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ImbrasaitÄ—, A.
The aim of this study was to determine the factors responsible for the survival of 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-treated differentiating HL60 cells. After TPA treatment, HL60 cells in nonadherent cell fraction undergo apoptosis, whereas they survive and differentiate when attached to substrate. We have found that the amount of viable TPA-treated cells ...
Wang Zhenhong Z Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; College of Chemistry and Environment, Minnan Normal University, Zhangzhou 363000, - - 2014
Strategies used by Microcystis aeruginosa, bloom-forming cyanobacteria, for potential inorganic arsenic (arsenate and arsenite) uptake, and depuration kinetics under phosphate-enriched (+P) and depleted (-P) treatments were examined via short- and long-term experiments. Phosphate depletion improved arsenate or arsenite uptake rate constants. M. aeruginosa arsenite influx occurred considerably faster than arsenate ...
Ono Kaori K Graduate School of Life and Environmental Sciences, University of - - 2014
Pseudomonas aeruginosa responds to environmental changes and regulates its life cycle from planktonic to biofilm modes of growth. The control of cell attachment to surfaces is one of the critical processes that determine this transition.Environmental signals are typically relayed to the cytoplasm by second messenger systems. We here demonstrated that ...
Murphy Kathleen K Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, - - 2014
Pseudomonas aeruginosa is a common opportunistic human pathogen known for its ability to adapt to changes in its environment during the course of infection. These adaptations include changes in the expression of cell surface lipopolysaccharide (LPS), biofilm development, and the production of a protective extracellular exopolysaccharide matrix. Outer membrane vesicles ...
Ahire Jayesh J JJ Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South - - 2014
Pseudomonas aeruginosa forms biofilms in wounds, which often leads to chronic infections that are difficult to treat with antibiotics. Free iron enhances biofilm formation, delays wound healing, and may even be responsible for persistent inflammation, increased connective tissue destruction, and lipid peroxidation. Exposure of P. aeruginosa Xen 5 to the ...
Cabeen Matthew T MT Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of - - 2014
Secreted virulence factors of the human pathogen Pseudomonas aeruginosa are often under quorum sensing control. Cells lacking the quorum-sensing regulator LasR show reduced virulence factor production under typical laboratory conditions and are hypo-virulent in short-term animal infection models, yet lasR mutants are frequently associated with long-term infection in cystic fibrosis ...
Chew Su Chuen - - 2014
Biofilms are densely populated communities of microbial cells protected and held together by a matrix of extracellular polymeric substances. The structure and rheological properties of the matrix at the microscale influence the retention and transport of molecules and cells in the biofilm, thereby dictating population and community behavior. Despite its ...
Shao Jihai - - 2013
Sanguinarine showed strong inhibitory effect against Microcystis aeruginosa, a typical water bloom-forming and microcystins-producing cyanobacterium. The EC50 of sanguinarine against the growth of M. aeruginosa NIES-843 was 34.54=1.17μg/L. Results of chlorophyll fluorescence transient analysis indicated that all the electron donating side, accepting side, and the reaction center of the Photosystem ...
Purcell Diane D Environmental Science and Technology Department, Cranfield University, Cranfield, - - 2013
We report on the effectiveness of sonication on controlling the growth of four problematic algal species which are morphologically different and from three algal divisions. Two cyanobacterial species Microcystis aeruginosa (unicellular) and Aphanizomenon flos-aquae (filamentous), one green alga Scenedesmus subspicatus (colonial) and lastly a diatom species Melosira sp. (filamentous) were ...
Liedtke Carole M - - 2013
Focus on "Effect of apical hyperosmotic sodium challenge and amiloride on sodium transport in human bronchial epithelial cells from cystic fibrosis donors."
Rymut Sharon M SM Dept. of Pediatrics, Case Western Reserve Univ., 833 BRB, 10900 Euclid Ave., Cleveland, OH 44106-4948. - - 2013
Dysfunctional cystic fibrosis transmembrane conductance regulator (CFTR) leads to many cellular consequences, including perinuclear accumulation of free cholesterol due to impaired endosomal transport. The hypothesis being tested is that CF-related perinuclear cholesterol accumulation due to disrupted endocytic trafficking occurs as a result of reduced microtubule (MT) acetylation. Here, it is ...
Zhao Kun K Department of Bioengineering, University of California, Los Angeles, California 90095, - - 2013
Bacterial biofilms are surface-associated, multicellular, morphologically complex microbial communities. Biofilm-forming bacteria such as the opportunistic pathogen Pseudomonas aeruginosa are phenotypically distinct from their free-swimming, planktonic counterparts. Much work has focused on factors affecting surface adhesion, and it is known that P. aeruginosa secretes the Psl exopolysaccharide, which promotes surface attachment ...
Christova Neli - - 2013
A newly isolated indigenous strain BN10 identified as Pseudomonas aeruginosa was found to produce glycolipid (i.e., rhamnolipid-type) biosurfactants. Two representative rhamnolipidic fractions, RL-1 and RL-2, were separated on silica gel columns and their chemical structure was elucidated by a combination of nuclear magnetic resonance and mass spectroscopy. Subsequently, their cytotoxic ...
Pinzon Neissa M - - 2013
Rhamnolipids are high-value effective biosurfactants produced by Pseudomonas aeruginosa. Large scale production of rhamnolipids is still challenging especially under free-cell aerobic conditions in which the highly foaming nature of the culture broth reduces the productivity of the process. Immobilized systems relying on oxygen as electron acceptor have been previously investigated ...
Sanchez Zoe - - 2013
Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells.
Sodagari Maysam - - 2012
Bacterial attachment on solid surfaces has various implications in environmental, industrial and medical applications. In this study, the effects of rhamnolipid biosurfactants on initial attachment of bacteria on hydrophilic glass and hydrophobic octadecyltrichlorosilane (OTS) modified glass were evaluated under continuous-flow conditions. The bacteria investigated were three Gram-negative species Pseudomonas aeruginosa, ...
Tsybulin Olexandr - - 2012
A wide range of non thermal biological effects of microwave radiation (MW) was revealed during the last decades. A number of reports showed evident hazardous effects of MW on embryo development in chicken. In this study, we aimed at elucidating the effects of MW emitted by a commercial model of ...
Shao Jingwei - - 2012
Photodynamic therapy (PDT) has been accepted as an alternative treatment for cancer. The rationale for the development of PDT for cancer is that target specificity can be achieved by controlling the location at which light activates the drug, i.e. photosensitiser. Metal phthalocyanines represent a new class of photosensitisers developed for ...
Ozeki Ayumi - - 2012
Neural stem cells (NSCs) are highly susceptible to DNA double-strand breaks; however, little is known about the effects of radiation in cells surviving radiation. Although the nestin-positive NSCs predominantly became glial fibrillary acidic protein (GFAP)-positive in differentiation-permissive medium, little or no cells were GFAP positive in proliferation-permissive medium. We found ...
Yen Hong-Wei - - 2012
The results of this study indicate that the irradiation could enhance the cells growing of Rhodotorula glutinis to 54.2±1.6g/L as compared to the control (without irradiation) of 38.3±1.2g/L. However, different wavelength of LEDs' (red, green, blue and white) had no significant impacts on the growth and on the lipid content. ...
Cong Min - - 2012
Liver fibrosis is the result of the entire organism responding to a chronic injury. Every cell type in the liver contributes to the fibrosis. This paper first discusses key intracellular signaling pathways that are induced during liver fibrosis. The paper then examines the effects of these signaling pathways on the ...
Buch Karl - - 2012
For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be ...
Pan Jiachuan - - 2012
Bacteria are well known to form dormant persister cells that are tolerant to most antibiotics. Such intrinsic tolerance also facilitates the development of multidrug resistance through acquired mechanisms. Thus persister cells are a promising target for developing more effective methods to control chronic infections and help prevent the development of ...
Ou Huase - - 2011
In this study, the immediate and long-term impacts of shortwave ultraviolet (UV-C) irradiation on photosynthetic capacity, survival, and recovery of Microcystis aeruginosa were investigated. The risk of microcystin-LR (MC-LR) release during irradiation was also estimated. The cell density was determined by a flow cytometry, and typical chlorophyll fluorescence parameters, including ...
Garcia-Käufer Manuel - - 2011
PURPOSE: Ciprofloxacin (CIP), a broad-spectrum, second-generation fluoroquinolone, has frequently been found in hospital wastewaters and effluents of sewage treatment plants. CIP is scarcely biodegradable, has toxic effects on microorganisms and is photosensitive. The aim of this study was to assess the genotoxic potential of CIP in human HepG2 liver cells ...
Ye Yintao - - 2011
The aim of this study was to investigate the effect of dihydrotanshinone I (DI) in inhibiting the growth of human cervical cancer cells both in vitro and in vivo, and molecular targets in HeLa cells when treated by DI or irradiation with or without being combined. In this study, MTT, ...
Ng C K M - - 2011
We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on ...
Detty Michael R - - 2011
This article is a highlight of the paper by Anquez et al. in this issue of Photochemistry and Photobiology and describes the potential benefits of direct excitation of molecular oxygen to produce singlet oxygen rather than using a photosensitizer. Due to its simplicity, the direct excitation of molecular oxygen can ...
Nishiura Hideki - - 2011
We successfully identified the bystander effect in B16 murine melanoma cells exposed to UVA irradiation. The effect was identified based on melanogenesis following the medium transfer of the B16 cells, which had been cultured for 24 hours after being exposed to UVA irradiation, to non-irradiated cells (bystander cells). Our confirmation ...
Terai Takuya - - 2011
Biotin-(strept)avidin complex is widely used in biotechnology because of its extremely high binding constant, but there is no report describing spatiotemporally controlled formation of the complex in live cells. Here, based on X-ray crystal structure analysis and calorimetric data, we designed and synthesized photoreleasable biotins, which show greatly reduced affinity ...
Cho Min - - 2011
Motivated by recent studies that documented changes in fullerene toxicity after chemical transformation, C60 aggregates (nC60) were subject to UVC irradiation at monochromatic 254 nm and subsequently evaluated for antibacterial and bactericidal properties against Escherichia coli. The nC60 treated with UVC irradiation, referred to herein as UVC-irradiated C60, did not ...
Yang H - - 2011
In space, astronauts are exposed to radiation fields consisting of energetic protons and high atomic number, high-energy (HZE) particles at very low dose rates or fluences. Under these conditions, it is likely that, in addition to cells in an astronaut's body being traversed by ionizing radiation particles, unirradiated cells can ...
Allers Thorsten - - 2011
Type IV pili are filamentous structures that are found on the surface of many bacterial and archaeal cells, they are involved in cell motility and surface adhesion. In the crenarchaeon Sulfolobus solfataricus, type IV pili formation is strongly induced by UV irradiation and leads to cellular aggregation. The study by ...
Grassi F R - - 2011
Applications of laser therapy in biostimulation and healing injured tissues are widely described in medical literature. The present study focuses on the effects of laser irradiation on the growth rate and differentiation of human osteoblast-like cells seeded on titanium or zirconia surfaces. Cells were laser irradiated with low therapeutical doses ...
Canazza Alessandra - - 2011
A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8Gy was delivered intermittently over a period of 40 minutes. The ability of glioma cells to migrate into a matrigel matrix was evaluated on ...
Dickey Jennifer S - - 2011
This review focuses on a number of recent studies that have examined changes in microRNA (miRNA) expression profiles in response to ionizing radiation and other forms of oxidative stress. In both murine and human cells and tissues, a number of miRNAs display significant alterations in expression levels in response to ...
Sawajiri Masahiko - - 2011
To examine the effects of carbon ion and gamma ray irradiation on cancer-induced osteoclastogenesis, mouse calvaria MC3T3-E1 cells were cultured with conditioned medium from irradiated and non-irradiated MCF7 human breast cancer cells. The authors examined RANKL and OPG mRNA expression in osteoblastic MC3T3-E1 cells following treatment with conditioned MCF7 medium. ...
Iwasaka Kensuke - - 2011
We have recently reported that a low level of CO(2) laser irradiation induced growth stimulation (hormesis) of both human gingival fibroblast (HGF) and oral squamous cell carcinoma cell line (HSC-2), but the extent of hormetic response was much smaller than that previously reported for toxicants and radiation in other experimental ...
Ortenzi Vincenza - - 2011
The aim of this study is to investigate the role of the cell-cycle phase in cells exposed to radiation and chemicals in relation to the cellular response. The analysis was focused on the G2 cell-cycle phase, exploring the impact of p53 inhibition in human lymphocytes irradiated with X-rays in the ...
Chaudhry M Ahmad - - 2011
Abstract Nontargeted cellular effects by ionizing radiation are well documented. The bystander effect is a nontargeted phenomenon wherein the irradiated cells communicate and induce changes in nonirradiated cells. The nature of the bystander signal and how it impacts unirradiated cells remain to be elucidated. Examination of molecular changes could lead ...
Ghosh Somnath - - 2011
Carbon beams (5.16MeV/u, LET=290keV/μm) are high linear energy transfer (LET) radiation characterized by higher relative biological effectiveness than low LET radiation. The aim of the current study was to determine the signaling differences between γ-rays and carbon ion-irradiation. A549 cells were irradiated with 1Gy carbon or γ-rays. Carbon beam was ...
Shigetani Yoshimi - - 2011
This study aimed to clarify pulpal responses to gallium-aluminum-arsenide (GaAlAs) laser irradiation. Maxillary first molars of 8-week-old rats were irradiated at an output power of 0.5 or 1.5 W for 180 seconds, and the samples were collected at intervals of 0 to 14 days. The demineralized paraffin sections were processed ...
Ristić-Fira A - - 2011
Conventional radiotherapy with X- and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the ...
Terasaki Yasuhiro - - 2011
Molecular hydrogen (H(2)) is an efficient antioxidant that diffuses rapidly across cell membranes, reduces reactive oxygen species (ROS) such as hydroxyl radicals and peroxynitrite, and suppresses oxidative stress-induced injury in several organs. ROS have been implicated in radiation-induced damage to lungs. Because prompt elimination of irradiation-induced ROS should protect lung ...
Rebuzzini Paola - - 2011
This study aimed to investigate the cell cycle, apoptosis, cytogenetics and differentiation capacity of mouse embryonic stem cells (mESCs) that survived a single dose of 2 or 5 Gy γ-rays during a period of up to 96 h of culture. After 2 Gy irradiation and 24 h culture, compared to control, a ...
García-Contreras René - - 2011
Although we have recently established optimal experimental conditions of ultraviolet (UV)-irradiation for titanium plates (e.g. wavelength and exposure time) which enhanced osteoblast adhesion to the plates, the effects of UV-irradiation on cell structure are still unclear. Digital stereomicroscopy was used to investigate morphological alterations of non-stained viable and hematoxylin-eosin (HE)-stained ...
Autsavapromporn Narongchai - - 2011
Understanding the mechanisms that underlay the biological effects of particulate radiations is essential for space exploration and for radiotherapy. Here, we investigated the role of gap junction intercellular communication (GJIC) in modulating harmful effects induced in confluent cultures wherein most cells are traversed by one or more radiation tracks. We ...
Yen Hong-Wei - - 2011
A yeast, Rhodotorula glutinis, is regarded as a potential microbial oil producer, due to its high lipid content. The flask results of this study indicated that irradiation could increase the growth of R. glutinis compared to that of a batch without irradiation. Further 5-l fermenter results confirmed that irradiation could ...
Kuhmann Christine - - 2011
BACKGROUND AND PURPOSE: Repeated exposure to ionizing radiation (IR) can result in adaptive reactions. While DNA methylation changes in adaption to repeated stress exposure are established for a variety of drugs, their role in fractioned ionizing radiation is largely unknown. MATERIAL AND METHODS: MCF7 breast cancer cells were treated 5 ...
Chen Honghong - - 2011
PURPOSE: To investigate the difference of cellular response between low-dose-rate (LDR) (125)I seed irradiation and high-dose-rate (HDR) γ-irradiation in human lung cancer cells. METHODS AND MATERIALS: A549 and NCI-H446 cells with or without wortmannin (WM) treatment were exposed to (125)I seeds and γ-rays, respectively. Cell survival, micronuclei (MN) formation, and ...
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