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Nair Rashmi R RR REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, - - 2014
The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the ...
Tsuji Tadahiro T Otorhinolaryngology, Akita University School of - - 2014
Agr2 is a member of the endoplasmic reticulum (ER) protein disulfide isomerase, which physiologically regulates protein folding and plays a pivotal role in resistance to ER stress. Agr2 is expressed primarily in adenocarcinomas of various organs, and Agr2 protein overexpression participates in neoplastic transformation and metastasis, therefore acts as a ...
Kang Ji Sook JS Blue‑Bio Industry RIC and Anti‑Aging Research Center, Dongeui University, Busan 614-714, Republic of - - 2014
The aim of the present study was to examine the cytoprotective effects of Schisandrae semen essential oil (SSeo), purified from Schisandrae fructus, against oxidative stress‑induced cell damage in C2C12 myoblasts. SSeo attenuated hydrogen peroxide (H2O2)‑induced growth inhibition and exhibited scavenging activity against the intracellular reactive oxygen species (ROS) that were ...
Delarue Morgan M Institut Curie , UMR 168 (CNRS, UPMC Univ Paris 6), 26 rue d'Ulm, 75005 Paris , - - 2014
We discuss the short-time response of a multicellular spheroid to an external pressure jump. Our experiments show that 5 min after the pressure jump, the cell density increases in the centre of the spheroid but does not change appreciably close to the surface of the spheroid. This result can be ...
Ishii Takashi T Frontier Research Center and Department of Biochemistry, Fukuoka Dental College, Fukuoka 814-0193, - - 2014
In aerobically growing cells, in which reactive oxygen species are produced, the guanine base of RNA is oxidized to 8-oxo-7,8-dihydroguanine, which induces alterations in the gene expression. Here we show that the human Auf1 protein, also called HNRNPD, binds specifically to RNA containing this oxidized base and may be involved ...
Davis Angela L AL University of Arizona, United - - 2014
Pharmacological induction of proteotoxic stress is rapidly emerging as a promising strategy for cancer cell-directed chemotherapeutic intervention. Here, we describe the identification of a novel drug-like heat shock response inducer for the therapeutic induction of proteotoxic stress targeting malignant human melanoma cells. Screening a focused library of compounds containing redox-directed ...
Porter Kristine K Duke University, Department of Ophthalmology, Durham, NC, - - 2014
Primary open angle glaucoma (POAG) is a degenerative disease commonly associated with aging and elevated intraocular pressure (IOP). Higher resistance to aqueous humor (AH) outflow through the trabecular meshwork (TM) generates the elevated IOP in POAG; unfortunately the underlying molecular mechanisms responsible for elevated resistance are unknown. It is widely ...
Cierlitza Monika M The Saarland University Hospital, Department of Dermatology, D-66421, Homburg/Saar, - - 2014
Despite recent success in melanoma therapy, most patients with metastatic disease still undergo deadly progression. We have identified a novel mechanism of multi-drug resistance allowing a small subpopulation of slow-cycling melanoma cells to survive based on elevated oxidative bioenergy. In the present study, we asked whether such slow-cycling cells could ...
Aung Aereas A Department of Bioengineering, University of California-San Diego, La Jolla, - - 2014
Cell invasion and migration that occurs, for example, in cancer metastasis is rooted in the ability of cells to navigate through varying levels of physical constraint exerted by the extracellular matrix. Cancer cells can invade matrices in either a protease-independent or a protease-dependent manner. An emerging critical component that influences ...
Dmitriev Ruslan I RI School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: - - 2014
Inhibitors of histone deacetylases are frequently used against ischemia-induced injury, but the specific mechanisms of their action are poorly understood. Here, we report that following a 5-7-hour oxygen-glucose deprivation (OGD) acetylation of histone H4 at residue K16 (H4K16Ac) decreases by 40-80% in both PC12 cells and primary neurons. This effect ...
Scarlet D D Centre for Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, - - 2014
In contrast to other domestic animal species, in vitro maturation (IVM) of oocytes in the horse is still not successful. Oocytes for IVM are obtained either from slaughterhouse ovaries or via ovum pick-up from living mares. Both situations may be associated with a stress-induced glucocorticoid release. So far, neither an ...
Toh Wei Seong WS Faculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, Singapore 119083, Singapore; Tissue Engineering Program, Life Sciences Institute, National University of Singapore, 27 Medical Drive, Singapore 117510, Singapore. Electronic address: - - 2014
Hydrogels are natural or synthetic polymer networks that have high water-absorbing capacity and closely mimic native extracellular matrices. As hydrogel-based cell delivery systems are being increasingly employed in regenerative medicine, several advances have been made in the hydrogel chemistry and modification for enhanced control of cell fate and functions, and ...
Gu Shiyan S Department of Environmental Health, West China School of Public Health, Sichuan University, No. 16, Section 3, Renmin Nan Road, Chengdu, 610041, People's Republic of - - 2014
Arsenic trioxide (As2O3) is a potent anticancer drug for the treatment of acute promyelocytic leukemia. However, the clinical applications of the agent to treat solid tumors are largely compromised by the drug resistance. Our previous study demonstrated that resveratrol, a plant-derived natural product, could potentiate the toxicity of arsenite in ...
Yan Yanqin - - 2014
BackgroundOxidative stress causes the defects of retinal pigment epithelial (RPE) cells that contribute to age-related macular degeneration (AMD). This study was conducted to determine whether wogonin could prevent H2O2-induced oxidative stress in RPE cells.MethodsA RPE cell line, ARPE-19, was obtained for the cell model. ARPE-19 cells were pre-treated with various ...
Vrček Ivana Vinković IV Institute for Medical Research and Occupational Health, Analytical Toxicology and Mineral Metabolism Unit, Ksaverska cesta 2, 10 000, Zagreb, - - 2014
Scientific information on the potential harmful effects of silver nanoparticles (AgNPs) on human health severely lags behind their exponentially growing applications in consumer products. In assessing the toxic risk of AgNP usage, liver, as a detoxifying organ, is particularly important. The aim of this study was to explore the toxicity ...
Esposito Davide D Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II", Naples 80131, Italy. Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, - - 2014
Recent evidence showed that a variety of DNA damaging agents including 5-FU and L-OHP impairs ribosomal biogenesis activating a ribosomal stress pathway. Here, we demonstrate that in lung and colon cancer cell lines devoid of p53, the efficacy of 5-FU and L-OHP chemotherapy depends on rpL3 status. Specifically, we demonstrate ...
Chiu Hui-Wen HW Department of Environmental and Occupational Health, National Cheng Kung University, Tainan, Taiwan. - - 2014
Nanoparticles (NPs) have been used to produce a wide range of products that have applications in imaging and drug delivery in medicine. Due to their chemical stability, well-controlled sizes and surface charges, polystyrene (PS) NPs have been developed as biosensors and drug delivery carriers. However, the possible adverse biological effects ...
Hayashi Ken K Department of Otolaryngology, Kamio Memorial Hospital, Tokyo, 101-0063, Japan; Department of Otolaryngology, School of Medicine, Keio University, Tokyo, 160-8582, Japan. Electronic address: - - 2014
The main purposes of our study were to consider the effect of autophagy on auditory cells under oxidative stress, and the function of possible cross talk among p62, Keap1 and Nrf2 in autophagy-deficient auditory cells. First, we described how cell death was induced in auditory cell line (HEI-OC1) exposed to ...
Anekal Praju Vikas PV Institute of Molecular and Cell Biology, - - 2014
Cell migration requires dynamic remodelling of the actomyosin network. We report here that an adapter protein, ArgBP2 is a component of α-actinin containing stress fibres and inhibits migration. ArgBP2 is undetectable in many commonly studied cancer derived cell lines. COS-7 and HeLa cells express ArgBP2 (by western analysis), but expression ...
Moriya Shota S Department of Biochemistry, Tokyo Medical University, Tokyo, - - 2014
The inhibitory effects of macrolide antibiotics including clarithromycin (CAM) on autophagy flux have been reported. Although a macrolide antibiotic exhibits no cytotoxicity, its combination with bortezomib (BZ), a proteasome inhibitor, for the simultaneous blocking of the ubiquitin (Ub)‑proteasome and autophagy‑lysosome pathways leads to enhanced multiple myeloma (MM) cell apoptosis induction via stress overloading of ...
Guo Jun J Department of Biochemistry, Nanjing Medical University, Nanjing, Jiangsu 210029, - - 2014
Cell mechanics plays a role in stem cell reprogramming and differentiation. To understand this process better, we created a genetically encoded optical probe, named actin-cpstFRET-actin (AcpA), to report forces in actin in living cells in real time. We showed that stemness was associated with increased force in actin. We reprogrammed ...
Hosomi Shuhei S aDivision of Gastroenterology, Hepatology, and Endoscopy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA bDivision of Gastroenterology and Hepatology, Department of Medicine, Addenbrooke's Hospital, University of Cambridge, Cambridge, - - 2014
The purpose of this study is to provide an overview of the role of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) in inflammatory bowel disease (IBD). Human genetic studies have identified several UPR-related genes and autophagy-related genes as IBD risk loci. Impairment of each branch of the ...
Pretor S - - 2014
In vitro studies of ocular bioavailability of active pharmaceutical ingredients (API) from colloidal drug delivery systems do not consider physiological shear stress generated by eyelid wiping and tear flow. The present study introduces a live cell imaging approach which enables the investigation of model drug uptake from various formulations under ...
Chen Hongbo H College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. - - 2014
The aim of the present study was to investigate the association between heat shock protein (Hsp) 70 expression kinetics and heat stress‑induced damage to rat myocardial cells in vitro and in vivo. The results showed that the activity of heart injury‑associated enzymes, including aspartate aminotransferase and creatine kinase, significantly increased and myocardial cells ...
Gorasia Dhana Govind - - 2014
The complex interplay of many cell types and the temporal heterogeneity of pancreatic islet composition obscures the direct role of resident alpha and beta cells in the development of Type 1 diabetes. Therefore, in addition to studying islets isolated from Non-Obese Diabetic mice, we analysed homogenous cell populations of murine ...
Siddiqui Maqsood A MA Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; Al-Jeraisy Chair for DNA Research, King Saud University, Riyadh, Saudi Arabia. Electronic address: - - 2014
The present investigation was aimed to study the cytotoxicity, oxidative stress, and genotoxicity induced by molybdenum nanoparticles (Mo-NPs) in mouse skin fibroblast cells (L929). Cells were exposed to different concentrations (1-100μg/ml) of Mo-NPs (size 40nm) for 24 and 48h. After the exposure, different cytotoxicity assays (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, ...
Du Zhe Z Department of Civil Engineering, University of Nebraska-Lincoln, - - 2014
Despite the low nutrient level and constant presence of secondary disinfectants, bacterial re-growth still occurs in drinking water distribution systems. The molecular mechanisms that starved bacteria use to survive low-level chlorine-based disinfectants are not well understood. The objective of this study is to investigate these molecular mechanisms at the protein ...
Webster Marie R MR The Wistar Institute, Philadelphia, PA, - - 2014
We have previously shown that Wnt5A drives invasion in melanoma. We have also shown that Wnt5A promotes resistance to therapy designed to target the BRAF(V600E) mutation in melanoma. Here, we show that melanomas characterized by high levels of Wnt5A respond to therapeutic stress by increasing p21 and expressing classical markers ...
Hsiung Jamie J Departments of Pathology and Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California, - - 2014
Oxidative stress-mediated injury to the retinal pigment epithelium (RPE) is a major factor involved in the pathogenesis of age-related macular degeneration (AMD), the leading cause of blindness in the elderly. Human embryonic stem cell (hESC)-derived RPE cells are currently being evaluated for their potential for cell therapy in AMD patients ...
Polo M M Departamento de Farmacología, Facultad de Medicina, Universitat de Valencia, Valencia, Spain; FISABIO-Hospital Universitario Dr. Peset, Valencia, - - 2014
The non-nucleoside analog reverse transcriptase inhibitor Efavirenz (EFV) has been associated with hepatic toxicity and metabolic disturbances. Although the mechanisms involved are not clear, recent evidence has pinpointed a specific mitochondrial action of EFV accompanied by ER-stress/UPR induction in human hepatic cells. The aim of this study was to further ...
Liaudanskaya Volha V University of Trento, Department of Industrial Engineering, Trento, Italy ; - - 2014
SHSY5Y human neuroblastoma cells have been encapsulated in alginate microbeads by electro hydro-dynamic jetting (EHDJ) printing. The method consists in applying a high voltage electrical field between a target substrate and a jetting needle, which is fed with a suspension of cells in a pre-polymeric solution. Depending on the conditions ...
Sidorova Tatiana N TN Departments of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, - - 2014
Rapid activation causes remodeling of atrial myocytes resembling that which occurs in experimental and human atrial fibrillation (AF). Using this cellular model, we previously observed transcriptional upregulation of proteins implicated in protein misfolding and amyloidosis. For organ-specific amyloidoses such as Alzheimer's disease, preamyloid oligomers (PAOs) are now recognized to be ...
Ghosh Suman - - 2014
Human Hsp90 isoforms are molecular chaperones that are often up-regulated in malignances and represent a primary target for Hsp90 inhibitors undergoing clinical evaluation. Hsp90α is a stress-inducible isoform of Hsp90 that plays a significant role in apoptosis and metastasis. Though Hsp90α is secreted into the extracellular space under metastatic conditions, ...
Ooi Jolene J Translational Laboratory in Genetic Medicine (TLGM), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, Immunos, Level 5, Singapore, 138648, - - 2014
Monoamine oxidases (MAO) are important components of the homeostatic machinery that maintains the levels of monoamine neurotransmitters, including dopamine, in balance. Given the imbalance in dopamine levels observed in Huntington disease (HD), the aim of this study was to examine MAO activity in a mouse striatal cell model of HD ...
Ye Zhi-Wei ZW Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, 70 President St., DD410, Charleston, SC 29425, - - 2014
Within cells, there is a narrow concentration threshold that governs whether reactive oxygen species (ROS) induce toxicity or act as second messengers. We discuss current understanding of how ROS arise, facilitate cell signaling, cause toxicities and disease related to abnormal cell differentiation and those (primarily) sulfur based pathways that provide ...
Ribas Judit - - 2014
Aerobic metabolism of mammalian cells leads to the generation of reactive oxygen species (ROS). To cope with this toxicity, evolution provided cells with effective antioxidant systems like glutathione. Current anticancer therapies focus on the cancer dependence on oncogenes and non-oncogenes. Tumours trigger mechanisms to circumvent the oncogenic stress and to ...
Ali Daoud D Department of Zoology, College of Science, King Saud University, BOX 2454, Riyadh, 11451, Saudi Arabia, - - 2014
Extensive applications of cerium oxide (CeO2) nanoparticles require a better understanding of their possible effects on human health. However, data demonstrating the effect of CeO2 nanoparticles on the human skin melanoma cell remain scanty. In the current study, we determined the mechanism through which CeO2 nanoparticles (APS <25 nm) induce toxicity ...
Kock Christian C Universität Osnabrück, Fachbereich Biologie/Chemie, AG Genetik, Barbarastr. 11, D-49076 Osnabrück, - - 2014
Yeast cell wall integrity (CWI) signaling serves as a model for the regulation of fungal cell wall synthesis and provides the basis for the development of antifungal drugs. A set of five membrane-spanning sensors (Wsc1-Wsc3, Mid2 and Mtl1) detect cell surface stress and commence the signaling pathway upon perturbations of ...
Tani Hidenori H Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan. Electronic address: - - 2014
Biosensors using live cells are analytical devices that have the advantage of being highly sensitive for their targets. Although attention has primarily focused on reporter gene assays using functional promoters, cell viability assays are still efficient. We focus on long non-coding RNAs (lncRNAs) that are involved in the molecular mechanisms ...
Cao Yan Y Hematology Center of Cyrus Tang Medical Institute, Jiangsu Institute of Hematology, Collaborative Innovation Center of Hematology, Jiangsu Key Laboratory for Stem Cell Research, Affiliated Children's Hospital, Soochow University School of Medicine, Suzhou 215123, - - 2014
Autophagy is implicated in hematopoiesis, but its role in the regulation of the hematopoietic stem and progenitor cell (HSPC) cycle remains obscure. Here, we show that autophagy is essential to maintain and regulate the cell cycle of HSPCs in a nutrient-dependent manner. The loss of autophagy via conditional deletion of ...
Liu Huailei H Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, 150001, People's Republic of China; Institute of Brain Science, Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China; Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of - - 2014
Cancer cells are highly dependent on methionine and cystine (Met-Cys) for survival and proliferation. However, the molecular mechanism is not fully clear. The present study is to investigate the effects of Met-Cys deprivation on glioma cells proliferation. The results showed that Met-Cys double deprivation had synergistic action on elevating ROS ...
Tarantini Adeline A Agence Nationale de Sécurité Sanitaire, Unité de Toxicologie des Contaminants, Bâtiment BioAgroPolis, 10B rue C, Bourgelat, BP 40608, 35306 Fougères, - - 2014
Silica (SiO2) in its nanosized form is now used in food applications although the potential risks for human health need to be evaluated in further detail. In the current study, the uptake of 15 and 55nm colloidal SiO2 NPs in the human intestinal Caco-2 cell line was investigated by transmission ...
Feng Jingyang J Department of Ophthalmology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, PR - - 2014
Aims: Age-related lipofuscin N-retinylidene-N-retinylethanolamine (A2E) accumulated in human retinal pigment epithelium (RPE) cells confers susceptibility to blue light-mediated damage, which represents one pathogenesis of age-related macular degeneration. This study investigated the expression of 2 best-characterized endoplasmic reticulum (ER) stress markers, glucose-related protein 78 (GRP78) and C/EBP homologous protein (CHOP), as ...
Arnold Markus M Department of Physiology, University of Tübingen, Tübingen, - - 2014
Background/Aims: Mitoxantrone, a cytotoxic drug used for the treatment of malignancy and multiple sclerosis, is at least in part effective by triggering apoptosis. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a type of suicidal cell death. Hallmarks of eryptosis are cell shrinkage and cell membrane scrambling with ...
Wang Z Z Institute of Bioengineering and School of Engineering and Materials Science, Queen Mary University of London, London, United - - 2014
Osteoarthritis (OA) is associated with a gradual reduction in the interstitial osmotic pressure within articular cartilage. The aim of this study was to compare the effects of sudden and gradual hypo-osmotic challenge on chondrocyte morphology and biomechanics. Bovine articular chondrocytes were exposed to a reduction in extracellular osmolality from 327 ...
Sur Arunodoy A Graduate Program in Integrative Biology, Department of Biological Sciences, Charles E. Schmidt College of Science, Florida Atlantic University, Boca Raton, FL - - 2014
The retinal pigmented epithelial (RPE) layer is one of the major ocular tissues affected by oxidative stress and is known to play an important role in the etiology of age-related macular degeneration (AMD), the major cause of blinding in the elderly. In the present study, sulindac, a nonsteroidal antiinflammatory drug ...
Ma Yuxiang Y Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing, China. Department of Internal Medicine, Beijing Chuiyangliu Hospital, - - 2014
High-affinity sodium-dependent dicarboxylate cotransporter 3 (NaDC3) is a key metabolism-regulating membrane protein responsible for transport of Krebs cycle intermediates. NaDC3 is upregulated as organs age, but knowledge regarding the underlying mechanisms by which NaDC3 modulates mammalian aging is limited. In this study, we showed that NaDC3 overexpression accelerated cellular senescence ...
Wang Xu X Basic Medicine Combined with Chinese Traditional Medicine and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, 110847, People's Republic of China, - - 2014
This study aimed to investigate the direct effects of advanced glycation end products (AGEs) on the mitochondrial structure and function of SH-SY5Y cells and the possible molecular mechanism(s) underlying mitochondria dysfunction by AGEs. SH-SY5Y cells were cultured in 400 μg/ml of AGE-bovine serum albumin (BSA) for 24 h, and changes in the ...
Zakrzewska Karolina Ewa KE Department of Hybrid Microbiosystems Engineering, Nałęcz Institute of Biocybernetics and Biomedical Engineering, PAS, Warsaw, - - 2014
The use of fluorescent markers has proven to be an attractive tool in biological imaging. However, its usefulness may be confined by the cytotoxicity of the fluorescent proteins. In this article, for the first time, we have examined an influence of the antibiotics present in culture medium on cytotoxicity of ...
Periasamy Vaiyapuri S VS Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food Science and Agriculture, Riyadh, Saudi - - 2014
The SiO2 synthesized in bulk form, adopting the conventional methods for application in food industry applications, may also contain nano-sized particles. On account of the unique physico-chemical properties, the SiO2 particulates, such as size and shape, cause metabolic toxicity in cells. Poor understanding of the molecular level nanotoxicity resulting from ...
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