF) of epithelial cells of individual HNSCC in short term ...
Escamilla Juanita - - 2012
Probiotics have been shown to have a preventative role in colorectal carcinogenesis but research concerning their prophylactic potential in the later stages of colorectal cancer, specifically metastasis is limited. This study explored the potential of cell-free supernatants (CFS) from 2 probiotic Lactobacillus sp., Lactobacillus casei and Lactobacillus rhamnosus GG, to ...
Murfuni Ivana - - 2012
The Werner syndrome protein (WRN) is a member of the RecQ helicase family. Loss of WRN results in a human disease, the Werner syndrome (WS), characterised by high genomic instability, elevated cancer risk and premature aging. WRN is crucial for the recovery of stalled replication forks and possesses both helicase ...
Brown Guy C - - 2012
Phagoptosis, also called primary phagocytosis, is a recently recognised form of cell death caused by phagocytosis of viable cells, resulting in their destruction. It is provoked by exposure of 'eat-me' signals and/or loss of 'don't-eat-me' signals by viable cells, causing their phagocytosis by phagocytes. Phagoptosis mediates turnover of erythrocytes, neutrophils ...
Kim Young-Min - - 2012
Capsosiphon fulvescens is a well-known green sea algae that has been touted in recent years as a potential anticancer drug. In this study, C. fulvescens glycoprotein (Cf-GP) showed proapoptotic signaling in AGS cells. An MTS assay indicated that Cf-GP inhibited the proliferation of AGS cell lines in a dose-dependent manner. ...
Trinh Nguyen Thu Ngan - - 2012
Airway damage and remodelling are important components of lung pathology progression in cystic fibrosis (CF). Although repair mechanisms are engaged to restore the epithelial integrity, these processes are obviously insufficient to maintain lung function in CF airways. Our aims were therefore to study how the basic CFTR defect could impact ...
Bompard G G Universités Montpellier 2 et 1, Montpellier, - - 2013
The oncogenic kinase PAK4 was recently found to be involved in the regulation of the G1 phase and the G2/M transition of the cell cycle. We have also identified that PAK4 regulates Ran GTPase activity during mitosis. Here, we show that after entering mitosis, PAK4-depleted cells maintain a prolonged metaphase-like ...
Byrns Michael C MC Center of Excellence in Environmental Toxicology, Department of Pharmacology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United - - 2012
Type 5 17β-hydroxysteroid dehydrogenase (AKR1C3) is the major enzyme in the prostate that reduces 4-androstene-3,17-dione (Δ(4)-Adione) to the androgen receptor (AR) ligand testosterone. AKR1C3 is upregulated in prostate cancer (PCa) and castrate resistant prostate cancer (CRPC) that develops after androgen deprivation therapy. PCa and CRPC often depend on intratumoral androgen ...
Paracchini Valentina - - 2012
Cystic fibrosis (CF) is a monogenic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, with lung and liver manifestations. Because of pitfalls of gene therapy, novel approaches for reconstitution of the airway epithelium and CFTR expression should be explored. In the present study, human amniotic mesenchymal ...
Côté Marceline - - 2012
Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus that was originally identified from human prostate cancer patients and subsequently linked to chronic fatigue syndrome. Recent studies showed that XMRV is a recombinant mouse retrovirus; hence, its association with human diseases has become questionable. Here, we demonstrated that XMRV envelope ...
Ndirangu James J Africa Centre for Health and Population Studies, University of KwaZulu-Natal, Somkhele, South Africa. - - 2012
Transmission through breastfeeding remains important for mother-to-child transmission (MTCT) in resource-limited settings. We quantify the relationship between cell-free (RNA) and cell-associated (DNA) shedding of HIV-1 virus in breastmilk and the risk of postnatal HIV-1 transmission in the first 6 months postpartum. Thirty-six HIV-positive mothers who transmitted HIV-1 by breastfeeding were ...
Schrijvers Rik R Division of Molecular Medicine, Katholieke Universiteit Leuven, Leuven, Flanders, - - 2012
Lens epithelium-derived growth factor (LEDGF/p75) is a cellular co-factor of HIV-1 integrase (IN) that tethers the viral pre-integration complex to the host cell chromatin and determines the genome wide integration site distribution pattern of HIV-1. Recently, we demonstrated that HIV-1 replication was reduced in LEDGF/p75 knockout (KO) cells. LEDGF/p75 KO ...
Takeuchi Ichiro - - 2011
BACKGROUND: We previously reported that the level of high mobility group protein AT-hook 1 (HMGA1) is low in androgen-dependent prostate cancer (PCa) cells (LNCaP), but is high in androgen-independent PCa cells (DU145 and PC-3) and that HMGA1 is a strong candidate gene playing a potential role in the progression of ...
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