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Yurdakok Begum B Department of Pharmacology and Toxicology, Ankara University Faculty of Veterinary Medicine, Ankara 06110, - - 2014
Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study ...
Kanan Y Y Department of Cell Biology, University of Oklahoma Health Sciences Center, BMSB 781, 940 Stanton L. Young Blvd., Oklahoma City, OK, 73104, - - 2014
Docosahexaenoic acid (DHA), an omega-3 fatty acid family member, is obtained by diet or synthesized from dietary essential omega-3 linolenic acid and delivered systemically to the choriocapillaris, from where it is taken up by the retinal pigment epithelium (RPE). DHA is then transported to the inner segments of photoreceptors, where ...
He Yuan Y Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, 167 Fang Dong Street, Xi'an, Shannxi, 710038, - - 2014
PURPOSE. We have previously shown that aging RPE cells are highly susceptible to oxidative stress, which impairs their mitochondrial function. This study investigates whether the neuroprotective PEDF can prevent mitochondrial pathology in aging RPE cells exposed to oxidative stress. METHODS. Human RPE cells were isolated from 50-75-year-old eyes and cultured ...
Lynge Martin E ME iNANO, Aarhus University, Gustav Wieds Vej 14, Aarhus, 8000, - - 2014
Implantable devices equipped with coatings which have the ability to carry and deliver active compounds are of great interest. We report the assembly of liposome-containing poly(dopamine) films, and their interaction with adhering cells. The liposome composition is varied by adding lipophilic dopamine-conjugates and charged lipids. The cell mean fluorescence (CMF) ...
Kim So-Jung SJ Department of Biochemistry, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea; MD-PhD program, Pusan National University School of Medicine, Yangsan 626-870, Republic of - - 2014
In the present study, we investigated whether celecoxib could induce the expression of NKG2D ligands in clonogenic colon cancer cells, and increase their susceptibility to NK cell-mediated cell death. Celecoxib and its non-coxib analogue, 2,5-dimethyl celecoxib, induced ULBP-1 and DR5 in both COX-2 negative HCT-15 cells and COX-2 positive HT-29 ...
Daubney James J School of Science and Technology, Nottingham Trent University, Nottingham, United - - 2014
Whilst mitotic rat embryonic cardiomyoblast-derived H9c2 cells have been widely used as a model system to study the protective mechanisms associated with flavonoids, they are not fully differentiated cardiac cells. Hence, the aim of this study was to investigate the cardioprotective and cardiotoxic actions of quercetin and two of its ...
Merabova Nana N Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, 3500 North Broad Street, 7th Floor, Philadelphia, PA, - - 2014
Autophagy is an evolutionarily conserved, selective degradation pathway of cellular components that is important for cell homeostasis under healthy and pathologic conditions. Here we demonstrate that an increase in the level of BAG3 results in stimulation of autophagy in glioblastoma cells. BAG3 is a member of a co-chaperone family of ...
Gall Jonathan M JM Renal Section - - 2014
Proximal tubule (PT) cells are critical targets of acute ischemic injury. Elimination of the mitochondrial fusion protein mitofusin 2 (Mfn2) sensitizes PT cells to apoptosis in vitro. However, the role of PT Mfn2 in ischemic AKI in vivo is unknown. To test its role, we evaluated the effects of conditional ...
Wang Shaoxiao S Departments of Biochemistry and Molecular Biology - - 2014
Phosphatidylserine decarboxylase, which is embedded in the inner mitochondrial membrane, synthesizes phosphatidylethanolamine (PE) and, in some cells, synthesizes the majority of this important phospholipid. Normal levels of PE can decline with age in the brain. Here we used yeast and worms to test the hypothesis that low levels of PE ...
Goutzourelas N N Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, - - 2014
A grape pomace extract enhanced antioxidant mechanisms in muscle and endothelial cells both in the absence and in the presence of oxidative stress-induced agent tert-butyl hydroperoxide (tBHP). In particular, muscle (C2C12) and endothelial (EA.hy926) cells were treated with the extract at noncytotoxic concentrations for 24 h, and the oxidative stress ...
Rudolf Emil E Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Králové, Šimkova 870, 500 38Hradec Králové, Czech Republic. Electronic address: - - 2014
Effects of chronic exposure to supranutritional sodium selenite (Se) were investigated in colonic fibroblasts. Initially, Se did not produce any gross changes in exposed cells, however basal levels of autophagy were transiently increased and p38 activity was stimulated. From the 3rd week onwards, Se decreased cell proliferation, with corrensponding changes ...
Tambe Dhananjay T DT Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts, United States of America. - - 2014
To quantify intercellular stresses in a cell sheet, Moussus et al. have recently proposed an approach which, under appropriate circumstances, may lead to significant simplification of the calculations required for stress recovery. Central to their approach is the assumption that the displacement fields of the substrate and the cells are ...
Shulyakova Natalya N Division of Genetics and Development Toronto Western Research Institute University Health Network, 399 Bathurst Street Toronto, Ontario, Canada M5T 2S8; Department of Physiology, University of Toronto Toronto, Ontario, Canada M5T - - 2014
Sirt3 is a mitochondrial sirtuin whose deacetylase activity regulates facets of oxidative metabolic efficiency, anti-oxidative capacity, and intra-mitochondrial signaling. In this study, we tested whether the over-expression of a human Sirt3-myc transgene in differentiated PC12 cells, a model of sympathetic catecholaminergic neurons, would affect the sensitivity of these cells to ...
Okada Minoru M Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, - - 2014
Type II collagen is a major component of cartilage. Heterozygous mutations in the type II collagen gene (COL2A1) result in a group of skeletal dysplasias known as Type II collagenopathy (COL2pathy). The understanding of COL2pathy is limited by difficulties in obtaining live chondrocytes. In the present study, we converted COL2pathy ...
Akcakaya Handan H Istanbul University, Istanbul Medical Faculty, Department of Biophysics, Istanbul, - - 2014
Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temporary arrest may ...
Law Courtney J CJ Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise, North Carolina Central University , Durham, North - - 2014
Abstract The underlying pathogenesis of type-II diabetes mellitus is in the dysfunction and selective loss of pancreatic islet β-cells, which ultimately leads to underproduction of endogenous insulin. Amylin, a 37-amino-acid human hormone that is cosecreted with insulin, helps regulate gastric emptying and maintain blood glucose homeostasis through improved postprandial satiety. ...
Wang Xiaoqing X Institute for Building Materials, ETH-Swiss Federal Institute of Technology Zurich, CH-8093 Zurich, Switzerland Applied Wood Materials Laboratory, EMPA-Swiss Federal Laboratories for Materials Science and Technology, CH-8600 Dübendorf, Switzerland Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, - - 2014
Bamboo is well known for its fast growth and excellent mechanical performance, but the underlying relationships between its structure and properties are only partially known. Since it lacks secondary thickening, bamboo cannot use adaptive growth in the same way as a tree would in order to modify the geometry of ...
Ventura Clara C Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, - - 2014
It is well known the participation of oxidative stress in the induction and development of different pathologies including cancer, diabetes, neurodegeneration and respiratory disorders among others. It has been reported that oxidative stress may be induced by pesticides and it could be the cause of health alteration mediated by pollutants ...
Sano Emiko E University of Montana; University of California, - - 2014
The origin of mutations under selection has been intensively studied using the Cairns system, in which cells of an E. coli lac mutant are plated on lactose and give rise to 100 Lac(+) revertants over several days. These revertants have been attributed variously to stress-induced mutagenesis of non-growing cells or ...
Park Min M Department of Biology, York University, Toronto, - - 2014
The association between obesity and heart failure is well documented and recent studies have indicated that understanding the physiological role of autophagy will be of great significance. Cardiomyocyte apoptosis is one component of cardiac remodeling which leads to heart failure and in this study we used palmitate-treated H9c2 cells as ...
Roh Jong-Lyel JL Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of - - 2014
Adaptation to cellular stress is not a vital function of normal cells but is required of cancer cells, and as such might be a sensible target in cancer therapy. Piperlongumine is a naturally occurring small molecule selectively toxic to cancer cells. This study assesses the cytotoxicity of piperlongumine and its ...
Muñoz-Fernández Raquel R Instituto de Parasitología y Biomedicina, Consejo Superior de Investigaciones Científicas, Armilla, Granada, - - 2014
Follicular dendritic cells (FDCs) present antigens to B cells in the lymphoid follicle and inhibit B-cell apoptosis. In previous work, we obtained human FDC lines that allowed us to study the antigen phenotype and functions of these cells, finding that they expressed α-smooth muscle (SM) actin (a protein involved in ...
Mews Philipp P Penn Epigenetics Program Department of Cell and Developmental - - 2014
Cell growth is attuned to nutrient availability to sustain homeostatic biosynthetic processes. In unfavorable environments, cells enter a non-proliferative state termed quiescence, but rapidly return to the cell cycle once conditions support energetic needs. Changing cellular metabolite pools are proposed to directly alter the epigenome via histone acetylation. Here we ...
Orlandi Viviana Teresa VT a Department of Theoretical and Applied Sciences , University of Insubria , Varese , - - 2014
Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 ...
Wu Qiong Q School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, P. R. China. - - 2014
Effective chemotherapy drugs for cancer that would inhibit tumor growth and suppress metastasis are currently lacking. In this study, a series of arene ruthenium complexes, [(η(6)-arene)Ru(H2iip)Cl]Cl (arene = p-cymene, ; CH3C6H5, ; and C6H6, ), were synthesized and their inhibitory activity against tumor cells were evaluated. The results showed that ...
Tiwari Pooja Munnilal PM Center for NanoBiotechnology Research and Department of Biological Sciences, Alabama State University, 1627 Hall Street, Montgomery, AL 36101, - - 2014
Reduced toxicity and ease of modification make gold nanoparticles (GNPs) suitable for targeted delivery, bioimaging and theranostics by conjugating cell-penetrating peptides (CPPs). This study presents the biodistribution and enhanced intracellular uptake of GNPs functionalized with VG-21, a CPP derived from vesicular stomatitis virus glycoprotein (G). Cell penetrating efficiency of VG-21 ...
Granato Marisa M Department of Experimental Medicine, "Sapienza" University of Rome, - - 2014
Autophagy is a catabolic pathway that helps cells to survive in stressful conditions. Cells also use autophagy to clear microbiological infections but, on the other hand, microbes have learned how to manipulate the autophagic pathway for their own benefit. The experimental evidence obtained in this study suggests that the autophagic ...
Shang Fengjun F Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, - - 2014
Pseudomonas aeruginosa uses a hierarchical cell-cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilitating early medical intervention. A recently isolated cell-cell communication molecule, a ...
Durga Mohan M Centre for Nanoscience and Technology, Anna University Chennai, Ac Tech Campus, Chennai 600025, Tamil Nadu, - - 2014
Air pollution has persistently been the major cause of respiratory-related illness and death. Environmental pollutants such as diesel and petrol exhaust particles (PEPs) are the major contributors to urban air pollution. The aim of the present study was to characterize and investigate the in vitro cytotoxicity, oxidative stress, DNA damage ...
Li Yanjie - - 2014
Beyond lipid-lowering effect, statins, the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase competitively inhibitors, have recently attracted wide concern on their anticarcinogenic properties in various cancer cells. However, the molecular mechanisms by which statins inhibit cancer cell proliferation remain unclear. In this study, we investigated the effect of simvastatin on cells proliferation ...
Li Yun Y a Department of Ophthalmology and Visual Sciences, the 2nd Xiangya Hospital, Central South University, 410011, Changsha, Hunan, - - 2014
This study evaluated the protective effect of alpha lipoic acid (ALA) in human adult retinal pigment epithelium cells (ARPE-19). An RPE oxidative stress model was established in ARPE-19 cells. Cell apoptosis was detected by Annexin V/PI staining and Hoechst33342 staining. Autophagy activation was evaluated by formation of acidic vesicular organelles ...
Rathore Sweta S GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, Faculty of Science, National University of Singapore , - - 2014
Abstract This work evaluated the feasibility of microencapsulating Clostridium acetobutylicum ATCC 824 cells by emulsification for fermentation to produce biobutanol. The effects of selected emulsification process on viability of the vegetative cells and spores were investigated to enable the selection of appropriate form of bacterium for immobilisation. The spores were ...
Chapman Lloyd A C LA Mathematical Institute, University of Oxford, Oxford, United Kingdom; Department of Computer Science, University of Oxford, Oxford, United - - 2014
The need for efficient and controlled expansion of cell populations is paramount in tissue engineering. Hollow fibre bioreactors (HFBs) have the potential to meet this need, but only with improved understanding of how operating conditions and cell seeding strategy affect cell proliferation in the bioreactor. This study is designed to ...
Nakata Keiji K Department of Emergency and Critical Care Medicine, Nippon Medical - - 2014
Mesenteric ischemia-reperfusion induces gut mucosal damage. Intestinal mucosal wounds are repaired by epithelial restitution. Although many different molecular mechanisms have been shown to affect cell metabolism under oxidative conditions, these molecular mechanisms and metabolic phenotypes are not well understood. Nuclear magnetic resonance (NMR) spectroscopic data can be used to study ...
Gu Yong Y School of Chinese Medicine, the University of Hong Kong, Hong Kong SAR, P. R. - - 2014
Site-2 protease (S2P) is a membrane-embedded protease that site-specifically cleaves intramembrane transcription factors, a necessary step for their maturation. S2P is well known to regulate cholesterol biosynthesis and endoplasmic reticulum stress in mammalian cells. In this study, we hypothesized that S2P could be responsible for the regulation of cellular oxidative ...
Ken Chuian-Fu CF Institute of Biotechnology, National Changhua University of Education, Changhua 500, Taiwan. - - 2014
Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative ...
Wang Hui H Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of China, Jinan University School of Medicine, Guangzhou 510632, Guangdong Province, China ; GHM Institute of CNS Regeneration, Jinan University, Guangzhou 510632, Guangdong Province, - - 2013
The death of retinal ganglion cells is a hallmark of many optic neurodegenerative diseases such as glaucoma and retinopathy. Oxidative stress is one of the major reasons to cause the cell death. Oligomeric proanthocyanidin has many health beneficial effects including antioxidative and neuroprotective actions. Here we tested whether oligomeric proanthocyanidin ...
Wu Lili L Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, - - 2013
The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) ...
van Teeseling Muriel C F - - 2013
Anammox bacteria convert ammonium and nitrite to dinitrogen gas under anaerobic conditions to obtain their energy for growth. The anammox reaction was deemed impossible until its discovery in the early 1990s. Now, anammox bacteria are recognized as major players in the global nitrogen cycle and estimated to be responsible for ...
Kriebel Katja - - 2013
Human mesenchymal stem cells (hMSCs) are multipotent by nature and are originally isolated from bone marrow. In light of a future application of hMSCs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study ...
Kristensen Dina G - - 2012
Testicular spermatocytic seminoma (SS) is a rare tumor type predominantly found in elderly men. It is thought to originate from spermatogonia and shows cytological and genetic heterogeneity. In this study, we performed for the first time a comprehensive analysis of epigenetic modifications in a series of 36 SS samples. We ...
Hodson David J - - 2011
The pulsatile secretion of hormones from the mammalian pituitary gland drives a wide range of homeostatic responses by dynamically altering the functional set-point of effector tissues. To accomplish this, endocrine cell populations residing within the intact pituitary display large-scale changes in coordinated calcium-spiking activity in response to various hypothalamic and ...
Monahan Pamela P Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, - - 2012
A delicate balance between proliferation and differentiation must be maintained in the developing pituitary to ensure the formation of the appropriate number of hormone producing cells. In the adult, proliferation is actively restrained to prevent tumor formation. The cyclin dependent kinase inhibitors (CDKIs) of the CIP/KIP family, p21, p27 and ...
Yako Hideji - - 2011
Pituitary embryonic development progresses daily toward terminal differentiation exhibiting quantitative and qualitative alterations regulated by signal molecules and transcription factors expressed under temporospatial control. In this study, we analyzed the heterogeneity of the cells in the pituitary primordium of embryonic day (E) 13.5. The three-dimensional structure of the Rathke's pouch ...
Yoshida S - - 2011
We reported recently that a pituitary-specific transcription factor PROP1 is present in SOX2-positive cells and disappears at the early stage of the transition from progenitor cell to committed cell during the embryonic development of the rat pituitary. In the present study, we examined the localisation and identification of SOX2-positive and ...
Liu James K - - 2011
R-flurbiprofen, a nonsteroidal anti-inflammatory drug derivative, has been shown to inhibit colonic adenoma formation in mice. We investigated the effects of R-flurbiprofen on cell proliferation and apoptosis in pituitary adenoma cell lines. GH4C1 rat pituitary cell line cultures and low-passage human primary pituitary cell cultures were treated with varying concentrations ...
Zhao Weijiang - - 2011
The binding of Neuregulin-1 (Nrg1) to its cognate receptors ErbB-3 and -4 mediates intercellular and intracellular communication. In vitro, this interaction has been shown to control prolactin (PRL) secretion from pituitary tumour cells. However, Nrg1/ErbB signaling and its function in vivo are not well understood. In this study, we demonstrated ...
Garrel Ghislaine - - 2011
The activity of pituitary gonadotrope cells, crucial for reproductive function, is regulated by numerous factors including signals related to nutritional status. In this work, we demonstrated, for the first time, that in vivo central exposure of rats to lipids intracarotid infusion of a heparinized triglyceride emulsion selectively increases the expression ...
Kikuchi Motoshi - - 2011
In the anterior and intermediate lobes of the rat pituitary gland, non-hormone-producing cells that express S-100 protein coexist with various types of hormone-producing cells and are believed to function as phagocytes, supporting and paracrine-controlling cells of hormone-producing cells and stem cells, among other functions; however, their cytological characteristics are not ...
Puertas-Avendaño R A - - 2011
To better understand the role of the non-canonical Notch ligand delta-like protein 1 (DLK1), in hormone-producing cells, we studied the cell distribution and subcellular localisation of DLK1 in the pituitary of male adult 129/SvJ mice, and analysed the variations in the hormone-producing cells associated with the lack of this gene ...
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