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Wu Ryan T Y RT University of Maryland, United - - 2014
Oxidative stress and persistent DNA damage response contribute to cellular senescence, a degeneration process critically involving ataxia telangiectasia mutated (ATM) and p53. Selenoprotein H (SelH), a nuclear selenoprotein, is proposed to carry redox and transactivation domains. To determine the role of SelH in genome maintenance, shRNA knockdown was employed in ...
Martin Shaun S Northern Institute for Cancer Research, Medical School, Newcastle University, NE2 4HH, - - 2014
Glucose-regulated protein 78 (GRP78) is a stress sensor which interacts with unfolded protein response (UPR) activators in the endoplasmic reticulum (ER). The aim of this study was to test the hypothesis that GRP78 has distinct functional roles in mediating the effects of ER stress in neuroblastoma compared to other neuroectodermal ...
Ooi Theng Choon TC Biomedical Science Programme, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, - - 2014
The aim of this study is to investigate the ability of zinc carnosine to protect the human lymphoblastoid (WIL2-NS) cell line from hydrogen peroxide-induced DNA damage. Cells were cultured with medium containing zinc carnosine at the concentrations of 0.4, 4, 16 and 32 μM for 9 days prior to treatment with 30 μM ...
Cheng Yu-Chi YC Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, - - 2014
Endoplasmic reticulum stress occurs in a variety of patho-physiological mechanisms and there has been great interest in managing this pathway for the treatment of clinical diseases. Autophagy is closely interconnected with endoplasmic reticulum stress to counteract the possible injurious effects related with the impairment of protein folding. Studies have shown ...
Cai Meng M State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , P.R. - - 2014
Abstract Purpose: To investigate the effects of the mitochondria-targeted peptide, SS31, on cultured human lens epithelial cells (HLECs) under conditions of oxidative stress. Methods: Optimal concentrations of SS31 were determined by MTT assay. HLEB-3 cells were first treated with SS31 for 2 h and then with 500 µM t-BHP for 6 h. Cell ...
Campelo Riward R Instituto de Estudios Avanzados, Centro de Biotecnologia, MppEUCT, Caracas, - - 2014
In its canonical role the reverse transcriptase telomerase recovers the telomeric repeats that are lost during the DNA replication. In mammalian cells other locations and activities have been recently described for the telomerase protein subunit TERT. In the present work using biochemistry, molecular biology and electron microscopy techniques, we have ...
Rayen Ine I School for Mental Health and Neuroscience, Department of Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, - - 2014
During pregnancy and postpartum period, 20 % of women are affected by depression, which is a growing health concern. Selective serotonin reuptake inhibitor (SSRI) medications are popular treatments for maternal depression; however, the effect of maternal depression and perinatal SSRI exposure on offspring's neural development needs further investigation. This study aims ...
Zheng Yong Y Department of Anatomy and Embryology, Wuhan University School of Medicine, Wuhan, Hubei 430071, P.R. - - 2014
Following a limited number of cell divisions, mesenchymal stem cells (MSCs) undergo senescence, and these senescent cells maintain metabolic modification and remain viable for long periods. Autophagy, an intracellular bulk degradation process, provides a survival effect for cells under stress. In this study, the effect of autophagy on senescent MSCs ...
Zhang Qiu-Xiang QX State Key Laboratory of Food Science and Technology, School of Food Science and Technology,Jiangnan University,1800 Lihu Avenue, Wuxi, Jiangsu - - 2014
The relationship between hydrophobicity and the protective effect of whey protein hydrolysates (WPHs) against oxidative stress was studied. Whey protein was first hydrolysed by pepsin and trypsin to obtain WPHs. After absorbed by macroporous adsorption resin DA201-C, three fractions named as M20, M40, and M60 were eluted by various concentrations ...
Sun Hongli H Shaanxi Institute of Pediatric Diseases, The Affiliated Children Hospital of Xi'an Jiaotong University College of Medicine, Shaanxi 86-710003, PR - - 2014
To clarify the alterations of proliferation and differentiation of hippocampal cells in prenatally stressed rats. We investigated the impact of prenatal restraint stress on the hipocampal cell proliferation in the progeny with 5-bromo-2'-deoxyuridine (BrdU), which is a marker of proliferating cells and their progeny. In addition, we observed the differentiation ...
Bradley Eric E Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, 1120 15th Street, Augusta, GA, 30912, - - 2014
Stress adaptation effect provides cell protection against ischemia induced apoptosis. Whether this mechanism prevents other types of cell death in stroke is not well studied. This is an important question for regenerative medicine to treat stroke since other types of cell death such as necrosis are also prominent in the ...
Kim Eun-Mee EM Department of Emergency Medical Technology, Korea Nazarene University, Cheonan 331-718, South - - 2014
We have previously demonstrated that matrix metalloprotease-3 (MMP-3) can act inside the cell to trigger apoptosis in response to various cell stresses in dopaminergic neuronal cells. However, the mechanism by which MMP-3 activity leads to caspase-3 activation in apoptotic signaling was not known. In the present study, we found that ...
Kawase Tomoyuki T Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan; Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan. Electronic address: - - 2014
For successful cell transplantation therapy, the quality of cells must be strictly controlled. Unfortunately, to exclude inappropriate cells that possess structurally abnormal chromosomes, currently only karyotyping functions as an assessment. Unfortunately, this methodology is time-consuming and only effective for metaphasic cells. To develop a more efficient, inclusive and sensitive methodology, ...
Tran Kim M - - 2014
Endoplasmic reticulum (ER) stress plays an important role in the decline in pancreatic β cell function and mass observed in type 2 diabetes. Here, we developed a novel β cell-based high-throughput screening assay to identify small molecules that protect β cells against ER stress-induced cell death. Mouse βTC6 cells were ...
Parplys Ann C AC Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United - - 2014
RAD51-associated protein 1 (RAD51AP1) is critical for homologous recombination (HR) by interacting with and stimulating the activities of the RAD51 and DMC1 recombinases. In human somatic cells, knockdown of RAD51AP1 results in increased sensitivity to DNA damaging agents and to impaired HR, but the formation of DNA damage-induced RAD51 foci ...
Yurdakok Begum B Department of Pharmacology and Toxicology, Ankara University Faculty of Veterinary Medicine, Ankara 06110, - - 2014
Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study ...
Wang Xuan X Department of Medical Cell Biology, Uppsala University , Uppsala , - - 2014
Abstract Background. Sodium palmitate causes apoptosis of β-cells, and the anti-apoptotic protein Bcl-2 has been shown to counteract this event. However, the exact mechanisms that underlie palmitate-induced pancreatic β-cell apoptosis and through which pathway Bcl-2 executes the protective effect are still unclear. Methods. A stable Bcl-2-overexpressing RINm5F cell clone (BMG) ...
Batnasan Enkhzaya E Key Laboratory of Molecular Epigenetics of the Ministry of Education, Changchun, 130024, - - 2014
Oxidative stress-induced DNA damage results in over-activation of poly(ADP-ribose) polymerase 1 (PARP1), leading to parthanatos, a newly discovered cell elimination pathway. Inhibition of PARP1-dependent cell death has shown to improve the outcome of diseases, including stroke, heart ischemia, and neurodegenerative diseases. In the present study we aimed to detect whether ...
McKinnon D D DD Department of Chemical and Biological Engineering, BioFrontiers Institute, University of Colorado, Boulder, Colorado 80303, - - 2014
Studies focused on understanding the role of matrix biophysical signals on cells, especially those when cells are encapsulated in hydrogels that are locally remodelled, are often complicated by appropriate methods to measure differences between the bulk and local material properties. From this perspective, stress-relaxing materials that allow long-term culture of ...
Solé Carme C Cell Signaling unit, Departament de Ciències Experimentals i de la Salut, Cell Signaling Research Group, Universitat Pompeu Fabra (UPF), Dr Aiguader 88, E-08003, Barcelona, - - 2014
Eukaryotic cells have developed sophisticated systems to constantly monitor changes in the extracellular environment and to orchestrate a proper cellular response. To maximize survival, cells delay cell-cycle progression in response to environmental changes. In response to extracellular insults, stress-activated protein kinases (SAPKs) modulate cell-cycle progression and gene expression. In yeast, ...
Liu Fei F 1State Key Laboratory of Mechanical Transmission,College of Mechanical Engineering,Chongqing University,Chongqing - - 2014
Mechanical properties are vital for living cells, and various models have been developed to study the mechanical behavior of cells. However, there is debate regarding whether a cell behaves more similarly to a "cortical shell - liquid core" structure (membrane-like) or a homogeneous solid (cytoskeleton-like) when experiencing stress by mechanical ...
Savion Naphtali N Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel Aviv University, Sheba Medical Center, Tel Hashomer 52621, Israel. Electronic address: - - 2014
Oxidative stress and/or low cellular glutathione are associated with development and progression of neurodegenerative diseases. We have shown that S-allylmercapto-N-acetylcysteine (ASSNAC) up-regulates the level of glutathione and phase II detoxifying enzymes in cultured vascular endothelial cells. The present study demonstrates that exposure of nerve cell lines to ASSNAC significantly increases ...
Banerjee Priyanjalee P Department of Biochemistry, Institute of Postgraduate Medical Education and Research, 244, AJC Bose Road, Kolkata, 700020, - - 2014
The increased accumulation of iron in the brain in Alzheimer's disease (AD) is well documented, and excess iron is strongly implicated in the pathogenesis of the disease. The adverse effects of accumulated iron in AD brain may include the oxidative stress, altered amyloid beta-metabolism and the augmented toxicity of metal-bound ...
Lin Chia-Yu CY Department of Health and Nutrition Biotechnology, Asia University , Taichung, Taiwan - - 2014
Abstract Oxidative stress has been implicated in the etiology of pancreatic β-cell dysfunction and diabetes. Studies have shown that chlorella could be important in health promotion or disease prevention through its antioxidant capacity. However, whether chlorella has a cytoprotective effect in pancreatic β-cells remains to be elucidated. We investigated the ...
Hariharan V V Department of Biomedical Engineering, Columbia University, New York, - - 2014
The majority of patients diagnosed with arrhythmogenic right ventricular cardiomyopathy (ARVC) have mutations in genes encoding desmosomal proteins, raising the possibility that abnormal intercellular adhesion plays an important role in disease pathogenesis. We characterize cell mechanical properties and molecular responses to oscillatory shear stress in cardiac myocytes expressing mutant forms ...
Xia Meihui M Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. - - 2014
The mechanisms underlying cisplatin resistance in tumors are not fully understood. Previous studies have reported that cellular resistance to oxidative stress is accompanied by resistance to cisplatin. However, the relationship between the resistance to oxidative stress and cisplatin drug resistance in human ovarian cancer cells (HOCCs) is not clear. Here, ...
Kabiraj Parijat - - 2014
Nitrosative stress mediated S-nitrosylation (SNO) of protein disulfide isomerase (PDI), a housekeeping oxidoreductase, has been implicated in the pathogenesis of sporadic Parkinson's (PD) and Alzheimer's (AD) diseases. Previous cell line studies have indicated that SNO-PDI formation provokes synphilin-1 aggregation, the minor Parkinsonian biomarker protein. Yet no work exists investigating whether ...
Wang W W Institute of Urology, Huashan Hospital, Fudan University, Shanghai, - - 2014
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role of PPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were ...
Song Kibbeum K Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk, 561-156, Republic of - - 2014
Alcoholism, which refers to the excessive consumption of alcohol, has deleterious effects on personal and social health worldwide. Oxidative stress evoked by ethanol plays an important role in the pathogenesis of neurodegenerative diseases. Rutin is a bioflavonoid that has been demonstrated to scavenge superoxide radicals. However, the effects of rutin ...
Gorelova Olga O Department of Bioengineering Faculty of Biology, Moscow State University, GSP-1, 119234, Moscow, - - 2014
A quantitative micromorphometric study of the cell compartment rearrangements was performed in a symbiotic chlorophyte Desmodesmus sp. 3Dp86E-1 grown on nitrogen (N) replete or N-free medium under 480 μmol PAR quanta m(-2) s(-1). The changes in the chloroplast, intraplastidial, and cytoplasmic inclusions induced by high light (HL) and N starvation were similar ...
Dasmahapatra Girija G Internal Medicine, Virginia Commonwealth - - 2014
Interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor Carfilzomib (CFZ) were examined in non-Hodgkin's lymphoma models, including diffuse large B-cell (DLBCL), mantle cell (MCL) and double-hit lymphoma cells. Marked in vitro synergism was observed in multiple cell types associated with activation of cellular stress pathways (e.g., ...
Ghandhi Shanaz A - - 2014
Radiation induced bystander effects are an important component of the overall response of cells to irradiation and are associated with human health risks. The mechanism responsible includes intra-cellular and inter-cellular signaling by which the bystander response is propagated. However, details of the signaling mechanism are not well defined. We measured ...
Zhong Bin B Department of Bioscience, College of life science, Nanchang University, Nanchang 330031, - - 2014
Heavy metal ion is one of the critical environmental pollutants accumulated in living organisms and causes toxic or carcinogenic effects once passed threshold levels. As an important member of Hsp70 (heat shock protein 70) family, the 78-kDa glucose-regulated protein (GRP78) can enhance cell survival rates remarkably under thermal stress. Recent ...
McGoldrick Christopher A - - 2014
Cancer cell esterases are often overexpressed and can have chiral specificities different from that of the corresponding normal cells and can, therefore, be useful targets for activating chemotherapeutic prodrug esters. Prodrug esters are inactive compounds that can be preferentially activated by esterase enzymes. Moreover, cancer cells often exhibit a high ...
Urano Daisuke D Department of Biology at the University of North Carolina, Chapel Hill, NC, 27599-3280, - - 2014
The plant G-protein network, comprising Gα, Gβ, and Gγ core subunits, regulates development, senses sugar, and mediates biotic and abiotic stress responses. Here, we report G-protein signalling in the salt stress response using two crop models, rice and maize. Loss-of-function mutations in the corresponding genes encoding the Gα subunit attenuate ...
Blum Barak B Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, United - - 2014
Dysfunction or death of pancreatic β cells underlies both types of diabetes. This functional decline begins with β cell stress and de-differentiation. Current drugs for T2D lower blood glucose levels, but they do not directly alleviate β cell stress nor prevent, let alone reverse, β cell de-differentiation. We show here ...
Giri Sib Sankar SS Dept. of Biotechnology, Periyar Maniammai University, Thanjavur-613403, Tamil Nadu, India. Electronic address: - - 2014
To understand the function of HSP70 of Labeo rohita (LrHSP70) in cellular protection, LrHSP70 ORF cDNA was inserted into the plasmid of pET-32a(+) or pEGFP-L1. Then, the recombinant plasmids were transformed or transfected into E. coli cells, mouse myeloma cells (MPC-11) or fish hepatoma cells (PLHC-1). Western blot results revealed ...
Zhou Shi-Wei SW Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. - - 2014
Simple hepatic steatosis is the early stage of non‑alcoholic fatty liver disease and is recognized as a benign process. Previous studies show that glucagon‑like peptide‑1 has great potential in improving hepatic steatosis. Recent data have revealed that inhibiting autophagy exacerbates lipid accumulation in hepatocytes. Therefore, the present study aimed to ...
Salleron Lisa L Department of Human Protein Sciences, Faculty of Medicine, University of Geneva. Electronic address: - - 2014
Deoxyribose-phosphate aldolase (EC 4.1.2.4), which converts 2-deoxy-D-ribose-5-phosphate into glyceraldehyde-3-phosphate and acetaldehyde, belongs to the core metabolism of living organisms. It was previously shown that human cells harbor deoxyribose phosphate aldolase activity but the protein responsible of this activity has never been formally identified. This study provides the first experimental evidence ...
Kanan Y Y Department of Cell Biology, University of Oklahoma Health Sciences Center, BMSB 781, 940 Stanton L. Young Blvd., Oklahoma City, OK, 73104, - - 2014
Docosahexaenoic acid (DHA), an omega-3 fatty acid family member, is obtained by diet or synthesized from dietary essential omega-3 linolenic acid and delivered systemically to the choriocapillaris, from where it is taken up by the retinal pigment epithelium (RPE). DHA is then transported to the inner segments of photoreceptors, where ...
Sieroslawska Anna A Department of Physiology and Ecotoxicology, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708, Lublin, - - 2014
Cylindrospermopsin (CYN) is a cyanotoxin detected in water reservoirs worldwide. The toxin is a potent protein synthesis inhibitor capable of adversely influencing a wide range of cell functions. While data on the prooxidative potency of CYN are inconsistent, genotoxic effects towards certain mammalian cell types have been described. However, such ...
He Yuan Y Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, 167 Fang Dong Street, Xi'an, Shannxi, 710038, - - 2014
PURPOSE. We have previously shown that aging RPE cells are highly susceptible to oxidative stress, which impairs their mitochondrial function. This study investigates whether the neuroprotective PEDF can prevent mitochondrial pathology in aging RPE cells exposed to oxidative stress. METHODS. Human RPE cells were isolated from 50-75-year-old eyes and cultured ...
Lynge Martin E ME iNANO, Aarhus University, Gustav Wieds Vej 14, Aarhus, 8000, - - 2014
Implantable devices equipped with coatings which have the ability to carry and deliver active compounds are of great interest. We report the assembly of liposome-containing poly(dopamine) films, and their interaction with adhering cells. The liposome composition is varied by adding lipophilic dopamine-conjugates and charged lipids. The cell mean fluorescence (CMF) ...
Kim So-Jung SJ Department of Biochemistry, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea; MD-PhD program, Pusan National University School of Medicine, Yangsan 626-870, Republic of - - 2014
In the present study, we investigated whether celecoxib could induce the expression of NKG2D ligands in clonogenic colon cancer cells, and increase their susceptibility to NK cell-mediated cell death. Celecoxib and its non-coxib analogue, 2,5-dimethyl celecoxib, induced ULBP-1 and DR5 in both COX-2 negative HCT-15 cells and COX-2 positive HT-29 ...
Daubney James J School of Science and Technology, Nottingham Trent University, Nottingham, United - - 2014
Whilst mitotic rat embryonic cardiomyoblast-derived H9c2 cells have been widely used as a model system to study the protective mechanisms associated with flavonoids, they are not fully differentiated cardiac cells. Hence, the aim of this study was to investigate the cardioprotective and cardiotoxic actions of quercetin and two of its ...
Merabova Nana N Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, 3500 North Broad Street, 7th Floor, Philadelphia, PA, - - 2014
Autophagy is an evolutionarily conserved, selective degradation pathway of cellular components that is important for cell homeostasis under healthy and pathologic conditions. Here we demonstrate that an increase in the level of BAG3 results in stimulation of autophagy in glioblastoma cells. BAG3 is a member of a co-chaperone family of ...
Gall Jonathan M JM Renal Section - - 2014
Proximal tubule (PT) cells are critical targets of acute ischemic injury. Elimination of the mitochondrial fusion protein mitofusin 2 (Mfn2) sensitizes PT cells to apoptosis in vitro. However, the role of PT Mfn2 in ischemic AKI in vivo is unknown. To test its role, we evaluated the effects of conditional ...
Wang Shaoxiao S Departments of Biochemistry and Molecular Biology - - 2014
Phosphatidylserine decarboxylase, which is embedded in the inner mitochondrial membrane, synthesizes phosphatidylethanolamine (PE) and, in some cells, synthesizes the majority of this important phospholipid. Normal levels of PE can decline with age in the brain. Here we used yeast and worms to test the hypothesis that low levels of PE ...
Goutzourelas N N Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, - - 2014
A grape pomace extract enhanced antioxidant mechanisms in muscle and endothelial cells both in the absence and in the presence of oxidative stress-induced agent tert-butyl hydroperoxide (tBHP). In particular, muscle (C2C12) and endothelial (EA.hy926) cells were treated with the extract at noncytotoxic concentrations for 24 h, and the oxidative stress ...
Koppenhöfer D D Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, Zweibrücken, - - 2014
Dementia is one of the big medical challenges of our time with Alzheimer's, Huntington's and Parkinson's disease among its most common forms. In year 2000, 4.5 million people were diagnosed with Alzheimer's disease in the United States. In the case of Alzheimer's disease one of many contributing factors is a ...
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