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Wang Shaoxiao S Departments of Biochemistry and Molecular Biology - - 2014
Phosphatidylserine decarboxylase, which is embedded in the inner mitochondrial membrane, synthesizes phosphatidylethanolamine (PE) and, in some cells, synthesizes the majority of this important phospholipid. Normal levels of PE can decline with age in the brain. Here we used yeast and worms to test the hypothesis that low levels of PE ...
Goutzourelas N N Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, - - 2014
A grape pomace extract enhanced antioxidant mechanisms in muscle and endothelial cells both in the absence and in the presence of oxidative stress-induced agent tert-butyl hydroperoxide (tBHP). In particular, muscle (C2C12) and endothelial (EA.hy926) cells were treated with the extract at noncytotoxic concentrations for 24 h, and the oxidative stress ...
Koppenhöfer D D Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, Zweibrücken, - - 2014
Dementia is one of the big medical challenges of our time with Alzheimer's, Huntington's and Parkinson's disease among its most common forms. In year 2000, 4.5 million people were diagnosed with Alzheimer's disease in the United States. In the case of Alzheimer's disease one of many contributing factors is a ...
Rudolf Emil E Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Králové, Šimkova 870, 500 38Hradec Králové, Czech Republic. Electronic address: - - 2014
Effects of chronic exposure to supranutritional sodium selenite (Se) were investigated in colonic fibroblasts. Initially, Se did not produce any gross changes in exposed cells, however basal levels of autophagy were transiently increased and p38 activity was stimulated. From the 3rd week onwards, Se decreased cell proliferation, with corrensponding changes ...
Madadkar Haghjou Maryam M Department of Biology, Lorestan University, Khoramabad-Tehran Road (5(th) K), Iran. Electronic address: - - 2014
The effect of cold pretreatment on menadione tolerance was investigated in the cells of the marine microalga, Dunaliella viridis. In addition, the involvement of ascorbate and glutathione in the response to menadione stress was tested by treating cell suspensions with l-galactono-1,4-lactone, an ascorbate precursor, and buthionine sulfoximine, an inhibitor of ...
Tambe Dhananjay T DT Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts, United States of America. - - 2014
To quantify intercellular stresses in a cell sheet, Moussus et al. have recently proposed an approach which, under appropriate circumstances, may lead to significant simplification of the calculations required for stress recovery. Central to their approach is the assumption that the displacement fields of the substrate and the cells are ...
Shulyakova Natalya N Division of Genetics and Development Toronto Western Research Institute University Health Network, 399 Bathurst Street Toronto, Ontario, Canada M5T 2S8; Department of Physiology, University of Toronto Toronto, Ontario, Canada M5T - - 2014
Sirt3 is a mitochondrial sirtuin whose deacetylase activity regulates facets of oxidative metabolic efficiency, anti-oxidative capacity, and intra-mitochondrial signaling. In this study, we tested whether the over-expression of a human Sirt3-myc transgene in differentiated PC12 cells, a model of sympathetic catecholaminergic neurons, would affect the sensitivity of these cells to ...
Okada Minoru M Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, - - 2014
Type II collagen is a major component of cartilage. Heterozygous mutations in the type II collagen gene (COL2A1) result in a group of skeletal dysplasias known as Type II collagenopathy (COL2pathy). The understanding of COL2pathy is limited by difficulties in obtaining live chondrocytes. In the present study, we converted COL2pathy ...
Akcakaya Handan H Istanbul University, Istanbul Medical Faculty, Department of Biophysics, Istanbul, - - 2014
Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temporary arrest may ...
Wu Chenggang C Department of Microbiology & Molecular Genetics, University of Texas Health Science Center, Houston, TX, - - 2014
Sortase, a cysteine-transpeptidase conserved in Gram-positive bacteria, anchors on the cell wall many surface proteins that facilitate bacterial pathogenesis and fitness. Genetic disruption of the housekeeping sortase in several Gram-positive pathogens reported thus far attenuates virulence, but not bacterial growth. Paradoxically, we discovered that depletion of the housekeeping sortase SrtA ...
Law Courtney J CJ Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise, North Carolina Central University , Durham, North - - 2014
Abstract The underlying pathogenesis of type-II diabetes mellitus is in the dysfunction and selective loss of pancreatic islet β-cells, which ultimately leads to underproduction of endogenous insulin. Amylin, a 37-amino-acid human hormone that is cosecreted with insulin, helps regulate gastric emptying and maintain blood glucose homeostasis through improved postprandial satiety. ...
Wang Xiaoqing X Institute for Building Materials, ETH-Swiss Federal Institute of Technology Zurich, CH-8093 Zurich, Switzerland Applied Wood Materials Laboratory, EMPA-Swiss Federal Laboratories for Materials Science and Technology, CH-8600 Dübendorf, Switzerland Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, - - 2014
Bamboo is well known for its fast growth and excellent mechanical performance, but the underlying relationships between its structure and properties are only partially known. Since it lacks secondary thickening, bamboo cannot use adaptive growth in the same way as a tree would in order to modify the geometry of ...
Okamoto Mayumi M Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shoubara, Hiroshima, - - 2014
Nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay (RTD). The tRNA modifying enzymes, NSUN2 and METTL1, are mammalian orthologs of yeast Trm4 and Trm8, which are required for protecting tRNA against RTD. A simultaneous overexpression of ...
Kolhatkar Ashra A Department of Biology, Mount Allison University, Sackville, New Brunswick, Canada; 2Department of Ocean Sciences, Memorial University, St. John's, Newfoundland, - - 2014
Abstract Chemical and molecular chaperones are organic compounds that protect and stabilize proteins from damage and aggregation as a result of cellular stress. Using the dogfish (Squalus acanthias) red blood cell (RBC) as a model, we examined whether elasmobranch cells with naturally high concentrations of the chemical chaperone trimethylamine oxide ...
Steele Deborah J DJ a School of Biological Sciences , University of Essex , Colchester , - - 2014
Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ...
Lopez J H JH Department of Physics, Syracuse University, Syracuse, New York 13244, - - 2014
Experiments suggest that the migration of some cells in the three-dimensional extracellular matrix bears strong resemblance to one-dimensional cell migration. Motivated by this observation, we construct and study a minimal one-dimensional model cell made of two beads and an active spring moving along a rigid track. The active spring models ...
Ventura Clara C Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, - - 2014
It is well known the participation of oxidative stress in the induction and development of different pathologies including cancer, diabetes, neurodegeneration and respiratory disorders among others. It has been reported that oxidative stress may be induced by pesticides and it could be the cause of health alteration mediated by pollutants ...
Sano Emiko E University of Montana; University of California, - - 2014
The origin of mutations under selection has been intensively studied using the Cairns system, in which cells of an E. coli lac mutant are plated on lactose and give rise to 100 Lac(+) revertants over several days. These revertants have been attributed variously to stress-induced mutagenesis of non-growing cells or ...
Park Min M Department of Biology, York University, Toronto, - - 2014
The association between obesity and heart failure is well documented and recent studies have indicated that understanding the physiological role of autophagy will be of great significance. Cardiomyocyte apoptosis is one component of cardiac remodeling which leads to heart failure and in this study we used palmitate-treated H9c2 cells as ...
Roh Jong-Lyel JL Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of - - 2014
Adaptation to cellular stress is not a vital function of normal cells but is required of cancer cells, and as such might be a sensible target in cancer therapy. Piperlongumine is a naturally occurring small molecule selectively toxic to cancer cells. This study assesses the cytotoxicity of piperlongumine and its ...
Muñoz-Fernández Raquel R Instituto de Parasitología y Biomedicina, Consejo Superior de Investigaciones Científicas, Armilla, Granada, - - 2014
Follicular dendritic cells (FDCs) present antigens to B cells in the lymphoid follicle and inhibit B-cell apoptosis. In previous work, we obtained human FDC lines that allowed us to study the antigen phenotype and functions of these cells, finding that they expressed α-smooth muscle (SM) actin (a protein involved in ...
Mews Philipp P Penn Epigenetics Program Department of Cell and Developmental - - 2014
Cell growth is attuned to nutrient availability to sustain homeostatic biosynthetic processes. In unfavorable environments, cells enter a non-proliferative state termed quiescence, but rapidly return to the cell cycle once conditions support energetic needs. Changing cellular metabolite pools are proposed to directly alter the epigenome via histone acetylation. Here we ...
Orlandi Viviana Teresa VT a Department of Theoretical and Applied Sciences , University of Insubria , Varese , - - 2014
Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 ...
Wu Qiong Q School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, P. R. China. - - 2014
Effective chemotherapy drugs for cancer that would inhibit tumor growth and suppress metastasis are currently lacking. In this study, a series of arene ruthenium complexes, [(η(6)-arene)Ru(H2iip)Cl]Cl (arene = p-cymene, ; CH3C6H5, ; and C6H6, ), were synthesized and their inhibitory activity against tumor cells were evaluated. The results showed that ...
Tiwari Pooja Munnilal PM Center for NanoBiotechnology Research and Department of Biological Sciences, Alabama State University, 1627 Hall Street, Montgomery, AL 36101, - - 2014
Reduced toxicity and ease of modification make gold nanoparticles (GNPs) suitable for targeted delivery, bioimaging and theranostics by conjugating cell-penetrating peptides (CPPs). This study presents the biodistribution and enhanced intracellular uptake of GNPs functionalized with VG-21, a CPP derived from vesicular stomatitis virus glycoprotein (G). Cell penetrating efficiency of VG-21 ...
Granato Marisa M Department of Experimental Medicine, "Sapienza" University of Rome, - - 2014
Autophagy is a catabolic pathway that helps cells to survive in stressful conditions. Cells also use autophagy to clear microbiological infections but, on the other hand, microbes have learned how to manipulate the autophagic pathway for their own benefit. The experimental evidence obtained in this study suggests that the autophagic ...
Shang Fengjun F Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Ireland; Department of Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, - - 2014
Pseudomonas aeruginosa uses a hierarchical cell-cell communication system consisting of a number of regulatory elements to coordinate the expression of bacterial virulence genes. Sensitive detection of quorum sensing (QS) molecules has the potential for early identification of P. aeruginosa facilitating early medical intervention. A recently isolated cell-cell communication molecule, a ...
Durga Mohan M Centre for Nanoscience and Technology, Anna University Chennai, Ac Tech Campus, Chennai 600025, Tamil Nadu, - - 2014
Air pollution has persistently been the major cause of respiratory-related illness and death. Environmental pollutants such as diesel and petrol exhaust particles (PEPs) are the major contributors to urban air pollution. The aim of the present study was to characterize and investigate the in vitro cytotoxicity, oxidative stress, DNA damage ...
Li Yanjie - - 2014
Beyond lipid-lowering effect, statins, the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase competitively inhibitors, have recently attracted wide concern on their anticarcinogenic properties in various cancer cells. However, the molecular mechanisms by which statins inhibit cancer cell proliferation remain unclear. In this study, we investigated the effect of simvastatin on cells proliferation ...
Li Yun Y a Department of Ophthalmology and Visual Sciences, the 2nd Xiangya Hospital, Central South University, 410011, Changsha, Hunan, - - 2014
This study evaluated the protective effect of alpha lipoic acid (ALA) in human adult retinal pigment epithelium cells (ARPE-19). An RPE oxidative stress model was established in ARPE-19 cells. Cell apoptosis was detected by Annexin V/PI staining and Hoechst33342 staining. Autophagy activation was evaluated by formation of acidic vesicular organelles ...
Dhondalay Gopal Krishna GK National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College of London, London W120NN, UK; School of Science and Technology, Nottingham Trent University, Clifton Campus, Nottingham, Nottinghamshire NG11 8NS, - - 2014
The successful preparation of cells for therapy depends on the characterization of causal factors affecting cell quality. Ultra scale-down methods are used to characterize cells in terms of their response to process engineering causal factors of hydrodynamic shear stress and time. This response is in turn characterized in terms of ...
Kono Ryohei R Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University , Kimiidera 811-1, Wakayama City, Wakayama 641-0012, Japan ; Second Department of Internal Medicine, Wakayama Medical University , Kimiidera 811-1, Wakayama City, Wakayama 641-0012, - - 2014
Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects ...
Rathore Sweta S GEA-NUS Pharmaceutical Processing Research Laboratory, Department of Pharmacy, Faculty of Science, National University of Singapore , - - 2014
Abstract This work evaluated the feasibility of microencapsulating Clostridium acetobutylicum ATCC 824 cells by emulsification for fermentation to produce biobutanol. The effects of selected emulsification process on viability of the vegetative cells and spores were investigated to enable the selection of appropriate form of bacterium for immobilisation. The spores were ...
Chapman Lloyd A C LA Mathematical Institute, University of Oxford, Oxford, United Kingdom; Department of Computer Science, University of Oxford, Oxford, United - - 2014
The need for efficient and controlled expansion of cell populations is paramount in tissue engineering. Hollow fibre bioreactors (HFBs) have the potential to meet this need, but only with improved understanding of how operating conditions and cell seeding strategy affect cell proliferation in the bioreactor. This study is designed to ...
Nakata Keiji K Department of Emergency and Critical Care Medicine, Nippon Medical - - 2014
Mesenteric ischemia-reperfusion induces gut mucosal damage. Intestinal mucosal wounds are repaired by epithelial restitution. Although many different molecular mechanisms have been shown to affect cell metabolism under oxidative conditions, these molecular mechanisms and metabolic phenotypes are not well understood. Nuclear magnetic resonance (NMR) spectroscopic data can be used to study ...
Gu Yong Y School of Chinese Medicine, the University of Hong Kong, Hong Kong SAR, P. R. - - 2014
Site-2 protease (S2P) is a membrane-embedded protease that site-specifically cleaves intramembrane transcription factors, a necessary step for their maturation. S2P is well known to regulate cholesterol biosynthesis and endoplasmic reticulum stress in mammalian cells. In this study, we hypothesized that S2P could be responsible for the regulation of cellular oxidative ...
Ken Chuian-Fu CF Institute of Biotechnology, National Changhua University of Education, Changhua 500, Taiwan. - - 2014
Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative ...
Wang Baobei B Department of Chemical and Biochemical Engineering, Xiamen University, Xiamen, Fujian, China; Department of Human System and Environment, Arizona State University, Mesa, Arizona, United States of - - 2014
The unicellular microalga Haematococcus pluvialis has emerged as a promising biomass feedstock for the ketocarotenoid astaxanthin and neutral lipid triacylglycerol. Motile flagellates, resting palmella cells, and cysts are the major life cycle stages of H. pluvialis. Fast-growing motile cells are usually used to induce astaxanthin and triacylglycerol biosynthesis under stress ...
Noack J J German Cancer Research Centre, Program Infection and Cancer, Heidelberg, - - 2014
Glioblastomas (GBMs) are very aggressive tumors with low chemosensitivity. The DNA-alkylating agent temozolomide (TMZ) is currently the most efficient chemotoxic drug for GBM therapy; however, many patients develop resistance to TMZ. Combining TMZ with another agent could present an improved treatment option if it could overcome TMZ resistance and avoid ...
Hou Chun-Han CH Department of Orthopedic Surgery, National Taiwan University Hospital, Taipei 100, Taiwan. - - 2014
Osteosarcoma (OS) is a relatively rare form of cancer, but OS is the most commonly diagnosed bone cancer in children and adolescents. Chemotherapy has side effects and induces drug resistance in OS. Since an effective adjuvant therapy was insufficient for treating OS, researching novel and adequate remedies is critical. Hyperthermia ...
Sarkar Ruma R Department of Environmental Carcinogenesis & Toxicology, Chittaranjan National Cancer Institute, Calcutta, - - 2014
Heat shock proteins (HSPs) and Histone deacetylase 6 (HDAC6) are induced under oxidative stress which promotes oncogenesis. HSPs are regulated by Heat shock factor1 (HSF1). HDAC6, a class IIb deacetylase, plays an essential role in tumorigenesis and cell stress response. HSPs, HSF1 and HDAC6 are up-regulated in cancer. Present study ...
Vishavkarma Renu R Soft Condensed Matter group, Raman Research Institute, Bangalore, Karnataka, - - 2014
It is well known that substrate properties like stiffness and adhesivity influence stem cell morphology and differentiation. Recent experiments show that cell morphology influences nuclear geometry and hence gene expression profile. The mechanism by which surface properties regulate cell and nuclear properties is only beginning to be understood. Direct transmission ...
Tabone Mariangela M Department of Microbial Biotechnology, Centro Nacional de Biotecnología, CNB-CSIC, C/Darwin 3, Madrid 28049, Spain. - - 2014
Toxins of the ζ/PezT family, found in the genome of major human pathogens, phosphorylate the peptidoglycan precursor uridine diphosphate-N-acetylglucosamine (UNAG) leading to unreactive UNAG-3P. Transient over-expression of a PezT variant impairs cell wall biosynthesis and triggers autolysis in Escherichia coli. Conversely, physiological levels of ζ reversibly induce dormancy produce a ...
Foster James J Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of - - 2014
Keratoconus (KC) is a complex thinning disease of the cornea that often requires transplantation. The underlying pathogenic molecular changes in this disease are poorly understood. Earlier studies reported oxidative stress, metabolic dysfunctions and accelerated death of stromal keratocytes in keratoconus (KC) patients. Utilizing mass spectrometry we found reduced stromal extracellular ...
Herzfeld Eva E Department of Neurosurgery, Martin-Luther University of Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany. - - 2014
Nimodipine is well characterized for the management of SAH (subarachnoid hemorrhage) and has been shown to promote a better outcome and less DIND (delayed ischemic neurological deficits). In rat experiments, enhanced axonal sprouting and higher survival of motoneurons was demonstrated after cutting or crushing the facial nerve by nimodipine. These ...
Ambrosone Alfredo A Istituto di Cibernetica "Eduardo Caianiello", Consiglio Nazionale delle Ricerche , Pozzuoli , - - 2014
It is generally accepted that silica (SiO2) is not toxic. But the increasing use of silica nanoparticles (SiO2NPs) in many different industrial fields has prompted the careful investigation of their toxicity in biological systems. In this report, we describe the effects elicited by SiO2NPs on animal and cell physiology. Stable ...
Pan Hongming H Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, - - 2014
Autophagy is a critical survival pathway for cancer cells under conditions of stress. Thus, induction of autophagy has emerged as a drug resistance mechanism. This study is to determine whether autophagy is activated by a novel multikinase inhibitor linifanib, thereby impairing the sensitivity of hepatocellular carcinoma (HCC) cells to this ...
Fumagalli Marzia M IFOM Foundation - FIRC Institute of Molecular Oncology Foundation, Milan, - - 2014
The DNA damage response (DDR) is activated upon DNA damage generation to promote DNA repair and inhibit cell cycle progression in the presence of a lesion. Cellular senescence is a permanent cell cycle arrest characterized by persistent DDR activation. However, some reports suggest that DDR activation is a feature only ...
Um Hae Young HY Department of Medical Bioscience, Dong-A University, Busan 604-714, - - 2013
Environmental stresses induce several plant pathogenic bacteria into a viable but nonculturable (VBNC) state, but the basis for VBNC is largely uncharacterized. We investigated the physiology and morphology ofthe copper-induced VBNC state in the plant pathogen Ralstonia solanacearum in liquid microcosm. Supplementation of 200 μM copper sulfate to the liquid ...
Wang Hui H Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of China, Jinan University School of Medicine, Guangzhou 510632, Guangdong Province, China ; GHM Institute of CNS Regeneration, Jinan University, Guangzhou 510632, Guangdong Province, - - 2013
The death of retinal ganglion cells is a hallmark of many optic neurodegenerative diseases such as glaucoma and retinopathy. Oxidative stress is one of the major reasons to cause the cell death. Oligomeric proanthocyanidin has many health beneficial effects including antioxidative and neuroprotective actions. Here we tested whether oligomeric proanthocyanidin ...
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