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Solé Carme C Cell Signaling unit, Departament de Ciències Experimentals i de la Salut, Cell Signaling Research Group, Universitat Pompeu Fabra (UPF), Dr Aiguader 88, E-08003, Barcelona, - - 2014
Eukaryotic cells have developed sophisticated systems to constantly monitor changes in the extracellular environment and to orchestrate a proper cellular response. To maximize survival, cells delay cell-cycle progression in response to environmental changes. In response to extracellular insults, stress-activated protein kinases (SAPKs) modulate cell-cycle progression and gene expression. In yeast, ...
Liu Fei F 1State Key Laboratory of Mechanical Transmission,College of Mechanical Engineering,Chongqing University,Chongqing - - 2014
Mechanical properties are vital for living cells, and various models have been developed to study the mechanical behavior of cells. However, there is debate regarding whether a cell behaves more similarly to a "cortical shell - liquid core" structure (membrane-like) or a homogeneous solid (cytoskeleton-like) when experiencing stress by mechanical ...
Savion Naphtali N Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel Aviv University, Sheba Medical Center, Tel Hashomer 52621, Israel. Electronic address: - - 2014
Oxidative stress and/or low cellular glutathione are associated with development and progression of neurodegenerative diseases. We have shown that S-allylmercapto-N-acetylcysteine (ASSNAC) up-regulates the level of glutathione and phase II detoxifying enzymes in cultured vascular endothelial cells. The present study demonstrates that exposure of nerve cell lines to ASSNAC significantly increases ...
Banerjee Priyanjalee P Department of Biochemistry, Institute of Postgraduate Medical Education and Research, 244, AJC Bose Road, Kolkata, 700020, - - 2014
The increased accumulation of iron in the brain in Alzheimer's disease (AD) is well documented, and excess iron is strongly implicated in the pathogenesis of the disease. The adverse effects of accumulated iron in AD brain may include the oxidative stress, altered amyloid beta-metabolism and the augmented toxicity of metal-bound ...
Lin Chia-Yu CY Department of Health and Nutrition Biotechnology, Asia University , Taichung, Taiwan - - 2014
Abstract Oxidative stress has been implicated in the etiology of pancreatic β-cell dysfunction and diabetes. Studies have shown that chlorella could be important in health promotion or disease prevention through its antioxidant capacity. However, whether chlorella has a cytoprotective effect in pancreatic β-cells remains to be elucidated. We investigated the ...
Hariharan V V Department of Biomedical Engineering, Columbia University, New York, - - 2014
The majority of patients diagnosed with arrhythmogenic right ventricular cardiomyopathy (ARVC) have mutations in genes encoding desmosomal proteins, raising the possibility that abnormal intercellular adhesion plays an important role in disease pathogenesis. We characterize cell mechanical properties and molecular responses to oscillatory shear stress in cardiac myocytes expressing mutant forms ...
Xia Meihui M Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. - - 2014
The mechanisms underlying cisplatin resistance in tumors are not fully understood. Previous studies have reported that cellular resistance to oxidative stress is accompanied by resistance to cisplatin. However, the relationship between the resistance to oxidative stress and cisplatin drug resistance in human ovarian cancer cells (HOCCs) is not clear. Here, ...
Kabiraj Parijat - - 2014
Nitrosative stress mediated S-nitrosylation (SNO) of protein disulfide isomerase (PDI), a housekeeping oxidoreductase, has been implicated in the pathogenesis of sporadic Parkinson's (PD) and Alzheimer's (AD) diseases. Previous cell line studies have indicated that SNO-PDI formation provokes synphilin-1 aggregation, the minor Parkinsonian biomarker protein. Yet no work exists investigating whether ...
Wang W W Institute of Urology, Huashan Hospital, Fudan University, Shanghai, - - 2014
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role of PPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were ...
Song Kibbeum K Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk, 561-156, Republic of - - 2014
Alcoholism, which refers to the excessive consumption of alcohol, has deleterious effects on personal and social health worldwide. Oxidative stress evoked by ethanol plays an important role in the pathogenesis of neurodegenerative diseases. Rutin is a bioflavonoid that has been demonstrated to scavenge superoxide radicals. However, the effects of rutin ...
Wang W W Institute of Urology, Huashan Hospital, Fudan University, Shanghai, - - 2014
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role of PPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were ...
Gorelova Olga O Department of Bioengineering Faculty of Biology, Moscow State University, GSP-1, 119234, Moscow, - - 2014
A quantitative micromorphometric study of the cell compartment rearrangements was performed in a symbiotic chlorophyte Desmodesmus sp. 3Dp86E-1 grown on nitrogen (N) replete or N-free medium under 480 μmol PAR quanta m(-2) s(-1). The changes in the chloroplast, intraplastidial, and cytoplasmic inclusions induced by high light (HL) and N starvation were similar ...
Dasmahapatra Girija G Internal Medicine, Virginia Commonwealth - - 2014
Interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor Carfilzomib (CFZ) were examined in non-Hodgkin's lymphoma models, including diffuse large B-cell (DLBCL), mantle cell (MCL) and double-hit lymphoma cells. Marked in vitro synergism was observed in multiple cell types associated with activation of cellular stress pathways (e.g., ...
Ghandhi Shanaz A - - 2014
Radiation induced bystander effects are an important component of the overall response of cells to irradiation and are associated with human health risks. The mechanism responsible includes intra-cellular and inter-cellular signaling by which the bystander response is propagated. However, details of the signaling mechanism are not well defined. We measured ...
Zhong Bin B Department of Bioscience, College of life science, Nanchang University, Nanchang 330031, - - 2014
Heavy metal ion is one of the critical environmental pollutants accumulated in living organisms and causes toxic or carcinogenic effects once passed threshold levels. As an important member of Hsp70 (heat shock protein 70) family, the 78-kDa glucose-regulated protein (GRP78) can enhance cell survival rates remarkably under thermal stress. Recent ...
McGoldrick Christopher A - - 2014
Cancer cell esterases are often overexpressed and can have chiral specificities different from that of the corresponding normal cells and can, therefore, be useful targets for activating chemotherapeutic prodrug esters. Prodrug esters are inactive compounds that can be preferentially activated by esterase enzymes. Moreover, cancer cells often exhibit a high ...
Urano Daisuke D Department of Biology at the University of North Carolina, Chapel Hill, NC, 27599-3280, - - 2014
The plant G-protein network, comprising Gα, Gβ, and Gγ core subunits, regulates development, senses sugar, and mediates biotic and abiotic stress responses. Here, we report G-protein signalling in the salt stress response using two crop models, rice and maize. Loss-of-function mutations in the corresponding genes encoding the Gα subunit attenuate ...
Blum Barak B Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, United - - 2014
Dysfunction or death of pancreatic β cells underlies both types of diabetes. This functional decline begins with β cell stress and de-differentiation. Current drugs for T2D lower blood glucose levels, but they do not directly alleviate β cell stress nor prevent, let alone reverse, β cell de-differentiation. We show here ...
Giri Sib Sankar SS Dept. of Biotechnology, Periyar Maniammai University, Thanjavur-613403, Tamil Nadu, India. Electronic address: - - 2014
To understand the function of HSP70 of Labeo rohita (LrHSP70) in cellular protection, LrHSP70 ORF cDNA was inserted into the plasmid of pET-32a(+) or pEGFP-L1. Then, the recombinant plasmids were transformed or transfected into E. coli cells, mouse myeloma cells (MPC-11) or fish hepatoma cells (PLHC-1). Western blot results revealed ...
Zhou Shi-Wei SW Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. - - 2014
Simple hepatic steatosis is the early stage of non‑alcoholic fatty liver disease and is recognized as a benign process. Previous studies show that glucagon‑like peptide‑1 has great potential in improving hepatic steatosis. Recent data have revealed that inhibiting autophagy exacerbates lipid accumulation in hepatocytes. Therefore, the present study aimed to ...
Salleron Lisa L Department of Human Protein Sciences, Faculty of Medicine, University of Geneva. Electronic address: - - 2014
Deoxyribose-phosphate aldolase (EC 4.1.2.4), which converts 2-deoxy-D-ribose-5-phosphate into glyceraldehyde-3-phosphate and acetaldehyde, belongs to the core metabolism of living organisms. It was previously shown that human cells harbor deoxyribose phosphate aldolase activity but the protein responsible of this activity has never been formally identified. This study provides the first experimental evidence ...
Kanan Y Y Department of Cell Biology, University of Oklahoma Health Sciences Center, BMSB 781, 940 Stanton L. Young Blvd., Oklahoma City, OK, 73104, - - 2014
Docosahexaenoic acid (DHA), an omega-3 fatty acid family member, is obtained by diet or synthesized from dietary essential omega-3 linolenic acid and delivered systemically to the choriocapillaris, from where it is taken up by the retinal pigment epithelium (RPE). DHA is then transported to the inner segments of photoreceptors, where ...
Sieroslawska Anna A Department of Physiology and Ecotoxicology, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708, Lublin, - - 2014
Cylindrospermopsin (CYN) is a cyanotoxin detected in water reservoirs worldwide. The toxin is a potent protein synthesis inhibitor capable of adversely influencing a wide range of cell functions. While data on the prooxidative potency of CYN are inconsistent, genotoxic effects towards certain mammalian cell types have been described. However, such ...
He Yuan Y Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, 167 Fang Dong Street, Xi'an, Shannxi, 710038, - - 2014
PURPOSE. We have previously shown that aging RPE cells are highly susceptible to oxidative stress, which impairs their mitochondrial function. This study investigates whether the neuroprotective PEDF can prevent mitochondrial pathology in aging RPE cells exposed to oxidative stress. METHODS. Human RPE cells were isolated from 50-75-year-old eyes and cultured ...
Lynge Martin E ME iNANO, Aarhus University, Gustav Wieds Vej 14, Aarhus, 8000, - - 2014
Implantable devices equipped with coatings which have the ability to carry and deliver active compounds are of great interest. We report the assembly of liposome-containing poly(dopamine) films, and their interaction with adhering cells. The liposome composition is varied by adding lipophilic dopamine-conjugates and charged lipids. The cell mean fluorescence (CMF) ...
Kim So-Jung SJ Department of Biochemistry, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea; MD-PhD program, Pusan National University School of Medicine, Yangsan 626-870, Republic of - - 2014
In the present study, we investigated whether celecoxib could induce the expression of NKG2D ligands in clonogenic colon cancer cells, and increase their susceptibility to NK cell-mediated cell death. Celecoxib and its non-coxib analogue, 2,5-dimethyl celecoxib, induced ULBP-1 and DR5 in both COX-2 negative HCT-15 cells and COX-2 positive HT-29 ...
Daubney James J School of Science and Technology, Nottingham Trent University, Nottingham, United - - 2014
Whilst mitotic rat embryonic cardiomyoblast-derived H9c2 cells have been widely used as a model system to study the protective mechanisms associated with flavonoids, they are not fully differentiated cardiac cells. Hence, the aim of this study was to investigate the cardioprotective and cardiotoxic actions of quercetin and two of its ...
Merabova Nana N Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, 3500 North Broad Street, 7th Floor, Philadelphia, PA, - - 2014
Autophagy is an evolutionarily conserved, selective degradation pathway of cellular components that is important for cell homeostasis under healthy and pathologic conditions. Here we demonstrate that an increase in the level of BAG3 results in stimulation of autophagy in glioblastoma cells. BAG3 is a member of a co-chaperone family of ...
Gall Jonathan M JM Renal Section - - 2014
Proximal tubule (PT) cells are critical targets of acute ischemic injury. Elimination of the mitochondrial fusion protein mitofusin 2 (Mfn2) sensitizes PT cells to apoptosis in vitro. However, the role of PT Mfn2 in ischemic AKI in vivo is unknown. To test its role, we evaluated the effects of conditional ...
Wang Shaoxiao S Departments of Biochemistry and Molecular Biology - - 2014
Phosphatidylserine decarboxylase, which is embedded in the inner mitochondrial membrane, synthesizes phosphatidylethanolamine (PE) and, in some cells, synthesizes the majority of this important phospholipid. Normal levels of PE can decline with age in the brain. Here we used yeast and worms to test the hypothesis that low levels of PE ...
Goutzourelas N N Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, - - 2014
A grape pomace extract enhanced antioxidant mechanisms in muscle and endothelial cells both in the absence and in the presence of oxidative stress-induced agent tert-butyl hydroperoxide (tBHP). In particular, muscle (C2C12) and endothelial (EA.hy926) cells were treated with the extract at noncytotoxic concentrations for 24 h, and the oxidative stress ...
Koppenhöfer D D Department of Informatics and Microsystem Technology, University of Applied Sciences, Kaiserslautern, Zweibrücken, - - 2014
Dementia is one of the big medical challenges of our time with Alzheimer's, Huntington's and Parkinson's disease among its most common forms. In year 2000, 4.5 million people were diagnosed with Alzheimer's disease in the United States. In the case of Alzheimer's disease one of many contributing factors is a ...
Rudolf Emil E Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Králové, Šimkova 870, 500 38Hradec Králové, Czech Republic. Electronic address: - - 2014
Effects of chronic exposure to supranutritional sodium selenite (Se) were investigated in colonic fibroblasts. Initially, Se did not produce any gross changes in exposed cells, however basal levels of autophagy were transiently increased and p38 activity was stimulated. From the 3rd week onwards, Se decreased cell proliferation, with corrensponding changes ...
Madadkar Haghjou Maryam M Department of Biology, Lorestan University, Khoramabad-Tehran Road (5(th) K), Iran. Electronic address: - - 2014
The effect of cold pretreatment on menadione tolerance was investigated in the cells of the marine microalga, Dunaliella viridis. In addition, the involvement of ascorbate and glutathione in the response to menadione stress was tested by treating cell suspensions with l-galactono-1,4-lactone, an ascorbate precursor, and buthionine sulfoximine, an inhibitor of ...
Tambe Dhananjay T DT Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts, United States of America. - - 2014
To quantify intercellular stresses in a cell sheet, Moussus et al. have recently proposed an approach which, under appropriate circumstances, may lead to significant simplification of the calculations required for stress recovery. Central to their approach is the assumption that the displacement fields of the substrate and the cells are ...
Shulyakova Natalya N Division of Genetics and Development Toronto Western Research Institute University Health Network, 399 Bathurst Street Toronto, Ontario, Canada M5T 2S8; Department of Physiology, University of Toronto Toronto, Ontario, Canada M5T - - 2014
Sirt3 is a mitochondrial sirtuin whose deacetylase activity regulates facets of oxidative metabolic efficiency, anti-oxidative capacity, and intra-mitochondrial signaling. In this study, we tested whether the over-expression of a human Sirt3-myc transgene in differentiated PC12 cells, a model of sympathetic catecholaminergic neurons, would affect the sensitivity of these cells to ...
Okada Minoru M Cell Induction and Regulation Field, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, - - 2014
Type II collagen is a major component of cartilage. Heterozygous mutations in the type II collagen gene (COL2A1) result in a group of skeletal dysplasias known as Type II collagenopathy (COL2pathy). The understanding of COL2pathy is limited by difficulties in obtaining live chondrocytes. In the present study, we converted COL2pathy ...
Akcakaya Handan H Istanbul University, Istanbul Medical Faculty, Department of Biophysics, Istanbul, - - 2014
Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temporary arrest may ...
Wu Chenggang C Department of Microbiology & Molecular Genetics, University of Texas Health Science Center, Houston, TX, - - 2014
Sortase, a cysteine-transpeptidase conserved in Gram-positive bacteria, anchors on the cell wall many surface proteins that facilitate bacterial pathogenesis and fitness. Genetic disruption of the housekeeping sortase in several Gram-positive pathogens reported thus far attenuates virulence, but not bacterial growth. Paradoxically, we discovered that depletion of the housekeeping sortase SrtA ...
Law Courtney J CJ Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise, North Carolina Central University , Durham, North - - 2014
Abstract The underlying pathogenesis of type-II diabetes mellitus is in the dysfunction and selective loss of pancreatic islet β-cells, which ultimately leads to underproduction of endogenous insulin. Amylin, a 37-amino-acid human hormone that is cosecreted with insulin, helps regulate gastric emptying and maintain blood glucose homeostasis through improved postprandial satiety. ...
Wang Xiaoqing X Institute for Building Materials, ETH-Swiss Federal Institute of Technology Zurich, CH-8093 Zurich, Switzerland Applied Wood Materials Laboratory, EMPA-Swiss Federal Laboratories for Materials Science and Technology, CH-8600 Dübendorf, Switzerland Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, - - 2014
Bamboo is well known for its fast growth and excellent mechanical performance, but the underlying relationships between its structure and properties are only partially known. Since it lacks secondary thickening, bamboo cannot use adaptive growth in the same way as a tree would in order to modify the geometry of ...
Okamoto Mayumi M Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shoubara, Hiroshima, - - 2014
Nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay (RTD). The tRNA modifying enzymes, NSUN2 and METTL1, are mammalian orthologs of yeast Trm4 and Trm8, which are required for protecting tRNA against RTD. A simultaneous overexpression of ...
Kolhatkar Ashra A Department of Biology, Mount Allison University, Sackville, New Brunswick, Canada; 2Department of Ocean Sciences, Memorial University, St. John's, Newfoundland, - - 2014
Abstract Chemical and molecular chaperones are organic compounds that protect and stabilize proteins from damage and aggregation as a result of cellular stress. Using the dogfish (Squalus acanthias) red blood cell (RBC) as a model, we examined whether elasmobranch cells with naturally high concentrations of the chemical chaperone trimethylamine oxide ...
Steele Deborah J DJ a School of Biological Sciences , University of Essex , Colchester , - - 2014
Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ...
Lopez J H JH Department of Physics, Syracuse University, Syracuse, New York 13244, - - 2014
Experiments suggest that the migration of some cells in the three-dimensional extracellular matrix bears strong resemblance to one-dimensional cell migration. Motivated by this observation, we construct and study a minimal one-dimensional model cell made of two beads and an active spring moving along a rigid track. The active spring models ...
Ventura Clara C Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, - - 2014
It is well known the participation of oxidative stress in the induction and development of different pathologies including cancer, diabetes, neurodegeneration and respiratory disorders among others. It has been reported that oxidative stress may be induced by pesticides and it could be the cause of health alteration mediated by pollutants ...
Sano Emiko E University of Montana; University of California, - - 2014
The origin of mutations under selection has been intensively studied using the Cairns system, in which cells of an E. coli lac mutant are plated on lactose and give rise to 100 Lac(+) revertants over several days. These revertants have been attributed variously to stress-induced mutagenesis of non-growing cells or ...
Park Min M Department of Biology, York University, Toronto, - - 2014
The association between obesity and heart failure is well documented and recent studies have indicated that understanding the physiological role of autophagy will be of great significance. Cardiomyocyte apoptosis is one component of cardiac remodeling which leads to heart failure and in this study we used palmitate-treated H9c2 cells as ...
Roh Jong-Lyel JL Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of - - 2014
Adaptation to cellular stress is not a vital function of normal cells but is required of cancer cells, and as such might be a sensible target in cancer therapy. Piperlongumine is a naturally occurring small molecule selectively toxic to cancer cells. This study assesses the cytotoxicity of piperlongumine and its ...
Muñoz-Fernández Raquel R Instituto de Parasitología y Biomedicina, Consejo Superior de Investigaciones Científicas, Armilla, Granada, - - 2014
Follicular dendritic cells (FDCs) present antigens to B cells in the lymphoid follicle and inhibit B-cell apoptosis. In previous work, we obtained human FDC lines that allowed us to study the antigen phenotype and functions of these cells, finding that they expressed α-smooth muscle (SM) actin (a protein involved in ...
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