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Avilés C C a MERAGEM Research Group, Veterinary Faculty , University of Cordoba , Cordoba , - - 2015
Stearoyl-CoA desaturase (SCD1 gene) is an enzyme responsible for the endogenous conversion of saturated fatty acid into monounsaturated fatty acids. The objective of this study was to assess the association of a single nucleotide polymorphism (SNP) in the SCD1 gene with the fatty acid composition of beef intramuscular fat of ...
Wu Yun Y Department of Biomedical Engineering, University at Buffalo, State University of New York, 332 Bonner Hall, Buffalo, NY, 14260, USA, - - 2015
Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acids-based therapeutics. We present a facile microwell array to mediate the delivery of nucleic acids carried by lipoplexes, which combines the advantages of lipoplexes as an efficient carrier system, the surface mediated ...
Frank Alon A Laboratory of Plant Science, Migal, Galilee Research Institute Ltd., P.O.B. 831, 11016, Kiryat Shmona, - - 2014
Methionine is a nutritionally essential sulfur-containing amino acid found at low levels in plant tissues. Yet, the factors that regulate its synthesis and accumulation in seeds are not fully known. Recent genetic studies demonstrate that Arabidopsis seeds are able to synthesize methionine de novo through the aspartate family pathway similarly ...
van de Bunt Bert - - 2014
Background Lactococcus lactis is a lactic acid bacterium that has been used for centuries in the production of a variety of cheeses, as these bacteria rapidly acidify milk and greatly contribute to the flavour of the fermentation end-products. After a short growth phase during cheese ripening L. lactis enters an ...
Indest Karl J KJ Environmental Laboratory, CEERD EP-P, U.S. Army Engineer Research and Development Center, 3909 Halls Ferry Road, Vicksburg, MS, 39180, USA, - - 2014
Previously, we demonstrated triacylglycerol (TAG) accumulation and the in vivo ability to catalyze esters from exogenous short chain alcohol sources in Gordonia sp. strain KTR9. In this study, we investigated the effects that putative lipase (KTR9_0186) and wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (WS/DGAT; KTR9_3844) gene knockouts had on TAG accumulation. Gene ...
Liu Ze Z Washington State - - 2014
Omega-3 fatty acids (n-3 FAs) are proposed to have many beneficial effects on human health. However, the mechanisms underlying their potential cancer preventative effects are unclear. G protein-coupled receptors (GPCRs) of the free fatty acid receptor (FFAR) family, FFA1/GPR40 and FFA4/GPR120, specifically bind n-3 FAs as agonist ligands. In this ...
Amiguet-Vercher Amélia A Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015, Lausanne, - - 2014
Natural genetic variation is crucial for adaptability of plants to different environments. Seed dormancy prevents precocious germination in unsuitable conditions and is an adaptation to a major macro-environmental parameter, the seasonal variation in temperature and day length. Here we report the isolation of IBO, a quantitative trait locus (QTL) that ...
Zhao Li L Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, - - 2014
To investigate the effects of berberine (BBR) and cinnamic acid (CA), the main active components in Jiaotai Pill (, JTP), on palmitic acid (PA)-induced intracellular triglyceride (TG) accumulation in NIT-1 pancreatic β cells. Cells were incubated in culture medium containing PA (0.25 mmol/L) for 24 h. Then treatments with BBR ...
Whiting Ellis - - 2014
A mild and efficient one-pot procedure is described to transform salicylaldoximes into salicylonitriles using Mitsunobu chemistry. The reactions proceed through the corresponding 1,2-benzisoxazoles that undergo the Kemp elimination in situ to generate the target salicylonitriles in excellent yields. The chemistry exhibits a broad scope, and the salicylonitriles can be readily ...
Cheng Jin-Tang JT Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. - - 2014
The enantioselective N-heterocyclic carbene-catalyzed [4 + 2] cyclocondensation of 2-aryl carboxylic acids and enones was developed, affording the corresponding chiral δ-lactones in good yields with good diastereo- and high enantioselectivities.
Liu Xiaoyan X Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China, - - 2014
In this study, citric acid was produced from waste cooking oil by Yarrowia lipolytica SWJ-1b. To get the maximal yield of citric acid, the compositions of the medium for citric acid production were optimized, and our results showed that extra nitrogen and magnesium rather than vitamin B1 and phosphate were ...
Lavrenčič A A 1Department of Animal Science, Biotechnical Faculty,University of Ljubljana,Groblje 3,SI-1230 - - 2014
The aim of this study was to assess the effects of hop cones (Humulus lupulus L.) from two varieties Aurora and Dana, differing in their α- and β-acid contents, on rumen microbial activity measured with in vitro gas production kinetics and short-chain fatty acids (SCFA) production. Hop cones were added ...
Ronneau Severin S UNamur , URBM 61 rue de Bruxelles, Namur , Belgium - - 2014
Abstract The brucellae are α-Proteobacteria causing brucellosis, an important zoonosis. Although multiplying in endoplasmic reticulum-derived vacuoles, they cause no cell death, suggesting subtle but efficient use of host resources. Brucellae are amino-acid prototrophs able to grow with ammonium or use glutamate as the sole carbon-nitrogen source in vitro. They contain ...
Lichtenberger Lenard M LM Univ of Texas Med School - - 2014
Indomethacin is a powerful analgesic NSAID, but is limited in use by its side effect to cause gastrointestinal bleeding. One factor important for exacerbating NSAID injury is the presence of bile acids which may interact with indomethacin to form toxic mixed micelles in the gut. The development of a GI-safer ...
Liu Zheng Z Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong - - 2014
Acylation of proteins with fatty acids is important for the regulation of membrane association, trafficking, subcellular localization, and activity of many cellular proteins. While significant progress has been made in our understanding of the two major forms of protein acylation with fatty acids, N-myristoylation and S-palmitoylation, studies of the acylation ...
Hwang Hee Jin HJ Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, South - - 2014
The dynamics of the membrane fatty acid composition of a succinic acid-producing bacterium, Anaerobiospirillum succiniciproducens, was studied using continuous and batch cultures. In both batch and chemostat continuous culture conditions, A. succiniciproducens grown on glucose or glycerol responded to acid stress by lowering the content of saturated fatty acids (SFAs) ...
Jeong Seongkeun S College of Pharmacy, Pusan National University, Busan, Republic of Korea, - - 2014
Inhibition of hypoxia inducible factor-prolyl hydroxylase-2 (HPH), leading to activation of hypoxia inducible factor (HIF)-1, is a potential therapeutic strategy for the treatment of colitis. Rosmarinic acid (RA), an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid, is a naturally occurring polyphenolic compound with two catechols, a pharmacophore for inhibition of ...
Sheoran Sunita S Department of Pharmacognosy and Phytochemistry, Microbial and Pharmaceutical Biotechnology Laboratory, Centre for Advanced Research in Pharmaceutical Science, Faculty of Pharmacy, Jamia Hamdard, New Delhi, - - 2014
Gymnema sylvestre is an important anti-diabetic medicinal plant, hence it is necessary to study the effective extraction of its active medicinal components. To develop an efficient ultrasound-assisted extraction method for anti-diabetic gymnemic acids from Gymnema sylvestre leaves and measure their effect on insulin-producing RINm-5 F β cells. Box-Behnken's design and response ...
Li Guihua - - 2014
Aggregation behaviors of the mixtures of lysine and fatty acids (FAs) with different chain lengths in aqueous solutions were investigated and the self-assembled structural transition was determined in detail. Aggregates including micelles, vesicles, sponge structures, and fibers were observed by varying the compositions and the chain length of fatty acids. ...
Choi Yun-Seok YS Colorado State University, United - - 2014
The ubiquitin E2 enzymes, Ube2g1 and Ube2r1, are able to synthesize K48-linked poly-ubiquitins without an E3 ligase, but how that is accomplished has been unclear. Although both E2s contain essential acidic loops, only Ube2r1 requires an additional C-terminal extension (184-196) for efficient K48-ubiquitylation activity. The presence of Y102 and Y104 ...
Liu Helu - - 2014
Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in important physiological processes. However, endogenous PUFA biosynthesis pathway is poorly understood in marine bivalve. Previously, a fatty acyl desaturase (Fad) with Δ5 activity was functionally characterized and an elongase termed as Elovl2/5 was reported to efficiently elongate 18:2n-6 and 18:3n-3 to 20:2n-6 ...
Kang Hyun Mi HM Renal Electrolyte and Hypertension Division, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, - - 2014
Renal fibrosis is the histological manifestation of a progressive, usually irreversible process causing chronic and end-stage kidney disease. We performed genome-wide transcriptome studies of a large cohort (n = 95) of normal and fibrotic human kidney tubule samples followed by systems and network analyses and identified inflammation and metabolism as ...
Li Wei W School of Biotechnology, Yeungnam University , Gyeongsan, Gyeongbuk 712-749, - - 2014
Six new (erinarols A-F, 1-6) and five known (7-11) ergostane-type sterol fatty acid esters were isolated from the methanol extract of the dried fruiting bodies of Hericium erinaceum. Their chemical structures were elucidated using chemical and physical methods as well as through comparison of NMR and mass spectral data with ...
Zhu Yingdong - - 2014
Growing evidence has shown that ascorbic acid (ASA) can contribute to protein glycation and the formation of advanced glycation end products (AGEs), especially in the lens. The mechanism by which ascorbic acid can cause protein glycation probably originates from its oxidized form, dehydroascorbic acid (DASA), which is a reactive dicarbonyl ...
Häusler Rainer E RE Department of Botany II, University of Cologne, Cologne Biocenter, Zülpicherstr. 47B, 50674 Cologne, Germany. Electronic address: - - 2014
Amino acids serve as constituents of proteins, precursors for anabolism, and, in some cases, as signaling molecules in mammalians and plants. This review is focused on new insights, or speculations, on signaling functions of serine, γ-aminobutyric acid (GABA) and phenylalanine-derived phenylpropanoids. Serine acts as signal in brain tissue and mammalian ...
Huang Yue Y CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, - - 2014
Two human demethylases, the fat mass and obesity-associated (FTO) enzyme and ALKBH5, oxidatively demethylate abundant N(6)-methyladenosine (m(6)A) residues in mRNA. Achieving a method for selective inhibition of FTO over ALKBH5 remains a challenge, however. Here, we have identified meclofenamic acid (MA) as a highly selective inhibitor of FTO. MA is ...
Taylor Alexander I AI MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, - - 2014
The emergence of catalysis in early genetic polymers such as RNA is considered a key transition in the origin of life, pre-dating the appearance of protein enzymes. DNA also demonstrates the capacity to fold into three-dimensional structures and form catalysts in vitro. However, to what degree these natural biopolymers comprise ...
Sonar Nitin R NR Department of Food Microbiology, CSIR-Central Food Technological Research Institute, Mysore, 570 020 - - 2014
Fermented bamboo shoots such as Soibum, Soidon, Eup, Hirring, Hecche and Ekung etc. are non-salted acidic products obtained by natural fermentation predominantly with lactic acid bacteria (LAB). In this study, we have characterized 11-representative LAB that includes, Lactobacillus sp. (n = 2), Lactobacillus plantarum (n = 3), and one each of Lactobacillus fermentum, Lactococcus ...
Takeuchi Michiki M Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, - - 2014
Linoleic acid Δ9 hydratase, which is involved in linoleic acid saturation metabolism of Lactobacillus plantarum AKU 1009a, was cloned, expressed as a his-tagged recombinant enzyme, purified with an affinity column, and characterized. The enzyme required FAD as a cofactor and its activity was enhanced by NADH. The maximal activities for ...
Ali Md Sarafat MS School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712-749, - - 2014
We report the significant increase of the content of free amino acids in Nicotiana benthamiana by the co-suppression of the ClpC1 and ClpC2 genes, which are translated to be the chaperonic part in the Clp protease at plastids. Clp protease with ClpC1 and ClpC2 proteins as the chaperonic part degrades ...
Bala Manju M a Academy of Scientific & Innovative Research , New Delhi , - - 2014
Eleven constituents were characterised by gas chromatography-mass spectrometry analysis, and five molecules were isolated using column chromatography. The in vitro study of the extract and isolated molecules against KB and SiHa cell lines revealed oleanolic acid (1) and oleic acid (2) as potent cytotoxic molecules with potential anticancer activity. The ...
Park Sang Ho SH Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093-0307, - - 2014
The use of paramagnetic constraints in protein NMR is an active area of research because of the benefits of long-range distance measurements (>10 Å). One of the main issues in successful execution is the incorporation of a paramagnetic metal ion into diamagnetic proteins. The most common metal ion tags are relatively ...
Liang Shengzong S Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, USA. bo.xu@louisville.edu - - 2014
The combined acid catalyzed hydration of alkynes is an efficient one-step synthesis of carbonyl compounds. This atom-economical method is effective with a wide range of substrates, and the products are obtained in very good yields with low catalyst loading (0.2%). Furthermore, solid acids like Nafion were also efficient and could ...
Sun Chong C Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR - - 2014
The development of advanced nanomaterials for the highly efficient electrical detection of biological species has attracted great attention. Here, novel polypyrrole-Pluronic F127 nanoparticles (PPy-F127 NPs) with conducting and biocompatibility properties were synthesized and used to construct a l-lactic acid biosensor that could be applied in biochemical assays. The PPy-F127 NPs ...
Lee Jong Wha JW Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea. - - 2014
The effect of acids on the structure of lysozyme (Lyz) during electrospray ionization (ESI) was studied by comparing the solution and gas-phase structures of Lyz. Investigation using circular dichroism spectroscopy and small-angle X-ray scattering demonstrated that the folded conformation of Lyz was maintained in pH 2.2 solutions containing different acids. ...
Chen Fang - - 2014
An amphiphilic fatty acid oat β-glucan ester (FAOGE) was firstly synthesized and its structure-curcumin loading capacity (CLC) relationship was investigated. The DS of product increased with the addition of acyl imidazole, decreased with Mw of β-glucan and did not relate to the acyl chain length. Characterizations by FT-IR and 1H-NMR ...
Wang Guang-Yuan GY Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, - - 2014
Yarrowia lipolytica ACA-DC 50109 is an oleaginous yeast. In order to know the function of pyruvate carboxylase (PYC) in lipid biosynthesis, the PYC gene cloned from Pichia guilliermondii Pcla22 was overexpressed in the oleaginous yeast. The lipid contents in the wild-type strain ACA-DC 50109 and the transformants P4, P7, and ...
Ma Dong Hee DH Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, 790-784, Republic of Korea. - - 2014
The selective detection of cysteine and homocysteine over glutathione and other amino acids was demonstrated with an 8-MeS-BODIPY probe by mimicking the native chemical ligation approach, which allowed the selective and ratiometric fluorescence sensing of cysteine over other biothiols at physiologically relevant concentrations and in different organs of a zebrafish.
Kumar Ramesh R Environmental and Membrane Technology Laboratory, Department of Chemical Engineering, National Institute of Technology Durgapur, 713209, - - 2014
Experimental investigations were carried out on continuous and direct production of poly-(γ-glutamic acid) in a hybrid reactor system that integrated conventional fermentative production step with membrane-based downstream separation and purification. Novelty of the integrated system lies in high degree of purity, conversion, yield and productivity of poly-(γ-glutamic acid) through elimination ...
de Jong Bouke Wim BW Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, 41296, Sweden, - - 2014
Fatty acid ethyl esters are fatty acid derived molecules similar to first generation biodiesel (fatty acid methyl esters; FAMEs) which can be produced in a microbial cell factory. Saccharomyces cerevisiae is a suitable candidate for microbial large scale and long term cultivations, which is the typical industrial production setting for ...
Jia Hai-Yang HY a Department of Pharmaceutical Engineering , School of Chemistry and Chemical Engineering, Southeast University , Nanjing 211189 , PR - - 2014
A phytochemical investigation of the ethanolic extracts of the dried roots of Euphorbia nematocypha resulted in the isolation of a new phenylpropanoid, 16-O-caffeoyl-16-hydroxylhexadecanoic acid (1), together with 23 known compounds (2-24). Their structures were elucidated on the basis of spectroscopic data. Compound 1 was first to be isolated as a ...
Rohm Barbara B Christian Doppler Laboratory for Bioactive Aroma Compounds, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. - - 2014
Red pepper and its major pungent component, capsaicin, have been associated with hypolipidemic effects in rats, although mechanistic studies on the effects of capsaicin and/or structurally related compounds on lipid metabolism are scarce. In this work, the effects of capsaicin and its structural analog nonivamide, the aliphatic alkamide trans-pellitorine and ...
Suen Yung Lee - - 2014
Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on the cell growth, fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp.. The hemoglobin of Vitreoscilla stercoraria (VHb) gene ...
Wang Hai-Yang HY a Key Laboratory of Rare and Uncommon Diseases, Institute of Materia Medica, Shandong Academy of Medical Sciences , Ji'nan , Shandong 250062 , PR - - 2014
Two new triterpenoids and three 27-nor-triterpenoids were isolated from the stems (with bark) of Nauclea officinalis. Their structures were identified to be 2β,3β,19α,23-tetrahydroxy-urs-12-en-28-oic acid (1), 2β,3β,19α,23-tetrahydroxy-urs-12-en-28-O-[β-d-glucopyranosyl (1-2)-β-d-glucopyranosyl] ester (2), pyrocincholic acid 3β-O-α-l-rhamnopyranoside (3), pyrocincholic acid 3β-O-α-l-rhamnopyranosy1-28-O-β-d-glucopyranosyl ester (4), pyrocincholic acid 3β-O-α-l-rhamnopyranosy1-28-O-β-d-glucopyranosyl-(1-6)-β-d-glucopyranosyl ester (5) by spectroscopic methods including 1D, 2D NMR ...
Nikaido Yoshiaki Y Faculty of Science and Technology, Division of Molecular Science, Gunma University, Gunma 376-8515, - - 2014
GPR84 is a G protein-coupled receptor for medium-chain fatty acids. Capric acid and 3,3'-diindolylmethane are specific agonists for GPR84. We built a homology model of a GPR84-capric acid complex to investigate the ligand binding mode using the crystal structure of human active-state β2-adrenergic receptor. We performed site-directed mutagenesis to subject ...
Snyder Nathaniel W NW University of Pennsylvania, United - - 2014
Metabolism of propionate involves the activated acyl-thioester propionyl-coenzyme A (CoA) intermediate. We employed liquid chromatography-tandem mass spectrometry (LC-MS/MS), LC-selected reaction monitoring (SRM)/MS and LC- high-resolution MS (HRMS) to investigate metabolism of propionate to acyl-CoA intermediates. We discovered that propionyl-CoA can serve as a precursor to the direct formation of a ...
Ouml Oumlrni Katariina K Wihuri Research Institute, Finland - - 2014
Atherosclerotic lesions are often hypoxic and exhibit elevated lactate concentrations and local acidification of the extracellular fluids. The acidification may be a consequence of the abundant accumulation of lipid-scavenging macrophages in the lesions. Activated macrophages have a very high energy demand and they preferentially use glycolysis for ATP synthesis even ...
Leber Christopher C Department of Chemical Engineering and Materials Science, The Henry Samueli School of Engineering, University of California, Irvine, CA, - - 2014
The production of fuels and chemicals from biorenewable resources is important to alleviate the growing environmental concerns, costs, and foreign dependency associated with the use of petroleum feedstock. Fatty acids are attractive biomolecules due to the flexibility of their iterative biosynthetic pathway, high energy content, and suitability for conversion into ...
Ilko David D Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, 97074 Wuerzburg, - - 2014
The fatty acid (FA) composition of polysorbate 80 (PS80), a sorbitan oleic acid ester copolymerized with about 20mole of ethylene oxide, is typically characterized by gas chromatography. Here, an alternative method was developed. After saponification with potassium hydroxide the FA fraction was collected with liquid-liquid extraction using methyl-tert-butyl ether. HPLC ...
Kim Eui-Jin - - 2014
Cellular fatty acid composition is important for metabolic plasticity in Rhodobacter sphaeroides. We explored the effects of changing the cellular ratio of unsaturated fatty acids (UFA) to saturated fatty acids (SFA) in R. sphaeroides by overexpressing several key fatty acid biosynthetic enzymes through the use of expression plasmid pRK415. Bacteria ...
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