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Zhao Baozhong B University of Chicago, United - - 2014
Skin cancer is the most common cancer in the United States and is mainly caused by environmental UV radiation. Reducing skin cancer incidence is becoming an urgent issue. The stress-inducible protein Sestrin2 (Sesn2) plays an important role in maintaining redox and metabolic homeostasis and their related pathologies. However, the role ...
Lei Yuan Y Research Centre, Eye and ENT hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, - - 2014
Purpose:To investigate effects of aging on endothelial nitric oxide synthase (eNOS) expression and signaling of angular aqueous plexus (AAP, functional equivalent to human Schlemm's canal) cells subjected to shear stress. Method: AAP cells were isolated differentially from porcine outflow tissues using puromycin selection. Cell aging was induced by culturing cells ...
Freije Ana A Cell Cycle, Stem Cell Fate and Cancer Laboratory, Fundación Instituto de Investigación Marqués de Valdecilla (IDIVAL), Santander 39011, - - 2014
Tumor suppressor p53 is a major cellular guardian of genome integrity, and its inactivation is the most frequent genetic alteration in cancer, rising up to 80% in squamous cell carcinoma (SCC). By adapting the small hairpin RNA (shRNA) technology, we inactivated endogenous p53 in primary epithelial cells from the epidermis of human ...
Luo Chun-Shan - - 2014
Investigation of how diatoms cope with the rapid fluctuations in iron bioavailability in marine environments may facilitate a better understanding of the mechanisms underlying their ecological success, in particular their ability to proliferate rapidly during favorable conditions. In this study, using in vivo biochemical markers and whole-cell iTRAQ-based proteomics analysis, ...
Sui Xinbing X Department of Medical Oncology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. - - 2014
The tumor suppressor p53 is widely known for its ability to induce cell cycle arrest or cell death, therefore preventing neoplastic progression. Previous studies have demonstrated novel roles for p53 in the regulation of autophagy and senescence. p53 can not only exert cell cycle‑arresting and senescence‑promoting or suppressing functions, but ...
Hong Shaocheng S State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, - - 2014
The aim of this study is to understand the underlying mechanisms regulating the adverse effect of diarrhea caused by epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs). We comparatively examined the effects of two EGFR-TKIs, gefitinib and icotinib, on intestinal epithelial cells (IEC-6). Cell proliferation was measured using MTT analysis. ...
Mukhopadhyay Debabrata D Department of Biochemistry and Molecular Biology, Mayo Clinic - - 2014
Purpose:Pancreatic cancer (PC) frequently causes diabetes. We recently proposed adrenomedullin (AM) as a candidate mediator of pancreatic β-cell dysfunction in PC. How PC-derived AM reaches β-cells remote from the cancer to induce β-cell dysfunction is unknown. We tested a novel hypothesis that PC sheds AM-containing exosomes into circulation which are ...
Nistri Silvia - - 2014
Abstract Reperfusion injury after oxygen starvation is a key pathogenic step of ischemic diseases. It mainly consists in oxidative stress, related to mitochondrial derangement and enhanced generation of reactive oxygen species (ROS), mainly superoxide anion (O2), and of peroxynitrite by cells exposed to hypoxia. This in vitro study evaluates whether ...
Sohm Bénédicte B Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR CNRS 7360, Université de Lorraine, F-57070, Metz, - - 2014
Large scale production and incorporation of titanium dioxide nanoparticles (NP-TiO2 ) in consumer products leads to their potential release into the environment and raises the question of their toxicity. The bactericidal mechanism of titanium dioxide nanoparticles (NP-TiO2 ) under UV light is known to involve oxidative stress due to the ...
Chibazakura Taku T a Department of Bioscience , Tokyo University of Agriculture , Tokyo , - - 2014
5-aminolevulinic acid (5-ALA) is contained in all organisms and a starting substrate for heme biosynthesis. Since administration of 5-ALA specifically leads cancer cells to accumulate protoporphyrin IX (PpIX), a potent photosensitizer, we tested if 5-ALA also serves as a thermosensitizer. 5-ALA enhanced heat-induced cell death of cancer cell lines such ...
Lim Chang-Jin CJ Department of Biochemistry, College of Natural Sciences, Kangwon National University, 192-1 Hyoja-2-dong, Chuncheon, 200-701, Korea, - - 2014
Hos3 is involved in cellular growth under osmotic stress in Schizosaccharomyces pombe. The recombinant plasmid pYFHos3 harboring the structural gene encoding Hos3 was constructed. The S. pombe cells harboring pYFHos3 contained the increased hos3 (+) mRNA content and exhibited an enhanced growth in high osmotic conditions, such as 1.5 M KCl ...
Nurhayati Retno Wahyu RW Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, 560-8531, - - 2014
Reactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK ...
Cui Guozhen G *State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China †Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Guangdong, China ‡Stem Cell & Regenerative Medicine Consortium, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China §Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, - - 2014
Baicalein is one of the major flavonoids found in the root of Scutellaria baicalensis Georgi. Previous studies suggest that baicalein displays protective effect on experimental cardiac models in vitro and in vivo. However, the mode of action remains unclear. Here we showed that baicalein conferred cardioprotective effect against oxidative stress-induced ...
Wilmes Anja A Division of Physiology, Department of Physiology and Medical Physics, Medical University of Innsbruck, Innsbruck 6020, Austria. Electronic address: - - 2014
Cisplatin is one of the most widely used chemotherapeutic agents for the treatment of solid tumours. The major dose-limiting factor is nephrotoxicity, in particular in the proximal tubule. Here, we use an integrated omics approach, including transcriptomics, proteomics and metabolomics coupled to biokinetics to identify cell stress response pathways induced ...
Lee Mei-Chong Wendy MC Biomolecular Engineering Department, University of California, Santa Cruz, CA - - 2014
The acute cellular response to stress generates a subpopulation of reversibly stress-tolerant cells under conditions that are lethal to the majority of the population. Stress tolerance is attributed to heterogeneity of gene expression within the population to ensure survival of a minority. We performed whole transcriptome sequencing analyses of metastatic ...
Pomiès Pascal P INSERM U-1046, University Montpellier I, University Montpellier II, Montpellier, - - 2014
The mechanisms leading to skeletal limb muscle dysfunction in chronic obstructive pulmonary disease (COPD) have not been fully elucidated. Exhausted muscle regenerative capacity of satellite cells has been evocated, but the capacity of satellite cells to proliferate and differentiate properly remains unknown. Our objectives were to compare the characteristics of ...
Wu Ryan T Y RT University of Maryland, United - - 2014
Oxidative stress and persistent DNA damage response contribute to cellular senescence, a degeneration process critically involving ataxia telangiectasia mutated (ATM) and p53. Selenoprotein H (SelH), a nuclear selenoprotein, is proposed to carry redox and transactivation domains. To determine the role of SelH in genome maintenance, shRNA knockdown was employed in ...
Martin Shaun S Northern Institute for Cancer Research, Medical School, Newcastle University, NE2 4HH, - - 2014
Glucose-regulated protein 78 (GRP78) is a stress sensor which interacts with unfolded protein response (UPR) activators in the endoplasmic reticulum (ER). The aim of this study was to test the hypothesis that GRP78 has distinct functional roles in mediating the effects of ER stress in neuroblastoma compared to other neuroectodermal ...
Ooi Theng Choon TC Biomedical Science Programme, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, - - 2014
The aim of this study is to investigate the ability of zinc carnosine to protect the human lymphoblastoid (WIL2-NS) cell line from hydrogen peroxide-induced DNA damage. Cells were cultured with medium containing zinc carnosine at the concentrations of 0.4, 4, 16 and 32 μM for 9 days prior to treatment with 30 μM ...
Kim Si Hyoung SH Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University, Chuncheon, Republic of - - 2014
In this study, we evaluated the effect of the hsp70 inhibitor VER155008 on survival of anaplastic thyroid carcinoma (ATC) cells. In ATC cells, VER155008 increased the percentages of dead cells and vacuolated cells. VER155008 did not lead to the cleavage of caspase-3 protein regardless of pretreatment with z-VAD-fmk. VER155008 increased ...
Ailawadi Shaina S Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH - - 2014
The heart is a very complex conglomeration of organized interactions between various different cell types that all aid in facilitating myocardial function through contractility, sufficient perfusion, and cell-to-cell reception. In order to make sure all features of the heart work effectively, it is imperative to have a well-controlled communication system ...
Cheng Yu-Chi YC Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, - - 2014
Endoplasmic reticulum stress occurs in a variety of patho-physiological mechanisms and there has been great interest in managing this pathway for the treatment of clinical diseases. Autophagy is closely interconnected with endoplasmic reticulum stress to counteract the possible injurious effects related with the impairment of protein folding. Studies have shown ...
Roh Jong-Lyel JL Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of - - 2014
Adaptation to cellular stress is not a vital function of normal cells but is required of cancer cells, and as such might be a sensible target in cancer therapy. Piperlongumine is a naturally occurring small molecule selectively toxic to cancer cells. This study assesses the cytotoxicity of piperlongumine and its ...
Lee G T GT Department of Obstetrics and Gynecology, University of Kansas Medical Center, Kansas City, KS, - - 2014
To assess the concentrations of inositol and sorbitol, and determine the expression of related osmolyte factors [nuclear factor of activated T cells 5, also known as tonicity responsive binding protein (NFAT5/TonEBP); sodium myo-inositol transporter (SLC5A3); and aldose reductase] in placentas of pre-eclamptic (PE) patients and trophoblast BeWo cells subjected to ...
Bonakdar Navid N Department of Physics, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, - - 2014
Plectin is the prototype of an intermediate filament (IF)-based cytolinker protein. It affects cells mechanically by interlinking and anchoring cytoskeletal filaments and acts as scaffolding and docking platform for signaling proteins to control cytoskeleton dynamics. The most common disease caused by mutations in the human plectin gene, epidermolysis bullosa simplex ...
Cai Meng M State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University , Guangzhou , P.R. - - 2014
Abstract Purpose: To investigate the effects of the mitochondria-targeted peptide, SS31, on cultured human lens epithelial cells (HLECs) under conditions of oxidative stress. Methods: Optimal concentrations of SS31 were determined by MTT assay. HLEB-3 cells were first treated with SS31 for 2 h and then with 500 µM t-BHP for 6 h. Cell ...
Campelo Riward R Instituto de Estudios Avanzados, Centro de Biotecnologia, MppEUCT, Caracas, - - 2014
In its canonical role the reverse transcriptase telomerase recovers the telomeric repeats that are lost during the DNA replication. In mammalian cells other locations and activities have been recently described for the telomerase protein subunit TERT. In the present work using biochemistry, molecular biology and electron microscopy techniques, we have ...
Rayen Ine I School for Mental Health and Neuroscience, Department of Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, - - 2014
During pregnancy and postpartum period, 20 % of women are affected by depression, which is a growing health concern. Selective serotonin reuptake inhibitor (SSRI) medications are popular treatments for maternal depression; however, the effect of maternal depression and perinatal SSRI exposure on offspring's neural development needs further investigation. This study aims ...
Zheng Yong Y Department of Anatomy and Embryology, Wuhan University School of Medicine, Wuhan, Hubei 430071, P.R. - - 2014
Following a limited number of cell divisions, mesenchymal stem cells (MSCs) undergo senescence, and these senescent cells maintain metabolic modification and remain viable for long periods. Autophagy, an intracellular bulk degradation process, provides a survival effect for cells under stress. In this study, the effect of autophagy on senescent MSCs ...
Zhang Qiu-Xiang QX State Key Laboratory of Food Science and Technology, School of Food Science and Technology,Jiangnan University,1800 Lihu Avenue, Wuxi, Jiangsu - - 2014
The relationship between hydrophobicity and the protective effect of whey protein hydrolysates (WPHs) against oxidative stress was studied. Whey protein was first hydrolysed by pepsin and trypsin to obtain WPHs. After absorbed by macroporous adsorption resin DA201-C, three fractions named as M20, M40, and M60 were eluted by various concentrations ...
Sun Hongli H Shaanxi Institute of Pediatric Diseases, The Affiliated Children Hospital of Xi'an Jiaotong University College of Medicine, Shaanxi 86-710003, PR - - 2014
To clarify the alterations of proliferation and differentiation of hippocampal cells in prenatally stressed rats. We investigated the impact of prenatal restraint stress on the hipocampal cell proliferation in the progeny with 5-bromo-2'-deoxyuridine (BrdU), which is a marker of proliferating cells and their progeny. In addition, we observed the differentiation ...
Yu Chao C The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, - - 2014
Manganese oxide nanoparticles (MnO NPs) have been regarded as a new class of T1-positive contrast agents. The cytotoxicity of silica coated MnO NPs (MnO@SiO2 NPs) was investigated in human cervical carcinoma cells (HeLa) and mouse fibroblast cells (L929). The changes of cell viability, cell morphology, cellular oxidative stress, mitochondrial membrane ...
Sun Shan S Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, - - 2014
Although cells often can tolerate oxidative environments, abnormal oxidative stress has been identified in inflammation, cardiovascular and neurodegenerative diseases, and aging. The impact of oxidative stress on the cellular biomechanics is poorly understood, however. In this study, we used C2C12 myoblasts to investigate the effect of oxidative stress, mimicked by ...
Parplys Ann C AC Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United - - 2014
RAD51-associated protein 1 (RAD51AP1) is critical for homologous recombination (HR) by interacting with and stimulating the activities of the RAD51 and DMC1 recombinases. In human somatic cells, knockdown of RAD51AP1 results in increased sensitivity to DNA damaging agents and to impaired HR, but the formation of DNA damage-induced RAD51 foci ...
Bradley Eric E Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, 1120 15th Street, Augusta, GA, 30912, - - 2014
Stress adaptation effect provides cell protection against ischemia induced apoptosis. Whether this mechanism prevents other types of cell death in stroke is not well studied. This is an important question for regenerative medicine to treat stroke since other types of cell death such as necrosis are also prominent in the ...
Kim Eun-Mee EM Department of Emergency Medical Technology, Korea Nazarene University, Cheonan 331-718, South - - 2014
We have previously demonstrated that matrix metalloprotease-3 (MMP-3) can act inside the cell to trigger apoptosis in response to various cell stresses in dopaminergic neuronal cells. However, the mechanism by which MMP-3 activity leads to caspase-3 activation in apoptotic signaling was not known. In the present study, we found that ...
Kawase Tomoyuki T Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan; Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan. Electronic address: - - 2014
For successful cell transplantation therapy, the quality of cells must be strictly controlled. Unfortunately, to exclude inappropriate cells that possess structurally abnormal chromosomes, currently only karyotyping functions as an assessment. Unfortunately, this methodology is time-consuming and only effective for metaphasic cells. To develop a more efficient, inclusive and sensitive methodology, ...
Tran Kim M - - 2014
Endoplasmic reticulum (ER) stress plays an important role in the decline in pancreatic β cell function and mass observed in type 2 diabetes. Here, we developed a novel β cell-based high-throughput screening assay to identify small molecules that protect β cells against ER stress-induced cell death. Mouse βTC6 cells were ...
Han Yangyang Y State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; Plastic Surgery Institute of Weifang Medical University, Weifang, Shandong 261041, PR China. Electronic address: - - 2014
Expansins are cell wall proteins inducing cell wall loosening and participate in all plant growth and development processes which are associated with cell wall modifications. Here, TaEXPB23, a wheat expansin gene, was investigated and the tolerance to oxidative stress was strongly enhanced in over-expression tobacco plants. Our results revealed that ...
Yurdakok Begum B Department of Pharmacology and Toxicology, Ankara University Faculty of Veterinary Medicine, Ankara 06110, - - 2014
Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study ...
Moustafa Khaled K a Institut National de la Santé et de la Recherche Médicale (INSERM) , Créteil , - - 2014
Abstract Thanks to their distinctive mode of action in a coordinated switch-like way, their multi-tiered signaling cascades and their involvement in cell responses to multiple internal and external stimuli, MAP kinases offer a remarkable possibility to be assembled into what we can call "MAPK transgenic circuits" to improve cell functions. ...
Wang Xuan X Department of Medical Cell Biology, Uppsala University , Uppsala , - - 2014
Abstract Background. Sodium palmitate causes apoptosis of β-cells, and the anti-apoptotic protein Bcl-2 has been shown to counteract this event. However, the exact mechanisms that underlie palmitate-induced pancreatic β-cell apoptosis and through which pathway Bcl-2 executes the protective effect are still unclear. Methods. A stable Bcl-2-overexpressing RINm5F cell clone (BMG) ...
Batnasan Enkhzaya E Key Laboratory of Molecular Epigenetics of the Ministry of Education, Changchun, 130024, - - 2014
Oxidative stress-induced DNA damage results in over-activation of poly(ADP-ribose) polymerase 1 (PARP1), leading to parthanatos, a newly discovered cell elimination pathway. Inhibition of PARP1-dependent cell death has shown to improve the outcome of diseases, including stroke, heart ischemia, and neurodegenerative diseases. In the present study we aimed to detect whether ...
Hammels Caroline C Dept. of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience, European Graduate School of Neuroscience (EURON), Maastricht University, Maastricht, The - - 2014
The enzyme DNA methyltransferase 3a (Dnmt3a) is crucially involved in DNA methylation and recent studies have demonstrated that Dnmt3a is functionally involved in mediating and moderating the impact of environmental exposures on gene expression and behavior. Findings in rodents have suggested that DNA methylation is involved in regulating neuronal proliferation ...
McKinnon D D DD Department of Chemical and Biological Engineering, BioFrontiers Institute, University of Colorado, Boulder, Colorado 80303, - - 2014
Studies focused on understanding the role of matrix biophysical signals on cells, especially those when cells are encapsulated in hydrogels that are locally remodelled, are often complicated by appropriate methods to measure differences between the bulk and local material properties. From this perspective, stress-relaxing materials that allow long-term culture of ...
Solé Carme C Cell Signaling unit, Departament de Ciències Experimentals i de la Salut, Cell Signaling Research Group, Universitat Pompeu Fabra (UPF), Dr Aiguader 88, E-08003, Barcelona, - - 2014
Eukaryotic cells have developed sophisticated systems to constantly monitor changes in the extracellular environment and to orchestrate a proper cellular response. To maximize survival, cells delay cell-cycle progression in response to environmental changes. In response to extracellular insults, stress-activated protein kinases (SAPKs) modulate cell-cycle progression and gene expression. In yeast, ...
Liu Fei F 1State Key Laboratory of Mechanical Transmission,College of Mechanical Engineering,Chongqing University,Chongqing - - 2014
Mechanical properties are vital for living cells, and various models have been developed to study the mechanical behavior of cells. However, there is debate regarding whether a cell behaves more similarly to a "cortical shell - liquid core" structure (membrane-like) or a homogeneous solid (cytoskeleton-like) when experiencing stress by mechanical ...
Savion Naphtali N Goldschleger Eye Research Institute, Sackler Faculty of Medicine, Tel Aviv University, Sheba Medical Center, Tel Hashomer 52621, Israel. Electronic address: - - 2014
Oxidative stress and/or low cellular glutathione are associated with development and progression of neurodegenerative diseases. We have shown that S-allylmercapto-N-acetylcysteine (ASSNAC) up-regulates the level of glutathione and phase II detoxifying enzymes in cultured vascular endothelial cells. The present study demonstrates that exposure of nerve cell lines to ASSNAC significantly increases ...
Banerjee Priyanjalee P Department of Biochemistry, Institute of Postgraduate Medical Education and Research, 244, AJC Bose Road, Kolkata, 700020, - - 2014
The increased accumulation of iron in the brain in Alzheimer's disease (AD) is well documented, and excess iron is strongly implicated in the pathogenesis of the disease. The adverse effects of accumulated iron in AD brain may include the oxidative stress, altered amyloid beta-metabolism and the augmented toxicity of metal-bound ...
Lin Chia-Yu CY Department of Health and Nutrition Biotechnology, Asia University , Taichung, Taiwan - - 2014
Abstract Oxidative stress has been implicated in the etiology of pancreatic β-cell dysfunction and diabetes. Studies have shown that chlorella could be important in health promotion or disease prevention through its antioxidant capacity. However, whether chlorella has a cytoprotective effect in pancreatic β-cells remains to be elucidated. We investigated the ...
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