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Mews Philipp P Penn Epigenetics Program Department of Cell and Developmental - - 2014
Cell growth is attuned to nutrient availability to sustain homeostatic biosynthetic processes. In unfavorable environments, cells enter a non-proliferative state termed quiescence, but rapidly return to the cell cycle once conditions support energetic needs. Changing cellular metabolite pools are proposed to directly alter the epigenome via histone acetylation. Here we ...
Orlandi Viviana Teresa VT a Department of Theoretical and Applied Sciences , University of Insubria , Varese , - - 2014
Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 ...
Wu Qiong Q School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, P. R. China. - - 2014
Effective chemotherapy drugs for cancer that would inhibit tumor growth and suppress metastasis are currently lacking. In this study, a series of arene ruthenium complexes, [(η(6)-arene)Ru(H2iip)Cl]Cl (arene = p-cymene, ; CH3C6H5, ; and C6H6, ), were synthesized and their inhibitory activity against tumor cells were evaluated. The results showed that ...
Tiwari Pooja Munnilal PM Center for NanoBiotechnology Research and Department of Biological Sciences, Alabama State University, 1627 Hall Street, Montgomery, AL 36101, - - 2014
Reduced toxicity and ease of modification make gold nanoparticles (GNPs) suitable for targeted delivery, bioimaging and theranostics by conjugating cell-penetrating peptides (CPPs). This study presents the biodistribution and enhanced intracellular uptake of GNPs functionalized with VG-21, a CPP derived from vesicular stomatitis virus glycoprotein (G). Cell penetrating efficiency of VG-21 ...
Granato Marisa M Department of Experimental Medicine, "Sapienza" University of Rome, - - 2014
Autophagy is a catabolic pathway that helps cells to survive in stressful conditions. Cells also use autophagy to clear microbiological infections but, on the other hand, microbes have learned how to manipulate the autophagic pathway for their own benefit. The experimental evidence obtained in this study suggests that the autophagic ...
Shang Fengjun F Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, - - 2014
Pseudomonas aeruginosa uses a hierarchical cell-cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilitating early medical intervention. A recently isolated cell-cell communication molecule, a ...
Durga Mohan M Centre for Nanoscience and Technology, Anna University Chennai, Ac Tech Campus, Chennai 600025, Tamil Nadu, - - 2014
Air pollution has persistently been the major cause of respiratory-related illness and death. Environmental pollutants such as diesel and petrol exhaust particles (PEPs) are the major contributors to urban air pollution. The aim of the present study was to characterize and investigate the in vitro cytotoxicity, oxidative stress, DNA damage ...
Li Yanjie - - 2014
Beyond lipid-lowering effect, statins, the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase competitively inhibitors, have recently attracted wide concern on their anticarcinogenic properties in various cancer cells. However, the molecular mechanisms by which statins inhibit cancer cell proliferation remain unclear. In this study, we investigated the effect of simvastatin on cells proliferation ...
Li Yun Y a Department of Ophthalmology and Visual Sciences, the 2nd Xiangya Hospital, Central South University, 410011, Changsha, Hunan, - - 2014
This study evaluated the protective effect of alpha lipoic acid (ALA) in human adult retinal pigment epithelium cells (ARPE-19). An RPE oxidative stress model was established in ARPE-19 cells. Cell apoptosis was detected by Annexin V/PI staining and Hoechst33342 staining. Autophagy activation was evaluated by formation of acidic vesicular organelles ...
Dhondalay Gopal Krishna GK National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College of London, London W120NN, UK; School of Science and Technology, Nottingham Trent University, Clifton Campus, Nottingham, Nottinghamshire NG11 8NS, - - 2014
The successful preparation of cells for therapy depends on the characterization of causal factors affecting cell quality. Ultra scale-down methods are used to characterize cells in terms of their response to process engineering causal factors of hydrodynamic shear stress and time. This response is in turn characterized in terms of ...
Kono Ryohei R Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University , Kimiidera 811-1, Wakayama City, Wakayama 641-0012, Japan ; Second Department of Internal Medicine, Wakayama Medical University , Kimiidera 811-1, Wakayama City, Wakayama 641-0012, - - 2014
Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects ...
Rathore Sweta S GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, Faculty of Science, National University of Singapore , - - 2014
Abstract This work evaluated the feasibility of microencapsulating Clostridium acetobutylicum ATCC 824 cells by emulsification for fermentation to produce biobutanol. The effects of selected emulsification process on viability of the vegetative cells and spores were investigated to enable the selection of appropriate form of bacterium for immobilisation. The spores were ...
Wang Shaoxiao S Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA - - 2014
The human neuronal protein α-synuclein (α-syn) has been linked by a plethora of studies as a causative factor in sporadic Parkinson's disease (PD). To speed the pace of discovery about the biology and pathobiology of α-syn, organisms such as yeast, worms, and flies have been used to investigate the mechanisms ...
Jaruszewicz Joanna J Institute of Fundamental Technological Research, Polish Academy of Sciences, 02-106 Warsaw, - - 2014
Bistable regulatory elements are important for nongenetic inheritance, increase of cell-to-cell heterogeneity allowing adaptation, and robust responses at the population level. Here, we study computationally the bistable genetic toggle switch-a small regulatory network consisting of a pair of mutual repressors-in growing and dividing bacteria. We show that as cells with ...
Chapman Lloyd A C LA Mathematical Institute, University of Oxford, Oxford, United Kingdom; Department of Computer Science, University of Oxford, Oxford, United - - 2014
The need for efficient and controlled expansion of cell populations is paramount in tissue engineering. Hollow fibre bioreactors (HFBs) have the potential to meet this need, but only with improved understanding of how operating conditions and cell seeding strategy affect cell proliferation in the bioreactor. This study is designed to ...
Nakata Keiji K Department of Emergency and Critical Care Medicine, Nippon Medical - - 2014
Mesenteric ischemia-reperfusion induces gut mucosal damage. Intestinal mucosal wounds are repaired by epithelial restitution. Although many different molecular mechanisms have been shown to affect cell metabolism under oxidative conditions, these molecular mechanisms and metabolic phenotypes are not well understood. Nuclear magnetic resonance (NMR) spectroscopic data can be used to study ...
Gu Yong Y School of Chinese Medicine, the University of Hong Kong, Hong Kong SAR, P. R. - - 2014
Site-2 protease (S2P) is a membrane-embedded protease that site-specifically cleaves intramembrane transcription factors, a necessary step for their maturation. S2P is well known to regulate cholesterol biosynthesis and endoplasmic reticulum stress in mammalian cells. In this study, we hypothesized that S2P could be responsible for the regulation of cellular oxidative ...
Ken Chuian-Fu CF Institute of Biotechnology, National Changhua University of Education, Changhua 500, Taiwan. - - 2014
Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative ...
Wang Baobei B Department of Chemical and Biochemical Engineering, Xiamen University, Xiamen, Fujian, China; Department of Human System and Environment, Arizona State University, Mesa, Arizona, United States of - - 2014
The unicellular microalga Haematococcus pluvialis has emerged as a promising biomass feedstock for the ketocarotenoid astaxanthin and neutral lipid triacylglycerol. Motile flagellates, resting palmella cells, and cysts are the major life cycle stages of H. pluvialis. Fast-growing motile cells are usually used to induce astaxanthin and triacylglycerol biosynthesis under stress ...
Noack J J German Cancer Research Centre, Program Infection and Cancer, Heidelberg, - - 2014
Glioblastomas (GBMs) are very aggressive tumors with low chemosensitivity. The DNA-alkylating agent temozolomide (TMZ) is currently the most efficient chemotoxic drug for GBM therapy; however, many patients develop resistance to TMZ. Combining TMZ with another agent could present an improved treatment option if it could overcome TMZ resistance and avoid ...
Hou Chun-Han CH Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei 100, Taiwan. - - 2014
Osteosarcoma (OS) is a relatively rare form of cancer, but OS is the most commonly diagnosed bone cancer in children and adolescents. Chemotherapy has side effects and induces drug resistance in OS. Since an effective adjuvant therapy was insufficient for treating OS, researching novel and adequate remedies is critical. Hyperthermia ...
Sarkar Ruma R Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, Calcutta, - - 2014
Heat shock proteins (HSPs) and Histone deacetylase 6 (HDAC6) are induced under oxidative stress which promotes oncogenesis. HSPs are regulated by Heat shock factor1 (HSF1). HDAC6, a class IIb deacetylase, plays an essential role in tumorigenesis and cell stress response. HSPs, HSF1 and HDAC6 are up-regulated in cancer. Present study ...
Vishavkarma Renu R Soft Condensed Matter group, Raman Research Institute, Bangalore, Karnataka, - - 2014
It is well known that substrate properties like stiffness and adhesivity influence stem cell morphology and differentiation. Recent experiments show that cell morphology influences nuclear geometry and hence gene expression profile. The mechanism by which surface properties regulate cell and nuclear properties is only beginning to be understood. Direct transmission ...
Tabone Mariangela M Department of Microbial Biotechnology, Centro Nacional de Biotecnología, CNB-CSIC, C/Darwin 3, Madrid 28049, Spain. - - 2014
Toxins of the ζ/PezT family, found in the genome of major human pathogens, phosphorylate the peptidoglycan precursor uridine diphosphate-N-acetylglucosamine (UNAG) leading to unreactive UNAG-3P. Transient over-expression of a PezT variant impairs cell wall biosynthesis and triggers autolysis in Escherichia coli. Conversely, physiological levels of ζ reversibly induce dormancy produce a ...
Foster James J Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of - - 2014
Keratoconus (KC) is a complex thinning disease of the cornea that often requires transplantation. The underlying pathogenic molecular changes in this disease are poorly understood. Earlier studies reported oxidative stress, metabolic dysfunctions and accelerated death of stromal keratocytes in keratoconus (KC) patients. Utilizing mass spectrometry we found reduced stromal extracellular ...
Herzfeld Eva E Department of Neurosurgery, Martin-Luther University of Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany. - - 2014
Nimodipine is well characterized for the management of SAH (subarachnoid hemorrhage) and has been shown to promote a better outcome and less DIND (delayed ischemic neurological deficits). In rat experiments, enhanced axonal sprouting and higher survival of motoneurons was demonstrated after cutting or crushing the facial nerve by nimodipine. These ...
Ambrosone Alfredo A Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche , Pozzuoli , - - 2014
It is generally accepted that silica (SiO2) is not toxic. But the increasing use of silica nanoparticles (SiO2NPs) in many different industrial fields has prompted the careful investigation of their toxicity in biological systems. In this report, we describe the effects elicited by SiO2NPs on animal and cell physiology. Stable ...
Pan Hongming H Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, - - 2014
Autophagy is a critical survival pathway for cancer cells under conditions of stress. Thus, induction of autophagy has emerged as a drug resistance mechanism. This study is to determine whether autophagy is activated by a novel multikinase inhibitor linifanib, thereby impairing the sensitivity of hepatocellular carcinoma (HCC) cells to this ...
Fumagalli Marzia M IFOM Foundation - FIRC Institute of Molecular Oncology Foundation, Milan, - - 2014
The DNA damage response (DDR) is activated upon DNA damage generation to promote DNA repair and inhibit cell cycle progression in the presence of a lesion. Cellular senescence is a permanent cell cycle arrest characterized by persistent DDR activation. However, some reports suggest that DDR activation is a feature only ...
Wacker Ingrid I Experimental Medicine II, Nikolaus-Fiebiger-Center, Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, - - 2014
Activin B belongs to the TGFβ family of growth factors and is upregulated in clear cell renal cell carcinoma cells by hypoxia inducible factors. Expression of Activin B is required for tumor growth in vivo and tumor cell invasion in vitro. Here we show that activation of RhoA signaling counteracts ...
Danesi Francesca F Department of Agri-Food Sciences and Technologies, University of Bologna, Piazza Goidanich, 60-47521 Cesena (FC), Italy. - - 2014
In recent years, the number of scientific papers concerning pomegranate (Punica granatum L.) and its health properties has increased greatly, and there is great potential for the use of bioactive-rich pomegranate extracts as ingredients in functional foods and nutraceuticals. To translate this potential into effective strategies it is essential to ...
Chen Yin-Quan YQ Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, Republic of - - 2014
Enterohaemorrhagic E. coli (EHEC) is a type of human pathogenic bacteria. The main virulence characteristics of EHEC include the formation of attaching and effacing lesions (A/E lesions) and the production of one or more Shiga-like toxins, which may induce human uremic complications. When EHEC infects host cells, it releases translocated ...
Gjoni Enida E Department of Medical Biotechnology and Translational Medicine, Università di Milano, LITA Segrate, Milano, - - 2014
Accumulating evidence suggests that glucolipotoxicity, arising from the combined actions of elevated glucose and free fatty acid levels, acts as a key pathogenic component in type II diabetes, contributing to β-cell dysfunction and death. Endoplasmic reticulum (ER) stress is among the molecular pathways and regulators involved in these negative effects, ...
Tang Jing J State Key Laboratory of Organ Failure Research, Key Laboratory of Transcriptomics and Proteomics, Ministry of Education of China, Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China ; Nanfang Hospital, Southern Medical University, Guangzhou 510515, - - 2014
As a core member of p38 MAPK signal transduction pathway, p38 regulated/activated kinase (PRAK) is activated by cellular stresses. However, the function of PRAK and its downstream interacting partner remain undefined. Using a yeast two-hybrid system, we identified DJ-1 as a potential PRAK interacting protein. We further verified that DJ-1 ...
Gupta Abhishekh A Laboratory of Biosystem Dynamics, Department of Signal Processing, Tampere University of Technology, FI-33101 Tampere, - - 2014
The morphological symmetry of the division process of Escherichia coli is well-known. Recent studies verified that, in optimal growth conditions, most divisions are symmetric, although there are exceptions. We investigate whether such morphological asymmetries in division introduce functional asymmetries between sister cells, and assess the robustness of the symmetry in ...
Chaves Carolina Al CA Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, - - 2014
Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules ...
Lu Wen-Li WL College of Basic Medical Science, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Shanghai 201203, - - 2014
Akebia Fructus has long been used for hepatocellular carcinoma (HCC) in China, while the molecular mechanism remains obscure. Our recent work found that Akebia trifoliate (Thunb.) Koidz seed extract (ATSE) suppressed proliferation and induced endoplasmic reticulum (ER) stress in SMMC-7721. The present study aimed to throw more light on the ...
Pathania Shailja S 1] Harvard Medical School, Boston, Massachusetts 02115, USA [2] Dana-Farber Cancer Institute, Boston, Massachusetts 02215, - - 2014
BRCA1-a breast and ovarian cancer suppressor gene-promotes genome integrity. To study the functionality of BRCA1 in the heterozygous state, we established a collection of primary human BRCA1(+/+) and BRCA1(mut/+) mammary epithelial cells and fibroblasts. Here we report that all BRCA1(mut/+) cells exhibited multiple normal BRCA1 functions, including the support of ...
Rubattu Speranza S IRCCS Neuromed, Pozzilli (Isernia), Italy; Department of Clinical and Molecular Medicine, School of Medicine and Psychology, University Sapienza of Rome, Ospedale S. Andrea, Rome, - - 2014
Abnormalities of vascular smooth muscle cells (VSMCs) contribute to development of vascular disease. Atrial natriuretic peptide (ANP) exerts important effects on VSMCs. A common ANP molecular variant (T2238C/αANP) has recently emerged as a novel vascular risk factor. We aimed at identifying effects of CC2238/αANP on viability, migration and motility in ...
Hsiao Yung-Hsuan YH Department of School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan. - - 2014
Obesity-related neurodegenerative diseases are associated with elevated saturated fatty acids (SFAs) in the brain. An increase in SFAs, especially palmitic acid (PA), triggers neuron cell apoptosis, causing cognitive function to deteriorate. In the present study, we focused on the specific mechanism by which PA triggers SH-SY5Y neuron cell apoptosis. We ...
Yen Yii-Huei YH Department of Dentistry, Ten Chan General Hospital, Chung-Li 32043, Taiwan. - - 2014
Many red algae-derived natural products are known to have anticancer effects. The biological functions of the red alga Solieria robusta from the Karachi coast (Pakistan) remain unclear. Here, we prepared a methanolic extracts of S. robusta (MESR) to examine its possible anti-oral cancer effects and the corresponding mechanism of action. ...
Chang Shaohua S Department of Surgery, Cooper University Hospital, Cooper Medical School at Rowan University, Camden, NJ - - 2014
Smith-Lemli-Opitz syndrome (SLOS) is a congenital, autosomal recessive metabolic and developmental disorder caused by mutations in the enzyme which catalyzes the reduction of 7-dehydrocholesterol (7DHC) to cholesterol. Herein we show that dermal fibroblasts obtained from SLOS children display increased basal levels of LC3B-II, the hallmark protein signifying increased autophagy. The ...
Wali Jibran A JA St Vincent's Institute, Fitzroy, Victoria, Australia; The University of Melbourne Department of Medicine, St. Vincent's Hospital, Fitzroy, Victoria, - - 2014
Loss of pancreatic beta cells is a feature of type-2 diabetes. High glucose concentrations induce endoplasmic reticulum (ER) and oxidative stress-mediated apoptosis of islet cells in vitro. ER stress, oxidative stress and high glucose concentrations may also activate the NLRP3 inflammasome leading to interleukin (IL)-1β production and caspase-1 dependent pyroptosis. ...
Fu Yao Y Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, Beijing Key Laboratory for Animal Genetic Improvement, National Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. - - 2014
The cross-talk between oocyte and somatic cells plays a crucial role in the regulation of follicular development and oocyte maturation. As a result, granulosa cell apoptosis causes follicular atresia. In this study, sheep granulosa cells were cultured under thermal stress to induce apoptosis, and melatonin (MT) was examined to evaluate ...
Prigione Alessandro A Max Delbrueck Center for Molecular Medicine (MDC), Robert-Roessle-Str. 10, D-13125 Berlin-Buch, - - 2014
Genomic aberrations induced by somatic cell reprogramming are a major drawback for future applications of this technology in regenerative medicine. A new study by Ji et al. published in Stem Cell Reports suggests a counteracting strategy based on balancing the mitochondrial/oxidative stress pathway through antioxidant supplementation.
Um Hae Young HY Department of Medical Bioscience, Dong-A University, Busan 604-714, - - 2013
Environmental stresses induce several plant pathogenic bacteria into a viable but nonculturable (VBNC) state, but the basis for VBNC is largely uncharacterized. We investigated the physiology and morphology ofthe copper-induced VBNC state in the plant pathogen Ralstonia solanacearum in liquid microcosm. Supplementation of 200 μM copper sulfate to the liquid ...
Wang Hui H Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of China, Jinan University School of Medicine, Guangzhou 510632, Guangdong Province, China ; GHM Institute of CNS Regeneration, Jinan University, Guangzhou 510632, Guangdong Province, - - 2013
The death of retinal ganglion cells is a hallmark of many optic neurodegenerative diseases such as glaucoma and retinopathy. Oxidative stress is one of the major reasons to cause the cell death. Oligomeric proanthocyanidin has many health beneficial effects including antioxidative and neuroprotective actions. Here we tested whether oligomeric proanthocyanidin ...
Wu Lili L Department of Basic Theory of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510060, Guangdong Province, - - 2013
The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) ...
van Teeseling Muriel C F - - 2013
Anammox bacteria convert ammonium and nitrite to dinitrogen gas under anaerobic conditions to obtain their energy for growth. The anammox reaction was deemed impossible until its discovery in the early 1990s. Now, anammox bacteria are recognized as major players in the global nitrogen cycle and estimated to be responsible for ...
Kriebel Katja - - 2013
Human mesenchymal stem cells (hMSCs) are multipotent by nature and are originally isolated from bone marrow. In light of a future application of hMSCs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study ...
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